Anupam Kumari - Profile on Academia.edu (original) (raw)

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Chaudhary Charan Singh Haryana Agricultural University - Hisar (INDIA)

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Papers by Anupam Kumari

Research paper thumbnail of TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Indian Journal of Scientific Research, 2020

Tissue culture study was performed to standardize the technique and select suitable plant growth ... more Tissue culture study was performed to standardize the technique and select suitable plant growth regulators and their concentrations for efficient callus induction from the nodal explants of Asparagus racemosus willd, an important medicinal herb. Different concentrations of NAA, 2,4-D, BAP and KN were supplemented in MS basal medium in which the nodal explants were inoculated. It was observed that MS + 0.2 mg/l NAA was the best culture condition in which maximum percentage of response 90.36 was obtained. This was followed by the culture conditions that were MS+ 2.0 mg/l where the percentage response was 88.68%. Similarly, next higher percentage 88.62 was observed in MS+1.0 mg/l BAP + 1.0 mg/l NAA. It was further observed that growth rate was excellent where the percentage response was higher. Second it was noted that the calli had both compact and friable texture and their colour varied from green yellow to brown even where the growth was excellent. It was further noted that NAA or BAP had no response for callusing when used alone. However, KN at higher concentration 2.0 mg/l gave the highest percentage of response for callusing. NAA + BAP and NAA + KN at the similar concentrations gave different result. In the MS medium having 1.0 mg/l BAP + 1.0 mg/l NAA the percentage of response was 88.62 while MS + 1.0 mg/l KN + 1.0 mg/l NAA the percentage of response was 62.34 only. Growth rate of the calli also differed in different culture conditions.

++++ Excellent, + ++ Best, + + Better, + Growth DISCUSSION rather there was no response for callusing in MS + 2.0 mg/l NAA. This was also noted in case of MS + 0.5 mg/l 2,4-D where there was maximum response and at MS + 0.2 mg/l BAP alone the response was better but at higher concentration there was no response at all. Just reverse to the above findings MS + KN at higher concentration maximum percentage of response for callus induction 88.78 was found. It was further noted that MS + 1.0 mg/l NAA + 1.0 mg/l BAP gave better response, but either NAA or BAP at 1.0 mg/l had lower response. This may be due to the fact that for callusing, interaction of mg/l NAA. This was also noted in case of MS + 0.5 mg/l In the present study, experiments were done to

Research paper thumbnail of TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Indian Journal of Scientific Research, 2020

Tissue culture study was performed to standardize the technique and select suitable plant growth ... more Tissue culture study was performed to standardize the technique and select suitable plant growth regulators and their concentrations for efficient callus induction from the nodal explants of Asparagus racemosus willd, an important medicinal herb. Different concentrations of NAA, 2,4-D, BAP and KN were supplemented in MS basal medium in which the nodal explants were inoculated. It was observed that MS + 0.2 mg/l NAA was the best culture condition in which maximum percentage of response 90.36 was obtained. This was followed by the culture conditions that were MS+ 2.0 mg/l where the percentage response was 88.68%. Similarly, next higher percentage 88.62 was observed in MS+1.0 mg/l BAP + 1.0 mg/l NAA. It was further observed that growth rate was excellent where the percentage response was higher. Second it was noted that the calli had both compact and friable texture and their colour varied from green yellow to brown even where the growth was excellent. It was further noted that NAA or BAP had no response for callusing when used alone. However, KN at higher concentration 2.0 mg/l gave the highest percentage of response for callusing. NAA + BAP and NAA + KN at the similar concentrations gave different result. In the MS medium having 1.0 mg/l BAP + 1.0 mg/l NAA the percentage of response was 88.62 while MS + 1.0 mg/l KN + 1.0 mg/l NAA the percentage of response was 62.34 only. Growth rate of the calli also differed in different culture conditions.

++++ Excellent, + ++ Best, + + Better, + Growth DISCUSSION rather there was no response for callusing in MS + 2.0 mg/l NAA. This was also noted in case of MS + 0.5 mg/l 2,4-D where there was maximum response and at MS + 0.2 mg/l BAP alone the response was better but at higher concentration there was no response at all. Just reverse to the above findings MS + KN at higher concentration maximum percentage of response for callus induction 88.78 was found. It was further noted that MS + 1.0 mg/l NAA + 1.0 mg/l BAP gave better response, but either NAA or BAP at 1.0 mg/l had lower response. This may be due to the fact that for callusing, interaction of mg/l NAA. This was also noted in case of MS + 0.5 mg/l In the present study, experiments were done to

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