Arun Pal - Academia.edu (original) (raw)
Papers by Arun Pal
IJIEM - Indonesian Journal of Industrial Engineering and Management
The problem of operational efficiency assessment of medical equipment is becoming crucial, due to... more The problem of operational efficiency assessment of medical equipment is becoming crucial, due to its increasing requirement in hospitals. It has been observed that a significant amount of medical equipment is out of service for several reasons such as lack of training, maintenance and health technology management. The unexpected failures, downtime associated with breakdown and make ready, loss of production and poor maintenance costs of medical equipment are the major drawback in any hospital. Quality of diagnostic and treatment care provided to patients largely depends on the reliability, availability and maintainability of sophisticated medical equipment. Aim of the present study is to determine quantitatively overall effectiveness and utilization of some medical equipment. Overall Equipment Effectiveness (OEE) and utilization coefficient is the metric measurement of Total Productive Maintenance (TPM) which specifies effective functioning of devices. The results of the effective...
Journal of Statistical Computation and Simulation
Cells, 2022
Little is known about the early pathogenic events by which mutant superoxide dismutase 1 (SOD1) c... more Little is known about the early pathogenic events by which mutant superoxide dismutase 1 (SOD1) causes amyotrophic lateral sclerosis (ALS). This lack of mechanistic understanding is a major barrier to the development and evaluation of efficient therapies. Although protein aggregation is known to be involved, it is not understood how mutant SOD1 causes degeneration of motoneurons (MNs). Previous research has relied heavily on the overexpression of mutant SOD1, but the clinical relevance of SOD1 overexpression models remains questionable. We used a human induced pluripotent stem cell (iPSC) model of spinal MNs and three different endogenous ALS-associated SOD1 mutations (D90Ahom, R115Ghet or A4Vhet) to investigate early cellular disturbances in MNs. Although enhanced misfolding and aggregation of SOD1 was induced by proteasome inhibition, it was not affected by activation of the stress granule pathway. Interestingly, we identified loss of mitochondrial, but not lysosomal, integrity as...
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Illustration of the object recognition and tracking analysis. Representative examples of Ctrl, FU... more Illustration of the object recognition and tracking analysis. Representative examples of Ctrl, FUS and TDP43 axon images at the distal readout (Mock) obtained with Mitotracker (left) and Lysotracker (right), scale bar = 10µm. Note the high coverage of the FIJI Track Mate object recognition (pink circles) and tracking tool (yellow lines) as nearly every organelle is detected. White arrowheads point to examples of rare false positives outside microchannels that were removed through subsequent post filtering (track duration ≥ 3s, track displacement ≥ 1.2µm), see Methods, section 'Automated object recognition and dynamic tracking with the FIJI Track Mate plugin' and 'Data mining in CSV result files and assembly of final EXCEL result tables with KNIME'.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
MS Excel table containing Z-scores of all parameters to build the multiparametric phenotypic sign... more MS Excel table containing Z-scores of all parameters to build the multiparametric phenotypic signatures as described in the main manuscript under Methods, section 'Assembly of phenotypic HC signatures'.
Illustration of absent dendrites at the distal and proximal readout position inside MFC microchan... more Illustration of absent dendrites at the distal and proximal readout position inside MFC microchannels. Representative example of Ctrl Mock iPSC-derived human spinal motor neurons matured over 3 weeks in MFCs showing IF immunostainings for the dendritic marker MAP2 (in pink) and the motor neuron marker SMI32 (turquoise). Note the absence of any MAP2-positive neurites inside channels at either the distal and proximal site where the movie acquisition was performed. Only a few MAP2-positive ramifications were reaching out from the open proximal field to the vicinity of proximal channel entries but remained outside. Scale bar = 10µm.
Illustration of absent dendrites at the distal and proximal readout position inside MFC microchan... more Illustration of absent dendrites at the distal and proximal readout position inside MFC microchannels. Representative example of Ctrl Mock iPSC-derived human spinal motor neurons matured over 3 weeks in MFCs showing IF immunostainings for the dendritic marker MAP2 (in pink) and the motor neuron marker SMI32 (turquoise). Note the absence of any MAP2-positive neurites inside channels at either the distal and proximal site where the movie acquisition was performed. Only a few MAP2-positive ramifications were reaching out from the open proximal field to the vicinity of proximal channel entries but remained outside. Scale bar = 10µm.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
FIJI macro code (ijm file) of static organelle morphology analysis as described in Methods, secti... more FIJI macro code (ijm file) of static organelle morphology analysis as described in Methods, section 'Automated object recognition and static morphology analysis with the FIJI Morphology macro'.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
IJIEM - Indonesian Journal of Industrial Engineering and Management
The problem of operational efficiency assessment of medical equipment is becoming crucial, due to... more The problem of operational efficiency assessment of medical equipment is becoming crucial, due to its increasing requirement in hospitals. It has been observed that a significant amount of medical equipment is out of service for several reasons such as lack of training, maintenance and health technology management. The unexpected failures, downtime associated with breakdown and make ready, loss of production and poor maintenance costs of medical equipment are the major drawback in any hospital. Quality of diagnostic and treatment care provided to patients largely depends on the reliability, availability and maintainability of sophisticated medical equipment. Aim of the present study is to determine quantitatively overall effectiveness and utilization of some medical equipment. Overall Equipment Effectiveness (OEE) and utilization coefficient is the metric measurement of Total Productive Maintenance (TPM) which specifies effective functioning of devices. The results of the effective...
Journal of Statistical Computation and Simulation
Cells, 2022
Little is known about the early pathogenic events by which mutant superoxide dismutase 1 (SOD1) c... more Little is known about the early pathogenic events by which mutant superoxide dismutase 1 (SOD1) causes amyotrophic lateral sclerosis (ALS). This lack of mechanistic understanding is a major barrier to the development and evaluation of efficient therapies. Although protein aggregation is known to be involved, it is not understood how mutant SOD1 causes degeneration of motoneurons (MNs). Previous research has relied heavily on the overexpression of mutant SOD1, but the clinical relevance of SOD1 overexpression models remains questionable. We used a human induced pluripotent stem cell (iPSC) model of spinal MNs and three different endogenous ALS-associated SOD1 mutations (D90Ahom, R115Ghet or A4Vhet) to investigate early cellular disturbances in MNs. Although enhanced misfolding and aggregation of SOD1 was induced by proteasome inhibition, it was not affected by activation of the stress granule pathway. Interestingly, we identified loss of mitochondrial, but not lysosomal, integrity as...
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Illustration of the object recognition and tracking analysis. Representative examples of Ctrl, FU... more Illustration of the object recognition and tracking analysis. Representative examples of Ctrl, FUS and TDP43 axon images at the distal readout (Mock) obtained with Mitotracker (left) and Lysotracker (right), scale bar = 10µm. Note the high coverage of the FIJI Track Mate object recognition (pink circles) and tracking tool (yellow lines) as nearly every organelle is detected. White arrowheads point to examples of rare false positives outside microchannels that were removed through subsequent post filtering (track duration ≥ 3s, track displacement ≥ 1.2µm), see Methods, section 'Automated object recognition and dynamic tracking with the FIJI Track Mate plugin' and 'Data mining in CSV result files and assembly of final EXCEL result tables with KNIME'.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
MS Excel table containing Z-scores of all parameters to build the multiparametric phenotypic sign... more MS Excel table containing Z-scores of all parameters to build the multiparametric phenotypic signatures as described in the main manuscript under Methods, section 'Assembly of phenotypic HC signatures'.
Illustration of absent dendrites at the distal and proximal readout position inside MFC microchan... more Illustration of absent dendrites at the distal and proximal readout position inside MFC microchannels. Representative example of Ctrl Mock iPSC-derived human spinal motor neurons matured over 3 weeks in MFCs showing IF immunostainings for the dendritic marker MAP2 (in pink) and the motor neuron marker SMI32 (turquoise). Note the absence of any MAP2-positive neurites inside channels at either the distal and proximal site where the movie acquisition was performed. Only a few MAP2-positive ramifications were reaching out from the open proximal field to the vicinity of proximal channel entries but remained outside. Scale bar = 10µm.
Illustration of absent dendrites at the distal and proximal readout position inside MFC microchan... more Illustration of absent dendrites at the distal and proximal readout position inside MFC microchannels. Representative example of Ctrl Mock iPSC-derived human spinal motor neurons matured over 3 weeks in MFCs showing IF immunostainings for the dendritic marker MAP2 (in pink) and the motor neuron marker SMI32 (turquoise). Note the absence of any MAP2-positive neurites inside channels at either the distal and proximal site where the movie acquisition was performed. Only a few MAP2-positive ramifications were reaching out from the open proximal field to the vicinity of proximal channel entries but remained outside. Scale bar = 10µm.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
FIJI macro code (ijm file) of static organelle morphology analysis as described in Methods, secti... more FIJI macro code (ijm file) of static organelle morphology analysis as described in Methods, section 'Automated object recognition and static morphology analysis with the FIJI Morphology macro'.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.