Asep Supriadin - Academia.edu (original) (raw)

Papers by Asep Supriadin

Teknotan: Jurnal Industri Teknologi, Apr 15, 2022

Biji nangka dan biji durian merupakan limbah organik yang belum dimanfaatkan secara optimal. Biji... more Biji nangka dan biji durian merupakan limbah organik yang belum dimanfaatkan secara optimal. Biji durian mempunyai kandungan serat sebesar 22,48 % sedangkan biji nangka 7,46 %, yang dapat dimanfaatkan sebagai pangan fungsional. Senyawa oligosakarida yang terdapat dalam kedua biji tersebut dapat dijadikan sebagai sumber prebiotik pada bakteri asam laktat (BAL). Tujuan dari penelitian ini adalah untuk menganalisis efektivitas tepung biji nangka dan durian sebagai media pertumbuhan bakteri Lactobacillus casei. Pengujian hasil fermentasi tepung dilakukan pada jam ke 0, 24, 48, dan 72 dengan dilakukan analisis jumlah bakteri, pH, total asam, dan gula reduksi. Fermentasi 48 jam merupakan waktu puncak untuk pertumbuhan Lactobacillus casei. Jumlah bakteri pada tepung biji nangka sebanyak 3,24×10 10 sel/ml, sedangkan pada tepung biji durian 3,04 × 10 10 sel/ml. Nilai pH dari tepung biji nangka yaitu pH 3,8, sedangkan tepung biji durian 4,3. Total asam pada substrat tepung biji nangka yaitu 0,357 % dan 0,354 % pada tepung biji durian. Kadar gula reduksi pada tepung biji nangka 2,4658 mg/ml, sedangkan tepung biji durian 2,4925 mg/ml. Dengan membandingkan jumlah bakteri, nilai pH dan total asam diketahui bahwa tepung biji nangka lebih efektif sebagai prebiotik bagi bakteri L. casei daripada tepung biji durian.

IOP conference series, Mar 1, 2021

Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the a... more Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the application of production inputs should be directed to improve of those crop quality. The aim of the research was to know the effect of potassium concentration and type of planting media on production and quality of tomato (Lycopersicon esculentum) on hydroponic drip system. The research was conducted from June to August 2019 at Cileunyi Kulon village, Cilenyi district, Bandung regency, using split-plot design with 2 factors and 3 replications. Main plot was potassium concentration (350 ppm, 400 ppm, and 450 ppm) and sub-plot type of planting media (100 % husk charcoal, 100 % cocopeat, 50 % husk charcoal + 50 % cocopeat). The result showed that there was no. interaction effect of potassium concentration and type of planting media on fruit fresh weight and fruit hardness. The potassium application up to 450 ppm as well as the rice husk charcoal and cocopeat media produced the same effect on the fresh weight and the hardness of the fruit of tomatoes and its value were slightly below its potential in plant description. There are also the vitamin C levels obtained is in the low range of the vitamin C level.

Nucleation and Atmospheric Aerosols, 2023

The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps an... more The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps and the purity of enzymes was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results showed that, the enzyme was purified 4.7-fold with a specific activity of 134.6 U/mg protein and a yield of 44%. When it was subjected to SDS-PAGE, a single band near 58 kDa appeared. The optimum temperature and pH were 50°C and 7.0, respectively. The addition of Ca 2+ , Mn 2+ and Co 2+ could improve the enzyme activity, while Cu 2+ , Hg 2+ , Fe 3+ and Al 3+ decreased the activity. The enzyme was inhibited by ethylenediaminetetraacetic acid (EDTA), ethylenebis(oxonitrilo)]tetra-acetate (EGTA), SDS and dimethyl sulfoxide (DMSO), but was not affected by phenylmethane-sulfonyl fluoride (PMSF) and 1,4-dithiothreitol (DTT). K m and V max values of the purified enzyme for soluble starch were 1.73 ± 0.3 mg/ml and 1.24 ± 0.02 mg/ml/min, respectively. The degradation ability of wild type α-amylase on starch granules was examined by thin layer chromatography. The final purified enzyme had an isoelectric point of 7.5-7.8 and α-helix of 28%, β-sheet of 32% and random coil of 40%.

IOP conference series, Mar 1, 2021

Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants an... more Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants and vegetable storability. This study aims to determine the selenium content of baglog waste in increasing antioxidants and broccoli storability. The study was conducted from May to July 2019 in Cipadung Village, Cibiru District, Bandung using the Completely Randomized Design (CRD) consisted of 5 treatments and 5 replications. The treatment was media A = 100% baglog waste, B = baglog waste mixture 25%: 75% rice husk charcoal, C = Baglog waste mixture 50%: 50% rice husk charcoal, D = Baglog waste mixture 75%: 25% rice husk charcoal, E = 100% rice husk charcoal (control). The results showed that the combination of 75% baglog waste media with 25% rice husk charcoal was able to increase antioxidants by IC50 28.34 ppm and storability for four days at room temperature. 75% baglog waste with 25% husk charcoal can be used as a medium for supplying selenium to get good antioxidants and storability of broccoli.

Nucleation and Atmospheric Aerosols, 2023

Nucleation and Atmospheric Aerosols, 2023

Journal of physics, Dec 1, 2019

The impressed current cathodic protection is one of the effort to prevent a metal from corrosion ... more The impressed current cathodic protection is one of the effort to prevent a metal from corrosion by supplying electricity to metal objects. However, the protection system that supplies electricity must always be monitored so that the electricity intake to the metal object is sufficient to protect it from the corrosion. The way how to monitor the electricity intake to the metal is to read the current in taking on the metal. To read the electric current more easily is to use an application software. This research talks about the application design to display the electric current in taking on the metal through the computer screen. In order to read the electric current directly, the Advantect Data Acquisition Modul (ADAM) devices are used. The results show that the application is able to display the value of protection current for iron plate steel with size 30x10 cm are between 0.15 A to 0.18 A while for iron plate with a size of 30x13 cm are between 0.20 to 0.24 A. These values also avoid the over protection.

Materials Science Forum, Apr 1, 2021

Duration of use and injectability are external factors for Polydimethylsiloxane (PDMS) that needs... more Duration of use and injectability are external factors for Polydimethylsiloxane (PDMS) that needs to be considered when PDMS utilized as a vitreous substituted liquid in vitreoretinal surgery. In general, PDMS which has been used as a substitute for vitreous humour is PDMS with a low viscosity in the value about 1000 cSt and a high viscosity at a value of about 5000 cSt. Various deficiencies have been encountered from low and high viscosity of PDMS, causing research to be continued to obtain PDMS which has suitable properties as a substitute for vitreous humour. One of them is research to obtain medium viscosity of PDMS with a viscosity value of about 2000 cSt. Here, we reported synthesis and characterization of PDMS with medium viscosity in ranges from 1800 to 2600 mPas. PDMS was synthesized through Ring-Opening Polymerization (ROP) pathway using the monomer of Octamethylcyclotetrasiloxane (D4) and the chain terminator of Hexamethyldisiloxane (MM). Various concentrations of potassium hydroxide (KOH) of 3, 4, 6 and 8 %(w/v) were applied as initiator to form gel of PDMS. All synthesized PDMS samples were identified to have viscosity values of 1800-2600 mPas, refractive index values of 1.4042-1.4044 and surface tension values of 22-23 mN/m. Meanwhile, the results of Fourier-Transform Infrared (FTIR) measurement showed that the absorption peaks were similar to that of our previous report.

Journal of physics, Apr 1, 2021

The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is ... more The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is contained several secondary metabolites such as olive oil, phenolic compounds, flavonoids, triterpen, biphenol. The aims of this research are to isolate and identify a flavonoid compound that extracted in the ethyl acetate of olive leaves. Isolation was carried out by meseration, liquid vacuum chromatography, column chromatography and thin layer chromatography. Isolation results obtained 10 mg isolates were identified using proton nuclear magnetic spectroscopy (1H-NMR) and carbon (13C-NMR). According to spectra analysis, describe that isolate is chrysoeriol.

PROCEEDINGS OF THE SYMPOSIUM ON ADVANCE OF SUSTAINABLE ENGINEERING 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research

PROCEEDINGS OF THE SYMPOSIUM ON ADVANCE OF SUSTAINABLE ENGINEERING 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research

Jurnal Informatika, Jun 10, 2020

Gunung Djati Conference Series, Jul 1, 2021

IOP Conference Series: Materials Science and Engineering, 2021

Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the a... more Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the application of production inputs should be directed to improve of those crop quality. The aim of the research was to know the effect of potassium concentration and type of planting media on production and quality of tomato (Lycopersicon esculentum) on hydroponic drip system. The research was conducted from June to August 2019 at Cileunyi Kulon village, Cilenyi district, Bandung regency, using split-plot design with 2 factors and 3 replications. Main plot was potassium concentration (350 ppm, 400 ppm, and 450 ppm) and sub-plot type of planting media (100 % husk charcoal, 100 % cocopeat, 50 % husk charcoal + 50 % cocopeat). The result showed that there was no. interaction effect of potassium concentration and type of planting media on fruit fresh weight and fruit hardness. The potassium application up to 450 ppm as well as the rice husk charcoal and cocopeat media produced the same effect o...

Journal of Physics: Conference Series, 2021

The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is ... more The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is contained several secondary metabolites such as olive oil, phenolic compounds, flavonoids, triterpen, biphenol. The aims of this research are to isolate and identify a flavonoid compound that extracted in the ethyl acetate of olive leaves. Isolation was carried out by meseration, liquid vacuum chromatography, column chromatography and thin layer chromatography. Isolation results obtained 10 mg isolates were identified using proton nuclear magnetic spectroscopy (1H-NMR) and carbon (13C-NMR). According to spectra analysis, describe that isolate is chrysoeriol.

Journal of Physics: Conference Series, 2019

Internal corrosion in the piping system is a serious problem. The use of corrosion inhibitors for... more Internal corrosion in the piping system is a serious problem. The use of corrosion inhibitors for corrosion control in piping systems is a practical and cost-effective method. Many polymers have been reported to be used as an inhibitor corrosion. However, there was little attention to the research of Polyethylene Oxide (PEO) as a corrosion inhibitor in NaCl medium. The purpose of this study was to determine the corrosion inhibition activity of polymeric compounds of polyethylene oxide against ferrous metals in 1% NaCl electrolyte solution. The study of Polyethylene Oxide (PEO) activity as a corrosion inhibitor in 1% NaCl electrolyte solution was carried out by the Wheel Test method with variations in concentration and temperature. The surface analysis of iron by SEM (Scanning Electron Microscopy). The results showed that polyethylene oxide (PEO) had the optimum inhibitory activity at a concentration of 16 ppm, with inhibition efficiency of 80.15% at 25°C. Polyethylene Oxide has adso...

IOP Conference Series: Earth and Environmental Science, 2019

Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been ... more Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been known and used as medicines. Exploration on torch ginger plant needs to be done to conservate and determine genetic diversity. Study of genetic diversity torch ginger in the district of Pangandaran is important because as long as there has been no study in depth the potential of genetic diversity and phylogenetic relationship germplasm of torch ginger Genetic diversity based on this research had a proportion of 47% of the total of 100% in the PC1-PC3 was based on the analysis Principal Component Analysis (PCA) with the character influencing that form clumps, line leaf midrib, plant height, stem diameter, leaf length, color scales rhizome, stem color, rhizome and the leaf base forms. Based on the results of the genetic diversity cluster analysis found that narrow torch ginger euclidean indicated by coefficient of 0.79 and was divided into six clusters. Accession which had the closest kins...

IOP Conference Series: Materials Science and Engineering, 2018

Graphite is a natural carbon source that can be used for graphene synthesis because of its abunda... more Graphite is a natural carbon source that can be used for graphene synthesis because of its abundant availability and relatively low cost. In this research, Graphene Oxide (GO) and reduced Graphene Oxide (rGO) have been prepared through modified Hummers method that uses microwave irradiation and hydrothermal process as reduction methods. This research can be divided into two main steps, i.e. preparation of GO and preparation of rGO. The preparation of GO was done using modified Hummers method that refers to graphite powder oxidation for five days. Then, preparation of rGO was done using two methods, i.e. microwave irradiation for 8 minutes at 1,000 watts and hydrothermal process for 10 hours at 200°C. The characterization of the samples was observed by Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Ultraviolet-Visible Spectroscopy (UV-Vis). XRD pattern of GO sample shows a peak at 2θ = 10.63°, rGO sample from hydrothermal process at 2θ = 25.94°, and rGO sample from microwave irradiation method at 2θ = 25.94° with low intensity and at 2θ = 10.63° that indicates the presence of GO. IR spectroscopy data shows an absorption peak of aromatic C=C at 1,573 cm-1 that indicates rGO formation. SEM images of GO illustrates a structure of stacked flakes. While the morphology of rGO has a structure of stacked flakes that is more transparent than GO. According to UV-Vis spectroscopy, GO and rGO of samples show absorption peak at λmax = 225 nm and λmax = 274 nm, respectively, which indicates π→π* transition of aromatic C=C bond. Based on the results of this study, the preparation of rGO using modified Hummers that combined with hydrothermal reduction method is more effective than microwave irradiation reduction method.

African Journal of Biotechnology, 2011

The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps an... more The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps and the purity of enzymes was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results showed that, the enzyme was purified 4.7-fold with a specific activity of 134.6 U/mg protein and a yield of 44%. When it was subjected to SDS-PAGE, a single band near 58 kDa appeared. The optimum temperature and pH were 50°C and 7.0, respectively. The addition of Ca 2+ , Mn 2+ and Co 2+ could improve the enzyme activity, while Cu 2+ , Hg 2+ , Fe 3+ and Al 3+ decreased the activity. The enzyme was inhibited by ethylenediaminetetraacetic acid (EDTA), ethylenebis(oxonitrilo)]tetra-acetate (EGTA), SDS and dimethyl sulfoxide (DMSO), but was not affected by phenylmethane-sulfonyl fluoride (PMSF) and 1,4-dithiothreitol (DTT). K m and V max values of the purified enzyme for soluble starch were 1.73 ± 0.3 mg/ml and 1.24 ± 0.02 mg/ml/min, respectively. The degradation ability of wild type α-amylase on starch granules was examined by thin layer chromatography. The final purified enzyme had an isoelectric point of 7.5-7.8 and α-helix of 28%, β-sheet of 32% and random coil of 40%.

IOP Conference Series: Materials Science and Engineering, 2021

Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants an... more Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants and vegetable storability. This study aims to determine the selenium content of baglog waste in increasing antioxidants and broccoli storability. The study was conducted from May to July 2019 in Cipadung Village, Cibiru District, Bandung using the Completely Randomized Design (CRD) consisted of 5 treatments and 5 replications. The treatment was media A = 100% baglog waste, B = baglog waste mixture 25%: 75% rice husk charcoal, C = Baglog waste mixture 50%: 50% rice husk charcoal, D = Baglog waste mixture 75%: 25% rice husk charcoal, E = 100% rice husk charcoal (control). The results showed that the combination of 75% baglog waste media with 25% rice husk charcoal was able to increase antioxidants by IC50 28.34 ppm and storability for four days at room temperature. 75% baglog waste with 25% husk charcoal can be used as a medium for supplying selenium to get good antioxidants and storability ...

Teknotan: Jurnal Industri Teknologi, Apr 15, 2022

Biji nangka dan biji durian merupakan limbah organik yang belum dimanfaatkan secara optimal. Biji... more Biji nangka dan biji durian merupakan limbah organik yang belum dimanfaatkan secara optimal. Biji durian mempunyai kandungan serat sebesar 22,48 % sedangkan biji nangka 7,46 %, yang dapat dimanfaatkan sebagai pangan fungsional. Senyawa oligosakarida yang terdapat dalam kedua biji tersebut dapat dijadikan sebagai sumber prebiotik pada bakteri asam laktat (BAL). Tujuan dari penelitian ini adalah untuk menganalisis efektivitas tepung biji nangka dan durian sebagai media pertumbuhan bakteri Lactobacillus casei. Pengujian hasil fermentasi tepung dilakukan pada jam ke 0, 24, 48, dan 72 dengan dilakukan analisis jumlah bakteri, pH, total asam, dan gula reduksi. Fermentasi 48 jam merupakan waktu puncak untuk pertumbuhan Lactobacillus casei. Jumlah bakteri pada tepung biji nangka sebanyak 3,24×10 10 sel/ml, sedangkan pada tepung biji durian 3,04 × 10 10 sel/ml. Nilai pH dari tepung biji nangka yaitu pH 3,8, sedangkan tepung biji durian 4,3. Total asam pada substrat tepung biji nangka yaitu 0,357 % dan 0,354 % pada tepung biji durian. Kadar gula reduksi pada tepung biji nangka 2,4658 mg/ml, sedangkan tepung biji durian 2,4925 mg/ml. Dengan membandingkan jumlah bakteri, nilai pH dan total asam diketahui bahwa tepung biji nangka lebih efektif sebagai prebiotik bagi bakteri L. casei daripada tepung biji durian.

IOP conference series, Mar 1, 2021

Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the a... more Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the application of production inputs should be directed to improve of those crop quality. The aim of the research was to know the effect of potassium concentration and type of planting media on production and quality of tomato (Lycopersicon esculentum) on hydroponic drip system. The research was conducted from June to August 2019 at Cileunyi Kulon village, Cilenyi district, Bandung regency, using split-plot design with 2 factors and 3 replications. Main plot was potassium concentration (350 ppm, 400 ppm, and 450 ppm) and sub-plot type of planting media (100 % husk charcoal, 100 % cocopeat, 50 % husk charcoal + 50 % cocopeat). The result showed that there was no. interaction effect of potassium concentration and type of planting media on fruit fresh weight and fruit hardness. The potassium application up to 450 ppm as well as the rice husk charcoal and cocopeat media produced the same effect on the fresh weight and the hardness of the fruit of tomatoes and its value were slightly below its potential in plant description. There are also the vitamin C levels obtained is in the low range of the vitamin C level.

Nucleation and Atmospheric Aerosols, 2023

The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps an... more The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps and the purity of enzymes was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results showed that, the enzyme was purified 4.7-fold with a specific activity of 134.6 U/mg protein and a yield of 44%. When it was subjected to SDS-PAGE, a single band near 58 kDa appeared. The optimum temperature and pH were 50°C and 7.0, respectively. The addition of Ca 2+ , Mn 2+ and Co 2+ could improve the enzyme activity, while Cu 2+ , Hg 2+ , Fe 3+ and Al 3+ decreased the activity. The enzyme was inhibited by ethylenediaminetetraacetic acid (EDTA), ethylenebis(oxonitrilo)]tetra-acetate (EGTA), SDS and dimethyl sulfoxide (DMSO), but was not affected by phenylmethane-sulfonyl fluoride (PMSF) and 1,4-dithiothreitol (DTT). K m and V max values of the purified enzyme for soluble starch were 1.73 ± 0.3 mg/ml and 1.24 ± 0.02 mg/ml/min, respectively. The degradation ability of wild type α-amylase on starch granules was examined by thin layer chromatography. The final purified enzyme had an isoelectric point of 7.5-7.8 and α-helix of 28%, β-sheet of 32% and random coil of 40%.

IOP conference series, Mar 1, 2021

Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants an... more Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants and vegetable storability. This study aims to determine the selenium content of baglog waste in increasing antioxidants and broccoli storability. The study was conducted from May to July 2019 in Cipadung Village, Cibiru District, Bandung using the Completely Randomized Design (CRD) consisted of 5 treatments and 5 replications. The treatment was media A = 100% baglog waste, B = baglog waste mixture 25%: 75% rice husk charcoal, C = Baglog waste mixture 50%: 50% rice husk charcoal, D = Baglog waste mixture 75%: 25% rice husk charcoal, E = 100% rice husk charcoal (control). The results showed that the combination of 75% baglog waste media with 25% rice husk charcoal was able to increase antioxidants by IC50 28.34 ppm and storability for four days at room temperature. 75% baglog waste with 25% husk charcoal can be used as a medium for supplying selenium to get good antioxidants and storability of broccoli.

Nucleation and Atmospheric Aerosols, 2023

Nucleation and Atmospheric Aerosols, 2023

Journal of physics, Dec 1, 2019

The impressed current cathodic protection is one of the effort to prevent a metal from corrosion ... more The impressed current cathodic protection is one of the effort to prevent a metal from corrosion by supplying electricity to metal objects. However, the protection system that supplies electricity must always be monitored so that the electricity intake to the metal object is sufficient to protect it from the corrosion. The way how to monitor the electricity intake to the metal is to read the current in taking on the metal. To read the electric current more easily is to use an application software. This research talks about the application design to display the electric current in taking on the metal through the computer screen. In order to read the electric current directly, the Advantect Data Acquisition Modul (ADAM) devices are used. The results show that the application is able to display the value of protection current for iron plate steel with size 30x10 cm are between 0.15 A to 0.18 A while for iron plate with a size of 30x13 cm are between 0.20 to 0.24 A. These values also avoid the over protection.

Materials Science Forum, Apr 1, 2021

Duration of use and injectability are external factors for Polydimethylsiloxane (PDMS) that needs... more Duration of use and injectability are external factors for Polydimethylsiloxane (PDMS) that needs to be considered when PDMS utilized as a vitreous substituted liquid in vitreoretinal surgery. In general, PDMS which has been used as a substitute for vitreous humour is PDMS with a low viscosity in the value about 1000 cSt and a high viscosity at a value of about 5000 cSt. Various deficiencies have been encountered from low and high viscosity of PDMS, causing research to be continued to obtain PDMS which has suitable properties as a substitute for vitreous humour. One of them is research to obtain medium viscosity of PDMS with a viscosity value of about 2000 cSt. Here, we reported synthesis and characterization of PDMS with medium viscosity in ranges from 1800 to 2600 mPas. PDMS was synthesized through Ring-Opening Polymerization (ROP) pathway using the monomer of Octamethylcyclotetrasiloxane (D4) and the chain terminator of Hexamethyldisiloxane (MM). Various concentrations of potassium hydroxide (KOH) of 3, 4, 6 and 8 %(w/v) were applied as initiator to form gel of PDMS. All synthesized PDMS samples were identified to have viscosity values of 1800-2600 mPas, refractive index values of 1.4042-1.4044 and surface tension values of 22-23 mN/m. Meanwhile, the results of Fourier-Transform Infrared (FTIR) measurement showed that the absorption peaks were similar to that of our previous report.

Journal of physics, Apr 1, 2021

The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is ... more The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is contained several secondary metabolites such as olive oil, phenolic compounds, flavonoids, triterpen, biphenol. The aims of this research are to isolate and identify a flavonoid compound that extracted in the ethyl acetate of olive leaves. Isolation was carried out by meseration, liquid vacuum chromatography, column chromatography and thin layer chromatography. Isolation results obtained 10 mg isolates were identified using proton nuclear magnetic spectroscopy (1H-NMR) and carbon (13C-NMR). According to spectra analysis, describe that isolate is chrysoeriol.

PROCEEDINGS OF THE SYMPOSIUM ON ADVANCE OF SUSTAINABLE ENGINEERING 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research

PROCEEDINGS OF THE SYMPOSIUM ON ADVANCE OF SUSTAINABLE ENGINEERING 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research

Jurnal Informatika, Jun 10, 2020

Gunung Djati Conference Series, Jul 1, 2021

IOP Conference Series: Materials Science and Engineering, 2021

Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the a... more Tomato is a crop known as nutritious due to its protein, mineral, and vitamins contains. So the application of production inputs should be directed to improve of those crop quality. The aim of the research was to know the effect of potassium concentration and type of planting media on production and quality of tomato (Lycopersicon esculentum) on hydroponic drip system. The research was conducted from June to August 2019 at Cileunyi Kulon village, Cilenyi district, Bandung regency, using split-plot design with 2 factors and 3 replications. Main plot was potassium concentration (350 ppm, 400 ppm, and 450 ppm) and sub-plot type of planting media (100 % husk charcoal, 100 % cocopeat, 50 % husk charcoal + 50 % cocopeat). The result showed that there was no. interaction effect of potassium concentration and type of planting media on fruit fresh weight and fruit hardness. The potassium application up to 450 ppm as well as the rice husk charcoal and cocopeat media produced the same effect o...

Journal of Physics: Conference Series, 2021

The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is ... more The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is contained several secondary metabolites such as olive oil, phenolic compounds, flavonoids, triterpen, biphenol. The aims of this research are to isolate and identify a flavonoid compound that extracted in the ethyl acetate of olive leaves. Isolation was carried out by meseration, liquid vacuum chromatography, column chromatography and thin layer chromatography. Isolation results obtained 10 mg isolates were identified using proton nuclear magnetic spectroscopy (1H-NMR) and carbon (13C-NMR). According to spectra analysis, describe that isolate is chrysoeriol.

Journal of Physics: Conference Series, 2019

Internal corrosion in the piping system is a serious problem. The use of corrosion inhibitors for... more Internal corrosion in the piping system is a serious problem. The use of corrosion inhibitors for corrosion control in piping systems is a practical and cost-effective method. Many polymers have been reported to be used as an inhibitor corrosion. However, there was little attention to the research of Polyethylene Oxide (PEO) as a corrosion inhibitor in NaCl medium. The purpose of this study was to determine the corrosion inhibition activity of polymeric compounds of polyethylene oxide against ferrous metals in 1% NaCl electrolyte solution. The study of Polyethylene Oxide (PEO) activity as a corrosion inhibitor in 1% NaCl electrolyte solution was carried out by the Wheel Test method with variations in concentration and temperature. The surface analysis of iron by SEM (Scanning Electron Microscopy). The results showed that polyethylene oxide (PEO) had the optimum inhibitory activity at a concentration of 16 ppm, with inhibition efficiency of 80.15% at 25°C. Polyethylene Oxide has adso...

IOP Conference Series: Earth and Environmental Science, 2019

Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been ... more Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been known and used as medicines. Exploration on torch ginger plant needs to be done to conservate and determine genetic diversity. Study of genetic diversity torch ginger in the district of Pangandaran is important because as long as there has been no study in depth the potential of genetic diversity and phylogenetic relationship germplasm of torch ginger Genetic diversity based on this research had a proportion of 47% of the total of 100% in the PC1-PC3 was based on the analysis Principal Component Analysis (PCA) with the character influencing that form clumps, line leaf midrib, plant height, stem diameter, leaf length, color scales rhizome, stem color, rhizome and the leaf base forms. Based on the results of the genetic diversity cluster analysis found that narrow torch ginger euclidean indicated by coefficient of 0.79 and was divided into six clusters. Accession which had the closest kins...

IOP Conference Series: Materials Science and Engineering, 2018

Graphite is a natural carbon source that can be used for graphene synthesis because of its abunda... more Graphite is a natural carbon source that can be used for graphene synthesis because of its abundant availability and relatively low cost. In this research, Graphene Oxide (GO) and reduced Graphene Oxide (rGO) have been prepared through modified Hummers method that uses microwave irradiation and hydrothermal process as reduction methods. This research can be divided into two main steps, i.e. preparation of GO and preparation of rGO. The preparation of GO was done using modified Hummers method that refers to graphite powder oxidation for five days. Then, preparation of rGO was done using two methods, i.e. microwave irradiation for 8 minutes at 1,000 watts and hydrothermal process for 10 hours at 200°C. The characterization of the samples was observed by Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Ultraviolet-Visible Spectroscopy (UV-Vis). XRD pattern of GO sample shows a peak at 2θ = 10.63°, rGO sample from hydrothermal process at 2θ = 25.94°, and rGO sample from microwave irradiation method at 2θ = 25.94° with low intensity and at 2θ = 10.63° that indicates the presence of GO. IR spectroscopy data shows an absorption peak of aromatic C=C at 1,573 cm-1 that indicates rGO formation. SEM images of GO illustrates a structure of stacked flakes. While the morphology of rGO has a structure of stacked flakes that is more transparent than GO. According to UV-Vis spectroscopy, GO and rGO of samples show absorption peak at λmax = 225 nm and λmax = 274 nm, respectively, which indicates π→π* transition of aromatic C=C bond. Based on the results of this study, the preparation of rGO using modified Hummers that combined with hydrothermal reduction method is more effective than microwave irradiation reduction method.

African Journal of Biotechnology, 2011

The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps an... more The α-amylase of marine Pseudomonas sp. K6-28-040 was purified through a series of three steps and the purity of enzymes was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results showed that, the enzyme was purified 4.7-fold with a specific activity of 134.6 U/mg protein and a yield of 44%. When it was subjected to SDS-PAGE, a single band near 58 kDa appeared. The optimum temperature and pH were 50°C and 7.0, respectively. The addition of Ca 2+ , Mn 2+ and Co 2+ could improve the enzyme activity, while Cu 2+ , Hg 2+ , Fe 3+ and Al 3+ decreased the activity. The enzyme was inhibited by ethylenediaminetetraacetic acid (EDTA), ethylenebis(oxonitrilo)]tetra-acetate (EGTA), SDS and dimethyl sulfoxide (DMSO), but was not affected by phenylmethane-sulfonyl fluoride (PMSF) and 1,4-dithiothreitol (DTT). K m and V max values of the purified enzyme for soluble starch were 1.73 ± 0.3 mg/ml and 1.24 ± 0.02 mg/ml/min, respectively. The degradation ability of wild type α-amylase on starch granules was examined by thin layer chromatography. The final purified enzyme had an isoelectric point of 7.5-7.8 and α-helix of 28%, β-sheet of 32% and random coil of 40%.

IOP Conference Series: Materials Science and Engineering, 2021

Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants an... more Baglog waste contains cellulose, which can absorb selenium. Selenium can increase antioxidants and vegetable storability. This study aims to determine the selenium content of baglog waste in increasing antioxidants and broccoli storability. The study was conducted from May to July 2019 in Cipadung Village, Cibiru District, Bandung using the Completely Randomized Design (CRD) consisted of 5 treatments and 5 replications. The treatment was media A = 100% baglog waste, B = baglog waste mixture 25%: 75% rice husk charcoal, C = Baglog waste mixture 50%: 50% rice husk charcoal, D = Baglog waste mixture 75%: 25% rice husk charcoal, E = 100% rice husk charcoal (control). The results showed that the combination of 75% baglog waste media with 25% rice husk charcoal was able to increase antioxidants by IC50 28.34 ppm and storability for four days at room temperature. 75% baglog waste with 25% husk charcoal can be used as a medium for supplying selenium to get good antioxidants and storability ...