Selahattin Aydemir - Academia.edu (original) (raw)

Papers by Selahattin Aydemir

Research paper thumbnail of Genetic Diversity of <i>Echinococcus granulosus</i> Isolated from Humans: A Comparative Study in Two Cystic Echinococcosis Endemic Areas, Turkey and Iran

BioMed Research International, Apr 28, 2020

Cystic echinococcosis (CE) is one of the most important zoonotic parasitic diseases caused by the... more Cystic echinococcosis (CE) is one of the most important zoonotic parasitic diseases caused by the larval stage of Echinococcus granulosus. Based on molecular studies and DNA sequencing, E. granulosus has been classified into 10 different genotypes (G1 to G10). Two neighboring countries, Turkey and Iran, are considered the two main foci of CE in the Middle East. The current study is aimed at examining the genotype diversity of E. granulosus isolated from human clinical samples in Turkey and Iran. Surgically removed human hydatid cysts were collected from East Azerbaijan and Fars provinces in Iran and Van province in Turkey. After extracting DNA, performing PCR, targeting the cox1 gene, the PCR products were purified from the gel and were sequenced from both directions. The sequences were aligned and compared, using BioEdit and also the BLAST program of GenBank. The maximum likelihood tree was constructed based on the Tamura-Nei model, using the MEGAX software. Phylogenetic analysis showed that the human isolated samples were classified into two major clades: G1 (from Iran and Turkey) and G3 (5 samples from northwestern Iran and one sample from Turkey). The mean and degree of genetic divergence (K2P) between the two major clades, G1 and G3, were 0.2% and 0:7 ± 0:4%, respectively. The findings of the current study revealed that the sheep strain (G1) and the less important strain G3 have major roles in the transmission cycle of CE in two neighboring countries, Iran and Turkey. Therefore, it is necessary to interpose the life cycle of this parasite and reduce the disease burden in livestock and humans by adopting common regional preventive and control policies.

Research paper thumbnail of Relationship of Demodex Mites in Immunodeficiency, Rocesea, Blepharitis and Some Clinical Findings

Middle black sea journal of health science, Nov 30, 2022

Objective: Demodex folliculorum and Demodex brevis are two species known to settle on the skin of... more Objective: Demodex folliculorum and Demodex brevis are two species known to settle on the skin of humans. Demodex mite infections are called demodicosis. Demodicosis, which is usually asymptomatic, is known to cause some skin diseases as a result of an imbalance in immune system mechanisms This study was conducted to investigate the relationship between Demodex spp. infestations and clinical signs, such as immunodeficiency, rosacea, blepharitis and facial itching, facial flushing, facial tenderness, facial rash, and sunburn. Methods: A total of 350 patients, 178 of whom were immunosuppressed and 172 who were immunocompetent, were included in the study. Samples were taken from the nose, chin, and forehead areas, using the standard superficial skin biopsy method and were examined under a microscope. Results: Demodex spp. was detected in 224 of the 350 patients, including 144 (80.90%) of the 178 immunosuppressed patients and 88 (51.16%) of the 172 immunocompetent patients included in the study. The difference between Demodex spp. positivity in the immunosuppressed patients and positivity in the immunocompetent patients was statistically significant. In addition, a relationship was found between Demodex spp. and some clinical symptoms. Conclusion: Demodex spp. proceed a health problem in rosacea and immunosuppressed patients. It was concluded that Demodex spp. should definitely be considered in cases of facial sensitivity, facial rash, and facial flushing in both immunosuppressed and immunocompetent patients, especially in the presence of sunburn in immunosuppressed patients, which was revealed by this study.

Research paper thumbnail of Gastrointestinal Helminths and Zoonotic Importance Detected in Stray Dogs in Ağrı Province and Districts

Türkiye parazitoloji dergisi, Mar 1, 2022

Bu çalışma; Ağrı ili ve ilçelerinde sokak köpeklerinde bulunan gastrointestinal helmint varlığını... more Bu çalışma; Ağrı ili ve ilçelerinde sokak köpeklerinde bulunan gastrointestinal helmint varlığını araştırmak ve bu parazitlerin zoonotik önemini ortaya koymak amacıyla yapılmıştır.

Research paper thumbnail of Synthesis and biological properties of novel 1-methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene) hydrazine analogues

Turkish Journal of Chemistry, Apr 27, 2018

1-Methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene)hydrazine analogues were readily prepared in good yi... more 1-Methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene)hydrazine analogues were readily prepared in good yields by the reaction of 2-(prop-2-yn-1-yloxy)benzaldehydes and methyl hydrazine. The reaction tolerates a variety of substituents on the 2-hydroxybenzaldehyde to form nitro-, halo-, methoxy-, and naphthyl-substituted 1-methyl-2-(2-(prop-2-yn-1yloxy)benzylidene)hydrazines. The in vitro antioxidant capacity measurements revealed that among all the analyzed hydrazine analogues that surpassed the Trolox standard, 1-(2-(but-3-ynyl)-5-nitrobenzylidene)-2-methylhydrazine had the maximum value, which was approximately 1.7 times that of Trolox.

Research paper thumbnail of Investigation of the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis in immunosuppressed patients with diarrhea by IFA and real time PCR methods

Indian Journal of Medical Microbiology, Jul 1, 2023

Research paper thumbnail of Molecular Prevalence of Larval Stages of <i>Fasciola hepatica</i> in <i>Lymnaea stagnalis</i> Species Snails in the Vicinity of the Ağrı Province

Türkiye parazitoloji dergisi, Mar 2, 2023

Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fascio... more Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fasciola hepatica'nın ara konaklarından birisidir. Bu çalışmada Ağrı ili civarında toplanan L. stagnalis türü salyangozlarda F. hepatica'nın larval formlarının polimeraz zincir reaksiyonu (PZR) ile belirlenmesi amaçlanmıştır. Yöntemler: Bu çalışmada Ağrı ilinden toplam 150 adet L. stagnalis türü salyangoz toplanmıştır. Laboratuvara getirilen tatlı su salyangozları diseke edildikten sonra yumuşak dokuları mikroskop altında incelenmiştir. Diseke edilen salyangozlara DNA ekstraksiyonu işlemi yapılmıştır. DNA ekstraksiyonu sonrasında sitokrom c oksidaz alt birimi 1 gen bölgesini hedefleyen primerler kullanılarak PZR işlemi gerçekleştirilmiştir. Bulgular: Mikroskobik bakıda F. hepatica'nın larval formları tespit edilememiştir. Ancak PZR işlemi sonrasında yapılan analizde iki (%1,3) L. stagnalis tatlı su salyangozunun F. hepatica'nın larval formlarıyla enfekte olduğu sonucuna ulaşılmıştır.

Research paper thumbnail of The Importance of Antioxidant Enzymes and Oxidative Stress in Human Fascioliasis

Türkiye parazitoloji dergisi, Mar 2, 2023

The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione pero... more The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in the patients infected with Fasciola hepatica and establish whether these parameters differ among the patients with fascioliasis. Methods: The patient group consisted of 140 individuals with F. hepatica seropositive; the control group consisted of 140 healthy individuals who tested negative for this parasite and had no other diseases. The patient group consisted of individuals with no chronic diseases other than fascioliasis; in both the patient and the control groups, the subjects had no unhealthy habits such as smoking and alcohol consumption, etc. The blood samples taken to diagnose fascioliasis were evaluated by the ELISA method. The samples were studied according to the kit procedures for SOD, CAT, GPx and MDA markers. Results: In this study, 43.6% of 140 individuals in the patient group infected with F. hepatica had CAT (p=0.001), 35% had GPx (p=0.001), 12.9% had SOD (p=0.002), 90.7% had MDA (p=0.001). There was found a statistically significant difference between the patient and the control group in terms of the positivity of these four parameters. Conclusion: As a result, a statistically significant relationship was found between the increase in the SOD, GPx, CAT, and MDA levels and fascioliasis. The high rate of MDA revealed that oxidative stress occurred in patients with fascioliasis, resulting in an increased activity of SOD, GPx, and CAT.

Research paper thumbnail of The Impact of COVID-19 Pandemic on Intestinal Parasite Frequency: A Retrospective Study

Eastern Journal Of Medicine

Research paper thumbnail of Frequency of Intestinal Parasites in Individuals with Immunosuppression and Certain Chronic Diseases

Van Medical Journal

Paraziter etkenler, hastanın bağışıklık durumuna bakılmaksızın tüm dünyada önemli morbidite ve mo... more Paraziter etkenler, hastanın bağışıklık durumuna bakılmaksızın tüm dünyada önemli morbidite ve mortalite sebebidirler. İnsanları enfekte edebilen yaklaşık 340 parazit türünün olduğu tahmin edilmektedir. En çok enfeksiyona sebep olan türler ise bağırsak parazitleridir. Dünya genelinde iki milyara yakın insanın bağırsak parazitlerinden etkilendiği ve yaklaşık 300 milyon kişide ciddi semptomların oluştuğu tahmin edilmektedir. Bağırsak parazitleri özellikle immünsüprese hastalarda malnütrisyon, anemi, gelişim geriliği ve zihinsel bozukluk gibi bulgulardan akut komplikasyonlara karşı artan duyarlılığa kadar farklı klinik semptomlar gösterebilmektedir (1). Çeşitli ülkelerde epidemiyoloji ile ilgili yapılan çalışmalar, sosyo-ekonomik düzeyin intestinal parazitlerin prevalansını etkileyebildiğini

Research paper thumbnail of Investigation of Blastocystis hominis Frequency in Patients with Diabetes by Microscopy and Conventional PCR Methods

Iranian Journal of Parasitology

Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. M... more Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. Methods: One hundred and fifty diabetic patients and 100 healthy people without any chronic disease were included in the study. Stool samples were analyzed by native-Lugol, condensation, trichrome staining and PCR methods. Results: İn 150 patients with diabetes; B. hominis was detected in 38 (25.3%) by PCR, in 34 (22.7%) by native-Lugol and trichrome staining. In the control group, 14 (14%) out of 100 subjects were positive by PCR, and 10 (10%) were positive by native-Lugol and trichrome staining. In the statistical evaluation, a significant difference was found between gender (P=0.023), age (P=0.045; ≤35 and >35 comparison), duration of diabetes (P=0.04), the HbA1c value (P=0.023; <8 and ≥8 comparison), and B. hominis positivity. ST1 was determined in 76.9% of patients with diabetes, and ST2 was determined in 23.07%. Considering the 3 methods, B. hominis positivity was detected in ...

Research paper thumbnail of Distribution of Toxocara vitulorum in Cattle of Ağrı Region

Turkish Journal of Parasitology

Amaç: Sığır, manda ve bizonların ince bağırsaklarında parazitlenen Toxocara vitulorum, Türkiye da... more Amaç: Sığır, manda ve bizonların ince bağırsaklarında parazitlenen Toxocara vitulorum, Türkiye dahil, nemli iklime sahip tropikal ve subtropikal iklim kuşağında bulunan ülkelerde görülmektedir. Erişkin parazitler özellikle buzağılarda iştahsızlık, zayıflama, ishal veya kabızlık gibi sindirim bozukluklarına ve bazen ölümlere neden olabilmektedir. Bu çalışma, Ağrı yöresi sığırlarında T. vitulorum'un yayılışını araştırmak amacıyla yapıldı. Yöntemler: Bu çalışmada, Ağrı ili ve ilçelerinde 0-6 aylık 200 buzağı ve 1 yaştan büyük 200 sığır rektumundan bir ceviz büyüklüğünde dışkı örneği alınarak, doymuş çinko sülfat flotasyon yöntemi ile preparatlar hazırlandı ve preparatlar ışık mikroskobunda incelendi. Bulgular: Dışkı örnekleri incelenen 0-6 aylık 200 buzağının 70'inde (%35), bir yaş üstü 200 sığırın 21'inde (%10,5) T. vitulorum yumurtası saptandı. Bir yaş üstü sığırlar ile buzağılar arasındaki pozitiflik farkı istatistiksel olarak anlamlı bulundu (p=0,001). Dışkı bakısı yapılan buzağıların 22'sinde (%11) ise Trichostrongylidae spp. yumurtaları saptandı.

Research paper thumbnail of Investigation of Giardia spp., Cryptosporidium spp. and Cyclospora cayetanensis in Samples Collected from Different Spring Waters Iğdır, Türkiye

Turkish Journal of Parasitology

In this study, it was aimed to investigate the physical and chemical properties of different spri... more In this study, it was aimed to investigate the physical and chemical properties of different spring waters and parasitic factors with different methods. Methods: This study was carried out on 69 water samples collected from different spring waters in and around Iğdır Province in April and June 2021. The samples were analyzed by native-Lugol, modified acid-fast staining, and nested polymerase chain reaction (nPCR). In addition, altitude (meter) and pressure (mmHg) measurements were made at the point where the water samples were taken. Results: One or more parasites were detected in 27.5% of the 69 water samples examined. Only C. cayetanensis was found in 13% of the samples, only Cryptosporidium spp. in 10.1%, only Giardia spp. in 1.4%, only C. cayetanensis and Giardia spp. in 1.4%, only C. cayetanensis, Cryptosporidium spp., and Giardia spp. in 1.4%. Only Giardia spp. cyst (4.3%) was detected by the direct examination method. While C. cayetanensis and Cryptosporidium spp. oocysts were detected in 8.7% and 7.2% of the samples by the modified acid-fast staining method, C. cayetanensis was detected in 15.9% and Cryptosporidium spp. was detected in 11.6% of the samples by nPCR. When the C. cayetanensis and Cryptosporidium spp. positivity rates were compared according to the characteristics of the water, there was no statistical difference between the altitude, salinity, pH, mmHg, and temperature (kelvin) values, but a significant correlation was found between the amount of dissolved oxygen and Cryptosporidium spp. positivity (p=0.047). Conclusion: Cryptosporidium spp., C. cayetanensis, and G. intestinalis are important waterborne pathogens that can cause epidemics. It is our belief that in order to reduce the risk of contamination of these parasitic factors with spring waters, public awareness should be raised, infrastructures should be improved, and new water treatment techniques, such as ultraviolet, ozonation and monitoring systems, should be used.

Research paper thumbnail of Global Trends on Blastocystis sp. Research: A Scientometric Study

Parasitologists United Journal

Research paper thumbnail of Sağlık Hizmetleri Meslek Yüksekokulu Öğrencilerinin Paraziter Hastalıklar Hakkındaki Bilgi Düzeyleri

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 2021

In this study, it has been aimed to determine the knowledge level of the students of Van Yuzuncu ... more In this study, it has been aimed to determine the knowledge level of the students of Van Yuzuncu Yıl University Health Services Vocational School, who will serve as the health personnel of the future, about parasitic diseases, prevention and control measures from these diseases. A total of 587 students, comprising 418 (71.2%) females and 169 (28.8%) males, studying at Van Yüzüncü Yıl University Health Services Vocational School, were included in the study. The questions in the questionnaire were arranged in three groups, as those about parasitic diseases, transmission routes of these diseases, and prevention and treatment. Cronbach alpha coefficients were calculated within the scope of the reliability analysis of the questionnaire questions. The Cronbach alpha values for the domains of Knowledge Level About Parasitic Diseases, Knowledge Level About the Transmission of Parasitic Diseases, and Knowledge Level About the Prevention and Treatment of Parasitic Diseases were determined as ...

Research paper thumbnail of Investigation of the Effect of Pasteurization on the Viability of Cryptosporidium parvum in Cow’s Milk by Propidium Monoazide qPCR

Research paper thumbnail of Investigation of the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis in immunosuppressed patients with diarrhea by IFA and real time PCR methods

Indian Journal of Medical Microbiology

Research paper thumbnail of Molecular Prevalence of Larval Stages of Fasciola hepatica in Lymnaea stagnalis Species Snails in the Vicinity of the Ağrı Province

Turkish Journal of Parasitology

Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fascio... more Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fasciola hepatica'nın ara konaklarından birisidir. Bu çalışmada Ağrı ili civarında toplanan L. stagnalis türü salyangozlarda F. hepatica'nın larval formlarının polimeraz zincir reaksiyonu (PZR) ile belirlenmesi amaçlanmıştır. Yöntemler: Bu çalışmada Ağrı ilinden toplam 150 adet L. stagnalis türü salyangoz toplanmıştır. Laboratuvara getirilen tatlı su salyangozları diseke edildikten sonra yumuşak dokuları mikroskop altında incelenmiştir. Diseke edilen salyangozlara DNA ekstraksiyonu işlemi yapılmıştır. DNA ekstraksiyonu sonrasında sitokrom c oksidaz alt birimi 1 gen bölgesini hedefleyen primerler kullanılarak PZR işlemi gerçekleştirilmiştir. Bulgular: Mikroskobik bakıda F. hepatica'nın larval formları tespit edilememiştir. Ancak PZR işlemi sonrasında yapılan analizde iki (%1,3) L. stagnalis tatlı su salyangozunun F. hepatica'nın larval formlarıyla enfekte olduğu sonucuna ulaşılmıştır.

Research paper thumbnail of Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method

Turkish Journal of Parasitology

Pneumocystis jirovecii'nin Nested PZR Yöntemi ile Araştırılması Objective: Pneumocystis jirovecii... more Pneumocystis jirovecii'nin Nested PZR Yöntemi ile Araştırılması Objective: Pneumocystis jirovecii (P. jirovecii) is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with P. jirovecii pneumonia (PJP) remains a key priority. This study investigated P. jirovecii in patients with lung cancer using the nested-polymerase chain reaction (PCR) method and examined the relationship between P. jirovecii and clinical findings. Methods: The study included 60 patients with lung cancer and 30 patients without lung cancer. The bronchoalveolar lavage (BAL) fluid samples of these 90 individuals were taken for diagnostic purposes in the University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Chest Diseases. Patient information was recorded. After DNA isolation from the BAL fluid samples taken from patients, the nested-PCR protocol for amplification of mtLSUrRNA in P. jirovecii was performed. Results: P. jirovecii DNA was detected in 40 (66.67%) of the lung cancer patients included in the study and in six (20%) patients without lung cancer, that is, in 46 (51.11%) patients. The rate of nested-PCR positivity in the lung cancer group was significantly higher than that in the non-lung cancer group (p=0.0001). Additionally, a statistically significant correlation was found between anorexia and weight loss, fever and sputum P. jirovecii positivity in patients with lung cancer (p<0.005). Conclusion: These findings suggest that lung cancer patients should be evaluated for PJP.

Research paper thumbnail of Global Trends on Blastocystis sp. Research: A Scientometric Study

Blastocystis sp. is the most common unicellular, luminal parasite in humans and animals. It has b... more Blastocystis sp. is the most common unicellular, luminal parasite in humans and animals. It has been associated with diarrhea and irritable bowel syndrome. This article describes the bibliometric research of articles published in Web of Science (WoS) database on Blastocystis sp. over a 51-year period. The VOSviewer visualization methodology was used for this study. Institutions, nations, international collaborations, journals, articles, authors, keywords, co-authors, co-citations, and citation rates from the WoS database formed the data of this study. A total of 1066 documents were found in the WoS database. Of which 773 were articles and only those publications were analyzed. The articles were cited 22,034 times in total and the mean Hirsch (H) index was 74. The countries with the highest number of articles were as follows: USA (n = 86; 11.125%), Singapore (n = 71; 9.185%), and Malaysia (n = 70; 9.056). Since 2000, there have been a limited number of articles and since 2014 there h...

Research paper thumbnail of Subtyping of Cryptosporidium parvum Obtained from Humans and Calves in Van, Turkey

Iranian Journal of Parasitology

Background: We aimed to investigate the prevalence of Cryptosporidium species detected in humans ... more Background: We aimed to investigate the prevalence of Cryptosporidium species detected in humans and calves in the Van region of Turkey. Methods: A total of 150 patients, comprising 60 who were immunosuppressed, 50 who were immunosuppressed and had diarrhea, and 40 who had only diarrhea, were enrolled in this study in the Department of Medical Parasitology, Van Yuzuncu Yıl University Faculty of Medicine, Turkey. Stool samples were taken from the rectums of a total of 50 calves that had 30 diarrhea and 20 that did not have diarrhea, from the stables and farms of 10 central villages of Van, Turkey. All samples were analyzed using modified acid-fast staining, immunochromatographic test, and PCR. Cryptosporidium positive samples were also subtyped. Results: Only C. parvum subtypes were detected in all positive samples. C. parvum was detected in 30 (20%) of the 150 human stool samples, while it was detected in 5 (10%) of the 50 samples from the calves. The GP60 gene region was amplified ...

Research paper thumbnail of Genetic Diversity of <i>Echinococcus granulosus</i> Isolated from Humans: A Comparative Study in Two Cystic Echinococcosis Endemic Areas, Turkey and Iran

BioMed Research International, Apr 28, 2020

Cystic echinococcosis (CE) is one of the most important zoonotic parasitic diseases caused by the... more Cystic echinococcosis (CE) is one of the most important zoonotic parasitic diseases caused by the larval stage of Echinococcus granulosus. Based on molecular studies and DNA sequencing, E. granulosus has been classified into 10 different genotypes (G1 to G10). Two neighboring countries, Turkey and Iran, are considered the two main foci of CE in the Middle East. The current study is aimed at examining the genotype diversity of E. granulosus isolated from human clinical samples in Turkey and Iran. Surgically removed human hydatid cysts were collected from East Azerbaijan and Fars provinces in Iran and Van province in Turkey. After extracting DNA, performing PCR, targeting the cox1 gene, the PCR products were purified from the gel and were sequenced from both directions. The sequences were aligned and compared, using BioEdit and also the BLAST program of GenBank. The maximum likelihood tree was constructed based on the Tamura-Nei model, using the MEGAX software. Phylogenetic analysis showed that the human isolated samples were classified into two major clades: G1 (from Iran and Turkey) and G3 (5 samples from northwestern Iran and one sample from Turkey). The mean and degree of genetic divergence (K2P) between the two major clades, G1 and G3, were 0.2% and 0:7 ± 0:4%, respectively. The findings of the current study revealed that the sheep strain (G1) and the less important strain G3 have major roles in the transmission cycle of CE in two neighboring countries, Iran and Turkey. Therefore, it is necessary to interpose the life cycle of this parasite and reduce the disease burden in livestock and humans by adopting common regional preventive and control policies.

Research paper thumbnail of Relationship of Demodex Mites in Immunodeficiency, Rocesea, Blepharitis and Some Clinical Findings

Middle black sea journal of health science, Nov 30, 2022

Objective: Demodex folliculorum and Demodex brevis are two species known to settle on the skin of... more Objective: Demodex folliculorum and Demodex brevis are two species known to settle on the skin of humans. Demodex mite infections are called demodicosis. Demodicosis, which is usually asymptomatic, is known to cause some skin diseases as a result of an imbalance in immune system mechanisms This study was conducted to investigate the relationship between Demodex spp. infestations and clinical signs, such as immunodeficiency, rosacea, blepharitis and facial itching, facial flushing, facial tenderness, facial rash, and sunburn. Methods: A total of 350 patients, 178 of whom were immunosuppressed and 172 who were immunocompetent, were included in the study. Samples were taken from the nose, chin, and forehead areas, using the standard superficial skin biopsy method and were examined under a microscope. Results: Demodex spp. was detected in 224 of the 350 patients, including 144 (80.90%) of the 178 immunosuppressed patients and 88 (51.16%) of the 172 immunocompetent patients included in the study. The difference between Demodex spp. positivity in the immunosuppressed patients and positivity in the immunocompetent patients was statistically significant. In addition, a relationship was found between Demodex spp. and some clinical symptoms. Conclusion: Demodex spp. proceed a health problem in rosacea and immunosuppressed patients. It was concluded that Demodex spp. should definitely be considered in cases of facial sensitivity, facial rash, and facial flushing in both immunosuppressed and immunocompetent patients, especially in the presence of sunburn in immunosuppressed patients, which was revealed by this study.

Research paper thumbnail of Gastrointestinal Helminths and Zoonotic Importance Detected in Stray Dogs in Ağrı Province and Districts

Türkiye parazitoloji dergisi, Mar 1, 2022

Bu çalışma; Ağrı ili ve ilçelerinde sokak köpeklerinde bulunan gastrointestinal helmint varlığını... more Bu çalışma; Ağrı ili ve ilçelerinde sokak köpeklerinde bulunan gastrointestinal helmint varlığını araştırmak ve bu parazitlerin zoonotik önemini ortaya koymak amacıyla yapılmıştır.

Research paper thumbnail of Synthesis and biological properties of novel 1-methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene) hydrazine analogues

Turkish Journal of Chemistry, Apr 27, 2018

1-Methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene)hydrazine analogues were readily prepared in good yi... more 1-Methyl-2-(2-(prop-2-yn-1-yloxy)benzylidene)hydrazine analogues were readily prepared in good yields by the reaction of 2-(prop-2-yn-1-yloxy)benzaldehydes and methyl hydrazine. The reaction tolerates a variety of substituents on the 2-hydroxybenzaldehyde to form nitro-, halo-, methoxy-, and naphthyl-substituted 1-methyl-2-(2-(prop-2-yn-1yloxy)benzylidene)hydrazines. The in vitro antioxidant capacity measurements revealed that among all the analyzed hydrazine analogues that surpassed the Trolox standard, 1-(2-(but-3-ynyl)-5-nitrobenzylidene)-2-methylhydrazine had the maximum value, which was approximately 1.7 times that of Trolox.

Research paper thumbnail of Investigation of the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis in immunosuppressed patients with diarrhea by IFA and real time PCR methods

Indian Journal of Medical Microbiology, Jul 1, 2023

Research paper thumbnail of Molecular Prevalence of Larval Stages of <i>Fasciola hepatica</i> in <i>Lymnaea stagnalis</i> Species Snails in the Vicinity of the Ağrı Province

Türkiye parazitoloji dergisi, Mar 2, 2023

Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fascio... more Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fasciola hepatica'nın ara konaklarından birisidir. Bu çalışmada Ağrı ili civarında toplanan L. stagnalis türü salyangozlarda F. hepatica'nın larval formlarının polimeraz zincir reaksiyonu (PZR) ile belirlenmesi amaçlanmıştır. Yöntemler: Bu çalışmada Ağrı ilinden toplam 150 adet L. stagnalis türü salyangoz toplanmıştır. Laboratuvara getirilen tatlı su salyangozları diseke edildikten sonra yumuşak dokuları mikroskop altında incelenmiştir. Diseke edilen salyangozlara DNA ekstraksiyonu işlemi yapılmıştır. DNA ekstraksiyonu sonrasında sitokrom c oksidaz alt birimi 1 gen bölgesini hedefleyen primerler kullanılarak PZR işlemi gerçekleştirilmiştir. Bulgular: Mikroskobik bakıda F. hepatica'nın larval formları tespit edilememiştir. Ancak PZR işlemi sonrasında yapılan analizde iki (%1,3) L. stagnalis tatlı su salyangozunun F. hepatica'nın larval formlarıyla enfekte olduğu sonucuna ulaşılmıştır.

Research paper thumbnail of The Importance of Antioxidant Enzymes and Oxidative Stress in Human Fascioliasis

Türkiye parazitoloji dergisi, Mar 2, 2023

The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione pero... more The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in the patients infected with Fasciola hepatica and establish whether these parameters differ among the patients with fascioliasis. Methods: The patient group consisted of 140 individuals with F. hepatica seropositive; the control group consisted of 140 healthy individuals who tested negative for this parasite and had no other diseases. The patient group consisted of individuals with no chronic diseases other than fascioliasis; in both the patient and the control groups, the subjects had no unhealthy habits such as smoking and alcohol consumption, etc. The blood samples taken to diagnose fascioliasis were evaluated by the ELISA method. The samples were studied according to the kit procedures for SOD, CAT, GPx and MDA markers. Results: In this study, 43.6% of 140 individuals in the patient group infected with F. hepatica had CAT (p=0.001), 35% had GPx (p=0.001), 12.9% had SOD (p=0.002), 90.7% had MDA (p=0.001). There was found a statistically significant difference between the patient and the control group in terms of the positivity of these four parameters. Conclusion: As a result, a statistically significant relationship was found between the increase in the SOD, GPx, CAT, and MDA levels and fascioliasis. The high rate of MDA revealed that oxidative stress occurred in patients with fascioliasis, resulting in an increased activity of SOD, GPx, and CAT.

Research paper thumbnail of The Impact of COVID-19 Pandemic on Intestinal Parasite Frequency: A Retrospective Study

Eastern Journal Of Medicine

Research paper thumbnail of Frequency of Intestinal Parasites in Individuals with Immunosuppression and Certain Chronic Diseases

Van Medical Journal

Paraziter etkenler, hastanın bağışıklık durumuna bakılmaksızın tüm dünyada önemli morbidite ve mo... more Paraziter etkenler, hastanın bağışıklık durumuna bakılmaksızın tüm dünyada önemli morbidite ve mortalite sebebidirler. İnsanları enfekte edebilen yaklaşık 340 parazit türünün olduğu tahmin edilmektedir. En çok enfeksiyona sebep olan türler ise bağırsak parazitleridir. Dünya genelinde iki milyara yakın insanın bağırsak parazitlerinden etkilendiği ve yaklaşık 300 milyon kişide ciddi semptomların oluştuğu tahmin edilmektedir. Bağırsak parazitleri özellikle immünsüprese hastalarda malnütrisyon, anemi, gelişim geriliği ve zihinsel bozukluk gibi bulgulardan akut komplikasyonlara karşı artan duyarlılığa kadar farklı klinik semptomlar gösterebilmektedir (1). Çeşitli ülkelerde epidemiyoloji ile ilgili yapılan çalışmalar, sosyo-ekonomik düzeyin intestinal parazitlerin prevalansını etkileyebildiğini

Research paper thumbnail of Investigation of Blastocystis hominis Frequency in Patients with Diabetes by Microscopy and Conventional PCR Methods

Iranian Journal of Parasitology

Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. M... more Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. Methods: One hundred and fifty diabetic patients and 100 healthy people without any chronic disease were included in the study. Stool samples were analyzed by native-Lugol, condensation, trichrome staining and PCR methods. Results: İn 150 patients with diabetes; B. hominis was detected in 38 (25.3%) by PCR, in 34 (22.7%) by native-Lugol and trichrome staining. In the control group, 14 (14%) out of 100 subjects were positive by PCR, and 10 (10%) were positive by native-Lugol and trichrome staining. In the statistical evaluation, a significant difference was found between gender (P=0.023), age (P=0.045; ≤35 and >35 comparison), duration of diabetes (P=0.04), the HbA1c value (P=0.023; <8 and ≥8 comparison), and B. hominis positivity. ST1 was determined in 76.9% of patients with diabetes, and ST2 was determined in 23.07%. Considering the 3 methods, B. hominis positivity was detected in ...

Research paper thumbnail of Distribution of Toxocara vitulorum in Cattle of Ağrı Region

Turkish Journal of Parasitology

Amaç: Sığır, manda ve bizonların ince bağırsaklarında parazitlenen Toxocara vitulorum, Türkiye da... more Amaç: Sığır, manda ve bizonların ince bağırsaklarında parazitlenen Toxocara vitulorum, Türkiye dahil, nemli iklime sahip tropikal ve subtropikal iklim kuşağında bulunan ülkelerde görülmektedir. Erişkin parazitler özellikle buzağılarda iştahsızlık, zayıflama, ishal veya kabızlık gibi sindirim bozukluklarına ve bazen ölümlere neden olabilmektedir. Bu çalışma, Ağrı yöresi sığırlarında T. vitulorum'un yayılışını araştırmak amacıyla yapıldı. Yöntemler: Bu çalışmada, Ağrı ili ve ilçelerinde 0-6 aylık 200 buzağı ve 1 yaştan büyük 200 sığır rektumundan bir ceviz büyüklüğünde dışkı örneği alınarak, doymuş çinko sülfat flotasyon yöntemi ile preparatlar hazırlandı ve preparatlar ışık mikroskobunda incelendi. Bulgular: Dışkı örnekleri incelenen 0-6 aylık 200 buzağının 70'inde (%35), bir yaş üstü 200 sığırın 21'inde (%10,5) T. vitulorum yumurtası saptandı. Bir yaş üstü sığırlar ile buzağılar arasındaki pozitiflik farkı istatistiksel olarak anlamlı bulundu (p=0,001). Dışkı bakısı yapılan buzağıların 22'sinde (%11) ise Trichostrongylidae spp. yumurtaları saptandı.

Research paper thumbnail of Investigation of Giardia spp., Cryptosporidium spp. and Cyclospora cayetanensis in Samples Collected from Different Spring Waters Iğdır, Türkiye

Turkish Journal of Parasitology

In this study, it was aimed to investigate the physical and chemical properties of different spri... more In this study, it was aimed to investigate the physical and chemical properties of different spring waters and parasitic factors with different methods. Methods: This study was carried out on 69 water samples collected from different spring waters in and around Iğdır Province in April and June 2021. The samples were analyzed by native-Lugol, modified acid-fast staining, and nested polymerase chain reaction (nPCR). In addition, altitude (meter) and pressure (mmHg) measurements were made at the point where the water samples were taken. Results: One or more parasites were detected in 27.5% of the 69 water samples examined. Only C. cayetanensis was found in 13% of the samples, only Cryptosporidium spp. in 10.1%, only Giardia spp. in 1.4%, only C. cayetanensis and Giardia spp. in 1.4%, only C. cayetanensis, Cryptosporidium spp., and Giardia spp. in 1.4%. Only Giardia spp. cyst (4.3%) was detected by the direct examination method. While C. cayetanensis and Cryptosporidium spp. oocysts were detected in 8.7% and 7.2% of the samples by the modified acid-fast staining method, C. cayetanensis was detected in 15.9% and Cryptosporidium spp. was detected in 11.6% of the samples by nPCR. When the C. cayetanensis and Cryptosporidium spp. positivity rates were compared according to the characteristics of the water, there was no statistical difference between the altitude, salinity, pH, mmHg, and temperature (kelvin) values, but a significant correlation was found between the amount of dissolved oxygen and Cryptosporidium spp. positivity (p=0.047). Conclusion: Cryptosporidium spp., C. cayetanensis, and G. intestinalis are important waterborne pathogens that can cause epidemics. It is our belief that in order to reduce the risk of contamination of these parasitic factors with spring waters, public awareness should be raised, infrastructures should be improved, and new water treatment techniques, such as ultraviolet, ozonation and monitoring systems, should be used.

Research paper thumbnail of Global Trends on Blastocystis sp. Research: A Scientometric Study

Parasitologists United Journal

Research paper thumbnail of Sağlık Hizmetleri Meslek Yüksekokulu Öğrencilerinin Paraziter Hastalıklar Hakkındaki Bilgi Düzeyleri

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 2021

In this study, it has been aimed to determine the knowledge level of the students of Van Yuzuncu ... more In this study, it has been aimed to determine the knowledge level of the students of Van Yuzuncu Yıl University Health Services Vocational School, who will serve as the health personnel of the future, about parasitic diseases, prevention and control measures from these diseases. A total of 587 students, comprising 418 (71.2%) females and 169 (28.8%) males, studying at Van Yüzüncü Yıl University Health Services Vocational School, were included in the study. The questions in the questionnaire were arranged in three groups, as those about parasitic diseases, transmission routes of these diseases, and prevention and treatment. Cronbach alpha coefficients were calculated within the scope of the reliability analysis of the questionnaire questions. The Cronbach alpha values for the domains of Knowledge Level About Parasitic Diseases, Knowledge Level About the Transmission of Parasitic Diseases, and Knowledge Level About the Prevention and Treatment of Parasitic Diseases were determined as ...

Research paper thumbnail of Investigation of the Effect of Pasteurization on the Viability of Cryptosporidium parvum in Cow’s Milk by Propidium Monoazide qPCR

Research paper thumbnail of Investigation of the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis in immunosuppressed patients with diarrhea by IFA and real time PCR methods

Indian Journal of Medical Microbiology

Research paper thumbnail of Molecular Prevalence of Larval Stages of Fasciola hepatica in Lymnaea stagnalis Species Snails in the Vicinity of the Ağrı Province

Turkish Journal of Parasitology

Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fascio... more Büyük gölet salyangozu olarak bilinen Lymnaea stagnalis, zoonoz özellikte bir parazit olan Fasciola hepatica'nın ara konaklarından birisidir. Bu çalışmada Ağrı ili civarında toplanan L. stagnalis türü salyangozlarda F. hepatica'nın larval formlarının polimeraz zincir reaksiyonu (PZR) ile belirlenmesi amaçlanmıştır. Yöntemler: Bu çalışmada Ağrı ilinden toplam 150 adet L. stagnalis türü salyangoz toplanmıştır. Laboratuvara getirilen tatlı su salyangozları diseke edildikten sonra yumuşak dokuları mikroskop altında incelenmiştir. Diseke edilen salyangozlara DNA ekstraksiyonu işlemi yapılmıştır. DNA ekstraksiyonu sonrasında sitokrom c oksidaz alt birimi 1 gen bölgesini hedefleyen primerler kullanılarak PZR işlemi gerçekleştirilmiştir. Bulgular: Mikroskobik bakıda F. hepatica'nın larval formları tespit edilememiştir. Ancak PZR işlemi sonrasında yapılan analizde iki (%1,3) L. stagnalis tatlı su salyangozunun F. hepatica'nın larval formlarıyla enfekte olduğu sonucuna ulaşılmıştır.

Research paper thumbnail of Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method

Turkish Journal of Parasitology

Pneumocystis jirovecii'nin Nested PZR Yöntemi ile Araştırılması Objective: Pneumocystis jirovecii... more Pneumocystis jirovecii'nin Nested PZR Yöntemi ile Araştırılması Objective: Pneumocystis jirovecii (P. jirovecii) is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with P. jirovecii pneumonia (PJP) remains a key priority. This study investigated P. jirovecii in patients with lung cancer using the nested-polymerase chain reaction (PCR) method and examined the relationship between P. jirovecii and clinical findings. Methods: The study included 60 patients with lung cancer and 30 patients without lung cancer. The bronchoalveolar lavage (BAL) fluid samples of these 90 individuals were taken for diagnostic purposes in the University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Chest Diseases. Patient information was recorded. After DNA isolation from the BAL fluid samples taken from patients, the nested-PCR protocol for amplification of mtLSUrRNA in P. jirovecii was performed. Results: P. jirovecii DNA was detected in 40 (66.67%) of the lung cancer patients included in the study and in six (20%) patients without lung cancer, that is, in 46 (51.11%) patients. The rate of nested-PCR positivity in the lung cancer group was significantly higher than that in the non-lung cancer group (p=0.0001). Additionally, a statistically significant correlation was found between anorexia and weight loss, fever and sputum P. jirovecii positivity in patients with lung cancer (p<0.005). Conclusion: These findings suggest that lung cancer patients should be evaluated for PJP.

Research paper thumbnail of Global Trends on Blastocystis sp. Research: A Scientometric Study

Blastocystis sp. is the most common unicellular, luminal parasite in humans and animals. It has b... more Blastocystis sp. is the most common unicellular, luminal parasite in humans and animals. It has been associated with diarrhea and irritable bowel syndrome. This article describes the bibliometric research of articles published in Web of Science (WoS) database on Blastocystis sp. over a 51-year period. The VOSviewer visualization methodology was used for this study. Institutions, nations, international collaborations, journals, articles, authors, keywords, co-authors, co-citations, and citation rates from the WoS database formed the data of this study. A total of 1066 documents were found in the WoS database. Of which 773 were articles and only those publications were analyzed. The articles were cited 22,034 times in total and the mean Hirsch (H) index was 74. The countries with the highest number of articles were as follows: USA (n = 86; 11.125%), Singapore (n = 71; 9.185%), and Malaysia (n = 70; 9.056). Since 2000, there have been a limited number of articles and since 2014 there h...

Research paper thumbnail of Subtyping of Cryptosporidium parvum Obtained from Humans and Calves in Van, Turkey

Iranian Journal of Parasitology

Background: We aimed to investigate the prevalence of Cryptosporidium species detected in humans ... more Background: We aimed to investigate the prevalence of Cryptosporidium species detected in humans and calves in the Van region of Turkey. Methods: A total of 150 patients, comprising 60 who were immunosuppressed, 50 who were immunosuppressed and had diarrhea, and 40 who had only diarrhea, were enrolled in this study in the Department of Medical Parasitology, Van Yuzuncu Yıl University Faculty of Medicine, Turkey. Stool samples were taken from the rectums of a total of 50 calves that had 30 diarrhea and 20 that did not have diarrhea, from the stables and farms of 10 central villages of Van, Turkey. All samples were analyzed using modified acid-fast staining, immunochromatographic test, and PCR. Cryptosporidium positive samples were also subtyped. Results: Only C. parvum subtypes were detected in all positive samples. C. parvum was detected in 30 (20%) of the 150 human stool samples, while it was detected in 5 (10%) of the 50 samples from the calves. The GP60 gene region was amplified ...