Jarek Baran - Academia.edu (original) (raw)
Papers by Jarek Baran
DOAJ (DOAJ: Directory of Open Access Journals), Oct 1, 2011
This study was undertaken to determine how human pancreatic cancer (HPC-4) cells transduced with ... more This study was undertaken to determine how human pancreatic cancer (HPC-4) cells transduced with the TNF-GFP fusion gene (TLG) alter the antitumor response of human monocytes in vitro and whether they could act as an antitumor vaccine. In our model, HPC-4 cells were transduced with retroviral vector harboring TLG gene and designated as HPC-4(TLG). The TLG protein expression was confirmed by Western blot and flow cytometry analysis. Monocytes were co-cultured with transduced and control HPC-4 cells. The secretion of TNF, IL-10 and IL-12 was measured by ELISA. The cytotoxicity of monocytes against HPC-4 cells was determined by MTT test. The results show that the HPC-4(TLG) cells expressed membrane-bound, intracellular and secretory TLG protein. When cultured with HPC-4(TLG) cells, monocytes released a higher amount of TNF, but IL-10 and IL-12 secretion was inhibited. The pre-exposure of monocytes to HPC-4(TLG), but not to HPC-4, cells did not decrease TNF nor increase IL-10 production, thus not leading to monocyte deactivation. Also, the antitumor cytotoxicity of monocytes stimulated with HPC-4(TLG) was not downregulated, which occurred when non-transduced HPC-4 cells were used. In conclusion, compared to parental HPC-4 cells, TLG gene transduced HPC-4 cells induced stronger antitumor response of monocytes in vitro and prevented deactivation of monocytes.
PubMed, 2006
Monitoring of residual leukemic cells, so called minimal residual disease (MRD) in acute leukemia... more Monitoring of residual leukemic cells, so called minimal residual disease (MRD) in acute leukemias is of great importance in clinical practice. Especially evaluation of early response to treatment has a important prognostic value, and modifies the therapy schedule in a nowadays used treatment protocols. Methods used for monitoring of MRD consist of molecular techniques, which detect chromosomal aberrations or immunoglobulins and lymphocyte T receptor genes rearrangements, as well as method of flow cytometry, which detect immunophenotype typical for the leucemic clone. The paper shows advantages and disadvantages each of those methods. The best approach to monitoring MRD in ALL during and after the treatment seems to be combination of molecular and immunological techniques.
Applied Organometallic Chemistry, Jan 7, 2020
The effectiveness of PTT depends on the photosensitizer being a molecule which is toxic for the c... more The effectiveness of PTT depends on the photosensitizer being a molecule which is toxic for the cancer cells after electromagnetic wave irradiation. Therefore, a simulation of PTT was performed in this work on two colon cancer cells (SW480 and SW620) using platinum nanoparticles (Pt NPs). Interestingly, in the literature the dependence between the synthesis method and the photothermal properties of Pt NPs was not discussed. Consequently, in this paper, we evaluated the photothermal properties of Pt NPs synthesized by two different methods: polyol (PtI NPs) and green chemistry (PtII NPs). Scanning transmission electron microscopy revealed that the size of both Pt NPs obtained was 2 nm, the NPs were not agglomerated, and that the PtII NPs were distributed on green tea supports. The selected area electron diffraction and X-ray diffraction analysis confirmed the crystallinity of both types of Pt NPs. Fourier-transform infrared (FTIR) spectrum of the PtII NPs showed interactions between the NPs and stretching modes for C=O groups from flavonoids and polyphenols. Therefore, these chemical compounds could be responsible for reducing Pt 4+ ions to Pt 0. Moreover, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed that the PtII NPs exhibited 10% and 20% better cytotoxicity effect on SW480 and SW620 cells, than PtI NPs. The viability of cancer cells decreased when Pt NPs were used in PTT. The highest percentage of dead cells (82%) was observed for PtII NPs and 650-nm laser irradiation. FTIR and Raman spectroscopy showed structural changes induced by both Pt NPs and laser irradiation of cells in the range corresponding to levels of DNA, phospholipids, proteins, and lipids. Moreover, the calculated photothermal conversion efficiency showed that the value of this parameter is around 35%, regardless of the synthesis method and used wavelengths.
PubMed, Apr 1, 2011
Background: Tumour-derived microvesicles (TMVs) may interact with cells of the immune system. Our... more Background: Tumour-derived microvesicles (TMVs) may interact with cells of the immune system. Our previous observations indicated that TMVs modulate production of cytokines and reactive oxygen species (ROS) by monocytes. This study was designed to determine the role of TMVs in stimulation of chemokine production by human monocytes. Materials and methods: Chemokines at the mRNA and protein level were detected by real-time PCR and by Western blot, respecively. Chemokine release and chemotaxis of blood leukocytes were analysed by flow cytometry. Matrigel assay was used to determine angiogenesis in a NOD-SCID mice model. Results: TMVs induced secretion of interleukin-8 (CXCL8), monocyte chemoattractant protein-1 (CCL2), macrophage inflammatory protein-1α (CCL3) and MIP-1β (CCL4), and regulated on activation normal T-cells expressed and secreted (CCL5) chemokines and accumulation of their mRNA in monocytes. Moreover, TMVs enhanced angiogenesis in NOD-SCID mice by delivering chemokines and via stimulation of monocytes. In addition, TMVs may be storage for chemokines thus inducing chemotaxis of blood leukocytes. Conclusion: These results further support the role of TMVs in modulation of monocyte biological activity.
Research in Immunology, Sep 1, 1998
Journal of Materials Science, Jan 8, 2020
An easy synthesis method of hollow, porous gold nanoparticles (AuHP NPs) with controlled diameter... more An easy synthesis method of hollow, porous gold nanoparticles (AuHP NPs) with controlled diameter and pores sizes and with a wide range of light absorbance (continuous between 500 and 900 nm) is presented together with the explanation of the nanoparticle formation mechanism. The NPs were investigated using transmission electron microscopy (TEM) combined with the selected area electron diffraction patterns, X-ray diffraction and ultraviolet-visible spectroscopy. TEM images showed that changing the synthesis temperature allows to obtain AuHP NPs with sizes from 35 ± 4 nm at 60°C to 76 ± 8 nm at 90°C. The effects of nanoscale porosity on the far-and near-field optical properties of the nanoparticles, as well as on effective conversion of electromagnetic energy into thermal energy, were applied in simulated photothermal cancer therapy. The latter one was simulated by irradiation of two cancer cell lines SW480 and SW620 with lasers operating at 650 nm and 808 nm wavelengths. The mortality of cells after using the synthesized AuHP NPs as photosensitizers is between 20 and 50% and increases with the decrease in the diameter of the AuHP NPs. All these attractive properties of the AuHP NPs make them find application in many biomedical studies.
Medycyna Doświadczalna i Mikrobiologia, 1998
Journal of Materials Science, Nov 12, 2019
Photothermal therapy is a minimally invasive anticancer therapy, where the energy of light irradi... more Photothermal therapy is a minimally invasive anticancer therapy, where the energy of light irradiation is converted by photothermal agents to heat energy, thus increasing the temperature in the cancer cells. The efficiency of this therapy depends on the used photosensitizer, which must have several design criteria, such as plasmon resonance tenability and conversion efficiency. Based on these criteria, gold nanodahlias (AuD NPs) were synthesized and their anticancer properties were determined under irradiation with lasers operating at three different electromagnetic wavelengths (405 nm, 650 nm and 808 nm) of two colon cancer cell lines SW480 and SW620. Scanning and transmission electron microscopies revealed that the size of the synthesized AuD NPs is around 70 nm, while their UV-Vis spectrum showed a maximum absorbance value at 625 nm wavelength. The MTS assay showed that for 625 nm laser irradiation in the presence of NPs, the mortality of the two lines of cancer cells is around 70%, in comparison with control samples (untreated cancer cells). Fourier-transform infrared and Raman spectroscopy showed that the most visible chemical changes, especially in DNA, RNA, phospholipids, lipids and proteins functional groups, occur in the colon cancer cells cultured with AuD NPs irradiated with 650 nm and 808 nm lasers. A photothermal conversion efficiency reaching 50% is observed for AuD NPs irradiated with 650 nm and 808 nm wavelengths. All of these properties of AuD NPs suggest that these nanoparticles could be effective potential nanophotosensitizers in photothermal anticancer therapy.
Journal of Clinical Immunology, Mar 9, 2011
Chronic granulomatous disease (CGD) is phagocytic cell metabolic disorder resulting in recurrent ... more Chronic granulomatous disease (CGD) is phagocytic cell metabolic disorder resulting in recurrent infections and granuloma formation. This paper reports the favourable outcome of allogeneic transplantation in six high-risk CGD patients. The following donors were used: HLA-matched, related (two) and unrelated (three), and HLA-mismatched, unrelated (one). One patient was transplanted twice using the same sibling donor because of graft rejection at 6 months after reduced-intensity conditioning transplant (fludarabine and melphalan). Myeloablative conditioning regimen consisted of busulphan and cyclophosphamide. Stem cell source was unmanipulated bone marrow containing: 5.2 (2.6-6.5)×10 8 nucleated cells, 3.8 (2.0-8.0)×10 6 CD34+ cells and 45 (27-64)×10 6 CD3+ cells per kilogramme. Graft-versus-host disease prophylaxis consisted of cyclosporine A and, for unrelated donors, short course of methotrexate and anti-T-lymphocyte globulin. Mean neutrophile and platelet engraftments were observed at day 22 (20-23) and day 20 (16-29), respectively. Pre-existing infections and inflammatory granulomas resolved. With the follow-up of 4-35 months (mean, 20 months), all patients are alive and well with full donor chimerism and normalized superoxide production.
Pediatric Pulmonology, Mar 1, 1999
During bacterial infections of the respiratory tract, neutrophils (PMN) are recruited to the lung... more During bacterial infections of the respiratory tract, neutrophils (PMN) are recruited to the lung by various mechanisms, including production of interleukin-8 (IL-8) by alveolar macrophages (AM). After fulfilling their defense function, PMN become apoptotic and have to be disposed of by AM to prevent local damage to the lung tissue by oxygen species and proteolytic enzymes. We measured the levels of IL-8 in the bronchoalveolar lavage (BAL) and the ability of AM to engulf senescent PMN in a groups of children with and without recurrent infections of the respiratory tract. The IL-8 level was measured by enzyme-linked immunosorbent assay (ELISA). The phagocytosis of apoptotic neutrophils was evaluated microscopically by the presence of myeloperoxidase positive material in AM before and after 1 h of incubation with senescent PMN. The data show that children suffering from recurrent infections have increased IL-8 in BAL and that their AM have a lower ability to engulf apoptotic PMN in vitro. Furthermore, the proportion of annexin V-binding cells was higher in BAL of children with recurrent infections of the respiratory tract than in normal controls.
PubMed, 1999
The aim of this study was to investigate the interactions occurring between peripheral blood phag... more The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal elutriation were incubated with suspensions of opsonized bacteria. In parallel, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocyanate (FITC) and the percentage of cells containing FITC-labelled bacteria was analysed by flow cytometry. The data obtained show that strains of S. aureus originated from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes--strains isolated from the blood show the highest, but strains isolated from respiratory tract show the lowest sensitivity for killing. These strains differ too in their ability to trigger monocyte CL response. Contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
PubMed, 1998
The aim of this study was to investigate the interactions occurring between peripheral blood phag... more The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria. In parallel, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate the efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocynate (FITC) and the percentage of cells containing FITC-labelled bacteria were analysed by flow cytometry. The data obtained show the strains of S. aureus derived from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes--strains isolated from the blood show the highest, but strains isolated from the respiratory tract have the lowest sensitivity to killing. These strains differ too in their ability to trigger monocyte CL response. On the contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed a significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
Photodiagnosis and Photodynamic Therapy, Mar 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Pharmaceutics, Oct 18, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Journal of Molecular Sciences, Apr 6, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Research Square (Research Square), Aug 2, 2020
BMC Musculoskeletal Disorders, Dec 1, 2018
Background: Axial spondyloarthritis (axSpA) is characterized by significant bone loss caused by d... more Background: Axial spondyloarthritis (axSpA) is characterized by significant bone loss caused by dysregulation of physiological bone turnover, possibly resulting from intensified differentiation of osteoclasts. The aim of this study was to reevaluate the levels of osteoclastogenesis-mediating factors: soluble RANKL, M-CSF, OPG and other cytokines in sera of untreated, with sDMARDs and/or bDMARDs, axSpA patients and to test whether these sera influence differentiation of healthy monocytes towards osteoclast lineage. Methods: Bone remodeling molecules (RANKL, M-CSF, OPG, IL-6, OSM, IL-17A, TGFβ, and TNFα) were evaluated in 27 patients with axSpA and 23 age and sex-matched controls. Disease activity (BASDAI, ASDAS) and inflammatory markers (ESR, CRP) were assessed. Monocytes obtained from healthy individuals were cultured in vitro in presence of sera from 11 randomly chosen axSpA patients and 10 controls, with addition of exogenous M-CSF and/or RANKL or without. Osteoclastic differentiation was assessed analyzing osteoclast markers (cathepsin K and RANK at mRNA level) and with osteoclast-specific staining. Results: axSpA patients' sera levels of soluble RANKL were significantly lower and M-CSF, IL-6, OSM, IL-17A and TNFα significantly higher in comparison to controls, whereas of OPG and TGFβ were comparable in both groups. Numbers of generated in vitro osteoclasts and cathepsin K mRNA levels did not differ between cultures supplemented with sera of healthy and axSpA patients, both in the absence and presence of M-CSF. Instead, addition of exogenous RANKL boosted osteoclastogenesis, which was significantly higher in cultures with axSpA sera. Furthermore, sera from axSpA patients induced substantially higher levels of RANK mRNA, independently of M-CSF and RANKL stimulation. Conclusion: We show that, paradoxically, serum levels of soluble RANKL observed in axSpA are in fact significantly lower in comparison to healthy blood donors. Our results indicate that sera of axSpA patients-in contrary to healthy subjects-contain circulating, soluble factors (presumably IL-6, OSM, IL-17A, TNFα and others) able to stimulate healthy monocytes responsiveness to even relative low RANKL serum levels, by inducing high RANK mRNA expression and-as a net effect-boosting their osteoclastogenic potential. We suggest also that locally produced RANKL in axSpA may induce overactive osteoclasts from their precursors.
The threat of nuclear radiation terrorism has prompted a widespread search for defenses against t... more The threat of nuclear radiation terrorism has prompted a widespread search for defenses against this menace. Radiation destroys the bone marrow (BM) and depletes the body of white blood cells (WBC or leukocytes) that defend against infection and disease. In this immune-weakened state, the body is more susceptible to infection and may become overwhelmed before the immune system has time to recover. I.V. treatment with β-glucan, β(1,3) linked polymers of D-glucose isolated from the cell wall of baker's yeast, has previously been shown in mice to accelerate BM recovery and increase survival after lethal irradiation. Oral yeast β-glucan administration offers many practical and financial benefits as an alternative treatment, but has not been previously examined for a similar benefit in post-radiation myeloid recovery and survival. To test this hypothesis, mice were exposed to a sublethal dose of radiation (500 cGy) and treated daily with 80 µg of whole glucan particles (WGP Beta Glucan) by gastric gavage. This treatment with WGP Beta Glucan gave rise a to significantly faster recovery of leukocyte counts, as early as 7 days post-irradiation compared to control mice treated with PBS. A role for CR3 in mediating this hematopoietic recovery was established as oral WGP treatment in sublethally irradiated CR3 −/− (i.e. CD11b −/−) mice failed to enhance myeloid recovery. Complement receptor 3 (CR3, Mac-1, or CD11b/CD18) is a β 2-integrin found on monocytes, macrophages, and neutrophils that functions as a major leukocyte receptor for β-glucan, as well as for iC3b and ICAM-1. Subsequent investigation of mice that had been fed fluorescein-labeled WGP Beta Glucan revealed intestinal macrophages that had ingested WGP Beta Glucan and had migrated to the spleen and BM. Further experiments showed that wild-type, but not CR3 −/− macrophages, stimulated by WGP Beta Glucan synthesized IL-12 and several other inflammatory cytokines including GM-CSF. IL-12 is known to stimulate T lymphocyte production of oncostatin-M, a potent hematopoietic cytokine. In addition, the damaged BM removed from irradiated mice showed evidence for deposition of the serum complement protein C3 (iC3b-fragment) that was shown in vitro to serve as a co-stimulator of hematopoietic stem cell CR3 when added in combination with fragments of the β-glucan that are probably released by macrophages that convey WGP Beta Glucan to the BM. A role for serum complement C3 was further suggested because both C3-deficient (C3 −/−) and CR3 −/− mice exhibited a similar defect in recovery of blood leukocytes following sublethal radiation injury. While the exact mechanism of WGP Beta Glucan-enhanced myeloid recovery remains to be established, these data indicate that orally administered WGP Beta Glucan stimulates myeloid recovery following irradiation in a CR3-dependent manner. Consequently, oral treatment with WGP Beta Glucan may be a useful therapeutic intervention following radiation exposure to accelerate myeloid recovery after radiation exposure.
InTech eBooks, Jun 13, 2012
Many cell types including leukocytes, platelets and endothelial cells release small membrane frag... more Many cell types including leukocytes, platelets and endothelial cells release small membrane fragments called microparticles (MP). MP are shed during cell growth, activation, proliferation, senescence and apoptosis. MP contain proteins (intracellular as well as surface markers), mRNA and miRNA of the cells they have originated from. Based on the release mechanism, size and phenotype, MP are frequently divided into two categories: exosomes and ectosomes called also microvesicles. There is no doubt that the biological significance of MP has been largely overlooked for many years, regarding them as merely cellular fragments or debris. Nowadays, MP are being recognized as an important regulator of cellular interactions under physiological and pathological conditions. MP are present in all body fluids and physiologically serve various functions like blood clotting, enhance cell adhesiveness, increase cell aggregation, etc. They mediate cell-to-cell communication by transferring cell surface receptors, mRNA, and miRNA from the cell of origin to target cells. The growing body of literature regarding the role of MP in many pathologies has recently progressed from describing the association of elevated MP number with disease stage (e.g. cancer, sepsis) through understanding how MP may contribute to thrombosis, preeclampsia and tumor progression, and finally, to using MP as a source of antigens in new forms of vaccines against infectious or malignant diseases. Flow cytometry is a preferred method in the studies of MP because of its ability to quantitate the absolute number of particles and multicolor analysis attributes, allowing detection of several markers simultaneously. However, despite its usefulness, flow cytometry has some limitations in this field. The definition of MP using flow cytometry is still an area of great debate. In this review we propose a comprehensive summary of the possibilities, advantages and disadvantages of flow cytometry as a "gold standard" in the studies of MP. 2. Overview of different forms of microparticles 2.1 Definition of various MP-plenty is a plaque MP are defined as a mixture of heterogeneous vesicles size-wise, and there is a number of schemes trying to classify them by considering their different characteristics (i.e. origin, size, distinct cell surface and/or internal determinant patterns, etc.), which may become confusing at times.
Cells, Aug 19, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
DOAJ (DOAJ: Directory of Open Access Journals), Oct 1, 2011
This study was undertaken to determine how human pancreatic cancer (HPC-4) cells transduced with ... more This study was undertaken to determine how human pancreatic cancer (HPC-4) cells transduced with the TNF-GFP fusion gene (TLG) alter the antitumor response of human monocytes in vitro and whether they could act as an antitumor vaccine. In our model, HPC-4 cells were transduced with retroviral vector harboring TLG gene and designated as HPC-4(TLG). The TLG protein expression was confirmed by Western blot and flow cytometry analysis. Monocytes were co-cultured with transduced and control HPC-4 cells. The secretion of TNF, IL-10 and IL-12 was measured by ELISA. The cytotoxicity of monocytes against HPC-4 cells was determined by MTT test. The results show that the HPC-4(TLG) cells expressed membrane-bound, intracellular and secretory TLG protein. When cultured with HPC-4(TLG) cells, monocytes released a higher amount of TNF, but IL-10 and IL-12 secretion was inhibited. The pre-exposure of monocytes to HPC-4(TLG), but not to HPC-4, cells did not decrease TNF nor increase IL-10 production, thus not leading to monocyte deactivation. Also, the antitumor cytotoxicity of monocytes stimulated with HPC-4(TLG) was not downregulated, which occurred when non-transduced HPC-4 cells were used. In conclusion, compared to parental HPC-4 cells, TLG gene transduced HPC-4 cells induced stronger antitumor response of monocytes in vitro and prevented deactivation of monocytes.
PubMed, 2006
Monitoring of residual leukemic cells, so called minimal residual disease (MRD) in acute leukemia... more Monitoring of residual leukemic cells, so called minimal residual disease (MRD) in acute leukemias is of great importance in clinical practice. Especially evaluation of early response to treatment has a important prognostic value, and modifies the therapy schedule in a nowadays used treatment protocols. Methods used for monitoring of MRD consist of molecular techniques, which detect chromosomal aberrations or immunoglobulins and lymphocyte T receptor genes rearrangements, as well as method of flow cytometry, which detect immunophenotype typical for the leucemic clone. The paper shows advantages and disadvantages each of those methods. The best approach to monitoring MRD in ALL during and after the treatment seems to be combination of molecular and immunological techniques.
Applied Organometallic Chemistry, Jan 7, 2020
The effectiveness of PTT depends on the photosensitizer being a molecule which is toxic for the c... more The effectiveness of PTT depends on the photosensitizer being a molecule which is toxic for the cancer cells after electromagnetic wave irradiation. Therefore, a simulation of PTT was performed in this work on two colon cancer cells (SW480 and SW620) using platinum nanoparticles (Pt NPs). Interestingly, in the literature the dependence between the synthesis method and the photothermal properties of Pt NPs was not discussed. Consequently, in this paper, we evaluated the photothermal properties of Pt NPs synthesized by two different methods: polyol (PtI NPs) and green chemistry (PtII NPs). Scanning transmission electron microscopy revealed that the size of both Pt NPs obtained was 2 nm, the NPs were not agglomerated, and that the PtII NPs were distributed on green tea supports. The selected area electron diffraction and X-ray diffraction analysis confirmed the crystallinity of both types of Pt NPs. Fourier-transform infrared (FTIR) spectrum of the PtII NPs showed interactions between the NPs and stretching modes for C=O groups from flavonoids and polyphenols. Therefore, these chemical compounds could be responsible for reducing Pt 4+ ions to Pt 0. Moreover, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed that the PtII NPs exhibited 10% and 20% better cytotoxicity effect on SW480 and SW620 cells, than PtI NPs. The viability of cancer cells decreased when Pt NPs were used in PTT. The highest percentage of dead cells (82%) was observed for PtII NPs and 650-nm laser irradiation. FTIR and Raman spectroscopy showed structural changes induced by both Pt NPs and laser irradiation of cells in the range corresponding to levels of DNA, phospholipids, proteins, and lipids. Moreover, the calculated photothermal conversion efficiency showed that the value of this parameter is around 35%, regardless of the synthesis method and used wavelengths.
PubMed, Apr 1, 2011
Background: Tumour-derived microvesicles (TMVs) may interact with cells of the immune system. Our... more Background: Tumour-derived microvesicles (TMVs) may interact with cells of the immune system. Our previous observations indicated that TMVs modulate production of cytokines and reactive oxygen species (ROS) by monocytes. This study was designed to determine the role of TMVs in stimulation of chemokine production by human monocytes. Materials and methods: Chemokines at the mRNA and protein level were detected by real-time PCR and by Western blot, respecively. Chemokine release and chemotaxis of blood leukocytes were analysed by flow cytometry. Matrigel assay was used to determine angiogenesis in a NOD-SCID mice model. Results: TMVs induced secretion of interleukin-8 (CXCL8), monocyte chemoattractant protein-1 (CCL2), macrophage inflammatory protein-1α (CCL3) and MIP-1β (CCL4), and regulated on activation normal T-cells expressed and secreted (CCL5) chemokines and accumulation of their mRNA in monocytes. Moreover, TMVs enhanced angiogenesis in NOD-SCID mice by delivering chemokines and via stimulation of monocytes. In addition, TMVs may be storage for chemokines thus inducing chemotaxis of blood leukocytes. Conclusion: These results further support the role of TMVs in modulation of monocyte biological activity.
Research in Immunology, Sep 1, 1998
Journal of Materials Science, Jan 8, 2020
An easy synthesis method of hollow, porous gold nanoparticles (AuHP NPs) with controlled diameter... more An easy synthesis method of hollow, porous gold nanoparticles (AuHP NPs) with controlled diameter and pores sizes and with a wide range of light absorbance (continuous between 500 and 900 nm) is presented together with the explanation of the nanoparticle formation mechanism. The NPs were investigated using transmission electron microscopy (TEM) combined with the selected area electron diffraction patterns, X-ray diffraction and ultraviolet-visible spectroscopy. TEM images showed that changing the synthesis temperature allows to obtain AuHP NPs with sizes from 35 ± 4 nm at 60°C to 76 ± 8 nm at 90°C. The effects of nanoscale porosity on the far-and near-field optical properties of the nanoparticles, as well as on effective conversion of electromagnetic energy into thermal energy, were applied in simulated photothermal cancer therapy. The latter one was simulated by irradiation of two cancer cell lines SW480 and SW620 with lasers operating at 650 nm and 808 nm wavelengths. The mortality of cells after using the synthesized AuHP NPs as photosensitizers is between 20 and 50% and increases with the decrease in the diameter of the AuHP NPs. All these attractive properties of the AuHP NPs make them find application in many biomedical studies.
Medycyna Doświadczalna i Mikrobiologia, 1998
Journal of Materials Science, Nov 12, 2019
Photothermal therapy is a minimally invasive anticancer therapy, where the energy of light irradi... more Photothermal therapy is a minimally invasive anticancer therapy, where the energy of light irradiation is converted by photothermal agents to heat energy, thus increasing the temperature in the cancer cells. The efficiency of this therapy depends on the used photosensitizer, which must have several design criteria, such as plasmon resonance tenability and conversion efficiency. Based on these criteria, gold nanodahlias (AuD NPs) were synthesized and their anticancer properties were determined under irradiation with lasers operating at three different electromagnetic wavelengths (405 nm, 650 nm and 808 nm) of two colon cancer cell lines SW480 and SW620. Scanning and transmission electron microscopies revealed that the size of the synthesized AuD NPs is around 70 nm, while their UV-Vis spectrum showed a maximum absorbance value at 625 nm wavelength. The MTS assay showed that for 625 nm laser irradiation in the presence of NPs, the mortality of the two lines of cancer cells is around 70%, in comparison with control samples (untreated cancer cells). Fourier-transform infrared and Raman spectroscopy showed that the most visible chemical changes, especially in DNA, RNA, phospholipids, lipids and proteins functional groups, occur in the colon cancer cells cultured with AuD NPs irradiated with 650 nm and 808 nm lasers. A photothermal conversion efficiency reaching 50% is observed for AuD NPs irradiated with 650 nm and 808 nm wavelengths. All of these properties of AuD NPs suggest that these nanoparticles could be effective potential nanophotosensitizers in photothermal anticancer therapy.
Journal of Clinical Immunology, Mar 9, 2011
Chronic granulomatous disease (CGD) is phagocytic cell metabolic disorder resulting in recurrent ... more Chronic granulomatous disease (CGD) is phagocytic cell metabolic disorder resulting in recurrent infections and granuloma formation. This paper reports the favourable outcome of allogeneic transplantation in six high-risk CGD patients. The following donors were used: HLA-matched, related (two) and unrelated (three), and HLA-mismatched, unrelated (one). One patient was transplanted twice using the same sibling donor because of graft rejection at 6 months after reduced-intensity conditioning transplant (fludarabine and melphalan). Myeloablative conditioning regimen consisted of busulphan and cyclophosphamide. Stem cell source was unmanipulated bone marrow containing: 5.2 (2.6-6.5)×10 8 nucleated cells, 3.8 (2.0-8.0)×10 6 CD34+ cells and 45 (27-64)×10 6 CD3+ cells per kilogramme. Graft-versus-host disease prophylaxis consisted of cyclosporine A and, for unrelated donors, short course of methotrexate and anti-T-lymphocyte globulin. Mean neutrophile and platelet engraftments were observed at day 22 (20-23) and day 20 (16-29), respectively. Pre-existing infections and inflammatory granulomas resolved. With the follow-up of 4-35 months (mean, 20 months), all patients are alive and well with full donor chimerism and normalized superoxide production.
Pediatric Pulmonology, Mar 1, 1999
During bacterial infections of the respiratory tract, neutrophils (PMN) are recruited to the lung... more During bacterial infections of the respiratory tract, neutrophils (PMN) are recruited to the lung by various mechanisms, including production of interleukin-8 (IL-8) by alveolar macrophages (AM). After fulfilling their defense function, PMN become apoptotic and have to be disposed of by AM to prevent local damage to the lung tissue by oxygen species and proteolytic enzymes. We measured the levels of IL-8 in the bronchoalveolar lavage (BAL) and the ability of AM to engulf senescent PMN in a groups of children with and without recurrent infections of the respiratory tract. The IL-8 level was measured by enzyme-linked immunosorbent assay (ELISA). The phagocytosis of apoptotic neutrophils was evaluated microscopically by the presence of myeloperoxidase positive material in AM before and after 1 h of incubation with senescent PMN. The data show that children suffering from recurrent infections have increased IL-8 in BAL and that their AM have a lower ability to engulf apoptotic PMN in vitro. Furthermore, the proportion of annexin V-binding cells was higher in BAL of children with recurrent infections of the respiratory tract than in normal controls.
PubMed, 1999
The aim of this study was to investigate the interactions occurring between peripheral blood phag... more The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal elutriation were incubated with suspensions of opsonized bacteria. In parallel, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocyanate (FITC) and the percentage of cells containing FITC-labelled bacteria was analysed by flow cytometry. The data obtained show that strains of S. aureus originated from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes--strains isolated from the blood show the highest, but strains isolated from respiratory tract show the lowest sensitivity for killing. These strains differ too in their ability to trigger monocyte CL response. Contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
PubMed, 1998
The aim of this study was to investigate the interactions occurring between peripheral blood phag... more The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria. In parallel, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate the efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocynate (FITC) and the percentage of cells containing FITC-labelled bacteria were analysed by flow cytometry. The data obtained show the strains of S. aureus derived from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes--strains isolated from the blood show the highest, but strains isolated from the respiratory tract have the lowest sensitivity to killing. These strains differ too in their ability to trigger monocyte CL response. On the contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed a significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
Photodiagnosis and Photodynamic Therapy, Mar 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Pharmaceutics, Oct 18, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Journal of Molecular Sciences, Apr 6, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Research Square (Research Square), Aug 2, 2020
BMC Musculoskeletal Disorders, Dec 1, 2018
Background: Axial spondyloarthritis (axSpA) is characterized by significant bone loss caused by d... more Background: Axial spondyloarthritis (axSpA) is characterized by significant bone loss caused by dysregulation of physiological bone turnover, possibly resulting from intensified differentiation of osteoclasts. The aim of this study was to reevaluate the levels of osteoclastogenesis-mediating factors: soluble RANKL, M-CSF, OPG and other cytokines in sera of untreated, with sDMARDs and/or bDMARDs, axSpA patients and to test whether these sera influence differentiation of healthy monocytes towards osteoclast lineage. Methods: Bone remodeling molecules (RANKL, M-CSF, OPG, IL-6, OSM, IL-17A, TGFβ, and TNFα) were evaluated in 27 patients with axSpA and 23 age and sex-matched controls. Disease activity (BASDAI, ASDAS) and inflammatory markers (ESR, CRP) were assessed. Monocytes obtained from healthy individuals were cultured in vitro in presence of sera from 11 randomly chosen axSpA patients and 10 controls, with addition of exogenous M-CSF and/or RANKL or without. Osteoclastic differentiation was assessed analyzing osteoclast markers (cathepsin K and RANK at mRNA level) and with osteoclast-specific staining. Results: axSpA patients' sera levels of soluble RANKL were significantly lower and M-CSF, IL-6, OSM, IL-17A and TNFα significantly higher in comparison to controls, whereas of OPG and TGFβ were comparable in both groups. Numbers of generated in vitro osteoclasts and cathepsin K mRNA levels did not differ between cultures supplemented with sera of healthy and axSpA patients, both in the absence and presence of M-CSF. Instead, addition of exogenous RANKL boosted osteoclastogenesis, which was significantly higher in cultures with axSpA sera. Furthermore, sera from axSpA patients induced substantially higher levels of RANK mRNA, independently of M-CSF and RANKL stimulation. Conclusion: We show that, paradoxically, serum levels of soluble RANKL observed in axSpA are in fact significantly lower in comparison to healthy blood donors. Our results indicate that sera of axSpA patients-in contrary to healthy subjects-contain circulating, soluble factors (presumably IL-6, OSM, IL-17A, TNFα and others) able to stimulate healthy monocytes responsiveness to even relative low RANKL serum levels, by inducing high RANK mRNA expression and-as a net effect-boosting their osteoclastogenic potential. We suggest also that locally produced RANKL in axSpA may induce overactive osteoclasts from their precursors.
The threat of nuclear radiation terrorism has prompted a widespread search for defenses against t... more The threat of nuclear radiation terrorism has prompted a widespread search for defenses against this menace. Radiation destroys the bone marrow (BM) and depletes the body of white blood cells (WBC or leukocytes) that defend against infection and disease. In this immune-weakened state, the body is more susceptible to infection and may become overwhelmed before the immune system has time to recover. I.V. treatment with β-glucan, β(1,3) linked polymers of D-glucose isolated from the cell wall of baker's yeast, has previously been shown in mice to accelerate BM recovery and increase survival after lethal irradiation. Oral yeast β-glucan administration offers many practical and financial benefits as an alternative treatment, but has not been previously examined for a similar benefit in post-radiation myeloid recovery and survival. To test this hypothesis, mice were exposed to a sublethal dose of radiation (500 cGy) and treated daily with 80 µg of whole glucan particles (WGP Beta Glucan) by gastric gavage. This treatment with WGP Beta Glucan gave rise a to significantly faster recovery of leukocyte counts, as early as 7 days post-irradiation compared to control mice treated with PBS. A role for CR3 in mediating this hematopoietic recovery was established as oral WGP treatment in sublethally irradiated CR3 −/− (i.e. CD11b −/−) mice failed to enhance myeloid recovery. Complement receptor 3 (CR3, Mac-1, or CD11b/CD18) is a β 2-integrin found on monocytes, macrophages, and neutrophils that functions as a major leukocyte receptor for β-glucan, as well as for iC3b and ICAM-1. Subsequent investigation of mice that had been fed fluorescein-labeled WGP Beta Glucan revealed intestinal macrophages that had ingested WGP Beta Glucan and had migrated to the spleen and BM. Further experiments showed that wild-type, but not CR3 −/− macrophages, stimulated by WGP Beta Glucan synthesized IL-12 and several other inflammatory cytokines including GM-CSF. IL-12 is known to stimulate T lymphocyte production of oncostatin-M, a potent hematopoietic cytokine. In addition, the damaged BM removed from irradiated mice showed evidence for deposition of the serum complement protein C3 (iC3b-fragment) that was shown in vitro to serve as a co-stimulator of hematopoietic stem cell CR3 when added in combination with fragments of the β-glucan that are probably released by macrophages that convey WGP Beta Glucan to the BM. A role for serum complement C3 was further suggested because both C3-deficient (C3 −/−) and CR3 −/− mice exhibited a similar defect in recovery of blood leukocytes following sublethal radiation injury. While the exact mechanism of WGP Beta Glucan-enhanced myeloid recovery remains to be established, these data indicate that orally administered WGP Beta Glucan stimulates myeloid recovery following irradiation in a CR3-dependent manner. Consequently, oral treatment with WGP Beta Glucan may be a useful therapeutic intervention following radiation exposure to accelerate myeloid recovery after radiation exposure.
InTech eBooks, Jun 13, 2012
Many cell types including leukocytes, platelets and endothelial cells release small membrane frag... more Many cell types including leukocytes, platelets and endothelial cells release small membrane fragments called microparticles (MP). MP are shed during cell growth, activation, proliferation, senescence and apoptosis. MP contain proteins (intracellular as well as surface markers), mRNA and miRNA of the cells they have originated from. Based on the release mechanism, size and phenotype, MP are frequently divided into two categories: exosomes and ectosomes called also microvesicles. There is no doubt that the biological significance of MP has been largely overlooked for many years, regarding them as merely cellular fragments or debris. Nowadays, MP are being recognized as an important regulator of cellular interactions under physiological and pathological conditions. MP are present in all body fluids and physiologically serve various functions like blood clotting, enhance cell adhesiveness, increase cell aggregation, etc. They mediate cell-to-cell communication by transferring cell surface receptors, mRNA, and miRNA from the cell of origin to target cells. The growing body of literature regarding the role of MP in many pathologies has recently progressed from describing the association of elevated MP number with disease stage (e.g. cancer, sepsis) through understanding how MP may contribute to thrombosis, preeclampsia and tumor progression, and finally, to using MP as a source of antigens in new forms of vaccines against infectious or malignant diseases. Flow cytometry is a preferred method in the studies of MP because of its ability to quantitate the absolute number of particles and multicolor analysis attributes, allowing detection of several markers simultaneously. However, despite its usefulness, flow cytometry has some limitations in this field. The definition of MP using flow cytometry is still an area of great debate. In this review we propose a comprehensive summary of the possibilities, advantages and disadvantages of flow cytometry as a "gold standard" in the studies of MP. 2. Overview of different forms of microparticles 2.1 Definition of various MP-plenty is a plaque MP are defined as a mixture of heterogeneous vesicles size-wise, and there is a number of schemes trying to classify them by considering their different characteristics (i.e. origin, size, distinct cell surface and/or internal determinant patterns, etc.), which may become confusing at times.
Cells, Aug 19, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY