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Papers by Karolina Bazela

Research paper thumbnail of Communication L-Ergothioneine Protects Skin Cells against UV-Induced Damage—A Preliminary Study

Research paper thumbnail of Modulation of cellular prion protein (PrPc) expression in the gastrointestinal tract

Research paper thumbnail of Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possible link to prion disease

World Journal of Gastroenterology, 2005

Schuppan D. Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possibl... more Schuppan D. Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possible link to prion disease.

Research paper thumbnail of Modulation of cellular prion protein (PrPc) expression in the gastrointestinal tract

Archiwum Uj Dokt 2004 183, 2004

Research paper thumbnail of Designing of biodegradable and biocompatible release and delivery systems of selected antioxidants used in cosmetology

Biomacromolecules, Jan 7, 2015

Conjugates of antioxidants: p-anisic and vanillic acids with non-toxic, biocompatible and biodegr... more Conjugates of antioxidants: p-anisic and vanillic acids with non-toxic, biocompatible and biodegradedable oligo-(R,S)-(3-hydoxybutyrate) carrier were synthesized and their structural and biological characterization was performed. The molecular structure of the bioconjugates, in which antioxidants are covalently bonded with oligo(3-hydroxybutyrate) chains, has been proven by mass spectrometry supported by NMR. The bioconjugates hydrolytic degradation studies allowed to gain thorough insight into the hydrolysis process and confirmed the release of p-anisic acid and vanillic acid. In vitro studies demonstrated that all of the conjugates studied were well tolerated by KB and HaCaT cell lines as they had no marked cytotoxicity, while conjugates with a relatively short OHB carrier are optimal to support keratinocyte function. The preliminary study of the biological activity confirmed the protective effect of VA-OHB conjugates against H2O2-induced lipid peroxidation in human keratinocytes ...

Research paper thumbnail of l-Ergothioneine Protects Skin Cells against UV-Induced Damage—A Preliminary Study

Cosmetics, 2014

Many changes related to aging at the cellular level may be due to the physiological condition of ... more Many changes related to aging at the cellular level may be due to the physiological condition of mitochondria. One of the most common types of damage of mtDNA is the so-called "common deletion" referring to a deletion of 4977 base pairs. In the skin cells this phenomenon probably is caused by oxidative damage of mtDNA induced by UV. The present study was aimed at evaluating the effect of the antioxidant L-ergothioneine on UV-induced damage in skin cells. The effect of L-ergothioneine on the reduced glutathione level was studied. The presence of the "common deletion" in human fibroblasts irradiated with UVA and treated with L-ergothioneine was evaluated by a polymerase chain reaction. We have demonstrated that L-ergothioneine enhanced the level of reduced glutathione and protected cells from the induction of a photoaging-associated mtDNA "common deletion". In view of our results, L-ergothioneine could be an effective skin care and anti-photoaging ingredient.

Research paper thumbnail of Hepatocyte growth factor (HGF) and proinflammatory cytokines induce gastrin in hepatic cells: an important link in the development of hepatocellular carcinoma (HCC)??

Gastroenterology, 2003

Pancreatic samples exhibited strong cell membrane staining within the Islets of Langerhans. Liver... more Pancreatic samples exhibited strong cell membrane staining within the Islets of Langerhans. Liver samples exhibited strong staining in portal tract lymphocytes, Kupffer cells and endothelinm. Lymphocytes in all gastrointestinal tissues stained strongly. Tissues not incubated with anti-hENT1 mAb were not stained. Conclusion: We report a novel immunohistoebemical assay and the first known immunohistochemieal localization of hENT 1 in human gastrointestinal tissues. This localization supports functional studies that eqinlibrative NT proteins are predominately found on the basolateral aspect of epithehal cells. As the absence of hENT1 confers high level nucleoside analogue drug resistance, this assay may serve as an important predictive tool in nucleoside analogue based gastrointestinal cancer therapies. CKWW is the recipient of an AHFMR Clinical Fellowship. M979 Hepatocyte growth factor (HGF) and proinflammatory cytokines induce gastrin in hepatic cells: an important link in the development of hepatocellular carcinoma (HCC) ??

Research paper thumbnail of Central and peripheral gastroprotective action of melatonin and its precursor, L-tryptophan, against stress-induced gastric lesions

Gastroenterology, 2003

Expr~on o(adhesion molecules In human monocytes (% change v$ control) GMtdn 10"eM Gutrln 10"M Oas... more Expr~on o(adhesion molecules In human monocytes (% change v$ control) GMtdn 10"eM Gutrln 10"M Oastrln tO~TM L.Selectln-14 ~ 5-14 ~ 7-23 * 15 CD18 2 ~ 6 13 ~ 4*

Research paper thumbnail of Impaired gastric ulcer healing in diabetic rats: role of heat shock protein, growth factors, prostaglandins and proinflammatory cytokines

European Journal of Pharmacology, 2003

Gastric mucosa of diabetic rats is highly vulnerable to acute injury, but little is known about t... more Gastric mucosa of diabetic rats is highly vulnerable to acute injury, but little is known about the influence of diabetic conditions on the healing of gastric ulcers. In this study, streptozotocin (70 mg/kg injected intraperitoneally) was used to induce diabetes mellitus in rats. Four weeks after streptozotocin injection, gastric ulcers were induced using the acetic acid method, and 10 days later, the healing rate and the gastric blood flow (GBF) were measured by planimetry and hydrogen (H(2))-gas clearance method, respectively. Six major groups of rats with gastric ulcers were used: (1) vehicle (saline); (2) streptozotocin alone; (3) insulin (4 IU/day intraperitoneally); (4) streptozotocin plus insulin; (5) pentoxifylline, an inhibitor of synthesis and release of tumor necrosis factor-alpha (TNF alpha); and (6) aspirin, a non-selective inhibitor of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), and rofecoxib, the highly selective COX-2. In the diabetic rats, a significant delay in ulcer healing ( approximately by 300%), accompanied by a decrease in the gastric mucosal blood flow was observed. The prolongation of the healing in diabetic animals was associated with an increase in gastric mucosal expression and release of TNFalpha, interleukin-1 beta (IL-1 beta), suppression of the vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (PECAM-1) and the mucosal overexpression of heat shock protein 70 (HSP 70). Administration of insulin reversed the delay in ulcer healing and significantly decreased the expression of IL-1 beta and TNF-alpha, while producing the rise in the expression of VEGF and PECAM. Pentoxifylline, an inhibitor of TNF-alpha, which by itself accelerated ulcer healing in non-diabetic rats, counteracted the increase in the area of gastric ulcer induced by streptozotocin, raised significantly gastric blood flow and suppressed the plasma TNF-alpha levels. Aspirin and rofecoxib, that significantly suppressed the mucosal prostaglandin E(2) generation in ulcer area, delayed significantly the rate of ulcer healing and decreased the GBF at ulcer margin in non-diabetic rats, and these changes were significantly augmented in diabetic animals. We conclude that: (1) Experimental diabetes dramatically impairs ulcer healing, depending upon the increased release of proinflammatory cytokines and the attenuation of angiogenesis that can limit the ulcer healing effects of locally produced HSP 70 and TNF-alpha. (2) Insulin reversed this impairment of ulcer healing in diabetic rats, mainly due to the enhancement of angiogenesis and reduction in expression of cytokines in the ulcer area. (3) Classic non-steroidal anti-inflammatory drugs such as aspirin prolonged ulcer healing under diabetic conditions due to suppression of endogenous prostaglandins and the fall in the microcirculation at the ulcer margin and these effects were mimicked by selective, so called "safe" COX-2 inhibitor, rofecoxib, suggesting that both COX isoforms are important sources of prostaglandins that are essential in the ulcer healing in diabetes.

Research paper thumbnail of Pioglitazone, a specific ligand of peroxisome proliferator-activated receptor-gamma, accelerates gastric ulcer healing in rat

European Journal of Pharmacology, 2003

Research paper thumbnail of The repair effect of Folacin on skin damage due to radiotherapy

Kosmetische …, 2007

Zusammenfassung Obwohl medizinische und pharmakologische Fortschritte unübersehbar sind, ist die ... more Zusammenfassung Obwohl medizinische und pharmakologische Fortschritte unübersehbar sind, ist die Behandlung bösartiger Tumore nach wie vor mit einem hohen Risiko unerwünschter Nebenwirkungen verbunden. Insbesondere bei der Strahlentherapie sind ...

Research paper thumbnail of Protective effect of Folacin on UVB-induced skin damage

15-th Congress of the …, 2006

Discussion The main repair mechanism for UVB-induced DNA damage in mammalian cells is nucleotide ... more Discussion The main repair mechanism for UVB-induced DNA damage in mammalian cells is nucleotide excision repair (NER). Our preliminary results showed that Folacin treatment enhances comet length after UV exposure, which meant that 3 it might induce NER. It is ...

Research paper thumbnail of The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene

BMC Complementary and Alternative Medicine, 2012

Research paper thumbnail of Communication L-Ergothioneine Protects Skin Cells against UV-Induced Damage—A Preliminary Study

Research paper thumbnail of Modulation of cellular prion protein (PrPc) expression in the gastrointestinal tract

Research paper thumbnail of Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possible link to prion disease

World Journal of Gastroenterology, 2005

Schuppan D. Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possibl... more Schuppan D. Helicobacter pylori upregulates prion protein expression in gastric mucosa: A possible link to prion disease.

Research paper thumbnail of Modulation of cellular prion protein (PrPc) expression in the gastrointestinal tract

Archiwum Uj Dokt 2004 183, 2004

Research paper thumbnail of Designing of biodegradable and biocompatible release and delivery systems of selected antioxidants used in cosmetology

Biomacromolecules, Jan 7, 2015

Conjugates of antioxidants: p-anisic and vanillic acids with non-toxic, biocompatible and biodegr... more Conjugates of antioxidants: p-anisic and vanillic acids with non-toxic, biocompatible and biodegradedable oligo-(R,S)-(3-hydoxybutyrate) carrier were synthesized and their structural and biological characterization was performed. The molecular structure of the bioconjugates, in which antioxidants are covalently bonded with oligo(3-hydroxybutyrate) chains, has been proven by mass spectrometry supported by NMR. The bioconjugates hydrolytic degradation studies allowed to gain thorough insight into the hydrolysis process and confirmed the release of p-anisic acid and vanillic acid. In vitro studies demonstrated that all of the conjugates studied were well tolerated by KB and HaCaT cell lines as they had no marked cytotoxicity, while conjugates with a relatively short OHB carrier are optimal to support keratinocyte function. The preliminary study of the biological activity confirmed the protective effect of VA-OHB conjugates against H2O2-induced lipid peroxidation in human keratinocytes ...

Research paper thumbnail of l-Ergothioneine Protects Skin Cells against UV-Induced Damage—A Preliminary Study

Cosmetics, 2014

Many changes related to aging at the cellular level may be due to the physiological condition of ... more Many changes related to aging at the cellular level may be due to the physiological condition of mitochondria. One of the most common types of damage of mtDNA is the so-called "common deletion" referring to a deletion of 4977 base pairs. In the skin cells this phenomenon probably is caused by oxidative damage of mtDNA induced by UV. The present study was aimed at evaluating the effect of the antioxidant L-ergothioneine on UV-induced damage in skin cells. The effect of L-ergothioneine on the reduced glutathione level was studied. The presence of the "common deletion" in human fibroblasts irradiated with UVA and treated with L-ergothioneine was evaluated by a polymerase chain reaction. We have demonstrated that L-ergothioneine enhanced the level of reduced glutathione and protected cells from the induction of a photoaging-associated mtDNA "common deletion". In view of our results, L-ergothioneine could be an effective skin care and anti-photoaging ingredient.

Research paper thumbnail of Hepatocyte growth factor (HGF) and proinflammatory cytokines induce gastrin in hepatic cells: an important link in the development of hepatocellular carcinoma (HCC)??

Gastroenterology, 2003

Pancreatic samples exhibited strong cell membrane staining within the Islets of Langerhans. Liver... more Pancreatic samples exhibited strong cell membrane staining within the Islets of Langerhans. Liver samples exhibited strong staining in portal tract lymphocytes, Kupffer cells and endothelinm. Lymphocytes in all gastrointestinal tissues stained strongly. Tissues not incubated with anti-hENT1 mAb were not stained. Conclusion: We report a novel immunohistoebemical assay and the first known immunohistochemieal localization of hENT 1 in human gastrointestinal tissues. This localization supports functional studies that eqinlibrative NT proteins are predominately found on the basolateral aspect of epithehal cells. As the absence of hENT1 confers high level nucleoside analogue drug resistance, this assay may serve as an important predictive tool in nucleoside analogue based gastrointestinal cancer therapies. CKWW is the recipient of an AHFMR Clinical Fellowship. M979 Hepatocyte growth factor (HGF) and proinflammatory cytokines induce gastrin in hepatic cells: an important link in the development of hepatocellular carcinoma (HCC) ??

Research paper thumbnail of Central and peripheral gastroprotective action of melatonin and its precursor, L-tryptophan, against stress-induced gastric lesions

Gastroenterology, 2003

Expr~on o(adhesion molecules In human monocytes (% change v$ control) GMtdn 10"eM Gutrln 10"M Oas... more Expr~on o(adhesion molecules In human monocytes (% change v$ control) GMtdn 10"eM Gutrln 10"M Oastrln tO~TM L.Selectln-14 ~ 5-14 ~ 7-23 * 15 CD18 2 ~ 6 13 ~ 4*

Research paper thumbnail of Impaired gastric ulcer healing in diabetic rats: role of heat shock protein, growth factors, prostaglandins and proinflammatory cytokines

European Journal of Pharmacology, 2003

Gastric mucosa of diabetic rats is highly vulnerable to acute injury, but little is known about t... more Gastric mucosa of diabetic rats is highly vulnerable to acute injury, but little is known about the influence of diabetic conditions on the healing of gastric ulcers. In this study, streptozotocin (70 mg/kg injected intraperitoneally) was used to induce diabetes mellitus in rats. Four weeks after streptozotocin injection, gastric ulcers were induced using the acetic acid method, and 10 days later, the healing rate and the gastric blood flow (GBF) were measured by planimetry and hydrogen (H(2))-gas clearance method, respectively. Six major groups of rats with gastric ulcers were used: (1) vehicle (saline); (2) streptozotocin alone; (3) insulin (4 IU/day intraperitoneally); (4) streptozotocin plus insulin; (5) pentoxifylline, an inhibitor of synthesis and release of tumor necrosis factor-alpha (TNF alpha); and (6) aspirin, a non-selective inhibitor of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), and rofecoxib, the highly selective COX-2. In the diabetic rats, a significant delay in ulcer healing ( approximately by 300%), accompanied by a decrease in the gastric mucosal blood flow was observed. The prolongation of the healing in diabetic animals was associated with an increase in gastric mucosal expression and release of TNFalpha, interleukin-1 beta (IL-1 beta), suppression of the vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (PECAM-1) and the mucosal overexpression of heat shock protein 70 (HSP 70). Administration of insulin reversed the delay in ulcer healing and significantly decreased the expression of IL-1 beta and TNF-alpha, while producing the rise in the expression of VEGF and PECAM. Pentoxifylline, an inhibitor of TNF-alpha, which by itself accelerated ulcer healing in non-diabetic rats, counteracted the increase in the area of gastric ulcer induced by streptozotocin, raised significantly gastric blood flow and suppressed the plasma TNF-alpha levels. Aspirin and rofecoxib, that significantly suppressed the mucosal prostaglandin E(2) generation in ulcer area, delayed significantly the rate of ulcer healing and decreased the GBF at ulcer margin in non-diabetic rats, and these changes were significantly augmented in diabetic animals. We conclude that: (1) Experimental diabetes dramatically impairs ulcer healing, depending upon the increased release of proinflammatory cytokines and the attenuation of angiogenesis that can limit the ulcer healing effects of locally produced HSP 70 and TNF-alpha. (2) Insulin reversed this impairment of ulcer healing in diabetic rats, mainly due to the enhancement of angiogenesis and reduction in expression of cytokines in the ulcer area. (3) Classic non-steroidal anti-inflammatory drugs such as aspirin prolonged ulcer healing under diabetic conditions due to suppression of endogenous prostaglandins and the fall in the microcirculation at the ulcer margin and these effects were mimicked by selective, so called "safe" COX-2 inhibitor, rofecoxib, suggesting that both COX isoforms are important sources of prostaglandins that are essential in the ulcer healing in diabetes.

Research paper thumbnail of Pioglitazone, a specific ligand of peroxisome proliferator-activated receptor-gamma, accelerates gastric ulcer healing in rat

European Journal of Pharmacology, 2003

Research paper thumbnail of The repair effect of Folacin on skin damage due to radiotherapy

Kosmetische …, 2007

Zusammenfassung Obwohl medizinische und pharmakologische Fortschritte unübersehbar sind, ist die ... more Zusammenfassung Obwohl medizinische und pharmakologische Fortschritte unübersehbar sind, ist die Behandlung bösartiger Tumore nach wie vor mit einem hohen Risiko unerwünschter Nebenwirkungen verbunden. Insbesondere bei der Strahlentherapie sind ...

Research paper thumbnail of Protective effect of Folacin on UVB-induced skin damage

15-th Congress of the …, 2006

Discussion The main repair mechanism for UVB-induced DNA damage in mammalian cells is nucleotide ... more Discussion The main repair mechanism for UVB-induced DNA damage in mammalian cells is nucleotide excision repair (NER). Our preliminary results showed that Folacin treatment enhances comet length after UV exposure, which meant that 3 it might induce NER. It is ...

Research paper thumbnail of The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene

BMC Complementary and Alternative Medicine, 2012