Beata Krawczyk - Academia.edu (original) (raw)

Papers by Beata Krawczyk

Urinary tract infection caused by Escherichia coli can lead to urosepsis, and the pathogens’ effe... more Urinary tract infection caused by Escherichia coli can lead to urosepsis, and the pathogens’ effectiveness in acquiring iron determines their virulence. The level of expression of the siderophore genes in different environments (M9 medium, artificial urine, and M9 supplemented with blood) was investigated using the CAS assay and qPCR. Aerobactin was found much more frequently in blood than in urine isolates (P=0.03), but the expression in artificial urine was 2x higher than in blood (P=0.03). We observed significant changes in the expression of entC, iro-2, iucA, and iroB bacterial genes between groups in artificial urine. The siderophores enterobactin, aerobactin and yersiniabactin (P=0.016), with the iha and hlyA genes, may represent markers of increased urosepsis risk. Moreover, proteomic profiles between groups were significantly different (ferritin-1, iron uptake system component EfeO, ferrous iron transport protein B, nitrate/nitrite response regulator protein NarL, protein He...

Genes, Aug 22, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Fems Microbiology Letters, Apr 1, 1998

The ability of the IHF-like proteins of Acinetobacter junii and Proteus vulgaris to interact with... more The ability of the IHF-like proteins of Acinetobacter junii and Proteus vulgaris to interact with the HP attP and p P ihf sites of V DNA was investigated. IHF from A. junii and P. vulgaris was found to bind the examined ihf sites in a way similar to IHF from Escherichia coli as shown by gel mobility shift DNA binding assays and footprinting analysis. The three IHF proteins bound to the p P ihf site that overlaps the 335 region of that promoter and in vitro repression of transcription by each IHF was observed. These results confirm that IHF-like proteins from Gram-negative bacteria can recognize the same specific DNA sequences and appear to be important in regulation of transcription.

Cellular & Molecular Biology Letters, 2001

Postępy Mikrobiologii. Suplement, 2017

Medicina-lithuania, Jan 19, 2023

Postepy Mikrobiologii, 2007

Opisano najcześciej wykorzystywane techniki genotypowania w badaniach zakazen szpitalnych z uwzgl... more Opisano najcześciej wykorzystywane techniki genotypowania w badaniach zakazen szpitalnych z uwzglednieniem ich powtarzalności i potencjalu roznicującego oraz problemow technicznych i ponoszonych kosztow. Makrorestrykcyjna analiza genomowego DNA z wykorzystaniem PFGE (ang. Pulsed Field Gel Electrophoresis)jest uwazana za ''zloty standard'' w typowaniu molekularnym. Jednak jest ona czasochlonna, pracochlonna i dlatego nie nadaje sie do rutynowej diagnostyki. Proponuje sie stosowanie metod alternatywnych takich jak RAPD, AFLP, ADSRRS.

Journal of Clinical Microbiology, Aug 1, 2004

Clinical Microbiology and Infection, 2007

Journal of Applied Genetics, Mar 21, 2017

In patients with haematological malignancies, the bowel remains the main source of Escherichia co... more In patients with haematological malignancies, the bowel remains the main source of Escherichia coli bloodstream infections. We present the clinical example of recurrent bowel-blood translocations of E. coli with the unique virulence characteristics in a 55-year-old male with the diagnosis of acute myeloid leukaemia. The virulent factors profile of examined strains confirmed that the coexistence of genes papC, sfa, usp and cnf1, encoding virulence factors, predisposes E. coli to translocation from the gastrointestinal tract to the vascular bed. The close cooperation between haematologists and microbiologists is essential to improve the outcome of patients colonised with highly pathogenic strains.

Postepy Mikrobiologii, 2000

European Journal of Clinical Microbiology & Infectious Diseases, 2013

An analysis of the phylogenetic distribution and virulence genes of Escherichia coli isolates whi... more An analysis of the phylogenetic distribution and virulence genes of Escherichia coli isolates which predispose this bacteria to translocate from the urinary tract to the bloodstream is presented. One-dimensional analysis indicated that the occurrence of P fimbriae and α-hemolysin coding genes is more frequent among the E. coli which cause bacteremia. However, a two-dimensional analysis revealed that a combination of genes coding two adherence factors, namely, P + Dr, P + S, S + Dr, S + fim, and hemolysin + one adherence factor, were associated with bacteremia and, therefore, with the risk of translocation to the vascular system. The frequent and previously unrecognized coexistence of pro-inflammatory P fimbriae with the invasion promoting Dr adhesin in the same E. coli isolate may represent high-risk and potentially lethal pathogens. Escherichia coli, the predominant facultative organism of the intestinal flora, can cause severe extra-intestinal infections, including infection of the kidney (pyelonephritis) or bloodstream (bacteremia). When it escapes from its usual habitat, E. coli can colonize the genital tract and, as a subsequent step, ascend to the bladder and kidneys. Ascending urinary tract infection (UTI) is well explained by tissue receptor-E. coli adhesins interactions. Several E. coli virulence factors, including toxins, are implicated in renal inflammatory injury and

American Journal of Infection Control, 2014

Extended-spectrum β-lactamase (ESBL)-producing Klebsiella species cause worldwide problems in neo... more Extended-spectrum β-lactamase (ESBL)-producing Klebsiella species cause worldwide problems in neonatal intensive care units (NICUs). This study aimed to determine possible risk factors for infection or colonization with ESBL-producing Klebsiella pneumoniae (ESBLKp) during an outbreak in the NICU. A retrospective cohort study was conducted among neonates admitted to the NICU of a teaching hospital in Riyadh, Saudi Arabia, during an outbreak of ESBLKp from April to July 2008. The incidence density ratio was calculated to determine possible predictors of ESBLKp colonization or infection. During 2,265 person-days of follow-up of 118 neonates, 4 became infected, and 8 were colonized with ESBLKp. Univariate analyzes revealed that, among 14 neonates who were treated with vancomycin, 9 (64.3%) developed infection or colonization with ESBLKp, whereas, among 104 neonates who were not treated with vancomycin, 3 (2.9%) were affected, with an incidence density ratio of 4.22 (95% confidence interval: 1.47-5.15). Parenteral feeding and mechanical ventilation were found to be marginally significant risk factors. Treatment with vancomycin appears to be a risk factor for infection or colonization with ESBLKp in the NICU setting.

This article cites 13 articles, 9 of which can be accessed free at:

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Nephrology Dialysis Transplantation, 2016

(A) the 3′ recA-ddLMS PCR MaeII/Rsa</i... more (A) the 3′ recA-ddLMS PCR MaeII/RsaI, (B) PCR-RFLP/TspI typing of Acinetobacter calcoaceticus-baumannii complex. Ab1-Ab6 – A. baumannii strains; Ac1-Ac2 – A. calcoaceticus strains; Ap – A. pittii; An – A. nosocomialis; 1/3 – Acinetobacter gs. “Between 1 and 3”; C-to-13TU - Acinetobacter gs. “Close-to 13 TU” (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115181#pone-0115181-t001" target="_blank">Table 1</a>). M2- the molecular DNA size marker (100-3000 bp); M3 - the molecular DNA size marker (pUC19/MspI); K-, the negative control (without DNA).</p