Bienvenu BADOU - Academia.edu (original) (raw)
Papers by Bienvenu BADOU
Open Journal of Applied Sciences, 2023
Unavailability of performant planting material of pineapple constitutes a major problem of its c... more Unavailability of performant planting material of pineapple constitutes a major problem of its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the in vitro responses of two cultivars of
pineapple during the explant’s regeneration. Calli were induced from crown
leaf and plantlets leaf of “Smooth Cayenne” and “Sugarloaf cultivars”. Murashige and Skoog medium with vitamins B5 supplemented with different
growth regulators combinations were used. BAP and/or 2,4-D have been
added to base medium for calli cells’ differentiation while BAP and GA3 have
been added for plant elongation. The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II)
sulphate 5-hydrate concentrations incorporated had significant (p < 0.0001)
influence on callus induction in pineapple cultivars. Likewise, MS medium
with NAA (0.5 mg/l) + BAP (1 mg/l) had a highly significant influence with
8.8 differentiated Calli. Also, MS medium supplemented with BAP (3 mg/l) +
GA3 (2 mg/l) for the “smooth Cayenne” had significantly influenced (p <
0.0001) Calli regeneration with a high rate of 55.25% plantlets. MS medium
containing 0.5 mg/l of NAA + 0 mg/l IBA produced a high number of roots
in Sugarloaf whereas the medium containing 1.5 mg/l NAA + 0.5 mg/l (IBA)
produced high number of roots in smooth Cayenne. We have established an
efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect organogenesis. This protocol may be
used in genetics engineering studies for pineapple breeding.
Commercial Scale Tissue Culture for Horticulture and Plantation Crops, 2022
Advances in Bioscience and Biotechnology, 2022
Tissue culture techniques are widely used for the mass propagation of many species. In cashew in ... more Tissue culture techniques are widely used for the mass propagation of many species. In cashew in vitro propagation, some protocols need to be established at this end. The present work was carried out to evaluate the conditions for in vitro regeneration of cashew seedlings from micropropagation by organogenesis on Benin genotypes. Nodal explants from one-month-old cashew seedlings in the greenhouse and cotyledonary nodes from in vitro germination were used for this purpose. BAP and kinetin were evaluated alone at 2.2 mg/L and then the combination of 2.2 mg/L BAP + 0.2 mg/L IBA was also evaluated. The response of axillary bud proliferation on explants was obtained with both cotyledonary nodes and axillary buds from different combinations of growth regulators. However, the best responses were recorded with cotyledonary nodes. When 2.2 mg/L BAP was used, 80% of the explants responded with numerous proliferation (5 to 8) buds (5.75 ± 0.12) with good shoot length (6.73 ± 0.3 cm) on MS medium containing 150 mL coconut water. Rooting was observed with the combination of NAA (2.5 mg/l) + IBA (2.5 mg/l) on ½ MS containing 40 g/l sucrose.
Biotechnology Journal International, 2021
Aims: This work aims to evaluate the influence of different growth regulators combined with antio... more Aims: This work aims to evaluate the influence of different growth regulators combined with antioxidants on the induction of callus with different explants in cashew. Materials and Methods: Nucellar tissues, cotyledons and testa from elite tree nuts are cultured on media that differ in their concentration of 6-Benzylaminopurine (BAP: 0 mg/l; 0.25 mg/l; 0.5 mg/l) and Acid 2,4 dichlorophenoxyacetic (2,4-D: 0 mg/l; 0.8 mg/l). Therefore, for the control of the browning of the explants, the effect of activated charcoal and polyvinylpyrrolidone was tested. The amount of callus formed is evaluated after 28 days and after 90 days by simple observation according to a given scale. Results: Analysis of variance of callus formation 28 days after the culture of explants shows that the interaction between growth regulators and antioxidants significantly influences (p < 0.05) the induction of callus. The combination BAP 0.25mg/l and 2,4-D 0.8mg/l produces on average more callus (0.47 ± 0.00). ...
Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combinat... more Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus) In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple's homogeneous healthy planting materials.
However, the lack of plant material is the main factor that limits field extension. The aim of th... more However, the lack of plant material is the main factor that limits field extension. The aim of this work was to optimize the production of plant material for the two pineapple cultivars by tissue culture technique. Disinfection of the buds was evaluated by using antibiotics (gentamicin at 300 mg/l for 12 hours), and disinfectants (sodium hypochlorite and mercuric chloride) at different doses and immersion duration. Axillary buds of crown were seeded on Murashige and Skoog medium (MS) supplemented with agar (0.8%). Benzylaminopurine (BAP) was tested at different concentrations (5 mg/l and 10 mg/l) or combined with naphthalene acetic acid (NAA) (4.5 mg/l BAP + 0.7 mg/l NAA). Six to eight weeks after initiation, buds were transferred to MS medium supplemented with 1 mg/l BAP + 40 mg/l adenine sulfate. The lowest infection rate (26.07%) was recorded by 15% of Sodium hypochlorite when buds were immersed for 5 min. The best bud burst rate was obtained on BAP combined with NAA (65.09%) with Sugarloaf (64.95%). After the first subculture, an average of 12.70 and 15.30 shoots per plantlet were obtained respectively for Smooth Cayenne and Sugarloaf. The number of rooted plantlets and the length of roots varied depending on the cultivar.
Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combinat... more Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus) In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple's homogeneous healthy planting materials.
Journal of Applied Biosciences, Mar 26, 2018
Contrôle des germes endogènes par usage d'agents antimicrobiens et réponse de différents explants... more Contrôle des germes endogènes par usage d'agents antimicrobiens et réponse de différents explants durant la phase d'initiation in vitro de l'ananas (Ananas comosus (L.
Open Journal of Applied Sciences, 2023
Unavailability of performant planting material of pineapple constitutes a major problem of its c... more Unavailability of performant planting material of pineapple constitutes a major problem of its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the in vitro responses of two cultivars of
pineapple during the explant’s regeneration. Calli were induced from crown
leaf and plantlets leaf of “Smooth Cayenne” and “Sugarloaf cultivars”. Murashige and Skoog medium with vitamins B5 supplemented with different
growth regulators combinations were used. BAP and/or 2,4-D have been
added to base medium for calli cells’ differentiation while BAP and GA3 have
been added for plant elongation. The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II)
sulphate 5-hydrate concentrations incorporated had significant (p < 0.0001)
influence on callus induction in pineapple cultivars. Likewise, MS medium
with NAA (0.5 mg/l) + BAP (1 mg/l) had a highly significant influence with
8.8 differentiated Calli. Also, MS medium supplemented with BAP (3 mg/l) +
GA3 (2 mg/l) for the “smooth Cayenne” had significantly influenced (p <
0.0001) Calli regeneration with a high rate of 55.25% plantlets. MS medium
containing 0.5 mg/l of NAA + 0 mg/l IBA produced a high number of roots
in Sugarloaf whereas the medium containing 1.5 mg/l NAA + 0.5 mg/l (IBA)
produced high number of roots in smooth Cayenne. We have established an
efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect organogenesis. This protocol may be
used in genetics engineering studies for pineapple breeding.
Commercial Scale Tissue Culture for Horticulture and Plantation Crops, 2022
Advances in Bioscience and Biotechnology, 2022
Tissue culture techniques are widely used for the mass propagation of many species. In cashew in ... more Tissue culture techniques are widely used for the mass propagation of many species. In cashew in vitro propagation, some protocols need to be established at this end. The present work was carried out to evaluate the conditions for in vitro regeneration of cashew seedlings from micropropagation by organogenesis on Benin genotypes. Nodal explants from one-month-old cashew seedlings in the greenhouse and cotyledonary nodes from in vitro germination were used for this purpose. BAP and kinetin were evaluated alone at 2.2 mg/L and then the combination of 2.2 mg/L BAP + 0.2 mg/L IBA was also evaluated. The response of axillary bud proliferation on explants was obtained with both cotyledonary nodes and axillary buds from different combinations of growth regulators. However, the best responses were recorded with cotyledonary nodes. When 2.2 mg/L BAP was used, 80% of the explants responded with numerous proliferation (5 to 8) buds (5.75 ± 0.12) with good shoot length (6.73 ± 0.3 cm) on MS medium containing 150 mL coconut water. Rooting was observed with the combination of NAA (2.5 mg/l) + IBA (2.5 mg/l) on ½ MS containing 40 g/l sucrose.
Biotechnology Journal International, 2021
Aims: This work aims to evaluate the influence of different growth regulators combined with antio... more Aims: This work aims to evaluate the influence of different growth regulators combined with antioxidants on the induction of callus with different explants in cashew. Materials and Methods: Nucellar tissues, cotyledons and testa from elite tree nuts are cultured on media that differ in their concentration of 6-Benzylaminopurine (BAP: 0 mg/l; 0.25 mg/l; 0.5 mg/l) and Acid 2,4 dichlorophenoxyacetic (2,4-D: 0 mg/l; 0.8 mg/l). Therefore, for the control of the browning of the explants, the effect of activated charcoal and polyvinylpyrrolidone was tested. The amount of callus formed is evaluated after 28 days and after 90 days by simple observation according to a given scale. Results: Analysis of variance of callus formation 28 days after the culture of explants shows that the interaction between growth regulators and antioxidants significantly influences (p < 0.05) the induction of callus. The combination BAP 0.25mg/l and 2,4-D 0.8mg/l produces on average more callus (0.47 ± 0.00). ...
Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combinat... more Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus) In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple's homogeneous healthy planting materials.
However, the lack of plant material is the main factor that limits field extension. The aim of th... more However, the lack of plant material is the main factor that limits field extension. The aim of this work was to optimize the production of plant material for the two pineapple cultivars by tissue culture technique. Disinfection of the buds was evaluated by using antibiotics (gentamicin at 300 mg/l for 12 hours), and disinfectants (sodium hypochlorite and mercuric chloride) at different doses and immersion duration. Axillary buds of crown were seeded on Murashige and Skoog medium (MS) supplemented with agar (0.8%). Benzylaminopurine (BAP) was tested at different concentrations (5 mg/l and 10 mg/l) or combined with naphthalene acetic acid (NAA) (4.5 mg/l BAP + 0.7 mg/l NAA). Six to eight weeks after initiation, buds were transferred to MS medium supplemented with 1 mg/l BAP + 40 mg/l adenine sulfate. The lowest infection rate (26.07%) was recorded by 15% of Sodium hypochlorite when buds were immersed for 5 min. The best bud burst rate was obtained on BAP combined with NAA (65.09%) with Sugarloaf (64.95%). After the first subculture, an average of 12.70 and 15.30 shoots per plantlet were obtained respectively for Smooth Cayenne and Sugarloaf. The number of rooted plantlets and the length of roots varied depending on the cultivar.
Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combinat... more Research Article AJBB (2018) 2:9 Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus) In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple's homogeneous healthy planting materials.
Journal of Applied Biosciences, Mar 26, 2018
Contrôle des germes endogènes par usage d'agents antimicrobiens et réponse de différents explants... more Contrôle des germes endogènes par usage d'agents antimicrobiens et réponse de différents explants durant la phase d'initiation in vitro de l'ananas (Ananas comosus (L.