Charles Muscoplat - Academia.edu (original) (raw)

Papers by Charles Muscoplat

Journal of the American Veterinary Medical Association, Jan 15, 1980

The conditions under which the anthelmintic drug levamisole modulates immune function were review... more The conditions under which the anthelmintic drug levamisole modulates immune function were reviewed. Treatment with the drug corrects defective leukocyte reactivity and can bring clinical improvement in chronic infections, inflammatory disease, and malignancy. Therapeutic effectiveness depends upon dose, timing, and the patient's immune status, and results are often inconsistent. Levamisole therapy can cause marked but reversible complications, many of which have immunopathologic features.

Veterinary Immunology and Immunopathology, 1981

Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral... more Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [3H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 micrograms/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 micrograms/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle. B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.

International Archives of Allergy and Immunology, 1976

All rights, including that of translation into other languages, reserved. Photomechanic reproduct... more All rights, including that of translation into other languages, reserved. Photomechanic reproduction (photocopy, microcopy) of this volume or parts there of without special permission of the publishers is pro hibited.

Infection and Immunity, 1977

Anti-sheep erythrocyte antiserum suppressed the in vitro immune response of swine spleen cells to... more Anti-sheep erythrocyte antiserum suppressed the in vitro immune response of swine spleen cells to sheep erythrocytes. Inhibition of the in vitro anti-sheep erythrocyte response was also observed in neonatal spleen cells, which were obtained from piglets recently fed colostrum. Addition of purified protein derivative (PPD) to spleen cell cultures prepared from animals previously sensitized to Mycobacterium bovis reversed the immunosuppression caused by passive antibody. Passive antibody regulation was dose dependent; low doses of anti-sheep erythrocyte antiserum caused enhancement, whereas higher doses resulted in complete inhibition of anti-sheep erythrocyte responses. Addition of PPD to cultures of sensitized cells containing passive antibody resulted in progressively increased antibody responses as the dose of anti-sheep erythrocyte antibody increased. PPD addition also reversed maternal antibody suppression of in vitro 2-week-old neonatal spleen cell responses providing these ani...

PubMed, 1977

In summary, two large families were subjected to genetic analysis of the distribution of the immu... more In summary, two large families were subjected to genetic analysis of the distribution of the immune response. S linkage analysis indicated that this locus for positive skin test reaction to antigen E and/or personal history or seasonal allergy is linked to HLA-B. The maximum likelihood estimate of the recombination frequency is 0.13 in males and 0.53 in females, with a lod score of 2.53.

PubMed, Mar 1, 1980

Enumeration of bovine monocytes was determined by latex ingestion, using live cell and fixed cell... more Enumeration of bovine monocytes was determined by latex ingestion, using live cell and fixed cell techniques. Approximately 7.33% of the mononuclear leukocytes were found to phagocytize latex particles by live-cell technique and approximately 7.38% of mononuclear leukocytes phagocytized latex particles by fixed-cell technique. Lidocaine (30 mM) significantly inhibited phagocytosis of latex particles; however, the effects of lidocaine can be reversible. Adherent monocytes can be removed with lidocaine treatment and can be enriched to as much as 90% pure monocytes. Removal of lidocaine was associated with a return to normal cell function.

PubMed, Sep 1, 1999

Sjögren's syndrome (SS) is greatly under recognized in clinical practice, primarily for 2 reasons... more Sjögren's syndrome (SS) is greatly under recognized in clinical practice, primarily for 2 reasons: its presentations are variable and often nonspecific and there are still no clear, uniform diagnostic criteria for this clinical entity. The prevalence, natural history, pathogenesis, and clinical taxonomy of SS are still not well understood. Potential criteria include both subjective symptoms and objective criteria such as measurements of salivary and tear flow, minor salivary gland biopsy, and an increasing variety of serological markers. Physicians often fail to appreciate the profound impact of SS on quality of life. Therefore, screening for SS should include questions exploring symptoms in terms of their effect on the patient's daily life. At present, there is no curative treatment for SS. For symptom relief, local treatments (such as artificial tears or oral topical sprays) are limited in their effects, whereas systemic treatment offers the advantage of addressing a wider range of symptoms. Controlled studies show that oral pilocarpine significantly improves sicca symptoms in the eyes, mouth, and other sites. Clinical experience to date suggests it is safe and well tolerated, with no serious adverse effects, tachyphylaxis, or drug to drug interactions of concern. The most frequent adverse effects are sweating, urinary frequency, diarrhea, and other parasympathomimetic effects, but these do not lead to substantial drug withdrawal rates. Patients should be forewarned that subjective improvement may lag behind improvement in objective measures. Because management often spans several specialties, coordination among them is essential. Dental, gynecological, and ophthalmological perspectives on diagnosis and management are discussed; the primary practitioner has the opportunity to play both a coordinating role and a direct role in early diagnosis and treatment.

Journal of Nutrition and Food Sciences, Oct 30, 2014

PubMed, Mar 1, 1984

A hybridoma (6C6) producing antibodies with specificities to canine thymocyte surface antigens wa... more A hybridoma (6C6) producing antibodies with specificities to canine thymocyte surface antigens was produced using standard fusion techniques. The Aby 6C6 has affinity for medullary thymocytes, bone marrow cells, T- and B-peripheral lymphocytes, and monocytes. The Aby 6C6 appears to have 2 heavy chains with different molecular weights, an isoelectric focusing pattern consistent with monoclonality, and is an immunoglobulin G2b antibody with a kappa light chain.

American Association of Bovine Practitioners Conference Proceedings

Two major developments in biotechnology, namely recombinant DNA and hybridoma technology, have be... more Two major developments in biotechnology, namely recombinant DNA and hybridoma technology, have been employed to address problems in animal health and production. In this article we review three means by which these technologies have been employed to improve animal production: 1) prevent losses from infectious disease by facilitating the development of efficacious vaccines and antitoxins; 2) increase growth and milk production by providing unlimited quantities of genetically-engineered animal growth promotants; and 3) increase the nutritional quality of animal feed. This paper will first review the basic features of these new biotechnological developments and then discuss the kinds of reagents that have been developed by these technologies and how they will benefit animal health and production in the future.

Composition pharmaceutique, sous forme de comprimes, comprenant: - du chlorhydrate de pilocarpine... more Composition pharmaceutique, sous forme de comprimes, comprenant: - du chlorhydrate de pilocarpine, - de l'acide stearique, et - de la cellulose microcristalline. Ces comprimes peuvent etre utilises pour traiter les xerostomies resultant de radiotherapies de cancers du cou ou de la tete.

Agro Food Industry Hi-tech, 2015

Minnesota journal of law, science & technology, 2004

Intellectual property in agricultural biotechnology has spurred vigorous public debate over the g... more Intellectual property in agricultural biotechnology has spurred vigorous public debate over the global impact of advanced research in the life sciences. The University of Minnesota is not only a significant source of cutting-edge research, it is also a neutral forum for public debate. As the host of an April 29, 2004 conference, called “Intellectual Property Rights for the Public Good: Obligations of U.S. Universities to Developing Countries,” the University of Minnesota has taken the lead in examining the economic, societal, and ethical implications of its agricultural research.1 Resolving debates over intellectual property in agricultural technology is more than just a theoretical exercise. The ability to feed a growing population may rest on ensuring that the developing world receives and adopts new technology. Sharing that technology is a complex matter that involves social justice and academic ethics. My education in intellectual property and the development of genetic engineer...

Journal of Animal Science, 1985

Infection and Immunity, 1983

A monoclonal antibody (MCA) to enterotoxigenic Escherichia coli K99 antigen agglutinated K99+ ent... more A monoclonal antibody (MCA) to enterotoxigenic Escherichia coli K99 antigen agglutinated K99+ enterotoxigenic E. coli strains B44 (O9:K30;K99;F41:H-) and B41 (O101:K99;F41:H-) grown at 37 degrees C but not at 18 degrees C. The MCA, which was characterized as immunoglobulin G1, reacted specifically with K99 antigen in an enzyme-linked immunosorbent assay and precipitated radiolabeled K99 antigen. A total of 45 colostrum-fed and colostrum-deprived calves were used in three separate trials to determine whether the orally administered K99-specific MCA would prevent diarrhea caused by strain B44. Twenty-eight calves were fed 1 ml of mouse ascitic fluid containing K99-specific MCA at 10 h of age and were orally challenged with strain B44 at 12 to 14 h of age. Control calves either received no placebo or were fed 1 ml of mouse ascitic fluid containing fibronectin-specific MCA at 10 h of age. There was no difference in the incidence of diarrhea between the two groups after challenge. Howeve...

Journal of Clinical Case Reports, 2014

Infection and Immunity, 1980

To study the role of pulmonary alveolar macrophages (PAMs) in phagocytizing Pasteurella hemolytic... more To study the role of pulmonary alveolar macrophages (PAMs) in phagocytizing Pasteurella hemolytica , we developed an in vitro cultivation method for preparing them. This procedure provided an adherent monolayer of PAMs which were nonspecific esterase-positive and phagocytized latex beads. The phagocytosis and fate of P. hemolytica (biotype A, serotype 1) by PAMs in suspension were studied. The kinetics of phagocytosis were determined by quantitatively measuring the uptake of 24-h [ 3 H]thymidine-labeled bacteria by the PAMs in the presence of opsonins. Results showed that the uptake of P. hemolytica was enhanced in the presence of normal serum or antiserum. A total of 90% of the bacteria were phagocytized in the presence of normal adult bovine serum, and up to 95% were phagocytized in the presence of an antiserum. These studies also showed that normal serum, but not fetal calf serum, contained heat-stable natural antibodies which readily initiated the opsonization of P. hemolytica ....

JNCI: Journal of the National Cancer Institute, 1983

Individual Hereford cows bearing benign precursor lesions of ocular squamous cell carcinoma were ... more Individual Hereford cows bearing benign precursor lesions of ocular squamous cell carcinoma were treated by intralesional injection of mycobacterial cell walls in an oil-in-water emulsion in an attempt to interrupt neoplastic progression. Thirty-one months after treatment, statistical analysis of data indicated that intralesional BCG cell wall vaccine can interrupt this process and provides effective immunoprophylactic prevention of malignant disease.

Canadian journal of comparative medicine. Revue canadienne de médecine comparée, 1979

A study on cell-mediated immune responses in cattle with different exposure experiences to Brucel... more A study on cell-mediated immune responses in cattle with different exposure experiences to Brucella abortus was conducted by an in vitro lymphocyte stimulation assay. The purpose of this study was to determine how soon the cell-mediated immune responses would be detected following experimental exposure to B. abortus and to study the cell-mediated immune trend following experimental and natural exposure of cattle to B. abortus. The first positive cell-mediated immune responses occurred one to two weeks after experimental inoculation with living B. abortus strain 2308. The cell-mediated immune responses in these animals appeared at least one week before the appearance of of B. abortus serum agglutinating antibodies. Animals which were naturally infected with B. abortus biotypes 1 and 2 demonstrated positive cell-mediated immune responses throughout the study.