Chitta Patra - Academia.edu (original) (raw)
Papers by Chitta Patra
Advanced NanoBiomed Research
Inorganic nanoparticles (NPs) have been used for cancer theranostics application for last several... more Inorganic nanoparticles (NPs) have been used for cancer theranostics application for last several years. [1-3] Among them, noble metal NPs (gold, silver, and platinum NPs [PtNPs]) offer promising opportunity for various biomedical applications, including drug delivery, bioimaging, diagnostics, etc., owing to their distinctive physicochemical properties. [4-8] However, serious toxicological consequences might be observed on interaction of these NPs with the biological moieties at cellular and subcellular level. Based on this, several studies investigated potential toxicity of various nanomaterials, such as carbon nanotubes, graphene oxides, zinc oxides, cerium oxides, quantum dots, and silica NPs. [9-11] In this context, assessment of probable health risks associated with biologically important novel nanomaterials is essential for the safe biomedical application. Recently, PtNPs have gained vast attention in the field of healthcare and medicine. [5,12-17] However, exposure of PtNPs in a biological system might be associated with unanticipated hazards. [18-20] There are numerous reports on nephrotoxicity, hepatotoxicity, and inflammatory responses along with DNA damage induced by Ptbased NPs. [21,22] On the other hand, PtNPs are also reported to inhibit pulmonary inflammation by exerting antioxidants and free radical scavenging activity. [23-28] These conflicting results specify that the biological effects and toxicological behavior of PtNPs remain poorly explained. In our earlier report, we designed a cancer drug delivery system (DDS) using pegylated (polyethylene glycol functionalized
Potassium ferricyanide K 3 [Fe(CN) 6) ], copper sulfate(CuSO 4), manganese chloride (MnCl 2), nic... more Potassium ferricyanide K 3 [Fe(CN) 6) ], copper sulfate(CuSO 4), manganese chloride (MnCl 2), nickel acetate (Ni(OAc) 2), cobalt acetate (Co(OAc) 2), zinc nitrate (Zn(NO 3) 2), silver nitrate (AgNO 3), ferric nitrate (Fe(NO 3) 3), cadmium nitrate (Cd(NO 3) 2), RNase, propidium iodide (PI), Triton-X, penicillin, streptomycin, kanamycin, fetal bovine serum (FBS), MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide), Dulbecco phosphate buffer saline (DPBS), NBT/BCIP, sodium hydroxide (NaOH), hydrochloric acid (HCl), dimethyl sulfoxide (DMSO) and methanol (MeOH) were purchased from Sigma-Aldrich chemicals St. Louis MO, USA. Doxorubicin (Dox) was purchased from Alfa Aesar and used without further purification. A stock of solution of 5 µg/mL doxorubicin was prepared with Milli-Q water and used for the fabrication of nanoconjugates and cell culture experiments. All antibodies were purchased from Cell Signaling Technologies, USA. 1.2. Cell lines CHO: Hamster ovary cell line, A549: human lung cancer cells; SKOV3: human ovarian cancer cells and B16F10: mouse melanoma cell lines have been purchased from ATCC. HUVEC: human umbilical vein endothelial cells were purchased from Lonza. ECV-304 cells were a kind gift from Dr.
Glass Physics and Chemistry, 2006
Polycrystalline and nanocrystalline samples of the CuCr 2 Se 4 ferromagnetic spinel are synthesiz... more Polycrystalline and nanocrystalline samples of the CuCr 2 Se 4 ferromagnetic spinel are synthesized. The mean size of nanocrystals is 50 nm. The Curie temperature of spinel nanocrystals (465 K) is higher than that of spinel polycrystals (435 K). Laser irradiation at a wavelength of 1.5 µ m (in an external magnetic field) increases the magnetization of the noncrystalline powder. Most likely, this can be explained by the change in the orientation of magnetic moments of single-domain nanoparticles. The observed effect reaches a maximum in magnetic fields with a strength lower than that of the saturation field.
Journal of Materials Chemistry B, 2017
Overall schematic representation of the synthesis, characterization and proangiogenic activity of... more Overall schematic representation of the synthesis, characterization and proangiogenic activity of functionalized nanoceria.
Nanoscale, 2019
Shikimoyl-ligand decorated AuNPs for ex vivo engineered DC based DNA vaccination.
![Research paper thumbnail of Ag2[Fe(CN)5NO] Nanoparticles Exhibit Antibacterial Activity and Wound Healing Properties](https://attachments.academia-assets.com/101246440/thumbnails/1.jpg)
](ACS Biomaterials Science & Engineering, 2018
Therapeutic agents harboring both wound healing and antibacterial activities have much demand in ... more Therapeutic agents harboring both wound healing and antibacterial activities have much demand in biomedical applications. Development of such candidates with clinically approved materials adds more advantages towards these applications. Recently, silver metal complex nano-materials have been playing a major role in medical uses especially for anti-bacterial activity and wound healing. In this report, we designed and synthesized silver nitroprusside complex nanoparticles (abbreviated as AgNNPs) using sodium nitroprusside and silver nitrate (both are FDA approved precursors). The nanoparticles (AgNNPs) were thoroughly characterized by various physicochemical techniques such as XRD, FTIR, TGA, DLS, EDAX, Raman, ICP-OES, HRTEM and FESEM. The cell viability assay in normal cells (EA.hy 926 cells, NIH 3T3) using MTT reagents and CEA assay (CEA: Chick embryo angiogenesis assay) in fertilized eggs demonstrate the biocompatibility of AgNNPs. These nanoparticles show effective antibacterial activity against both Gram positive and Gram negative bacteria through membrane and DNA damage.
ACS Applied Bio Materials, 2019
Biomaterials Science, 2018
Gold nanoparticles functionalized with a thiol ligand containing both mannose mimicking shikimoyl... more Gold nanoparticles functionalized with a thiol ligand containing both mannose mimicking shikimoyl- and transfection enhancing guanidinyl-functionalities forin vivodelivery of DNA vaccines to dendritic cells.
Journal of Materials Chemistry B, 2017
PCL-EHNs scaffolds enhance endothelial cell proliferation, adhesion and blood vessel formation in... more PCL-EHNs scaffolds enhance endothelial cell proliferation, adhesion and blood vessel formation in a VEGFR2/Akt dependent signaling cascade.
Journal of Materials Chemistry B, 2017
Zinc oxide nanoflowers (ZONF) induce the generation of intracellular ROS that stimulates the phos... more Zinc oxide nanoflowers (ZONF) induce the generation of intracellular ROS that stimulates the phosphorylation of Akt and p38MAPK leading to activation of eNOS to produce NO, triggering angiogenesis in a cGMP dependent manner.
Biomaterials science, Jan 16, 2015
In the present study, we report the delivery of anti-cancer drug curcumin to cancer cells using m... more In the present study, we report the delivery of anti-cancer drug curcumin to cancer cells using mesoporous silica materials. A series of mesoporous silica material based drug delivery systems (S2, S4 and S6) were first designed and developed through the amine functionalization of KIT-6, MSU-2 and MCM-41 followed by the loading of curcumin. The curcumin loaded materials were characterized with several physico-chemical techniques and thoroughly screened on cancer cells to evaluate their in vitro drug delivery efficacy. All the curcumin loaded silica materials exhibited higher cellular uptake and inhibition of cancer cell viability compared to pristine curcumin. The effective internalization of curcumin in cancer cells through the mesoporous silica materials initiated the generation of intracellular reactive oxygen species and the down regulation of poly ADP ribose polymerase (PARP) enzyme levels compared to free curcumin leading to the activation of apoptosis. This study shows that th...
Journal of Materials Chemistry B, 2015
The synthesis and fabrication of green chemistry based biocompatible gold nanoparticles could be ... more The synthesis and fabrication of green chemistry based biocompatible gold nanoparticles could be clinically effective towards cancer therapeutics in the near future.
RSC Advances, 2015
A novel PDI-HIS probe detects Cu2+to form aggregated nonfluorescent complex. Addition of 0.58 ppm... more A novel PDI-HIS probe detects Cu2+to form aggregated nonfluorescent complex. Addition of 0.58 ppm ATP to this complex causes its rapid disaggregation thereby recovering the fluorescence by ∼99%in vitroand in A549 living cells.
Nanoporous Materials III, Proceedings of the 3rdInternational Symposium on Nanoporous Materials, 2002
The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution ... more The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution of bovine serum albumin (BSA) at room temperature. The processes were monitored by UV-Vis, circular dichroism, Raman and electron paramagnetic resonance (EPR) spectroscopies. TEM microscopy and dynamic light scattering (DLS) measurements were used to evidence changes in particle size during nanoparticle formation and growth. The formation of AuNPs at pH 7 in the absence of BSA was not observed, which proves that the albumin is involved in the first step of Au(III) reduction. Changes in the EPR spectral features of two spin probes, CAT16 and DIS3, with affinity for BSA and AuNPs, respectively, allowed us to monitor the particle growth and to demonstrate the protective role of BSA for AuNPs. The size of AuNPs formed in BSA solution increases slowly with time, resulting in nanoparticles of different morphologies, as revealed by TEM. Raman spectra of BSA indicate the interaction of albumin with AuNPs through sulfur-containing amino acid residues. This study shows that albumins act as both reducing agents and protective corona of AuNPs.
RSC Adv., 2014
A rhodamine–naphthalimde dyad capable of discerning Fe3+and Cr3+ions in aqueous samples is synthe... more A rhodamine–naphthalimde dyad capable of discerning Fe3+and Cr3+ions in aqueous samples is synthesized and its application in live cell imaging is demonstrated.
RSC Adv., 2014
The authors wish to amend the Acknowledgements section. The third sentence should read: Financial... more The authors wish to amend the Acknowledgements section. The third sentence should read: Financial support from CSIR 12 th Five Year Plan (FYP) projects CSC0114, CSC0302 and CSC0111 is acknowledged. The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.
PhysChemComm, 2001
There is tremendous current interest in the generation of nano-hybrid materials using silicate me... more There is tremendous current interest in the generation of nano-hybrid materials using silicate mesoporous materials. We describe herein a new process for the synthesis of gold nanoparticle-amine functionalized MCM-41 hybrid materials by the spontaneous reduction of chloroaurate ions within the silicate matrix. The gold nanoparticles thus formed are bound to the pores of the MCM-41 framework by amine functional groups and show excellent catalytic activity in hydrogenation reactions.
Journal of Nanobiotechnology, 2013
After the publication of this work [1], it was brought to our attention that there might have bee... more After the publication of this work [1], it was brought to our attention that there might have been an error during compilation of the gel bands in Figure 1. We regret such unintentional errors and any inconvenience this may have caused. In order to ensure that the published figure is correct, we have repeated the experiments to provide a corrected version of Figure 1.
Advanced NanoBiomed Research
Inorganic nanoparticles (NPs) have been used for cancer theranostics application for last several... more Inorganic nanoparticles (NPs) have been used for cancer theranostics application for last several years. [1-3] Among them, noble metal NPs (gold, silver, and platinum NPs [PtNPs]) offer promising opportunity for various biomedical applications, including drug delivery, bioimaging, diagnostics, etc., owing to their distinctive physicochemical properties. [4-8] However, serious toxicological consequences might be observed on interaction of these NPs with the biological moieties at cellular and subcellular level. Based on this, several studies investigated potential toxicity of various nanomaterials, such as carbon nanotubes, graphene oxides, zinc oxides, cerium oxides, quantum dots, and silica NPs. [9-11] In this context, assessment of probable health risks associated with biologically important novel nanomaterials is essential for the safe biomedical application. Recently, PtNPs have gained vast attention in the field of healthcare and medicine. [5,12-17] However, exposure of PtNPs in a biological system might be associated with unanticipated hazards. [18-20] There are numerous reports on nephrotoxicity, hepatotoxicity, and inflammatory responses along with DNA damage induced by Ptbased NPs. [21,22] On the other hand, PtNPs are also reported to inhibit pulmonary inflammation by exerting antioxidants and free radical scavenging activity. [23-28] These conflicting results specify that the biological effects and toxicological behavior of PtNPs remain poorly explained. In our earlier report, we designed a cancer drug delivery system (DDS) using pegylated (polyethylene glycol functionalized
Potassium ferricyanide K 3 [Fe(CN) 6) ], copper sulfate(CuSO 4), manganese chloride (MnCl 2), nic... more Potassium ferricyanide K 3 [Fe(CN) 6) ], copper sulfate(CuSO 4), manganese chloride (MnCl 2), nickel acetate (Ni(OAc) 2), cobalt acetate (Co(OAc) 2), zinc nitrate (Zn(NO 3) 2), silver nitrate (AgNO 3), ferric nitrate (Fe(NO 3) 3), cadmium nitrate (Cd(NO 3) 2), RNase, propidium iodide (PI), Triton-X, penicillin, streptomycin, kanamycin, fetal bovine serum (FBS), MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide), Dulbecco phosphate buffer saline (DPBS), NBT/BCIP, sodium hydroxide (NaOH), hydrochloric acid (HCl), dimethyl sulfoxide (DMSO) and methanol (MeOH) were purchased from Sigma-Aldrich chemicals St. Louis MO, USA. Doxorubicin (Dox) was purchased from Alfa Aesar and used without further purification. A stock of solution of 5 µg/mL doxorubicin was prepared with Milli-Q water and used for the fabrication of nanoconjugates and cell culture experiments. All antibodies were purchased from Cell Signaling Technologies, USA. 1.2. Cell lines CHO: Hamster ovary cell line, A549: human lung cancer cells; SKOV3: human ovarian cancer cells and B16F10: mouse melanoma cell lines have been purchased from ATCC. HUVEC: human umbilical vein endothelial cells were purchased from Lonza. ECV-304 cells were a kind gift from Dr.
Glass Physics and Chemistry, 2006
Polycrystalline and nanocrystalline samples of the CuCr 2 Se 4 ferromagnetic spinel are synthesiz... more Polycrystalline and nanocrystalline samples of the CuCr 2 Se 4 ferromagnetic spinel are synthesized. The mean size of nanocrystals is 50 nm. The Curie temperature of spinel nanocrystals (465 K) is higher than that of spinel polycrystals (435 K). Laser irradiation at a wavelength of 1.5 µ m (in an external magnetic field) increases the magnetization of the noncrystalline powder. Most likely, this can be explained by the change in the orientation of magnetic moments of single-domain nanoparticles. The observed effect reaches a maximum in magnetic fields with a strength lower than that of the saturation field.
Journal of Materials Chemistry B, 2017
Overall schematic representation of the synthesis, characterization and proangiogenic activity of... more Overall schematic representation of the synthesis, characterization and proangiogenic activity of functionalized nanoceria.
Nanoscale, 2019
Shikimoyl-ligand decorated AuNPs for ex vivo engineered DC based DNA vaccination.
ACS Biomaterials Science & Engineering, 2018
Therapeutic agents harboring both wound healing and antibacterial activities have much demand in ... more Therapeutic agents harboring both wound healing and antibacterial activities have much demand in biomedical applications. Development of such candidates with clinically approved materials adds more advantages towards these applications. Recently, silver metal complex nano-materials have been playing a major role in medical uses especially for anti-bacterial activity and wound healing. In this report, we designed and synthesized silver nitroprusside complex nanoparticles (abbreviated as AgNNPs) using sodium nitroprusside and silver nitrate (both are FDA approved precursors). The nanoparticles (AgNNPs) were thoroughly characterized by various physicochemical techniques such as XRD, FTIR, TGA, DLS, EDAX, Raman, ICP-OES, HRTEM and FESEM. The cell viability assay in normal cells (EA.hy 926 cells, NIH 3T3) using MTT reagents and CEA assay (CEA: Chick embryo angiogenesis assay) in fertilized eggs demonstrate the biocompatibility of AgNNPs. These nanoparticles show effective antibacterial activity against both Gram positive and Gram negative bacteria through membrane and DNA damage.
ACS Applied Bio Materials, 2019
Biomaterials Science, 2018
Gold nanoparticles functionalized with a thiol ligand containing both mannose mimicking shikimoyl... more Gold nanoparticles functionalized with a thiol ligand containing both mannose mimicking shikimoyl- and transfection enhancing guanidinyl-functionalities forin vivodelivery of DNA vaccines to dendritic cells.
Journal of Materials Chemistry B, 2017
PCL-EHNs scaffolds enhance endothelial cell proliferation, adhesion and blood vessel formation in... more PCL-EHNs scaffolds enhance endothelial cell proliferation, adhesion and blood vessel formation in a VEGFR2/Akt dependent signaling cascade.
Journal of Materials Chemistry B, 2017
Zinc oxide nanoflowers (ZONF) induce the generation of intracellular ROS that stimulates the phos... more Zinc oxide nanoflowers (ZONF) induce the generation of intracellular ROS that stimulates the phosphorylation of Akt and p38MAPK leading to activation of eNOS to produce NO, triggering angiogenesis in a cGMP dependent manner.
Biomaterials science, Jan 16, 2015
In the present study, we report the delivery of anti-cancer drug curcumin to cancer cells using m... more In the present study, we report the delivery of anti-cancer drug curcumin to cancer cells using mesoporous silica materials. A series of mesoporous silica material based drug delivery systems (S2, S4 and S6) were first designed and developed through the amine functionalization of KIT-6, MSU-2 and MCM-41 followed by the loading of curcumin. The curcumin loaded materials were characterized with several physico-chemical techniques and thoroughly screened on cancer cells to evaluate their in vitro drug delivery efficacy. All the curcumin loaded silica materials exhibited higher cellular uptake and inhibition of cancer cell viability compared to pristine curcumin. The effective internalization of curcumin in cancer cells through the mesoporous silica materials initiated the generation of intracellular reactive oxygen species and the down regulation of poly ADP ribose polymerase (PARP) enzyme levels compared to free curcumin leading to the activation of apoptosis. This study shows that th...
Journal of Materials Chemistry B, 2015
The synthesis and fabrication of green chemistry based biocompatible gold nanoparticles could be ... more The synthesis and fabrication of green chemistry based biocompatible gold nanoparticles could be clinically effective towards cancer therapeutics in the near future.
RSC Advances, 2015
A novel PDI-HIS probe detects Cu2+to form aggregated nonfluorescent complex. Addition of 0.58 ppm... more A novel PDI-HIS probe detects Cu2+to form aggregated nonfluorescent complex. Addition of 0.58 ppm ATP to this complex causes its rapid disaggregation thereby recovering the fluorescence by ∼99%in vitroand in A549 living cells.
Nanoporous Materials III, Proceedings of the 3rdInternational Symposium on Nanoporous Materials, 2002
The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution ... more The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution of bovine serum albumin (BSA) at room temperature. The processes were monitored by UV-Vis, circular dichroism, Raman and electron paramagnetic resonance (EPR) spectroscopies. TEM microscopy and dynamic light scattering (DLS) measurements were used to evidence changes in particle size during nanoparticle formation and growth. The formation of AuNPs at pH 7 in the absence of BSA was not observed, which proves that the albumin is involved in the first step of Au(III) reduction. Changes in the EPR spectral features of two spin probes, CAT16 and DIS3, with affinity for BSA and AuNPs, respectively, allowed us to monitor the particle growth and to demonstrate the protective role of BSA for AuNPs. The size of AuNPs formed in BSA solution increases slowly with time, resulting in nanoparticles of different morphologies, as revealed by TEM. Raman spectra of BSA indicate the interaction of albumin with AuNPs through sulfur-containing amino acid residues. This study shows that albumins act as both reducing agents and protective corona of AuNPs.
RSC Adv., 2014
A rhodamine–naphthalimde dyad capable of discerning Fe3+and Cr3+ions in aqueous samples is synthe... more A rhodamine–naphthalimde dyad capable of discerning Fe3+and Cr3+ions in aqueous samples is synthesized and its application in live cell imaging is demonstrated.
RSC Adv., 2014
The authors wish to amend the Acknowledgements section. The third sentence should read: Financial... more The authors wish to amend the Acknowledgements section. The third sentence should read: Financial support from CSIR 12 th Five Year Plan (FYP) projects CSC0114, CSC0302 and CSC0111 is acknowledged. The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.
PhysChemComm, 2001
There is tremendous current interest in the generation of nano-hybrid materials using silicate me... more There is tremendous current interest in the generation of nano-hybrid materials using silicate mesoporous materials. We describe herein a new process for the synthesis of gold nanoparticle-amine functionalized MCM-41 hybrid materials by the spontaneous reduction of chloroaurate ions within the silicate matrix. The gold nanoparticles thus formed are bound to the pores of the MCM-41 framework by amine functional groups and show excellent catalytic activity in hydrogenation reactions.
Journal of Nanobiotechnology, 2013
After the publication of this work [1], it was brought to our attention that there might have bee... more After the publication of this work [1], it was brought to our attention that there might have been an error during compilation of the gel bands in Figure 1. We regret such unintentional errors and any inconvenience this may have caused. In order to ensure that the published figure is correct, we have repeated the experiments to provide a corrected version of Figure 1.