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Papers by Christine Archimbaud

Research paper thumbnail of Repeated genomic transfers from echovirus 30 to echovirus 6 lineages indicate co-divergence between co-circulating populations of the two human enterovirus serotypes

Infection, Genetics and Evolution, Mar 1, 2011

Research paper thumbnail of Coxsackievirus A6 Recombinant Subclades D3/A and D3/H Were Predominant in Hand-Foot-And-Mouth Disease Outbreaks in the Paediatric Population, France, 2010–2018

Research paper thumbnail of Prospective analysis of 61 cases of enteroviral meningitis: interest of systematic genome detection in cerebrospinal fluid irrespective of cytologic examination results

Journal of Clinical Virology, Apr 1, 2001

Research paper thumbnail of Emerging and reemerging enterovirus diseases: From poliomyelitis to hand, foot and mouth disease

PubMed, Apr 1, 2014

Several picornaviruses (Picornaviridae) are currently attracting interest without the need of bei... more Several picornaviruses (Picornaviridae) are currently attracting interest without the need of being "emergent". The Parechovirus genus, validated 40 years after the discovery of the first two members ("echoviruses 22 and 23") includes neurotropic viruses whose molecular diagnosis demonstrated the involvement in infant meningitis and newborn sepsis, in particular type 3. Improvements in multiplex molecular diagnosis of respiratory infections - thanks to the Influenza AH1N1pdm2009 pandemy - showed that rhinoviruses may be involved in severe forms. The risk of the re-emergence of poliomyelitis in Europe, after an 11-year period of elimination, is a serious threat, owing to the circulation of the wild-type poliovirus in the Middle East and Africa because of conflicts, population displacements and poverty. The current widespread epidemics of hand-foot-mouth disease and/or meningitis infections due to enterovirus 71, with fatal encephalitis and cardio-pulmonary failure, are clear evidence of its emergence in South-East Asia. Although uncommon in Europe and less frequently incriminated than coxsackieviruses A6 and A10 in hand-foot-mouth disease, EV71 represents a real risk for the future. Extensive genotyping of the enteroviruses by the Enterovirus Surveillance Network should ward off these two potential risks of emergence/reemergence.

Research paper thumbnail of Comparative T and B immune responses of four different anti-COVID-19 vaccine strategies 6 months after vaccination

Journal of Infection, May 1, 2022

Research paper thumbnail of Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective observational study

Clinical Microbiology and Infection, May 1, 2012

Research paper thumbnail of Molecular Evidence of Persistent Echovirus 13 Meningoencephalitis in a Patient with Relapsed Lymphoma after an Outbreak of Meningitis in 2000

Journal of Clinical Microbiology, Oct 1, 2003

Research paper thumbnail of Variations in Cerebrospinal Fluid Viral Loads Among Enterovirus Genotypes in Patients Hospitalized With Laboratory-Confirmed Meningitis Due to Enterovirus

The Journal of Infectious Diseases, Mar 20, 2014

Research paper thumbnail of Ambulatory Pediatric Surveillance of Hand, Foot and Mouth Disease as Signal of an Outbreak of Coxsackievirus A6 Infections, France, 2014–2015

Emerging Infectious Diseases, Nov 1, 2016

Research paper thumbnail of Pneumonie fatale à adénovirus type 3 chez un adulte immunocompétent

Medecine Et Maladies Infectieuses, Feb 1, 2004

Research paper thumbnail of Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid

Journal of Clinical Microbiology, 2008

Research paper thumbnail of Enterovirus genotyping directly from original clinical specimens: Prospective application to a severe neonatal infection

Journal of Clinical Virology, Feb 1, 2009

Research paper thumbnail of Diagnostic rapide des infections à rotavirus : étude prospective comparative de deux techniques de détection d'antigènes dans les selles

Pathologie Biologie, Jul 1, 2006

Les performances de deux trousses de détection rapide de l'antigène rotavirus ont été comparées s... more Les performances de deux trousses de détection rapide de l'antigène rotavirus ont été comparées sur des selles reçues au laboratoire de virologie du CHU de Clermont-Ferrand entre septembre 2002 et mai 2003. Cinq cent douze selles ont été analysées par la trousse immunochromatographique Diarlex ® MB (ICG) et par la trousse immunoenzymatique IDEIA TM Rotavirus (EIA). Les échantillons donnant des résultats discordants ont été observés en microscopie électronique (ME) et les dossiers cliniques correspondants réexaminés. Sur 512 échantillons, 155 (30,3 %) étaient positifs et 332 (64,8 %) négatifs avec les deux trousses. Les résultats étaient contradictoires dans 25 cas (4,88 %), soient 24 enfants et un adulte, la trousse EIA donnant davantage de résultats positifs. L'examen en ME, possible rétrospectivement pour 15/25 échantillons discordants, a confirmé la présence de rotavirus dans 7/14 selles positives seulement en EIA, et un échantillon positif seulement en ICG. Le réexamen des 25 observations cliniques a montré la présence de signes de GEA dans tous les cas. L'analyse statistique montre une concordance excellente entre les deux trousses (kappa = 0,89, IC 95 % = [0,85-0,93]) malgré un sous-dépistage du test ICG par rapport au test EIA (p < 0,0001).

Research paper thumbnail of Enterovirus meningitis in Tunisia (Monastir, Mahdia, 2011–2013): identification of virus variants cocirculating in France

Diagnostic Microbiology and Infectious Disease, Feb 1, 2016

Acute enterovirus (EV) meningitis is a frequent cause of hospitalisation, and over 100 EV serotyp... more Acute enterovirus (EV) meningitis is a frequent cause of hospitalisation, and over 100 EV serotypes may be involved. A total of 215 patients of all ages with meningitis signs were investigated in 2 Tunisian hospitals. Their cerebrospinal fluid (CSF) was analysed retrospectively for EVs with a TaqMan real-time RT-qPCR. The virus strains were typed, and their evolutionary relationships were determined by Bayesian phylogenetic methods. An EV genome was detected in 21/215 patients (9.8%). The CSF viral loads ranged from 3.27 to 5.63 log10 genome copies/mL. The strains were identified in 13/21 patients and assigned to EV-B types. Viruses identified in Tunisian patients were genetically related to variants detected in France. The viral loads were similar in Tunisian and French patients for most EV types. The phylogenetic data and viral loads determined in Tunisian and French patients suggest that close EV variants were involved in aseptic meningitis in the 2 countries over a same period.

Research paper thumbnail of Emergence of recent echovirus 30 lineages is marked by serial genetic recombination events

Journal of General Virology, 2007

Research paper thumbnail of Phylogeography of circulating populations of human echovirus 30 over 50 years: Nucleotide polymorphism and signature of purifying selection in the VP1 capsid protein gene

Infection, Genetics and Evolution, Jul 1, 2009

A comprehensive set of 443 1D gene sequences (encoding the VP1 capsid protein) was analyzed to in... more A comprehensive set of 443 1D gene sequences (encoding the VP1 capsid protein) was analyzed to investigate the phylogenetic relationships and evolutionary patterns among strains of human echovirus 30 (E30; genus Enterovirus, family Picornaviridae) characterized over 50 years. Maximum-likelihood (ML) phylogenetic trees of complete and nonredundant 1D gene sequences (total length=876 nucleotides) showed evidence of distinct lineages related to the isolation period of virus strains. Virus transportation was confirmed as a major epidemiological factor in the appearance of epidemics since recurrence of aseptic meningitis outbreaks in a given geographic area was associated with distinct E30 variants detected earlier in distant regions. Detection of the codon changes associated with E30 evolution was investigated with methods implemented in the Datamonkey web server. Evolution of the 1D gene was dominated by continual negative (purifying) selection against nonsynonymous substitutions at most codon sites, as determined by dN/dS ratio. Amino acid polymorphism was maintained at a limited number of sites (10/292) in the VP1 protein (within loops connecting beta strands and C-terminus). Amino acid changes are allowed at these sites because they are likely exposed on the virion particle and nonsynonymous substitutions are observed in the corresponding codons because negative selection is relaxed.

Research paper thumbnail of In vitro adhesive properties and virulence factors of Enterococcus faecalis strains

Research in Microbiology, Mar 1, 2002

Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from ... more Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from stools of healthy volunteers were investigated for their ability to adhere to Int-407 and Girardi heart cell lines and for the presence of known enterococcal virulence factors. Eight strains (27.6%) adhered predominantly to Int-407 cells. The adherence of enterococci was enhanced by proteolytic digestion, suggesting that some cell binding components become surface-exposed after treatment with trypsin. The occurrence of known potential virulence factors of enterococci among these strains was determined and was as follows: enterococcal surface protein (72.4%), gelatinase (58.6%), aggregation substance (48.3%) and cytolysin (17.2%). Bacterial adherence was not significantly associated with any of these virulence factors.  2002 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

Research paper thumbnail of Interaction of enterovirus A71 with the human blood-brain barrier: an in vitro study

HAL (Le Centre pour la Communication Scientifique Directe), Nov 25, 2022

Research paper thumbnail of A large-scale outbreak of hand, foot and mouth disease, France, as at 28 September 2021

Eurosurveillance, Oct 28, 2021

Research paper thumbnail of Phylogeography of Coxsackievirus A16 Reveals Global Transmission Pathways and Recent Emergence and Spread of a Recombinant Genogroup

Journal of Virology, Sep 15, 2017

Research paper thumbnail of Repeated genomic transfers from echovirus 30 to echovirus 6 lineages indicate co-divergence between co-circulating populations of the two human enterovirus serotypes

Infection, Genetics and Evolution, Mar 1, 2011

Research paper thumbnail of Coxsackievirus A6 Recombinant Subclades D3/A and D3/H Were Predominant in Hand-Foot-And-Mouth Disease Outbreaks in the Paediatric Population, France, 2010–2018

Research paper thumbnail of Prospective analysis of 61 cases of enteroviral meningitis: interest of systematic genome detection in cerebrospinal fluid irrespective of cytologic examination results

Journal of Clinical Virology, Apr 1, 2001

Research paper thumbnail of Emerging and reemerging enterovirus diseases: From poliomyelitis to hand, foot and mouth disease

PubMed, Apr 1, 2014

Several picornaviruses (Picornaviridae) are currently attracting interest without the need of bei... more Several picornaviruses (Picornaviridae) are currently attracting interest without the need of being "emergent". The Parechovirus genus, validated 40 years after the discovery of the first two members ("echoviruses 22 and 23") includes neurotropic viruses whose molecular diagnosis demonstrated the involvement in infant meningitis and newborn sepsis, in particular type 3. Improvements in multiplex molecular diagnosis of respiratory infections - thanks to the Influenza AH1N1pdm2009 pandemy - showed that rhinoviruses may be involved in severe forms. The risk of the re-emergence of poliomyelitis in Europe, after an 11-year period of elimination, is a serious threat, owing to the circulation of the wild-type poliovirus in the Middle East and Africa because of conflicts, population displacements and poverty. The current widespread epidemics of hand-foot-mouth disease and/or meningitis infections due to enterovirus 71, with fatal encephalitis and cardio-pulmonary failure, are clear evidence of its emergence in South-East Asia. Although uncommon in Europe and less frequently incriminated than coxsackieviruses A6 and A10 in hand-foot-mouth disease, EV71 represents a real risk for the future. Extensive genotyping of the enteroviruses by the Enterovirus Surveillance Network should ward off these two potential risks of emergence/reemergence.

Research paper thumbnail of Comparative T and B immune responses of four different anti-COVID-19 vaccine strategies 6 months after vaccination

Journal of Infection, May 1, 2022

Research paper thumbnail of Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective observational study

Clinical Microbiology and Infection, May 1, 2012

Research paper thumbnail of Molecular Evidence of Persistent Echovirus 13 Meningoencephalitis in a Patient with Relapsed Lymphoma after an Outbreak of Meningitis in 2000

Journal of Clinical Microbiology, Oct 1, 2003

Research paper thumbnail of Variations in Cerebrospinal Fluid Viral Loads Among Enterovirus Genotypes in Patients Hospitalized With Laboratory-Confirmed Meningitis Due to Enterovirus

The Journal of Infectious Diseases, Mar 20, 2014

Research paper thumbnail of Ambulatory Pediatric Surveillance of Hand, Foot and Mouth Disease as Signal of an Outbreak of Coxsackievirus A6 Infections, France, 2014–2015

Emerging Infectious Diseases, Nov 1, 2016

Research paper thumbnail of Pneumonie fatale à adénovirus type 3 chez un adulte immunocompétent

Medecine Et Maladies Infectieuses, Feb 1, 2004

Research paper thumbnail of Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid

Journal of Clinical Microbiology, 2008

Research paper thumbnail of Enterovirus genotyping directly from original clinical specimens: Prospective application to a severe neonatal infection

Journal of Clinical Virology, Feb 1, 2009

Research paper thumbnail of Diagnostic rapide des infections à rotavirus : étude prospective comparative de deux techniques de détection d'antigènes dans les selles

Pathologie Biologie, Jul 1, 2006

Les performances de deux trousses de détection rapide de l'antigène rotavirus ont été comparées s... more Les performances de deux trousses de détection rapide de l'antigène rotavirus ont été comparées sur des selles reçues au laboratoire de virologie du CHU de Clermont-Ferrand entre septembre 2002 et mai 2003. Cinq cent douze selles ont été analysées par la trousse immunochromatographique Diarlex ® MB (ICG) et par la trousse immunoenzymatique IDEIA TM Rotavirus (EIA). Les échantillons donnant des résultats discordants ont été observés en microscopie électronique (ME) et les dossiers cliniques correspondants réexaminés. Sur 512 échantillons, 155 (30,3 %) étaient positifs et 332 (64,8 %) négatifs avec les deux trousses. Les résultats étaient contradictoires dans 25 cas (4,88 %), soient 24 enfants et un adulte, la trousse EIA donnant davantage de résultats positifs. L'examen en ME, possible rétrospectivement pour 15/25 échantillons discordants, a confirmé la présence de rotavirus dans 7/14 selles positives seulement en EIA, et un échantillon positif seulement en ICG. Le réexamen des 25 observations cliniques a montré la présence de signes de GEA dans tous les cas. L'analyse statistique montre une concordance excellente entre les deux trousses (kappa = 0,89, IC 95 % = [0,85-0,93]) malgré un sous-dépistage du test ICG par rapport au test EIA (p < 0,0001).

Research paper thumbnail of Enterovirus meningitis in Tunisia (Monastir, Mahdia, 2011–2013): identification of virus variants cocirculating in France

Diagnostic Microbiology and Infectious Disease, Feb 1, 2016

Acute enterovirus (EV) meningitis is a frequent cause of hospitalisation, and over 100 EV serotyp... more Acute enterovirus (EV) meningitis is a frequent cause of hospitalisation, and over 100 EV serotypes may be involved. A total of 215 patients of all ages with meningitis signs were investigated in 2 Tunisian hospitals. Their cerebrospinal fluid (CSF) was analysed retrospectively for EVs with a TaqMan real-time RT-qPCR. The virus strains were typed, and their evolutionary relationships were determined by Bayesian phylogenetic methods. An EV genome was detected in 21/215 patients (9.8%). The CSF viral loads ranged from 3.27 to 5.63 log10 genome copies/mL. The strains were identified in 13/21 patients and assigned to EV-B types. Viruses identified in Tunisian patients were genetically related to variants detected in France. The viral loads were similar in Tunisian and French patients for most EV types. The phylogenetic data and viral loads determined in Tunisian and French patients suggest that close EV variants were involved in aseptic meningitis in the 2 countries over a same period.

Research paper thumbnail of Emergence of recent echovirus 30 lineages is marked by serial genetic recombination events

Journal of General Virology, 2007

Research paper thumbnail of Phylogeography of circulating populations of human echovirus 30 over 50 years: Nucleotide polymorphism and signature of purifying selection in the VP1 capsid protein gene

Infection, Genetics and Evolution, Jul 1, 2009

A comprehensive set of 443 1D gene sequences (encoding the VP1 capsid protein) was analyzed to in... more A comprehensive set of 443 1D gene sequences (encoding the VP1 capsid protein) was analyzed to investigate the phylogenetic relationships and evolutionary patterns among strains of human echovirus 30 (E30; genus Enterovirus, family Picornaviridae) characterized over 50 years. Maximum-likelihood (ML) phylogenetic trees of complete and nonredundant 1D gene sequences (total length=876 nucleotides) showed evidence of distinct lineages related to the isolation period of virus strains. Virus transportation was confirmed as a major epidemiological factor in the appearance of epidemics since recurrence of aseptic meningitis outbreaks in a given geographic area was associated with distinct E30 variants detected earlier in distant regions. Detection of the codon changes associated with E30 evolution was investigated with methods implemented in the Datamonkey web server. Evolution of the 1D gene was dominated by continual negative (purifying) selection against nonsynonymous substitutions at most codon sites, as determined by dN/dS ratio. Amino acid polymorphism was maintained at a limited number of sites (10/292) in the VP1 protein (within loops connecting beta strands and C-terminus). Amino acid changes are allowed at these sites because they are likely exposed on the virion particle and nonsynonymous substitutions are observed in the corresponding codons because negative selection is relaxed.

Research paper thumbnail of In vitro adhesive properties and virulence factors of Enterococcus faecalis strains

Research in Microbiology, Mar 1, 2002

Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from ... more Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from stools of healthy volunteers were investigated for their ability to adhere to Int-407 and Girardi heart cell lines and for the presence of known enterococcal virulence factors. Eight strains (27.6%) adhered predominantly to Int-407 cells. The adherence of enterococci was enhanced by proteolytic digestion, suggesting that some cell binding components become surface-exposed after treatment with trypsin. The occurrence of known potential virulence factors of enterococci among these strains was determined and was as follows: enterococcal surface protein (72.4%), gelatinase (58.6%), aggregation substance (48.3%) and cytolysin (17.2%). Bacterial adherence was not significantly associated with any of these virulence factors.  2002 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

Research paper thumbnail of Interaction of enterovirus A71 with the human blood-brain barrier: an in vitro study

HAL (Le Centre pour la Communication Scientifique Directe), Nov 25, 2022

Research paper thumbnail of A large-scale outbreak of hand, foot and mouth disease, France, as at 28 September 2021

Eurosurveillance, Oct 28, 2021

Research paper thumbnail of Phylogeography of Coxsackievirus A16 Reveals Global Transmission Pathways and Recent Emergence and Spread of a Recombinant Genogroup

Journal of Virology, Sep 15, 2017