D. Pamfil - Academia.edu (original) (raw)

Papers by D. Pamfil

The glycine-rich RNA-binding proteins of Arabidopsis thaliana are of growing interest in the cont... more The glycine-rich RNA-binding proteins of Arabidopsis thaliana are of growing interest in the context of understanding how plants respond to biotic and abiotic stresses. In this study we focus on the phenotypic analysis of Arabidopsis knock-out mutants of genes that encode the glycine-rich proteins atGRP2 and atGRP7, comparing them to knock-out mutants of ABI3, ABI4 and ABI5, genes linked to cell signaling through the plant hormone abscisic acid (ABA). The results show that the abscisic acid insensitive (ABI) mutants developed faster than wild-type plants. In contrast the glycine-rich protein knock-out mutants (grp7-1 and grp2) did not grow as well, suggesting an involvement of these genes in key early developmental processes. There was a significant difference in the phenotype of the knockout mutants grp2 and grp7-1, suggesting that atGRP7 and atGRP2 have overlapping yet distinct roles in development.

HortScience, 1996

RAPD analysis was performed on 44 species of Rubus. These species included representatives from s... more RAPD analysis was performed on 44 species of Rubus. These species included representatives from seven of the 12 subgenera within the genus and several series within the Idaeobatus (raspberries) and Eubatus (blackberries) subgenera. For all species, up to five plants were initially analyzed by two 10-mer primers. The most heterozygous of these individuals was then analyzed using 13 additional primers. Wide band diversity exists among Rubus species; these differences were analyzed using the PHYLIP software program. These differences are repeatable, for example color sports of `Heritage' red raspberry produced identical banding patterns. The genetic similarity between eastern United States blackberries (Eubatus) and numerous species was compared to the ability of these same species to act as a suitable pollen parent for eastern blackberries. These data were used to construct a dendrogram of the subgenera studied here.

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2009

The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to expl... more The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to explore its relation to population size. We surveyed two populations of G. nivalis found in Oriental Carpathians, by RAPD markers. Four decamer primers of arbitrary sequence were used in order to asses genetic variability within and between the two populations. Our studies revealed two different levels of genetic variability within populations in relation with the grazing policies. The mean genetic distances within the population found in overgrazed grassland decreased significantly in comparison with that growing in ungrazed grassland. Therefore, this method can by successfully used to asses genetic variability within and between Gentiana populations

Abstract: The regeneration through meristem culture is an advanced biotechnological tehnique whic... more Abstract: The regeneration through meristem culture is an advanced biotechnological tehnique which is a very useful and valuable method and represents a key in the fruit stock material production chain. In the modern fruit planting material production system and in the pathogen elimination systems it occupies a central place. In this article we will present the main aspects regarding the behavior of some plum cultivars in the process of the in vitro meristem culture: initiation, multiplication, acclimation. It is well known the fact that the propagation of woody fruit species and in general that of stone fruit species is difficult through tissue cultures and especially the propagation systems through meristems. For initation there has been used four variants concerning two nutritive media and two apex sizes, one of 0.2-0.8 mm. The culture media was MS medium ( Murashige and Skoog, 1967) with two different hormone concentration 0,7 mg/l BAP and 2 mg/l BAP.The biological material used...

sa.agr.hr

The purpose of our study was testing some markers for the resistance genes to Tilletia spp. and u... more The purpose of our study was testing some markers for the resistance genes to Tilletia spp. and using these markers in the selection for resistance. In order to do this, we have tested, at the F2 generation, RAPD markers and microsatellite markers (SSR) known ...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 2011

Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a b... more Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a breeding program. Our objective was to investigate the level of genetic diversity among maize inbred lines. Eighty-three maize inbred lines obtained from SCDA Turda were genotyped using 20 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 17 bands (OPA 03) and 7 bands (OPAB 11). The highest numbers of polymorphic bands were obtained with primer OPA 03, respectively 17 bands, followed by OPA 01, OPB 08 (16 polymorphic bands) and OPX 03 and OPAL 20 (13 polymorphic bands). Genetic distances were established using Nei Li/Dice coefficent and an UPGMA dendrogram was constructed with FreeTree software. The built dendrogram shows phylogenetic relationships between the analysed biological material.

Trees habit is an important trait in pear breeding and pear culture (1, 2, 3). The segregation fo... more Trees habit is an important trait in pear breeding and pear culture (1, 2, 3). The segregation for trees habit of F1 pear hybrids belonging from six combinations was analysed. The seedlings originate in incomplete diallel cross, effectuated with four genitors (Comtesse de Paris, Milenium, Virgiliu Hibernal, Cluj 89-1-81 selection), were framed in six classes of growth (after UPOV Guidelines), respectively architectural ideotype: 1. Fastigiate; 2. Upright;

JOURNAL OF PLANT PATHOLOGY

SUMMARY The serological and genetic variability of Plum pox virus (PPV) isolates from transgenic ... more SUMMARY The serological and genetic variability of Plum pox virus (PPV) isolates from transgenic plum trees expressing the PPV coat protein gene and conventional plums was analyzed. PPV isolates were characterized serologically by TAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies and by molecular typing across three genomic regions as well as RFLP analysis of the 3’ terminus of the CP gene. PCR products spanning the (Cter)CP and (Cter)NIb-(Nter)CP regions were sequenced. Sequence information revealed no significant difference between serological and molecular features of PPV isolates from transgenic and conventional plums that were growing together in experimental orchards for 6-8 years. These results show that transgenic plums do not promote the emergence of new PPV variants under field conditions.

Romanian …, 2010

The genetic diversity of 51 accessions belonging to Juglans genus maintained in the Romanian Nati... more The genetic diversity of 51 accessions belonging to Juglans genus maintained in the Romanian National Collection at the Station for Research and Development for Fruit Growth Valcea (S.C.D.P. Valcea) was assessed, using RAPD (Random Amplified Polymorphic DNA) markers. The 25 primers used in this study yielded scorable amplification patterns. The produced fingerprint distinguished the identical accessions, confirming their genetic identity and discriminated all the other accessions. Accessions representing Romanian homologated cultivars tend to group together according to their origin. The determined genetic variability was specific to a germplasm collection and to the high number of different accessions studied. The RAPD markers can be useful in developing DNA fingerprinting techniques to distinguish the valuable genotypes used in selection.

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2008

Proper identification of Castanea, Corylus, Juglans and Prunus accessions is needed to ensure tha... more Proper identification of Castanea, Corylus, Juglans and Prunus accessions is needed to ensure that the product is genetically pure to meet the expectations of growers and distributors and is also important in protecting the legal rights of breeders. The use of RAPD technique for molecular characterization of valuable genotypes represents a new approach in Romania. The 32 genotypes used in this study were obtained from the Castanea, Corylus, Juglans and Prunus collections maintained at S.C.D.P. Valcea. The potential use of RAPD technique for characterization and assessment of genetic relationships was investigated in Castanea, Corylus, Juglans and Prunus genotypes. Thirty seven of the 54 decamer primers yielded scorable amplification patterns. These primers generated polymorphic bands among the genotypes studied. Some of the primers produced no amplification or unreadable gel smears. The primers which generater the most polymorphic bands in Castanea genus were OPAB 11 and OPA 04, in ...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2018

The main aim of this study was to investigate the genetic diversity and phylogenetic relationship... more The main aim of this study was to investigate the genetic diversity and phylogenetic relationships of six Ranunculus species using SRAP markers. The genotyping of 73 Ranunculus accessions showed that 12 primer pairs generated a total number of 212 polymorphic fragments with a 99.53 % level of polymorphism. Nei’s gene diversity ranged between 0.2237 and 0.3436 with an average of 0.3001. The UPGMA method with Nei72’s similarity coefficient ranging from 0.23 to 1.11 separated 73 accessions into two distinct groups (A and B). Cluster-A included R. acris members, while cluster-B included the rest of the tested species. Cluster-B was subsequently divided into two sub-groups (I and II). Subgroup-I consisted of R. carpaticus and R. serpens subsp. nemorosus accessions, while subgroup-II included the members of R. platanifolius, R. polyanthemos, and R. repens. R. polyanthemos and R. repens were founded the most closely related species. These findings provide a brand-new understanding of the e...

Accurate identification of grapevine cultivars is important for Romanian wine- makers and the ext... more Accurate identification of grapevine cultivars is important for Romanian wine- makers and the extrapolation of the grapevine ident ification methods into musts is particularly releva nt in controlling the quality and authenticity of arom atic and high quality red wines. In this study, molecular SSR (Simple Sequence Repeats) analysis of Vitis vinifera L. cultivars ("Busuioac ă de Bohotin", "Tămâioas ă Româneasc ă", "Negru Aromat", "Feteasc ă Neagr ă", "Negru de Dr ăgăs ani", "Amurg" and "Novac") was applied in order to detect and correctly identify the grapevine cultivars present in monovarietal musts. DNA provided from each analyzed cultivar was successful extracted from leaves and must at the beginning of the fermen tation (day 1) using CTAB method and amplicons generated from eight SSR primers (VVS2, VVS5, VVS29, MD5, MD7, ZAG 47, ZAG 62, ZAG 79) were analyzed. Results shows that, at molecular lev el, there were...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2014

The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to expl... more The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to explore its relation to population size. We surveyed two populations of G. nivalis found in Oriental Carpathians, by RAPD markers. Four decamer primers of arbitrary sequence were used in order to asses genetic variability within and between the two populations. Our studies revealed two different levels of genetic variability within populations in relation with the grazing policies. The mean genetic distances within the population found in overgrazed grassland decreased significantly in comparison with that growing in ungrazed grassland. Therefore, this method can by successfully used to asses genetic variability within and between Gentiana populations.

Abstract. DNA fingerprinting was generated for two different Quercus robur L. embryogenic culture... more Abstract. DNA fingerprinting was generated for two different Quercus robur L. embryogenic culture lines (NL100- Quercus robur L., and Qf3- Quercus robur var. fastigiata) using random amplified polymorphic DNA (RAPD). A total of ten arbitrary oligonucleotide (decamer) primers were used to study variability in DNA sequences within and between the two embryogenic lines. Seven out of ten primers gave polymorphism between the two Quercus embryogenic lines, two primers gave no polymorphism between the embryogenic lines and one primer (OPAL 20) has shown intraclonal polymorphism (somaclonal variation) within the line NL 100. Therefore, this primer can be used to screen for somaclonal variation which could appear due to tissue culture condition. Primers which gave polymorphism between the two embryogenic lines allow the two different lines and possible other Quercus varieties or species to be distinguished.

World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 2011

An experimental study is presented on the effect of Conservation Agriculture (CA) compared to Con... more An experimental study is presented on the effect of Conservation Agriculture (CA) compared to Conventional Agriculture (ConvA) upon Maize Yield based on split-plot model. Two factors have been considered: A Factor-Fertilization with two variants: A1N40P40 kg/ha and A2N90P70 kg/ha; B FactorCrop protection with 4 variants : B14 treatments, B2-3 treatments, B32 treatments and B41 treatment. In comparison with conventional agriculture, CA determined lower maize yields. Fertilization is the key factor determining a yield gain of 973.58 kg/ha in ConvA and 1,123.33 kg/ha in CA. A reduced number of treatments determined a yield decline. The A-B interaction had a positive effect on maize yield when a larger amount of fertilizer and 4 or 3 treatments were applied in ConvA and a benefic in CA for highest fertilization level and 2 treatments. The B2A2 ConvA variant was the most efficient leading to 302.67 kg/ha gain while B3A2 CA variant brought 181.33 kg production gain. Keywords—conservation ...

Abstract. Scab, caused by Venturia inaequalis, is the most important apple disease worldwide, the... more Abstract. Scab, caused by Venturia inaequalis, is the most important apple disease worldwide, therefore all major apple breeding programs have included disease resistance as a primary goal. In Romania, especially in Transylvania climatic conditions are extremely favorable for disease development and spread. In addition, the vast majority of the apple cultivars in these areas are susceptible to the infection. In this study, we analyzed 64 apple plants (cultivars and hybrids) cultivated in Transylvania in order to genotype for the Vf locus. Plants were screened using specific DNA markers for the presence of Vf resistance gene.

The glycine-rich RNA-binding proteins of Arabidopsis thaliana are of growing interest in the cont... more The glycine-rich RNA-binding proteins of Arabidopsis thaliana are of growing interest in the context of understanding how plants respond to biotic and abiotic stresses. In this study we focus on the phenotypic analysis of Arabidopsis knock-out mutants of genes that encode the glycine-rich proteins atGRP2 and atGRP7, comparing them to knock-out mutants of ABI3, ABI4 and ABI5, genes linked to cell signaling through the plant hormone abscisic acid (ABA). The results show that the abscisic acid insensitive (ABI) mutants developed faster than wild-type plants. In contrast the glycine-rich protein knock-out mutants (grp7-1 and grp2) did not grow as well, suggesting an involvement of these genes in key early developmental processes. There was a significant difference in the phenotype of the knockout mutants grp2 and grp7-1, suggesting that atGRP7 and atGRP2 have overlapping yet distinct roles in development.

HortScience, 1996

RAPD analysis was performed on 44 species of Rubus. These species included representatives from s... more RAPD analysis was performed on 44 species of Rubus. These species included representatives from seven of the 12 subgenera within the genus and several series within the Idaeobatus (raspberries) and Eubatus (blackberries) subgenera. For all species, up to five plants were initially analyzed by two 10-mer primers. The most heterozygous of these individuals was then analyzed using 13 additional primers. Wide band diversity exists among Rubus species; these differences were analyzed using the PHYLIP software program. These differences are repeatable, for example color sports of `Heritage' red raspberry produced identical banding patterns. The genetic similarity between eastern United States blackberries (Eubatus) and numerous species was compared to the ability of these same species to act as a suitable pollen parent for eastern blackberries. These data were used to construct a dendrogram of the subgenera studied here.

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2009

The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to expl... more The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to explore its relation to population size. We surveyed two populations of G. nivalis found in Oriental Carpathians, by RAPD markers. Four decamer primers of arbitrary sequence were used in order to asses genetic variability within and between the two populations. Our studies revealed two different levels of genetic variability within populations in relation with the grazing policies. The mean genetic distances within the population found in overgrazed grassland decreased significantly in comparison with that growing in ungrazed grassland. Therefore, this method can by successfully used to asses genetic variability within and between Gentiana populations

Abstract: The regeneration through meristem culture is an advanced biotechnological tehnique whic... more Abstract: The regeneration through meristem culture is an advanced biotechnological tehnique which is a very useful and valuable method and represents a key in the fruit stock material production chain. In the modern fruit planting material production system and in the pathogen elimination systems it occupies a central place. In this article we will present the main aspects regarding the behavior of some plum cultivars in the process of the in vitro meristem culture: initiation, multiplication, acclimation. It is well known the fact that the propagation of woody fruit species and in general that of stone fruit species is difficult through tissue cultures and especially the propagation systems through meristems. For initation there has been used four variants concerning two nutritive media and two apex sizes, one of 0.2-0.8 mm. The culture media was MS medium ( Murashige and Skoog, 1967) with two different hormone concentration 0,7 mg/l BAP and 2 mg/l BAP.The biological material used...

sa.agr.hr

The purpose of our study was testing some markers for the resistance genes to Tilletia spp. and u... more The purpose of our study was testing some markers for the resistance genes to Tilletia spp. and using these markers in the selection for resistance. In order to do this, we have tested, at the F2 generation, RAPD markers and microsatellite markers (SSR) known ...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 2011

Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a b... more Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a breeding program. Our objective was to investigate the level of genetic diversity among maize inbred lines. Eighty-three maize inbred lines obtained from SCDA Turda were genotyped using 20 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 17 bands (OPA 03) and 7 bands (OPAB 11). The highest numbers of polymorphic bands were obtained with primer OPA 03, respectively 17 bands, followed by OPA 01, OPB 08 (16 polymorphic bands) and OPX 03 and OPAL 20 (13 polymorphic bands). Genetic distances were established using Nei Li/Dice coefficent and an UPGMA dendrogram was constructed with FreeTree software. The built dendrogram shows phylogenetic relationships between the analysed biological material.

Trees habit is an important trait in pear breeding and pear culture (1, 2, 3). The segregation fo... more Trees habit is an important trait in pear breeding and pear culture (1, 2, 3). The segregation for trees habit of F1 pear hybrids belonging from six combinations was analysed. The seedlings originate in incomplete diallel cross, effectuated with four genitors (Comtesse de Paris, Milenium, Virgiliu Hibernal, Cluj 89-1-81 selection), were framed in six classes of growth (after UPOV Guidelines), respectively architectural ideotype: 1. Fastigiate; 2. Upright;

JOURNAL OF PLANT PATHOLOGY

SUMMARY The serological and genetic variability of Plum pox virus (PPV) isolates from transgenic ... more SUMMARY The serological and genetic variability of Plum pox virus (PPV) isolates from transgenic plum trees expressing the PPV coat protein gene and conventional plums was analyzed. PPV isolates were characterized serologically by TAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies and by molecular typing across three genomic regions as well as RFLP analysis of the 3’ terminus of the CP gene. PCR products spanning the (Cter)CP and (Cter)NIb-(Nter)CP regions were sequenced. Sequence information revealed no significant difference between serological and molecular features of PPV isolates from transgenic and conventional plums that were growing together in experimental orchards for 6-8 years. These results show that transgenic plums do not promote the emergence of new PPV variants under field conditions.

Romanian …, 2010

The genetic diversity of 51 accessions belonging to Juglans genus maintained in the Romanian Nati... more The genetic diversity of 51 accessions belonging to Juglans genus maintained in the Romanian National Collection at the Station for Research and Development for Fruit Growth Valcea (S.C.D.P. Valcea) was assessed, using RAPD (Random Amplified Polymorphic DNA) markers. The 25 primers used in this study yielded scorable amplification patterns. The produced fingerprint distinguished the identical accessions, confirming their genetic identity and discriminated all the other accessions. Accessions representing Romanian homologated cultivars tend to group together according to their origin. The determined genetic variability was specific to a germplasm collection and to the high number of different accessions studied. The RAPD markers can be useful in developing DNA fingerprinting techniques to distinguish the valuable genotypes used in selection.

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2008

Proper identification of Castanea, Corylus, Juglans and Prunus accessions is needed to ensure tha... more Proper identification of Castanea, Corylus, Juglans and Prunus accessions is needed to ensure that the product is genetically pure to meet the expectations of growers and distributors and is also important in protecting the legal rights of breeders. The use of RAPD technique for molecular characterization of valuable genotypes represents a new approach in Romania. The 32 genotypes used in this study were obtained from the Castanea, Corylus, Juglans and Prunus collections maintained at S.C.D.P. Valcea. The potential use of RAPD technique for characterization and assessment of genetic relationships was investigated in Castanea, Corylus, Juglans and Prunus genotypes. Thirty seven of the 54 decamer primers yielded scorable amplification patterns. These primers generated polymorphic bands among the genotypes studied. Some of the primers produced no amplification or unreadable gel smears. The primers which generater the most polymorphic bands in Castanea genus were OPAB 11 and OPA 04, in ...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2018

The main aim of this study was to investigate the genetic diversity and phylogenetic relationship... more The main aim of this study was to investigate the genetic diversity and phylogenetic relationships of six Ranunculus species using SRAP markers. The genotyping of 73 Ranunculus accessions showed that 12 primer pairs generated a total number of 212 polymorphic fragments with a 99.53 % level of polymorphism. Nei’s gene diversity ranged between 0.2237 and 0.3436 with an average of 0.3001. The UPGMA method with Nei72’s similarity coefficient ranging from 0.23 to 1.11 separated 73 accessions into two distinct groups (A and B). Cluster-A included R. acris members, while cluster-B included the rest of the tested species. Cluster-B was subsequently divided into two sub-groups (I and II). Subgroup-I consisted of R. carpaticus and R. serpens subsp. nemorosus accessions, while subgroup-II included the members of R. platanifolius, R. polyanthemos, and R. repens. R. polyanthemos and R. repens were founded the most closely related species. These findings provide a brand-new understanding of the e...

Accurate identification of grapevine cultivars is important for Romanian wine- makers and the ext... more Accurate identification of grapevine cultivars is important for Romanian wine- makers and the extrapolation of the grapevine ident ification methods into musts is particularly releva nt in controlling the quality and authenticity of arom atic and high quality red wines. In this study, molecular SSR (Simple Sequence Repeats) analysis of Vitis vinifera L. cultivars ("Busuioac ă de Bohotin", "Tămâioas ă Româneasc ă", "Negru Aromat", "Feteasc ă Neagr ă", "Negru de Dr ăgăs ani", "Amurg" and "Novac") was applied in order to detect and correctly identify the grapevine cultivars present in monovarietal musts. DNA provided from each analyzed cultivar was successful extracted from leaves and must at the beginning of the fermen tation (day 1) using CTAB method and amplicons generated from eight SSR primers (VVS2, VVS5, VVS29, MD5, MD7, ZAG 47, ZAG 62, ZAG 79) were analyzed. Results shows that, at molecular lev el, there were...

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2014

The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to expl... more The amount of genetic variation in the rare annual herb Gentiana nivalis L was determined to explore its relation to population size. We surveyed two populations of G. nivalis found in Oriental Carpathians, by RAPD markers. Four decamer primers of arbitrary sequence were used in order to asses genetic variability within and between the two populations. Our studies revealed two different levels of genetic variability within populations in relation with the grazing policies. The mean genetic distances within the population found in overgrazed grassland decreased significantly in comparison with that growing in ungrazed grassland. Therefore, this method can by successfully used to asses genetic variability within and between Gentiana populations.

Abstract. DNA fingerprinting was generated for two different Quercus robur L. embryogenic culture... more Abstract. DNA fingerprinting was generated for two different Quercus robur L. embryogenic culture lines (NL100- Quercus robur L., and Qf3- Quercus robur var. fastigiata) using random amplified polymorphic DNA (RAPD). A total of ten arbitrary oligonucleotide (decamer) primers were used to study variability in DNA sequences within and between the two embryogenic lines. Seven out of ten primers gave polymorphism between the two Quercus embryogenic lines, two primers gave no polymorphism between the embryogenic lines and one primer (OPAL 20) has shown intraclonal polymorphism (somaclonal variation) within the line NL 100. Therefore, this primer can be used to screen for somaclonal variation which could appear due to tissue culture condition. Primers which gave polymorphism between the two embryogenic lines allow the two different lines and possible other Quercus varieties or species to be distinguished.

World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 2011

An experimental study is presented on the effect of Conservation Agriculture (CA) compared to Con... more An experimental study is presented on the effect of Conservation Agriculture (CA) compared to Conventional Agriculture (ConvA) upon Maize Yield based on split-plot model. Two factors have been considered: A Factor-Fertilization with two variants: A1N40P40 kg/ha and A2N90P70 kg/ha; B FactorCrop protection with 4 variants : B14 treatments, B2-3 treatments, B32 treatments and B41 treatment. In comparison with conventional agriculture, CA determined lower maize yields. Fertilization is the key factor determining a yield gain of 973.58 kg/ha in ConvA and 1,123.33 kg/ha in CA. A reduced number of treatments determined a yield decline. The A-B interaction had a positive effect on maize yield when a larger amount of fertilizer and 4 or 3 treatments were applied in ConvA and a benefic in CA for highest fertilization level and 2 treatments. The B2A2 ConvA variant was the most efficient leading to 302.67 kg/ha gain while B3A2 CA variant brought 181.33 kg production gain. Keywords—conservation ...

Abstract. Scab, caused by Venturia inaequalis, is the most important apple disease worldwide, the... more Abstract. Scab, caused by Venturia inaequalis, is the most important apple disease worldwide, therefore all major apple breeding programs have included disease resistance as a primary goal. In Romania, especially in Transylvania climatic conditions are extremely favorable for disease development and spread. In addition, the vast majority of the apple cultivars in these areas are susceptible to the infection. In this study, we analyzed 64 apple plants (cultivars and hybrids) cultivated in Transylvania in order to genotype for the Vf locus. Plants were screened using specific DNA markers for the presence of Vf resistance gene.