Dan Nonneman - Academia.edu (original) (raw)

Papers by Dan Nonneman

2013 Midwest Meeting (March 11-13, 2013), Mar 13, 2013

Verhandlungen, Oct 1, 2002

CRC Press eBooks, Nov 16, 2004

Molecular Reproduction and Development, Jun 15, 2022

Domestic Animal Endocrinology, Oct 1, 2020

Dna Sequence, Jul 1, 2003

Journal of Animal Science, May 1, 2021

Anestrus, or failure to express estrus during boar exposure, is commonly observed in replacement ... more Anestrus, or failure to express estrus during boar exposure, is commonly observed in replacement gilts, and results primarily from either delayed onset of puberty (prepubertal; PP) or cyclic ovulations without behavioral estrus (behavioral anestrus; BA). Gilts born between 2007 and 2018 at USMARC were observed for age at puberty between 160 and 240 days of age. Mature boars were placed in an alleyway pen while a herdsman observed gilts for standing lordosis in response to the back pressure test. Gilts failing to be observed in estrus by 240 days of age were slaughtered (250.4 ± 0.3 days of age) and reproductive tracts recovered to determine if gilts had ovulated. Gilts were defined as PP (n = 606), BA (n = 649), or Peripubertal (n = 118; PP with large preovulatory follicles on the ovary). There were 96 age-matched, cyclic contemporary gilts included as cyclic control gilts. Body weights were recorded at birth, weaning, 8 weeks, and 21 weeks of age with hot carcass weight (HCW) recorded at slaughter. The objective was to retrospectively determine if growth and HCW differed between these groups. Data were analyzed as a mixed ANOVA using group as a fixed effect with sire and farrowing group to which the gilt was born as random effects. Birth weight, weaning weight, ADG at weaning, and weight at 8 weeks did not differ between groups (P &gt; 0.16). The BA gilts had greater growth rate (weight per day of pig age at 21 weeks; P &lt; 0.02) and HCW (P &lt; 0.0001) than PP and Peripubertal gilts, which also had smaller HCW than control gilts. Some cyclic control gilts (7.3%) that displayed estrous behavior had a prepubertal reproductive tract with no ovulatory activity at slaughter. Results indicated that gilts exhibiting delayed puberty grow slower late in development and during boar exposure. Estrus without ovulation in replacement gilts may be more prevalent than assumed. USDA is an equal opportunity provider and employer.

Journal of the Endocrine Society, Apr 1, 2020

Journal of Animal Science, Jul 1, 2019

A significant proportion of gilts that enter the herd never farrow a litter and are culled becaus... more A significant proportion of gilts that enter the herd never farrow a litter and are culled because of anestrus or failure to conceive. Genome-wide association studies for pubertal traits have identified loci involved in neuronal and olfactory pathways, and olfaction is critical for expression of reproductive behavior in mammalian females. We evaluated major olfactory epithelium (MOE) for differential gene expression in nonpubertal, behavioral anestrus and normal cycling early follicular and luteal phase gilts (n = 8/group; average age of 259 days). An average of 50 million paired-end RNA-seq reads were collected from each of the 32 RNA libraries and mapped to Sscrofa 11.1. Differential gene expression was determined using DESeq2. A total of 18,484 genes were expressed with a mean normalized expression value greater than 5. Only four genes were differentially expressed between nonpubertal or behavioral anestrus MOE and their cycling controls (early follicular and luteal, respectively). Comparing cycling follicular and luteal phase gilts showed that 1146 genes were more highly expressed in MOE from follicular phase gilts, whereas 1351 genes were more highly expressed in MOE from luteal phase gilts. Pathways for transmembrane receptor protein tyrosine kinase/growth factor signaling, cell junction and epithelium development were overrepresented in follicular phase MOE and cell cycle, chromatin remodeling, inflammatory response and sensory perception were overrepresented in luteal phase MOE. While 1348 locus IDs were identified for olfactory receptors in MOE, only 160 were expressed at an appreciable level (base mean &amp;amp;gt; 5) in MOE and 16 were more highly expressed in MOE from luteal phase than follicular phase gilts. While few genes were differentially expressed in MOE between prepubertal and anestrus gilts and cycling gilts at the same ovarian stage, the comparison between ovarian stages indicates that MOE gene expression is under hormonal control. USDA is an equal opportunity provider and employer.

Journal of Animal Science, Sep 1, 2016

PTs assigned with HGNC gene symbols and Ensembl pig Gene IDs and tissue or cell types where their... more PTs assigned with HGNC gene symbols and Ensembl pig Gene IDs and tissue or cell types where their human counterparts specifically or preferentially expressed. (TXT 3006Â kb)

Additional file 2: Table S2. Genes containing loss-of function (LOF), nonsynonymous (NONSYN), syn... more Additional file 2: Table S2. Genes containing loss-of function (LOF), nonsynonymous (NONSYN), synonymous (SYN), regulatory (UTR), and other variants and the positions of variants.

Journal of Animal Science

Journal of Animal Science, Jan 29, 2018

Genomic regions, SNP results and candidate genes associated with age at puberty in pigs. (XLSX 37... more Genomic regions, SNP results and candidate genes associated with age at puberty in pigs. (XLSX 37Â kb)

Distribution of GO terms related to immune system process. (PDF 36Â kb)