Dana Ashirbekova - Academia.edu (original) (raw)
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Université Sorbonne Paris Nord / Sorbonne Paris Nord University
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Papers by Dana Ashirbekova
Nucleic Acids Research, 1991
Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as co... more Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical recommendations are given for selection of the condensing reagent depending on the properties of the duplex. Based on 31 P NMR spectroscopy data, a scheme is suggested for BrCN activation of the nucleotide phosphomonoester group. Using both condensing reagents, we studied the condensation of oligonucleotides containing ribo-segments (from mononucleotide residue to full sequence) on the DNA template. Efficiency of the chemical ligation of RNA oligomers was shown to be much lower than that of DNA analogues. The coupling yield depends on the position of the RNA segment in the hybrid duplexes and on the position of the phosphate group in the nick.
FEBS Letters, 1988
Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in a... more Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes. Cyanogen bromide; Chemical ligation; Modified DNA duplex; Intemucleotide bond
Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as co... more Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical recommendations are given for selection of the condensing reagent depending on the properties of the duplex. Based on 31 P NMR spectroscopy data, a scheme is suggested for BrCN activation of the nucleotide phosphomonoester group. Using both condensing reagents, we studied the condensation of oligonucleotides containing ribo-segments (from mononucleotide residue to full sequence) on the DNA template. Efficiency of the chemical ligation of RNA oligomers was shown to be much lower than that of DNA analogues. The coupling yield depends on the position of the RNA segment in the hybrid duplexes and on the position of the phosphate group in the nick.
Nucleic Acids Research, 1991
Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as co... more Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical recommendations are given for selection of the condensing reagent depending on the properties of the duplex. Based on 31 P NMR spectroscopy data, a scheme is suggested for BrCN activation of the nucleotide phosphomonoester group. Using both condensing reagents, we studied the condensation of oligonucleotides containing ribo-segments (from mononucleotide residue to full sequence) on the DNA template. Efficiency of the chemical ligation of RNA oligomers was shown to be much lower than that of DNA analogues. The coupling yield depends on the position of the RNA segment in the hybrid duplexes and on the position of the phosphate group in the nick.
FEBS Letters, 1988
Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in a... more Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes. Cyanogen bromide; Chemical ligation; Modified DNA duplex; Intemucleotide bond
Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as co... more Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical recommendations are given for selection of the condensing reagent depending on the properties of the duplex. Based on 31 P NMR spectroscopy data, a scheme is suggested for BrCN activation of the nucleotide phosphomonoester group. Using both condensing reagents, we studied the condensation of oligonucleotides containing ribo-segments (from mononucleotide residue to full sequence) on the DNA template. Efficiency of the chemical ligation of RNA oligomers was shown to be much lower than that of DNA analogues. The coupling yield depends on the position of the RNA segment in the hybrid duplexes and on the position of the phosphate group in the nick.