David Garciadiego-Cázares - Academia.edu (original) (raw)

Papers by David Garciadiego-Cázares

Chondrogenic differentiation in the limb bud follows a proximal to distal sequence in developing ... more Chondrogenic differentiation in the limb bud follows a proximal to distal sequence in developing limb, resulting in cartilage elements only formed by proliferating chondrocytes. When the skeletal pattern is established the cells at specific regions of this cartilaginous primordium exit of the cell cycle, initiating joint formation, and delimiting the boundary of each individual skeletal element. Later in development, the cells in the centre of each skeletal element instead of initiating joint formation, they become pre-hyper-trophic, leading down the path to hypertrophy and eventual ossification. Although, Wnt14 is the first gene reported to have the ability to initiate joint formation, it is not known why proliferating chondrocytes become pre-joint cells or pre-hypertrophic cell during deve-lopment of appendicular skeleton. Recent evidence involves integrin signaling in the decision between two alternative fates by proliferating chondrocytes in the skeletal elements, pre-joint or p...

Histology and histopathology, 2015

Tissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting op... more Tissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting option for ear repair. Chitosan-Polyvinyl alcohol-Epichlorohydrine hydrogel (CS-PVA-ECH) is biocompatible and displays appropriate mechanical properties to be used as a scaffold. The present work, studies the potential of CS-PVA-ECH scaffolds seeded with chondrocytes to develop elastic cartilage engineered-neotissues. Chondrocytes isolated from rabbit and swine elastic cartilage were independently cultured onto CS-PVA-ECH scaffolds for 20 days to form the appropriate constructs. Then, in vitro cell viability and morphology were evaluated by calcein AM and EthD-1 assays and Scanning Electron Microscopy (SEM) respectively, and the constructs were implanted in nu/nu mice for four months, in order to evaluate the neotissue formation. Histological analysis of the formed neotissues was performed by Safranin O, Toluidine blue (GAG's), Verhoeff-Van Gieson (elastic fibers), Masson's trichro...

ASAIO Journal, 2015

Human adipose derived mesenchymal stem cells are thought to be potential key factors for starting... more Human adipose derived mesenchymal stem cells are thought to be potential key factors for starting the regenerative process after tissue injury. However, an efficient method of delivering these regenerative cells to an external wound site is still lacking. Human amnion and pig skin have long been used as skin wound dressings for the treatment of burns and other skin lesions. Here we present the generation of two constructs using these two biomaterials as effective scaffolds for the culture of human adipose derived mesenchymal stem cells(hADMSCs). It was found that hADMSCsseeded onto radio-sterilized human amnion (RHA) and pig skin (RPS) are viable and proliferate. These cells are able to migrate over these scaffoldsas demonstrated by using time-lapse microscopy. Additionally, the scaffolds induce hADMSCs to secrete interleukin-10, an important negative regulator of inflammation; and interleukin-1β, a pro-inflammatory protein.The interplay between these two proteins has been proven to be vital for a balanced restoration of all necessary tissues. Thus, radio-sterilized human amnion and pig skin are likely suitable scaffolds for delivery ofhuman adipose derived mesenchymal stem cellstransplants that could promote tissue regeneration in skin injuries like burned patients.

Annals of the New York Academy of Sciences

Cómo un grupo de células indiferenciadas pueden formar estructuras y tejidos altamente organizado... more Cómo un grupo de células indiferenciadas pueden formar estructuras y tejidos altamente organizados y diferenciados?, ¿Cuál es la naturaleza de las señales que dictan estos eventos? ¿Cómo son los procesos del desarrollo que actúan para que las diferentes especies desarrollen estructuras similares pero adaptadas al medio en el que viven? Uno de los modelos que han permitido responder en parte a estas interrogantes es la extremidad embrionaria. A partir de los estudios en la extremidad embrionaria se han propuesto una serie de interesantes paradigmas que han cambiado la percepción de ver a los procesos del desarrollo como aislados y poco dinámicos. En esta revisión, destacamos aquellos estudios que han contribuido de manera amplia a entender la inducción de la formación de la extremidad y el establecimiento de los centros organizadores que controlan su morfogénesis, así como el patrón esquelético que incluye la diferenciación del cartílago y la muerte celular. How can a group of undiff...

DESCELULARIZACIÓN DE ÓRGANOS Y TEJIDOS Autores: *David M. Giraldo1, Héctor Martinez1, Julieta Gar... more DESCELULARIZACIÓN DE ÓRGANOS Y TEJIDOS Autores: *David M. Giraldo1, Héctor Martinez1, Julieta García2, David Garciadiego2, Avelina Sotres3, Cristina Piña1 1Instituto de Investigaciones en Materiales, UNAM, Ciudad Universitaria, Circuito Exterior s/n, C.P. 04510, México D.F., México. 2Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación, Calzada México Xochimilco Nº 289, C.P. 14389, México D.F., México. 3Departamento de Investigación en Cirugía Experimental, Instituto Nacional de Enfermedades Respiratorias “Ismael Cossio Villegas”, Calzada de Tlalpan Nº 4502, C.P. 14080, México D.F., México. *Presentador Resumen: La descelularización es el proceso de remover los antígenos celulares alogénicos o xenogénicos del tejido los cuales podrían iniciar una respuesta inmune, mientras se deja una matriz extracelular (MEC) viable la cual se compone de una mezcla de moléculas estructurales y funcionales. Las estrategias de descelularización...

PLOS ONE, 2015

The Integrin β1 family is the major receptors of the Extracellular matrix (ECM), and the synthesi... more The Integrin β1 family is the major receptors of the Extracellular matrix (ECM), and the synthesis and degradation balance of ECM is seriously disrupted during Osteoarthritis (OA). In this scenario, integrins modify their pattern expression and regulate chondrocyte differentiation in the articular cartilage. Members of the Transforming growth factor beta (Tgf-β) Superfamily, such as Growth differentiation factor 5 (Gdf-5) and Bone morphogenetic protein 7 (Bmp-7), play a key role in joint formation and could regulate the integrin expression during chondrocyte differentiation and osteoarthritis progression in an experimental OA rat model. Decrease of α5 integrin expression in articular cartilage was related with chondrocyte dedifferentiation during OA progression, while increase of α1, α2, and α3 integrin expression was related with fibrous areas in articular cartilage during OA. Hypertrophic chondrocytes expressed αV integrin and was increased in the articular cartilage of rats with OA. Integrin expression during chondrocyte differentiation was also analyzed in a micromass culture system of mouse embryo mesenchymal cells, micromass cultures was treated with Gdf-5 or Bmp-7 for 4 and 6 days, respectively. Gdf-5 induced the expression of the α5 sub-unit, while Bmp-7 induced the expression of the αV sub-unit. This suggests a switch in signaling for prehypertrophic chondrocyte differentiation towards hypertrophy, where Gdf-5 could maintain the articular chondrocyte phenotype and Bmp-7 would induce hypertrophy. Decrease of Ihh expression during late stages of OA in rat model suggest that the ossification in OA rat knees and endochondral ossification could be activated by Bmp-7 and αV integrin in absence of Ihh. Thus, chondrocyte phenotype in articular cartilage is similar to prehypetrophic chondrocyte in growth plate, and is preserved due to the presence of Indian hedgehog (Ihh), Gdf-5 and α5 integrin to maintain articular cartilage and prevent hypertrophy.

Osteoarthritis and Cartilage, 2011

Reumatología Clínica, 2007

During skeletal development, the mechanisms of formation, differentiation and maturation of the c... more During skeletal development, the mechanisms of formation, differentiation and maturation of the cartilage are important steps in the skeleton morphogenesis; these mechanisms regulate growth of long bones and joint formation. Although cartilage in the growth plate and articular cartilage are very similar, they have some differences in the differentiation mechanisms. Growth plate cartilage regulates the growth of long bones by cartilage substitution of bone, while articular cartilage is maintained by delay of chondrocyte maturation and hypertrophy. Maybe, the mechanism of cartilage maturation is activated and increased during osteoarthritis, resulting in loss of the biomechanical properties required by the joints to resist the impact required by the skeleton to move.

Reumatología Clínica, 2007

a modern alternative to the treatment of these lesions and in this way, prevent the development o... more a modern alternative to the treatment of these lesions and in this way, prevent the development of early osteoarthritis in young active patients. Different tissue engineering approaches rely on the current use of autologous chondrocytes, or the potential use of mesenchymal stem cells. Other variables rely on the type of scaffold to use such as synthetic biodegradable polymers, fibrin or collagen-derived scaffolds of different sources, bovine, porcine, rat tail, etc, in the form of gels, sponges, mesh, etc, and all of these with or without growth factors. The use of autologous chondrocytes is a reality at the present time, whether injected under a periosteum patch or seeded on collagen. However, most investigators and biotech companies are in search of onestep surgical procedures, for which reason stem cells have to be kept in mind, as well as systems that will allow arthroscopic implantation.

MRS Proceedings, 2013

The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which ... more The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which can be used as xenograft for cartilage tissue regeneration.

Developmental Biology, 2007

Annals of the New York Academy of Sciences, 1998

Annals of the New York Academy of Sciences, 2007

Although the molecular mechanisms for initiation of cartilage destruction in osteoarthritis (OA) ... more Although the molecular mechanisms for initiation of cartilage destruction in osteoarthritis (OA) are unknown, it has been demonstrated that disruption of mitogen-inducible gene 6 (Mig-6) in mice leads to the onset of a degenerative joint disease like OA. On this basis, we correlated gene expression of Mig-6 with Wnt-9a and Wnt-7b genes; we showed downregulation of Mig-6, Wnt-7b, and Wnt-9a during OA, while Wnt-7b was expressed also in osteoblast-like cells. Here we suggest that Aggrecan degradation occurs before the downregulation of Mig-6. It remains to be proven whether there is any relation between Wnt signaling and Aggrecan degradation.

Chondrogenic differentiation in the limb bud follows a proximal to distal sequence in developing ... more Chondrogenic differentiation in the limb bud follows a proximal to distal sequence in developing limb, resulting in cartilage elements only formed by proliferating chondrocytes. When the skeletal pattern is established the cells at specific regions of this cartilaginous primordium exit of the cell cycle, initiating joint formation, and delimiting the boundary of each individual skeletal element. Later in development, the cells in the centre of each skeletal element instead of initiating joint formation, they become pre-hyper-trophic, leading down the path to hypertrophy and eventual ossification. Although, Wnt14 is the first gene reported to have the ability to initiate joint formation, it is not known why proliferating chondrocytes become pre-joint cells or pre-hypertrophic cell during deve-lopment of appendicular skeleton. Recent evidence involves integrin signaling in the decision between two alternative fates by proliferating chondrocytes in the skeletal elements, pre-joint or p...

Histology and histopathology, 2015

Tissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting op... more Tissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting option for ear repair. Chitosan-Polyvinyl alcohol-Epichlorohydrine hydrogel (CS-PVA-ECH) is biocompatible and displays appropriate mechanical properties to be used as a scaffold. The present work, studies the potential of CS-PVA-ECH scaffolds seeded with chondrocytes to develop elastic cartilage engineered-neotissues. Chondrocytes isolated from rabbit and swine elastic cartilage were independently cultured onto CS-PVA-ECH scaffolds for 20 days to form the appropriate constructs. Then, in vitro cell viability and morphology were evaluated by calcein AM and EthD-1 assays and Scanning Electron Microscopy (SEM) respectively, and the constructs were implanted in nu/nu mice for four months, in order to evaluate the neotissue formation. Histological analysis of the formed neotissues was performed by Safranin O, Toluidine blue (GAG's), Verhoeff-Van Gieson (elastic fibers), Masson's trichro...

ASAIO Journal, 2015

Human adipose derived mesenchymal stem cells are thought to be potential key factors for starting... more Human adipose derived mesenchymal stem cells are thought to be potential key factors for starting the regenerative process after tissue injury. However, an efficient method of delivering these regenerative cells to an external wound site is still lacking. Human amnion and pig skin have long been used as skin wound dressings for the treatment of burns and other skin lesions. Here we present the generation of two constructs using these two biomaterials as effective scaffolds for the culture of human adipose derived mesenchymal stem cells(hADMSCs). It was found that hADMSCsseeded onto radio-sterilized human amnion (RHA) and pig skin (RPS) are viable and proliferate. These cells are able to migrate over these scaffoldsas demonstrated by using time-lapse microscopy. Additionally, the scaffolds induce hADMSCs to secrete interleukin-10, an important negative regulator of inflammation; and interleukin-1β, a pro-inflammatory protein.The interplay between these two proteins has been proven to be vital for a balanced restoration of all necessary tissues. Thus, radio-sterilized human amnion and pig skin are likely suitable scaffolds for delivery ofhuman adipose derived mesenchymal stem cellstransplants that could promote tissue regeneration in skin injuries like burned patients.

Annals of the New York Academy of Sciences

Cómo un grupo de células indiferenciadas pueden formar estructuras y tejidos altamente organizado... more Cómo un grupo de células indiferenciadas pueden formar estructuras y tejidos altamente organizados y diferenciados?, ¿Cuál es la naturaleza de las señales que dictan estos eventos? ¿Cómo son los procesos del desarrollo que actúan para que las diferentes especies desarrollen estructuras similares pero adaptadas al medio en el que viven? Uno de los modelos que han permitido responder en parte a estas interrogantes es la extremidad embrionaria. A partir de los estudios en la extremidad embrionaria se han propuesto una serie de interesantes paradigmas que han cambiado la percepción de ver a los procesos del desarrollo como aislados y poco dinámicos. En esta revisión, destacamos aquellos estudios que han contribuido de manera amplia a entender la inducción de la formación de la extremidad y el establecimiento de los centros organizadores que controlan su morfogénesis, así como el patrón esquelético que incluye la diferenciación del cartílago y la muerte celular. How can a group of undiff...

DESCELULARIZACIÓN DE ÓRGANOS Y TEJIDOS Autores: *David M. Giraldo1, Héctor Martinez1, Julieta Gar... more DESCELULARIZACIÓN DE ÓRGANOS Y TEJIDOS Autores: *David M. Giraldo1, Héctor Martinez1, Julieta García2, David Garciadiego2, Avelina Sotres3, Cristina Piña1 1Instituto de Investigaciones en Materiales, UNAM, Ciudad Universitaria, Circuito Exterior s/n, C.P. 04510, México D.F., México. 2Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación, Calzada México Xochimilco Nº 289, C.P. 14389, México D.F., México. 3Departamento de Investigación en Cirugía Experimental, Instituto Nacional de Enfermedades Respiratorias “Ismael Cossio Villegas”, Calzada de Tlalpan Nº 4502, C.P. 14080, México D.F., México. *Presentador Resumen: La descelularización es el proceso de remover los antígenos celulares alogénicos o xenogénicos del tejido los cuales podrían iniciar una respuesta inmune, mientras se deja una matriz extracelular (MEC) viable la cual se compone de una mezcla de moléculas estructurales y funcionales. Las estrategias de descelularización...

PLOS ONE, 2015

The Integrin β1 family is the major receptors of the Extracellular matrix (ECM), and the synthesi... more The Integrin β1 family is the major receptors of the Extracellular matrix (ECM), and the synthesis and degradation balance of ECM is seriously disrupted during Osteoarthritis (OA). In this scenario, integrins modify their pattern expression and regulate chondrocyte differentiation in the articular cartilage. Members of the Transforming growth factor beta (Tgf-β) Superfamily, such as Growth differentiation factor 5 (Gdf-5) and Bone morphogenetic protein 7 (Bmp-7), play a key role in joint formation and could regulate the integrin expression during chondrocyte differentiation and osteoarthritis progression in an experimental OA rat model. Decrease of α5 integrin expression in articular cartilage was related with chondrocyte dedifferentiation during OA progression, while increase of α1, α2, and α3 integrin expression was related with fibrous areas in articular cartilage during OA. Hypertrophic chondrocytes expressed αV integrin and was increased in the articular cartilage of rats with OA. Integrin expression during chondrocyte differentiation was also analyzed in a micromass culture system of mouse embryo mesenchymal cells, micromass cultures was treated with Gdf-5 or Bmp-7 for 4 and 6 days, respectively. Gdf-5 induced the expression of the α5 sub-unit, while Bmp-7 induced the expression of the αV sub-unit. This suggests a switch in signaling for prehypertrophic chondrocyte differentiation towards hypertrophy, where Gdf-5 could maintain the articular chondrocyte phenotype and Bmp-7 would induce hypertrophy. Decrease of Ihh expression during late stages of OA in rat model suggest that the ossification in OA rat knees and endochondral ossification could be activated by Bmp-7 and αV integrin in absence of Ihh. Thus, chondrocyte phenotype in articular cartilage is similar to prehypetrophic chondrocyte in growth plate, and is preserved due to the presence of Indian hedgehog (Ihh), Gdf-5 and α5 integrin to maintain articular cartilage and prevent hypertrophy.

Osteoarthritis and Cartilage, 2011

Reumatología Clínica, 2007

During skeletal development, the mechanisms of formation, differentiation and maturation of the c... more During skeletal development, the mechanisms of formation, differentiation and maturation of the cartilage are important steps in the skeleton morphogenesis; these mechanisms regulate growth of long bones and joint formation. Although cartilage in the growth plate and articular cartilage are very similar, they have some differences in the differentiation mechanisms. Growth plate cartilage regulates the growth of long bones by cartilage substitution of bone, while articular cartilage is maintained by delay of chondrocyte maturation and hypertrophy. Maybe, the mechanism of cartilage maturation is activated and increased during osteoarthritis, resulting in loss of the biomechanical properties required by the joints to resist the impact required by the skeleton to move.

Reumatología Clínica, 2007

a modern alternative to the treatment of these lesions and in this way, prevent the development o... more a modern alternative to the treatment of these lesions and in this way, prevent the development of early osteoarthritis in young active patients. Different tissue engineering approaches rely on the current use of autologous chondrocytes, or the potential use of mesenchymal stem cells. Other variables rely on the type of scaffold to use such as synthetic biodegradable polymers, fibrin or collagen-derived scaffolds of different sources, bovine, porcine, rat tail, etc, in the form of gels, sponges, mesh, etc, and all of these with or without growth factors. The use of autologous chondrocytes is a reality at the present time, whether injected under a periosteum patch or seeded on collagen. However, most investigators and biotech companies are in search of onestep surgical procedures, for which reason stem cells have to be kept in mind, as well as systems that will allow arthroscopic implantation.

MRS Proceedings, 2013

The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which ... more The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which can be used as xenograft for cartilage tissue regeneration.

Developmental Biology, 2007

Annals of the New York Academy of Sciences, 1998

Annals of the New York Academy of Sciences, 2007

Although the molecular mechanisms for initiation of cartilage destruction in osteoarthritis (OA) ... more Although the molecular mechanisms for initiation of cartilage destruction in osteoarthritis (OA) are unknown, it has been demonstrated that disruption of mitogen-inducible gene 6 (Mig-6) in mice leads to the onset of a degenerative joint disease like OA. On this basis, we correlated gene expression of Mig-6 with Wnt-9a and Wnt-7b genes; we showed downregulation of Mig-6, Wnt-7b, and Wnt-9a during OA, while Wnt-7b was expressed also in osteoblast-like cells. Here we suggest that Aggrecan degradation occurs before the downregulation of Mig-6. It remains to be proven whether there is any relation between Wnt signaling and Aggrecan degradation.