Dewi Sukmawati - Academia.edu (original) (raw)

Papers by Dewi Sukmawati

The Indonesian Biomedical Journal

BACKGROUND: Nephrotic syndrome (NS) is the most common glomerular disease in childhood. The propo... more BACKGROUND: Nephrotic syndrome (NS) is the most common glomerular disease in childhood. The proposed hypothesis for the pathogenesis of this disease has changed over time, from immune dysregulation theory and systemic circulating factors theory, to the growing recognition of podocytopathies’ role. The existance of podocytopathies is usually examined by using podocyte-derived microparticles (MPs), such as nephrin, podocin, and podocalyxin (PCX). Therefore in this study, the difference between nephrin, podocin, and PCX expressions in NS children and healthy children was investigated.METHODS: An observational cross-sectional study was conducted, involving 33 children with NS and 22 age-matched healthy children as controls. Urine samples were collected from each subject in the early morning, before being processed and incubated with antibodies to detect nephrin, podocin, and PCX. The processed samples were then analyzed with flow cytometer methods.RESULTS: NS subjects had significantly ...

Journal of applied pharmaceutical science, 2024

Journal of Diabetes and Its Complications, 2016

Aims: To investigate the role of Notch signaling pathway in vasculogenic dysfunction of diabetic ... more Aims: To investigate the role of Notch signaling pathway in vasculogenic dysfunction of diabetic EPCs (DM-EPCs). Methods: the study was performed in mice and that diabetes was induced with Streptozotocin. The functional consequences of Notch pathway modulation were studied by assessment of colony forming capacity (EPC colony forming assay), EPC differentiation capacity (% of definitive EPC-CFU (dEPC-CFU)) , circulating EPCs (EPC culture assay) and migrated cells (migration assay); in the presence of Notch inhibitor (-secretase inhibitors (GSI)) compared to control. Notch pathway and VEGF involvement in DM-EPCs were assessed by gene expression (RT-qPCR). Results: DM demonstrated to increase Notch pathway expression in bone marrow (BM) EPCs followed by lower in EPC-CFU number, EPCs differentiation capacity, number of circulating EPCs, migrated cells and VEGF expression compared to control (p<0.05). Inhibition of Notch pathway by GSI rescued vasculogenic dysfunction in DM-EPCs as represented by increased in EPC-CFU number, differentiation capacity and number of circulating EPCs (p<0.05). Conclusion: Our findings indicate the involvement of Notch pathway in mediating DM-EPCs dysfunction including less number of EPC-CFU, circulating EPCs and migrated cell number compared to control. Further in vitro inhibition of Notch pathway by GSI rescued DM-EPCs dysfunction. Therefore targeting Notch pathway in DM may provide a target to restore DM-EPC dysfunction.

Journal of Global Pharma Technology, Jul 7, 2017

Abstract : Objective: to analyzevarious growth factor (GF) content in umbilical cord derived mese... more Abstract : Objective: to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell ( UC- MSC) culture waste at harvest. Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests. We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium waste s using ELISA method. We grouped GF concentrations according to various culture length sat harvest , and calculated them ean values and standard deviations of the various GF . We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration . Results: EGF , VEGF and PlGF concentrations were highest at harvest day-3, while NGF concentration was the highest at harvest-day-5 , respectively . Therefore, overall highest concentration of most GF was at harvest day 3. Conclusion: UC- MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF , which might be used in regenerative medicine.

PubMed, 2015

The concept of Endothelial Progenitor Cells (EPCs) therapy for adult neovascularization has conti... more The concept of Endothelial Progenitor Cells (EPCs) therapy for adult neovascularization has continuously received attention. They are believed to participate in endothelial repair and post natal angiogenesis due to their abilities in differentiating into endothelial cells and producing protective cytokines and growth factors. Abundant evidence supports the involvement of EPCs in capillary growth and in participating in the formation of collateral vessels, which lead to improved vascular perfusion and functional recovery in target tissue. Autologous EPC now is becoming a novel treatment option for therapeutic revascularization and vascular repair in ischemic diseases. However, various diseases such as diabetes, heart disease and ischemic diseases are related to EPC dysfunction and give rise to additional challenges of autologous EPC therapy. A novel strategy to enhance the number and function of EPCs is needed to be established to provide successful autologous EPCs therapy. Currently, clinical trials for the new generation of EPC therapy in treating peripheral ischemic diseases are underway. In this review we provide an overview and the limitations of current EPCs therapy with an introduction to the new strategies of next generation EPC therapy for more promising vascular and tissue regeneration therapy.

FABAD journal of pharmaceutical sciences, 2021

Endocrinology, Mar 1, 2013

Dipeptyl peptidase-4 (DPP-4) inhibitors modulate the progression of atherosclerosis. To gain insi... more Dipeptyl peptidase-4 (DPP-4) inhibitors modulate the progression of atherosclerosis. To gain insights into their mechanism of action, 9-wk-old male apolipoprotein E (apoE)-deficient mice were fed a DPP-4 inhibitor, anagliptin-containing diet. The effects of anagliptin were investigated in, a monocyte cell line, human THP-1 cells, and rat smooth muscle cells (SMCs). Treatment with anagliptin for 16 wk significantly reduced accumulation of monocytes and macrophages in the vascular wall, SMC content in plaque areas, and oil red O-stained area around the aortic valve without affecting glucose tolerance or body weight. Serum DPP-4 concentrations were significantly higher in apoE-deficient mice than control mice, and the levels increased with aging, suggesting the involvement of DPP-4 in the progression of atherosclerosis. Indeed, soluble DPP-4 augmented cultured SMC proliferation, and anagliptin suppressed the proliferation by inhibiting ERK phosphorylation. In THP-1 cells, anagliptin reduced lipopolysaccharide-induced TNF-␣ production with inhibiting ERK phosphorylation and nuclear translocation of nuclear factor-B. Quantitative analysis also showed that anagliptin reduced the area of atherosclerotic lesion in apoE-deficient mice. These results indicated that the anti-atherosclerotic effect of anagliptin is mediated, at least in part, through its direct inhibition of SMC proliferation and inflammatory reaction of monocytes. (Endocrinology 154: 1260-1270, 2013) G lucagon-like peptide-1 (GLP-1) is a hormone secreted from the L cells of the small intestine and stimulates glucose-dependent insulin response (1-3). In addition, GLP-1 has other effects that may improve the pathophysiology of diabetic state, such as suppression of glucagon secretion (2), inhibition of gastrointestinal secretion and motility (4), and inhibition of food intake (5). In diabetic rodents, enhancement of GLP-1 action increases pancreatic ␤-cell mass (6-8). Accordingly, enhancement of GLP-1 action appears to have ideal profiles for the treatment of type 2 diabetes mellitus. Dipeptidyl peptidase-4 (DPP-4) (also known as CD26) is a membrane-bound exopeptidase that rapidly degrades GLP-1. Thus, DPP-4 inhibitors are theoretically useful for the treatment for type 2 diabetes by raising the concentration of GLP-1.

Advanced Science Letters, Aug 1, 2018

The transmembrane ligand ephrin-B2 is molecular marker for arterial endothelial cells and early b... more The transmembrane ligand ephrin-B2 is molecular marker for arterial endothelial cells and early blood vessel formation. Neovascularization is one of the important event that support wound healing. Adiposederived stem cells conditioned medium (ADSC-CM) is known to contain cytokines and growth factors as regenerative agent. This study aims to investigate the effect of ADSC-CM on ephrin-B2 expression that represent as neovascularization marker in burn wound granulation tissue. Male Sprague dawley rats were randomly divided into control, conditioned medium (CM) and medium complete (MC) groups. Full-thickness burn wounds were created by placing preheated metal plat on dorsal thorax of the prepared rats. The burn wounds were treated once daily according to their group. Skin wound tissues were collected at day 7, 14, and 21 post wounded for histological analysis. The expression of ephrin-B2 was demonstrated by immunohistochemistry staining. Neovascularization evaluation was done by calculating the number of newly formed capillaries that expressed Ephrin-B2 in wound granulation tissues. Expression of Ephrin-B2 in burn wound tissue was prominently increased in CM compared to control and MC groups on day 7, 14 and 21 post wounded. The CM group also showed higher neovascular number in granulation tissue compared to control and MC group on day 7, 14 and 21 (p &lt; 0.05). Our results showed prominent ephrin-B2 expression in burn wound tissue after treated with ADSC-CM. Topical application of ADSC-CM has shown to promote angiogenesis in full-thickness burn wound in part through the increased of ephrin-B2 expression. Therefore ADSC-CM could act as an alternative strategy to promote burn wound healing.

Medical Journal of Indonesia, May 1, 2009

Tujuan Penelitian ini dilakukan untuk melihat asosiasi antara kehadiran dalam kuliah dan nilai uj... more Tujuan Penelitian ini dilakukan untuk melihat asosiasi antara kehadiran dalam kuliah dan nilai ujian.

Indonesian Journal of Obstetrics and Gynecology

and its correlation to i mRNA expression in low-grade and high-grade EOC’s patients from Dr. Cipt... more and its correlation to i mRNA expression in low-grade and high-grade EOC’s patients from Dr. Cipto Mangunkusumo General hospital, Indonesia. Methods: This study is cross-sectional with a total of 65 healthy subjects and 80 ovarian biopsies (15 ovarian cysts as expression calibrators, 36 low-grade EOC, and 29 high-grade EOC) were used in this study. The distribution of genotypes and alleles was analyzed using ARMS PCR. The mRNA expressions of RELA were determined by real-time polymerase chain reaction (qPCR) analysis.Results: There was no significant difference between genotype and allele distributions for RELA rs11820062 in normal and case group. RELA relative mRNA expression was significantly higher in low-grade and high-grade EOC compared to in ovarian cysts (p<0.01). RELA rs11820062 CC genotype correlated to higher RELA mRNA relative expression and the TT genotype of RELA rs11820062 correlated with lower RELA mRNA relative expression in low-grade and high-grade EOC.Conclusion:...

Medical Education Unit FKUI, 2019

Journal of Applied Pharmaceutical Science

The present study aimed to investigate whether curcumin in nanoparticle form may provide hepatopr... more The present study aimed to investigate whether curcumin in nanoparticle form may provide hepatoprotection in an ovarian cancer model in rats treated with cisplatin. Twenty-five female Wistar rats were divided into five 4-week treatment groups: (1) healthy rat group and four groups of 7,12-dimethylbenz(a)anthracene-induced ovarian cancer model rats receiving (2) vehicle only, (3) cisplatin 4 mg/kg BW/week, (4) cisplatin 4 mg/kg BW/week + curcumin 100 mg/kg BW/day, and (5) cisplatin 4 mg/kg BW/week + nanocurcumin (NC) 100 mg/kg BW/day. At the end of the treatment, a histopathological evaluation was carried out on the liver samples, in addition to oxidative stress, apoptosis, inflammatory, and fibrosis markers analyses. The group treated with cisplatin + NC had lower aspartate transaminase and alanine transaminase levels and lowered malondialdehyde and higher glutathione levels than those treated with cisplatin only. In addition, the nuclear factor-erythroid factor 2-related factor 2/Kelch-like ECH-associated protein 1 pathway genes were upregulated in the group treated with cisplatin + NC. We also demonstrated that NC effectively suppressed the gene expression of inflammatory marker tumor necrosis factor-α, as well as fibrosis marker transforming growth factor-β1. Furthermore, NC treatment reduced the fibrotic area in the hepatic tissues, as shown by Masson's trichrome staining. In conclusion, the hepatoprotective effects of NC are mediated through the modulation of antioxidative and anti-inflammatory pathways.

Fakultas Kedokteran UI, 2018

Medical Education Unit Fakultas Kedokteran Universitas Indonesia, 2019

Circulation, 2016

Introduction: Endothelial progenitor cells (EPCs) in murine bone marrow lineage-negative c-kit+Sc... more Introduction: Endothelial progenitor cells (EPCs) in murine bone marrow lineage-negative c-kit+Sca-1+ (BM-KSL) fraction are mobilized in circulation upon tissue injury to mediate a neovascularization process called vasculogenesis, which requires proper differentiative capacity of EPCs. Diabetics suffer defective vasculogenesis, a substantial mechanism for diabetic angiopathy in ischemic limb and heart. How it occurs, however, remains largely unknown. We recently demonstrated that type 1 (T1) and T2 diabetes (DM) induces a transcriptional coactivator PGC-1α in endothelial cells (ECs), and that EC PGC-1α mediates impaired cell migration and angiogenesis in DM. PGC-1α was also found to increase in EPCs cultivated from T2DM patients PB-MNCs. Building on these, we hypothesized roles for PGC-1α in EPC dysfunction. Methods and Results: We used Tie2-Cre-driven PGC-1α conditional null mice (KO), with or without T1DM induction by streptozocine. DM increased PGC-1α mRNA in WT BM-KSL cells wher...

Plastic and Reconstructive Surgery, 2012

International Journal of Applied Pharmaceutics

Objective: We aimed to predict the PPI network and in silico analysis of a drug that can potentia... more Objective: We aimed to predict the PPI network and in silico analysis of a drug that can potentially inhibit colon cancer, specifically in the Wnt/β-catenin signaling pathway, based on pharmacophore modeling and molecular docking. Methods: Target genes involved in colon development were screened for specific genes in the Wnt/b-catenin signaling pathway. Tissue construction and possible signaling pathways were analyzed using protein-protein interactions. Genes with significant centrality and best-grade values were made to feature pharmacophore models and their suitability for potential drugs. Validation was carried out using the molecular docking method for interaction with the best Hits. Results: Protein-Protein Interaction Network (PPI) revealed BTNNB1, TP53, AXIN, FZD-8, and CDK1 as potential critical targets in the Wnt/β-catenin signaling pathway and from the suitability of pharmacophore features obtained 27 drugs as the best Hit compounds. The therapeutic effects of the drugs ...