Lawrence Diamond - Academia.edu (original) (raw)

Papers by Lawrence Diamond

American journal of clinical pathology, 1992

The histopathologic features of 251 lymph nodes obtained from 200 patients with various clinical ... more The histopathologic features of 251 lymph nodes obtained from 200 patients with various clinical expressions of cutaneous T-cell lymphoma (mycosis fungoides and Sezary syndrome) were reviewed retrospectively. Lymphomatous involvement, defined as partial or complete effacement of lymph node architecture by malignant cells, was identified in 89 specimens (35%) and was characterized by morphologic variability from case to case. The involved specimens were classified into four major histologic subtypes according to the morphologic appearance of the malignant cells in a manner analogous to a modified Rappaport classification of diffuse non-Hodgkin's lymphomas. Although lymph node involvement was associated with a poor prognosis regardless of histologic subtype, the survival of patients with small cell (cerebriform) subtype was found to be significantly better (median survival time, 40 months) than other subtypes (median survival time, 20 months), possibly because this type of involve...

Computer Methods and Programs in Biomedicine, 1995

Knowledge-based systems in diagnostic medicine are often used for teaching medical decision makin... more Knowledge-based systems in diagnostic medicine are often used for teaching medical decision making. We have extended the educational value of our suite of hematology decision support systems (peripheral blood analysis, flow cytometry immunophenotyping and DNA analysis, and bone marrow morphology) by creating case studies in a hypertext format. The case studies use the knowledge-based system screens as a background. Digitized images of blood and bone marrow smears from the teaching cases can be accessed on-line. The case studies, which are designed primarily for technologists and physicians, emphasize the proper multiparameter approach to hematopathology diagnosis.

European Journal of Haematology, 2009

Abstract: This study focused on the efficacy of IDEC-C2B8 (chimeric anti-CD20) immunotherapy rel... more Abstract: This study focused on the efficacy of IDEC-C2B8 (chimeric anti-CD20) immunotherapy relative to specific subtypes of low-grade lymphoproliferative disorders/non-Hodgkin's lymphomas (LPD/NHL). Forty-eight patients with resistant or relapsed disease completed the IDEC-C2B8 infusion schedule of 375 mg/m2/wk × 4 wk. The LPD/NHL subtypes included: (a) follicular centre cell lymphoma (FCC) in 22 patients; (b) mantle cell lymphoma (MCL) in 10; (c) 1 diffuse large cell lymphoma (DLCL); and (d) the category of small lymphocytic lymphoma/chronic lymphocytic leukaemia (SLL/CLL) and related disorders in 15 patients. No patient obtained a complete remission. Ten patients (21%) achieved partial remission: 6 FCC, 2 MCL, 1 DLCL and 1 patient from the SLL/CLL group. Twenty-eight patients had stable disease and 10 progressed during immunotherapy. In patients with CLL and MCL in leukaemic phase, there was no correlation between the marked decrease in circulating neoplastic cells followin...

American Journal of Clinical Pathology, 1981

A formal evaluation was performed between two knowledge-based systems for lymph node pathology: 1... more A formal evaluation was performed between two knowledge-based systems for lymph node pathology: 1) Intellipath, Kiel edition, a Bayesian system; and, 2) “Professor Amadeus”, which uses categorical reasoning and defined diagnostic patterns. The evaluation, involving three pathologists, was based on 57 lymph node biopsies. Intellipath demonstrated satisfactory performance, with from 63.2% to 71.9% correct answers, depending on the experience level of the pathologist. “Professor Amadeus” achieved better results, with accuracy rates ranging from 93% to 96.5%. In this study, the better performance of “Professor Amadeus” could be attributed to a more effective multiparameter approach, fewer errors in the knowledge base, and better handling of input parameters.

A medical knowledge-based system for hemogram and peripheral blood smear interpretation was teste... more A medical knowledge-based system for hemogram and peripheral blood smear interpretation was tested in three situations outside of the developers' laboratory: Coulter Corporation's Clinical Evaluations, and two clinical laboratories in the United States and Canada. Rigorous testing of the system's functionality was performed. Hazard testing caught several programming errors which were corrected. The outside evaluators requested a number of changes to the screens and additional functionality. As a result, several user configurable features were added to the system. Medical information collected from 13 of 392 (3.3%) cases effected the knowledge base. Only two of the evaluator's comments concerned the style of the system's displayed output. The system was able to maintain high quality interpretive reports, appropriate to patient care, despite the expected differences in subjective morphologic interpretation between observers.

Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by ... more Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by serum, but had no effect on the accumulation of cellular ribonucleic acid. Simian virus 40 infection or manual microinjection of cloned fragments from the simian virus 40 A gene caused quiescent 3T3 cells to enter the S phase even in the presence of butyrate. NGI cells, a line of 3T3 cells transformed by simian virus 40, grew vigorously in 3 mM butyrate. Homokaryons were formed between G1 and S-phase 3T3 cells. Butyrate inhibited the induction of deoxyribonucleic acid synthesis that usually occurs in G1 nuclei when G1 cells are fused with S-phase cells. However, when G1 3T3 cells were fused with exponentially growing NGI cells, the 3T3 nuclei were induced to enter deoxyribonucleic acid synthesis. In tsAF8 cells, a ribonucleic acid polymerase II mutant that stops in the G1 phase of the cell cycle, no temporal sequence was demonstrated between the butyrate block and the temperature-sensitive block. These results confirm previous reports that certain virally coded proteins can induce cell deoxyribonucleic acid synthesis in the absence of cellular functions that are required by serumstimulated cells. Our interpretation of these data is that butyrate inhibited cell 1038 on July 14, 2016 by guest

Journal of Investigative Dermatology, Apr 1, 1988

Proceedings of the Annual Symposium on Computer Application in Medical Care, 1993

Cancer, 1994

in patients with uneffaced LN when classified according to the NCI-VA (LNO-2 versus LN3) or Dutch... more in patients with uneffaced LN when classified according to the NCI-VA (LNO-2 versus LN3) or Dutch (GrO-1 versus Gr2) ratings. In addition, all classification systems demonstrated a poor survival time associated with effaced LN. By combining results from the modified Rappaport and Dutch classifications, three prognostic groups could be identified based on cell morphology: a low-grade category with a small cell histologic subtype (median survival time, 40 months); a high-grade immunoblastic subtype (median survival time, 9 months) composed of cells with an oval nucleus containing a large, usually solitary central nucleolus; and an intermediate-grade category composed of all cases without the distinctive small cell and immunoblastic morphologies (median survival time, 26 months).

Cancer Research, Mar 1, 1980

4 The abbreviations used are: FMF, flow microfluorometry; PHA, phytohemag glutinin; MV, modal vol... more 4 The abbreviations used are: FMF, flow microfluorometry; PHA, phytohemag glutinin; MV, modal volume; CEN. chicken erythrocyte nuclei; cv, coefficient of variation.

Cancer, Aug 1, 1979

T-and B-cell markers were studied on suspensions of lymphoid cells obtained from two Warthin's tu... more T-and B-cell markers were studied on suspensions of lymphoid cells obtained from two Warthin's tumors. Each tumor was composed predominantly of Tlymphocytes and a smaller percentage of B-lymphocytes with a polyclonal distribution of their surface immunoglobulin. These results do not differ from those obtained in normal or reactive lymph nodes. Together with the finding of normal lymph node structures in these tumors, our findings support the concept that the lymphoid component of Warthin's tumor represents preexisting lymph node tissue.

Int J Bio Med Comput, 1994

Accurate diagnosis and classification of hematologic malignancies (acute leukemias and chronic ly... more Accurate diagnosis and classification of hematologic malignancies (acute leukemias and chronic lymphoproliferative disorders) requires a multiparameter approach including peripheral blood analysis, bone marrow examination, and immunophenotyping. We have designed knowledge-based computer systems for interpretation of the hemogram and peripheral blood smears, analysis of flow cytometric immunophenotyping panels, and morphologic assessment of bone marrow specimens. The 3 modules share a relational database which includes pertinent clinical history in addition to the laboratory results. The bone marrow module automatically writes a complete interpretative report with a final diagnosis by searching all of the databases for appropriate clinical, peripheral blood, and immunophenotyping information. The ability of the 3 modules to interact, and the quality of the interpretative reports were tested on 100 consecutive patients with leukemia. The final diagnosis made by the bone marrow system agreed with the hospital diagnosis in 94 cases and the authors' interpretation in 99 of 100 cases.

Flow Cytometry in Hematopathology, 2003

Cytometry, 1994

... Lawrence W. Diamond,' Doyen T. Nguyen, Michael Andreeff, Russell L. Maiese, and Raul C. B... more ... Lawrence W. Diamond,' Doyen T. Nguyen, Michael Andreeff, Russell L. Maiese, and Raul C. Braylan Department of Pathology, University of Cologne, Cologne, Germany ... At least two points must be scored for each of two different lineages to consider a leuke-mia biphenotypic. ...

Flow Cytometry in Hematopathology, 2003

Flow Cytometry in Hematopathology, 2003

Flow Cytometry in Hematopathology, 2003

Cytometry, 1980

The percentage of cells with S phase DNA content (S cells) was determined by flow cytometry in ce... more The percentage of cells with S phase DNA content (S cells) was determined by flow cytometry in cell suspensions obtained from 27 patients with non-Hodgkin's lymphomas. DNA was measured in ethanol-fixed cells stained with propidium iodide after RNase treatment. Cells from 17 samples were also exposed to [3H]-thymidine and labeling indices were determined. An excellent linear correlation was observed between the percentage of S cells calculated by flow cytometry and the labeling index (r = 0.95). To determine the relationship between the percentage of S cells and the clinical behavior of the tumors, the survival of the 27 patients was analyzed. None of the patients was undergoing antineoplastic therapy at the time of the study. The patients were separated into 2 groups according to the percentage of S cells calculated by flow cytometry: those with less than 5% S cells and those with higher than 5% S cells. Clinical follow-up ranged from 5 to 20 months. While 7 of 12 patients with ...

American journal of clinical pathology, 1992

The histopathologic features of 251 lymph nodes obtained from 200 patients with various clinical ... more The histopathologic features of 251 lymph nodes obtained from 200 patients with various clinical expressions of cutaneous T-cell lymphoma (mycosis fungoides and Sezary syndrome) were reviewed retrospectively. Lymphomatous involvement, defined as partial or complete effacement of lymph node architecture by malignant cells, was identified in 89 specimens (35%) and was characterized by morphologic variability from case to case. The involved specimens were classified into four major histologic subtypes according to the morphologic appearance of the malignant cells in a manner analogous to a modified Rappaport classification of diffuse non-Hodgkin's lymphomas. Although lymph node involvement was associated with a poor prognosis regardless of histologic subtype, the survival of patients with small cell (cerebriform) subtype was found to be significantly better (median survival time, 40 months) than other subtypes (median survival time, 20 months), possibly because this type of involve...

Computer Methods and Programs in Biomedicine, 1995

Knowledge-based systems in diagnostic medicine are often used for teaching medical decision makin... more Knowledge-based systems in diagnostic medicine are often used for teaching medical decision making. We have extended the educational value of our suite of hematology decision support systems (peripheral blood analysis, flow cytometry immunophenotyping and DNA analysis, and bone marrow morphology) by creating case studies in a hypertext format. The case studies use the knowledge-based system screens as a background. Digitized images of blood and bone marrow smears from the teaching cases can be accessed on-line. The case studies, which are designed primarily for technologists and physicians, emphasize the proper multiparameter approach to hematopathology diagnosis.

European Journal of Haematology, 2009

Abstract: This study focused on the efficacy of IDEC-C2B8 (chimeric anti-CD20) immunotherapy rel... more Abstract: This study focused on the efficacy of IDEC-C2B8 (chimeric anti-CD20) immunotherapy relative to specific subtypes of low-grade lymphoproliferative disorders/non-Hodgkin's lymphomas (LPD/NHL). Forty-eight patients with resistant or relapsed disease completed the IDEC-C2B8 infusion schedule of 375 mg/m2/wk × 4 wk. The LPD/NHL subtypes included: (a) follicular centre cell lymphoma (FCC) in 22 patients; (b) mantle cell lymphoma (MCL) in 10; (c) 1 diffuse large cell lymphoma (DLCL); and (d) the category of small lymphocytic lymphoma/chronic lymphocytic leukaemia (SLL/CLL) and related disorders in 15 patients. No patient obtained a complete remission. Ten patients (21%) achieved partial remission: 6 FCC, 2 MCL, 1 DLCL and 1 patient from the SLL/CLL group. Twenty-eight patients had stable disease and 10 progressed during immunotherapy. In patients with CLL and MCL in leukaemic phase, there was no correlation between the marked decrease in circulating neoplastic cells followin...

American Journal of Clinical Pathology, 1981

A formal evaluation was performed between two knowledge-based systems for lymph node pathology: 1... more A formal evaluation was performed between two knowledge-based systems for lymph node pathology: 1) Intellipath, Kiel edition, a Bayesian system; and, 2) “Professor Amadeus”, which uses categorical reasoning and defined diagnostic patterns. The evaluation, involving three pathologists, was based on 57 lymph node biopsies. Intellipath demonstrated satisfactory performance, with from 63.2% to 71.9% correct answers, depending on the experience level of the pathologist. “Professor Amadeus” achieved better results, with accuracy rates ranging from 93% to 96.5%. In this study, the better performance of “Professor Amadeus” could be attributed to a more effective multiparameter approach, fewer errors in the knowledge base, and better handling of input parameters.

A medical knowledge-based system for hemogram and peripheral blood smear interpretation was teste... more A medical knowledge-based system for hemogram and peripheral blood smear interpretation was tested in three situations outside of the developers' laboratory: Coulter Corporation's Clinical Evaluations, and two clinical laboratories in the United States and Canada. Rigorous testing of the system's functionality was performed. Hazard testing caught several programming errors which were corrected. The outside evaluators requested a number of changes to the screens and additional functionality. As a result, several user configurable features were added to the system. Medical information collected from 13 of 392 (3.3%) cases effected the knowledge base. Only two of the evaluator's comments concerned the style of the system's displayed output. The system was able to maintain high quality interpretive reports, appropriate to patient care, despite the expected differences in subjective morphologic interpretation between observers.

Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by ... more Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by serum, but had no effect on the accumulation of cellular ribonucleic acid. Simian virus 40 infection or manual microinjection of cloned fragments from the simian virus 40 A gene caused quiescent 3T3 cells to enter the S phase even in the presence of butyrate. NGI cells, a line of 3T3 cells transformed by simian virus 40, grew vigorously in 3 mM butyrate. Homokaryons were formed between G1 and S-phase 3T3 cells. Butyrate inhibited the induction of deoxyribonucleic acid synthesis that usually occurs in G1 nuclei when G1 cells are fused with S-phase cells. However, when G1 3T3 cells were fused with exponentially growing NGI cells, the 3T3 nuclei were induced to enter deoxyribonucleic acid synthesis. In tsAF8 cells, a ribonucleic acid polymerase II mutant that stops in the G1 phase of the cell cycle, no temporal sequence was demonstrated between the butyrate block and the temperature-sensitive block. These results confirm previous reports that certain virally coded proteins can induce cell deoxyribonucleic acid synthesis in the absence of cellular functions that are required by serumstimulated cells. Our interpretation of these data is that butyrate inhibited cell 1038 on July 14, 2016 by guest

Journal of Investigative Dermatology, Apr 1, 1988

Proceedings of the Annual Symposium on Computer Application in Medical Care, 1993

Cancer, 1994

in patients with uneffaced LN when classified according to the NCI-VA (LNO-2 versus LN3) or Dutch... more in patients with uneffaced LN when classified according to the NCI-VA (LNO-2 versus LN3) or Dutch (GrO-1 versus Gr2) ratings. In addition, all classification systems demonstrated a poor survival time associated with effaced LN. By combining results from the modified Rappaport and Dutch classifications, three prognostic groups could be identified based on cell morphology: a low-grade category with a small cell histologic subtype (median survival time, 40 months); a high-grade immunoblastic subtype (median survival time, 9 months) composed of cells with an oval nucleus containing a large, usually solitary central nucleolus; and an intermediate-grade category composed of all cases without the distinctive small cell and immunoblastic morphologies (median survival time, 26 months).

Cancer Research, Mar 1, 1980

4 The abbreviations used are: FMF, flow microfluorometry; PHA, phytohemag glutinin; MV, modal vol... more 4 The abbreviations used are: FMF, flow microfluorometry; PHA, phytohemag glutinin; MV, modal volume; CEN. chicken erythrocyte nuclei; cv, coefficient of variation.

Cancer, Aug 1, 1979

T-and B-cell markers were studied on suspensions of lymphoid cells obtained from two Warthin's tu... more T-and B-cell markers were studied on suspensions of lymphoid cells obtained from two Warthin's tumors. Each tumor was composed predominantly of Tlymphocytes and a smaller percentage of B-lymphocytes with a polyclonal distribution of their surface immunoglobulin. These results do not differ from those obtained in normal or reactive lymph nodes. Together with the finding of normal lymph node structures in these tumors, our findings support the concept that the lymphoid component of Warthin's tumor represents preexisting lymph node tissue.

Int J Bio Med Comput, 1994

Accurate diagnosis and classification of hematologic malignancies (acute leukemias and chronic ly... more Accurate diagnosis and classification of hematologic malignancies (acute leukemias and chronic lymphoproliferative disorders) requires a multiparameter approach including peripheral blood analysis, bone marrow examination, and immunophenotyping. We have designed knowledge-based computer systems for interpretation of the hemogram and peripheral blood smears, analysis of flow cytometric immunophenotyping panels, and morphologic assessment of bone marrow specimens. The 3 modules share a relational database which includes pertinent clinical history in addition to the laboratory results. The bone marrow module automatically writes a complete interpretative report with a final diagnosis by searching all of the databases for appropriate clinical, peripheral blood, and immunophenotyping information. The ability of the 3 modules to interact, and the quality of the interpretative reports were tested on 100 consecutive patients with leukemia. The final diagnosis made by the bone marrow system agreed with the hospital diagnosis in 94 cases and the authors' interpretation in 99 of 100 cases.

Flow Cytometry in Hematopathology, 2003

Cytometry, 1994

... Lawrence W. Diamond,' Doyen T. Nguyen, Michael Andreeff, Russell L. Maiese, and Raul C. B... more ... Lawrence W. Diamond,' Doyen T. Nguyen, Michael Andreeff, Russell L. Maiese, and Raul C. Braylan Department of Pathology, University of Cologne, Cologne, Germany ... At least two points must be scored for each of two different lineages to consider a leuke-mia biphenotypic. ...

Flow Cytometry in Hematopathology, 2003

Flow Cytometry in Hematopathology, 2003

Flow Cytometry in Hematopathology, 2003

Cytometry, 1980

The percentage of cells with S phase DNA content (S cells) was determined by flow cytometry in ce... more The percentage of cells with S phase DNA content (S cells) was determined by flow cytometry in cell suspensions obtained from 27 patients with non-Hodgkin's lymphomas. DNA was measured in ethanol-fixed cells stained with propidium iodide after RNase treatment. Cells from 17 samples were also exposed to [3H]-thymidine and labeling indices were determined. An excellent linear correlation was observed between the percentage of S cells calculated by flow cytometry and the labeling index (r = 0.95). To determine the relationship between the percentage of S cells and the clinical behavior of the tumors, the survival of the 27 patients was analyzed. None of the patients was undergoing antineoplastic therapy at the time of the study. The patients were separated into 2 groups according to the percentage of S cells calculated by flow cytometry: those with less than 5% S cells and those with higher than 5% S cells. Clinical follow-up ranged from 5 to 20 months. While 7 of 12 patients with ...