Dilsat Ariksoysal - Academia.edu (original) (raw)

Papers by Dilsat Ariksoysal

Nanotechnology is a term that is frequently heard over the past 20 years and it is still an inter... more Nanotechnology is a term that is frequently heard over the past 20 years and it is still an interesting area of research in science. Among nanomaterials, semiconductor quantum dots (QDs), which were first introduced as luminescent label dyes for the analysis of biomolecular interactions, have special optical properties such as high quantum yields, large Stokes shifts, and size-dependent fluorescent signaling. QDs can be functionalized with bio(nano)molecules and used for multiple sensing processes. In addition, thanks to the features listed above, QDs offer solutions to health-related problems in human life. QDs have been used for the detection of various types of targets such as protein, DNA, aptamer, cell, drugs, and heavy metals by using different analytical methodologies up to now. But nowadays, QDs have been added to the “electrochemical DNA biosensor designs” and significant progress has been made in this area. Detailed reviews and lots of research papers have been written in ...

Biosensor is a bio-analytical integrated device that uses a biological detection system to target... more Biosensor is a bio-analytical integrated device that uses a biological detection system to target molecules or macromolecules. Biosensors can provide specific quantitative and semi quantitative analytical information using biological recognition by coupling to a physiochemical transducer which converts this recognition into a detectable output signal. The characteristic features of a biosensor are linearity, sensitivity, selectivity and response time. Biosensors have potential applications beginning with medical, pharma, environmental, biodefence and food & beverage and have now extensive applications in health care. Biosensors are involved in molecular diagnosis, crime detection, drug development, food analysis, industrial process control, environmental field monitoring, detection systems in biological warfare agents and in manufacturing of pharmaceuticals and organ replacement. Biosensors have firmly established their applications in health care in the measurement of blood metabolites such as glucose, lactate, urea and creatinine, diabetes therapy, cardiovascular disease biomarkers and cancer biomarkers. This review mainly focuses on the recent advances and applications of biosensors in health care.

Food contamination is a matter of serious concern, as the high concentration of chemicals present... more Food contamination is a matter of serious concern, as the high concentration of chemicals present in edibles poses serious health risks. The development of highly sensitive methods to check for the presence of certain substances in food is therefore becoming necessary. In this context, there is a growing interest in the application of aptamer-based approaches for food analysis in response to these significant challenges. Given their unique chemical and physical properties, quantum dots (QDs) are widely used as nanosensors in several research fields. In this work, QD-based electrochemiluminescent and photoelectrochemical aptamer sensors are discussed, focusing on their specific applications on mycotoxins and pesticides as examples of food contaminants of most concern for health. The chapter covers the latest trends for food contaminant detection. The most recent approaches (within the past 4 years) are emphasized while other older but still interesting approaches are also described.

Cumhuriyet Science Journal, Mar 22, 2019

This study was designed to investigate the performance of carbon nanotubes (CNT) modified carbon ... more This study was designed to investigate the performance of carbon nanotubes (CNT) modified carbon paste and carbon printed electrodes (SPE) produced in laboratory conditions. The effect of carbon nanotube use on signal enrichment was determined by using cyclic voltammetry (CV), square wave voltammetry (SWV) or differential pulse voltammetry (DPV) techniques based on potassium ferricyanide/ ferrocyanide or guanine signal. The application of different activation procedures to the electrode surface such as chemical (H 2 SO 4 , acetone, N,N-Dimethylformamide or NaOH) or electrochemical (different potential applications) were presented in this study. It was observed that the activation procedure applied to the nanotube modified electrode has strong effects on signal enrichment. From these procedures it was determined that the guanine signal obtained in activation with NaOH increased about 62-fold. It was also found that different nanotube species gave different responses to the activation processes. The optimum conditions of the nanotube-based biosensor were also presented.

and since 2012 she has been working as an Associate Professor in the same department. She worked ... more and since 2012 she has been working as an Associate Professor in the same department. She worked and collaborated with Mehmet Ozsoz for approximately 12 years in his biosensor group. Her areas of interest include the development of enzymatic, electrochemical, optical (surface plasmon resonance) and impedimetric DNA biosensors, nanobiosensors and electrode/surface modifications for the detection of DNA hybridization and DNA damage, compound-DNA interactions, gene mutations, and DNA analysis related to microorganisms.

Electrochemical DNA Biosensors, 2012

Sensors and Actuators B: Chemical

Journal of Scientific Perspectives

The design of biosensors is one of the most important areas of analytical chemistry today, and in... more The design of biosensors is one of the most important areas of analytical chemistry today, and interest in DNA-based bio (nano) sensors developed to examine applications related to compound-DNA interaction has been increasing in the last two decades. Changing in DNA structure, even for therapeutic purposes, can have serious effects on human health. The detection of any chemical substances in DNA sutructure is very important. In this study, an anticancer drug Tamoxifen (TAM) is used in the treatment of cancer since the early 1970s was identifying the possible DNA interaction during treatment by using differential pulse voltammetry (DPV) based on both TAM and guanine oxidation signals at the disposable pencil graphite electrode (PGE). The effect of TAM on single stranded (ss)-DNA and double stranded (ds)-DNA showed differences, depending on the double helix and single stranded structure. It was found that TAM interacting to ds-DNA more strongly than ss-DNA. Thus, Drug-DNA interaction analysis has been investigated for the first time under optimized conditions with the Tamoxifen which, gave an oxidation peak potential near the guanine oxidation area. These results presented that the developed DNA biosensor could be detected TAM-DNA interaction as a sensitive, rapid and cost effective way. Electrochemical detectionTamoxifen recovery from commercial tablets was also studied..

Journal of Scientific Perspectives

The electrochemical nanobiosensor was designed for the determination of specific DNA sequences re... more The electrochemical nanobiosensor was designed for the determination of specific DNA sequences related to breast cancer 1 (BRCA1) gene and interaction between Anticancer Drug Tamoxifen (TAM) and related DNA sequences by using pencil graphite electrode (PGE), bare and multiwalled carbon nanotube (MWCNT) contained screen printed carbon electrodes (SPEs) for the first time. Here, biomolecular interaction between TAM and DNA was investigated differential pulse voltammetry (DPV) based on not only guanine signal but also TAM oxidation response. It was obtained that the guanine signal at about +1.00V obtained from probe DNA or hybrid DNA shows a remerkable increase after the interaction with TAM. Additionally, it was found that TAM interact with guanine bases and TAM signal which is near the guanine oxidation area also increase after the interaction with DNA. Consequently, the prepared biosensor offer suitable platform for the analysis of DNA hybridization and TAM-DNA interaction sensitively.

Journal of The Electrochemical Society

Talanta, 2017

This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for th... more This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for the detection of Escherichia coli (E. coli) from polymerase chain reaction (PCR)-amplified real samples while Staphylococcus aureus (S. aureus) was used to investigate the selectivity of the biosensor. The capture probe specifically recognizing E. coli DNA and it was firstly interacted with MWCNTs for wrapping of single-stranded DNA (ssDNA) onto the nanomaterial. DNA-wrapped MWCNTs were then immobilised on the surface of disposable pencil graphite electrode (PGE) for the detection of DNA hybridization. Electrochemical behaviors of the modified PGEs were investigated using Raman spectroscopy and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined and evaluated by changes in the intrinsic guanine oxidation signal at about 1.0V by DPV. Numerous factors affecting the hybridization were optimized such as target concentration, hybridization time, etc. Th...

Biosensors and their Applications in Healthcare, 2013

Combinatorial Chemistry & High Throughput Screening, 2017

Surface Plasmon Resonance (SPR) based biosensor system was developed for the detection of Delta F... more Surface Plasmon Resonance (SPR) based biosensor system was developed for the detection of Delta F508 (DF508del) Cystic Fibrosis (CF) mutation in both synthetic and real samples. In order to provide an effective hybridization between probe and the Polymerase Chain Reaction (PCR) amplicons (target), streptavidin was bound to the surface and biotin-tag probe was sent to the streptavidin-coated surface. For the target preparation, blood samples were collected from the patients who suffer from CF. Following the DNA isolation; samples were ampliﬁed with PCR with biotin-tag. Before sending the biotin-tag PCR amplicons onto the modified surface, amplicons were also interacted with the helper oligonucleotides to prevent re-annealing of the denatured DNA strands. This kind of 'multiple surface binding' method helps increasing of the sensitivity of the detection. The limit of detection(S/N= 3) was calculated as 12.24 pico-mole/ml for PCR-like synthetic long target sequence and 13x105 molecules for real samples in less than half hour. Using the both biotin-tag probe and the helper oligonucleotides together, hybridization was obtained much more efficiently than traditional denaturation protocols for real samples and biotin-free hybridization detection. To the best of our knowledge, the procedure described in this study is one of the simplest, rapid and sensitive methods for CF mutation detection with SPR based biosensor system in real samples.

2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2001

INTRODUCTION Nucleic acids have become the ultimate tools in the recognition and monitoring of ma... more INTRODUCTION Nucleic acids have become the ultimate tools in the recognition and monitoring of many important compounds [1]. There is a great demand for a detection system which cannot only determine specific DNA fragments, but can also determine the exact total nucleic acid content of a sample. For more than a decade, DNA biosensor technologies are under intense investigation owing to their great promise for rapid and low-cost detection of specific DNA sequences in human, viral and bacterial nucleic acids [2]. Recently, some electrochemical techniques that are well suited for detecting hybridization and DNA damage events have been reported. Hybridization can be detected by redox-active metal comple xes that associate selectively and reversibly with double stranded immobilized DNA [3,4]. Erdem et al. [5,6] reported that methylene blue (MB) could be used as a redox-active indicator for the electrochemical detection of mismatched bases in DNA. Only a little attention has been paid to the development of electrochemical DNA biosensors that do not require an external redox indicator. Wang et al. [7] described an indicator-free electrochemical DNA biosensor protocol, which involves the immobilization of inosine-substituted (guanine-free) probe onto CPE and the detection of hybrid formation was performed by using the appearance of the guanine oxidation signal of the target in connection with chronopotentiometric stripping analysis (PSA). Napier et al. [8] also used inosine substituted probes, which still recognized cytidine but not donated an electron to [Ru(bpy) 3 ] 2+. Their hybridization indicator, [Ru(bpy) 3 ] 2+ , exhibited a reversible redox couple at 1.05 V and oxidized guanine in the products of polymerase chain reaction (PCR) of genomic material from infectious organisms. Methylene blue (MB) is an aromatic heterocycle that binds strongly to DNA via intercalation. MB interacts in a different way with ssDNA and dsDNA. The decreased electrochemical response due to the association of the large planar hydrophobic phenothiazine group with the surface duplex thus serves as the DNA recognition signal [5,6]. In particular, the cationic charge of MB would improve the DNA binding affinity electrostatic interaction with phosphate backbone. Barton and co-workers [9] reported on a gold electrode derivatized with DNA oligonucleotides containing a pendant 5'-hexanethiol linker. They used this electrode to detect the point mutations in DNA by using the electrocatalyzis of the MB, which is intercalated into the duplex at the electrode surface. Here we describe an electrochemical DNA biosensor for the detection of DNA hybridization using the oxidation signals of guanine and adenine and the reduction signals of MB in connection with DPV. EXPERIMENTAL SECTION Apparatus The oxidation signals of adenine and guanine were investigated by using DPV with an AUTOLAB PGSTAT 30 electrochemical analysis system and GPES 4.8 software package (Eco Chemie, The Netherlands). The three electrode system, consisted of the in-house made carbon paste electrode (CPE) as the working electrode, the reference electrode (Ag/AgCl) and a platinum wire as the auxiliary electrode. The body of CPE was a glass tube (3 mm i.d.) tightly packed with the carbon pa ste. The electrical contact was provided by a copper wire inserted into the carbon paste. Carbon paste was prepared in the usual way by hand-mixing graphite powder (Fisher) and mineral oil (Acheson 38) in a 70:30 mass ratio. The surface was polished on a weighing paper to a smoothed finish before use. The convective transport was provided by a magnetic stirrer.

Background: Glycopeptide-resistant enterococci (GRE) have emerged as a significant cause of nosoc... more Background: Glycopeptide-resistant enterococci (GRE) have emerged as a significant cause of nosocomial infections worldwide due to widespread use of glycopeptide antimicrobial drugs such as vancomycin, teicoplanin in hospitals and avorporcin as a growth promoter in poultry industry. A fast and reliable on-site monitoring scheme is required for quick preventive action and early diagnosis. Hence we have developed a rapid geno-chromatography biosensor assay for the identification of the high-level vancomycin- and teicoplanin-resistance gene, vanA. Methods: vanA PCR was performed with biotin- and fluorescein-labeled primers. A redox current signal was generated by the action of peroxidase-conjugated anti-fluorescein antibody with hydrogen peroxide and was detected using a combination of glass membrane filter chromatography and electrochemical DNA biosensor on a portable amperometer. An internal control was incorporated for assay reliability. The specificity and sensitivity was evaluated...

Clinical chemistry, 2002

Improved stable isotope dilution-gas chromatography-mass spectrometry method for serum or plasma ... more Improved stable isotope dilution-gas chromatography-mass spectrometry method for serum or plasma free 3-hydroxy-fatty acids and its utility for the study of disorders of mitochondrial fatty acid ␤-oxidation. Clin Chem 2000; 46:149-55. 5. Landini D, Montanari F, Rolla F. Selective oxidation of primary alcohols to aldehydes under phase transfer conditions. Synthesis 1979;2:134-6. 6. Jones PM, Burlina AB, Bennett MJ. Quantitative measurement of total and free 3-hydroxy fatty acids in serum or plasma samples: short-chain 3-hydroxy fatty acids are not esterified.

Langmuir, 2003

A glassy carbon electrode (GCE) and a single-use pencil graphite electrode (PGE) modified through... more A glassy carbon electrode (GCE) and a single-use pencil graphite electrode (PGE) modified through the incorporation of a mixed-ion, amphiphilic, fluorohectorite heterostructure, wherein the galleries are regularly interstratified by hydrophilic inorganic ions (Na + ) and ...

Cumhuriyet Science Journal, Mar 22, 2019

Electrochemical DNA Biosensors, 2012

Sensors and Actuators B: Chemical

Journal of Scientific Perspectives

Journal of The Electrochemical Society

Talanta, 2017

Biosensors and their Applications in Healthcare, 2013

Combinatorial Chemistry & High Throughput Screening, 2017

2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2001

Clinical chemistry, 2002

Langmuir, 2003