Donato Zipeto - Academia.edu (original) (raw)
Uploads
Papers by Donato Zipeto
Additional file 1: SARS-CoV-2 % neutralization for the International Standard for anti-SARS-CoV-2... more Additional file 1: SARS-CoV-2 % neutralization for the International Standard for anti-SARS-CoV-2 antibody and Reference Panel members. Normalized percentage neutralization values are plotted against the logarithm of the dilution factors for the International Standard for anti-SARS-CoV-2 antibody (NIBSC code 20/136) and WHO Reference Panel: 20/150 (High titre, H), 20/148 ( Mid titre, M), 20/140 (Low titre, L), 20/142 (negative, N)
Kinetics of transcription of human cytomegalovirus chemokine
Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal ... more Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal cancer progression has been inferred by the identification in tumour tissues of HCMV antigens and specific viral DNA or RNA sequences. To further investigate the relationship between HCMV and colorectal cancers we developed qualitative and quantitative PCR assay to detect HCMV DNA in 56 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients belonging to 4 different histological phenotypes: adenoma; poorly, moderately and well differentiated adenocarcinomas. Results: Of the 56 FFPE tested tissue samples, 6 (11%) were positive for HCMV nested PCR amplification, and more precisely 1 (5%) of 20 cases of adenoma and 5 (21%) of 24 cases of moderately differentiated adenocarcinoma. No PCR positivity was obtained in samples from well and poorly differentiated adenocarcinomas. Conclusion: Our observations suggest that there is no evidence of a direct association between HCMV and col...
Journal of Translational Medicine, 2022
Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and seve... more Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and severe outcomes. In vaccinated subjects with SARS-CoV-2 history, RBD-specific IgG and pseudovirus neutralization titers were rapidly recalled by a single BTN162b2 vaccine dose to higher levels than those in naïve recipients after the second dose, irrespective of waning immunity. In this study, we inspected the long-term kinetic and neutralizing responses of S-specific IgG induced by two administrations of BTN162b2 vaccine in infection-naïve subjects and in subjects previously infected with SARS-CoV-2. Methods Twenty-six naïve and 9 previously SARS-CoV-2 infected subjects during the second wave of the pandemic in Italy were enrolled for this study. The two groups had comparable demographic and clinical characteristics. By means of ELISA and pseudotyped-neutralization assays, we investigated the kinetics of developed IgG-RBD and their neutralizing activity against both the ancestral D614G and ...
Journal of Translational Medicine, 2022
Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and seve... more Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and severe outcomes. In vaccinated subjects with SARS-CoV-2 history, RBD-specific IgG and pseudovirus neutralization titers were rapidly recalled by a single BTN162b2 vaccine dose to higher levels than those in naïve recipients after the second dose, irrespective of waning immunity. In this study, we inspected the long-term kinetic and neutralizing responses of S-specific IgG induced by two administrations of BTN162b2 vaccine in infection-naïve subjects and in subjects previously infected with SARS-CoV-2. Methods Twenty-six naïve and 9 previously SARS-CoV-2 infected subjects during the second wave of the pandemic in Italy were enrolled for this study. The two groups had comparable demographic and clinical characteristics. By means of ELISA and pseudotyped-neutralization assays, we investigated the kinetics of developed IgG-RBD and their neutralizing activity against both the ancestral D614G and ...
HLA, 2020
Surface expression of HLA-class I molecules is critical for modulating T/NK lymphocytes' effe... more Surface expression of HLA-class I molecules is critical for modulating T/NK lymphocytes' effector functions. Amongst HLA molecules, HLA-C, the most recently evolved form of class I antigens, is subjected to both transcriptional and multiple post transcriptional regulation mechanisms affecting its cell surface expression. Amongst the latter a region placed in the 3' untranslated region (UTR) of HLA-C transcript contains the single nucleotide polymorphism (SNP) rs67384697 "G-ins/del" that has been found to be strictly associated with surface levels of HLA-C allomorphs due to the effect on the binding site of a microRNA (Hsa-miR-148a). Higher expression of HLA-C has proved to influence HIV-1 infection via a better control of viremia and a slower disease progression. More importantly, the analysis of SNP rs67384697 "G-ins/del" combined with the evaluation of the HLA-Bw4/-Bw6 C1/C2 supratype, as well as the KIR genetic asset, has proved to be pivotal in defining the status of Elite Controllers in the caucasian population. Here we describe a new reliable and fast method of allele-specific real-time PCR to monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a in a high-throughput format that can be easily applied to studies involving large cohorts of individuals. This article is protected by copyright. All rights reserved.
Il ruolo di HLA-C nell'infezione da HIV-
Scientific Reports, 2021
The GNA15 gene is ectopically expressed in human pancreatic ductal adenocarcinoma cancer cells. T... more The GNA15 gene is ectopically expressed in human pancreatic ductal adenocarcinoma cancer cells. The encoded Gα15 protein can promiscuously redirect GPCR signaling toward pathways with oncogenic potential. We sought to describe the distribution of GNA15 in adenocarcinoma from human pancreatic specimens and to analyze the mechanism driving abnormal expression and the consequences on signaling and clinical follow-up. We detected GNA15 expression in pre-neoplastic pancreatic lesions and throughout progression. The analysis of biological data sets, primary and xenografted human tumor samples, and clinical follow-up shows that elevated expression is associated with poor prognosis for GNA15, but not any other GNA gene. Demethylation of the 5′ GNA15 promoter region was associated with ectopic expression of Gα15 in pancreatic neoplastic cells, but not in adjacent dysplastic or non-transformed tissue. Down-modulation of Gα15 by shRNA or CRISPR/Cas9 affected oncogenic signaling, and reduced ad...
Additional file 1: SARS-CoV-2 % neutralization for the International Standard for anti-SARS-CoV-2... more Additional file 1: SARS-CoV-2 % neutralization for the International Standard for anti-SARS-CoV-2 antibody and Reference Panel members. Normalized percentage neutralization values are plotted against the logarithm of the dilution factors for the International Standard for anti-SARS-CoV-2 antibody (NIBSC code 20/136) and WHO Reference Panel: 20/150 (High titre, H), 20/148 ( Mid titre, M), 20/140 (Low titre, L), 20/142 (negative, N)
Kinetics of transcription of human cytomegalovirus chemokine
Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal ... more Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal cancer progression has been inferred by the identification in tumour tissues of HCMV antigens and specific viral DNA or RNA sequences. To further investigate the relationship between HCMV and colorectal cancers we developed qualitative and quantitative PCR assay to detect HCMV DNA in 56 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients belonging to 4 different histological phenotypes: adenoma; poorly, moderately and well differentiated adenocarcinomas. Results: Of the 56 FFPE tested tissue samples, 6 (11%) were positive for HCMV nested PCR amplification, and more precisely 1 (5%) of 20 cases of adenoma and 5 (21%) of 24 cases of moderately differentiated adenocarcinoma. No PCR positivity was obtained in samples from well and poorly differentiated adenocarcinomas. Conclusion: Our observations suggest that there is no evidence of a direct association between HCMV and col...
Journal of Translational Medicine, 2022
Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and seve... more Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and severe outcomes. In vaccinated subjects with SARS-CoV-2 history, RBD-specific IgG and pseudovirus neutralization titers were rapidly recalled by a single BTN162b2 vaccine dose to higher levels than those in naïve recipients after the second dose, irrespective of waning immunity. In this study, we inspected the long-term kinetic and neutralizing responses of S-specific IgG induced by two administrations of BTN162b2 vaccine in infection-naïve subjects and in subjects previously infected with SARS-CoV-2. Methods Twenty-six naïve and 9 previously SARS-CoV-2 infected subjects during the second wave of the pandemic in Italy were enrolled for this study. The two groups had comparable demographic and clinical characteristics. By means of ELISA and pseudotyped-neutralization assays, we investigated the kinetics of developed IgG-RBD and their neutralizing activity against both the ancestral D614G and ...
Journal of Translational Medicine, 2022
Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and seve... more Background COVID-19 vaccines have demonstrated effectiveness in reducing SARS-CoV-2 mild and severe outcomes. In vaccinated subjects with SARS-CoV-2 history, RBD-specific IgG and pseudovirus neutralization titers were rapidly recalled by a single BTN162b2 vaccine dose to higher levels than those in naïve recipients after the second dose, irrespective of waning immunity. In this study, we inspected the long-term kinetic and neutralizing responses of S-specific IgG induced by two administrations of BTN162b2 vaccine in infection-naïve subjects and in subjects previously infected with SARS-CoV-2. Methods Twenty-six naïve and 9 previously SARS-CoV-2 infected subjects during the second wave of the pandemic in Italy were enrolled for this study. The two groups had comparable demographic and clinical characteristics. By means of ELISA and pseudotyped-neutralization assays, we investigated the kinetics of developed IgG-RBD and their neutralizing activity against both the ancestral D614G and ...
HLA, 2020
Surface expression of HLA-class I molecules is critical for modulating T/NK lymphocytes' effe... more Surface expression of HLA-class I molecules is critical for modulating T/NK lymphocytes' effector functions. Amongst HLA molecules, HLA-C, the most recently evolved form of class I antigens, is subjected to both transcriptional and multiple post transcriptional regulation mechanisms affecting its cell surface expression. Amongst the latter a region placed in the 3' untranslated region (UTR) of HLA-C transcript contains the single nucleotide polymorphism (SNP) rs67384697 "G-ins/del" that has been found to be strictly associated with surface levels of HLA-C allomorphs due to the effect on the binding site of a microRNA (Hsa-miR-148a). Higher expression of HLA-C has proved to influence HIV-1 infection via a better control of viremia and a slower disease progression. More importantly, the analysis of SNP rs67384697 "G-ins/del" combined with the evaluation of the HLA-Bw4/-Bw6 C1/C2 supratype, as well as the KIR genetic asset, has proved to be pivotal in defining the status of Elite Controllers in the caucasian population. Here we describe a new reliable and fast method of allele-specific real-time PCR to monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a in a high-throughput format that can be easily applied to studies involving large cohorts of individuals. This article is protected by copyright. All rights reserved.
Il ruolo di HLA-C nell'infezione da HIV-
Scientific Reports, 2021
The GNA15 gene is ectopically expressed in human pancreatic ductal adenocarcinoma cancer cells. T... more The GNA15 gene is ectopically expressed in human pancreatic ductal adenocarcinoma cancer cells. The encoded Gα15 protein can promiscuously redirect GPCR signaling toward pathways with oncogenic potential. We sought to describe the distribution of GNA15 in adenocarcinoma from human pancreatic specimens and to analyze the mechanism driving abnormal expression and the consequences on signaling and clinical follow-up. We detected GNA15 expression in pre-neoplastic pancreatic lesions and throughout progression. The analysis of biological data sets, primary and xenografted human tumor samples, and clinical follow-up shows that elevated expression is associated with poor prognosis for GNA15, but not any other GNA gene. Demethylation of the 5′ GNA15 promoter region was associated with ectopic expression of Gα15 in pancreatic neoplastic cells, but not in adjacent dysplastic or non-transformed tissue. Down-modulation of Gα15 by shRNA or CRISPR/Cas9 affected oncogenic signaling, and reduced ad...