Dorothée Poidatz - Academia.edu (original) (raw)

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Papers by Dorothée Poidatz

Placenta, Sep 1, 2012

Placenta growth and functions depend on correct trophoblast migration, proliferation, and differe... more Placenta growth and functions depend on correct trophoblast migration, proliferation, and differentiation. The placenta has a critical role in gas and nutrient transport. To accomplish these numerous functions, the placenta depends on a highly efficient energy metabolism control. Recent studies showed that the orphan nuclear receptor Estrogen-Related Receptor gamma (ERRg) is highly expressed in human placentas. As ERRg has been described as a major energy metabolism regulator, we investigated ERRg expression and putative roles on energy homeostasis in human trophoblast from first trimester placentas. First, we showed that ERRg expression level increased during pregnancy and that ERRg was more abundant in villous than in extravillous trophoblasts. We also observed that ERRg expression increased during trophoblast differentiation. Second, we demonstrated that mitochondrial biogenesis and expression of some energy metabolism target genes decreased when ERRg expression was impaired. Altogether, these results suggest that ERRg could be implicated in the energy metabolism regulation of human trophoblasts.

Le placenta humain est un organe indispensable au maintien de la grossesse et au développement fœ... more Le placenta humain est un organe indispensable au maintien de la grossesse et au développement fœtal. Son unité structurale et fonctionnelle est la villosité choriale, constituée principalement de cytotrophoblastes qui se différencient selon la voie villeuse endocrine ou extravilleuse invasive. Le développement intense du placenta et ses fonctions multiples requièrent des besoins en énergie importants. La régulation du métabolisme énergétique placentaire passe, en partie, par le contrôle de l’activité mitochondriale.La mitochondrie est l’organelle clé du métabolisme énergétique. Cependant, elle intervient dans de nombreuses autres fonctions cellulaires telles que l’apoptose et la biosynthèse des hormones stéroïdes. Des études récentes suggèrent que les mitochondries sont impliquées dans la différenciation cellulaire.Le récepteur apparenté aux récepteurs des oestrogènes-γ, (ERRγ), est un facteur de transcription qui joue un rôle crucial dans le contrôle du métabolisme énergétique. De...

Le placenta humain est un organe indispensable au maintien de la grossesse et au developpement fœ... more Le placenta humain est un organe indispensable au maintien de la grossesse et au developpement fœtal. Son unite structurale et fonctionnelle est la villosite choriale, constituee principalement de cytotrophoblastes qui se differencient selon la voie villeuse endocrine ou extravilleuse invasive. Le developpement intense du placenta et ses fonctions multiples requierent des besoins en energie importants. La regulation du metabolisme energetique placentaire passe, en partie, par le controle de l’activite mitochondriale.La mitochondrie est l’organelle cle du metabolisme energetique. Cependant, elle intervient dans de nombreuses autres fonctions cellulaires telles que l’apoptose et la biosynthese des hormones steroides. Des etudes recentes suggerent que les mitochondries sont impliquees dans la differenciation cellulaire.Le recepteur apparente aux recepteurs des oestrogenes-γ, (ERRγ), est un facteur de transcription qui joue un role crucial dans le controle du metabolisme energetique. De...

Biology of reproduction, May 30, 2016

The placenta exchanges nutrients between the mother and the fetus, and requires a constant abunda... more The placenta exchanges nutrients between the mother and the fetus, and requires a constant abundant energy supply. Adiponectin (a cytokine produced primarily by adipose tissue) controls glucose and lipid homeostasis. It is well known that maternal serum adiponectin levels are inversely related to birth weight suggesting that adiponectin has a negative effect on fetal growth. This effect appears to be related to the control of nutrient transporters in human placenta. However, the underlying molecular mechanisms have not yet been characterized. In the present work, we studied adiponectin's direct effect on human primary cytotrophoblasts from first-trimester placenta. Our result showed that in placental cells, adiponectin (i) inhibits the expression of the major glucose transporters (GLUT1 and GLUT12) and sodium-coupled neutral amino acid transporters (SNAT1, SNAT2, and SNAT4), (ii) enhances total ATP production but decreases lactate production, (iii) inhibits mitochondrial biogene...

Placenta, 2012

Placenta growth and functions depend on correct trophoblast migration, proliferation, and differe... more Placenta growth and functions depend on correct trophoblast migration, proliferation, and differentiation. The placenta has a critical role in gas and nutrient transport. To accomplish these numerous functions, the placenta depends on a highly efficient energy metabolism control. Recent studies showed that the orphan nuclear receptor Estrogen-Related Receptor gamma (ERRg) is highly expressed in human placentas. As ERRg has been described as a major energy metabolism regulator, we investigated ERRg expression and putative roles on energy homeostasis in human trophoblast from first trimester placentas. First, we showed that ERRg expression level increased during pregnancy and that ERRg was more abundant in villous than in extravillous trophoblasts. We also observed that ERRg expression increased during trophoblast differentiation. Second, we demonstrated that mitochondrial biogenesis and expression of some energy metabolism target genes decreased when ERRg expression was impaired. Altogether, these results suggest that ERRg could be implicated in the energy metabolism regulation of human trophoblasts.

Fertility and sterility, Jan 6, 2015

To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major... more To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major inducer of mitochondrial biogenesis) in placentas from women with intrauterine growth restriction (IUGR) associated or not with pre-eclampsia (PE), relative to control placentas. Case-control study. Teaching hospital and university research laboratory. Thirty-nine placentas from women with IUGR, 8 IUGR+PE, and 30 controls. None. Mitochondrial DNA and protein content, gene and protein expression. We observed significantly lower placental mitochondrial DNA and protein contents (associated with down-regulation of ERRγ expression) in IUGR and IUGR+PE placentas, relative to control placentas. Our results also revealed that the placental mitochondrial DNA content was directly correlated with fetal weight. Moreover, we observed significantly lower peroxisome proliferator-activated receptor-γ coactivator-1α and sirtuin 1 messenger RNA expression levels in IUGR+PE placentas, relative to control ...

Placenta, Sep 1, 2012

Placenta growth and functions depend on correct trophoblast migration, proliferation, and differe... more Placenta growth and functions depend on correct trophoblast migration, proliferation, and differentiation. The placenta has a critical role in gas and nutrient transport. To accomplish these numerous functions, the placenta depends on a highly efficient energy metabolism control. Recent studies showed that the orphan nuclear receptor Estrogen-Related Receptor gamma (ERRg) is highly expressed in human placentas. As ERRg has been described as a major energy metabolism regulator, we investigated ERRg expression and putative roles on energy homeostasis in human trophoblast from first trimester placentas. First, we showed that ERRg expression level increased during pregnancy and that ERRg was more abundant in villous than in extravillous trophoblasts. We also observed that ERRg expression increased during trophoblast differentiation. Second, we demonstrated that mitochondrial biogenesis and expression of some energy metabolism target genes decreased when ERRg expression was impaired. Altogether, these results suggest that ERRg could be implicated in the energy metabolism regulation of human trophoblasts.

Le placenta humain est un organe indispensable au maintien de la grossesse et au développement fœ... more Le placenta humain est un organe indispensable au maintien de la grossesse et au développement fœtal. Son unité structurale et fonctionnelle est la villosité choriale, constituée principalement de cytotrophoblastes qui se différencient selon la voie villeuse endocrine ou extravilleuse invasive. Le développement intense du placenta et ses fonctions multiples requièrent des besoins en énergie importants. La régulation du métabolisme énergétique placentaire passe, en partie, par le contrôle de l’activité mitochondriale.La mitochondrie est l’organelle clé du métabolisme énergétique. Cependant, elle intervient dans de nombreuses autres fonctions cellulaires telles que l’apoptose et la biosynthèse des hormones stéroïdes. Des études récentes suggèrent que les mitochondries sont impliquées dans la différenciation cellulaire.Le récepteur apparenté aux récepteurs des oestrogènes-γ, (ERRγ), est un facteur de transcription qui joue un rôle crucial dans le contrôle du métabolisme énergétique. De...

Le placenta humain est un organe indispensable au maintien de la grossesse et au developpement fœ... more Le placenta humain est un organe indispensable au maintien de la grossesse et au developpement fœtal. Son unite structurale et fonctionnelle est la villosite choriale, constituee principalement de cytotrophoblastes qui se differencient selon la voie villeuse endocrine ou extravilleuse invasive. Le developpement intense du placenta et ses fonctions multiples requierent des besoins en energie importants. La regulation du metabolisme energetique placentaire passe, en partie, par le controle de l’activite mitochondriale.La mitochondrie est l’organelle cle du metabolisme energetique. Cependant, elle intervient dans de nombreuses autres fonctions cellulaires telles que l’apoptose et la biosynthese des hormones steroides. Des etudes recentes suggerent que les mitochondries sont impliquees dans la differenciation cellulaire.Le recepteur apparente aux recepteurs des oestrogenes-γ, (ERRγ), est un facteur de transcription qui joue un role crucial dans le controle du metabolisme energetique. De...

Biology of reproduction, May 30, 2016

The placenta exchanges nutrients between the mother and the fetus, and requires a constant abunda... more The placenta exchanges nutrients between the mother and the fetus, and requires a constant abundant energy supply. Adiponectin (a cytokine produced primarily by adipose tissue) controls glucose and lipid homeostasis. It is well known that maternal serum adiponectin levels are inversely related to birth weight suggesting that adiponectin has a negative effect on fetal growth. This effect appears to be related to the control of nutrient transporters in human placenta. However, the underlying molecular mechanisms have not yet been characterized. In the present work, we studied adiponectin's direct effect on human primary cytotrophoblasts from first-trimester placenta. Our result showed that in placental cells, adiponectin (i) inhibits the expression of the major glucose transporters (GLUT1 and GLUT12) and sodium-coupled neutral amino acid transporters (SNAT1, SNAT2, and SNAT4), (ii) enhances total ATP production but decreases lactate production, (iii) inhibits mitochondrial biogene...

Placenta, 2012

Placenta growth and functions depend on correct trophoblast migration, proliferation, and differe... more Placenta growth and functions depend on correct trophoblast migration, proliferation, and differentiation. The placenta has a critical role in gas and nutrient transport. To accomplish these numerous functions, the placenta depends on a highly efficient energy metabolism control. Recent studies showed that the orphan nuclear receptor Estrogen-Related Receptor gamma (ERRg) is highly expressed in human placentas. As ERRg has been described as a major energy metabolism regulator, we investigated ERRg expression and putative roles on energy homeostasis in human trophoblast from first trimester placentas. First, we showed that ERRg expression level increased during pregnancy and that ERRg was more abundant in villous than in extravillous trophoblasts. We also observed that ERRg expression increased during trophoblast differentiation. Second, we demonstrated that mitochondrial biogenesis and expression of some energy metabolism target genes decreased when ERRg expression was impaired. Altogether, these results suggest that ERRg could be implicated in the energy metabolism regulation of human trophoblasts.

Fertility and sterility, Jan 6, 2015

To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major... more To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major inducer of mitochondrial biogenesis) in placentas from women with intrauterine growth restriction (IUGR) associated or not with pre-eclampsia (PE), relative to control placentas. Case-control study. Teaching hospital and university research laboratory. Thirty-nine placentas from women with IUGR, 8 IUGR+PE, and 30 controls. None. Mitochondrial DNA and protein content, gene and protein expression. We observed significantly lower placental mitochondrial DNA and protein contents (associated with down-regulation of ERRγ expression) in IUGR and IUGR+PE placentas, relative to control placentas. Our results also revealed that the placental mitochondrial DNA content was directly correlated with fetal weight. Moreover, we observed significantly lower peroxisome proliferator-activated receptor-γ coactivator-1α and sirtuin 1 messenger RNA expression levels in IUGR+PE placentas, relative to control ...