Dr.Samsudin Cassim - Academia.edu (original) (raw)
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Papers by Dr.Samsudin Cassim
Tissue Engineering and Regenerative Medicine, 2011
In this study, we are taking step to actively manage osteoarthritis that may help gain control ov... more In this study, we are taking step to actively manage osteoarthritis that may help gain control over osteoarthritic pain and delay the degenerative changes in articular cartilage in future. We transiently over expressed cartilage transcriptional factor, human sox-9 gene in chondrocytes derived from consented osteoarthritic patients after joint surgery. The expression vector carrying human sox-9 gene, pAdTrack-sox9 was transformed into One Shot TOP 10 Chemically Competent E. coli according to the manufacturer protocol. Plasmid purification was performed in accordance with QIAGEN (R) plasmid purification kit procedure. We compared the efficiency between two transfection techniques i.e. lipofection using Lipofectamine (TM) 2000 kit from Invitrogen, USA and nucleofection using Human Chondrocytes Nucleofector (R) kit from Amaxa Biosystem, Germany. Chondrocytes were cultured and transfected with sox-9 gene at passage 1 according to the manufacturers' protocols. Transfected chondrocytes...
This study was designed to verify the optimal · basic culture media that promote chondrocytes pro... more This study was designed to verify the optimal · basic culture media that promote chondrocytes proliferation in vitro in order to facilitate adequate amount of chondrocytes for cartilage reconstructionas well as maintaining cartilage specific phenotype. Human articular chondrocytes were cultured in three types of basic culture media Ham`s F12, DMEM and the equivalent mixture of F12:DMEM. Cultured chondrocytes were trypsinized as they reached confluency. The viability and total number of cell were recorded at every passage. Large-scale culture expansion was used to reconstruct tissue-engineered cartilage. Quantitative RT-PCR analysis was used to evaluate the expression of collagen Type II, collagen Type I, aggrecan and Sox 9 gene, both in monolayer culture and in the engineered cartilage. The mixture of F12:DMEM promotes significantly greater (p<0.05) chondrocytes proliferation at every passage compared to the individual medium. Monolayer cultured chondrocytes exhibited down-regula...
We report a case of a man presenting with painless diffuse swelling over his right knee region wi... more We report a case of a man presenting with painless diffuse swelling over his right knee region with the clinical and radiological features suggesting a benign soft tissue tumour. Surgical removal of the lesion was done. A histopathological (HPE) and polymerase chain reaction (PCR) examination for Mycobacterium tuberculosis DNA confirmed the diagnosis of tuberculous bursitis around the knee with joint involvement. He was treated with 6 months of antituberculous chemotherapy. Correct diagnosis was difficult and delayed because of the rarity of the condition. Modern imaging technology including magnetic resonance imaging, in particular, was not conclusive.
Tissue Engineering and Regenerative Medicine, 2011
In this study, we are taking step to actively manage osteoarthritis that may help gain control ov... more In this study, we are taking step to actively manage osteoarthritis that may help gain control over osteoarthritic pain and delay the degenerative changes in articular cartilage in future. We transiently over expressed cartilage transcriptional factor, human sox-9 gene in chondrocytes derived from consented osteoarthritic patients after joint surgery. The expression vector carrying human sox-9 gene, pAdTrack-sox9 was transformed into One Shot TOP 10 Chemically Competent E. coli according to the manufacturer protocol. Plasmid purification was performed in accordance with QIAGEN (R) plasmid purification kit procedure. We compared the efficiency between two transfection techniques i.e. lipofection using Lipofectamine (TM) 2000 kit from Invitrogen, USA and nucleofection using Human Chondrocytes Nucleofector (R) kit from Amaxa Biosystem, Germany. Chondrocytes were cultured and transfected with sox-9 gene at passage 1 according to the manufacturers' protocols. Transfected chondrocytes...
This study was designed to verify the optimal · basic culture media that promote chondrocytes pro... more This study was designed to verify the optimal · basic culture media that promote chondrocytes proliferation in vitro in order to facilitate adequate amount of chondrocytes for cartilage reconstructionas well as maintaining cartilage specific phenotype. Human articular chondrocytes were cultured in three types of basic culture media Ham`s F12, DMEM and the equivalent mixture of F12:DMEM. Cultured chondrocytes were trypsinized as they reached confluency. The viability and total number of cell were recorded at every passage. Large-scale culture expansion was used to reconstruct tissue-engineered cartilage. Quantitative RT-PCR analysis was used to evaluate the expression of collagen Type II, collagen Type I, aggrecan and Sox 9 gene, both in monolayer culture and in the engineered cartilage. The mixture of F12:DMEM promotes significantly greater (p<0.05) chondrocytes proliferation at every passage compared to the individual medium. Monolayer cultured chondrocytes exhibited down-regula...
We report a case of a man presenting with painless diffuse swelling over his right knee region wi... more We report a case of a man presenting with painless diffuse swelling over his right knee region with the clinical and radiological features suggesting a benign soft tissue tumour. Surgical removal of the lesion was done. A histopathological (HPE) and polymerase chain reaction (PCR) examination for Mycobacterium tuberculosis DNA confirmed the diagnosis of tuberculous bursitis around the knee with joint involvement. He was treated with 6 months of antituberculous chemotherapy. Correct diagnosis was difficult and delayed because of the rarity of the condition. Modern imaging technology including magnetic resonance imaging, in particular, was not conclusive.