Dusanka Kitic - Academia.edu (original) (raw)

Papers by Dusanka Kitic

Journal of Essential Oil Research, Mar 1, 2002

ABSTRACT An oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball obtained by hydr... more ABSTRACT An oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball obtained by hydrodistillation was analyzed by GC and GC/MS and 36 constituents were identified (98.4%). The main constituents in the oil were pulegone (37.5%), menthone (17,6%), piperitenone (15.0%) and piperitone (10.2%).The antimicrobial activity of the oil was screened against Aspergillus niger, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Bacillus subtilis and Pseudomonas aeruginosa. The microorganisms were found to be susceptible to the oil

Lekovite sirovine, 2022

Birch leaves and bark are traditionally used for folk medicine for centuries. Numerous research f... more Birch leaves and bark are traditionally used for folk medicine for centuries. Numerous research focused on the chemical composition and biological effects of birch extracts. The main pharmacologically active compounds found in extracts are flavonoids, saponins, tannins, essential oil, and triterpenes. Different species, age of the plant, geographical region, extraction methods, and analytical techniques influence the chemical composition. Possible role of birch extracts in preventing and treating numerous diseases is enabled by their diuretic, antimicrobial, anti-inflammatory, and anti-allergic activity. Therefore, this article is intended to give a general overview of the studies on birch leaves and bark.

Acta Medica Medianae, Dec 15, 2016

Berries have become inevitable part of the diet of conscious men. Even though berries are not the... more Berries have become inevitable part of the diet of conscious men. Even though berries are not the richest source of zinc, there are agricultural practices that increase zinc content in surface soils. Being markedly involved in the human physiology and biochemistry, zinc is also a common component of plant metabolic pathways. A zinc defficiency occurs in plants mostly in calcareous and alkaline soils, affecting young leaves first. The toxicity and intolerence of zinc, which occur in the soils with prolonged use of fertilizers containing zinc among other elements, are greater problems than zinc deficiency. Zinc toxicity is often associated with the deficiency of magnesium, iron or manganese. In humans, zinc deficiency leads to acrodermatitis enteropathica, growth retardation, hypogonadism, depressed mental function, impaired cognitive functions and immune disorders, affecting males and females in the developing world. Serum zinc was correlated with the severity of depression and low serum zinc levels were found in depressed patients, suggesting that serum zinc could be the marker of depression. Diverse neurodegenerative processes, as Alzheimer's disease, may change the cellular zinc level, raising it to the level where zinc contributes to the progression of the disease. Zinc plays a pivotal role in few signal transduction and gene expression pathways, including that of cytokine genes. Zinc also holds a key position for multiple functions of cell metabolism, retinal development and specific retinal functions. Zinc in therapeutic dosages was effective in decreasing the incidence of infections. Zinc lozenges reduced the duration and severity of the common cold.

Acta Facultatis Medicae Naissensis, 2023

Lekovite sirovine, 2022

The term "phytopreparations" indicates products with active substances obtained from medicinal pl... more The term "phytopreparations" indicates products with active substances obtained from medicinal plants. Herbal medicines, extracts, essential oils, and isolated active principles are present in both cosmetology and dermatology. The new term, "cosmeceutical" refers to cosmetic preparations which also have therapeutic action. A review of relevant literature and legal frames has been done in order to understand the modern use of medicinal herbs in dermatological disorders. There is no single legal framework in this area. In the therapeutic field of skin disorders and minor wounds, there are 31 European Union monographs on herbal medicinal products prepared by the Committee on Herbal Medicinal Products, while the European Scientific Cooperative on Phytotherapy recommends the use of 19 herbal drugs for dermatological disorders, such as acne, dermatitis, eczema, wounds, skin inflammation, insect bites, etc. Most patients assume an herbal product is "safe" and without side effects simply because it's natural. The rational use of herbal cosmetic products requires appropriate knowledge of used plant materials and potential side effects.

South African Journal of Botany, Nov 1, 2022

Separation and Purification Technology, 2022

Central European Journal of Biology, Jul 1, 2014

The present study investigates the chemical compositions of three Satureja montana L. ssp montana... more The present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100-500-800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.

Biologica Nyssana, Dec 29, 2018

Family Rutaceae is presented in Serbian flora with 3 genera and 4 species: Ruta graveolens L., Di... more Family Rutaceae is presented in Serbian flora with 3 genera and 4 species: Ruta graveolens L., Dictamnus albus L., Haplophyllum suaveolens (DC.) G. Don and H. boissieranum Vis. & Pančić. Comparative study of the phytochemical variability and antioxidant activity of ethanolic and methanolic extracts, obtained from these species' areal parts, was performed. Total flavonoid content was determined spectrophotometrically and HPLC method was employed for quantification of coumarins. Fast antioxidant screening of samples was done in DPPH test. Flavonoid content of the tested samples varied from 2.82±0.27 to 27.72±0.15 mg of rutin equivalent (Ru)/g of extract. Psoralen, bergapten and xanthotoxin were detected and quantified only in R. graveolens extracts. All the tested samples exhibited notable potential in DPPH radicals scavenging assay. Yet, considerably lower activity was measured for Haplophyllum extracts. The present study brings forward the new data regarding the chemical profiles and antiradical activities of named Rutaceae species.

Acta Medica Medianae, Jan 15, 2022

The willow gentian (Gentiana asclepiadea L.) is a valuable source of secoiridoids, Cglycosylated ... more The willow gentian (Gentiana asclepiadea L.) is a valuable source of secoiridoids, Cglycosylated flavones and xanthones used empirically in the treatment of liver and gastrointestinal disorders. Guided by ethnopharmacological data on the use of G. asclepiadea underground parts in the treatment of diarrhea, antimicrobial activity against selected pathogens of gastrointestinal significance was examined. Presented study was aimed to evaluate antimicrobial activity of the aqueous-ethanolic extract of G. asclepiadea underground parts and its petroleum ether, ethyl acetate, butanol and water fractions. A molecular docking analysis was performed as well. The antimicrobial activity against pathogens related to gastrointestinal disorders was tested by a microdilution method. The ethyl acetate fraction showed the greatest antimicrobial activity. The lowest MIC of 0.78 mg/ml was observed against Bacillus cereus and Staphylococcus aureus, achieved by the petroleum ether and ethyl acetate fractions, respectively. The greatest bactericidal activity (MBC of 0.78 mg/ml), achieved by the ethyl acetate fractions, was recorded against Enterococcus faecalis. The yeast Candida albicans was the most resistant against the fractions and the extract. C-glycosylated flavones isoorientin and isovitexin showed the best binding affinity on Enterococcus faecalis lipoate-protein ligase A as determined by a molecular docking analysis. Considering the results of our study, underground parts of G. asclepiadea could be used as a valuable natural source of secondary metabolites with promising antimicrobial activity.

Central European Journal of Biology, Sep 1, 2009

Essential oil of Satureja hortensis L. was analyzed by GC and GC/MS and tested by a broth micro-w... more Essential oil of Satureja hortensis L. was analyzed by GC and GC/MS and tested by a broth micro-well dilution method for activity against multiresistant clinical isolates of pathogenic bacteria from 10 different genera: Klebsiella, Escherichia, Proteus, Staphylococcus, Streptococcus, Pseudomonas, Enterococcus, Enterobacter, Citrobacter and Acinetobacter. The main compounds in the oil were carvacrol (67%), γ-terpinene (15.3%) and p-cymene (6.73%). The oil showed activity against all tested strains. MIC/MBC values were in the range of 0.78-25 μl/ml, with the exception of the strain P. aeruginosa. Microbicidal concentration for this particular strain (50 μl/ml) was the highest tested concentration. The oil showed inhibitory and bactericidal effect at the same concentration (MIC=MBC) for all but three strains.

Health Care for Women International

The researchers' aims were to evaluate the effects of chokeberry juice in the treatment of bu... more The researchers' aims were to evaluate the effects of chokeberry juice in the treatment of burning mouth syndrome (BMS) and oral discomfort in menopausal women. The following validated scales and questionnaires were used before and after treatment: questionnaire used for selection of research participants with xerostomia, the xerostomia inventory, Visual Analogue Scale (VAS), localization of pain before and after treatment, short-form McGill pain questionnaire. There was statistically significant decrease in number of research participants who felt pain after therapeutic procedure (p < 0.05). Chokeberry juice, as additional therapy for BMS, reduced intensity of characteristic types of pain sensations and oral dryness feeling.

South African Journal of Botany

West Indian Medical Journal, 2015

We present a case of a 33-year old man who complained of weakness, fever and decreased urinating.... more We present a case of a 33-year old man who complained of weakness, fever and decreased urinating. A personal history revealed a consumption of creatine, protein supplements, soft drinks containing caffeine and stevia, and extreme physical activity which included lifting of heavy weights. The patient developed anuria, uraemia, fatigue, rhabdomyolysis and paradoxical hypokalaemia. After the patient had seven successive dialysis treatments, normal kidney function was restored. The report presents the first case of acute renal failure followed by hypokalaemia due to the combined action of the excessive consumption of supplements, soft drinks with stevia and caffeine, and extreme physical activity.

Acta Medica Medianae, 2017

Nowadays, the use of herbal dietary supplements in diabetic patients is becoming very popular, fo... more Nowadays, the use of herbal dietary supplements in diabetic patients is becoming very popular, following the opinion that the use of dietary supplements combined with conventional anti-diabetic drugs is absolutely safe and justified. The aim of this study was to investigate the potential role of healthcare professionals regarding the use of herbal dietary supplements with hypoglycemic effect in the population of patients with diabetes mellitus type 2. The research in the form of an analytical cross-sectional study was conducted in 6 pharmacies in the territory of Nis during October 2013. The study enrollment criterion was to include the patients diagnosed with type 2 diabetes who used pharmacotherapy in addition to herbal supplements. The surveyed women showed a statistically significant difference in the frequency of using herbal supplements compared to the group of men (P <0.001). There was a statistically significant difference between the age groups of respondents in relation to the influence of health service professionals and media on making decisions about the use of herbal dietary supplements. A multidisciplinary approach of healthcare professionals to pharmacotherapy of chronic disease is recommended. It is essential that doctors and pharmacists recognize the safety and efficacy issues prior to recommending herbal dietary supplements for diabetes care, because of the limited data available regarding the use of dietary supplements in diabetes.

Medical principles and practice : international journal of the Kuwait University, Health Science Centre, Jan 28, 2018

The purpose of this study was to investigate the relaxative effects of black currant juice on the... more The purpose of this study was to investigate the relaxative effects of black currant juice on the gastrointestinal smooth muscle in vitro. Berries of black currant cultivar Ometa were used for preparation of juice used in this study. Spasmolytic activity of black currant juice was tested on rat ileum isolated from male Wistar rats by monitoring its influence on spontaneous contractions, as well as contractions induced by potassium-chloride (KCl), barium-chloride (BaCl2), calcium-chloride (CaCl2) and acetylcholine (Ach). The results were expressed as mean ± standard deviation obtained in six measurements and statistical significance was determined by Students` t-test, with p<0.05 taken as significant. Black currant cultivar Ometa significantly reduced the frequency and the amplitude of spontaneous contractions (57.94 ± 3.44%) and Ach-induced contractions (42.74 ± 5.36%) (p < 0.05) of the isolated rat ileum. Cumulative concentrations (0.01-3 mg/ml) of the Ometa juice also reduce...

Acta medica medianae, 2015

Anethum graveolens L. is a member of the Apiaceae family and more commonly known as dill. Dill ha... more Anethum graveolens L. is a member of the Apiaceae family and more commonly known as dill. Dill has been used for gastrointestinal ailments such as flatulence, indigestion, stomach ache and colic. It has therapeutic effects such as mucosal protective, antisecretory, antimicrobial, antispasmodic, antihypercholesterolaemic and antihyperlipidaemic. The aim of our study was to examine the effects of the dill methanol extract on spontaneous and acetylcholine-induced contractions on isolated rat ileum. Segments of the rat ileum were suspended in an organ bath. The isolated ileum had been treated with the methanol extract of dill in cumulative concentrations (0.003-1mg/mL). In the second series of experiments, acetylcholine (5-1500nM) was cumulatively added to the bath in the absence and presence of methanol extract of dill (0.3-1mg/mL). Cumulative concentrations of methanol extract of dill significantly reduced the spontaneous rat ileum contractions (p<0.01) with EC50 value of 6.45 ±0.87mg/mL. The methanol dill extract concentration-dependently inhibited the contraction induced with acetylcholine (p<0.01), with an EC50 value of 0.41±0.057nM and 1.10±0.29nM (the EC50 value of acetylcholine was 0.06±0.0097nM). Our results showed the relaxant effect of the methanol dill extract on the isolated rat intestine. Extract of dill inhibited the spontaneous ileum contractions and contractions induced by acetylcholine.

Acta Facultatis Medicae Naissensis, 2015

Summary The aim of this study was to investigate whether green tea extract has beneficial effect ... more Summary The aim of this study was to investigate whether green tea extract has beneficial effect on gentamicin-induced acute renal failure. The investigation was conducted on thirty-two Wistar rats divided into four groups of 8 animals each. Control (C) group received normal saline. GT group received green tea extract orally, 300mg/kg. GM group received gentamicin intraperitoneally, 100mg/kg and GT+GM group received both gentamicin and green tea extract. Histological sections of kidney in GM group revealed necrosis of proximal tubules, vacuolization of cytoplasm and massive mononuclear inflammatory infiltrates in interstitium. Coadministration of green tea with gentamicin had renoprotective effect and showed only mild infiltrations, normal glomeruli and alleviated tubular degeneration. Analysis of biochemical parameters showed significantly higher urea and creatinine serum concentrations in GM group in comparison with C group and GT+GM group (p<0.001). Plasma lipid peroxidation b...

Acta Medica Medianae, 2020

Cereals are considered one of the most commonly used foods due to their properties. They are the ... more Cereals are considered one of the most commonly used foods due to their properties. They are the main source of carbohydrates, mainly starch. In addition to carbohydrates, cereals also contain proteins, unsaturated fatty acids, minerals, B-group vitamins and vitamin E. This composition refers to the unprocessed form of cereals-whole grain cereals. They also contain phenolic compounds, such as phenolic acid, flavonoids, stilbene, coumarins and tannins. These are plants secondary metabolites and have a significant role in the prevention of many diseases due to their antioxidant properties. The aim of this research was to determine the content of total polyphenols and tannins in wheat (type 500), maize, rye and whole wheat flour extracts, as well as the antioxidant activity of the samples. The highest content of total polyphenols was determined in whole wheat flour (6.38 ± 0.759 mg GAE/g), while the lowest amount was determined in wheat (type 500) flour (2.29 ± 0.32 mg GAE/g). Whole wheat flour extract showed the highest antioxidant activity (IC50 = 5.63 ± 1.331 mg/ml), while wheat flour (type 500) extract showed the lowest antioxidant activity (IC50 = 26.39 ± 2.132 mg/ml). Based on the results obtained, it can be concluded that whole grain cereals contain a large amount of polyphenols, which indicates a significant antioxidant activity. Due to their many properties, which have effect on improving health, cereals form the base of the pyramid of nutrition, as a model of healthy eating.

Pharmaceutics

The use of medicinal plant species and their products is widespread in the field of gastrointesti... more The use of medicinal plant species and their products is widespread in the field of gastrointestinal and respiratory diseases. This study aimed to evaluate the traditional use of Salvia sclarea L., clary sage, finding the possible mechanisms of its spasmolytic and bronchodilator actions in in vitro conditions supported by molecular docking analysis, along with the antimicrobial effects. Four dry extracts were prepared from the aerial parts of S. sclarea, using absolute or 80% (v/v) methanol by the method of a single-stage maceration or an ultrasound-assisted extraction. Characterization of the bioactive compounds by high-performance liquid chromatography indicated the presence of significant amounts of polyphenolics, with rosmarinic acid as the prevalent one. The spontaneous ileal contractions were best inhibited by the extract prepared with 80% methanol and maceration. The same extract was superior in the carbachol- and KCl-induced tracheal smooth muscle contractions, being the str...

Journal of Essential Oil Research, Mar 1, 2002

ABSTRACT An oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball obtained by hydr... more ABSTRACT An oil of Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P. W. Ball obtained by hydrodistillation was analyzed by GC and GC/MS and 36 constituents were identified (98.4%). The main constituents in the oil were pulegone (37.5%), menthone (17,6%), piperitenone (15.0%) and piperitone (10.2%).The antimicrobial activity of the oil was screened against Aspergillus niger, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Bacillus subtilis and Pseudomonas aeruginosa. The microorganisms were found to be susceptible to the oil

Lekovite sirovine, 2022

Birch leaves and bark are traditionally used for folk medicine for centuries. Numerous research f... more Birch leaves and bark are traditionally used for folk medicine for centuries. Numerous research focused on the chemical composition and biological effects of birch extracts. The main pharmacologically active compounds found in extracts are flavonoids, saponins, tannins, essential oil, and triterpenes. Different species, age of the plant, geographical region, extraction methods, and analytical techniques influence the chemical composition. Possible role of birch extracts in preventing and treating numerous diseases is enabled by their diuretic, antimicrobial, anti-inflammatory, and anti-allergic activity. Therefore, this article is intended to give a general overview of the studies on birch leaves and bark.

Acta Medica Medianae, Dec 15, 2016

Berries have become inevitable part of the diet of conscious men. Even though berries are not the... more Berries have become inevitable part of the diet of conscious men. Even though berries are not the richest source of zinc, there are agricultural practices that increase zinc content in surface soils. Being markedly involved in the human physiology and biochemistry, zinc is also a common component of plant metabolic pathways. A zinc defficiency occurs in plants mostly in calcareous and alkaline soils, affecting young leaves first. The toxicity and intolerence of zinc, which occur in the soils with prolonged use of fertilizers containing zinc among other elements, are greater problems than zinc deficiency. Zinc toxicity is often associated with the deficiency of magnesium, iron or manganese. In humans, zinc deficiency leads to acrodermatitis enteropathica, growth retardation, hypogonadism, depressed mental function, impaired cognitive functions and immune disorders, affecting males and females in the developing world. Serum zinc was correlated with the severity of depression and low serum zinc levels were found in depressed patients, suggesting that serum zinc could be the marker of depression. Diverse neurodegenerative processes, as Alzheimer's disease, may change the cellular zinc level, raising it to the level where zinc contributes to the progression of the disease. Zinc plays a pivotal role in few signal transduction and gene expression pathways, including that of cytokine genes. Zinc also holds a key position for multiple functions of cell metabolism, retinal development and specific retinal functions. Zinc in therapeutic dosages was effective in decreasing the incidence of infections. Zinc lozenges reduced the duration and severity of the common cold.

Acta Facultatis Medicae Naissensis, 2023

Lekovite sirovine, 2022

The term "phytopreparations" indicates products with active substances obtained from medicinal pl... more The term "phytopreparations" indicates products with active substances obtained from medicinal plants. Herbal medicines, extracts, essential oils, and isolated active principles are present in both cosmetology and dermatology. The new term, "cosmeceutical" refers to cosmetic preparations which also have therapeutic action. A review of relevant literature and legal frames has been done in order to understand the modern use of medicinal herbs in dermatological disorders. There is no single legal framework in this area. In the therapeutic field of skin disorders and minor wounds, there are 31 European Union monographs on herbal medicinal products prepared by the Committee on Herbal Medicinal Products, while the European Scientific Cooperative on Phytotherapy recommends the use of 19 herbal drugs for dermatological disorders, such as acne, dermatitis, eczema, wounds, skin inflammation, insect bites, etc. Most patients assume an herbal product is "safe" and without side effects simply because it's natural. The rational use of herbal cosmetic products requires appropriate knowledge of used plant materials and potential side effects.

South African Journal of Botany, Nov 1, 2022

Separation and Purification Technology, 2022

Central European Journal of Biology, Jul 1, 2014

The present study investigates the chemical compositions of three Satureja montana L. ssp montana... more The present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100-500-800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.

Biologica Nyssana, Dec 29, 2018

Family Rutaceae is presented in Serbian flora with 3 genera and 4 species: Ruta graveolens L., Di... more Family Rutaceae is presented in Serbian flora with 3 genera and 4 species: Ruta graveolens L., Dictamnus albus L., Haplophyllum suaveolens (DC.) G. Don and H. boissieranum Vis. & Pančić. Comparative study of the phytochemical variability and antioxidant activity of ethanolic and methanolic extracts, obtained from these species' areal parts, was performed. Total flavonoid content was determined spectrophotometrically and HPLC method was employed for quantification of coumarins. Fast antioxidant screening of samples was done in DPPH test. Flavonoid content of the tested samples varied from 2.82±0.27 to 27.72±0.15 mg of rutin equivalent (Ru)/g of extract. Psoralen, bergapten and xanthotoxin were detected and quantified only in R. graveolens extracts. All the tested samples exhibited notable potential in DPPH radicals scavenging assay. Yet, considerably lower activity was measured for Haplophyllum extracts. The present study brings forward the new data regarding the chemical profiles and antiradical activities of named Rutaceae species.

Acta Medica Medianae, Jan 15, 2022

The willow gentian (Gentiana asclepiadea L.) is a valuable source of secoiridoids, Cglycosylated ... more The willow gentian (Gentiana asclepiadea L.) is a valuable source of secoiridoids, Cglycosylated flavones and xanthones used empirically in the treatment of liver and gastrointestinal disorders. Guided by ethnopharmacological data on the use of G. asclepiadea underground parts in the treatment of diarrhea, antimicrobial activity against selected pathogens of gastrointestinal significance was examined. Presented study was aimed to evaluate antimicrobial activity of the aqueous-ethanolic extract of G. asclepiadea underground parts and its petroleum ether, ethyl acetate, butanol and water fractions. A molecular docking analysis was performed as well. The antimicrobial activity against pathogens related to gastrointestinal disorders was tested by a microdilution method. The ethyl acetate fraction showed the greatest antimicrobial activity. The lowest MIC of 0.78 mg/ml was observed against Bacillus cereus and Staphylococcus aureus, achieved by the petroleum ether and ethyl acetate fractions, respectively. The greatest bactericidal activity (MBC of 0.78 mg/ml), achieved by the ethyl acetate fractions, was recorded against Enterococcus faecalis. The yeast Candida albicans was the most resistant against the fractions and the extract. C-glycosylated flavones isoorientin and isovitexin showed the best binding affinity on Enterococcus faecalis lipoate-protein ligase A as determined by a molecular docking analysis. Considering the results of our study, underground parts of G. asclepiadea could be used as a valuable natural source of secondary metabolites with promising antimicrobial activity.

Central European Journal of Biology, Sep 1, 2009

Essential oil of Satureja hortensis L. was analyzed by GC and GC/MS and tested by a broth micro-w... more Essential oil of Satureja hortensis L. was analyzed by GC and GC/MS and tested by a broth micro-well dilution method for activity against multiresistant clinical isolates of pathogenic bacteria from 10 different genera: Klebsiella, Escherichia, Proteus, Staphylococcus, Streptococcus, Pseudomonas, Enterococcus, Enterobacter, Citrobacter and Acinetobacter. The main compounds in the oil were carvacrol (67%), γ-terpinene (15.3%) and p-cymene (6.73%). The oil showed activity against all tested strains. MIC/MBC values were in the range of 0.78-25 μl/ml, with the exception of the strain P. aeruginosa. Microbicidal concentration for this particular strain (50 μl/ml) was the highest tested concentration. The oil showed inhibitory and bactericidal effect at the same concentration (MIC=MBC) for all but three strains.

Health Care for Women International

The researchers' aims were to evaluate the effects of chokeberry juice in the treatment of bu... more The researchers' aims were to evaluate the effects of chokeberry juice in the treatment of burning mouth syndrome (BMS) and oral discomfort in menopausal women. The following validated scales and questionnaires were used before and after treatment: questionnaire used for selection of research participants with xerostomia, the xerostomia inventory, Visual Analogue Scale (VAS), localization of pain before and after treatment, short-form McGill pain questionnaire. There was statistically significant decrease in number of research participants who felt pain after therapeutic procedure (p < 0.05). Chokeberry juice, as additional therapy for BMS, reduced intensity of characteristic types of pain sensations and oral dryness feeling.

South African Journal of Botany

West Indian Medical Journal, 2015

We present a case of a 33-year old man who complained of weakness, fever and decreased urinating.... more We present a case of a 33-year old man who complained of weakness, fever and decreased urinating. A personal history revealed a consumption of creatine, protein supplements, soft drinks containing caffeine and stevia, and extreme physical activity which included lifting of heavy weights. The patient developed anuria, uraemia, fatigue, rhabdomyolysis and paradoxical hypokalaemia. After the patient had seven successive dialysis treatments, normal kidney function was restored. The report presents the first case of acute renal failure followed by hypokalaemia due to the combined action of the excessive consumption of supplements, soft drinks with stevia and caffeine, and extreme physical activity.

Acta Medica Medianae, 2017

Nowadays, the use of herbal dietary supplements in diabetic patients is becoming very popular, fo... more Nowadays, the use of herbal dietary supplements in diabetic patients is becoming very popular, following the opinion that the use of dietary supplements combined with conventional anti-diabetic drugs is absolutely safe and justified. The aim of this study was to investigate the potential role of healthcare professionals regarding the use of herbal dietary supplements with hypoglycemic effect in the population of patients with diabetes mellitus type 2. The research in the form of an analytical cross-sectional study was conducted in 6 pharmacies in the territory of Nis during October 2013. The study enrollment criterion was to include the patients diagnosed with type 2 diabetes who used pharmacotherapy in addition to herbal supplements. The surveyed women showed a statistically significant difference in the frequency of using herbal supplements compared to the group of men (P <0.001). There was a statistically significant difference between the age groups of respondents in relation to the influence of health service professionals and media on making decisions about the use of herbal dietary supplements. A multidisciplinary approach of healthcare professionals to pharmacotherapy of chronic disease is recommended. It is essential that doctors and pharmacists recognize the safety and efficacy issues prior to recommending herbal dietary supplements for diabetes care, because of the limited data available regarding the use of dietary supplements in diabetes.

Medical principles and practice : international journal of the Kuwait University, Health Science Centre, Jan 28, 2018

The purpose of this study was to investigate the relaxative effects of black currant juice on the... more The purpose of this study was to investigate the relaxative effects of black currant juice on the gastrointestinal smooth muscle in vitro. Berries of black currant cultivar Ometa were used for preparation of juice used in this study. Spasmolytic activity of black currant juice was tested on rat ileum isolated from male Wistar rats by monitoring its influence on spontaneous contractions, as well as contractions induced by potassium-chloride (KCl), barium-chloride (BaCl2), calcium-chloride (CaCl2) and acetylcholine (Ach). The results were expressed as mean ± standard deviation obtained in six measurements and statistical significance was determined by Students` t-test, with p<0.05 taken as significant. Black currant cultivar Ometa significantly reduced the frequency and the amplitude of spontaneous contractions (57.94 ± 3.44%) and Ach-induced contractions (42.74 ± 5.36%) (p < 0.05) of the isolated rat ileum. Cumulative concentrations (0.01-3 mg/ml) of the Ometa juice also reduce...

Acta medica medianae, 2015

Anethum graveolens L. is a member of the Apiaceae family and more commonly known as dill. Dill ha... more Anethum graveolens L. is a member of the Apiaceae family and more commonly known as dill. Dill has been used for gastrointestinal ailments such as flatulence, indigestion, stomach ache and colic. It has therapeutic effects such as mucosal protective, antisecretory, antimicrobial, antispasmodic, antihypercholesterolaemic and antihyperlipidaemic. The aim of our study was to examine the effects of the dill methanol extract on spontaneous and acetylcholine-induced contractions on isolated rat ileum. Segments of the rat ileum were suspended in an organ bath. The isolated ileum had been treated with the methanol extract of dill in cumulative concentrations (0.003-1mg/mL). In the second series of experiments, acetylcholine (5-1500nM) was cumulatively added to the bath in the absence and presence of methanol extract of dill (0.3-1mg/mL). Cumulative concentrations of methanol extract of dill significantly reduced the spontaneous rat ileum contractions (p<0.01) with EC50 value of 6.45 ±0.87mg/mL. The methanol dill extract concentration-dependently inhibited the contraction induced with acetylcholine (p<0.01), with an EC50 value of 0.41±0.057nM and 1.10±0.29nM (the EC50 value of acetylcholine was 0.06±0.0097nM). Our results showed the relaxant effect of the methanol dill extract on the isolated rat intestine. Extract of dill inhibited the spontaneous ileum contractions and contractions induced by acetylcholine.

Acta Facultatis Medicae Naissensis, 2015

Summary The aim of this study was to investigate whether green tea extract has beneficial effect ... more Summary The aim of this study was to investigate whether green tea extract has beneficial effect on gentamicin-induced acute renal failure. The investigation was conducted on thirty-two Wistar rats divided into four groups of 8 animals each. Control (C) group received normal saline. GT group received green tea extract orally, 300mg/kg. GM group received gentamicin intraperitoneally, 100mg/kg and GT+GM group received both gentamicin and green tea extract. Histological sections of kidney in GM group revealed necrosis of proximal tubules, vacuolization of cytoplasm and massive mononuclear inflammatory infiltrates in interstitium. Coadministration of green tea with gentamicin had renoprotective effect and showed only mild infiltrations, normal glomeruli and alleviated tubular degeneration. Analysis of biochemical parameters showed significantly higher urea and creatinine serum concentrations in GM group in comparison with C group and GT+GM group (p<0.001). Plasma lipid peroxidation b...

Acta Medica Medianae, 2020

Cereals are considered one of the most commonly used foods due to their properties. They are the ... more Cereals are considered one of the most commonly used foods due to their properties. They are the main source of carbohydrates, mainly starch. In addition to carbohydrates, cereals also contain proteins, unsaturated fatty acids, minerals, B-group vitamins and vitamin E. This composition refers to the unprocessed form of cereals-whole grain cereals. They also contain phenolic compounds, such as phenolic acid, flavonoids, stilbene, coumarins and tannins. These are plants secondary metabolites and have a significant role in the prevention of many diseases due to their antioxidant properties. The aim of this research was to determine the content of total polyphenols and tannins in wheat (type 500), maize, rye and whole wheat flour extracts, as well as the antioxidant activity of the samples. The highest content of total polyphenols was determined in whole wheat flour (6.38 ± 0.759 mg GAE/g), while the lowest amount was determined in wheat (type 500) flour (2.29 ± 0.32 mg GAE/g). Whole wheat flour extract showed the highest antioxidant activity (IC50 = 5.63 ± 1.331 mg/ml), while wheat flour (type 500) extract showed the lowest antioxidant activity (IC50 = 26.39 ± 2.132 mg/ml). Based on the results obtained, it can be concluded that whole grain cereals contain a large amount of polyphenols, which indicates a significant antioxidant activity. Due to their many properties, which have effect on improving health, cereals form the base of the pyramid of nutrition, as a model of healthy eating.

Pharmaceutics

The use of medicinal plant species and their products is widespread in the field of gastrointesti... more The use of medicinal plant species and their products is widespread in the field of gastrointestinal and respiratory diseases. This study aimed to evaluate the traditional use of Salvia sclarea L., clary sage, finding the possible mechanisms of its spasmolytic and bronchodilator actions in in vitro conditions supported by molecular docking analysis, along with the antimicrobial effects. Four dry extracts were prepared from the aerial parts of S. sclarea, using absolute or 80% (v/v) methanol by the method of a single-stage maceration or an ultrasound-assisted extraction. Characterization of the bioactive compounds by high-performance liquid chromatography indicated the presence of significant amounts of polyphenolics, with rosmarinic acid as the prevalent one. The spontaneous ileal contractions were best inhibited by the extract prepared with 80% methanol and maceration. The same extract was superior in the carbachol- and KCl-induced tracheal smooth muscle contractions, being the str...