Ergin Kariptaş - Academia.edu (original) (raw)

Papers by Ergin Kariptaş

Journal of Istanbul Faculty of Medicine / İstanbul Tıp Fakültesi Dergisi, 2022

Objective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the... more Objective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the effects of vaginal Lactobacillus on cervical cancer cells have rarely been reported. The goal of this investigation was to assess the anti-proliferative effect on cancer cell line HeLa (Human Cervical Carcinoma Cell) and potential probiotic properties of Lactobacillus plantarum L4 isolated from cervicovaginal flora of healthy women in Turkey. Materials and Methods: Molecular identification of the species was performed by 16S rDNA analysis. Probiotic properties of the L4 strain were investigated by conventional methods. Human Interleukin-10 (IL-10) and Tumor Necrosis Factor-alpha (TNF-alpha) ELISA kits were used in the evelation of the immune modulator effect of the L4 strain. The antiproliferative effect of the L4 strain on the HeLA cell line was performed using the XTT kit. Results: L. plantarum L4 strain exhibited strong probiotic properties. The L4 strain showed an anti-inflammatory effect on HeLa by reducing the production of TNF-α and increased IL-10 production. The greatest antiproliferative effect of L. plantarum L4 strain on HeLa cells was observed at the highest dose of the metabolite 0.0006 gr/ml with a death rate of 90-95% while the number of living cells was found to be between 5-10%. The strain showed no anticancer effect on human umbilical vein endothelial cells (HUVEC). Conclusion: L. plantarum L4 strain, with strong probiotic properties, can be considered a promising treatment candidate for HPV cancer due to its immunomodulatory effect and high antiproliferative effect, even in very small doses.

A total of 120 fresh water fish samples were evaluated for the presence of Aeromonas spp. A. hydr... more A total of 120 fresh water fish samples were evaluated for the presence of Aeromonas spp. A. hydrophila, A. caviae ,a ndA. veronii bv. sobria were isolated from the gills, intestines, livers, and skins of fish, and 78 isolated Aeromonas spp. strains were further examined for siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance. Siderophore production was demonstrated in almost all of the A. hydrophila and A. caviae strains except for 2 strains of A. veronii bv. sobria. The strains identified as A. hydrophila and A. veronii bv. sobria were the stronger producers of hemolysin, whereas the A. caviae strains were nonhemolytic. It was found that 92% of A. hydrophila and 91% of A. caviae strains hydrolyzed pyrazinamide in under 48 h, whereas 5% of A. veronii bv. sobria strains hydrolyzed pyrazinamide. Protease activity was found in 100% of isolates of A. hydrophila and A. veronii bv. sobria, but in 81% of A. caviae isolates. In addition, all Aeromonas ...

Yaşlı Sorunları Araştırma Dergisi, 2020

Introduction: This study was conducted to examine the knowledge levels and practices of older ind... more Introduction: This study was conducted to examine the knowledge levels and practices of older individuals about rational drug use. Materials and Method: This descriptive study was conducted on 382 older individuals. The data were collected with the Rational Drug Use Survey through face-to-face interview method. The data were evaluated through the chi-square test and descriptive analysis methods. Results: In the study, 72.5 % of the older individuals stated that they used drugs without a prescription of a doctor, 23.6 % stated that they increased/decreased their doses without consulting the doctor, 91.1 % stated that they complied with the drug hours recommended by the doctors, and 44.2 % stated that they quit medication before the date prescribed by the doctor. Conclusion: The most important problems of older individuals concerning the rational drug use were determined to be using drugs without the prescription of a doctor and quitting the medication before the date recommended by the doctor. In line with these results, informing and supporting older individuals about rational drug use will make significant contribution for the rational drug use.

Current opinion in urology, Jan 16, 2017

Prostate cancer (PCa) diagnostics are evolving rapidly. The quest to differentiate 'clinicall... more Prostate cancer (PCa) diagnostics are evolving rapidly. The quest to differentiate 'clinically significant' from 'clinically insignificant' disease has gathered momentum, leading to substantial change in traditional diagnostic approaches. Herein, we review the relevant information on currently available biomarkers and assess their ability to help physicians and patients in making a shared and personalized decision based on their individual risk of harbouring clinically significant disease. Serum, urine, tissue and imaging biomarkers have been evaluated to improve the identification of clinically significant disease, and this international effort has yielded promising, but not always consistent results. Changes in MRI technology have realized a quantum change, and this facility is now becoming more widely incorporated into diagnostic and disease risk-stratification protocols. However, standardization and further validation is required. Acceptance and widespread adopti...

Phase Transitions, 2016

ABSTRACT Single crystals of 4-methylbenzylammonium sulfate were grown in an aqueous solution at r... more ABSTRACT Single crystals of 4-methylbenzylammonium sulfate were grown in an aqueous solution at room temperature. The grown compound is characterized by spectroscopic, thermal, and dielectric studies and its structure was determined by single-crystal X-ray diffraction analysis. Its crystal structure is described as a three-dimensional network where the sulfate anions (HSO4−) are interconnected through H-bonds to form anionic layers between which the 4-methylbenzylammonium cations are located. The hydrogen bonding network connecting the different components is given. Hirshfeld surface analysis was performed to visualize, explore and quantify intermolecular interactions in the crystal lattice. This analysis revealed the presence of H…C/H…C, C…O/O…C intermolecular interactions and O…O, H…H short contacts in the crystal. X-ray, structural and electrical results are correlated. The kind of the observed conduction is protonic by translocation. Differential scanning calorimetry (DSC) shows that this material presents a reversible phase transition at 390 K, confirmed by the dielectric permittivity study.

Turkish Journal of Biology, Oct 15, 2001

Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomi... more Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomic study, were evaluated by gas chromotography and pyrolysis mass spectrometry. Group I Streptomyces sp. AK409 and group II Streptomyces sp. AK029 selected duplicated strains were clustered in their own groups. It was observed that the results of gas chromatography and pyrolysis mass spectrometry was in good congruance with the numerical taxonomic results. Therefore, it can be concluded that the different chemotaxonomic methods provide rapid and reproducible information for classifying biotechnologically significant Streptomyces strains.

International Current Pharmaceutical Journal, 2016

In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium io... more In this study, the effect of the incubation time, incubation temperature, pH, metal and sodium ions on the antibacterial activity of chitosan against food borne spoilage bacteria focused. Aeromonas hydrophila and Staphylococcus aureus, were used as two food borne bacteria. Acetic acid was used dissolving of chitosan. Results showed that chitosan solution at 5 mg/mL significantly inhibited the growth of A. hydrophila and S. aureus in presence of time, pH and metal ions (p <0.01). However, temperature did not significantly affect the antibacterial activity (4°C, 25°C and 37°C) (p >0.01). Chitosan activity increased with increasing incubation time. The influence of below pH 6 on the antibacterial activity of chitosan was significantly influenced (p<0.01). The addition of Ba +2 reduced chitosan activity against A. hydrophila while the addition of Ca +2 have increased chitosan activity against S. aureus. Sodium ions at a concentration of 25 mM reduced chitosan activity against S. aureus. These results show that chitosan can be used food industry to prevent the development of spoilage bacteria.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009

In this study, whole cell protein profiles of 20 local strains of Aeromonas hydrophila isolated f... more In this study, whole cell protein profiles of 20 local strains of Aeromonas hydrophila isolated from different foods in Turkey and one reference strain (ATCC 7966) were analyzed by SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis). It was observed that there was variability among the strains. Molecular weight of proteins were found to be between 21-116 kDa. The protein polypeptide bands from 37.8 to 101.4 kDa were common in both local strains and reference strain of A. hydrophila. The results of this study indicated that there is a genetic similarity between strains of A. hydrophila and reference strain (ATCC 7966). These protein patterns are likely to be beneficial to differentiate between the strains in epidemiological studies.

TURKISH JOURNAL OF BIOLOGY, 2014

In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from... more In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm 2 to 29 and 33 dyne/cm 2 , and the CMC of produced rhamnolipids were 115 and 130 mg/L, respectively. P. aeruginosa 78 and 99 strains emulsified benzene and n-hexane at the highest rates, and the surfaces of these strains were 73% and 65% and 62% and 72% more hydrophobic for benzene and toluene, respectively.

Turk J Biol, 2002

In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxo... more In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically wellestablished species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia.

Medical Mycology, 2013

species are the fourth leading cause of nosocomial bloodstream infections [2]. And although C. al... more species are the fourth leading cause of nosocomial bloodstream infections [2]. And although C. albicans remains the leading and the most widespread pathogenic yeast species causing a variety of infections, the incidence rates of non-C. albicans Candida infections has increased in recent years, including those with intrinsic or acquired resistance to azole antifungals [1-3]. More recently, infections caused by less common yeast species such as Pichia , Rhodotorula , Trichosporon , and Saccharomyces spp. [4-8] and other rarely encountered species have been reported [1,9,10]. Rapid identifi cation of yeast isolates from clinical samples is particularly important to initiate appropriate therapy given their variable antifungal susceptibility profi les. However, this task is complicated by the increasing number of emerging pathogenic fungal species that are not included

Electronic Journal of Biotechnology, 2010

Influences of phenol, α-naphtol and β-naphtol, which are toxic chemicals, on citric acid biosynth... more Influences of phenol, α-naphtol and β-naphtol, which are toxic chemicals, on citric acid biosynthesis and biomass in the artificial culture setting of Aspergillus niger using batch fermenter are examined in the most favorable fermentation conditions, and a model is proposed. Addition of certain concentrations of phenol, α-naphtol and β-naphtol to the culture increases the citric acid production. According to this model, maximum citric acid concentration is 48.3 g L-1 in a culture that does not contain any toxic chemicals, whereas the maximum concentrations obtained in cultures containing 25 mg L-1 phenol, 25 mg L-1 alpha-naphtol and 15 mg L-1 beta-naphtol are 62.5 g L-1 , 78.1 g L-1 and 86.0 g L-1 , respectively. Moreover, addition of toxic chemicals to the culture reduces fermentation time by 24 hrs.

Turk. J. Biol, 2001

Abstract: Batch grown cultures of Rhodococcus ruber from different growth phases and continuous g... more Abstract: Batch grown cultures of Rhodococcus ruber from different growth phases and continuous grown cultures from carbon, phosphate and magnesium limitation were analysed by pyrolyis mass spectrometry (PyMS). Batch grown cells showed significantly different pyrograms ...

Turk. J. Biol, 2011

Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromona... more Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromonas strains taken from red meat, raw chicken, minced meat, and fi sh samples. Each strain was tested for 86 characters but only the fi nal data including 63 characters were analysed using the S SM coeffi cients and the UPGMA clustering algorithm. At S SM values of ≥ 83%, the strains clustered into 10 aggregate groups consisting of 7 major (5 and up strains) and 3 minor (2-4 strains), and 5 single member clusters, each of which was identifi ed as A. hydrophila, A. caviae, and A. sobria, respectively. It was proved that the food isolates showed a relative phenotypical distance and the groups of strains that had atypical profi les were compared with the type species by the present identifi cation schemes. It was clearly seen that the phenetic approach was a necessary tool to delimitate and identify the Aeromonas species. Numerical taxonomy of Aeromonas strains isolated from diff erent sources revealed the presence of potentially pathogenic Aeromonas spp., especially in food.

Black Sea Journal of Health Science, 2021

Biyofilmler, 17. Yüzyılda Antonie van Leeuwenhoek'ın keşfiyle ve sonrasında birçok bilim insanını... more Biyofilmler, 17. Yüzyılda Antonie van Leeuwenhoek'ın keşfiyle ve sonrasında birçok bilim insanının çalışmalarıyla incelenmiş, gerek endüstri gerekse tıp alanındaki önemi son yıllarda ortaya konulmuştur. Bakteriyel mikroorganizmalar, biyofilm oluşumuyla kendilerini; antibakteriyel ajanlar, immün sistem elemanları ve çevresel tehditlere karşı savunurlar. Biyofilmler birçok hastalık oluşumuyla doğrudan ilişkilidir. Biyofilm içerisindeki mikroorganizmalar sinyal molekülleri aracılığıyla iletişim kurdukları "quorum sensing" denilen bir haberleşme sistemine sahiptirler. Bu mikroorganizmalar biyofilm üretmek yoluyla birçok avantaj elde ederler ve antimikrobiyal ajanlardan kendilerini korurlar. Günümüzde bakteriyel biyofilm oluşumu, Kongo kırmızılı agar yöntemi, Standart cam tüp yöntemi gibi farklı analiz yöntemleriyle incelenmektedir. Kolorimetrik yöntemler, mikroskobik yöntemler gibi farklı biyofilm kültür yöntemleri de mevcuttur.