EMANUEL Mitrea - Academia.edu (original) (raw)
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Tianjin University of Science and Technology
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Papers by EMANUEL Mitrea
AgroLife Scientific Journal, Dec 30, 2023
Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattl... more Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67-37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32-58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature.
Life
Canine babesiosis is an emerging tick-borne disease of major veterinary concern in Europe. Its pr... more Canine babesiosis is an emerging tick-borne disease of major veterinary concern in Europe. Its prevalence has increased in the last two decades and is spreading rapidly toward the north. The aim of this study was to investigate the genetic diversity of Babesia spp. strains isolated from naturally infected dogs in a tick-endemic area (Dobrogea) in southeastern Romania. For this purpose, a total of twenty-three samples from dogs diagnosed with various clinical forms of babesiosis, evaluated by means of clinical history, physical examination, and hematological tests, were subjected to a molecular investigation using PCR, sequencing analysis, and genetic characterization. A microscopic examination of thin Diff-quick-stained blood smears revealed large intra-erythrocytic Babesia piroplasms in all dogs. The PCR and sequencing analysis results indicated the presence of Babesia canis in 22 dogs (95.7%) and Babesia vogeli in 1 dog (4.3%). Among the B. canis isolates, two genotypes were disti...
AgroLife Scientific Journal, Dec 30, 2023
Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattl... more Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67-37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32-58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature.
Life
Canine babesiosis is an emerging tick-borne disease of major veterinary concern in Europe. Its pr... more Canine babesiosis is an emerging tick-borne disease of major veterinary concern in Europe. Its prevalence has increased in the last two decades and is spreading rapidly toward the north. The aim of this study was to investigate the genetic diversity of Babesia spp. strains isolated from naturally infected dogs in a tick-endemic area (Dobrogea) in southeastern Romania. For this purpose, a total of twenty-three samples from dogs diagnosed with various clinical forms of babesiosis, evaluated by means of clinical history, physical examination, and hematological tests, were subjected to a molecular investigation using PCR, sequencing analysis, and genetic characterization. A microscopic examination of thin Diff-quick-stained blood smears revealed large intra-erythrocytic Babesia piroplasms in all dogs. The PCR and sequencing analysis results indicated the presence of Babesia canis in 22 dogs (95.7%) and Babesia vogeli in 1 dog (4.3%). Among the B. canis isolates, two genotypes were disti...