Edith Hilario Torres Montoya - Academia.edu (original) (raw)

Papers by Edith Hilario Torres Montoya

Current Developments in Nutrition

Current Developments in Nutrition, 2021

Objectives To evaluate the effect of a high sucrose diet on the histopathological indicators rela... more Objectives To evaluate the effect of a high sucrose diet on the histopathological indicators related to NAFLD in male and female mice of the C57bl/6N strain. Methods Forty male and female mice of the C57bl/6N strain were randomly divided into four experimental groups: Control male (CMG) and female groups (CFG) with a standard diet (Rodent Chow) and tap water, as well as sucrose male (SMG) and female groups (SFG) which received standard diet and a 50% sucrose water solution. All the groups were fed ad libitum to one of the two different diets for 20 weeks. After 20 weeks, mice were sacrificed. Blood, hepatic and adipose tissues were collected for analyses. Hepatic tissues were sectioned and stained with hematoxylin and eosin. Samples were examined by light microscopy to analyze liver damage. The non-alcoholic steatohepatitis (NASH) degree was determined according to the histological score system proposed by the clinical research network of NASH. Results In hepatic histological sample...

Vector borne and zoonotic diseases, 2022

The transmission pathways of dengue virus (DENV) among mosquitoes are a topic that has gained rel... more The transmission pathways of dengue virus (DENV) among mosquitoes are a topic that has gained relevance in recent years because they could explain the maintenance of the virus in the wild independently of the human-mosquito horizontal transmission cycle. In this regard, Aedes aegypti larvae exposed to supernatants of C6/36 cells infected with DENV-4 were evaluated for virus excretion in feces and viability of infection in immature stages (larvae). The results demonstrate that larvae excrete DENV-4 in their feces with the potential to at least infect immature individuals of the same species. A horizontal transmission pathway of larvae-larvae DENV-4 under laboratory conditions is suggested.

Journal of helminthology, Jan 6, 2017

Gnathostoma turgidum is a nematode parasite that exploits the stomach of Virginian opossums, Dide... more Gnathostoma turgidum is a nematode parasite that exploits the stomach of Virginian opossums, Didelphis virginiana, in Latin America. The opossum is the definitive host of G. turgidum in the wild. Intrahepatic growth and maturation of the parasite, subsequent migration to the stomach and spontaneous expulsion are common. However, the histopathological lesions caused by G. turgidum are poorly described. A better understanding of the life cycle of this parasite and the pathological changes in natural host-parasite interactions could help to clarify the progression of human infections caused by Gnathostoma binucleatum. The aim of this work was to study morphological changes in the liver and stomach of D. virginiana during natural infection and adult worm expulsion. Three opossums naturally infected with G. turgidum were captured from an endemic area of gnathostomosis. Three uninfected opossums captured from a non-endemic area were used as controls. The opossums were sacrificed at differ...

Journal of the American Mosquito Control Association, 2015

We report here the discovery of Aedes albopictus for the first time in Sinaloa state, Mexico. The... more We report here the discovery of Aedes albopictus for the first time in Sinaloa state, Mexico. The mosquito larvae were collected from small water containers in the urban area of Culiacan city, Sinaloa state. Identification of the species was done primarily by morphology, followed by confirmation with polymerase-chain-reaction-based molecular method.

Journal of the American Mosquito Control Association, 2021

Mosquito larvae were collected in the urban area of the city of Culiacan, Sinaloa, in September o... more Mosquito larvae were collected in the urban area of the city of Culiacan, Sinaloa, in September of 2020. The immature stages were placed in emergence containers and fed with Aedes aegypti larvae. The adults that emerged from the immature stages were mounted on insect pins and characterized based on their morphology. The species corresponded morphologically to Toxorhynchites moctezuma, making this the first report of the species for the state of Sinaloa, Mexico. Similarities and morphological variations are discussed with previous analysis for this species.

Human gnathostomosis, a serious public health issue in Mexico, is endemic to Sinaloa. The disease... more Human gnathostomosis, a serious public health issue in Mexico, is endemic to Sinaloa. The disease is mainly caused by consumption of the raw meat of freshwater or estuarine fi shes infected with the advanced third stage larvae (AL3) of Gnathostoma binucleatum. In the present study, we examined estuarine turtles with a sample consisting of 23 Trachemys scripta and 5 Kinosternon integrum from Sinaloa, Mexico for the presence of Gnathostoma larvae; such examination was made by the pressing method of skeletal muscles between 2 glass plates. The results showed that both turtles harbored G. binucleatum AL3; identifi cation was achieved by morphology and also by PCR/sequencing of the ITS2 region of ribosomal DNA of the larvae. Infection prevalence was higher for K. integrum (80%) than for T. scripta (69.6%), but heavy infection (> 10 AL3/turtle) was observed in the larger sized individuals of T. scripta. Consumption of the raw meat of these turtles represents a risk to acquire the disease.

CienciaUAT, 2022

Los insectos asociados a productos almacenados dañan a una amplia variedad de alimentos en hogare... more Los insectos asociados a productos almacenados dañan a una amplia variedad de alimentos en hogares y expendios, provocando baja calidad nutricional e incluso riesgo a la salud de los consumidores. El objetivo de este estudio fue determinar las especies y abundancia de insectos asociados a alimentos secos almacenados en casas y tiendas de abarrotes de la ciudad de Culiacán, Sinaloa. Se obtuvieron productos alimenticios, en casas o tiendas de abarrotes, con indicios de daños por insectos o presencia de los mismos, los cuales fueron trasladados al laboratorio, donde se aislaron los imagos. Los organismos inmaduros y las muestras de alimento seco se depositaron en cámaras de emergencia de adultos debido a que la identificación se llevó a cabo por morfología en la fase de imago. Se recolectaron 181 ejemplares de insectos pertenecientes a 8 especies; de ellas, Oryzaephilus mercator (Fauvel), Necrobia rufipes (De Greer), Plodia interpunctella (Hubner) y Cadra cautella (Walker) son nuevos r...

Resumen. Los índices entomológicos son una herramienta habitual utilizada para la vigilancia de v... more Resumen. Los índices entomológicos son una herramienta habitual utilizada para la vigilancia de vectores con importancia en salud pública, como Aedes aegypti. En México y particularmente en Sinaloa estas prácticas entomológicas son utilizadas con frecuencia en áreas urbanas pero es incierta su aplicación en áreas rurales. El objetivo del estudio fue evaluar la fluctuación temporal del índice de positividad de ovitrampa (IPO), mediante la aplicación de un diseño modificado de ovitrampas, y el índice de casa (IC), en una zona rural de Sinaloa. Para este propósito, se determinó cada siete días la infestación de ovitrampas y casas en 20 viviendas habitadas en la comunidad de Rosa Morada, Navolato, Sinaloa, México, durante febrero a julio del 2016. No se observaron ovitrampas afectadas y la accesibilidad a las casas fue constante durante el tiempo del monitoreo. Ambos índices entomológicos aumentaron significativamente durante el periodo evaluado, con rangos que variaron de 0-60 y 15-40%...

Acta Parasitologica, 2021

PURPOSE Mexico is considered endemic for Leishmania; recent reports indicate autochthonous human ... more PURPOSE Mexico is considered endemic for Leishmania; recent reports indicate autochthonous human and canine leishmaniasis caused by Leishmania mexicana in Sinaloa state. Lutzomyia sand fly are the primary vector of the parasite, although no records of phlebotomine vectors of Leishmania exist from Sinaloa. Other hematophagous dipterans, like Culicoides, could represent possible vectors of Leishmania in absence of phlebotomines. The known distribution of Culicoides includes the southern portion of Sinaloa state, in northwestern Mexico, with records of Culicoides furens. However, no studies have demonstrated the presence of Leishmania in C. furens or its possible participation in the parasite's life cycle in Mexico. This study, therefore, sought to detect DNA of Leishmania in C. furens captured in an endemic area of autochthonous canine leishmaniasis in northwestern Mexico. METHODS Culicoides were captured with CDC light traps, identified morphologically, and organized in pools. DNA was extracted, and used to amplify the ribosomal ITS1 region of Leishmania. PCR products were digested with HaeIII endonuclease; the banding patterns obtained were compared to reference strains. RESULTS Leishmania mexicana DNA was detected in five out of nine pools (55%) of female C. furens. CONCLUSION This study offers the first evidence of L. mexicana DNA in C. furens, in an endemic area of canine leishmaniasis in northwestern Mexico, where no evidence exists of the presence of phlebotomine sand fly.

Sao Paulo Medical Journal, 2020

BACKGROUND: Diabetic nephropathy is a common complication of chronic kidney disease (CKD). Inflam... more BACKGROUND: Diabetic nephropathy is a common complication of chronic kidney disease (CKD). Inflammation in the kidneys is crucial for promoting development and progression of this complication. Wnt member 5a (Wnt5a) and secreted frizzled-related protein 5 (Sfrp5) are proinflammatory proteins associated with insulin resistance and chronic low-grade adipose tissue inflammation. OBJECTIVE: To determine the correlation between serum Sfrp5 and Wnt5a concentrations and glomerular filtration rate in patients with type 2 diabetes mellitus and CKD.

Southwestern Entomologist, 2018

Abstract. While infection by Gnathostoma turgidum (Stossich) in the definitive host, the Virginia... more Abstract. While infection by Gnathostoma turgidum (Stossich) in the definitive host, the Virginian opossum, Didelphis virginiana (Kerr), has been described in wildlife, the complete life cycle of the parasite is unknown. Development of early third-stage larvae of Gnathostoma spp. in vitro-infected cyclopoids has been evaluated previously. However, factors involved in viability of copepods as first-intermediate hosts for G. turgidum and that maintain the life cycle of the parasite in endemic areas of gnathostomosis have not been well studied. In this study, in vitro capability of Mesocyclops edax (Forbes) to ingest second-stage larvae of G. turgidum, and density-dependence relationship with the parasitic load were determined. Average ingestion of 1:5, 1:10, and 1:15 densities were 3.5 ± 0.3, 7.5 ± 0.6, and 10.8 ± 1.0, respectively; whereas parasitic loads of each density in M. edax after interaction were 1.7 ± 0.3, 2 ± 0.3, and 1.8 ± 0.3. Parasitic loads of copepods exposed to each density of second-stage larvae were not statistically different. Ingestion capacity by M. edax in vitro for larvae was directly proportional to density of larvae. However, the parasitic load tended to remain constant regardless of density of larvae to which the copepod was exposed. The behavior might be limited by the size of the host or co-evolutionary strategies that favor viability and maintenance of the life cycle of the parasite in wildlife.

Revista Mexicana de Biodiversidad, 2010

Acta Tropica, 2018

Leishmaniasis is a neglected tropical disease caused by different species of protozoan parasites ... more Leishmaniasis is a neglected tropical disease caused by different species of protozoan parasites of the genus Leishmania. Dogs have been proven as primary hosts of the parasite. Cases of cutaneous leishmaniasis in humans caused by Leishmania mexicana have been reported in Sinaloa; however, the vectors and hosts involved in the epidemiology of the parasite in northwestern Mexico are still unknown. Given the public health implications of this parasite's domestic hosts regarding the permanence and transmission of the disease to humans, the objective of the present study was to detect and determine the species of Leishmania that caused the first three cases of autochthonous canine leishmaniasis in the state of Sinaloa, Mexico. Three domestic dogs showing symptoms similar to canine leishmaniasis were identified, including chronic eye inflammation, corneal opacity, ocular exudate, emaciation and hyporexia. DNA was extracted from venous blood of the infected animals using a commercial kit. The internal transcribed spacer (ITS-1) of ribosomal DNA (rDNA) was amplified by specific primers for Leishmania from the extracted DNA, and the PCR products were digested with the restriction enzyme HaeIII. In addition, PCR products were subjected to automated sequencing. Molecular analysis showed that the infecting species was L. mexicana. This is the first report of autochthonous canine leishmaniasis caused by L. mexicana in Sinaloa, Mexico. Further studies are required to identify the species that serve as vectors and other wild and domestic hosts of the parasite, as well as to determine if there are more species of Leishmania circulating in Sinaloa.

Indian Journal of Medical Research, 2018

Background & objectives: Human papillomavirus (HPV) infections play a crucial role in the aet... more Background & objectives: Human papillomavirus (HPV) infections play a crucial role in the aetiology of cervical cancer (CC), and HPV16 is the primary viral genotype associated with CC. A number of variants of the HPV16 E6 gene are involved in the progression of CC, differing in their prevalence and biological and biochemical properties. This study was designed to determine the frequency of HPV types 16/18 and to identify the presence of HPV16 E6-variants in asymptomatic Mexican women. Methods: A total of 189 cervical Pap smears were collected from women attending public health services in three different cities in Sinaloa, Mexico. Viral DNA was identified by amplification of E6 viral gene fragments using polymerase chain reaction (PCR). Identification of variants was done by sequencing a DNA fragment (321bp) of the HPV16 E6 gene. Results: More than half of the women tested were HPV-positive (52.38%), with HPV16 being the most frequent genotype (21.16%), followed by HPV18 (8.99%). Sequence analysis of the E6-HPV16 PCR products showed that in all cases, the viruses corresponded to European variants. It was further observed that the E350G intra-variant was the most common (>76%). Interpretation & conclusions: This study showed a predominance of European lineage variants of HPV16 among asymptomatic women from Sinaloa, Mexico, predominantly with of the E350G variant. This variant has been shown to be associated with an increased risk of early development of CC. The use of molecular identification of carcinogenic HPV and Pap test screening may be a good strategy for monitoring women to prevent CC.

Vector borne and zoonotic diseases (Larchmont, N.Y.), Apr 1, 2018

Fourteen pools of Aedes aegypti larvae collected within the urban area of Culiacán, Sinaloa, were... more Fourteen pools of Aedes aegypti larvae collected within the urban area of Culiacán, Sinaloa, were analyzed by RT-PCR. The results demonstrate, for the first time, the vertical infection of serotype-2 dengue virus (DENV-2) in Sinaloa, Mexico, suggesting that Ae. aegypti acts as a natural reservoir of DENV-2 in this region.

Southwestern Entomologist, 2017

Revista Mexicana de Biodiversidad, 2016

La langosta de quelas rojas australiana (Cherax quadricarinatus) fue introducida a México hace un... more La langosta de quelas rojas australiana (Cherax quadricarinatus) fue introducida a México hace una década. Recientemente se han registrado poblaciones silvestres en distintas partes del país. Esta nota es el primer registro de C. quadricarinatus en medio silvestre en Sinaloa, México. Doce ejemplares adultos fueron capturados en distintos puntos del río Culiacán entre enero y marzo de 2015. Derechos Reservados © 2015 Universidad Nacional Autónoma de México, Instituto de Biología. Este es un artículo de acceso abierto distribuido bajo los términos de la Licencia Creative Commons CC BY-NC-ND 4.0.

The Southwestern Naturalist

ABSTRACT