Erika Hernandez - Academia.edu (original) (raw)

Papers by Erika Hernandez

Revista Facultad Nacional De Agronomia Medellin, Jun 1, 2012

In bovines, the transition period goes from the last three prepartum weeks to the first three pos... more In bovines, the transition period goes from the last three prepartum weeks to the first three postpartum weeks. This period is characterized by the metabolic adaptation of lipids, carbohydrates, minerals and proteins that try to balance the low intake of dry matter and the beginning of the secretion of milk. These changes are regulated by corticosteroids that are released at the time of birth. Some metabolites show noticeable changes in their blood concentration during this stage, which can lead to sanitary, reproductive and productive problems. The objective of the present work was to analyse the homeostasis in the transitional period, by determining the concentration of protein indicators, and some macrominerals, in dairy cows from two breeds under lowtropic conditions. The work was carried out in two commercial farms with similar management conditions in the Colombian south eastern region, in a agro-ecological zone of tropical dry forest according to , situated at 1,050 masl, with an average temperature of 23 ºC and an annual rainfall of 1200 mm bimodally distributed. The feed consisted of Bermuda grass (Cynodon nlemfuensis). The present work used seven Holstein cows and seven Hartón del Valle cows, from which blood samples were taken by coccygeal venepuncture in vacuum tubes, with heparin as anticoagulant and without anticoagulant, during the transition period, and up to the 60th day after delivery. A total of 156 samples were analysed. The following values were determined: total protein, albumin, globulin and urea; macro-minerals such as Ca, P and Mg and the cortisol hormone. The average values found for the Holstein breed were: total protein 66.7 g L -1 , albumin 37.6 g L -1 , globulins 27.7 g L -1 , urea 6.11 mmol L -1 , Ca 2.21 mmol L -1 , P 1.87 mmol L -1 , Mg 1.03 mmol L -1 and cortisol 33.2 nmol L -1 . For the Hartón del Valle breed the following average values were found: total protein 57.7 g L -1 , albumin 35.6 g L -1 , globulins 20.3 g L -1 , urea 5.37 mmol L -1 , Ca 2.56 mmol L -1 , P 1.81 mmol L -1 , Mg 0.81 mmol L -1 and cortisol 34.1 nmol L -1 . The only significant differences between the Hartón del Valle and the Holstein breeds were total protein, globulin and the serum cortisol levels.

Migracion Laboral Temporal Y Circular Experiencias Retos Y Oportunidades 2009 Pags 112 130, 2009

Acta Agronomica, 2011

(O SHULRGR GH SHULSDUWR R SHULRGR GH WUDQVLFLyQ SUHVHQWD YDULDFLRQHV ÀVLROyJLFDV VLJQLÀFDWLYDV qu... more (O SHULRGR GH SHULSDUWR R SHULRGR GH WUDQVLFLyQ SUHVHQWD YDULDFLRQHV ÀVLROyJLFDV VLJQLÀFDWLYDV que inciden en posteriores sucesos productivos (pico de lactancia, reactivación ovárica). El JDQDGR +DUWyQ SRVHH XQD H[FHOHQWH HÀFLHQFLD UHSURGXFWLYD HVWUHFKR LQWHUYDOR HQWUH SDUWRV IDFWRU TXH SRGUtD RULJLQDUVH HQ SURFHVRV PHWDEyOLFRV UHODFLRQDGRV FRQ XQ PHMRU DMXVWH KRPHRVWiWLFR (O SULQFLSDO REMHWLYR GHO SUHVHQWH WUDEDMR IXH HYDOXDU OD KRPHRVWDVLV HQ XQD UD]D ERYLQD FULROOD GXUDQWH HO SHULRGR SRVWSDUWR 3DUD HO HVWXGLR VH HPSOHDURQ YDFDV PXOWtSDUDV PXHVWUHDGDV DQWHV GHO SDUWR GtDV \ SUHSDUWR \ GLDULDPHQWH HQ ORV WUHV SULPHURV GtDV GHO SDUWR SRVWHULRUPHQWH D SDUWLU GHO GtD FRQ LQWHUYDORV GH FLQFR GtDV \ KDVWD HO GtD SRVWSDUWR (Q WRWDO SDUD FDGD DQLPDO VH DQDOL]DURQ PXHVWUDV FDGD XQD GH HOODV correspondió a un periodo analítico. Mediante venipunción coccígea en tubos al vacío con y sin anticoagulante se colectó la muestra de sangre completa. Por centrifugación se obtuvo SODVPD \ VXHUR ORV FXDOHV IXHURQ DOPDFHQDGRV D & KDVWD VX DQiOLVLV /DV GHWHUPLQDFLRQHV de hormonas se realizaron mediante radioinmunoanálisis (RIA) de fase sólida. A través de pruebas enzimáticas colorimétricas en equipos automatizados, se determinaron los valores GH ORV PHWDEROLWRV SDUD FDGD XQR GH ORV SHULRGRV GHÀQLGRV /RV YDORUHV PHGLRV IXHURQ %+% (E+LGUR[LEXWLUDWR PPRO/ 1()$ ÉFLGRV *UDVRV 1R (VWHULÀFDGRV PPRO/ WULJOLFpULGRV PPRO/ FROHVWHURO PPRO/ LQVXOLQD PUl/ml, triyodotironina (T3)

Acta Agronomica, Jan 8, 2014

1 conservación, mejoramiento y utilización del ganado criollo hartón del Valle y otros recursos g... more 1 conservación, mejoramiento y utilización del ganado criollo hartón del Valle y otros recursos genéticos animales en el suroccidente colombiano. Universidad Nacional de colombia sede Palmira. *correspondencia: eahernandez@unal.edu.co Palabras clave: Pennisetum clandestinum, adaptación, sistemas agropecuarios, cambio climático.

International Journal of Advanced Computer Science and Applications, 2015

In High Performance Computing, energy consumption is becoming an important aspect to consider. Du... more In High Performance Computing, energy consumption is becoming an important aspect to consider. Due to the high costs that represent energy production in all countries it holds an important role and it seek to find ways to save energy. It is reflected in some efforts to reduce the energy requirements of hardware components and applications. Some options have been appearing in order to scale down energy use and, consequently, scale up energy efficiency. One of these strategies is the multithread programming paradigm, whose purpose is to produce parallel programs able to use the full amount of computing resources available in a microprocessor. That energy saving strategy focuses on efficient use of multicore processors that are found in various computing devices, like mobile devices. Actually, as a growing trend, multicore processors are found as part of various specific purpose computers since 2003, from High Performance Computing servers to mobile devices. However, it is not clear how multiprogramming affects energy efficiency. This paper presents an analysis of different types of multicorebased architectures used in computing, and then a valid model is presented. Based on Amdahl's Law, a model that considers different scenarios of energy use in multicore architectures it is proposed. Some interesting results were found from experiments with the developed algorithm, that it was execute of a parallel and sequential way. A lower limit of energy consumption was found in a type of multicore architecture and this behavior was observed experimentally.

OTJR : occupation, participation and health, 2014

Researchers have reported positive associations between physical activity and academic achievemen... more Researchers have reported positive associations between physical activity and academic achievement. However, a common belief is that improving academic performance comes at the cost of reducing time for and resources spent on extracurricular activities that encourage physical activity. The purpose of this study was to examine the relationship between self-reported physical activity and grade point average (GPA) for health science graduate students. Graduate students in health science programs completed the International Physical Activity Questionnaire and reported their academic progress. Most participants (76%) reported moderate to vigorous physical activity levels that met or exceeded the recommended levels for adults. However, there was no significant correlation between GPA and level of physical activity. Negative findings for this study may be associated with the limited range of GPA scores for graduate students. Future studies need to consider more sensitive measures of cognit...

Journal of Health Communication, 2014

Entertainment education and the promotora model are 2 evidence-based health communication strateg... more Entertainment education and the promotora model are 2 evidence-based health communication strategies. This study examined their combined effect on promoting healthy eating among mothers in a family-based intervention. Participants were 361 Mexican-origin families living in Imperial County, California, who were randomly assigned to an intervention or delayed treatment condition. The intervention involved promotoras (community health workers) who delivered 11 home visits and 4 telephone calls. Home visits included a 12-minute episode of a 9-part situation comedy depicting a family struggling with making healthy eating choices; an accompanying family workbook was reviewed to build skills and left with the family. Baseline and immediate postintervention data were collected from the mothers, including the primary outcome of daily servings of fruits and vegetables. Other dietary and psychosocial factors related to healthy eating were examined. At postintervention, mothers in the intervention reported increases in daily vegetable servings (p .05); however, no changes were observed in fruit consumption. Improvements were observed in behavioral strategies to increase fiber (p .001) and to decrease fat intake (p .001), unhealthy eating behaviors (p .001), and individual (p .05) and family-related (p .01) perceived barriers to healthy eating. Entertainment education and promotoras engaged families and improved mothers' diets. Further research should examine the dose needed for greater changes.

Seminars in Cancer Biology, 2014

Given the multi-factorial nature of cancer, uncovering its metabolic alterations and evaluating t... more Given the multi-factorial nature of cancer, uncovering its metabolic alterations and evaluating their implications is a major challenge in biomedical sciences that will help in the optimal design of personalized treatments. The advance of high-throughput technologies opens an invaluable opportunity to monitor the activity at diverse biological levels and elucidate how cancer originates, evolves and responds under drug treatments. To this end, researchers are confronted with two fundamental questions: how to interpret high-throughput data and how this information can contribute to the development of personalized treatment in patients. A variety of schemes in systems biology have been suggested to characterize the phenotypic states associated with cancer by utilizing computational modeling and high-throughput data. These theoretical schemes are distinguished by the level of complexity of the biological mechanisms that they represent and by the computational approaches used to simulate them. Notably, these theoretical approaches have provided a proper framework to explore some distinctive metabolic mechanisms observed in cancer cells such as the Warburg effect. In this review, we focus on presenting a general view of some of these approaches whose application and integration will be crucial in the transition from local to global conclusions in cancer studies. We are convinced that multidisciplinary approaches are required to construct the bases of an integrative and personalized medicine, which has been and remains a fundamental task in the medicine of this century.

PLoS ONE, 2013

It has recently begun to be considered that cancer is a systemic disease and that it must be stud... more It has recently begun to be considered that cancer is a systemic disease and that it must be studied at every level of complexity using many of the currently available approaches, including high-throughput technologies and bioinformatics. To achieve such understanding in cervical cancer, we collected information on gene, protein and phosphoprotein expression of the HeLa cell line and performed a comprehensive analysis of the different signaling pathways, transcription networks and metabolic events in which they participate. A total expression analysis by RNA-Seq of the HeLa cell line showed that 19,974 genes were transcribed. Of these, 3,360 were over-expressed, and 2,129 under-expressed when compared to the NHEK cell line. A protein-protein interaction network was derived from the over-expressed genes and used to identify central elements and, together with the analysis of over-represented transcription factor motifs, to predict active signaling and regulatory pathways. This was further validated by Metal-Oxide Affinity Chromatography (MOAC) and Tandem Mass Spectrometry (MS/MS) assays which retrieved phosphorylated proteins. The 14-3-3 family members emerge as important regulators in carcinogenesis and as possible clinical targets. We observed that the different over-and underregulated pathways in cervical cancer could be interrelated through elements that participate in crosstalks, therefore belong to what we term ''meta-pathways''. Additionally, we highlighted the relations of each one of the differentially represented pathways to one or more of the ten hallmarks of cancer. These features could be maintained in many other types of cancer, regardless of mutations or genomic rearrangements, and favor their robustness, adaptations and the evasion of tissue control. Probably, this could explain why cancer cells are not eliminated by selective pressure and why therapy trials directed against molecular targets are not as effective as expected.

Journal of Public Health Management and Practice, 2007

All-hazards preparedness was evaluated in North Carolina&... more All-hazards preparedness was evaluated in North Carolina's 85 local health departments (LHDs). In regional meetings, data were collected from LHD teams from North Carolina's LHDs using an instrument constructed from Centers for Disease Control and Prevention's preparedness indicators and from the Local Public Health Preparedness and Response Capacity Inventory. Levels of preparedness differ widely by disaster types. LHDs reported higher levels of preparedness for natural disasters, outbreaks, and bioterrorist events than for chemical, radiation, or mass trauma disasters. LHDs face challenges to achieving all-hazards preparedness since preparation for one type of disaster does not lead to preparedness for all types of disasters. LHDs in this survey were more prepared for disasters for which they were funded (bioterrorism) and for events they faced regularly (natural disasters, outbreaks) than they were for other types of disasters.

Artificial Organs, 2012

This study evaluated the usefulness of the three-dimensional representation of electrocardiogram ... more This study evaluated the usefulness of the three-dimensional representation of electrocardiogram traces (3DECG) to reveal acute and gradual changes during a full session of hemodiafiltration (HDF) in end-stage renal disease (ESRD) patients. Fifteen ESRD patients were included (six men, nine women, age 46 ± 19 years old). Serum electrolytes, blood pressure, heart rate, and blood urea nitrogen (BUN) were measured before and after HDF. Continuous electrocardiograms (ECGs) obtained by Holter monitoring during HDF were used to produce the 3DECG. Several major disturbances were identified by 3DECG images: increase in QRS amplitude (47%), decrease in T-wave amplitude (33%), increase in heart rate (33%), and occurrence of arrhythmia (53%). Different arrhythmia types were often concurrent and included isolated supraventricular premature beats (N = 5), atrial fibrillation or atrial bigeminy (N = 2), and isolated premature ventricular beats (N = 6). Patients with decrease in T-wave amplitude had higher potassium and BUN (both before HDF and total removal) than those without decrease in T-wave amplitude (P < 0.05). Concurrent acute and gradual ECG changes during HDF are identified by the 3DECG, which could be useful as a preventive and prognostic method.

Ciência Animal …, 2009

CORTISOL E SUA RELAÇÃO COM A REGULAÇÃO ENDÓCRINA NO PERÍODO DE TRANSIÇÃO EM VACAS LEITEIRAS SOB C... more CORTISOL E SUA RELAÇÃO COM A REGULAÇÃO ENDÓCRINA NO PERÍODO DE TRANSIÇÃO EM VACAS LEITEIRAS SOB CONDIÇÕES DO TRÓPICO COLOMBIANO.

International Journal of Advanced Computer Science and Applications, 2014

The use of the Learning Management Systems (LMS) has been increased. It is desirable to access mu... more The use of the Learning Management Systems (LMS) has been increased. It is desirable to access multiple learning objects that are managed by Learning Management Systems. The diversity of LMS allow us to consider them as heterogeneous systems; each ones with their own interface to manage the provided functionality. These interfaces can be Web services or calls to remote objects. The functionalities offered by LMS depend on their user roles. A solution to integrate diverse heterogeneous platforms is based on a middleware architecture. In this paper, a middleware architecture is presented to integrate different Learning Management Systems. Furthermore, an implementation of the proposed middleware is presented. This implementation integrates two different Learning Management Systems, using Web services and XML-RPC protocols to access student-role users capabilities. The result is a transparent layer that provides access to LMS contents.

Revista Facultad Nacional De Agronomia Medellin, Jun 1, 2012

In bovines, the transition period goes from the last three prepartum weeks to the first three pos... more In bovines, the transition period goes from the last three prepartum weeks to the first three postpartum weeks. This period is characterized by the metabolic adaptation of lipids, carbohydrates, minerals and proteins that try to balance the low intake of dry matter and the beginning of the secretion of milk. These changes are regulated by corticosteroids that are released at the time of birth. Some metabolites show noticeable changes in their blood concentration during this stage, which can lead to sanitary, reproductive and productive problems. The objective of the present work was to analyse the homeostasis in the transitional period, by determining the concentration of protein indicators, and some macrominerals, in dairy cows from two breeds under lowtropic conditions. The work was carried out in two commercial farms with similar management conditions in the Colombian south eastern region, in a agro-ecological zone of tropical dry forest according to , situated at 1,050 masl, with an average temperature of 23 ºC and an annual rainfall of 1200 mm bimodally distributed. The feed consisted of Bermuda grass (Cynodon nlemfuensis). The present work used seven Holstein cows and seven Hartón del Valle cows, from which blood samples were taken by coccygeal venepuncture in vacuum tubes, with heparin as anticoagulant and without anticoagulant, during the transition period, and up to the 60th day after delivery. A total of 156 samples were analysed. The following values were determined: total protein, albumin, globulin and urea; macro-minerals such as Ca, P and Mg and the cortisol hormone. The average values found for the Holstein breed were: total protein 66.7 g L -1 , albumin 37.6 g L -1 , globulins 27.7 g L -1 , urea 6.11 mmol L -1 , Ca 2.21 mmol L -1 , P 1.87 mmol L -1 , Mg 1.03 mmol L -1 and cortisol 33.2 nmol L -1 . For the Hartón del Valle breed the following average values were found: total protein 57.7 g L -1 , albumin 35.6 g L -1 , globulins 20.3 g L -1 , urea 5.37 mmol L -1 , Ca 2.56 mmol L -1 , P 1.81 mmol L -1 , Mg 0.81 mmol L -1 and cortisol 34.1 nmol L -1 . The only significant differences between the Hartón del Valle and the Holstein breeds were total protein, globulin and the serum cortisol levels.

Migracion Laboral Temporal Y Circular Experiencias Retos Y Oportunidades 2009 Pags 112 130, 2009

Acta Agronomica, 2011

(O SHULRGR GH SHULSDUWR R SHULRGR GH WUDQVLFLyQ SUHVHQWD YDULDFLRQHV ÀVLROyJLFDV VLJQLÀFDWLYDV qu... more (O SHULRGR GH SHULSDUWR R SHULRGR GH WUDQVLFLyQ SUHVHQWD YDULDFLRQHV ÀVLROyJLFDV VLJQLÀFDWLYDV que inciden en posteriores sucesos productivos (pico de lactancia, reactivación ovárica). El JDQDGR +DUWyQ SRVHH XQD H[FHOHQWH HÀFLHQFLD UHSURGXFWLYD HVWUHFKR LQWHUYDOR HQWUH SDUWRV IDFWRU TXH SRGUtD RULJLQDUVH HQ SURFHVRV PHWDEyOLFRV UHODFLRQDGRV FRQ XQ PHMRU DMXVWH KRPHRVWiWLFR (O SULQFLSDO REMHWLYR GHO SUHVHQWH WUDEDMR IXH HYDOXDU OD KRPHRVWDVLV HQ XQD UD]D ERYLQD FULROOD GXUDQWH HO SHULRGR SRVWSDUWR 3DUD HO HVWXGLR VH HPSOHDURQ YDFDV PXOWtSDUDV PXHVWUHDGDV DQWHV GHO SDUWR GtDV \ SUHSDUWR \ GLDULDPHQWH HQ ORV WUHV SULPHURV GtDV GHO SDUWR SRVWHULRUPHQWH D SDUWLU GHO GtD FRQ LQWHUYDORV GH FLQFR GtDV \ KDVWD HO GtD SRVWSDUWR (Q WRWDO SDUD FDGD DQLPDO VH DQDOL]DURQ PXHVWUDV FDGD XQD GH HOODV correspondió a un periodo analítico. Mediante venipunción coccígea en tubos al vacío con y sin anticoagulante se colectó la muestra de sangre completa. Por centrifugación se obtuvo SODVPD \ VXHUR ORV FXDOHV IXHURQ DOPDFHQDGRV D & KDVWD VX DQiOLVLV /DV GHWHUPLQDFLRQHV de hormonas se realizaron mediante radioinmunoanálisis (RIA) de fase sólida. A través de pruebas enzimáticas colorimétricas en equipos automatizados, se determinaron los valores GH ORV PHWDEROLWRV SDUD FDGD XQR GH ORV SHULRGRV GHÀQLGRV /RV YDORUHV PHGLRV IXHURQ %+% (E+LGUR[LEXWLUDWR PPRO/ 1()$ ÉFLGRV *UDVRV 1R (VWHULÀFDGRV PPRO/ WULJOLFpULGRV PPRO/ FROHVWHURO PPRO/ LQVXOLQD PUl/ml, triyodotironina (T3)

Acta Agronomica, Jan 8, 2014

1 conservación, mejoramiento y utilización del ganado criollo hartón del Valle y otros recursos g... more 1 conservación, mejoramiento y utilización del ganado criollo hartón del Valle y otros recursos genéticos animales en el suroccidente colombiano. Universidad Nacional de colombia sede Palmira. *correspondencia: eahernandez@unal.edu.co Palabras clave: Pennisetum clandestinum, adaptación, sistemas agropecuarios, cambio climático.

International Journal of Advanced Computer Science and Applications, 2015

In High Performance Computing, energy consumption is becoming an important aspect to consider. Du... more In High Performance Computing, energy consumption is becoming an important aspect to consider. Due to the high costs that represent energy production in all countries it holds an important role and it seek to find ways to save energy. It is reflected in some efforts to reduce the energy requirements of hardware components and applications. Some options have been appearing in order to scale down energy use and, consequently, scale up energy efficiency. One of these strategies is the multithread programming paradigm, whose purpose is to produce parallel programs able to use the full amount of computing resources available in a microprocessor. That energy saving strategy focuses on efficient use of multicore processors that are found in various computing devices, like mobile devices. Actually, as a growing trend, multicore processors are found as part of various specific purpose computers since 2003, from High Performance Computing servers to mobile devices. However, it is not clear how multiprogramming affects energy efficiency. This paper presents an analysis of different types of multicorebased architectures used in computing, and then a valid model is presented. Based on Amdahl's Law, a model that considers different scenarios of energy use in multicore architectures it is proposed. Some interesting results were found from experiments with the developed algorithm, that it was execute of a parallel and sequential way. A lower limit of energy consumption was found in a type of multicore architecture and this behavior was observed experimentally.

OTJR : occupation, participation and health, 2014

Researchers have reported positive associations between physical activity and academic achievemen... more Researchers have reported positive associations between physical activity and academic achievement. However, a common belief is that improving academic performance comes at the cost of reducing time for and resources spent on extracurricular activities that encourage physical activity. The purpose of this study was to examine the relationship between self-reported physical activity and grade point average (GPA) for health science graduate students. Graduate students in health science programs completed the International Physical Activity Questionnaire and reported their academic progress. Most participants (76%) reported moderate to vigorous physical activity levels that met or exceeded the recommended levels for adults. However, there was no significant correlation between GPA and level of physical activity. Negative findings for this study may be associated with the limited range of GPA scores for graduate students. Future studies need to consider more sensitive measures of cognit...

Journal of Health Communication, 2014

Entertainment education and the promotora model are 2 evidence-based health communication strateg... more Entertainment education and the promotora model are 2 evidence-based health communication strategies. This study examined their combined effect on promoting healthy eating among mothers in a family-based intervention. Participants were 361 Mexican-origin families living in Imperial County, California, who were randomly assigned to an intervention or delayed treatment condition. The intervention involved promotoras (community health workers) who delivered 11 home visits and 4 telephone calls. Home visits included a 12-minute episode of a 9-part situation comedy depicting a family struggling with making healthy eating choices; an accompanying family workbook was reviewed to build skills and left with the family. Baseline and immediate postintervention data were collected from the mothers, including the primary outcome of daily servings of fruits and vegetables. Other dietary and psychosocial factors related to healthy eating were examined. At postintervention, mothers in the intervention reported increases in daily vegetable servings (p .05); however, no changes were observed in fruit consumption. Improvements were observed in behavioral strategies to increase fiber (p .001) and to decrease fat intake (p .001), unhealthy eating behaviors (p .001), and individual (p .05) and family-related (p .01) perceived barriers to healthy eating. Entertainment education and promotoras engaged families and improved mothers' diets. Further research should examine the dose needed for greater changes.

Seminars in Cancer Biology, 2014

Given the multi-factorial nature of cancer, uncovering its metabolic alterations and evaluating t... more Given the multi-factorial nature of cancer, uncovering its metabolic alterations and evaluating their implications is a major challenge in biomedical sciences that will help in the optimal design of personalized treatments. The advance of high-throughput technologies opens an invaluable opportunity to monitor the activity at diverse biological levels and elucidate how cancer originates, evolves and responds under drug treatments. To this end, researchers are confronted with two fundamental questions: how to interpret high-throughput data and how this information can contribute to the development of personalized treatment in patients. A variety of schemes in systems biology have been suggested to characterize the phenotypic states associated with cancer by utilizing computational modeling and high-throughput data. These theoretical schemes are distinguished by the level of complexity of the biological mechanisms that they represent and by the computational approaches used to simulate them. Notably, these theoretical approaches have provided a proper framework to explore some distinctive metabolic mechanisms observed in cancer cells such as the Warburg effect. In this review, we focus on presenting a general view of some of these approaches whose application and integration will be crucial in the transition from local to global conclusions in cancer studies. We are convinced that multidisciplinary approaches are required to construct the bases of an integrative and personalized medicine, which has been and remains a fundamental task in the medicine of this century.

PLoS ONE, 2013

It has recently begun to be considered that cancer is a systemic disease and that it must be stud... more It has recently begun to be considered that cancer is a systemic disease and that it must be studied at every level of complexity using many of the currently available approaches, including high-throughput technologies and bioinformatics. To achieve such understanding in cervical cancer, we collected information on gene, protein and phosphoprotein expression of the HeLa cell line and performed a comprehensive analysis of the different signaling pathways, transcription networks and metabolic events in which they participate. A total expression analysis by RNA-Seq of the HeLa cell line showed that 19,974 genes were transcribed. Of these, 3,360 were over-expressed, and 2,129 under-expressed when compared to the NHEK cell line. A protein-protein interaction network was derived from the over-expressed genes and used to identify central elements and, together with the analysis of over-represented transcription factor motifs, to predict active signaling and regulatory pathways. This was further validated by Metal-Oxide Affinity Chromatography (MOAC) and Tandem Mass Spectrometry (MS/MS) assays which retrieved phosphorylated proteins. The 14-3-3 family members emerge as important regulators in carcinogenesis and as possible clinical targets. We observed that the different over-and underregulated pathways in cervical cancer could be interrelated through elements that participate in crosstalks, therefore belong to what we term ''meta-pathways''. Additionally, we highlighted the relations of each one of the differentially represented pathways to one or more of the ten hallmarks of cancer. These features could be maintained in many other types of cancer, regardless of mutations or genomic rearrangements, and favor their robustness, adaptations and the evasion of tissue control. Probably, this could explain why cancer cells are not eliminated by selective pressure and why therapy trials directed against molecular targets are not as effective as expected.

Journal of Public Health Management and Practice, 2007

All-hazards preparedness was evaluated in North Carolina&... more All-hazards preparedness was evaluated in North Carolina's 85 local health departments (LHDs). In regional meetings, data were collected from LHD teams from North Carolina's LHDs using an instrument constructed from Centers for Disease Control and Prevention's preparedness indicators and from the Local Public Health Preparedness and Response Capacity Inventory. Levels of preparedness differ widely by disaster types. LHDs reported higher levels of preparedness for natural disasters, outbreaks, and bioterrorist events than for chemical, radiation, or mass trauma disasters. LHDs face challenges to achieving all-hazards preparedness since preparation for one type of disaster does not lead to preparedness for all types of disasters. LHDs in this survey were more prepared for disasters for which they were funded (bioterrorism) and for events they faced regularly (natural disasters, outbreaks) than they were for other types of disasters.

Artificial Organs, 2012

This study evaluated the usefulness of the three-dimensional representation of electrocardiogram ... more This study evaluated the usefulness of the three-dimensional representation of electrocardiogram traces (3DECG) to reveal acute and gradual changes during a full session of hemodiafiltration (HDF) in end-stage renal disease (ESRD) patients. Fifteen ESRD patients were included (six men, nine women, age 46 ± 19 years old). Serum electrolytes, blood pressure, heart rate, and blood urea nitrogen (BUN) were measured before and after HDF. Continuous electrocardiograms (ECGs) obtained by Holter monitoring during HDF were used to produce the 3DECG. Several major disturbances were identified by 3DECG images: increase in QRS amplitude (47%), decrease in T-wave amplitude (33%), increase in heart rate (33%), and occurrence of arrhythmia (53%). Different arrhythmia types were often concurrent and included isolated supraventricular premature beats (N = 5), atrial fibrillation or atrial bigeminy (N = 2), and isolated premature ventricular beats (N = 6). Patients with decrease in T-wave amplitude had higher potassium and BUN (both before HDF and total removal) than those without decrease in T-wave amplitude (P < 0.05). Concurrent acute and gradual ECG changes during HDF are identified by the 3DECG, which could be useful as a preventive and prognostic method.

Ciência Animal …, 2009

CORTISOL E SUA RELAÇÃO COM A REGULAÇÃO ENDÓCRINA NO PERÍODO DE TRANSIÇÃO EM VACAS LEITEIRAS SOB C... more CORTISOL E SUA RELAÇÃO COM A REGULAÇÃO ENDÓCRINA NO PERÍODO DE TRANSIÇÃO EM VACAS LEITEIRAS SOB CONDIÇÕES DO TRÓPICO COLOMBIANO.

International Journal of Advanced Computer Science and Applications, 2014

The use of the Learning Management Systems (LMS) has been increased. It is desirable to access mu... more The use of the Learning Management Systems (LMS) has been increased. It is desirable to access multiple learning objects that are managed by Learning Management Systems. The diversity of LMS allow us to consider them as heterogeneous systems; each ones with their own interface to manage the provided functionality. These interfaces can be Web services or calls to remote objects. The functionalities offered by LMS depend on their user roles. A solution to integrate diverse heterogeneous platforms is based on a middleware architecture. In this paper, a middleware architecture is presented to integrate different Learning Management Systems. Furthermore, an implementation of the proposed middleware is presented. This implementation integrates two different Learning Management Systems, using Web services and XML-RPC protocols to access student-role users capabilities. The result is a transparent layer that provides access to LMS contents.