Erlin Listiyaningsih - Academia.edu (original) (raw)

Papers by Erlin Listiyaningsih

ABSTRACT The elderly are part of the natural stage of life as human beings. Some people will make... more ABSTRACT The elderly are part of the natural stage of life as human beings. Some people will make various efforts to avoid the aging process. However, these efforts should be conducted as early as possible and cover the various factors involved in the aging process. A person in the aging process experiences various changes both physically and mentally, including a decline in both physical and physiological abilities. This community service activity is in the form of providing education about how to deal with their physical and physiological changes to get qualified and happy life, as well as simple health services. The implementation of the program takes place through the through the Community Partnership in "Improving the Quality of Life, for the Elderly through Family Assistance". The expected outcomes of this program are increased understanding of the occurrence of changes in the ability of physical activity and physiology in the elderly body, preparing for old age, and providing psychosocial support to families to achieve optimal independence in old age. This Partnership Program was implemented on Friday, April 19, 2019, at DKM Al Barokah, PWI Jaya Complex, Cilebut Bogor, and on Tuesday, April 23, 2019, at DKM Nurul Jihad Jl. North Jakarta Kalibaru Port. The total number of participants reached 78 people at the two community service locations along with DKM administrators, community leaders, and local religious leaders. In both populations, hypertension was the disease with the highest prevalence (37.2%), 12,8% had high blood glucose, 14% have dyslipidemia, and most (73.8%) of them did not perform routine physical activity. Keywords: Elderly, Quality Life, Family Support

Bioeduscience, Dec 31, 2022

Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging fro... more Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging from asymptomatic, mild symptoms such as flu-like illness and pneumonia to acute respiratory distress syndrome, which ends in death. Until now, the mechanism of the COVID-19 disease that causes widespread symptoms and the severity and factors that influence it are still unclear. During viral internalization, it needs to be cleaved by the serine protease encoded by the TMPRSS2 gene. It is hypothesized that higher expression of the TMPRSS2 gene causes higher virus internalization into cells, leading to more severe symptoms in patients. Methods: The Single Nucleotide Polymorphism Genotype Test was carried out to prove whether the TMPRSS2 gene affects the severity of COVID-19, as evidenced in other viral respiratory diseases. With a better understanding of gene expression related to this disease, it is hoped that we can better understand the mechanism of COVID-19 and establish better therapies and prevention against it. In this study, 68 COVID-19 patients participated and were categorized into two groups based on their clinical symptoms, namely mild symptoms without symptoms (n=12) and Moderate-Severe symptoms (n=56). PBMC cells were isolated from the patient. Then the DNA was extracted and used as a template in the SNP Genotyping of the TMPRSS2 rs2070788 gene variant. Results: The results showed that 35 samples had A/A homozygous genotypes, 29 A/G heterozygous samples, and 4 G/G homozygous samples. n=29) and heterozygous A/G (n=23), whereas only 4 were homozygous G/G. In addition, the homozygous G/G genotype was only detected in the moderate-severe group. Conclusions: A more significant number of samples from the asymptomatic mild symptom group is needed to statistically prove that homozygous G/G variants or G alleles are generally associated with the severity of COVID-19 patients.

Biota: jurnal ilmiah ilmu-ilmu hayati, Jul 16, 2021

Angina pectoris is one of the early symptoms that can be a marker of severe cardiovascular proble... more Angina pectoris is one of the early symptoms that can be a marker of severe cardiovascular problems such as coronary heart disease. While the PLA2G10 gene is believed to have a role, either in an atherosclerotic progression that causes plaque deposits and blockage in coronary arteries or on the contrary, which is antiatherogenic. PLA2G10 polymorphisms at SNP points T512C (rs36072688) and T-123/in1C (rs4003232) were able to change the expression level of the PLA2G10 gene as the gene encoding the sPLA 2-GX enzyme. This study aims to determine the genotype of PLA2G10 at SNP points T512C (rs36072688) and T-123/in1C (rs4003232) in angina pectoris patients and study the disease is caused by mutations or not. The research sample was stored biological material in the form of peripheral blood mononuclear cell collection of the Research and Development Division of Harapan Kita Cardiovascular Hospital. A total of 113 samples came from patients with angina pectoris with and without plaque in the coronary arteries. The results of this study indicate that the PLA2G10 gene at the SNP point T512C (rs36072688) has a wild-type homozygous genotype (TT) and T-123/In1C (rs4003232) has a heterozygous genotype (TC) through the TaqMan® SNP Genotyping Assay method, and no polymorphisms were found in the two sample groups.

International Journal of Angiology, Feb 18, 2021

Major adverse cardio-cerebrovascular events (MACCE) in ST-segment elevation myocardial infarction... more Major adverse cardio-cerebrovascular events (MACCE) in ST-segment elevation myocardial infarction (STEMI) are still high, although there have been advances in pharmacology and interventional procedures. Proprotein convertase subtilisin/Kexin type 9 ( PCSK9 ) is a serine protease regulating lipid metabolism associated with inflammation in acute coronary syndrome. The MACCE is possibly related to polymorphisms in PCSK9 . A prospective cohort observational study was designed to confirm the association between polymorphism of E670G and R46L in the PCSK9 gene with MACCE in STEMI. The Cox proportional hazards model and Spearman correlation were utilized in the study. The Genotyping of PCSK9 and ELISA was assayed. Sixty-five of 423 STEMI patients experienced MACCE in 6 months. The E670G polymorphism in PCSK9 was associated with MACCE (hazard ratio = 45.40; 95% confidence interval: 5.30–390.30; p = 0.00). There was a significant difference of PCSK9 plasma levels in patients with previous statin consumption (310 [220–1,220] pg/mL) versus those free of any statins (280 [190–1,520] pg/mL) ( p = 0.001). E670G polymorphism of PCSK9 was associated with MACCE in STEMI within a 6-month follow-up. The plasma PCSK9 level was higher in statin users.

PubMed, Jun 1, 2004

A study of epidemic transmission of Chikungunya virus (CHIK) was initiated in April 1999 in Yogya... more A study of epidemic transmission of Chikungunya virus (CHIK) was initiated in April 1999 in Yogyakarta, Indonesia. Three hundred seventeen volunteers from three kelurahans (sub-districts) were recruited. Anti-CHIK IgG antibodies were detected in 68% to 74% of cases and 28% to 32% of controls. In the kelurahan with no reported CHIK illness, 29% of cases and 28% of controls had anti-CHIK IgG antibodies. None of these cases demonstrated anti-CHIK IgM antibodies. In the two kelurahans with disease activity, anti-CHIK IgM antibodies were detected in 3% to 36% of cases, with the highest percentage from the kelurahan with recently reported cases. Ten percent of controls from Gowok had anti-CHIK IgM detected in their serum. Twelve acutely ill volunteers were later included from the kelurahan Pilahan for virus identification. Samples from two volunteers were culture- and RT-PCR-positive for CHIK. This is the first documentation of epidemic transmission of CHIK in Indonesia since 1982.

Journal of Medical Entomology, Sep 1, 2001

ABSTRACT

Emerging Infectious Diseases, 2010

Vascular Health and Risk Management, 2023

Background: Stroke is one of the highest causes of disability and mortality in several countries ... more Background: Stroke is one of the highest causes of disability and mortality in several countries worldwide. Secondary prevention is important in the management of stroke. Clopidogrel is widely used in Asia as secondary prevention for ischemic stroke, even though several studies in Western show limited data related to clopidogrel resistance in Asia. This study aims to determine the correlation between P2Y12 genetic polymorphism and clopidogrel resistance in Indonesia. Methods: This study was conducted on one-year duration, the subjects were chosen through the consecutive sampling method, all subjects were examined for genetics and resistance to clopidogrel. The data were analyzed through statistical analysis, a bivariate analysis was conducted to determine the correlation between several variables and the resistance variable. This study employed resistance diagnostic methods with VerifyNow. Polymorphism of receptor P2Y12 was tested with the Polymerase Chain Reaction method (PCR) and analysis of restriction fragment length polymorphism (RFLP). The genes tested in this study were G52T and C34T. Results: The number of participants in this study was 112. Examination of gene P2Y12 showed that the majority was homozygote, wild-type C34T allele (67%), and G52T (66.1%). There was no significant correlation between clopidogrel resistance and gene G52T and C34T of P2Y12 (p > 0.05). Hb levels significantly correlated with P2Y12 G52T (p = 0.024). Meanwhile, Fatty Liver significantly correlated with P2Y12 C34T (p = 0.037). Conclusion: Indonesia showed a low clopidogrel resistance rate and a very low C34T and G52T allele P2Y12 gene mutation, meaning that Indonesia had low mutations in the P2Y12. This is the cause of clopidogrel resistance in this study only 15%. Therefore, in a region with less clopidogrel resistance, examination of the P2Y12 gene would not give significant results.

Transactions of The Royal Society of Tropical Medicine and Hygiene, 1994

American Journal of Tropical Medicine and Hygiene, 2005

A prospective study of dengue fever (DF) and dengue hemorrhagic fever (DHF) was conducted in a co... more A prospective study of dengue fever (DF) and dengue hemorrhagic fever (DHF) was conducted in a cohort of adult volunteers from two textile factories located in West Java, Indonesia. Volunteers in the cohort were bled every three months and were actively followed for the occurrence of dengue (DEN) disease. The first two years of the study showed an incidence of symptomatic DEN disease of 18 cases per 1,000 person-years and an estimated asymptomatic/ mild infection rate of 56 cases per 1,000 person-years in areas of high disease transmission. In areas where no symptomatic cases were detected, the incidence of asymptomatic or mild infection was 8 cases per 1,000 person-years. Dengue-2 virus was the predominant serotype identified, but all four serotypes were detected among the cohort. Four cases of DHF and one case of dengue shock syndrome (DSS) were identified. Three of the four DHF cases were due to DEN-3 virus. The one DSS case occurred in the setting of a prior DEN-2 virus infection, followed by a secondary infection with DEN-1 virus. To our knowledge, this is the first report of a longitudinal cohort study of naturally acquired DF and DHF in adults.

The Egyptian Journal of Neurology, Psychiatry and Neurosurgery

Introduction Clopidogrel is recommended as an alternative to Acetyl Salicylic Acid (ASA), the fir... more Introduction Clopidogrel is recommended as an alternative to Acetyl Salicylic Acid (ASA), the first-line drug for secondary stroke prevention. Clopidogrel resistance in Indonesia is reportedly 15.8%. The Stent Thrombosis in Belgium (STIB) is a scoring system proposed to assess clopidogrel resistance which has not been tested for the reliability in Indonesian population. Objectives The objective of the study is to test the reliability of the STIB scoring and modified-STIB scoring system in Indonesian population. Methods This study was conducted cross-sectionally in Dr. Cipto Mangunkusumo Hospital and Universitas Indonesia Hospital from January 2020 to December 2021. Laboratory examinations of human CYP450 concentration in blood plasma from 112 subjects were carried out using the ELISA method. The clopidogrel resistance test was carried out using the VerifyNow method. Results Out of 112 ischemic stroke patients in this study, 14.3% of them did not respond to clopidogrel. Cross-tabulat...

ARDIMAS: Jurnal Arsip Pengabdian Masyarakat

The elderly are part of the natural stage of life as human beings. Some people will make various ... more The elderly are part of the natural stage of life as human beings. Some people will make various efforts to avoid the aging process. However, these efforts should be conducted as early as possible and cover the various factors involved in the aging process. A person in the aging process experiences various changes both physically and mentally, including a decline in both physical and physiological abilities. This community service activity is in the form of providing education about how to deal with their physical and physiological changes to get qualified and happy life, as well as simple health services. The implementation of the program takes place through the through the Community Partnership in "Improving the Quality of Life, for the Elderly through Family Assistance". The expected outcomes of this program are increased understanding of the occurrence of changes in the ability of physical activity and physiology in the elderly body, preparing for old age, and providin...

Frontiers in Cardiovascular Medicine

Despite patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention... more Despite patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) and receiving clopidogrel therapy, some patients still experience major adverse cardiovascular events (MACEs). Clopidogrel resistance, which may be regulated by genetic and epigenetic factors, may play a role in MACEs. This study aimed to determine the association between genetic (CYP2C19 and P2Y12 polymorphisms) and epigenetic (DNA methylation of CYP2C19 and P2Y12 and miRNA-26a expression) factors and their effects on MACEs among post-PCI patients. Post-PCI patients who received a standard dosage of clopidogrel at Harapan Kita Hospital between September 2018 and June 2020 were included in this study. MACEs were observed in patients within 1 year after PCI. Platelet aggregation was assessed using light transmission aggregometry (LTA). DNA methylation of CYP2C19 and P2Y12 was assessed using the bisulfite conversion method. CYP2C19 and P2Y12 polymorphisms and miRNA-26a expression we...

Vascular Health and Risk Management

Universitas Indonesia, 2008