Eun-mi Ahn - Academia.edu (original) (raw)

Papers by Eun-mi Ahn

Research paper thumbnail of Phenylpropanoids from Myristica fragrans Houtt

Journal of The Korean Society for Applied Biological Chemistry, 2004

Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing , EtOAc, n-... more Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing , EtOAc, n-BuOH and water, successively. The n-BuOH fraction gave three phenylpropanoids through application of silica gel column chromatographies. The chemical structures of the phenylpropanoids were determined by the interpretation of several spectral data, including NMR and MS as meso-dihydroguaiaretic acid (1), nectandrin B (2) and syringin methyl ether (3). Compound 1, which was first isolated from this plant by authors, showed inhibitory activities with on ACAT(acyl-CoA:Cholesterol Acyltransferase), chitin synthase III and HMG-CoA reductase (3-hydroxy-3-methylglutaryl coenzyme A reductase), respectively. Compound 3 showed inhibitory activities with on ACAT and chitin synthase III.

Research paper thumbnail of Isolation of monoamine oxidase B inhibitory compound from the leaves of Eucommia ulmoides Oliv

Applied Biological Chemistry, 1999

The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of ... more The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of Eucommia ulmoides leaves led to isolation of a flavonoid-glycoside inhibiting monoamine oxidase B activity. Its chemical structure was determined to be quercetin through interpretation of spectral data and adaptation of acid hydrolysis. The value of this compound in rat brain mitochondrial MAO-B inhibitory activity was evaluated to be .

Research paper thumbnail of Insecticidal alkaloids from the seeds of Macleaya cordata on cotton aphid (Aphis gossypii)

한국응용생명화학회지, Apr 1, 2013

Alcohol extracts from the seeds of Macleaya cordata have shown a significant insecticidal effect ... more Alcohol extracts from the seeds of Macleaya cordata have shown a significant insecticidal effect against the growth of the cotton aphid, Aphis gossypii Glover. In this study, we aimed to isolate the principal component of M. cordata to manifest its insecticidal effect on A. gossypii. The dried seeds of M. cordata were extracted with 80% aqueous methanol (MeOH), and the concentrated extracts were partitioned successively with ethyl acetate (EtOAc), n-butyl alcohol (n-BuOH), and H 2 O. During the search for an insecticidal compound against the aphids, activityguided fractionation led to the isolation of two alkaloids from an n-BuOH fraction through repeated silica gel column chromatographic separations. Based on NMR, MS, and IR spectroscopic data, the chemical structures of the compounds were determined to be 8hydroxydihydrochelerythrine (1) and 8-methoxydihydrosanguinarine (2) This is the first study in which 8-methoxydihydrosanguinarine was isolated from M. cordata. 8-Hydroxydihydrochelerythrine (1) and 8-methoxydihydrosanguinarine (2) decreased the survival of the cotton aphid by 76.1±7.9 and 73.6±14.6% at 100 ppm, respectively.

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-II. Isolation of Fatty Acids and Sterol Glycosides from the Leaves of Cucurbita moschata DucH

Applied Biological Chemistry, 1999

The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with ... more The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and , respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, , and two kinds of unsaturated fatty acids.

Research paper thumbnail of Strategies for Improvement of Agricultural Environment and Creation of the Venture Businesses from Agricultural Chemistry : Poster Session / Biotechnology & Foods : Isolation and Characterization of Antioxidative Components of Fructus Mume

International Symposium on Open Collaboration, 1998

Research paper thumbnail of Isolation of Secondary Metabolites from the Kalopanacis Cortex

한국작물학회 학술발표대회 논문집, Nov 1, 2007

Research paper thumbnail of Phenolic Glycosides Isolated from Safflower(Carthamus tinctorius L.) Seeds Increase the Alkaline Phosphatase(ALP) Activity of Human Osteoblast-like Cells

Food Science and Biotechnology, Oct 1, 2006

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-I. -Isolation of Major Components from the Tuber of Ipomoea batatas Lam

Applied Biological Chemistry, 1997

Research paper thumbnail of Insecticidal Alkaloids on Aphids from Corydalis turtschaninovii Tubers

한국응용생명화학회지, Jun 30, 2011

Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the conc... more Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was successively partitioned with n-hexane, ethyl acetate (EtOAc), butanol (n-BuOH), and H 2 O. An activity-guided fractionation to search for insecticidal compounds against aphids led to the isolation of six alkaloids from the n-hexane and EtOAc fractions using a repeated silica gel, an octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographic separations. Based on the spectroscopic data of NMR, mass spectroscopy (MS), and IR, the chemical structures of the compounds were determined to be: (+)-stylopine (1), (+)-corydaline (2), demethylcorydalmine (3), isocorypalmine (4), glaucine (5), and pseudoprotopine (6). These compounds showed significant insecticidal effects against Aphis gossypii, 69.

Research paper thumbnail of Isolation of daucosterol from the rice hull of Oryza sativa L

Applied Biological Chemistry, 1998

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-III. Isolation of Flavonoid-glycoside from the Allium monanthum Max

Applied Biological Chemistry, 2000

The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with ... more The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and , respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, , and two kinds of unsaturated fatty acids.

Research paper thumbnail of Antioxidant Activity of Isolated Compounds from the Heartwoods of Rhus verniciflua

Applied Biological Chemistry, 2007

The heartwoods of Rhus verniciflua was extracted with and the concentrated extract was partitione... more The heartwoods of Rhus verniciflua was extracted with and the concentrated extract was partitioned with , EtOAc, n-BuOH and , successively. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. They were determined as sinapyl aldehyde (1), 2,4-dihydroxybenzaldehyde (2), 2,4-dihydroxybenzoic acid (3) and 2,4-dihydroxyphenylglyoxylic acid (4) on the basis of spectroscopic data, respectively. Among the isolated compounds, sinapyl aldehyde and 2,4-dihydroxyphenylglyoxylic acid showed the strong antioxidative activity than artificial antioxidant BHT in DPPH radical scavenging activity.

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-XVII. Chemical Constituents from the Aerial Parts of Artemisia princeps PAMPANINI (Sajuarissuk)

Journal of The Korean Society for Applied Biological Chemistry, 2007

... Elmer, Massachusetts, USA)로 측정하였다. 녹는점은 Fisher-John's Melting Point Apparatus(Fisher Sci... more ... Elmer, Massachusetts, USA)로 측정하였다. 녹는점은 Fisher-John's Melting Point Apparatus(Fisher Scientific, Miami, USA)로 측정하였으며, EI/MS는 JMS-700(JEOL, Tokyo, Japan) 를 사용하여 측정하였다. UV lamp는 Spectroline ...

Research paper thumbnail of Anti-cancer Activity of Flavonoids from Aceriphyllum rossii

한국응용생명화학회지, 2008

The methanol extract from the aerial parts of Aceriphyllum rossii was fractionated into ethyl ace... more The methanol extract from the aerial parts of Aceriphyllum rossii was fractionated into ethyl acetate, n-BuOH and layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc and n-BuOH layers afforded five flavonol glycosides. They were identified as astragalin (1), kaempferol 3-O--L-rhamnopyranosyl (16)--D-glucopyranoside (2), rutin (3), kaempferol 3-O--L-rhamnopyranosyl (14)--L-rhamnopyranosyl 16)--D-glucopyranoside (4), and quercetin 3-O--L-rhamnopyranosyl (14)--L-rhamnopyranosyl (16)--D-glucopyranoside (5) on the basis of spectroscopic data. All of them showed an inhibition in farnesyl protein tranferase (FPTase) activity, and rutin (3) inhibited the growth of rat H-ras cell and the cell migration of human umbilical vein endothelial cells (HUVECs).

Research paper thumbnail of Phenolic Compounds from the Roots of Brassica rapa ssp. campestris

Chemistry of Natural Compounds, Nov 1, 2013

A new benzyl D-D-fructofuranoside (3), along with six known phenol compounds, benzyl-E-D-glucopyr... more A new benzyl D-D-fructofuranoside (3), along with six known phenol compounds, benzyl-E-D-glucopyranoside (1), dihydrosyringin (2), syringin (4), triandrin (5), phillyrin (6), and neoolivil-4-O-E-D-glucopyranoside (7), was isolated from the roots of Brassica rapa ssp. campestris L. for the first time. The structures of the compounds were established on the basis of NMR, MS, and IR spectroscopic data.

Research paper thumbnail of Anticoagulation and Anticancer Constituents from Eugenia caryophyllata Thunb

The Korean Journal of Medicinal Crop Science, Apr 1, 2007

From the EtOAc fraction of Eugenia caryophyllata, four compounds were isolated through activity-g... more From the EtOAc fraction of Eugenia caryophyllata, four compounds were isolated through activity-guided silica gel column chromatography. From the result of spectroscopic data including NMR, MS and IR, the chemical structures of the compounds were determined as 1-allyl-4-...

Research paper thumbnail of New Lignan and Flavonoids from Cornus kousa Burg. and Cytotoxicity on Human Cancer Cell Lines

한국작물학회 학술발표대회 논문집, Nov 1, 2007

Research paper thumbnail of Triterpenoids from Trapa pseudoincisa

Journal of Applied Biological Chemistry, 2007

Trapa pseudoincisa Nakai, an aquatic plant, was extracted with 80% aqueous MeOH, and the concentr... more Trapa pseudoincisa Nakai, an aquatic plant, was extracted with 80% aqueous MeOH, and the concentrated extract was successively partitioned with EtOAc, n-BuOH, and . The EtOAc fraction gave three compounds, which were isolated through the repeated silica gel and ODS column chromatographies. Based on the spectroscopic data obtained from NMR, MS, and IR, the chemical structures of the compounds were determined as cycloeucalenol (1), ursolic acid (2), and 2,3,23-trihydroxyurs-12-en-28-oic acid (3). These triterpenoids were isolated for the first time from Hydrocaryaceae plants including T. pseudoincisa NAKAI.

Research paper thumbnail of Insecticidal Alkaloids on Aphids from Corydalis turtschaninovii Tubers

Journal of the Korean Society for Applied Biological Chemistry, 2011

Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the conc... more Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was successively partitioned with n-hexane, ethyl acetate (EtOAc), butanol (n-BuOH), and H 2 O. An activity-guided fractionation to search for insecticidal compounds against aphids led to the isolation of six alkaloids from the n-hexane and EtOAc fractions using a repeated silica gel, an octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographic separations. Based on the spectroscopic data of NMR, mass spectroscopy (MS), and IR, the chemical structures of the compounds were determined to be: (+)-stylopine (1), (+)-corydaline (2), demethylcorydalmine (3), isocorypalmine (4), glaucine (5), and pseudoprotopine (6). These compounds showed significant insecticidal effects against Aphis gossypii, 69.

Research paper thumbnail of Erratum to: Cytotoxicity of Neolignans from Magnolia obovata Fruits

Journal of Applied Biological Chemistry, 2013

Research paper thumbnail of Phenylpropanoids from Myristica fragrans Houtt

Journal of The Korean Society for Applied Biological Chemistry, 2004

Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing , EtOAc, n-... more Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing , EtOAc, n-BuOH and water, successively. The n-BuOH fraction gave three phenylpropanoids through application of silica gel column chromatographies. The chemical structures of the phenylpropanoids were determined by the interpretation of several spectral data, including NMR and MS as meso-dihydroguaiaretic acid (1), nectandrin B (2) and syringin methyl ether (3). Compound 1, which was first isolated from this plant by authors, showed inhibitory activities with on ACAT(acyl-CoA:Cholesterol Acyltransferase), chitin synthase III and HMG-CoA reductase (3-hydroxy-3-methylglutaryl coenzyme A reductase), respectively. Compound 3 showed inhibitory activities with on ACAT and chitin synthase III.

Research paper thumbnail of Isolation of monoamine oxidase B inhibitory compound from the leaves of Eucommia ulmoides Oliv

Applied Biological Chemistry, 1999

The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of ... more The repeated silica gel colum chromatographies of n-BuOH fraction obtained from MeOH extracts of Eucommia ulmoides leaves led to isolation of a flavonoid-glycoside inhibiting monoamine oxidase B activity. Its chemical structure was determined to be quercetin through interpretation of spectral data and adaptation of acid hydrolysis. The value of this compound in rat brain mitochondrial MAO-B inhibitory activity was evaluated to be .

Research paper thumbnail of Insecticidal alkaloids from the seeds of Macleaya cordata on cotton aphid (Aphis gossypii)

한국응용생명화학회지, Apr 1, 2013

Alcohol extracts from the seeds of Macleaya cordata have shown a significant insecticidal effect ... more Alcohol extracts from the seeds of Macleaya cordata have shown a significant insecticidal effect against the growth of the cotton aphid, Aphis gossypii Glover. In this study, we aimed to isolate the principal component of M. cordata to manifest its insecticidal effect on A. gossypii. The dried seeds of M. cordata were extracted with 80% aqueous methanol (MeOH), and the concentrated extracts were partitioned successively with ethyl acetate (EtOAc), n-butyl alcohol (n-BuOH), and H 2 O. During the search for an insecticidal compound against the aphids, activityguided fractionation led to the isolation of two alkaloids from an n-BuOH fraction through repeated silica gel column chromatographic separations. Based on NMR, MS, and IR spectroscopic data, the chemical structures of the compounds were determined to be 8hydroxydihydrochelerythrine (1) and 8-methoxydihydrosanguinarine (2) This is the first study in which 8-methoxydihydrosanguinarine was isolated from M. cordata. 8-Hydroxydihydrochelerythrine (1) and 8-methoxydihydrosanguinarine (2) decreased the survival of the cotton aphid by 76.1±7.9 and 73.6±14.6% at 100 ppm, respectively.

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-II. Isolation of Fatty Acids and Sterol Glycosides from the Leaves of Cucurbita moschata DucH

Applied Biological Chemistry, 1999

The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with ... more The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and , respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, , and two kinds of unsaturated fatty acids.

Research paper thumbnail of Strategies for Improvement of Agricultural Environment and Creation of the Venture Businesses from Agricultural Chemistry : Poster Session / Biotechnology & Foods : Isolation and Characterization of Antioxidative Components of Fructus Mume

International Symposium on Open Collaboration, 1998

Research paper thumbnail of Isolation of Secondary Metabolites from the Kalopanacis Cortex

한국작물학회 학술발표대회 논문집, Nov 1, 2007

Research paper thumbnail of Phenolic Glycosides Isolated from Safflower(Carthamus tinctorius L.) Seeds Increase the Alkaline Phosphatase(ALP) Activity of Human Osteoblast-like Cells

Food Science and Biotechnology, Oct 1, 2006

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-I. -Isolation of Major Components from the Tuber of Ipomoea batatas Lam

Applied Biological Chemistry, 1997

Research paper thumbnail of Insecticidal Alkaloids on Aphids from Corydalis turtschaninovii Tubers

한국응용생명화학회지, Jun 30, 2011

Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the conc... more Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was successively partitioned with n-hexane, ethyl acetate (EtOAc), butanol (n-BuOH), and H 2 O. An activity-guided fractionation to search for insecticidal compounds against aphids led to the isolation of six alkaloids from the n-hexane and EtOAc fractions using a repeated silica gel, an octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographic separations. Based on the spectroscopic data of NMR, mass spectroscopy (MS), and IR, the chemical structures of the compounds were determined to be: (+)-stylopine (1), (+)-corydaline (2), demethylcorydalmine (3), isocorypalmine (4), glaucine (5), and pseudoprotopine (6). These compounds showed significant insecticidal effects against Aphis gossypii, 69.

Research paper thumbnail of Isolation of daucosterol from the rice hull of Oryza sativa L

Applied Biological Chemistry, 1998

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-III. Isolation of Flavonoid-glycoside from the Allium monanthum Max

Applied Biological Chemistry, 2000

The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with ... more The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and , respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, , and two kinds of unsaturated fatty acids.

Research paper thumbnail of Antioxidant Activity of Isolated Compounds from the Heartwoods of Rhus verniciflua

Applied Biological Chemistry, 2007

The heartwoods of Rhus verniciflua was extracted with and the concentrated extract was partitione... more The heartwoods of Rhus verniciflua was extracted with and the concentrated extract was partitioned with , EtOAc, n-BuOH and , successively. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. They were determined as sinapyl aldehyde (1), 2,4-dihydroxybenzaldehyde (2), 2,4-dihydroxybenzoic acid (3) and 2,4-dihydroxyphenylglyoxylic acid (4) on the basis of spectroscopic data, respectively. Among the isolated compounds, sinapyl aldehyde and 2,4-dihydroxyphenylglyoxylic acid showed the strong antioxidative activity than artificial antioxidant BHT in DPPH radical scavenging activity.

Research paper thumbnail of Development of Biologically Active Compounds from Edible Plant Sources-XVII. Chemical Constituents from the Aerial Parts of Artemisia princeps PAMPANINI (Sajuarissuk)

Journal of The Korean Society for Applied Biological Chemistry, 2007

... Elmer, Massachusetts, USA)로 측정하였다. 녹는점은 Fisher-John's Melting Point Apparatus(Fisher Sci... more ... Elmer, Massachusetts, USA)로 측정하였다. 녹는점은 Fisher-John's Melting Point Apparatus(Fisher Scientific, Miami, USA)로 측정하였으며, EI/MS는 JMS-700(JEOL, Tokyo, Japan) 를 사용하여 측정하였다. UV lamp는 Spectroline ...

Research paper thumbnail of Anti-cancer Activity of Flavonoids from Aceriphyllum rossii

한국응용생명화학회지, 2008

The methanol extract from the aerial parts of Aceriphyllum rossii was fractionated into ethyl ace... more The methanol extract from the aerial parts of Aceriphyllum rossii was fractionated into ethyl acetate, n-BuOH and layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc and n-BuOH layers afforded five flavonol glycosides. They were identified as astragalin (1), kaempferol 3-O--L-rhamnopyranosyl (16)--D-glucopyranoside (2), rutin (3), kaempferol 3-O--L-rhamnopyranosyl (14)--L-rhamnopyranosyl 16)--D-glucopyranoside (4), and quercetin 3-O--L-rhamnopyranosyl (14)--L-rhamnopyranosyl (16)--D-glucopyranoside (5) on the basis of spectroscopic data. All of them showed an inhibition in farnesyl protein tranferase (FPTase) activity, and rutin (3) inhibited the growth of rat H-ras cell and the cell migration of human umbilical vein endothelial cells (HUVECs).

Research paper thumbnail of Phenolic Compounds from the Roots of Brassica rapa ssp. campestris

Chemistry of Natural Compounds, Nov 1, 2013

A new benzyl D-D-fructofuranoside (3), along with six known phenol compounds, benzyl-E-D-glucopyr... more A new benzyl D-D-fructofuranoside (3), along with six known phenol compounds, benzyl-E-D-glucopyranoside (1), dihydrosyringin (2), syringin (4), triandrin (5), phillyrin (6), and neoolivil-4-O-E-D-glucopyranoside (7), was isolated from the roots of Brassica rapa ssp. campestris L. for the first time. The structures of the compounds were established on the basis of NMR, MS, and IR spectroscopic data.

Research paper thumbnail of Anticoagulation and Anticancer Constituents from Eugenia caryophyllata Thunb

The Korean Journal of Medicinal Crop Science, Apr 1, 2007

From the EtOAc fraction of Eugenia caryophyllata, four compounds were isolated through activity-g... more From the EtOAc fraction of Eugenia caryophyllata, four compounds were isolated through activity-guided silica gel column chromatography. From the result of spectroscopic data including NMR, MS and IR, the chemical structures of the compounds were determined as 1-allyl-4-...

Research paper thumbnail of New Lignan and Flavonoids from Cornus kousa Burg. and Cytotoxicity on Human Cancer Cell Lines

한국작물학회 학술발표대회 논문집, Nov 1, 2007

Research paper thumbnail of Triterpenoids from Trapa pseudoincisa

Journal of Applied Biological Chemistry, 2007

Trapa pseudoincisa Nakai, an aquatic plant, was extracted with 80% aqueous MeOH, and the concentr... more Trapa pseudoincisa Nakai, an aquatic plant, was extracted with 80% aqueous MeOH, and the concentrated extract was successively partitioned with EtOAc, n-BuOH, and . The EtOAc fraction gave three compounds, which were isolated through the repeated silica gel and ODS column chromatographies. Based on the spectroscopic data obtained from NMR, MS, and IR, the chemical structures of the compounds were determined as cycloeucalenol (1), ursolic acid (2), and 2,3,23-trihydroxyurs-12-en-28-oic acid (3). These triterpenoids were isolated for the first time from Hydrocaryaceae plants including T. pseudoincisa NAKAI.

Research paper thumbnail of Insecticidal Alkaloids on Aphids from Corydalis turtschaninovii Tubers

Journal of the Korean Society for Applied Biological Chemistry, 2011

Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the conc... more Tubers of Corydalis turtschaninovii were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was successively partitioned with n-hexane, ethyl acetate (EtOAc), butanol (n-BuOH), and H 2 O. An activity-guided fractionation to search for insecticidal compounds against aphids led to the isolation of six alkaloids from the n-hexane and EtOAc fractions using a repeated silica gel, an octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographic separations. Based on the spectroscopic data of NMR, mass spectroscopy (MS), and IR, the chemical structures of the compounds were determined to be: (+)-stylopine (1), (+)-corydaline (2), demethylcorydalmine (3), isocorypalmine (4), glaucine (5), and pseudoprotopine (6). These compounds showed significant insecticidal effects against Aphis gossypii, 69.

Research paper thumbnail of Erratum to: Cytotoxicity of Neolignans from Magnolia obovata Fruits

Journal of Applied Biological Chemistry, 2013