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Papers by Fabiana Tonial

European Journal of Plant Pathology

Brazilian Journal of Animal and Environmental Research, 2022

A busca por materiais sustentáveis e de desempenho semelhante ao cimento tem aumentado, visto as ... more A busca por materiais sustentáveis e de desempenho semelhante ao cimento tem aumentado, visto as complicações ambientais causadas pela produção do material. Uma das opções de material “substituto” são os microrganismos produtores de uréase, capazes de precipitar carbonato de cálcio, responsável pela cristalização. Esses microrganismos podem ser utilizados de diferentes formas para aumentar a resistência de argamassas. A biodeposição é o processo de aspersão de uma solução bacteriana em corpos de prova endurecidos. Neste estudo foram realizadas aspersões semanais de solução bacteriana em amostras de argamassa, e deixadas outras amostras como referência, com aspersão apenas de água, com o objetivo de verificar se a precipitação de carbonato de cálcio produziria aumento na resistência da argamassa. Ao romper os exemplares à compressão e tração, verificou-se aumento significativo nos que tiveram adição de bactérias, indicando que houve preenchimento de poros.

Each fasta file represents a protein from the core proteome of Paenibacillus species.These orthol... more Each fasta file represents a protein from the core proteome of Paenibacillus species.These ortholog groups were generated through the Get_homologues package (OrthoMCL option).

Anais do I Congresso Brasileiro de Parasitologia Humana On-line, 2021

Introdução: O Trichomonas vaginalis é um protozoário flagelado causador de doenças, sobretudo em ... more Introdução: O Trichomonas vaginalis é um protozoário flagelado causador de doenças, sobretudo em órgãos genitais femininos. A principal delas é a Tricomoníase, mas também pode causar vaginites. Por isso, conhecer o melhor tratamento e os mecanismos para evitar futuras reinfecções pelo T. vaginalis é importante no estudo da parasitologia humana. Objetivo: Avaliar, por meio de revisão de literatura, o melhor tratamento e a como prevenir a (re)infecção de Trichomonas vaginalis em mulheres. Material e métodos: Realizou-se busca na base de dados PubMed utilizando os descritores “Trichomonas vaginalis”, “treatment”, “prevention” e “woman”. Como critérios de inclusão, estabeleceu-se artigos publicados nos últimos 10 anos a partir de janeiro de 2021 e relacionados a Ensaios Clínicos, com texto completo gratuitamente. Resultados: A ideia fundamental sugere que a prevenção deve ser aliada ao tratamento, pois o risco de reinfecção é alto, e ela deve ser realizada com uso de preservativos, prin...

Current Biotechnology, 2018

Background: Endophytic fungi isolated from medicinal plants have been recognized as promising sou... more Background: Endophytic fungi isolated from medicinal plants have been recognized as promising sources of highly variable bioactive metabolites. Maytenus ilicifolia, a highly exploited medicinal plant commonly used in popular medicine, is included in the current list of endangered species. Our research group is particularly interested in bioprospecting endophytes from medicinal plants found in Brazil, to control citrus diseases. In this context, Phyllosticta citricarpa is an agronomically important fungus associated with Citrus Black Spot (CBS), a disease associated with large economic losses, mainly due the phytosanitary restrictions for CBS disease in Europe. Objective: The objective of this study was to identify and explore the activity of two endophytic isolates from the medicinal plant M. ilicifolia against P. citricarpa, and to develop a reliable and efficient transformation system for the endophytic strains. Methods: During a screening program on the biodiversity of M. ilicifo...

Comunicata Scientiae, 2017

This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The e... more This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The extracts (plant material: solvent, 1:10) were prepared by maceration with methanol:water (1:1) during ten days followed by a concentration in a rotary evaporator under reduced pressure. The extracts were resuspended in water containing 1% of dimethylsulfoxide (DMSO) to obtain a final concentration of 100 mg/mL and then filtered through a sterilizing membrane with 0.22μm. The antibacterial activity of the leaf and root extracts were evaluated against pathogenic and phytopathogenic bacteria by agar well diffusion and microdilution broth methods for the minimum inhibitory concentrations (MIC) determination. The antifungal activity of tough lovegrass leaf and root extracts were evaluated by micelial growth inhibition and conidial germination inhibition. The extracts presented low antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Xanthomonas translucens, but...

Orientadora : Profª Drª Chirlei GlienkeTese (doutorado) - Universidade Federal do Paraná, Setor d... more Orientadora : Profª Drª Chirlei GlienkeTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Ciencias Biológicas (Microbiologia, Parasitologia e Patologia Básica). Defesa: Curitiba, 29/09/2014Inclui bibliografiaResumo: A obtenção de novos compostos bioativos com diferentes mecanismos de ação, menores efeitos indesejáveis, maior segurança na utilização e maior eficácia é uma necessidade constante na saúde, agronomia e indústria. Essa busca é indiscutivelmente importante tratando-se de compostos antimicrobianos devido a capacidade dos microrganismos tornarem-se resistentes aos mesmos. A bioprospecção é uma das ferramentas utilizadas para a obtenção de produtos naturais com essa ação. Uma das fontes promissoras utilizadas nesse tipo de investigação são os metabólitos secundários produzidos por microrganismos endofíticos obtidos a partir de plantas medicinais. Deste modo, o trabalho a seguir teve como propósito a obtenção de produtos bioativos produzidos por fungos endofíticos das folhas de Schinus terebinthifolius, árvore popularmente conhecida como aroeira. O trabalho de Bioprospecção de Metabólitos Secundários de Endófitos de S. terebinthifolius foi dividido em dois capítulos voltados à otimização da produção de extratos ativos. O primeiro objetiva a obtenção de compostos contra patógenos humanos e o segundo contra o fitopatógeno Phyllosticta citricarpa. O primeiro capítulo, intitulado "Antimicrobial potential of endophytic fungi from Schinus terebinthifolius", explorou a capacidade de produção de extratos de interesse a partir de três fungos pertencentes aos gêneros Alternaria, Bjerkandera e Xylaria, sob diferentes condições de cultivo, comprovando a influência das fontes de carbono e nitrogênio, pH e temperatura sobre o metabolismo secundário dos microrganismos. A obtenção do extrato do fungo Alternaria sp. Sect. Alternata teve a escala ampliada e o mesmo foi avaliado frente à bactéria Staphylococcus aureus resistente à meticilina (MRSA). Duas porções do extrato bruto foram ativas contra essa bactéria. Hexadecanoato de metila e um alcaloide pirrolopirazina foram identificados nas frações ativas do isolado de Alternaria sp. Sect. Alternata. O segundo estudo, "Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa", possibilitou a obtenção de uma fração biologicamente ativa contra o fitopatógeno. Da biodiversidade fúngica estudada dois isolados de D. terebinthifolii foram os mais promissores contra P. citricarpa. Diferentes meios de cultivo foram avaliados na seleção do isolado mais eficiente, o qual foi escolhido para a produção do extrato em maior escala. O meio sólido arroz foi o mais apropriado. Foram identificados alguns compostos de interesse produzidos pelo endófito: álcool fenetílico, verbanol, hexadecanoato de metila e acetato de verbenil. De modo geral, os fungos endofíticos isolados das folhas de aroeira apresentaram interessante potencial biotecnológico, tanto para a área médica como agronômica, pela produção de metabólitos secundários com atividade antimicrobiana. Palavras-chave: endófitos - metabólitos secundários - Schinus terebinthifolius - atividade antimicrobianaAbstract: The obtainment of new bioactive compounds with different mechanisms of action, minor side effects, more effective and safest is a constant need in health, agronomy and industry. This search is unquestionably important in the case of antimicrobial compounds because of the ability of microorganisms become resistant to them. The bioprospection is one of the tools used to obtain natural products with this action. One of the promising sources used in this type of investigation are secondary metabolites produced by endophytic microorganisms obtained from medicinal plants. Thus, the following work had as purpose obtaining bioactive products produced by endophytic fungus from the leaves of Schinus terebinthifolius, tree popularly known as aroeira. The work "Secondary Metabolites Bioprospection of S. terebinthifolius Endophytes" was divided into two chapters. The first one focused in the optimization of active extracts production against human pathogens and the second in the obtainment of a product against the phytopathogen Phyllosticta citricarpa. The first chapter, entitled "Antimicrobial potential of endophytic fungi from Schinus terebinthifolius", explored the capacity of production of interest extracts using three fungi belonging to the genera Alternaria, Bjerkandera and Xylaria under different culture conditions demonstrating the influence of carbon and nitrogen sources, pH and temperature on the secondary metabolism of these microorganisms. The scale production of Alternaria sp. Sect. Alternata extract was expanded and the product has been evaluated against the methicillin-resistant Staphylococcus aureus (MRSA). Two active portions against the microorganism were isolated from the crude extract. Methyl hexadecanoate and a…

Current Microbiology, 2015

In this study, we analyzed the antimicrobial activity of extracts harvested from 17 endophytic fu... more In this study, we analyzed the antimicrobial activity of extracts harvested from 17 endophytic fungi isolated from the medicinal plant Schinus terebinthifolius. Morphological and molecular analyses indicated that these fungal species belonged to the genera Alternaria, Bjerkandera, Colletotrichum, Diaporthe, Penicillium, and Xylaria. Of the endophytes analyzed, 64.7 % produced antimicrobial compounds under at least one of the fermentation conditions tested. Nine isolates produced compounds that inhibited growth of Staphylococcus aureus, four produced compounds that inhibited Candida albicans, and two that inhibited Pseudomonas aeruginosa. The fermentation conditions of the following endophytes were optimized: Alternaria sp. Sect. Alternata-LGMF626, Xylaria sp.-LGMF673, and Bjerkandera sp.-LGMF713. Specifically, the carbon and nitrogen sources, initial pH, temperature, and length of incubation were varied. In general, production of antimicrobial compounds was greatest when galactose was used as a carbon source, and acidification of the growth medium enhanced the production of compounds that inhibited C. albicans. Upon largescale fermentation, Alternaria sp. Sect. Alternata-LGMF626 produced an extract containing two fractions that were active against methicillin-resistant S. aureus. One of the extracts exhibited high activity (minimum inhibitory concentration of 18.52 lg/mL), and the other exhibited moderate activity (minimum inhibitory concentration of 55.55 lg/mL). The compounds E-2-hexyl-cinnamaldehyde and two compounds of the pyrrolopyrazine alkaloids class were identified in the active fractions by gas chromatography-mass spectrometry.

Brazilian Journal of Pharmaceutical Sciences, 2014

In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially l... more In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were examined concerning proteolytic activity. All studied species have proteins with significant proteolytic activity and E. papillosa has the greatest specific activity. Aiming to verify the type of protease present, an assay with different inhibitors was performed. In the three tested plants, the proteolytic activity was significantly inhibited by a serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF). Using techniques of electrophoresis with polyacrylamide gels (SDS-PAGE), the subunits of proteins were separated according to their molecular masses, and the protein activity was visually detected by zymography.

Anais da Academia Brasileira de Ciências, 2014

The phytochemical profile and antimicrobial activity of cultivar (cv.) extracts of Lotus uliginos... more The phytochemical profile and antimicrobial activity of cultivar (cv.) extracts of Lotus uliginosus (cvs. Trojan and Serrano), L. tenuis (cv. Larrañaga) and L. corniculatus (cv. São Gabriel) were investigated. The phytochemical analysis revealed tannins, coumarins and flavonoids in all extracts, with variations among cultivars, showing genotypic variability. By High Performance Liquid Chromatographic method, the cvs. Larrañaga and São Gabriel showed the highest percentage of catechin and epicatechin, respectively, and presented rutin, which was not detected in the other ones. These genotypes showed antifungal activity but not antibacterial one. The cv. Larrañaga inhibited the mycelia growth of Alternaria sp. and Fusarium graminearum while the cv. São Gabriel was active only against Alternaria sp. The cultivars showed the greatest amounts of secondary metabolites and demonstrated significant activity against filamentous fungi. The results provide a direction for further research abou...

Archives of Oral Biology, 2014

Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofi... more Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofilm formation of Candida albicans and Streptococcus mutans

Antimicrobial Agents, 2012

Revista de Medicina, Mar 25, 2022

Paracoccidioidomycosis (PCM) is not a notifiable disease despite its relevance in Latin America, ... more Paracoccidioidomycosis (PCM) is not a notifiable disease despite its relevance in Latin America, and therefore estimates of the prevalence, incidence, and associated morbidity of this mycosis are based on reports of epidemiological surveys, case series, hospitalization records, and mortality data. The objective of this study was to describe aspects related to the patient, disease evolution, diagnostic confirmation, and treatment of confirmed cases of PCM treated at a teaching hospital in southern Brazil. Information was collected from the medical records of 27 patients diagnosed with PCM, confirmed in the period from 2010 to 2019. The prevalent profile was a male patient, with a mean age of 53 years, who was involved in various work activities, of urban origin, immunocompetent and without comorbidities, and a smoker, but not an alcoholic. For most cases, the initial involvement was pulmonary, with significant involvement of the lymphatic system during the course of the disease. Microscopic observation of pathognomonic fungal structures in biopsy samples, lymph node aspirates, and sputum was the most common method to confirm the clinical suspicion. Itraconazole was the first treatment option, followed by amphotericin B.

Este trabalho teve como objetivo, isolar bacterias do genero Bacillus, produtoras de urease, a pa... more Este trabalho teve como objetivo, isolar bacterias do genero Bacillus, produtoras de urease, a partir de amostras de solo, a fim de realizar um levantamento do potencial destas para o uso em biocimentacao. A amostra de solo foi extraida de um latossolo argiloso, no horizonte B, diluida em solucao salina e submetida a temperatura de 80ᵒC, afim de selecionar apenas as colonias bacterianas produtoras de urease. Da solucao foi produzida uma biomassa, em forma solida, onde as colonias ficaram esporuladas, apos foram revitalizadas para a utilizacao na confeccao de argamassa. Um teste piloto com a utilizacao das bacterias em substituicao ao cimento na argamassa foi realizado, confeccionado corpos de prova e que apos 28 dias foram testadas suas resistencias a compressao e tracao na flexao, os resultados nao atingiram as resistencias minimas estabelecidas pelas normas tecnicas, mas pode-se observar que houve atividade pozolânica, pois a amostra sem o uso de bacterias ficou 50% menos resisten...

Journal of Thermal Analysis and Calorimetry

Lactobacillus fermentum is a probiotic that belongs to the group of lactic acid bacteria and pres... more Lactobacillus fermentum is a probiotic that belongs to the group of lactic acid bacteria and presents high intestinal colonization capacity after oral administration. To maintain its quality during manufacturing to its expiration, the physical and chemical stability of the probiotic remains a major challenge and concern. This work aimed to determine the thermal degradation parameters of the probiotic Lactobacillus fermentum by thermogravimetry (TG), calculated using the Arrhenius mathematical model. The probiotic degradation followed a second-order kinetics, where the rate of its degradation depends on the concentration of two or more reactants, presenting degradation rate constant of 6.23 × 10−10 min−1 at 25 °C and an activation energy of 21,987 cal mol−1. The value corresponding to the simulate shelf life was also obtained. TG is an extremely useful tool, simple and fast to determine the degradation rate parameters of the compounds including pharmaceuticals and nutraceuticals. The shelf life needs to be defined by realist data. This is the first time that was used TG to determine the degradation reaction of probiotics.

O Mundo da Saúde

The microbiological contamination of pharmaceutical and cosmetic products represents a public hea... more The microbiological contamination of pharmaceutical and cosmetic products represents a public health problem because it can cause infections. Before purchasing a particular cosmetic, many consumers test it on their own skin, increasing the susceptibility to microbiological contaminations. We hypothesize that these lipstick testers available to pharmacy consumers represent a potential source of microbiological contamination. This study analyzed the microbiological quality of 30 lipstick samples, randomly collected, from different manufacturers, available to the consumers for trials in pharmacies in southern Brazil (fifteen samples were collected in Casca, five in Ciríaco and ten in Passo Fundo). The microbiological quality of the lipsticks was evaluated by: total count of viable bacteria and mold and yeast, and presence of pathogens Staphylococcus aureus and Pseudomonas aeruginosa. It was verified that amounts ranged from 1.0 x 10¹ to 1.9 x 105 CFU/g of viable bacteria and from 1.0 x 10¹ to 7.3 x 10³ CFU/g of molds and yeasts on the lipsticks. 54.33% and 40% of the samples were disapproved by the total count of viable bacteria and molds and yeasts, respectively. S. aureus, Aspergillus sp. and Cladosporium sp. were also found. Although the waxy composition of the lipsticks hinders microbial contamination this research reveals a large number of microorganisms. Our hypothesis that the lipsticks have a high microbiological load was confirmed. Opportunistic pathogenic microorganisms can become infectious agents in patients with compromised immune systems. The use of disposable applicators is proposed as an alternative way to avoid microbiological contamination of the cosmetic products.

Revista Verde de Agroecologia e Desenvolvimento Sustentável

Climate change can cause imbalances in plants. Baccharis trimera is a specie usually found in har... more Climate change can cause imbalances in plants. Baccharis trimera is a specie usually found in harsh conditions and has medicinal and agricultural properties. Thus, this study aimed to evaluate the biochemical and physiological responses of this plant under water restriction (WR) levels. B. trimera samples were identified and collected in Erechim/RS and propagated in a greenhouse. When acclimated, they were subjected to 0, 25, 75 and 100% WR, determined according to the water saturation in the soil by capillary action. After 30 days of treatment, the physiological responses: growth, and fresh and dry biomass; and the biochemical responses: activity of superoxide dismutase (SOD), guaiacol peroxidase (GP) and ascorbate peroxidase (APX) enzymes, proline, protein and hydrogen peroxide content, and lipid peroxidation, were determined. Data were submitted to regression analysis and Pearson correlation. The WR of 27.37%, on average, induced an increase in physiological parameters, but the r...

European Journal of Plant Pathology

Brazilian Journal of Animal and Environmental Research, 2022

A busca por materiais sustentáveis e de desempenho semelhante ao cimento tem aumentado, visto as ... more A busca por materiais sustentáveis e de desempenho semelhante ao cimento tem aumentado, visto as complicações ambientais causadas pela produção do material. Uma das opções de material “substituto” são os microrganismos produtores de uréase, capazes de precipitar carbonato de cálcio, responsável pela cristalização. Esses microrganismos podem ser utilizados de diferentes formas para aumentar a resistência de argamassas. A biodeposição é o processo de aspersão de uma solução bacteriana em corpos de prova endurecidos. Neste estudo foram realizadas aspersões semanais de solução bacteriana em amostras de argamassa, e deixadas outras amostras como referência, com aspersão apenas de água, com o objetivo de verificar se a precipitação de carbonato de cálcio produziria aumento na resistência da argamassa. Ao romper os exemplares à compressão e tração, verificou-se aumento significativo nos que tiveram adição de bactérias, indicando que houve preenchimento de poros.

Each fasta file represents a protein from the core proteome of Paenibacillus species.These orthol... more Each fasta file represents a protein from the core proteome of Paenibacillus species.These ortholog groups were generated through the Get_homologues package (OrthoMCL option).

Anais do I Congresso Brasileiro de Parasitologia Humana On-line, 2021

Introdução: O Trichomonas vaginalis é um protozoário flagelado causador de doenças, sobretudo em ... more Introdução: O Trichomonas vaginalis é um protozoário flagelado causador de doenças, sobretudo em órgãos genitais femininos. A principal delas é a Tricomoníase, mas também pode causar vaginites. Por isso, conhecer o melhor tratamento e os mecanismos para evitar futuras reinfecções pelo T. vaginalis é importante no estudo da parasitologia humana. Objetivo: Avaliar, por meio de revisão de literatura, o melhor tratamento e a como prevenir a (re)infecção de Trichomonas vaginalis em mulheres. Material e métodos: Realizou-se busca na base de dados PubMed utilizando os descritores “Trichomonas vaginalis”, “treatment”, “prevention” e “woman”. Como critérios de inclusão, estabeleceu-se artigos publicados nos últimos 10 anos a partir de janeiro de 2021 e relacionados a Ensaios Clínicos, com texto completo gratuitamente. Resultados: A ideia fundamental sugere que a prevenção deve ser aliada ao tratamento, pois o risco de reinfecção é alto, e ela deve ser realizada com uso de preservativos, prin...

Current Biotechnology, 2018

Background: Endophytic fungi isolated from medicinal plants have been recognized as promising sou... more Background: Endophytic fungi isolated from medicinal plants have been recognized as promising sources of highly variable bioactive metabolites. Maytenus ilicifolia, a highly exploited medicinal plant commonly used in popular medicine, is included in the current list of endangered species. Our research group is particularly interested in bioprospecting endophytes from medicinal plants found in Brazil, to control citrus diseases. In this context, Phyllosticta citricarpa is an agronomically important fungus associated with Citrus Black Spot (CBS), a disease associated with large economic losses, mainly due the phytosanitary restrictions for CBS disease in Europe. Objective: The objective of this study was to identify and explore the activity of two endophytic isolates from the medicinal plant M. ilicifolia against P. citricarpa, and to develop a reliable and efficient transformation system for the endophytic strains. Methods: During a screening program on the biodiversity of M. ilicifo...

Comunicata Scientiae, 2017

This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The e... more This study aimed to evaluate tough lovegrass leaf and root extracts antimicrobial activity. The extracts (plant material: solvent, 1:10) were prepared by maceration with methanol:water (1:1) during ten days followed by a concentration in a rotary evaporator under reduced pressure. The extracts were resuspended in water containing 1% of dimethylsulfoxide (DMSO) to obtain a final concentration of 100 mg/mL and then filtered through a sterilizing membrane with 0.22μm. The antibacterial activity of the leaf and root extracts were evaluated against pathogenic and phytopathogenic bacteria by agar well diffusion and microdilution broth methods for the minimum inhibitory concentrations (MIC) determination. The antifungal activity of tough lovegrass leaf and root extracts were evaluated by micelial growth inhibition and conidial germination inhibition. The extracts presented low antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Xanthomonas translucens, but...

Orientadora : Profª Drª Chirlei GlienkeTese (doutorado) - Universidade Federal do Paraná, Setor d... more Orientadora : Profª Drª Chirlei GlienkeTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Ciencias Biológicas (Microbiologia, Parasitologia e Patologia Básica). Defesa: Curitiba, 29/09/2014Inclui bibliografiaResumo: A obtenção de novos compostos bioativos com diferentes mecanismos de ação, menores efeitos indesejáveis, maior segurança na utilização e maior eficácia é uma necessidade constante na saúde, agronomia e indústria. Essa busca é indiscutivelmente importante tratando-se de compostos antimicrobianos devido a capacidade dos microrganismos tornarem-se resistentes aos mesmos. A bioprospecção é uma das ferramentas utilizadas para a obtenção de produtos naturais com essa ação. Uma das fontes promissoras utilizadas nesse tipo de investigação são os metabólitos secundários produzidos por microrganismos endofíticos obtidos a partir de plantas medicinais. Deste modo, o trabalho a seguir teve como propósito a obtenção de produtos bioativos produzidos por fungos endofíticos das folhas de Schinus terebinthifolius, árvore popularmente conhecida como aroeira. O trabalho de Bioprospecção de Metabólitos Secundários de Endófitos de S. terebinthifolius foi dividido em dois capítulos voltados à otimização da produção de extratos ativos. O primeiro objetiva a obtenção de compostos contra patógenos humanos e o segundo contra o fitopatógeno Phyllosticta citricarpa. O primeiro capítulo, intitulado "Antimicrobial potential of endophytic fungi from Schinus terebinthifolius", explorou a capacidade de produção de extratos de interesse a partir de três fungos pertencentes aos gêneros Alternaria, Bjerkandera e Xylaria, sob diferentes condições de cultivo, comprovando a influência das fontes de carbono e nitrogênio, pH e temperatura sobre o metabolismo secundário dos microrganismos. A obtenção do extrato do fungo Alternaria sp. Sect. Alternata teve a escala ampliada e o mesmo foi avaliado frente à bactéria Staphylococcus aureus resistente à meticilina (MRSA). Duas porções do extrato bruto foram ativas contra essa bactéria. Hexadecanoato de metila e um alcaloide pirrolopirazina foram identificados nas frações ativas do isolado de Alternaria sp. Sect. Alternata. O segundo estudo, "Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa", possibilitou a obtenção de uma fração biologicamente ativa contra o fitopatógeno. Da biodiversidade fúngica estudada dois isolados de D. terebinthifolii foram os mais promissores contra P. citricarpa. Diferentes meios de cultivo foram avaliados na seleção do isolado mais eficiente, o qual foi escolhido para a produção do extrato em maior escala. O meio sólido arroz foi o mais apropriado. Foram identificados alguns compostos de interesse produzidos pelo endófito: álcool fenetílico, verbanol, hexadecanoato de metila e acetato de verbenil. De modo geral, os fungos endofíticos isolados das folhas de aroeira apresentaram interessante potencial biotecnológico, tanto para a área médica como agronômica, pela produção de metabólitos secundários com atividade antimicrobiana. Palavras-chave: endófitos - metabólitos secundários - Schinus terebinthifolius - atividade antimicrobianaAbstract: The obtainment of new bioactive compounds with different mechanisms of action, minor side effects, more effective and safest is a constant need in health, agronomy and industry. This search is unquestionably important in the case of antimicrobial compounds because of the ability of microorganisms become resistant to them. The bioprospection is one of the tools used to obtain natural products with this action. One of the promising sources used in this type of investigation are secondary metabolites produced by endophytic microorganisms obtained from medicinal plants. Thus, the following work had as purpose obtaining bioactive products produced by endophytic fungus from the leaves of Schinus terebinthifolius, tree popularly known as aroeira. The work "Secondary Metabolites Bioprospection of S. terebinthifolius Endophytes" was divided into two chapters. The first one focused in the optimization of active extracts production against human pathogens and the second in the obtainment of a product against the phytopathogen Phyllosticta citricarpa. The first chapter, entitled "Antimicrobial potential of endophytic fungi from Schinus terebinthifolius", explored the capacity of production of interest extracts using three fungi belonging to the genera Alternaria, Bjerkandera and Xylaria under different culture conditions demonstrating the influence of carbon and nitrogen sources, pH and temperature on the secondary metabolism of these microorganisms. The scale production of Alternaria sp. Sect. Alternata extract was expanded and the product has been evaluated against the methicillin-resistant Staphylococcus aureus (MRSA). Two active portions against the microorganism were isolated from the crude extract. Methyl hexadecanoate and a…

Current Microbiology, 2015

In this study, we analyzed the antimicrobial activity of extracts harvested from 17 endophytic fu... more In this study, we analyzed the antimicrobial activity of extracts harvested from 17 endophytic fungi isolated from the medicinal plant Schinus terebinthifolius. Morphological and molecular analyses indicated that these fungal species belonged to the genera Alternaria, Bjerkandera, Colletotrichum, Diaporthe, Penicillium, and Xylaria. Of the endophytes analyzed, 64.7 % produced antimicrobial compounds under at least one of the fermentation conditions tested. Nine isolates produced compounds that inhibited growth of Staphylococcus aureus, four produced compounds that inhibited Candida albicans, and two that inhibited Pseudomonas aeruginosa. The fermentation conditions of the following endophytes were optimized: Alternaria sp. Sect. Alternata-LGMF626, Xylaria sp.-LGMF673, and Bjerkandera sp.-LGMF713. Specifically, the carbon and nitrogen sources, initial pH, temperature, and length of incubation were varied. In general, production of antimicrobial compounds was greatest when galactose was used as a carbon source, and acidification of the growth medium enhanced the production of compounds that inhibited C. albicans. Upon largescale fermentation, Alternaria sp. Sect. Alternata-LGMF626 produced an extract containing two fractions that were active against methicillin-resistant S. aureus. One of the extracts exhibited high activity (minimum inhibitory concentration of 18.52 lg/mL), and the other exhibited moderate activity (minimum inhibitory concentration of 55.55 lg/mL). The compounds E-2-hexyl-cinnamaldehyde and two compounds of the pyrrolopyrazine alkaloids class were identified in the active fractions by gas chromatography-mass spectrometry.

Brazilian Journal of Pharmaceutical Sciences, 2014

In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially l... more In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were examined concerning proteolytic activity. All studied species have proteins with significant proteolytic activity and E. papillosa has the greatest specific activity. Aiming to verify the type of protease present, an assay with different inhibitors was performed. In the three tested plants, the proteolytic activity was significantly inhibited by a serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF). Using techniques of electrophoresis with polyacrylamide gels (SDS-PAGE), the subunits of proteins were separated according to their molecular masses, and the protein activity was visually detected by zymography.

Anais da Academia Brasileira de Ciências, 2014

The phytochemical profile and antimicrobial activity of cultivar (cv.) extracts of Lotus uliginos... more The phytochemical profile and antimicrobial activity of cultivar (cv.) extracts of Lotus uliginosus (cvs. Trojan and Serrano), L. tenuis (cv. Larrañaga) and L. corniculatus (cv. São Gabriel) were investigated. The phytochemical analysis revealed tannins, coumarins and flavonoids in all extracts, with variations among cultivars, showing genotypic variability. By High Performance Liquid Chromatographic method, the cvs. Larrañaga and São Gabriel showed the highest percentage of catechin and epicatechin, respectively, and presented rutin, which was not detected in the other ones. These genotypes showed antifungal activity but not antibacterial one. The cv. Larrañaga inhibited the mycelia growth of Alternaria sp. and Fusarium graminearum while the cv. São Gabriel was active only against Alternaria sp. The cultivars showed the greatest amounts of secondary metabolites and demonstrated significant activity against filamentous fungi. The results provide a direction for further research abou...

Archives of Oral Biology, 2014

Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofi... more Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofilm formation of Candida albicans and Streptococcus mutans

Antimicrobial Agents, 2012

Revista de Medicina, Mar 25, 2022

Paracoccidioidomycosis (PCM) is not a notifiable disease despite its relevance in Latin America, ... more Paracoccidioidomycosis (PCM) is not a notifiable disease despite its relevance in Latin America, and therefore estimates of the prevalence, incidence, and associated morbidity of this mycosis are based on reports of epidemiological surveys, case series, hospitalization records, and mortality data. The objective of this study was to describe aspects related to the patient, disease evolution, diagnostic confirmation, and treatment of confirmed cases of PCM treated at a teaching hospital in southern Brazil. Information was collected from the medical records of 27 patients diagnosed with PCM, confirmed in the period from 2010 to 2019. The prevalent profile was a male patient, with a mean age of 53 years, who was involved in various work activities, of urban origin, immunocompetent and without comorbidities, and a smoker, but not an alcoholic. For most cases, the initial involvement was pulmonary, with significant involvement of the lymphatic system during the course of the disease. Microscopic observation of pathognomonic fungal structures in biopsy samples, lymph node aspirates, and sputum was the most common method to confirm the clinical suspicion. Itraconazole was the first treatment option, followed by amphotericin B.

Este trabalho teve como objetivo, isolar bacterias do genero Bacillus, produtoras de urease, a pa... more Este trabalho teve como objetivo, isolar bacterias do genero Bacillus, produtoras de urease, a partir de amostras de solo, a fim de realizar um levantamento do potencial destas para o uso em biocimentacao. A amostra de solo foi extraida de um latossolo argiloso, no horizonte B, diluida em solucao salina e submetida a temperatura de 80ᵒC, afim de selecionar apenas as colonias bacterianas produtoras de urease. Da solucao foi produzida uma biomassa, em forma solida, onde as colonias ficaram esporuladas, apos foram revitalizadas para a utilizacao na confeccao de argamassa. Um teste piloto com a utilizacao das bacterias em substituicao ao cimento na argamassa foi realizado, confeccionado corpos de prova e que apos 28 dias foram testadas suas resistencias a compressao e tracao na flexao, os resultados nao atingiram as resistencias minimas estabelecidas pelas normas tecnicas, mas pode-se observar que houve atividade pozolânica, pois a amostra sem o uso de bacterias ficou 50% menos resisten...

Journal of Thermal Analysis and Calorimetry

Lactobacillus fermentum is a probiotic that belongs to the group of lactic acid bacteria and pres... more Lactobacillus fermentum is a probiotic that belongs to the group of lactic acid bacteria and presents high intestinal colonization capacity after oral administration. To maintain its quality during manufacturing to its expiration, the physical and chemical stability of the probiotic remains a major challenge and concern. This work aimed to determine the thermal degradation parameters of the probiotic Lactobacillus fermentum by thermogravimetry (TG), calculated using the Arrhenius mathematical model. The probiotic degradation followed a second-order kinetics, where the rate of its degradation depends on the concentration of two or more reactants, presenting degradation rate constant of 6.23 × 10−10 min−1 at 25 °C and an activation energy of 21,987 cal mol−1. The value corresponding to the simulate shelf life was also obtained. TG is an extremely useful tool, simple and fast to determine the degradation rate parameters of the compounds including pharmaceuticals and nutraceuticals. The shelf life needs to be defined by realist data. This is the first time that was used TG to determine the degradation reaction of probiotics.

O Mundo da Saúde

The microbiological contamination of pharmaceutical and cosmetic products represents a public hea... more The microbiological contamination of pharmaceutical and cosmetic products represents a public health problem because it can cause infections. Before purchasing a particular cosmetic, many consumers test it on their own skin, increasing the susceptibility to microbiological contaminations. We hypothesize that these lipstick testers available to pharmacy consumers represent a potential source of microbiological contamination. This study analyzed the microbiological quality of 30 lipstick samples, randomly collected, from different manufacturers, available to the consumers for trials in pharmacies in southern Brazil (fifteen samples were collected in Casca, five in Ciríaco and ten in Passo Fundo). The microbiological quality of the lipsticks was evaluated by: total count of viable bacteria and mold and yeast, and presence of pathogens Staphylococcus aureus and Pseudomonas aeruginosa. It was verified that amounts ranged from 1.0 x 10¹ to 1.9 x 105 CFU/g of viable bacteria and from 1.0 x 10¹ to 7.3 x 10³ CFU/g of molds and yeasts on the lipsticks. 54.33% and 40% of the samples were disapproved by the total count of viable bacteria and molds and yeasts, respectively. S. aureus, Aspergillus sp. and Cladosporium sp. were also found. Although the waxy composition of the lipsticks hinders microbial contamination this research reveals a large number of microorganisms. Our hypothesis that the lipsticks have a high microbiological load was confirmed. Opportunistic pathogenic microorganisms can become infectious agents in patients with compromised immune systems. The use of disposable applicators is proposed as an alternative way to avoid microbiological contamination of the cosmetic products.

Revista Verde de Agroecologia e Desenvolvimento Sustentável

Climate change can cause imbalances in plants. Baccharis trimera is a specie usually found in har... more Climate change can cause imbalances in plants. Baccharis trimera is a specie usually found in harsh conditions and has medicinal and agricultural properties. Thus, this study aimed to evaluate the biochemical and physiological responses of this plant under water restriction (WR) levels. B. trimera samples were identified and collected in Erechim/RS and propagated in a greenhouse. When acclimated, they were subjected to 0, 25, 75 and 100% WR, determined according to the water saturation in the soil by capillary action. After 30 days of treatment, the physiological responses: growth, and fresh and dry biomass; and the biochemical responses: activity of superoxide dismutase (SOD), guaiacol peroxidase (GP) and ascorbate peroxidase (APX) enzymes, proline, protein and hydrogen peroxide content, and lipid peroxidation, were determined. Data were submitted to regression analysis and Pearson correlation. The WR of 27.37%, on average, induced an increase in physiological parameters, but the r...