Fadile Zeyrek - Academia.edu (original) (raw)
Papers by Fadile Zeyrek
Mikrobiyoloji Bulteni, 2021
Akrep venomu, karmaşık protein içeriğiyle güçlü nörotoksik etkiler gösteren ve bu şekilde akrebin... more Akrep venomu, karmaşık protein içeriğiyle güçlü nörotoksik etkiler gösteren ve bu şekilde akrebin avını yakalayıp sindirmesinde önemli rol oynayan bir maddedir. Akrep sokan insanların vücudunda kısa süre içinde hayatı tehdit eden sistemik etkiler oluşabilmekte; deride kızarıklık, ağrı, yanma ve şişlik sonrası karın ağrısı, bulantı, kusma, çift görme görülebilmekte, hasta komaya girebilmektedir. Akrep venomunun antimikrobiyal etkiye sahip olduğu, içerdiği bazı bileşiklerin güçlü antibakteriyel ve antifungal etkiler gösterebildiği de bilinmektedir. Leishmania cinsi protozoonların neden olduğu; hastalarda deri, mukoza ve ölümcül iç organ tutulumuna yol açabilen leyşmanyazis, dünyada yaygın görülen vektör kökenli paraziter enfeksiyonlardandır. Leyşmanyazis tedavisinde yeni ilaçlara gereksinim vardır, zira son çalışmalarda yıllardır kullanılmakta olan antimon bileşiklerine dirençli Leishmania suşlarının geliştiği bildirilmektedir. Leishmania suşlarının, akrep venomunda da olduğu bilinen, antimikrobiyal etkili iyon kanalı inhibitörü peptitlere duyarlı olduğu bilinmektedir. Bu çalışmada ülkemizdeki Androctonus crassicauda türü akreplerden elde edilen özütlerdeki anti-leishmanial etkinliğin araştırılması amaçlanmıştır. Bu kapsamda, A.crassicauda venomu içeren özütün farklı dilüsyonları ile standart tedavide kullanılan meglumin antimonatın laboratuvarımızda sıvı azottan çıkarılarak önce NNN sonra RPMI-1640 besiyerinde çoğaltılan Leishmania tropica promastigotları üzerindeki etkileri in vitro koşullarda karşılaştırılarak ölçülmüştür. Ayrıca, özütün insan hücreleri için öldürücü olup olmadığı XTT hücre canlılık testi ile belirlenmiştir. Deneyler sonunda yarı-maksimum inhibisyon konsantrasyonu (IC 50) değeri, akrep venomu için 18.12 µg/ ml (17.33-18.94), meglumin antimonat için ise 8.411 µg/ml (7.922-8.927) olarak belirlenmiştir. Bu ön Kısa Bildiri/Short Communication
BMC gastroenterology, Jan 23, 2004
As anorexia and hypermetabolism are common in cirrhosis, leptin levels may be increased in this d... more As anorexia and hypermetabolism are common in cirrhosis, leptin levels may be increased in this disease. In this study, we investigated the relation between the severity of disease and serum leptin levels in post-hepatitis cirrhosis and the role of body composition, gender and viral aetiology of cirrhosis in this association. Thirty-five cases with post-hepatitis cirrhosis and 15 healthy controls were enrolled in this study. Body composition including body mass index, body fat percentage and body fat mass were determined. Serum leptin levels were assayed. Leptin levels were significantly higher among cirrhotic patients independent of sex compared to controls (p = 0.001). Female patients in both groups have had higher leptin levels than males (in cirrhotics p = 0.029, in controls p = 0.02). Cirrhotic patients in each of A, B and C subgroups according to the Child- Pugh classification revealed significantly different levels compared to controls (p = 0.046, p = 0.004, p = 0.0001, respe...
Urologia Internationalis, 2008
To evaluate the oxidative and antioxidative status in blood plasma after the induction of acute e... more To evaluate the oxidative and antioxidative status in blood plasma after the induction of acute epididymitis in an experimental rat model. Escherichia coli was inoculated into the ductus deferens of rats in the epididymitis group (n = 10), and saline, instead of bacteria, was injected into the saline group (n = 10). No inoculums were performed in control group (n = 10). The infection was confirmed by microbiological tests. As antioxidative parameters, the total peroxide (TP), indicative marker of lipoperoxidation and protein oxidation, and the total antioxidant response (TAR) were measured in blood samples. Acute epididymitis was demonstrated in all rats of the epididymitis group. The TP level increased significantly in the epididymitis group compared to the other two groups. The TAR level also increased significantly in the infected group (p < 0.05). Our results are concordant with the fact that the increased oxidative status caused by epididymitis was tolerated by the endogenous antioxidant defense system. Moreover, we suggest that the measurement of oxidative and antioxidative level may be useful in clinical practice to detect defective defense system and prevent possible complications.
Medical Journal of Trakya University, 2009
African Journal of …, 2010
... Full Length Research Paper. Comparison of culture media in the isolation and diagnosis of cut... more ... Full Length Research Paper. Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis. Ahmet Özbilgin 1 *, Fadile Zeyrek 2 , M. Emin Limoncu 3 , İpek Östan 3 , Tuba Tabak 1 , Kamil Aşar 1 , Hayriye Türkmen 4 and Ali A. Kilimcioğlu 1. ...
The American Journal of Medicine, 1992
ABSTRACT
American Journal of Tropical Medicine and Hygiene, 2010
The 200-kD merozoite surface protein of Plasmodium vivax (PvMSP-1) is one of the leading vaccine ... more The 200-kD merozoite surface protein of Plasmodium vivax (PvMSP-1) is one of the leading vaccine candidates against P. vivax malaria. However, the gene encoding PvMSP-1 (pvmsp1) is highly polymorphic and is a major obstacle to effective vaccine development. To further understand polymorphism in pvmsp1 , we obtained 30 full-length pvmsp1 sequences from southeastern Turkey. Comparative analysis of sequences from Turkey and other areas showed substantially limited polymorphism. Substitutions were found at 280 and 162 amino acid sites in samples from other regions and those from Turkey, respectively. Eight substitutions were unique to Turkey. In one of them, D/E at position 1706 in the C-terminal 19-kD region, the K/E change at 1709 was the only polymorphism previously known. Limited diversity was also observed in microsatellites. Data suggest a recent population bottleneck in Turkey that may have obscured a signature for balancing selection in the C-terminal 42-kD region, which was otherwise detectable in other areas.
The aim of this study was to examine the frequency and the relationship of H. pylori infection an... more The aim of this study was to examine the frequency and the relationship of H. pylori infection and giardiasis in children with recurrent abdominal pain. The study group included 98 patients and 88 healthy controls. Patients' sera were examined for anti-H. pylori specific IgG antibodies using H. pylori IgG ELISA. Analysis of stool samples was carried out by the H. pylori stool antigen (HpSA) enzyme immunoassay. For the diagnosis of giardiasis, all stool samples were examined by saline-Lugol and formalin-ethyl-acetate sedimentation methods. H. pylori was detected in 40 (49.0%) patients and 40 (45.5%) controls. G. intestinalis was detected in 30 (30.6%) patients and 18 (20.4%) controls. There was no significant difference in frequency between the groups in the distribution of H. pylori (p=0.6) and giardiasis (p=0.4). The frequency of the combination of H. pylori infection and giardiasis in the patient groups was 22.4% compared to 6.8% in the control groups and this result was stati...
Folia microbiologica, 2018
Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most fr... more Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP. Genomic DNA was extracted phenol-chloroform procedure. Samples were examined by using ITS 1 PCR followed by RFLP analysis. Our results indicated that two species, L. tropica (132 samples) and L. major (3 samples), are responsible for cutaneous leishmaniasis in Sanlıurfa. Our study is the first scientific study in which it is reported molecular analyses of cutaneous leishmaniasis cases caused by L. major in Sanliurfa...
BMC infectious diseases, Aug 11, 2017
Hepatitis A virus (HAV) is a food and water-borne virus causing clinical (mainly hepatitis) and s... more Hepatitis A virus (HAV) is a food and water-borne virus causing clinical (mainly hepatitis) and subclinical disease in humans. It is important to characterize circulating strains of HAV in order to prevent HAV infections using efficacious vaccines. The aim of this study was the detection and characterization of the circulating strains of HAV in Turkey by performing serology, RT-PCR, sequencing and phylogenetic analysis. In this study, 355 HAV suspected cases were analysed by ELISA for the presence of antibodies to HAV. RNA was extracted from 54 HAV IgM positive human sera. None of the suspect cases were vaccinated against HAV and they never received blood transfusions. Samples found positive by RT-PCR using primers targeting the VP1/VP2A junction and VP1/VP3 capsid region of HAV, were subjected to sequencing and phylogenetic analyses. IgM type antibodies to HAV were detected in 54 patients. Twenty one of them were students. The age of IgM positive cases was between 3 and 60 years. I...
International Journal of Food Microbiology, 2017
192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ov... more 192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in Sanliurfa city, Turkey in a year were collected to determine the Salmonella enterica subsp. enterica mosaic in Turkey. 161 Salmonella isolates represented 17 serotypes, 20 sequence types (STs) and 44 PFGE patterns (PTs). 3 serotypes, S. Enteritidis, S. Typhimurium and S. Kentucky, were recovered from three different hosts. The highest discriminatory power was obtained by PFGE (SID=0.945), followed by MLST (SID=0.902) and serotyping (SID=0.885) for all isolates. The prevalence of antimicrobial resistance genes (aadA1, aadA2, strA, strB, aphA1-Iab, blaTEM-1, blaPSE-1, tetA) was highly correlated with phenotypic profiles of aminoglycoside, ß-lactam and tetracycline groups (kappa >0.85). From our knowledge, this is the first study reporting spatial and temporal distribution of Salmonella species through phenotypic and genetic approaches over farm to fork chain in Turkey. Thus, our data provided further information for evolution, ecology and transmission of Salmonella in Turkey.
Turkiye Klinikleri Journal of Gastroenterohepatology, 2000
Journal of Medical Updates, 2013
Objective: To report isolation of L. major strains obtained from 18 Turkish autochthonous cutaneo... more Objective: To report isolation of L. major strains obtained from 18 Turkish autochthonous cutaneous leishmaniasis (CL) patients infected with Leishmania major between 2011 and 2014. Methods: Initial diagnosis relied on microscopy and culture in Enriched Medium, prepared by adding specific amounts of liver extract, protein and lipid sources to NNN medium. Promastigotes were then transferred to RPMI medium including 10% of fetal calf serum for mass culture. Species-specific real time PCR targeting ITS1 region of Leishmania spp. was performed using both lesion aspiration samples and cultured promastigotes. Two of 18 isolates were identified by isoenzyme analysis in the Leishmaniasis Reference Center in Montpellier, France. Each isolate was inoculated into the footpads of six mice to observe the pathogenicity of L. major. Developing lesions were observed and the thickening of footpads was measured weekly. Results: Melting curve analyses of 18 isolates showed a peak concordant with Leish...
![Research paper thumbnail of [Cutaneous Leishmaniasis Cases Caused by Leishmania infantum in Şanlıurfa Province, Turkey]](https://attachments.academia-assets.com/79294990/thumbnails/1.jpg)
](Mikrobiyoloji bulteni, 2020
Leishmaniases are a group of vector-borne diseases, and two clinical forms, visceral (VL) and cut... more Leishmaniases are a group of vector-borne diseases, and two clinical forms, visceral (VL) and cutaneous leishmaniasis (CL, Oriental sore), are seen in Turkey. While VL cases are recorded as 20-25 per year, CL cases are reported around 2000 per year, and nearly half of CL cases were recorded in Şanlıurfa province. Therefore, by knowing the epidemiology of the disease in Şanlıurfa province, it is possible to develop control measures and reduce the total number of cases across the country. Although Leishmania tropica is known as the main causative agent in Şanlıurfa, other Leishmania species have also been identified as a result of mass human movements in the last 10 years. In this study, we aimed to present the first CL cases caused by Leishmania infantum in Şanlıurfa. A total of 14 cases, which were admitted with the suspicion of CL and diagnosed as positive by direct microscopy and/or real-time ITS1-PCR using lesion aspiration samples are included in the study. Two or more smears we...
Turkiye Klinikleri Journal of Gastroenterohepatology, 2004
Le Infezioni in Medicina Rivista Periodica Di Eziologia Epidemiologia Diagnostica Clinica E Terapia Delle Patologie Infettive, Jun 1, 2007
Infective sacro-ileitis is due to common bacteria, 25% being tuberculosis and 10% brucellosis. Sl... more Infective sacro-ileitis is due to common bacteria, 25% being tuberculosis and 10% brucellosis. Slow progression characterizes joint tuberculosis, an uncommon variant of this disease. The onset is usually insidious, and early diagnosis requires a high index of clinical suspicion. We report two cases with tuberculous sacro-ileitis which initially mimicked brucellosis infiltration. Diagnosis of tuberculosis of the sacroiliac joint was established by fine-needle aspiration of joint and radiological imaging methods such as computerized tomography, magnetic resonance and three-phase bone scan. The current diagnosis and treatment of this condition is discussed based on these cases and a literature review.
Turkish Journal of Parasitology, 2016
Objective: The objective of this study was to determine the risk factors for and relationship amo... more Objective: The objective of this study was to determine the risk factors for and relationship among parasitic infections, growth retardation, and psychomotor developmental delays in children aged 6 years and below. Methods: This case-control study was performed in Şanlıurfa in southeastern Turkey between October and December 2007. Data were collected using a structured questionnaire, anthropometry, Ankara Development Screening Inventory, and laboratory analysis of stool specimens. Results: The most common parasite was Giardia intestinalis (42.53%) followed by Enterobius vermicularis (27.58%), Ascaris lumbricoides (18.39%), Hymenolepis nana (5.75%), Trichuris trichiura (3.45%), Escherichia coli (1.15%), and Blastocystis spp. (1.15%). Fifty-eight percent of all children were infected with intestinal parasites; 55.2% had only one parasite, whereas 44.8% had multiple parasites. The children infected with G. intestinalis and other intestinal parasites had significantly higher levels of growth retardation and psychomotor development delay than non-infected children. Children with parasitic infections had growth delay up to 2.9 times, general development delay up to 1.9 times, language-cognitive development delay up to 2.2 times, and fine motor development delay up to 2.9 times higher than children without any parasitic infections. However, no significant relationship among intestinal parasites, gross motor development, social-self skills, and development delay was identified. The education level of parents, poor economic situation, number of households, not washing hands, playing with soil, family history of parasitic infection were the significant risk factors for intestinal parasites. Conclusion: Our study indicates that the presence of either malnutrition or intestinal parasites may put a child in a high-risk group for developmental delays and growth retardation. Therefore, public health interventions can embrace nationwide deworming in children.
Current Eye Research, May 1, 2004
Taurolidine is a broad spectrum, non-antibiotic antimicrobial agent, not previously tested agains... more Taurolidine is a broad spectrum, non-antibiotic antimicrobial agent, not previously tested against infectious endophthalmitis. The efficacy of intravitreal taurolidine in the treatment of experimental Staphylococcus epidermidis endophthalmitis was evaluated and compared with vancomycin in a rabbit model. The right eyes of 34 albino rabbits were infected with an intravitreal inoculum of S. epidermidis (10(5) colony-forming units/0.1 ml). The right eyes of four rabbits (group 7) were not infected and served as uninfected controls. 24 hours after inoculation of bacteria the animals were divided into the following treatment groups: group 1 (7 rabbits) received intravitreal taurolidine at 24 hours and group 2 (7 rabbits) received at 48 hours. Group 3 (7 rabbits) received vancomycin at 24 hours and group 4 (7 rabbits) at 48 hours. Group 5 (3 rabbits) received polyvinylpyrrolidone at 24 hours and group 6 (3 rabbits) at 48 hours. Clinical scoring was performed at 24, 48 and 72 hours. At 72 hours post inoculation, vitreous samples were collected for quantitative microbiological studies and then, the eyes were enucleated for histopathological scorings. The clinical and histopathological examinations revealed significant amelioration of inflammation in eyes treated with taurolidine and vancomycin when compared with polyvinylpyrrolidone. The eyes treated with taurolidine also had significantly lower colony forming units than the eyes treated with polyvinylpyrrolidone and taurolidine rendered many eyes sterile. Taurolidine is expected to be a potential agent for treatment of S. epidermidis endophthalmitis.
Mikrobiyoloji Bulteni, 2021
Akrep venomu, karmaşık protein içeriğiyle güçlü nörotoksik etkiler gösteren ve bu şekilde akrebin... more Akrep venomu, karmaşık protein içeriğiyle güçlü nörotoksik etkiler gösteren ve bu şekilde akrebin avını yakalayıp sindirmesinde önemli rol oynayan bir maddedir. Akrep sokan insanların vücudunda kısa süre içinde hayatı tehdit eden sistemik etkiler oluşabilmekte; deride kızarıklık, ağrı, yanma ve şişlik sonrası karın ağrısı, bulantı, kusma, çift görme görülebilmekte, hasta komaya girebilmektedir. Akrep venomunun antimikrobiyal etkiye sahip olduğu, içerdiği bazı bileşiklerin güçlü antibakteriyel ve antifungal etkiler gösterebildiği de bilinmektedir. Leishmania cinsi protozoonların neden olduğu; hastalarda deri, mukoza ve ölümcül iç organ tutulumuna yol açabilen leyşmanyazis, dünyada yaygın görülen vektör kökenli paraziter enfeksiyonlardandır. Leyşmanyazis tedavisinde yeni ilaçlara gereksinim vardır, zira son çalışmalarda yıllardır kullanılmakta olan antimon bileşiklerine dirençli Leishmania suşlarının geliştiği bildirilmektedir. Leishmania suşlarının, akrep venomunda da olduğu bilinen, antimikrobiyal etkili iyon kanalı inhibitörü peptitlere duyarlı olduğu bilinmektedir. Bu çalışmada ülkemizdeki Androctonus crassicauda türü akreplerden elde edilen özütlerdeki anti-leishmanial etkinliğin araştırılması amaçlanmıştır. Bu kapsamda, A.crassicauda venomu içeren özütün farklı dilüsyonları ile standart tedavide kullanılan meglumin antimonatın laboratuvarımızda sıvı azottan çıkarılarak önce NNN sonra RPMI-1640 besiyerinde çoğaltılan Leishmania tropica promastigotları üzerindeki etkileri in vitro koşullarda karşılaştırılarak ölçülmüştür. Ayrıca, özütün insan hücreleri için öldürücü olup olmadığı XTT hücre canlılık testi ile belirlenmiştir. Deneyler sonunda yarı-maksimum inhibisyon konsantrasyonu (IC 50) değeri, akrep venomu için 18.12 µg/ ml (17.33-18.94), meglumin antimonat için ise 8.411 µg/ml (7.922-8.927) olarak belirlenmiştir. Bu ön Kısa Bildiri/Short Communication
BMC gastroenterology, Jan 23, 2004
As anorexia and hypermetabolism are common in cirrhosis, leptin levels may be increased in this d... more As anorexia and hypermetabolism are common in cirrhosis, leptin levels may be increased in this disease. In this study, we investigated the relation between the severity of disease and serum leptin levels in post-hepatitis cirrhosis and the role of body composition, gender and viral aetiology of cirrhosis in this association. Thirty-five cases with post-hepatitis cirrhosis and 15 healthy controls were enrolled in this study. Body composition including body mass index, body fat percentage and body fat mass were determined. Serum leptin levels were assayed. Leptin levels were significantly higher among cirrhotic patients independent of sex compared to controls (p = 0.001). Female patients in both groups have had higher leptin levels than males (in cirrhotics p = 0.029, in controls p = 0.02). Cirrhotic patients in each of A, B and C subgroups according to the Child- Pugh classification revealed significantly different levels compared to controls (p = 0.046, p = 0.004, p = 0.0001, respe...
Urologia Internationalis, 2008
To evaluate the oxidative and antioxidative status in blood plasma after the induction of acute e... more To evaluate the oxidative and antioxidative status in blood plasma after the induction of acute epididymitis in an experimental rat model. Escherichia coli was inoculated into the ductus deferens of rats in the epididymitis group (n = 10), and saline, instead of bacteria, was injected into the saline group (n = 10). No inoculums were performed in control group (n = 10). The infection was confirmed by microbiological tests. As antioxidative parameters, the total peroxide (TP), indicative marker of lipoperoxidation and protein oxidation, and the total antioxidant response (TAR) were measured in blood samples. Acute epididymitis was demonstrated in all rats of the epididymitis group. The TP level increased significantly in the epididymitis group compared to the other two groups. The TAR level also increased significantly in the infected group (p < 0.05). Our results are concordant with the fact that the increased oxidative status caused by epididymitis was tolerated by the endogenous antioxidant defense system. Moreover, we suggest that the measurement of oxidative and antioxidative level may be useful in clinical practice to detect defective defense system and prevent possible complications.
Medical Journal of Trakya University, 2009
African Journal of …, 2010
... Full Length Research Paper. Comparison of culture media in the isolation and diagnosis of cut... more ... Full Length Research Paper. Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis. Ahmet Özbilgin 1 *, Fadile Zeyrek 2 , M. Emin Limoncu 3 , İpek Östan 3 , Tuba Tabak 1 , Kamil Aşar 1 , Hayriye Türkmen 4 and Ali A. Kilimcioğlu 1. ...
The American Journal of Medicine, 1992
ABSTRACT
American Journal of Tropical Medicine and Hygiene, 2010
The 200-kD merozoite surface protein of Plasmodium vivax (PvMSP-1) is one of the leading vaccine ... more The 200-kD merozoite surface protein of Plasmodium vivax (PvMSP-1) is one of the leading vaccine candidates against P. vivax malaria. However, the gene encoding PvMSP-1 (pvmsp1) is highly polymorphic and is a major obstacle to effective vaccine development. To further understand polymorphism in pvmsp1 , we obtained 30 full-length pvmsp1 sequences from southeastern Turkey. Comparative analysis of sequences from Turkey and other areas showed substantially limited polymorphism. Substitutions were found at 280 and 162 amino acid sites in samples from other regions and those from Turkey, respectively. Eight substitutions were unique to Turkey. In one of them, D/E at position 1706 in the C-terminal 19-kD region, the K/E change at 1709 was the only polymorphism previously known. Limited diversity was also observed in microsatellites. Data suggest a recent population bottleneck in Turkey that may have obscured a signature for balancing selection in the C-terminal 42-kD region, which was otherwise detectable in other areas.
The aim of this study was to examine the frequency and the relationship of H. pylori infection an... more The aim of this study was to examine the frequency and the relationship of H. pylori infection and giardiasis in children with recurrent abdominal pain. The study group included 98 patients and 88 healthy controls. Patients' sera were examined for anti-H. pylori specific IgG antibodies using H. pylori IgG ELISA. Analysis of stool samples was carried out by the H. pylori stool antigen (HpSA) enzyme immunoassay. For the diagnosis of giardiasis, all stool samples were examined by saline-Lugol and formalin-ethyl-acetate sedimentation methods. H. pylori was detected in 40 (49.0%) patients and 40 (45.5%) controls. G. intestinalis was detected in 30 (30.6%) patients and 18 (20.4%) controls. There was no significant difference in frequency between the groups in the distribution of H. pylori (p=0.6) and giardiasis (p=0.4). The frequency of the combination of H. pylori infection and giardiasis in the patient groups was 22.4% compared to 6.8% in the control groups and this result was stati...
Folia microbiologica, 2018
Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most fr... more Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP. Genomic DNA was extracted phenol-chloroform procedure. Samples were examined by using ITS 1 PCR followed by RFLP analysis. Our results indicated that two species, L. tropica (132 samples) and L. major (3 samples), are responsible for cutaneous leishmaniasis in Sanlıurfa. Our study is the first scientific study in which it is reported molecular analyses of cutaneous leishmaniasis cases caused by L. major in Sanliurfa...
BMC infectious diseases, Aug 11, 2017
Hepatitis A virus (HAV) is a food and water-borne virus causing clinical (mainly hepatitis) and s... more Hepatitis A virus (HAV) is a food and water-borne virus causing clinical (mainly hepatitis) and subclinical disease in humans. It is important to characterize circulating strains of HAV in order to prevent HAV infections using efficacious vaccines. The aim of this study was the detection and characterization of the circulating strains of HAV in Turkey by performing serology, RT-PCR, sequencing and phylogenetic analysis. In this study, 355 HAV suspected cases were analysed by ELISA for the presence of antibodies to HAV. RNA was extracted from 54 HAV IgM positive human sera. None of the suspect cases were vaccinated against HAV and they never received blood transfusions. Samples found positive by RT-PCR using primers targeting the VP1/VP2A junction and VP1/VP3 capsid region of HAV, were subjected to sequencing and phylogenetic analyses. IgM type antibodies to HAV were detected in 54 patients. Twenty one of them were students. The age of IgM positive cases was between 3 and 60 years. I...
International Journal of Food Microbiology, 2017
192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ov... more 192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in Sanliurfa city, Turkey in a year were collected to determine the Salmonella enterica subsp. enterica mosaic in Turkey. 161 Salmonella isolates represented 17 serotypes, 20 sequence types (STs) and 44 PFGE patterns (PTs). 3 serotypes, S. Enteritidis, S. Typhimurium and S. Kentucky, were recovered from three different hosts. The highest discriminatory power was obtained by PFGE (SID=0.945), followed by MLST (SID=0.902) and serotyping (SID=0.885) for all isolates. The prevalence of antimicrobial resistance genes (aadA1, aadA2, strA, strB, aphA1-Iab, blaTEM-1, blaPSE-1, tetA) was highly correlated with phenotypic profiles of aminoglycoside, ß-lactam and tetracycline groups (kappa >0.85). From our knowledge, this is the first study reporting spatial and temporal distribution of Salmonella species through phenotypic and genetic approaches over farm to fork chain in Turkey. Thus, our data provided further information for evolution, ecology and transmission of Salmonella in Turkey.
Turkiye Klinikleri Journal of Gastroenterohepatology, 2000
Journal of Medical Updates, 2013
Objective: To report isolation of L. major strains obtained from 18 Turkish autochthonous cutaneo... more Objective: To report isolation of L. major strains obtained from 18 Turkish autochthonous cutaneous leishmaniasis (CL) patients infected with Leishmania major between 2011 and 2014. Methods: Initial diagnosis relied on microscopy and culture in Enriched Medium, prepared by adding specific amounts of liver extract, protein and lipid sources to NNN medium. Promastigotes were then transferred to RPMI medium including 10% of fetal calf serum for mass culture. Species-specific real time PCR targeting ITS1 region of Leishmania spp. was performed using both lesion aspiration samples and cultured promastigotes. Two of 18 isolates were identified by isoenzyme analysis in the Leishmaniasis Reference Center in Montpellier, France. Each isolate was inoculated into the footpads of six mice to observe the pathogenicity of L. major. Developing lesions were observed and the thickening of footpads was measured weekly. Results: Melting curve analyses of 18 isolates showed a peak concordant with Leish...
Mikrobiyoloji bulteni, 2020
Leishmaniases are a group of vector-borne diseases, and two clinical forms, visceral (VL) and cut... more Leishmaniases are a group of vector-borne diseases, and two clinical forms, visceral (VL) and cutaneous leishmaniasis (CL, Oriental sore), are seen in Turkey. While VL cases are recorded as 20-25 per year, CL cases are reported around 2000 per year, and nearly half of CL cases were recorded in Şanlıurfa province. Therefore, by knowing the epidemiology of the disease in Şanlıurfa province, it is possible to develop control measures and reduce the total number of cases across the country. Although Leishmania tropica is known as the main causative agent in Şanlıurfa, other Leishmania species have also been identified as a result of mass human movements in the last 10 years. In this study, we aimed to present the first CL cases caused by Leishmania infantum in Şanlıurfa. A total of 14 cases, which were admitted with the suspicion of CL and diagnosed as positive by direct microscopy and/or real-time ITS1-PCR using lesion aspiration samples are included in the study. Two or more smears we...
Turkiye Klinikleri Journal of Gastroenterohepatology, 2004
Le Infezioni in Medicina Rivista Periodica Di Eziologia Epidemiologia Diagnostica Clinica E Terapia Delle Patologie Infettive, Jun 1, 2007
Infective sacro-ileitis is due to common bacteria, 25% being tuberculosis and 10% brucellosis. Sl... more Infective sacro-ileitis is due to common bacteria, 25% being tuberculosis and 10% brucellosis. Slow progression characterizes joint tuberculosis, an uncommon variant of this disease. The onset is usually insidious, and early diagnosis requires a high index of clinical suspicion. We report two cases with tuberculous sacro-ileitis which initially mimicked brucellosis infiltration. Diagnosis of tuberculosis of the sacroiliac joint was established by fine-needle aspiration of joint and radiological imaging methods such as computerized tomography, magnetic resonance and three-phase bone scan. The current diagnosis and treatment of this condition is discussed based on these cases and a literature review.
Turkish Journal of Parasitology, 2016
Objective: The objective of this study was to determine the risk factors for and relationship amo... more Objective: The objective of this study was to determine the risk factors for and relationship among parasitic infections, growth retardation, and psychomotor developmental delays in children aged 6 years and below. Methods: This case-control study was performed in Şanlıurfa in southeastern Turkey between October and December 2007. Data were collected using a structured questionnaire, anthropometry, Ankara Development Screening Inventory, and laboratory analysis of stool specimens. Results: The most common parasite was Giardia intestinalis (42.53%) followed by Enterobius vermicularis (27.58%), Ascaris lumbricoides (18.39%), Hymenolepis nana (5.75%), Trichuris trichiura (3.45%), Escherichia coli (1.15%), and Blastocystis spp. (1.15%). Fifty-eight percent of all children were infected with intestinal parasites; 55.2% had only one parasite, whereas 44.8% had multiple parasites. The children infected with G. intestinalis and other intestinal parasites had significantly higher levels of growth retardation and psychomotor development delay than non-infected children. Children with parasitic infections had growth delay up to 2.9 times, general development delay up to 1.9 times, language-cognitive development delay up to 2.2 times, and fine motor development delay up to 2.9 times higher than children without any parasitic infections. However, no significant relationship among intestinal parasites, gross motor development, social-self skills, and development delay was identified. The education level of parents, poor economic situation, number of households, not washing hands, playing with soil, family history of parasitic infection were the significant risk factors for intestinal parasites. Conclusion: Our study indicates that the presence of either malnutrition or intestinal parasites may put a child in a high-risk group for developmental delays and growth retardation. Therefore, public health interventions can embrace nationwide deworming in children.
Current Eye Research, May 1, 2004
Taurolidine is a broad spectrum, non-antibiotic antimicrobial agent, not previously tested agains... more Taurolidine is a broad spectrum, non-antibiotic antimicrobial agent, not previously tested against infectious endophthalmitis. The efficacy of intravitreal taurolidine in the treatment of experimental Staphylococcus epidermidis endophthalmitis was evaluated and compared with vancomycin in a rabbit model. The right eyes of 34 albino rabbits were infected with an intravitreal inoculum of S. epidermidis (10(5) colony-forming units/0.1 ml). The right eyes of four rabbits (group 7) were not infected and served as uninfected controls. 24 hours after inoculation of bacteria the animals were divided into the following treatment groups: group 1 (7 rabbits) received intravitreal taurolidine at 24 hours and group 2 (7 rabbits) received at 48 hours. Group 3 (7 rabbits) received vancomycin at 24 hours and group 4 (7 rabbits) at 48 hours. Group 5 (3 rabbits) received polyvinylpyrrolidone at 24 hours and group 6 (3 rabbits) at 48 hours. Clinical scoring was performed at 24, 48 and 72 hours. At 72 hours post inoculation, vitreous samples were collected for quantitative microbiological studies and then, the eyes were enucleated for histopathological scorings. The clinical and histopathological examinations revealed significant amelioration of inflammation in eyes treated with taurolidine and vancomycin when compared with polyvinylpyrrolidone. The eyes treated with taurolidine also had significantly lower colony forming units than the eyes treated with polyvinylpyrrolidone and taurolidine rendered many eyes sterile. Taurolidine is expected to be a potential agent for treatment of S. epidermidis endophthalmitis.