Fatuma Nalubega - Academia.edu (original) (raw)
A Ugandan MSc microbiologist. I completed both my BSc and MSc from Algeria. Currently attached to the microbiology department of Makerere university college of health sciences, Uganda. I am interested in research, in the field of actinobacteria.
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Kwame Nkrumah University of Science and Technology, Kumasi Ghana
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Papers by Fatuma Nalubega
Research Square (Research Square), Jun 3, 2020
Journal of Clinical Microbiology, Dec 4, 2023
BMC Infectious Diseases, Jun 29, 2020
Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), cont... more Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the biofilm forming capability, presence of virulence genes and antimicrobial susceptibility pattern of Uropathogenic E. coli isolates in Uganda. Methods: This was a cross-sectional study carried in the Clinical Microbiology and Molecular biology laboratories at
مجلة الواحات للبحوث والدراسات
A new strain, designated TA4, was isolated from a sea water sample collected alongshore of Tipaza... more A new strain, designated TA4, was isolated from a sea water sample collected alongshore of Tipaza(36°35' North, 2°26'52.8 East)by a serial dilution method,and then spreaded on chitine-vitamines agar medium supplemented with an antifungal agent (actidioneat 50 mg/l). The taxonomic study based on the morphological, physiological and molecular criteria showed that this strain is closely related to Streptomyces olivaceusand S. pactum. The study of the antimicrobial activity on ISP2 solid medium showed that this strain presents a strong activity against two Gram positive bacteria (methicillin resistant Staphylococcus aureus639c and Bacillus subtilisATCC 6633). The kinetic of the production of antibacterial activity by wells diffusion method during 10 days in ISP2 liquid medium showed that incubation for 5 days gives the best activity. This result was confirmed by a second kinetic by diffusion method using paper discs impregnated with the extracts of ethyl acetate and dichloromethane. The second kinetic also showed that the highest antimicrobial activity was obtained with the ethyl acetate extract. The HPLC analysis of this extract showed the presence of two hydrophobic compounds and the the UV spectrum showed that they are aromatic or heterocyclicin nature.
مجلة الواحات للبحوث والدراسات
Revue ElWahat pour les Recherches et les Etudes
Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), cont... more Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the prevalence of biofilm formation among Uropathogenic E. coli clinical isolates, their relationship with antimicrobial susceptibility patterns, and Urovirulence genes. Methods: This was a cross-sectional study carried in the Clinical Microbiology and Molec...
Research Square (Research Square), Jun 3, 2020
Journal of Clinical Microbiology, Dec 4, 2023
BMC Infectious Diseases, Jun 29, 2020
Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), cont... more Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the biofilm forming capability, presence of virulence genes and antimicrobial susceptibility pattern of Uropathogenic E. coli isolates in Uganda. Methods: This was a cross-sectional study carried in the Clinical Microbiology and Molecular biology laboratories at
مجلة الواحات للبحوث والدراسات
A new strain, designated TA4, was isolated from a sea water sample collected alongshore of Tipaza... more A new strain, designated TA4, was isolated from a sea water sample collected alongshore of Tipaza(36°35' North, 2°26'52.8 East)by a serial dilution method,and then spreaded on chitine-vitamines agar medium supplemented with an antifungal agent (actidioneat 50 mg/l). The taxonomic study based on the morphological, physiological and molecular criteria showed that this strain is closely related to Streptomyces olivaceusand S. pactum. The study of the antimicrobial activity on ISP2 solid medium showed that this strain presents a strong activity against two Gram positive bacteria (methicillin resistant Staphylococcus aureus639c and Bacillus subtilisATCC 6633). The kinetic of the production of antibacterial activity by wells diffusion method during 10 days in ISP2 liquid medium showed that incubation for 5 days gives the best activity. This result was confirmed by a second kinetic by diffusion method using paper discs impregnated with the extracts of ethyl acetate and dichloromethane. The second kinetic also showed that the highest antimicrobial activity was obtained with the ethyl acetate extract. The HPLC analysis of this extract showed the presence of two hydrophobic compounds and the the UV spectrum showed that they are aromatic or heterocyclicin nature.
مجلة الواحات للبحوث والدراسات
Revue ElWahat pour les Recherches et les Etudes
Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), cont... more Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the prevalence of biofilm formation among Uropathogenic E. coli clinical isolates, their relationship with antimicrobial susceptibility patterns, and Urovirulence genes. Methods: This was a cross-sectional study carried in the Clinical Microbiology and Molec...