Fernanda Coelho - Academia.edu (original) (raw)

Papers by Fernanda Coelho

Journal of Basic and Applied Pharmaceutical Sciencies, 2017

Introducao: Os cimentos de fosfato de calcio (CFCs) tem sido amplamente explorados como enxerto s... more Introducao: Os cimentos de fosfato de calcio (CFCs) tem sido amplamente explorados como enxerto sintetico alternativo por poderem ser moldados ou injetados para serem utilizados como preenchimento de defeito osseo de modo a adaptarem-se a forma das superficies. Alem disso, a silica mesoporosa tem sido amplamente utilizada para a reparacao ossea devido a sua elevada area superficial e porosidade, alem de suas excelentes propriedades biologicas, como compatibilidade e bioatividade, as quais permitem que essas particulas sejam usadas como potenciais portadores de moleculas biologicas ativas. Objetivo: O objetivo foi avaliar a citotoxicidade (ensaio XTT e sobrevivencia clonogenica- SC), genotoxicidade (ensaio cometa - EC) e mutagenicidade (teste de micronucleo - MN) de cimento injetavel de fosfato de calcio (CaP), CaP associado a silica mesoporosa (CaPSi) e CaP associado a silica mesoporosa funcionalizada com peptideo de crescimento osteogenico (CaPSi-OGP). Metodologia: Foi realizado tr...

Journal of Basic and Applied Pharmaceutical Sciencies, 2015

Introducao: O poli acido latico-co-acido glicolico (PLGA) e um copolimero biodegradavel e bioreab... more Introducao: O poli acido latico-co-acido glicolico (PLGA) e um copolimero biodegradavel e bioreabsorvivel que tem sido estudado para aplicacao em sistemas de liberacao controlada de drogas e como suporte para celulas para aplicacoes medicas e odontologicas. Biomateriais osteocondutores vem apresentando resultados promissores na estimulacao de crescimento osseo em casos de defeitos criticos de fraturas, pois possibilitam maior migracao das celulas para formacao do tecido osseo. Para que essas celulas tenham uma aderencia ao tecido, sao usados polimeros bioabsorviveis, onde essas celulas reparadoras fixam-se ate a formacao do tecido. Neste contexto, a empresa Bionnovation Produtos Biomedicos Ltda desenvolveu materiais a base de Hidroxiapatita® (HA) e Bonefill® (BO – osso bovino inorgânico) com adicao de PLGA para estimulacao ossea. Objetivo: O objetivo do presente trabalho e avaliar a genotoxicidade e mutagenicidade dos materiais Bonefill® com adicao de PLGA (BO/PLGA) e Hidroxiapatita...

This article describes the use of practice in the area of Management Project, Information Technol... more This article describes the use of practice in the area of Management Project, Information Technology and Performance Management to deployment an extension project. The objective of this project was to capacitate community members to work outside the clothing sector through a continuous training, which included some concepts relevant to Industrial Engineering as PCP and Quality Engineering. Elements considered as a basis for promoting the social and digital inclusion of individuals in industry sectors. The use of practices has improved the management, implementation and evaluation of activities under the project extension.

Journal of Basic and Applied Pharmaceutical Sciencies, 2016

Introducao: O polimero poli (e-caprolactona) – PCL possui excelentes propriedades mecânicas. Um d... more Introducao: O polimero poli (e-caprolactona) – PCL possui excelentes propriedades mecânicas. Um dos metodos para melhorar sua biocompatibilidade e tempo de reabsorcao e a adicao de fases inorgânicas. Alem disso, uma tendencia no desenvolvimento de compositos polimericos tem sido torna-los luminescentes para facilitar a visualizacao dos mesmos em diagnosticos por imagens ou para uso em terapia fotodinâmica. Dentre os elementos terras-raras, o erbio e iterbio apresentam a propriedade de luminescencia. Objetivo: O objetivo deste trabalho foi avaliar o potencial citotoxico (XTT e Sobrevivencia Clonogenica - SC), genotoxico (Ensaio Cometa- EC) e mutagenico (Teste do Micronucleo- MN) e do PCL puro e associado a oxiapatita dopada com Erbio e Iterbio (P+E+I) em celulas CHO-K1. Metodologia: Foi realizado tratamento das celulas por 24h com eluatos confeccionados a partir dos materiais, seguindo a ISO 10993:12 em diferentes concentracoes (100%, 75%, 50%, 25%). Como controles positivos, foram u...

Biological Conservation, 2021

Clinical Oral Investigations, 2021

Objective This study evaluated the metabolic activity of hydro-carbon-oxo-borate complex (HCOBc) ... more Objective This study evaluated the metabolic activity of hydro-carbon-oxo-borate complex (HCOBc) on a multispecies subgingival biofilm as well as its effects on cytotoxicity. Materials and methods The subgingival biofilm with 32 species related to periodontitis was formed in the Calgary Biofilm Device (CBD) for 7 days. Two different therapeutic schemes were adopted: (1) treatment with HCOBc, 0.12% chlorhexidine (CHX), and negative control group (without treatment) from day 3 until day 6, two times a day for 1 min each time, totaling 8 treatments and (2) a 24-h treatment on a biofilm grown for 6 days. After 7 days of formation, biofilm metabolic activity was determined by colorimetry assay, and bacterial counts and proportions of complexes were determined by DNA-DNA hybridization. Both substances’ cytotoxicity was evaluated by cell viability (XTT assay) and clonogenic survival assay on ovary epithelial CHO-K1 cells and an osteoblast precursor from calvaria MC3T3-E1 cells. Results The...

The novel coronavirus disease (COVID-19) is an emerging infectious disease caused by SARS-CoV-2, ... more The novel coronavirus disease (COVID-19) is an emerging infectious disease caused by SARS-CoV-2, which began as an outbreak in Wuhan, China and spread rapidly throughout the globe. Although the majority of infections are mild, severe and critical COVID-19 patients face deterioration of respiratory function, and may also present extrapulmonary manifestations, mostly affecting the kidney, digestive tract, heart and nervous system. Here, we prospectively evaluated the presence of SARS-CoV-2 genetic material by RT-PCR in urine samples obtained from critical care COVID-19 patients. In 51 patients included, we found higher serum creatinine levels, a longer hospital stay and a more frequent dialysis need in urine-positive patients. These findings could suggest that, in predisposed patients, a direct viral cytopathic effect may contribute to a more severe disease phenotype.

Nephrology Dialysis Transplantation, 2020

Background and Aims Evaluation and management of fluid balance are a key challenge in caring for ... more Background and Aims Evaluation and management of fluid balance are a key challenge in caring for the critically ill requiring renal replacement therapy. As intradialytic hypotension is a common complication and can be associated ultrafiltration rate. Usually the nephrologist and critical care physician have relied in their subjective clinical judgment and clinical data to calculate ultrafiltration. The aim of this study was to assess the prediction of clinical judgment of fluid overload and other clinical and dialysis data as predictors of hemodynamic tolerance to intermittent dialysis. Method This prospective observational single-center study was performed in critically ill patients with acute kidney injury undergoing intermittent hemodialysis. Before hemodialysis, clinical judgement of Hypervolemia from nephrologists and intensivists were assessed and posterior-anterior views of chest-X-rays done within three hour before of beginning of dialysis were collected for measurement of v...

Brazilian Journal of Pharmaceutical Sciences, 2018

Bone substitutes based on hydroxyapatite (HA) and Bonefill ® (BO-inorganic bovine bone) associate... more Bone substitutes based on hydroxyapatite (HA) and Bonefill ® (BO-inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill ®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN), micronucleus (FMN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) were analysed by cytokinesis-block micronucleus assay (CBMN assay). Results showed no statistical difference in cell viability compared with negative control (NC) The eluates did not promote delayed cytotoxicity whereas the surviving fraction rate for cultured cells was similar to NC. Furthermore, no genotoxicity or mutagenicity effects were observed for cultured cells with the Bonefill/PLGA and HA/PLGA eluates. In conclusion, the negative cytotoxicity, genotoxicity and mutagenicity results indicate that these bone substitutes presented interesting preliminary results as potential biomaterials for bone repair.

Toxicology in Vitro, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Journal of the Brazilian Chemical Society, 2020

Upconversion nanoparticles (UCNPs) are suitable materials for bioapplications due to their abilit... more Upconversion nanoparticles (UCNPs) are suitable materials for bioapplications due to their ability to emit visible light under near infrared (NIR) excitation, in the biological transparency range. Polycaprolactone(PCL)-based scaffolds are widely used in tissue engineering in combination with inorganic compounds to improve bioactivity and osteoconductive properties. This work proposes a 3D printed composite scaffold with upconversion property aiming at biomedical applications in therapy-stimulated bone repair and photodynamic therapy (PDT). The system combines PCL polymer, UCNPs-apatite and a PDT photosensitizer. Thermal and rheological behaviors of the composite were similar to pure PCL polymer. Mechanical properties were improved by adding UCNPs-apatite. The 3D printable composite presented upconversion property and potential for PDT application, which was demonstrated by singlet oxygen generation under 980 nm excitation. Cytotoxicity, genotoxicity and mutagenicity assays indicated no toxicological effects at low concentrations of rare earth elements. Taken together, a potential multifunctional material is proposed for biomedical applications.

Contact Lens and Anterior Eye, 2019

In a previous study, cyclodextrins (CD) and medications such as ciprofloxacin (CP) and diclofenac... more In a previous study, cyclodextrins (CD) and medications such as ciprofloxacin (CP) and diclofenac sodium (DS) were incorporated into the lenses to provide therapeutic properties and control drug release. However, significant opacity was seen in the material inherent to cellulose. In order to achieve full material transparency, the lenses were coated with an organic-inorganic hybrid compound containing aluminum alkoxide and glycidoxypropyltrimethoxysilane (GPTS)(H), or chitosan (Q) nanoparticles. This study evaluated the toxicity of these contact lenses to ensure the safety of these materials for future availability to the medical device industry. Lenses composed of BC and coated with either GPTS (H) or chitosan (Q), incorporating ciclodextrin (CD) to release diclofenac sodium (DS) or ciprofloxacin (CP), were submitted to cytotoxicity assays (XTT and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesisblocked micronucleus assay) directly in cell culture. Statistical analyses were performed using the Tukey and Dunnett or Kruskal-Wallis and Dunn tests. All of the nanoparticles used in the lense coatings did not show cytotoxic effects by the XTT test (p > 0.05; Dunnett). Only materials associated with diclofenac sodium (BC-H-CD-DS and BC-Q-CD-DS) presented significantly different survival fractions compared to negative control (p < 0.001; Dunnett). Genotoxicity evaluation revealed a genotoxic effect in BC-H-CD-DS (p < 0.05; Dunn). All tested lenses did not present any mutagenic effect. These results indicate that improvements in DS incorporation are needed to eliminate toxicity. We demonstrated promising results in the safety of employing BC lenses functionalized with a drug delivery system permitting the bioavailability of ophthalmic drugs. Further studies utilizing other specific tests, such as corneal lineage are required before safe and efficient ophthalmologic use.

European journal of medicinal chemistry, Jan 28, 2018

N-oxide derivatives 5(a-b), 8(a-b), and 11(a-c) were designed, synthesized and evaluated in vitro... more N-oxide derivatives 5(a-b), 8(a-b), and 11(a-c) were designed, synthesized and evaluated in vitro and in vivo as potential drugs that are able to ameliorate sickle cell disease (SCD) symptoms. All of the compounds demonstrated the capacity to releasing nitric oxide at different levels ranging from 0.8 to 30.1%, in vivo analgesic activity and ability to reduce TNF-α levels in the supernatants of monocyte cultures. The most active compound (8b) protected 50.1% against acetic acid-induced abdominal constrictions, while dipyrone, which was used as a control only protected 35%. Compounds 8a and 8b inhibited ADP-induced platelet aggregation by 84% and 76.1%, respectively. Both compounds increased γ-globin in K562 cells at 100 μM. The mechanisms involved in the γ-globin increase are related to the acetylation of histones H3 and H4 that is induced by these compounds. In vitro, the most promising compound (8b) was not cytotoxic, mutagenic and genotoxic.

Dementia & Neuropsychologia, 2013

Annually, 700,000 people are hospitalized with brain injury acquired after traumatic brain injury... more Annually, 700,000 people are hospitalized with brain injury acquired after traumatic brain injury (TBI) in Brazil. Objective: We aim to review the basic concepts related to TBI, and the most common Behavioral and Psychological Symptoms of Dementia (BPSD) findings in moderate and severe TBI survivors. We also discussed our strategies used to manage such patients in the post-acute period. Methods: Fifteen TBI outpatients followed at the Center for Cognitive Rehabilitation Post-TBI of the Clinicas Hospital of the University of São Paulo were submitted to a neurological, neuropsychological, speech and occupational therapy evaluation, including the Mini-Mental State Examination. Rehabilitation strategies will then be developed, together with the interdisciplinary team, for each patient individually. Where necessary, the pharmacological approach will be adopted. Results: Our study will discuss options of pharmacologic treatment choices for cognitive, behavioral, or affective disorders fol...

Planta medica, Jan 29, 2016

The leaves of Echinodorus grandiflorus are traditionally used in Brazil to treat several inflamma... more The leaves of Echinodorus grandiflorus are traditionally used in Brazil to treat several inflammatory conditions, including arthritis. This study aimed to investigate the antiarthritis activity of the 70 % ethanol extract of E. grandiflorus leaves and a standardized flavonoid-rich fraction in an antigen-induced arthritis model in mice. Previously immunized mice were treated per os with saline (control group), 70 % ethanol extract (100-1000 mg/kg), or a flavonoid-rich fraction (0.7-7.2 mg/kg) 40 minutes before and 3 and 6 hours after the challenge with antigen into the knee joint. The administration of the 70 % ethanol extract and flavonoid-rich fraction to mice significantly reduced neutrophil recruitment to the joint cavity and in periarticular tissue. The levels of chemokine (C-X-C motif) ligand 1, tumor necrosis factor-α, and interleukin-1β quantified by the enzyme-linked immunosorbent assay (ELISA) in the periarticular tissue were also diminished in mice treated with the 70 % et...

Dementia & Neuropsychologia, 2014

Traumatic brain injury (TBI) is a major cause of lifelong disability and death worldwide. Sport-r... more Traumatic brain injury (TBI) is a major cause of lifelong disability and death worldwide. Sport-related traumatic brain injury is an important public health concern. The purpose of this review was to highlight the importance of sport-related concussions. Concussion refers to a transient alteration in consciousness induced by external biomechanical forces transmitted directly or indirectly to the brain. It is a common, although most likely underreported, condition. Contact sports such as American football, rugby, soccer, boxing, basketball and hockey are associated with a relatively high prevalence of concussion. Various factors may be associated with a greater risk of sport-related concussion, such as age, sex, sport played, level of sport played and equipment used. Physical complaints (headache, fatigue, dizziness), behavioral changes (depression, anxiety, irritability) and cognitive impairment are very common after a concussion. The risk of premature return to activities includes ...

Neuropsychiatric Disease and Treatment, 2014