Francinete Campos - Academia.edu (original) (raw)

Papers by Francinete Campos

PloS one, 2018

The intense use of herbicides for weed control in agriculture causes selection pressure on soil m... more The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbi...

Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE The leaves of Eugenia biflora (Myrtaceae) are traditionally used b... more ETHNOPHARMACOLOGICAL RELEVANCE The leaves of Eugenia biflora (Myrtaceae) are traditionally used by Amazonian populations for the control of diabetes. However, their chemical composition has not yet been described and pharmacological evidence has not been reported. OBJECTIVE This study aimed to identify the chemical constituents and evaluate the hypoglycemic and toxic effect of the dry extract of the E. biflora leaves (DEEB). MATERIALS AND METHODS DEEB, obtained by infusion, was analyzed using LC-HRMS and NMR, whose the catechin flavonoid was quantified using NMR. The antidiabetic effect of DEEB was evaluated according to its inhibition of the enzymes α-amylase and α-glucosidase, as well as the content of total phenols, free radical scavengingand antiglycation activities, and its in vitro cell viability. Oral maltose tolerance and chronic multiple dose tests (28 days) in streptozotocin-induced diabetic mice (STZ) were performed. The hypoglycemic effect and toxicity of this extract were evaluated in the multiple dose assay. Biochemical parameters, hemolysis, and levels of the thiobarbituric acid reactive species in the liver were investigated and histopathological analyses of the kidneys and liver were performed. RESULTS Eight phenolic compounds were identified, with catechin (15.5 ± 1.7 mg g-1) being the majority compound and a possible chemical marker of DEEB. The extract showed inhibition activity of the enzyme α-glucosidase. Chronic administration of DEEB (50 mg/kg of body weight) reduced glucose levels in diabetic animals, similar to acarbose; however, DEEB (100 and 200 mg/kg bw) caused premature death of mice by D22 of the treatment. Our data indicate that one of the mechanisms of toxicity in DEEB may be related to the aggravation of oxidative stress in the liver. This histopathological study indicated that DEEB failed to minimize the progression of the toxicity of diabetes caused by STZ. CONCLUSIONS This study demonstrated the hypoglycemic potential of E. biflora leaves. However, the prolonged use of this tea can be harmful to its users due to its considerable toxicity, which needs to be better investigated.

International journal of child health and human development, 2012

IntroductionAcetonitrile, also known as cyanomethane, methylcyanide, ethanenitrile, methanecarbon... more IntroductionAcetonitrile, also known as cyanomethane, methylcyanide, ethanenitrile, methanecarbonitrile, ethylnitrile and methylcyanure, is a volatile colorless liquid with an odor similar to ether (1). It occurs naturally or is obtained as a by-product of acrylonitrile, an important starting material in the manufacture of plastics and synthetic rubber (1-3).In manufacturing operations, acetonitrile has been utilized as an intermediate substance to produce other organic chemicals. Due to its unique chemical properties such as its polarity, miscibility with water, low boiling point and low UV cut-off, acetonitrile is considered a versatile solvent with ample uses in analytical applications. It is also used in extractive processes, distillation in the petrochemical industry and the development and manufacturing of cosmetics, pharmaceutical and agricultural products, among other uses (1,4-8).In recent years, the utilization of acetonitrile has increased substantially, with the pharmace...

Journal of Pharmaceutical and Biomedical Analysis, 2020

Baccharis trimera is a species recognized by health agencies and recommended by the Brazilian Pha... more Baccharis trimera is a species recognized by health agencies and recommended by the Brazilian Pharmacopoeia by having medicinal properties. In this work, HR-MAS NMR spectroscopy combined with chemometric tools, such as Principal Components Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA) and Soft Independent Modeling by Class Analogy (SIMCA), were used to evaluate the quality control and authenticity of commercial samples of Baccharis, as well as to discriminate B. trimera samples from other species of the Caulopterae section (B. articulata, B. trimera, B. junciformis, B. milleflora, and B. myriocephala). The high morphological similarity of these species makes it difficult for their identifications and discriminations, even by taxonomists. Different PCA pre-processing (autoscaling, Pareto scaling, and mean centering) allowed to discriminate B. trimera and B. myriocephala from the other species, mainly due to the presence of carquejyl acetate, indicated their chemical similarity. The 1 H HR-MAS NMR spectral profile offers the possibility of tracking not only the chemical markers, such as the presence of carquejyl acetate, which can also be helpful in the B. trimera authentication/identification. The application of classification methods in standard samples revealed that PLS-DA models showed better performance on the calibration and validation sets than SIMCA model. However, PLS-DA and SIMCA applied to commercial samples showed that none of the commercial samples were classified as B. trimera, which suggested the lack of strict quality control regarding these products. The methodology developed in the present work might contribute to chemotaxonomy of the genus Baccharis.

Food Research International, 2019

Species of Eugenia have been used as an antidiabetic natural source. Chemical, antioxidant and an... more Species of Eugenia have been used as an antidiabetic natural source. Chemical, antioxidant and antiglycant screening of extracts from pedra-ume caá (Eugenia punicifolia) fruits were performed. 1 H NMR assisted by non-supervised chemometric methods were employed for the evaluation of the chemical profiles which were distinguished according to the color of fruit maturation stages, as well as for pulp and seed fruit. Furthermore, 1 H NMR fingerprint analysis of the crude extract allowed the identification of quercitrin and myricitrin, beside other nine compounds. The extracts of the yellow (YP) and green (GP) pulps presented higher antiglycant and antioxidant activities. Fresh juice from E. punicifolia was encapsulated in microcapsules produced with dextrose equivalent (DE) of 10, 20 or 30 as wall materials for the maintainment of their antioxidant and antiglycant properties. The more efficient retention of the bioactive compounds was found using the DE30. The Encapsulation Efficiency (EE) and the Retention Efficiency (RE) of this system was found around 89.7% and 97.6%, respectively. In addition, NMR spectra revealed the presence of flavonoids O-glycosylated (quercitrin and myricitrin) which might be related to the antiglycant and antioxidant activities. The YP presented larger content of quercitrin (117.6±0.4 mg per each 100 g of fresh fruit). Therefore, pedra-ume caá should be employed as an alternative nutraceutical source, as well as intherapeutic pourposes.

Chemistry & Biodiversity, 2019

The aim of this study was to evaluate the chemical, antioxidant, and antimicrobial activity of th... more The aim of this study was to evaluate the chemical, antioxidant, and antimicrobial activity of the essential oils as well as the anatomy of the aerial parts from Baccharis aracatubaensis, Baccharis burchellii, and Baccharis organensis owing to the therapeutic potential of Baccharis. The volatile constituents were analyzed using GC/MS, the antioxidant activity was evaluated by oxygen radical absorbance capacity (ORAC FL) and DPPH assays, and the antimicrobial activity by a microdilution technique. Of the 56 compounds identified, only seven (β-caryophyllene, γ-muurolene, bicyclogermacrene, β-germacrene, spathulenol, τ-muurolol, and α-cadinol) were common in the three specimens studied. Of these, γ-muurolene was found abundantly in B. aracatubaensis, while bicyclogermacrene was abundant in B. burchellii and B. organensis. The essential oils exhibited antioxidant activity in the ORAC FL (> 500.0 μmol TE g À 1) and DPPH assays. However, they did not exhibit any antimicrobial activity. Secretory ducts and flagelliform glandular trichomes were observed in the anatomical study of all the Baccharis species studied.

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Oxidative medicine and cellular longevity, 2018

Pequi () is an endemic species from Brazilian Cerrado, and their fruits are widely used in region... more Pequi () is an endemic species from Brazilian Cerrado, and their fruits are widely used in regional cuisine. In this work, a crude hydroalcoholic extract (CHE) of leaves and its resulting fractions in hexane (HF), chloroform (CF), ethyl acetate (EAF), and butanol (BF) were investigated for their antioxidant properties and anticholinesterase activities. The antioxidant properties were evaluated by free radical scavenging and electroanalytical assays, which were further correlated with the total phenolic content and LC-MS results. The acetylcholinesterase and butyrylcholinesterase inhibitory activities were examined using Ellman's colorimetric method. The LC-MS analysis of EAF revealed the presence of gallic acid and quercetin. CHE and its fractions, EAF and BF, showed anticholinesterase and antioxidant activities, suggesting the association of both effects with the phenolic content. In addition, behavioral tests performed with CHE (10, 100, and 300 mg/kg) showed that it prevented...

Behavioural brain research, Jan 4, 2018

Parkinson's disease is a chronic neurodegenerative disorder characterized by cardinal motor f... more Parkinson's disease is a chronic neurodegenerative disorder characterized by cardinal motor features, such as bradykinesia, but also vocal deficits (e.g. difficulties to articulate words and to keep the tone of voice) and depression. In the present study, rats with bilateral 6-hydroxydopamine lesion of the substantia nigra pars compacta were evaluated for changes in the emission of 50-kHz ultrasonic vocalizations, gait impairment (catwalk test), and depressive-like behaviour (sucrose preference test).Furthermore, we evaluated the effect of repeated treatment (28 days) with ketamine (5, 10, and 15 mg/kg, ip, once per week) or imipramine (15 mg/kg, ip, daily). The lesion had prominent effects on the production of 50-kHz ultrasonic vocalizations(reduced call numbers, call durations, total calling time, and increased latency to start calling), led to gait impairment (increased run duration and stand of right forelimb) and induced anhedonia (reduced sucrose preference). Also, signifi...

Food Chemistry, 2018

1 H HR-MAS NMR spectroscopy was used to track the metabolic changes throughout the whole developm... more 1 H HR-MAS NMR spectroscopy was used to track the metabolic changes throughout the whole development of astringent ('Giombo') and non-astringent ('Fuyu') cultivars of persimmon (Diospyros kaki). The NMR data revealed the low concentration of amino acids (threonine, alanine, citrulline and GABA) and organic acids (malic acid). In addition, the signals of carbohydrates (sucrose, glucose and fructose) seemed to play the most important role in the fruit development. In both cultivars, the growth was characterized by fluctuating sucrose concentration along with a constant increase in both glucose and fructose. In the initial growth stage, the polyphenol composition was quite different between the cultivars. Gallic acid was detected throughout the growth of 'Giombo', while for 'Fuyu', signals of polyphenols disappeared over time. Additional multivariate analysis suggested that these cultivars share many metabolic similarities during development. These findings might help the comprehension of fruit development, which in turn, impacts the quality of the fruits.

Journal of the Brazilian Chemical Society, 2016

The paper describes the isolation of ergosterol peroxide, monocerin, annularin I and annularin J ... more The paper describes the isolation of ergosterol peroxide, monocerin, annularin I and annularin J compounds of Exserohilum rostratum biomass extracts. Compounds were isolated by silica gel column chromatography in polarity gradient and the structures were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) methods. Monocerin, annularin I and annularin J compounds were tested against Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis bacteria and showed moderate activity. Annularin I and annularin J compounds are new natural products.

Revista Brasileira de Farmacognosia, 2014

Baccharis glaziovii Baker, Asteraceae, also known as carqueja or carqueja-arbustinho, is a native... more Baccharis glaziovii Baker, Asteraceae, also known as carqueja or carqueja-arbustinho, is a native shrub of Brazil that reaches 0.5-2.5 m in height. It is a dioecious species that blossoms from September to December. This species has cladodes, which are winged stems that belong to the "carquejas" and are widely used indiscriminately by the population due to their gastric and diuretic properties. Carquejas are included in section Caulopterae and are difficult to identify even for taxonomists or Baccharis specialists. In the present study, a morpho-anatomical (cladodes and leaves) analysis of the medicinal plant was undertaken to improve its identification and add to the knowledge of section Caulopterae. Fragments of cladodes and leaves were collected and prepared in accordance with standard optical and scanning electron microscopy techniques. The morpho-anatomical characteristics found in B. glaziovii, include three-winged stems showing wings in a regular arrangement around the stem axis, short and petiolate leaves, flagelliform and simple non-glandular trichomes, concave-convex midrib, petioles with a concave shape and a slight projection on the adaxial face and convex with three projections on the abaxial surface, and calcium oxalate crystals in the form of raphides, styloids and pyramidal in the perimedullary region of the cladode, when evaluated as a whole, provide support for the quality control.

Revista Brasileira de Farmacognosia, 2010

No Brasil as espécies de Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae,... more No Brasil as espécies de Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae, popularmente conhecidas como guaco, são amplamente utilizadas com evidências pré-clínicas para o tratamento de doenças onde estejam envolvidas suas ações broncodilatadoras, antialérgicas, antiasmáticas, anti-inflamatórias, antiulcerogênicas e relaxantes da musculatura lisa. Em virtude das propriedades terapêuticas atribuídas as essas espécies, ambas foram oficializadas na farmacopéia brasileira, e por serem bastante semelhantes, dificilmente são diferenciadas. Com o objetivo de contribuir com novas pesquisas, a presente revisão descreve, de forma sistematizada, sobre os aspectos farmacológicos, toxicológicos, químicos, agronômicos, morfoanatômicos, genéticos e o uso nos programas de fitoterapia do Brasil destas espécies. As principais diferenças e semelhanças entre as duas espécies são evidenciadas através da comparação das informações baseadas na literatura específica. Unitermos: Asteraceae, Mikania glomerata, Mikania laevigata, guaco, plantas medicinais. ABSTRACT: "Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae: agronomic, genetic, anatomical, chemical, pharmacological, toxicological studies and its use in herbal therapy programs in Brazil." In Brazil the species of Mikania glomerata Spreng. and M. laevigata Sch. Bip. ex Baker, Asteraceae popularly known as guaco, are widely used with preclinical evidence for the treatment of diseases whose actions involve bronchodilators, antiallergic, anti-asthmatic, anti-inflammatory, antiulcerogenic and smooth muscles relaxant. Due to the therapeutic properties attributed to these species, both were included in the official Brazilian Pharmacopoeia, and because of their similarity, they are hardly differentiated. In order to contribute to the new research, this review describes their several aspects: pharmacology, toxicology, chemical, agricultural, anatomical, genetics and the use in herbal medicine programs in Brazil, in a systematic way. The main differences and similarities between the two species are evidenced by comparing the information based on literature.

Journal of the Brazilian Chemical Society, 2009

A fração diclorometano obtida pelo particionamento do extrato hidroalcoólico obtido das folhas e ... more A fração diclorometano obtida pelo particionamento do extrato hidroalcoólico obtido das folhas e flores de Vernonia scorpioides (Asteraceae) resultou no isolamento e caracterização de um novo poliacetileno, 5-octa-2,4,6-triinil-furan-2(5H)-ona. A determinação estrutural foi baseada em dados de espectrometria de Ressonância Magnética Nuclear mono e bidimensionais, de Massas e de Infravermelho. Foi investigado o potencial antitumoral e genotóxico do novo poliacetileno e os resultados encontrados sugerem genotoxicidade e citotoxicidade, onde a morte celular ocorreu via apoptose, envolvendo um mecanismo dependente de caspase. The dichloromethane fraction obtained from hydroalcoholic crude extract of leaves and flowers of Vernonia scorpioides (Asteraceae) was investigated, resulting in the isolation and structure elucidation of a new polyacetylene namely 5-octa-2,4,6-triynyl-furan-2(5H)-one. The structure of the isolated compound was determined based on IR, NMR (1D and 2D) and MS spectrometric data. The antitumor potential, including cytotoxicity to tumor cells and genotoxicity, was investigated. The results suggest that apoptotic cell death may have occurred, at least in part, via a caspasedependent mechanism.

Journal of the Brazilian Chemical Society, 2014

Neste trabalho, análises quimiométricas de espectros de NMR (ressonância magnética nuclear) de 31... more Neste trabalho, análises quimiométricas de espectros de NMR (ressonância magnética nuclear) de 31 P{ 1 H} obtidos de soro sanguíneo permitiram discriminar pacientes que sofreram acidente vascular encefálico isquêmico daqueles saudáveis devido a alterações na composição química de compostos contendo fósforo. Estes resultados indicam que análises metabonômicas por NMR de 31 P permitem conhecer melhor os mecanismos desencadeados pelo acidente vascular encefálico isquêmico. In this work, chemometric analyses over 31 P{ 1 H} NMR (nuclear magnetic ressonance) spectra of human blood serum permitted to discriminated ischemic stroke patients from health individuals due to changes in the chemical composition of phosphorus-containing compounds. These results indicate that 31 P NMR-based metabonomic allowed insights over the mechanism triggered by ischemic stroke.

Biochemical Systematics and Ecology, 2009

Journal of Analytical & Bioanalytical Techniques, 2013

It is known that cancer promotes changes in the metabolites found in biofluids and, therefore, th... more It is known that cancer promotes changes in the metabolites found in biofluids and, therefore, the early detection of these metabolites allows for the best prognosis of the disease. Since ESI-MS fingerprinting has proved to be an excellent tool for ‘‘first pass’’ metabolome analysis of complex biological samples, we have presented, for the first time, the use of direct infusion electrospray ionization low-resolution mass spectrometry associated with chemometric analysis (ESI-DIMS-PCA) for the detection of early stage cancer in mice. Ehrlich tumors were induced in Balb-C and Swiss mice, and blood was collected 1, 2, 3, 5, 7 and 10 days after tumor induction. Data from the mass spectra were analyzed via principal component analysis (PCA). Sample classifications were obtained within 3 days of tumor induction, which reflects a precocious time for cancer detection. ESI-DIMS-PCA was also used to detect cancer in groups of Swiss mice inoculated with sarcoma 180. Sample classifications were obtained 3 days after tumor induction, which indicates ESI-DIMS-PCA can be used to detect distinct neoplasms. Loading plot analysis demonstrated that the ions differentiating between the experimental and control groups are lysophosphatidylcholines and phosphatidylcholines. Partial least-squares analysis demonstrated that ESI-DIMS-PCA is capable of determining unknown samples in different stages of cancer development. ESI-DIMS with chemometric analysis is a promising technique for diagnosing cancer in the early stages without the need for invasive procedures.

Latin American Journal of Pharmacy, 2010

A simple and cost-effective method by capillary zone electrophoresis (CZE) was developed and vali... more A simple and cost-effective method by capillary zone electrophoresis (CZE) was developed and validated for the simultaneous determination of the antituberculosis drugs isoniazid (INH), pyrazinamide (PYR), and rifampicin (RIF) in tablets. A 40 mmol L -1 sodium tetraborate background electrolyte (BGE) solution (pH 9.0) was found to be suitable for separation of all the analytes. An uncoated fused-silica capillary of 64.5 cm length (effective length 56 cm) was used for chromatography separation. All analytes were completely separated within 5 min at an applied voltage of 20 kV (max. 50 μA), and detection was performed at 269.5 nm with an UV detector. The method was validated in terms of linearity, accuracy, precision, and robustness. The linearities of the calibration curves for INH, PYR, and RIF were 40-120 μg/mL (r 2 = 0.9994), 20-100 μg/mL (r 2 = 0.9997), and 40-100 μg/mL (r 2 = 0.9999), respectively. The proposed method was successfully applied for the simultaneous determination of the tuberculostatic drugs RIF, INH, and PYR in tablets. Thus, the proposed CZE method is a potential alternative to the HPLC methods described by the US Pharmacopoeia for the quality control of tuberculostatic drugs.

Natural product research, Jan 19, 2015

The essential oil obtained by hydrodistillation from leaves of Anaxagorea brevipes was analysed b... more The essential oil obtained by hydrodistillation from leaves of Anaxagorea brevipes was analysed by gas chromatography fitted with a flame ionisation detector (GC-FID) and coupled to mass spectrometry (GC-MS). Thirty one components were identified, representing around 75.7% of total oil. The major components were β-eudesmol (13.16%), α-eudesmol (13.05%), γ-eudesmol (7.54%), guaiol (5.12%), caryophyllene oxide (4.18%) and β-bisabolene (4.10%). The essential oil showed antimicrobial activity against Gram-positive bacteria and yeast with the MIC values between 25.0 and 100 μg/mL. The highest antiproliferative activity was observed for the oil against MCF-7 (breast, TGI = 12.8 μg/mL), NCI-H460 (lung, TGI = 13.0 μg/mL) and PC-3 (prostate, TGI = 9.6 μg/mL) cell lines, while against no cancer cell line HaCat (keratinocyte) the TGI was 38.8 μg/mL. The oil exhibited a small antioxidant activity assessed through ORAC-FL assay (517 μmol TE/g). This is the first report regarding the chemical com...

Revista Brasileira de Farmacognosia, 2008

... Ana Paula D. Pedroso I ; Sheila C. Santos I ; Ana A. Steil I ... Seeking to standardize guaco... more ... Ana Paula D. Pedroso I ; Sheila C. Santos I ; Ana A. Steil I ... Seeking to standardize guaco extract, which is officially registered in the Brazilian Pharmacopoea I (Brandão et al., 2006), this paper deals with the determination of kaurenoic acid through LC-PDA and the isolation ...