Francisco Kuhar - Academia.edu (original) (raw)

Papers by Francisco Kuhar

Lilloa, Aug 21, 2023

Hannibal ad portas: prediciendo la distribución potencial del hongo exótico degradador de madera ... more Hannibal ad portas: prediciendo la distribución potencial del hongo exótico degradador de madera Coniophora olivacea, nuevo en Patagonia Hannibal ad portas: predicting the potential distribution of the exotic wood decay fungus Coniophora olivacea, new in Patagonia ABSTRACT Coniophora olivacea has been traditionally considered as an important decay agent of building timbers of broadleaf and conifer woods, and causes significant damage on doors, window frames and other wood structures exposed to the environment. A more restricted role as tree pathogen has been also reported in cold climates. Studies of the predicted distribution of potentially invasive species are a useful tool to focus management efforts. Maximum-Entropy models allow predictions based on climatic and altitudinal layers as well as on previous data on the occurrence of species. The aim of this work is to report the presence of this wood decay fungus for the first time in Patagonia, and also to predict the potential spread pattern of this species using Maximum-Entropy.

International Journal of Medicinal Mushrooms, 2018

Please review the attached PDF file which contains the author proof of your article. This is your... more Please review the attached PDF file which contains the author proof of your article. This is your only opportunity to review the editing, typesetting, figure placement, and correctness of text, tables, and figures. Answer copyeditor's queries in the margin. Failure to answer queries will result in the delay of publication of your article, so please make sure they are all adequately addressed. You will not be charged for any corrections to editorial or typesetting errors; however, you will be billed at the rate of $25 per hour of production time for rewriting, rewording, or otherwise revising the article from the version accepted for publication ("author's alterations"); any such charges will be invoiced and must be paid before the article is published. Please return your corrections clearly marked on the page proofs or indicate your corrections in a list, specifying the location of the respective revisions as precisely as possible. Corrections must be uploaded onto the submission site, corrections are not accepted via email. If you wish to order offprints (see form below) please fill out and upload the form onto the submission site with your corrections. After uploading all files please be sure to click on the SUBMIT button so that we will be able to access your revisions. Please read the instructions carefully and if possible upload your corrections onto the submission site (http://submission.begellhouse.com/usr/login.html?prod_code=journals) within 48 hours, not including weekends. If you need more time, please let me know at your earliest convenience. No article will be published without confirmation of the author's review. If we do not hear from you within the allotted time, we will be happy to hold your article for a future issue, to give you more time to make your corrections.

Darwiniana, 2022

Lysurus fossatii, a new Lysuraceae species with unique macroscopic features is described and illu... more Lysurus fossatii, a new Lysuraceae species with unique macroscopic features is described and illustrated. Specimens were collected during field expeditions to remnants of the Espinal forest preserved at Estancia Yucat, South-East Córdoba, in central Argentina. The species is diagnosed by a light yellow peridium when immature, with the surface marked by a reticulum corresponding to the peridial sutures. When young and while still enclosed in the peridium, this species can be distinguished by the presence of a small white stipe completely covered by a green gleba. No actual receptacular structure is evidenced and only the stipe supports the gleba. Phylogenetic analyses highly supported the affinity of this species with L. sphaerocephalus in a close clade of L. cruciatus.

Mycologia, Jul 8, 2021

ABSTRACT In the Patagonian region, Cortinarius is the most diverse and abundant genus of ectomyco... more ABSTRACT In the Patagonian region, Cortinarius is the most diverse and abundant genus of ectomycorrhizal fungi with at least 250 species. Sequestrate forms were until recently documented within the genus Thaxterogaster, a genus now known to be polyphyletic, and many were consequently transferred to Cortinarius. Original descriptions were mostly available in German and Spanish and interpretations of morphological structures outdated. Despite recent advances in Cortinarius systematics, the current classification, diversity, and ecology of sequestrate “cortinarioid” fungi in Patagonia remain unclear. The objective of this study was to provide an update on sequestrate Cortinarius of southern South America. We documented each species with morphological descriptions, photographs, basidiospore scanning electron microscopy (SEM) images, and molecular characterization using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS) and nuc 28S rDNA (28S) sequence data. Original descriptions of taxa were also translated to English and revised based on fresh collections. We documented 24 species from Patagonia based on molecular data and conducted morphological and phylogenetic analysis for 18 previously described species based on type and reference specimens. In addition, we formally described two new species. Four additional taxa were provisionally determined as new but require further study. New ITS sequence data were produced from eight type specimens. We also provide a new name, Cortinarius gloiodes, nom. nov., for the taxon previously described as Thaxterogaster gliocyclus. In addition to the species treated in detail, we provided additional reference information and discussion on six described species that remained incompletely known or for which no recent collections were found. Of the 24 taxa documented from Patagonia, 15 species were assigned to 12 current sections in the genus Cortinarius. Analysis of spore ultrastructure showed that sequestrate forms of Patagonian Cortinarius lack a true perisporium.

Mycologia, Jan 2, 2019

Because of systematic sampling campaigns in the northern Patagonian Nothofagaceae forests of Arge... more Because of systematic sampling campaigns in the northern Patagonian Nothofagaceae forests of Argentina, several specimens of sequestrate fungi were collected. Some of those collections showed phylogenetic affinities and morphological similarities to members of the formerly recognized sequestrate genus Thaxterogaster, currently a synonym of Cortinarius on the basis of molecular data. Comparisons of macro-and micromorphological features and sequences of nuc rDNA internal transcribed spacer (ITS) regions have revealed that these collections belong to formerly undescribed species. The sequences of the four new taxa presented here, Cortinarius flavopurpureus, C. translucidus, C. nahuelhuapensis, and C. infrequens, were combined into a data set including additional sequences generated from herbarium collections and retrieved from public gene databases and analyzed by maximum likelihood and Bayesian inference methods. The four new species were resolved as distinct clades with strong support; at the same time, they showed unique morphological characteristics (hypogeous to subhypogeous habit, complete gasteromycetation, and spore shape and ornamentation) that separate them from previously described Cortinarius species. In addition, several undescribed and/or not previously sequenced species from these forests were detected through phylogenetic analysis of ectomycorrhizal root tip sequences. A key of characters to identify the sequestrate Cortinarius from Patagonia is provided.

Mycologia, Jul 1, 2016

The genusTomentellaforms abundant ectomycorrhizae in coniferous and deciduous forests worldwide. ... more The genusTomentellaforms abundant ectomycorrhizae in coniferous and deciduous forests worldwide. Molecular identification of root tips suggests undescribed species in theNothofagusforests of Patagonia, Argentina.Tomentella tenuissima,T. pulvinulataandT. patagonicaare described here as new to science based on morphological and molecular analyses. Their host range is addressed using available soil sequences. The identity of previous records ofT. galziniiandT. radiosaare discussed with morphological and molecular evidence.

Fungal Biology, Oct 1, 2017

The genus Descolea is emended in light of new and historical Patagonian collections. A new seques... more The genus Descolea is emended in light of new and historical Patagonian collections. A new sequestrate Descolea species is described from Patagonia. Two sequestrate species of Cortinarius (Thaxterogaster) are transferred to the genus Descolea. Morphological variation within Descolea and key characters for delimiting this genus are discussed.

Theory in Biosciences, Feb 1, 2022

Fungi show a high degree of morphological convergence. Regarded for a long time as an obstacle fo... more Fungi show a high degree of morphological convergence. Regarded for a long time as an obstacle for phylogenetic studies, homoplasy has also been proposed as a source of information about underlying morphogenetic patterning mechanisms. The "local-activation and long-range inhibition principle" (LALIP), underlying the famous reaction-diffusion model proposed by Alan Turing in 1952, appears to be one of the universal phenomena that can explain the ontogenetic origin of seriate patterns in living organisms. Reproductive structures of fungi in the class Agaricomycetes show a highly periodic structure resulting in, for example, poroid, odontoid, lamellate or labyrinthic hymenophores. In this paper, we claim that self-organized patterns might underlie the basic ontogenetic processes of these structures. Simulations based on LALIP-driven models and covering a wide range of parameters show an absolute mutual correspondence with the morphospace explored by extant agaricomycetes. This could not only explain geometric particularities but could also account for the limited possibilities displayed by hymenial configurations, thus making homoplasy a direct consequence of the limited morphospace resulting from the proposed patterning dynamics.

Persoonia, Jun 29, 2020

Molecular phylogenetic analyses have addressed the systematic position of several major Northern ... more Molecular phylogenetic analyses have addressed the systematic position of several major Northern Hemisphere lineages of Pezizales but the taxa of the Southern Hemisphere remain understudied. This study focuses on the molecular systematics and taxonomy of Southern Hemisphere species currently treated in the genera Under woodia and Gymnohydnotrya. Species in these genera have been identified as the monophyletic /gymnohydno trya lineage, but no further research has been conducted to determine the evolutionary origin of this lineage or its relationship with other Pezizales lineages. Here, we present a phylogenetic study of fungal species previously described in Underwoodia and Gymnohydnotrya, with sampling of all but one described species. We revise the taxonomy of this lineage and describe three new species from the Patagonian region of South America. Our results show that none of these Southern Hemisphere species are closely related to Underwoodia columnaris, the type species of the genus Underwoodia. Accordingly, we recognize the genus Geomorium described by Spegazzini in 1922 for G. fuegianum. We propose the new family, Geomoriaceae fam. nov., to accommodate this phylogenetically and morphologically unique Southern Hemisphere lineage. Molecular dating estimated that Geomoriaceae started to diverge from its sister clade Tuberaceae c. 112 MYA, with a crown age for the family in the late Cretaceous (c. 67 MYA). This scenario fits well with a Gondwanan origin of the family before the split of Australia and South America from Antarctica during the Paleocene-Eocene boundary (c. 50 MYA).

Lilloa, Oct 20, 2022

Tomentellopsis rosannae sp. nov. (Basidiomycota, Thelephorales), primera especie del género descr... more Tomentellopsis rosannae sp. nov. (Basidiomycota, Thelephorales), primera especie del género descripta del hemisferio sur Tomentellopsis rosannae sp. nov. (Basidiomycota, Thelephorales), first species in the genus described from the Southern Hemisphere

Nova Hedwigia, Aug 14, 2020

Collections of the genus Bovista in southern South America have been traditionally identified und... more Collections of the genus Bovista in southern South America have been traditionally identified under northern-hemisphere names. Although many puffball species show a wide distribution, the identity and distribution of Patagonian species still need to be revisited. Recent materials collected in Patagonia and displaying a unique capillitium ornamented with oblique membranes are shown here to correspond to a new species, Bovista pezica, from the Nothofagaceae forests. An unusually thick and coriaceous endoperidium also supports the separation of these specimens verified by our molecular analyses. We present here a full description of B. pezica sp. nov. along with a key for the species recorded in Patagonia, and we discuss the possible distributional range.

Fungal biology, 2019

This chapter compiles data on various aspects of ectomycorrhizal fungal taxa research, carried ou... more This chapter compiles data on various aspects of ectomycorrhizal fungal taxa research, carried out in South America (SA) since the first studies in the early 1900. Early research was achieved by foreign mycologists and the development of the first biological research centers in the region. The diversity of fungal taxa and data on the associated plant hosts and ecosystems are described based on biological and phytogeographic characteristics. Most publications on the topic were revised, the ectomycorrhizal lineages registered and species richness estimated based on fruiting bodies records and sequence data, derived from root tips of ectomycorrhizal hosts as well as OTU sequences from metabarcoding analysis. The review compiles previous and recent data for various areas of interest along the Andes, Guiana, Amazonian basin and the northeastern coast of SA. Additional research aspects such as physiology, cellular biology, chemistry, chemotaxonomy and mycosociology are briefly addressed.

Journal of Environmental Management, Jul 1, 2013

Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials ... more Bioremediation of contaminated sites by biosorption of pollutants onto a wide range of materials has emerged as a promising treatment for recalcitrant aromatic compounds or heavy metals. When adsorption occurs on living white-rot fungi mycelia, the pollutants may be degraded by ligninolytic enzymes. However, the survival of mycelia in harsh conditions is one of the drawbacks of those methodologies. In this study, it was demonstrated that culture media supplemented with several guaiacol derivatives (vanilloids) increased the resistance of Ganoderma lucidum E47 cultures to chemical stress by enhancing the adsorptive capacity of the extracellular mucilaginous material (ECMM). The toxicity of the fungicides gentian violet (GV), malachite green (MG) and clotrimazole, and the heavy metal Cadmium was noticeably diminished in fungal cultures supplemented with the guaiacol derivative vanillic acid (VA). No degradation of the tested compounds was detected. The activity of the oxidative enzymatic systems like laccase, a well-known oxidase associated to dye degradation, was only detectable after complete growth on plates. Extremely low concentrations of VA caused a significant protective effect, radial extension of the growth halo in plates supplemented with 0.0001 mM of VA plus GV was up to 20% to that obtained in control plates (without addition of GV and VA). Therefore, the protective effect could not be attributable to VA per se. ECMM separated from the mycelium exhibited a much higher increase in the adsorptive capacity when isolated from liquid cultures containing VA, while that obtained from unsupplemented cultures showed an almost null adsorptive capacity.

Boletín micológico, Jul 10, 2017

Dos registros de Agaricales (Basidiomycota) exóticos en bosques nativos.-Romano G.. et al

International Journal of Medicinal Mushrooms, 2022

Mycologia, Jul 1, 2009

A new species, Geastrum episcopale, is described from Argentina. Detailed descriptions and illust... more A new species, Geastrum episcopale, is described from Argentina. Detailed descriptions and illustrations of fresh and dry basidiomata are provided along with photographs of microscopic elements, such as spores, capillitium, calcium oxalate crystals, and exo-and endoperidium surfaces. This fungus is typical of the genus in its morphology and ecology but clearly differs from every known species. This species can be distinguished easily by the red-violet (to magenta) exoperidium and the lilac endoperidium. Specimens were collected on wet soil composed of a superficial layer of ca. 5 cm leaf litter and 15 cm of particulate and melanized lignocellulosic material (pH 6.5) under Eugenia uruguayensis and Nectandra sp.

Maderas. Ciencia y tecnología

Mycelium composites consist of particulate lignocellulosic materials, e.g., sawdust from the timb... more Mycelium composites consist of particulate lignocellulosic materials, e.g., sawdust from the timber industry structured as a solid matrix resulting from the mycelial growth. Many protocols have been proposed based on different strains and substrates. However, the influence of intrinsic elements, such as the structure of the hyphal system on the main parameters required by the industry still needs to be researched. The main goal of this work is to assess the performance of five Patagonian lignocellulolytic fungal species for producing mycelium composites. Strains of these species were studied in order to assess the relation between basidiome hyphal structure and the hyphal structure of mycelium-based materials. Comparisons of the hardness in the Janka scale were performed with commercial expanded polystyrene. Composites resulting from the growth of Pleurotus ostreatus, Nothophellinus andinopatagonicus and Funalia trogii successfully formed composites, showing a lower quality than Ganoderma austral. Ryvardenia cretacea in turn completely failed to colonize the substrate. The material resulting from the growth of Ganoderma australe on pine sawdust (a substrate chosen based on its local availability) is proposed as a good substitute with improved resistance.

Boletín micológico, Nov 21, 2016

Identificación del oídio causado por Erysiphe trifoliorum (Wallr.) U. Braun en Melilotus officina... more Identificación del oídio causado por Erysiphe trifoliorum (Wallr.) U. Braun en Melilotus officinalis. Identification of the powdery mildew caused by Erysiphe trifoliorum (Wallr.