Gabriela Gallardo - Academia.edu (original) (raw)

Papers by Gabriela Gallardo

Food Research International, 2006

The encapsulation of fish oil in electrosprayed capsules using whey protein and carbohydrates (pu... more The encapsulation of fish oil in electrosprayed capsules using whey protein and carbohydrates (pullulan and dextran or glucose syrup) mixtures as glassy wall materials was studied. Capsules with fish oil emulsified by using only a rotor-stator emulsification exhibited higher oxidative stability than capsules where the oil was emulsified by high-pressure homogenization. Moreover, glucose syrup capsules (with a peroxide value, PV, of 19.7 ± 4.4 meq/kg oil and a content of 1-penten-3-ol of 751.0 ± 69.8 ng/g oil) were less oxidized than dextran capsules after 21 days of storage at 20 C (PV of 24.9 ± 0.4 meq/kg oil and 1penten-3-ol of 1161.0 ± 222.0 ng/g oil). This finding may be attributed to differences in oxygen permeability between both types of capsules. These results indicated the potential of both combinations of whey protein, pullulan, and dextran or glucose syrup as shell materials for the encapsulation of omega-3 PUFA in nano-microcapsules obtained by electrospraying.

Foods

Sacha inchi (Plukenetia huayllabambana) oil was co-microencapsulated with natural antioxidant ext... more Sacha inchi (Plukenetia huayllabambana) oil was co-microencapsulated with natural antioxidant extracts (NAE), such as camu-camu (Myrciaria dubia (HBK) Mc Vaugh) fruit, Añil variety Andean potato (Solanum tuberosum andigenum, and elderberry fruit (Sambucus peruviana). Gum Arabic and the ternary combination of gum Arabic (GA) + maltodextrin (MD) + whey protein isolate (WPI) at different formulations were used as coating materials for the encapsulation process using spray-drying. The moisture content, particle size distribution and morphology, total phenolic content, antioxidant activity, fatty acid and sterol composition, oxidative stability, and shelf-life were evaluated. Co-microcapsules of sacha inchi (P. huayllabambana) oil with camu camu skin extract (CCSE) at 200 ppm encapsulated with GA + MD + WPI had the highest total polyphenol content (4239.80 µg GAE/g powder), antioxidant activity (12,454.00 µg trolox/g powder), omega-3 content (56.03%), β-sitosterol (62.5%), greater oxidat...

Antioxidants

Sacha inchi Plukenetia huayllabambana oil (SIPHO) was co-microencapsulated, by spray drying using... more Sacha inchi Plukenetia huayllabambana oil (SIPHO) was co-microencapsulated, by spray drying using gum arabic as a coating material, with antioxidant extracts of camu camu (Myrciaria dubia (HBK) McVaugh) (CCSE) and mango (Mangifera indica) (MSE) skins obtained by ultrasound–microwave-assisted extraction (UMAE). The physicochemical characteristics of the microcapsules, such as, particle size, morphology, and moisture, as well as the encapsulation efficiency, the fatty acid composition, and oxidative stability, were determined in order to select the best formulation for the design of functional powdered beverages. The formulation with the highest amounts of ω3 acids and polyphenols was used to prepare a functional powdered beverage that contained ω3 (52.74%), antioxidant activity (324.80 mg AAE/100 g powder), and acceptable sensory attributes.

La Alimentación Latinoamericana, No. 316, 2015, 2015

World Journal of Microbiology and Biotechnology, 2004

A search for bioactive compounds, inhibitors of Paenibacillus larvae, the causal agent of America... more A search for bioactive compounds, inhibitors of Paenibacillus larvae, the causal agent of American foulbrood, a honeybees' disease, was carried on. Extracts of two fungal strains, Alternaria brassicicola and Alternaria raphani, isolated from pollen collected from beehives, exhibited a specific inhibitory activity against this bacterium. From these extracts and by means of chromatographic steps and bioassay-guided fractionation, three tetramic acids were isolated. The compounds were identified by spectroscopic methods and the absolute stereochemistry was chemically determined. L-Tenuazonic acid was shown to be responsible for the antibiotic activity. This compound showed a MIC of 32 lg/ml, comparable with that of oxytetracycline, an antibiotic currently used for the prevention of American foulbrood. Fungal strains Alternaria brassicicola (Schw.) Wiltshire and A. raphani Groves & Skolko were isolated from pollen and classified, according to the taxonomic criteria by Ellis

Phytochemistry Letters, 2008

Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the... more Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the honey bee entomopathogenic fungus Ascosphaera apis and their structures were determined by spectroscopic methods. These compounds resembled certain pheromones of honey bees. Compounds 1 and 2 showed weak antifungal and antioxidant properties and 1 exhibited antibiotic activity. The compound p-hydroxyphenylethanol, showing antifungal properties, was also isolated and identified.

En esta tesis se describe el aislamiento, bioguiado a traves de ensayos de actividad antifungica ... more En esta tesis se describe el aislamiento, bioguiado a traves de ensayos de actividad antifungica y/o antibacteriana, y la elucidacion estructural de los metabolitos presentes en los cultivos de hongos provenientes de diversas fuentes naturales. Las especies analizadas Alternaria brassicicola, A. raphani, Aspergillus restrictus, Trichoderma koniigii y Ascosphaera apis fueron aisladas de polen de colmenas ubicadas en el apiario del INTA (Instituto Nacional de Tecnologia Agropecuaria) de la ciudad de Balcarce, Buenos Aires, Argentina. De Alternaria brassicicola y A. raphani se aislaron los compuestos 1-4, siendo el acido tenuazonico, compuesto 2, el responsable de la actividad antibacteriana frente a la bacteria P. larvae, patogena de las larvas de las abejas meliferas. El compuesto 1, acido D-allo-tenuazonico, es aislado por primera vez como producto natural. De Aspergillus restrictus se aislo el compuesto 3, conocido como pseurotina A3. Del hongo entomopatogeno de las larvas de las a...

Proceedings

Sacha inchi (Plukenetia huayllabambana) oil was microencapsulated by spray drying with gum arabic... more Sacha inchi (Plukenetia huayllabambana) oil was microencapsulated by spray drying with gum arabic and with extracts of camu camu (Myrciaria dubia (HBK) Mc Vaugh) and mango (Mangifera indica) skins, obtained by assisted microwave. The physicochemical characteristics, such as moisture content, encapsulation efficiency, particle size, morphology, fatty acid composition and oxidative stability, were evaluated in order to select the best formulation for the development of functional foods. The most important results indicate that the microcapsules formulated with extracts of the fruit skins provide greater protection to sacha inchi oil (P. huayllabambana) against oxidation compared to commercial antioxidant BHT (Butylated Hydroxytoluene), resulting in a slight loss of ω-3 fatty acids.

Foods

Sacha inchi (Plukenetia huayllabambana L. and Plukenetia volubilis L.) edible oils were microenca... more Sacha inchi (Plukenetia huayllabambana L. and Plukenetia volubilis L.) edible oils were microencapsulated and the lipid fraction of the microparticles was characterized. Hi-cap®, Capsule®, Arabic gum, and the binary combination of Arabic gum + maltodextrin and the ternary combination of Arabic gum + maltodextrin + whey protein isolate, were used as coating materials for the encapsulation process using spray-drying. The surface and the total oils obtained from the microparticles were evaluated in terms of fatty acid composition, minor glyceride polar compounds, polymers, oxidized triglycerides, diglycerides, monoglycerides, and free fatty acids, along with their unsaponifiable components, sterols, and tocopherols. Differences between the original oils and the microencapsulated ones were determined. The most remarkable results included the presence of polymers when there were none in the original oils, the slight loss in ω3-fatty acids, up to 6%, the loss in tocopherols, in some of th...

Chemistry & biodiversity, Jan 30, 2018

Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp.... more Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp., isolated from the roots of rye grass (Lollium sp.) and cultured under different experimental conditions, are described here for the first time. The use of suberoylanilide hydroxamic acid (SAHA) and other histone deacetylase inhibitors as epigenetic modifiers in the culture medium was evaluated by LC/MS and LC/MS/MS. Several differences in the metabolite production were detected by means of supervised principal component analysis (PCA) of LC/MS data. The presence of the compounds in the culture medium or in the mycelium was compared. The structure of many of the metabolites was confirmed by isolation from a larger scale culture. These metabolites were characterized as prenylhydroxybenzoic acids and chromans, two compounds, one of each class were previously undescribed, prenylquinoids, diketopiperazines and macrosphelides. Some of the compounds which were released to the medium showed go...

Microencapsulation and Microspheres for Food Applications, 2015

Phytochemistry Letters, 2008

Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the... more Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the honey bee entomopathogenic fungus Ascosphaera apis and their structures were determined by spectroscopic methods. These compounds resembled certain pheromones of honey bees. Compounds 1 and 2 showed weak antifungal and antioxidant properties and 1 exhibited antibiotic activity. The compound p-hydroxyphenylethanol, showing antifungal properties, was also isolated and identified. ß

Phytochemistry, 2006

In a screening for antimicrobial metabolites, amides of D-allo-and L-isoleucine derivatives were ... more In a screening for antimicrobial metabolites, amides of D-allo-and L-isoleucine derivatives were isolated from the culture of a marine strain of Acremonium furcatum. Structural elucidation of these compounds was performed by analysis of spectroscopic data and confirmed by synthesis. All of the compounds, natural and synthetic intermediates, were bioassayed against bacteria and phytopathogenic fungi, with many showing remarkable antifungal activities.

Food Research International, 2013

ABSTRACT Health benefits associated to ω-3 fatty acids consumption together with the high suscept... more ABSTRACT Health benefits associated to ω-3 fatty acids consumption together with the high susceptibility to oxidation of ω-3 containing oils have led to the development of microencapsulated oils for nutraceutical and food enrichment applications. The aim of this work is to obtain different formulations for linseed oil microencapsulation by spray-drying with high encapsulation efficiency and evaluate their resistance to oxidation through the accelerated Rancimat test. Four formulations were tested; using different combinations of gum arabic (GA), maltodextrin (MD), methyl cellulose (MC) and whey protein isolate (WPI). Microcapsules made of 100% GA and ternary mixtures of GA, MD and WPI presented the highest protection from oxidation and microencapsulation efficiencies higher than 90%. They also presented spherical structures with smooth surfaces which kept unaltered after 10-month storage. GA containing formulation was included in bread manufacturing. Fortified bread resulted similar in appearance to control bread without microcapsules, but α-linolenic acid content was reduced significantly after preparation.

Food Research International, 2006

The encapsulation of fish oil in electrosprayed capsules using whey protein and carbohydrates (pu... more The encapsulation of fish oil in electrosprayed capsules using whey protein and carbohydrates (pullulan and dextran or glucose syrup) mixtures as glassy wall materials was studied. Capsules with fish oil emulsified by using only a rotor-stator emulsification exhibited higher oxidative stability than capsules where the oil was emulsified by high-pressure homogenization. Moreover, glucose syrup capsules (with a peroxide value, PV, of 19.7 ± 4.4 meq/kg oil and a content of 1-penten-3-ol of 751.0 ± 69.8 ng/g oil) were less oxidized than dextran capsules after 21 days of storage at 20 C (PV of 24.9 ± 0.4 meq/kg oil and 1penten-3-ol of 1161.0 ± 222.0 ng/g oil). This finding may be attributed to differences in oxygen permeability between both types of capsules. These results indicated the potential of both combinations of whey protein, pullulan, and dextran or glucose syrup as shell materials for the encapsulation of omega-3 PUFA in nano-microcapsules obtained by electrospraying.

Foods

Sacha inchi (Plukenetia huayllabambana) oil was co-microencapsulated with natural antioxidant ext... more Sacha inchi (Plukenetia huayllabambana) oil was co-microencapsulated with natural antioxidant extracts (NAE), such as camu-camu (Myrciaria dubia (HBK) Mc Vaugh) fruit, Añil variety Andean potato (Solanum tuberosum andigenum, and elderberry fruit (Sambucus peruviana). Gum Arabic and the ternary combination of gum Arabic (GA) + maltodextrin (MD) + whey protein isolate (WPI) at different formulations were used as coating materials for the encapsulation process using spray-drying. The moisture content, particle size distribution and morphology, total phenolic content, antioxidant activity, fatty acid and sterol composition, oxidative stability, and shelf-life were evaluated. Co-microcapsules of sacha inchi (P. huayllabambana) oil with camu camu skin extract (CCSE) at 200 ppm encapsulated with GA + MD + WPI had the highest total polyphenol content (4239.80 µg GAE/g powder), antioxidant activity (12,454.00 µg trolox/g powder), omega-3 content (56.03%), β-sitosterol (62.5%), greater oxidat...

Antioxidants

Sacha inchi Plukenetia huayllabambana oil (SIPHO) was co-microencapsulated, by spray drying using... more Sacha inchi Plukenetia huayllabambana oil (SIPHO) was co-microencapsulated, by spray drying using gum arabic as a coating material, with antioxidant extracts of camu camu (Myrciaria dubia (HBK) McVaugh) (CCSE) and mango (Mangifera indica) (MSE) skins obtained by ultrasound–microwave-assisted extraction (UMAE). The physicochemical characteristics of the microcapsules, such as, particle size, morphology, and moisture, as well as the encapsulation efficiency, the fatty acid composition, and oxidative stability, were determined in order to select the best formulation for the design of functional powdered beverages. The formulation with the highest amounts of ω3 acids and polyphenols was used to prepare a functional powdered beverage that contained ω3 (52.74%), antioxidant activity (324.80 mg AAE/100 g powder), and acceptable sensory attributes.

La Alimentación Latinoamericana, No. 316, 2015, 2015

World Journal of Microbiology and Biotechnology, 2004

A search for bioactive compounds, inhibitors of Paenibacillus larvae, the causal agent of America... more A search for bioactive compounds, inhibitors of Paenibacillus larvae, the causal agent of American foulbrood, a honeybees' disease, was carried on. Extracts of two fungal strains, Alternaria brassicicola and Alternaria raphani, isolated from pollen collected from beehives, exhibited a specific inhibitory activity against this bacterium. From these extracts and by means of chromatographic steps and bioassay-guided fractionation, three tetramic acids were isolated. The compounds were identified by spectroscopic methods and the absolute stereochemistry was chemically determined. L-Tenuazonic acid was shown to be responsible for the antibiotic activity. This compound showed a MIC of 32 lg/ml, comparable with that of oxytetracycline, an antibiotic currently used for the prevention of American foulbrood. Fungal strains Alternaria brassicicola (Schw.) Wiltshire and A. raphani Groves & Skolko were isolated from pollen and classified, according to the taxonomic criteria by Ellis

Phytochemistry Letters, 2008

Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the... more Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the honey bee entomopathogenic fungus Ascosphaera apis and their structures were determined by spectroscopic methods. These compounds resembled certain pheromones of honey bees. Compounds 1 and 2 showed weak antifungal and antioxidant properties and 1 exhibited antibiotic activity. The compound p-hydroxyphenylethanol, showing antifungal properties, was also isolated and identified.

En esta tesis se describe el aislamiento, bioguiado a traves de ensayos de actividad antifungica ... more En esta tesis se describe el aislamiento, bioguiado a traves de ensayos de actividad antifungica y/o antibacteriana, y la elucidacion estructural de los metabolitos presentes en los cultivos de hongos provenientes de diversas fuentes naturales. Las especies analizadas Alternaria brassicicola, A. raphani, Aspergillus restrictus, Trichoderma koniigii y Ascosphaera apis fueron aisladas de polen de colmenas ubicadas en el apiario del INTA (Instituto Nacional de Tecnologia Agropecuaria) de la ciudad de Balcarce, Buenos Aires, Argentina. De Alternaria brassicicola y A. raphani se aislaron los compuestos 1-4, siendo el acido tenuazonico, compuesto 2, el responsable de la actividad antibacteriana frente a la bacteria P. larvae, patogena de las larvas de las abejas meliferas. El compuesto 1, acido D-allo-tenuazonico, es aislado por primera vez como producto natural. De Aspergillus restrictus se aislo el compuesto 3, conocido como pseurotina A3. Del hongo entomopatogeno de las larvas de las a...

Proceedings

Sacha inchi (Plukenetia huayllabambana) oil was microencapsulated by spray drying with gum arabic... more Sacha inchi (Plukenetia huayllabambana) oil was microencapsulated by spray drying with gum arabic and with extracts of camu camu (Myrciaria dubia (HBK) Mc Vaugh) and mango (Mangifera indica) skins, obtained by assisted microwave. The physicochemical characteristics, such as moisture content, encapsulation efficiency, particle size, morphology, fatty acid composition and oxidative stability, were evaluated in order to select the best formulation for the development of functional foods. The most important results indicate that the microcapsules formulated with extracts of the fruit skins provide greater protection to sacha inchi oil (P. huayllabambana) against oxidation compared to commercial antioxidant BHT (Butylated Hydroxytoluene), resulting in a slight loss of ω-3 fatty acids.

Foods

Sacha inchi (Plukenetia huayllabambana L. and Plukenetia volubilis L.) edible oils were microenca... more Sacha inchi (Plukenetia huayllabambana L. and Plukenetia volubilis L.) edible oils were microencapsulated and the lipid fraction of the microparticles was characterized. Hi-cap®, Capsule®, Arabic gum, and the binary combination of Arabic gum + maltodextrin and the ternary combination of Arabic gum + maltodextrin + whey protein isolate, were used as coating materials for the encapsulation process using spray-drying. The surface and the total oils obtained from the microparticles were evaluated in terms of fatty acid composition, minor glyceride polar compounds, polymers, oxidized triglycerides, diglycerides, monoglycerides, and free fatty acids, along with their unsaponifiable components, sterols, and tocopherols. Differences between the original oils and the microencapsulated ones were determined. The most remarkable results included the presence of polymers when there were none in the original oils, the slight loss in ω3-fatty acids, up to 6%, the loss in tocopherols, in some of th...

Chemistry & biodiversity, Jan 30, 2018

Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp.... more Secondary metabolites from the cultures of the dark septate fungal endophyte (DSE) Drechslera sp., isolated from the roots of rye grass (Lollium sp.) and cultured under different experimental conditions, are described here for the first time. The use of suberoylanilide hydroxamic acid (SAHA) and other histone deacetylase inhibitors as epigenetic modifiers in the culture medium was evaluated by LC/MS and LC/MS/MS. Several differences in the metabolite production were detected by means of supervised principal component analysis (PCA) of LC/MS data. The presence of the compounds in the culture medium or in the mycelium was compared. The structure of many of the metabolites was confirmed by isolation from a larger scale culture. These metabolites were characterized as prenylhydroxybenzoic acids and chromans, two compounds, one of each class were previously undescribed, prenylquinoids, diketopiperazines and macrosphelides. Some of the compounds which were released to the medium showed go...

Microencapsulation and Microspheres for Food Applications, 2015

Phytochemistry Letters, 2008

Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the... more Two yet unknown from natural sources monoterpenoids, 1 and 2, were isolated from a culture of the honey bee entomopathogenic fungus Ascosphaera apis and their structures were determined by spectroscopic methods. These compounds resembled certain pheromones of honey bees. Compounds 1 and 2 showed weak antifungal and antioxidant properties and 1 exhibited antibiotic activity. The compound p-hydroxyphenylethanol, showing antifungal properties, was also isolated and identified. ß

Phytochemistry, 2006

In a screening for antimicrobial metabolites, amides of D-allo-and L-isoleucine derivatives were ... more In a screening for antimicrobial metabolites, amides of D-allo-and L-isoleucine derivatives were isolated from the culture of a marine strain of Acremonium furcatum. Structural elucidation of these compounds was performed by analysis of spectroscopic data and confirmed by synthesis. All of the compounds, natural and synthetic intermediates, were bioassayed against bacteria and phytopathogenic fungi, with many showing remarkable antifungal activities.

Food Research International, 2013

ABSTRACT Health benefits associated to ω-3 fatty acids consumption together with the high suscept... more ABSTRACT Health benefits associated to ω-3 fatty acids consumption together with the high susceptibility to oxidation of ω-3 containing oils have led to the development of microencapsulated oils for nutraceutical and food enrichment applications. The aim of this work is to obtain different formulations for linseed oil microencapsulation by spray-drying with high encapsulation efficiency and evaluate their resistance to oxidation through the accelerated Rancimat test. Four formulations were tested; using different combinations of gum arabic (GA), maltodextrin (MD), methyl cellulose (MC) and whey protein isolate (WPI). Microcapsules made of 100% GA and ternary mixtures of GA, MD and WPI presented the highest protection from oxidation and microencapsulation efficiencies higher than 90%. They also presented spherical structures with smooth surfaces which kept unaltered after 10-month storage. GA containing formulation was included in bread manufacturing. Fortified bread resulted similar in appearance to control bread without microcapsules, but α-linolenic acid content was reduced significantly after preparation.