Grazia Marino - Academia.edu (original) (raw)
Papers by Grazia Marino
Plant Growth Regulation, 1988
The effect of paclobutrazol on in vitro rooting and growth of sour cherry (Prunus cerasus) rootst... more The effect of paclobutrazol on in vitro rooting and growth of sour cherry (Prunus cerasus) rootstock CAB 11E clone, of S 749 × S 1490 (Prunus persica × Prunus kansuensis) hybrid rootstock, and of pear (Pyrus communis), cv. Abbé Fetel is reported. PP333 increased rooting of S 749 × S 1490 and of Abbé Fetel, particularly at a concentration of 0.5 mg/l (a.i.); moreover, it induced a rooting percentage as high as auxin in the former and hastened rooting of the latter. By contrast, paclobutrazol did not affect root production of 11 E. PP333-treated plants had shorter and thicker roots than controls but similar survival rates during acclimatization. Otherwise they grew less than controls during the first part of the acclimatization phase.
The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, m... more The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, morphogenesis (in leaf explants), and oxidative stress reactions in in vitro cultures of ‘BA 29’ quince were investigated. Aluminium (Al 0.5mM) strongly inhibited shoot growth in the proliferation and rooting phases (Al 2.2mM), reduced shoot proliferation (Al 1.1mM), and induced tissue browning. Superoxide dismutase (SOD)
Scientia Horticulturae, 2010
Procedures for cold storage of in vitro cultures can delay subculturing, reducing production cost... more Procedures for cold storage of in vitro cultures can delay subculturing, reducing production costs and risks of contamination and somaclonal variation. The present work investigates the effects of media with sorbitol (116.8mM, medium SO) or sucrose (58.4mM) alone (medium SU), or the latter in combination with mannitol (58.4mM, medium M) on 7-month storage at 5°C of apricot shoots, cv San
![Research paper thumbnail of In vitro [14C]-labelled 6-benzyladenine uptake and14CO2 evolution in two Japanese plum cultivars](https://attachments.academia-assets.com/46504860/thumbnails/1.jpg)
](Plant Cell Tissue and Organ Culture, 1988
Japanese plum cultivars ‘Obilnaga’ and ‘Santa Rosa’ showed different proliferation responses when... more Japanese plum cultivars ‘Obilnaga’ and ‘Santa Rosa’ showed different proliferation responses when grown in similar conditions in vitro. This led us to investigate BA uptake by shoots of both cultivars grown for different times (15 hrs, 1, 3, 6, 9, 13, 16 and 20 days) on an incubation medium enriched with 10μCi (370 kBq) 8-[14C]BA/250 ml. The decrease of radioactivity in the medium and its increase in the EtOH-soluble and-insoluble fractions of shoots of both cultivars grown for different times (15 h, 1, 3, 6, 9, 13, 16 and 20 days) on cultivars. Increasing amounts of14CO2 were detected in the culture atmosphere when shoots were grown in gas-tight vials. The type of container closure strongly affected proliferation. From the results reported here it is not possible to state whether different optimal subculture intervals and different proliferation responses of ‘Santa Rosa’ and ‘Obilnaja’ cultures are due to different tissue sensitivity to the cytokinin and/or to different metabolic activities. Nevertheless the highest proliferation rates of both plums are clearly related to a drop of EtOH-soluble radioactivity of shoots by the end of subculture.
Summary Shoots of “San Castrese” and “Portici” apricots (Prunus armeniaca L.) free of cultivable... more Summary Shoots of “San Castrese” and “Portici” apricots (Prunus armeniaca L.) free of cultivable bacteria, shoots of the same origin exhibiting bacterial contamination after repeated subcultures, and contaminated shoots treated with cefotaxime were compared for gas exchange, proliferation rate, and fresh and dry weight. Cultures of San Castrese contaminated byBacillus circulans andSphingomonas paucimobilis, and of Portici contaminated withStaphylococcus hominis andMicrococcus kristinae,
Developments in Plant Pathology, 1997
... OF BACTERIAL CONTAMINANTS AND GAS EVOLUTION IN APRICOT SHOOT CULTURESGRAZIA MARINO1, NANNI ..... more ... OF BACTERIAL CONTAMINANTS AND GAS EVOLUTION IN APRICOT SHOOT CULTURESGRAZIA MARINO1, NANNI ... and cultured on proliferation media enriched with 28 and 50^ M ASA or ... 6. Magnanini, E. and Ventura, M.(1994) in'II Giornate Scientifiche SOI 1994', 22-24 ...
in Vitro Cellular & Developmental Biology-plant, 2004
The effect of different sealing materials [i.e., polyvinyl chloride (PVC) transparent film, and P... more The effect of different sealing materials [i.e., polyvinyl chloride (PVC) transparent film, and Parafilm (PARA) for Petri dishes was investigated on shoot regeneration from quince (Cydonia oblonga L.) ‘BA 29’ leaf explants. Leaves were excised from proliferating shoot cultures, transversally scored, and placed with the abaxial side down in 60-mm Petri dishes containing 10 ml of Murashige and Skoog modified medium, with 5.4 μM α-naphthaleneacetic acid, 4.5 μM thidiazuron, 200 mg l−1 cefotaxime, and 0.25% (w/v) Phytagel (IM medium) for shoot bud induction, and cultured in darkness at 22±2°C for 28 d. Then the explants were transferred to standard conditions (16-h photoperiod at 30 μmol m−2 s−1 photosynthetically active radiation) on a medium similar to IM, except for lack of NAA, and with 0.65% (w/v) agar instead of Phytagel, for an additional 15–28 d. The sealing combinations PARA-PARA, PARA-PVC, PVC-PARA, and PVC-PVC (in the induction-expression phases) were compared during regeneration and for their carry-over effect on shoot development after transfer of explants to an elongation medium (0.9 μM 6-benzyladenine). Carbon dioxide accumulated at 27.2 mmol mol−1 at the end of induction, and gradually decreased from 35.4 mmol mol−1 on day 9 to 22.5 mmol mol−1 on day 28 of the expression phase in PARA-sealed Petri dishes, being always much higher than after sealing with PVC (1–2 mmol mol−1). Ethylene concentration was 0.1 and 0.04 μmol mol−1 in the first part of the induction and expression phase, respectively, in PARA-sealed Petri dishes, and slightly decreased with duration of exposure to light during expression; while it was absent in most PVC-sealed dishes. The PARA-PARA and PVC-PVC (induction-expression) combinations gave, respectively, the worst and best results of regeneration and successive shoot development.
Plant Cell Tissue and Organ Culture, 1993
In vitro proliferation and rooting capacity of ‘San Castrese’ and ‘Portici’ apricots (Prunus arme... more In vitro proliferation and rooting capacity of ‘San Castrese’ and ‘Portici’ apricots (Prunus armeniaca L.) were tested on modified MS medium enriched with varying growth regulator concentrations and sucrose (58.4 mM) or sorbitol (116.8 mM) as main carbon energy sources. The interaction of proliferation and rooting media was also studied. Proliferation of both cultivars was proportional to benzyladenine (BA) concentration and enhanced with sorbitol media. However, 8.8 μM BA was often associated with hyperhydricity, particularly when shoots were grown on sucrose media. Newly proliferated shoots elongated better on sorbitol media. The positive influence of sorbitol on proliferation and shoot growth was not due to osmotic effects. Moreover, sorbitol showed a positive carryover effect in hastening rooting of ‘Portici’. By contrast, when transferred to sorbitol rooting media, the shoots of both cultivars generally showed low rooting, with short, thick roots. Up to 70% of the plantlets that produced roots in sucrose media enriched with indolebutyric acid were successfully acclimatized when they were dipped in a benomyl (0.075% w/v) suspension before being transplanted with care being taken to prevent over-wetting of soil.
Plant Cell, Tissue and Organ Culture, 1985
In Vitro Cellular & Developmental Biology-Plant, 1997
Summary Treatments differing from each other for the type of tube closure (ie, cotton plug for fr... more Summary Treatments differing from each other for the type of tube closure (ie, cotton plug for free gas exchange, airtight rubber cap, and rubber cap with ethysorb) and/or rooting culture medium (ie, enriched or not by 25 to 100 µM acetylsalicylic acid) were compared for their ...
… journal of plant …, 2009
Plant Growth Regulation, 1988
The effect of paclobutrazol on in vitro rooting and growth of sour cherry (Prunus cerasus) rootst... more The effect of paclobutrazol on in vitro rooting and growth of sour cherry (Prunus cerasus) rootstock CAB 11E clone, of S 749 × S 1490 (Prunus persica × Prunus kansuensis) hybrid rootstock, and of pear (Pyrus communis), cv. Abbé Fetel is reported. PP333 increased rooting of S 749 × S 1490 and of Abbé Fetel, particularly at a concentration of 0.5 mg/l (a.i.); moreover, it induced a rooting percentage as high as auxin in the former and hastened rooting of the latter. By contrast, paclobutrazol did not affect root production of 11 E. PP333-treated plants had shorter and thicker roots than controls but similar survival rates during acclimatization. Otherwise they grew less than controls during the first part of the acclimatization phase.
The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, m... more The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, morphogenesis (in leaf explants), and oxidative stress reactions in in vitro cultures of ‘BA 29’ quince were investigated. Aluminium (Al 0.5mM) strongly inhibited shoot growth in the proliferation and rooting phases (Al 2.2mM), reduced shoot proliferation (Al 1.1mM), and induced tissue browning. Superoxide dismutase (SOD)
Scientia Horticulturae, 2010
Procedures for cold storage of in vitro cultures can delay subculturing, reducing production cost... more Procedures for cold storage of in vitro cultures can delay subculturing, reducing production costs and risks of contamination and somaclonal variation. The present work investigates the effects of media with sorbitol (116.8mM, medium SO) or sucrose (58.4mM) alone (medium SU), or the latter in combination with mannitol (58.4mM, medium M) on 7-month storage at 5°C of apricot shoots, cv San
Plant Cell Tissue and Organ Culture, 1988
Japanese plum cultivars ‘Obilnaga’ and ‘Santa Rosa’ showed different proliferation responses when... more Japanese plum cultivars ‘Obilnaga’ and ‘Santa Rosa’ showed different proliferation responses when grown in similar conditions in vitro. This led us to investigate BA uptake by shoots of both cultivars grown for different times (15 hrs, 1, 3, 6, 9, 13, 16 and 20 days) on an incubation medium enriched with 10μCi (370 kBq) 8-[14C]BA/250 ml. The decrease of radioactivity in the medium and its increase in the EtOH-soluble and-insoluble fractions of shoots of both cultivars grown for different times (15 h, 1, 3, 6, 9, 13, 16 and 20 days) on cultivars. Increasing amounts of14CO2 were detected in the culture atmosphere when shoots were grown in gas-tight vials. The type of container closure strongly affected proliferation. From the results reported here it is not possible to state whether different optimal subculture intervals and different proliferation responses of ‘Santa Rosa’ and ‘Obilnaja’ cultures are due to different tissue sensitivity to the cytokinin and/or to different metabolic activities. Nevertheless the highest proliferation rates of both plums are clearly related to a drop of EtOH-soluble radioactivity of shoots by the end of subculture.
Summary Shoots of “San Castrese” and “Portici” apricots (Prunus armeniaca L.) free of cultivable... more Summary Shoots of “San Castrese” and “Portici” apricots (Prunus armeniaca L.) free of cultivable bacteria, shoots of the same origin exhibiting bacterial contamination after repeated subcultures, and contaminated shoots treated with cefotaxime were compared for gas exchange, proliferation rate, and fresh and dry weight. Cultures of San Castrese contaminated byBacillus circulans andSphingomonas paucimobilis, and of Portici contaminated withStaphylococcus hominis andMicrococcus kristinae,
Developments in Plant Pathology, 1997
... OF BACTERIAL CONTAMINANTS AND GAS EVOLUTION IN APRICOT SHOOT CULTURESGRAZIA MARINO1, NANNI ..... more ... OF BACTERIAL CONTAMINANTS AND GAS EVOLUTION IN APRICOT SHOOT CULTURESGRAZIA MARINO1, NANNI ... and cultured on proliferation media enriched with 28 and 50^ M ASA or ... 6. Magnanini, E. and Ventura, M.(1994) in'II Giornate Scientifiche SOI 1994', 22-24 ...
in Vitro Cellular & Developmental Biology-plant, 2004
The effect of different sealing materials [i.e., polyvinyl chloride (PVC) transparent film, and P... more The effect of different sealing materials [i.e., polyvinyl chloride (PVC) transparent film, and Parafilm (PARA) for Petri dishes was investigated on shoot regeneration from quince (Cydonia oblonga L.) ‘BA 29’ leaf explants. Leaves were excised from proliferating shoot cultures, transversally scored, and placed with the abaxial side down in 60-mm Petri dishes containing 10 ml of Murashige and Skoog modified medium, with 5.4 μM α-naphthaleneacetic acid, 4.5 μM thidiazuron, 200 mg l−1 cefotaxime, and 0.25% (w/v) Phytagel (IM medium) for shoot bud induction, and cultured in darkness at 22±2°C for 28 d. Then the explants were transferred to standard conditions (16-h photoperiod at 30 μmol m−2 s−1 photosynthetically active radiation) on a medium similar to IM, except for lack of NAA, and with 0.65% (w/v) agar instead of Phytagel, for an additional 15–28 d. The sealing combinations PARA-PARA, PARA-PVC, PVC-PARA, and PVC-PVC (in the induction-expression phases) were compared during regeneration and for their carry-over effect on shoot development after transfer of explants to an elongation medium (0.9 μM 6-benzyladenine). Carbon dioxide accumulated at 27.2 mmol mol−1 at the end of induction, and gradually decreased from 35.4 mmol mol−1 on day 9 to 22.5 mmol mol−1 on day 28 of the expression phase in PARA-sealed Petri dishes, being always much higher than after sealing with PVC (1–2 mmol mol−1). Ethylene concentration was 0.1 and 0.04 μmol mol−1 in the first part of the induction and expression phase, respectively, in PARA-sealed Petri dishes, and slightly decreased with duration of exposure to light during expression; while it was absent in most PVC-sealed dishes. The PARA-PARA and PVC-PVC (induction-expression) combinations gave, respectively, the worst and best results of regeneration and successive shoot development.
Plant Cell Tissue and Organ Culture, 1993
In vitro proliferation and rooting capacity of ‘San Castrese’ and ‘Portici’ apricots (Prunus arme... more In vitro proliferation and rooting capacity of ‘San Castrese’ and ‘Portici’ apricots (Prunus armeniaca L.) were tested on modified MS medium enriched with varying growth regulator concentrations and sucrose (58.4 mM) or sorbitol (116.8 mM) as main carbon energy sources. The interaction of proliferation and rooting media was also studied. Proliferation of both cultivars was proportional to benzyladenine (BA) concentration and enhanced with sorbitol media. However, 8.8 μM BA was often associated with hyperhydricity, particularly when shoots were grown on sucrose media. Newly proliferated shoots elongated better on sorbitol media. The positive influence of sorbitol on proliferation and shoot growth was not due to osmotic effects. Moreover, sorbitol showed a positive carryover effect in hastening rooting of ‘Portici’. By contrast, when transferred to sorbitol rooting media, the shoots of both cultivars generally showed low rooting, with short, thick roots. Up to 70% of the plantlets that produced roots in sucrose media enriched with indolebutyric acid were successfully acclimatized when they were dipped in a benomyl (0.075% w/v) suspension before being transplanted with care being taken to prevent over-wetting of soil.
Plant Cell, Tissue and Organ Culture, 1985
In Vitro Cellular & Developmental Biology-Plant, 1997
Summary Treatments differing from each other for the type of tube closure (ie, cotton plug for fr... more Summary Treatments differing from each other for the type of tube closure (ie, cotton plug for free gas exchange, airtight rubber cap, and rubber cap with ethysorb) and/or rooting culture medium (ie, enriched or not by 25 to 100 µM acetylsalicylic acid) were compared for their ...
… journal of plant …, 2009