Haytham Ali - Academia.edu (original) (raw)

Papers by Haytham Ali

Naunyn-Schmiedeberg's Archives of Pharmacology, 2020

The aim of this study was to investigate some biochemical indices of inflammation and oxidative a... more The aim of this study was to investigate some biochemical indices of inflammation and oxidative and nitrosative stresses in the gastrointestinal tract of mice with experimental chronic kidney disease (CKD) and treated with gum arabic (GA). Male CD1 mice (n = 28) were randomly distributed into four groups and treated for four consecutive weeks: group 1: Control: received the same diet without treatment until the end of the study; group 2: Adenine: switched to a powder diet containing adenine (0.2% w/w in feed); group 3: Gum acacia (GA): given normal feed and GA in drinking water at a concentration of 15% w/v; and group 4: Adenine + GA: given adenine in the feed as in the second group plus GA in the drinking water at concentration of 15% w/v. CKD was induced to mice by adenine feeding and concomitantly treated with the prebiotic dietary fiber gum acacia, GA (15% in drinking water). Duodenal mucosa from CKD mice had significantly higher concentrations of TNF-alfa, IL-6, and TGF-beta-1 and lipid peroxidation. Moreover, low concentrations of IL-10, some antioxidants (catalase, glutathione reductase, total antioxidant capacity, and superoxide dismutase), and nuclear factor erythroid 2-related factor 2 were found in the duodenum. The levels of nitrosative stress (nitrite, nitrate, and total nitrate) were significantly increased by CKD, as well as the concentrations of ammonia and urea creatinine in the cecal content. Concomitant GA treatment significantly mitigated these harmful effects. Taken together, GA reduces inflammation and duodenal oxidative and nitrosative stress in the gastrointestinal tract of mice with CKD.

Zagazig Veterinary Journal, 2015

Cancer is the most common cause for human death following cardiovascular disorders. The present s... more Cancer is the most common cause for human death following cardiovascular disorders. The present study was carried out to investigate the efficacy of curcumin and/or chlorambucil on modulating cancer induced by Ehrlich ascitic cells (EAC) in mice. One hundred and twenty five femal mice were equally divided into five groups. Group (1) was the normal control, groups (2-5) were injected intraperitoneally with EAC (1.4× 10 6 /0.2 ml). Group (2) was left without treatment (positive control). While groups (3-5) were treated orally with curcumin (20 mg/kg BW), chlorambucil (0.2mg/kg BW) and curcumin (20 mg/kg BW) plus chlorambucil (0.2mg/kg BW), respectively, after 48 hours from transplantation of Ehrlich cells. Blood samples and specimens from peritoneum, liver and kidney were collected from 15 mice in each group at the tenth day of the experiment. Survival analysis was carried out on the remaining animals. The obtained results revealed a significant decrease in body weight, ascetic fluids, total number of Ehrlich cells, number of live cells, serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine, blood urea nitrogen, hepatic malondialdehyde and hepatic P53 and Bax gene expression in all treated groups compared with EAC bearing mice. Moreover, the mean survival time, life span, serum levels of total proteins, albumin, albumin globulins ratio, hepatic activities of antioxidants (SOD and CAT) and hepatic Bcl-2 gene expression were increased in curcumin and/or chlorambucil treated mice compared with the non treated animals. The histopathological examination showed that the least degree of changes was in the 5 th group. In conclusion there is a synergistic good impact for using curamin as an adjuvant in combination with standard chemotherapy such as chlorambucil.

Environmental toxicology and pharmacology, Jan 3, 2018

The food additives sodium acid pyrophosphate (SAPP), sodium acetate (SA), and citric acid (CA) we... more The food additives sodium acid pyrophosphate (SAPP), sodium acetate (SA), and citric acid (CA) were evaluated for their hemato-immunotoxic effects. Forty adult Sprague-Dawley rats were distributed into four groups and were orally administered water, SAPP (12.6 mg/kg), CA (180 mg/kg), or SA (13.5 mg /kg) daily for 90 days. Erythrogram and leukogram profiles were evaluated. The levels of lysozyme, nitric oxide, immunoglobulin, and phagocytic activity were measured. Histologic and immunohistochemical evaluations of splenic tissues were performed. Changes in the mRNA expression levels of peroxisome proliferator-activated receptor α and γ (PPAR-α and PPAR-γ), and tumor necrosis factor α (TNF-α) genes were assessed. A significant leukopenic condition was observed with SAPP, while CA induced marked leukocytosis, and SA showed a lymphocytosis condition. Both the innate and humoral parameters were significantly depressed. Various pathological lesions were observed, including diffuse hyperpla...

Journal of biochemical and molecular toxicology, Jan 11, 2018

This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluori... more This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluoride (NaF) on estrogenic activity and thyroid function of adolescent female rats. The results indicated that the individual exposure to TCS evoked a significant decline in T3 and T4 but the levels of estradiol, FSH, and LH were significantly elevated beside marked up regulation of calbindin-D9k and estrogen α mRNA expression. On the other hand, the single exposure to NaF causes insignificant changes in thyroid hormones, but evoked a trend toward an increase in both estradiol and LH levels. No significant differences in the TSH level were recorded among the experimental groups. The joint exposure to TCS and NaF induced a significant improvement in thyroid and reproductive hormone levels. Overall, these findings revealed that exposure to TCS resulted in significant endocrine and reproductive alterations in immature female rats, while TCS + NaF coexposure resulted in lessening most effects.

Iranian journal of kidney diseases, 2015

Olive leaves are traditionally used in the Mediterranean basin in many medical conditions for its... more Olive leaves are traditionally used in the Mediterranean basin in many medical conditions for its potent antioxidant activity. Cyclosporine A, a well-known immunosuppressant, can induce nephrotoxicity through oxidative stress. This study investigated the effect of olive leaf extract (OLE) on cyclosporine-induced nephrotoxicity in rats. Thirty Wistar rats (180 g to 200 g) were classified into 5 groups, each containing 6 rats. The first group received normal saline and served as control. The second group was treated with cyclosporine, 25 mg/kg for 21 days for nephrotoxicity induction. Groups 3 to 5 were treated with cyclosporine, 25 mg/kg in addition to different doses of OLE (40 mg/kg, 80 mg/kg, and 120 mg/kg), respectively, for 21 days. After 21 days, the rats' body weights were recorded and the rats were sacrificed. Blood samples were collected and the animals were necropsied. Both kidneys were removed, one for histopathological and one for antioxidant activity evaluations. Cyc...

Toxicological & Environmental Chemistry, 2015

The protective effect of Spirulina platensis (SP) powder against aluminium-induced nephrotoxicity... more The protective effect of Spirulina platensis (SP) powder against aluminium-induced nephrotoxicity and DNA damage in rats was studied. Male rats receiving daily 40 mg/kg b.wt. aluminium chloride (AlCl3) orally had increased serum levels of urea and creatinine, up regulated kidney injury molecule-1 and tissue inhibitor of metalloproteinase-1 genes, down regulated catalase and glutathione peroxidase genes, and increased all parameters of kidney DNA damage using comet assay. Treatment with SP alleviated all AlCl3-induced effects of toxicity, especially when the animals were pre-treated.

Biochemical and Biophysical Research Communications, 2014

Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation inj... more Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation injury in hematopoietic stem cells with 5 Gy γ-ray exposures. We explored the effect and related mechanisms of nicaraven for protecting radiation injury induced by sequential exposures to a relatively lower dose γ-ray. C57BL/6 mice were given nicaraven or placebo within 30 minutes before exposure to 50 mGy γ-ray daily for 30 days in sequences (cumulative dose of 1.5 Gy). Mice were victimized 24 hours after the last radiation exposure, and the number, function and oxidative stress of hematopoietic stem cells were quantitatively estimated. We also compared the gene expression in these purified stem cells from mice received nicaraven and placebo treatment. Nicaraven increased the number of c-kit + stem/progenitor cells in bone marrow and peripheral blood, with a recovery rate around 60~90% of age-matched non-irradiated healthy mice. The potency of colony forming from hematopoietic stem/progenitor cells as indicator of function was completely protected with nicaraven treatment. Furthermore, nicaraven treatment changed the expression of many genes associated to DNA repair, inflammatory response, and immunomodulation in c-kit + stem/progenitor cells. Nicaraven effectively protected against damages of hematopoietic stem/progenitor cells induced by sequential exposures to a relatively low dose radiation, via complex mechanisms.

Environmental science and pollution research international, Jan 26, 2018

Our study designed to study the potential of potassium dichromate (KCrO) oral exposure to induce ... more Our study designed to study the potential of potassium dichromate (KCrO) oral exposure to induce damage in male rat brain and to compare the possible protective role of vitamin C (VC) either pre and/or concurrent supply against (KCrO) induced changes. Thirty male rats were divided into five groups. First control group received distilled water (C), second received 120 mg/kg b.wt (VC), third received 25 mg/kg b.wt KCrO(Cr), fourth group received VC together with KCrOby the same former doses (VC + Cr), and the fifth group received the same oral doses of VC 2 weeks prior to and along with KCrOfor 6 weeks (VC + Cr pro/co treated). The obtained results revealed that KCrOinduced a significant decrease in cholinergic activity, glutathione reductase GR activity, reduced glutathione content GSH and ATP levels. Furthermore, KCrOinduced a significant increase in oxidative DNA damage indicated by 8-hydroxy 2'-deoxyguanosine (8OH2'dG) and formation of apoptotic DNA ladders, significant in...

BioData Mining

BackgroundPreterm deliveries have many negative health implications on both mother and child. Ide... more BackgroundPreterm deliveries have many negative health implications on both mother and child. Identifying the population level factors that increase the risk of preterm deliveries is an important step in the direction of mitigating the impact and reducing the frequency of occurrence of preterm deliveries. The purpose of this work is to identify preterm delivery risk factors and their progression throughout the pregnancy from a large collection of Electronic Health Records (EHR).ResultsThe study cohort includes about 60,000 deliveries in the USA with the complete medical history from EHR for diagnoses, medications and procedures. We propose a temporal analysis of risk factors by estimating and comparing risk ratios and variable importance at different time points prior to the delivery event. We selected the following time points before delivery: 0, 12 and 24 week(s) of gestation. We did so by conducting a retrospective cohort study of patient history for a selected set of mothers who...

Japanese Journal of Veterinary Research, 2016

Currently, there are limited toxicological data available for fipronil exposure effect on Japanes... more Currently, there are limited toxicological data available for fipronil exposure effect on Japanese quail. The aim of the current study was to assess the toxicological effects on tissue histopathology and clinical biochemistry in a 15-day gavage study of fipronil as well as studying the physiological recovery of a 60-day depuration period after exposure in the Japanese quail. Relative liver and kidney weights in fipronil and recovery groups were non significantly changed than control. Histological changes in the liver and kidney were observed at fipronil group and recovery groups which showed restoration of the histoarchitecture particularly in an off dose 45 and 60 days groups. These changes were accompanied by clinical changes in the serum enzyme markers such as alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate transaminase (AST) and lactate dehydrogenase (LDH). Clinical biochemistry markers for kidney were not altered in all groups, except creatinine level which sh...

Archives of Disease in Childhood, 2021

Environmental Science and Pollution Research, 2020

This study was performed to assess the ability of the food genotoxicant 5-hydroxymethylfurfural (... more This study was performed to assess the ability of the food genotoxicant 5-hydroxymethylfurfural (5-HMF) to induce DNA damage and oxidative injuries in the liver of mice as a possible mechanism of toxic action and to evaluate the role of extra virgin olive oil (EVOO) in inhibiting these injuries. For this purpose, 80 mice were assigned into four equal groups of 20 mice each. Group 1 was kept as control and group 2 was given 5-HMF (250 mg/kg bw) by intraperitoneal (IP) injection 3 times weekly for 4 weeks. Group 3 received EVOO (300 μl/kg bw) orally daily for 4 weeks. Group 4 was co-treated with both 5-HMF (250 mg/kg bw) with IP injection and EVOO (300 μl/kg bw) orally 3 times weekly for 4 weeks. IP injection of 5-HMF resulted in a significant decrease in albumin, globulin, and total protein contents and significant increases in alanine transaminase, aspartate transaminase, and alkaline phosphatase activities. Administration of EVOO alone or with 5-HMF reduced the 5-HMF-induced alterations and restored the liver function biomarkers, antioxidant defense system, and histoarchitecture of the liver to normal values. EVOO also inhibited the genotoxic and apoptotic effects of 5-HMF suggesting that EVOO could provide liver protection through its powerful antioxidant and confirm its good nutriceutical and pharmacological properties.

Zagazig Veterinary Journal, 2015

Zagazig Veterinary Journal, 2019

Exosomal microRNAs (EXO-miRNAs) attract major importance as prospective diagnostic biomarkers for... more Exosomal microRNAs (EXO-miRNAs) attract major importance as prospective diagnostic biomarkers for metabolic syndrome (MS). The intent of our trials was to characterize the serum exosomes, exosomal miRNA and their target genes to identify those that were altered in relation to the different conditions of metabolic syndrome (Type II diabetes mellitus, high-fat-induced obesity and its related disorders) with different forms of treatment to evaluate their use as diagnostic and treatment biomarkers. Eighty male adult albino rats were used in this experiment, 10 served as normal control group and other 70 divided into 7 different groups; the first received high fat high fructose (HFHF) diet for 4 weeks, second received HFHF diet and confirmed to be diabetic and the other five groups are diabetics and treated for another 4 weeks with Okra extract, Gum Arabic, Dapagliflozin, combination of Dapagliflozin& okra Extract and combination of Dapagliflozin& Gum Arabic, respectively.. Our results explained that, the expression levels of miR-122, fatty acid synthase-1 (FAS-1) and Sterol response elementary binding protein-1 (SREBP-1) were significantly higher in the hepatic tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while carnitine palmitoyltransferase-1(CPT-1) was significantly decreased in the same groups. Also the expression levels of miR-31 was significantly higher in adipose tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while Leptin gene (Ob), Phosphoinositide-3-kinase, class 2, alpha-polypeptide (PIK3C2A) and Peroxisome proliferator-activated receptor-γ (PPAR-γ) were significantly decreased in the same groups.It was concluded that exosomal miRNA signatures are parallel with pathological consequences of metabolic syndrome sufferers as well as numerous miRNAs display potential biomarkers for non-invasive diagnosis of the metabolic changes.

Egyptian Journal of Histology, 2017

Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI... more Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI) proved to be less effective in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex disorder. Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced nephrotoxicity as a model of AKI. Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin injection. Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells were detected in the female kidney tissue by Y chromosome PCR technique. Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.

Scientific Reports, 2014

Effects of antioxidants on the quality and genomic stability of induced pluripotent stem (iPS) ce... more Effects of antioxidants on the quality and genomic stability of induced pluripotent stem (iPS) cells were investigated with two human iPS cell lines (201B7 and 253G1). Cells used in this study were expanded from a single colony of each cell line with the addition of proprietary antioxidant supplement or homemade antioxidant cocktail in medium, and maintained in parallel for 2 months. The cells grew well in all culture conditions and kept ''stemness''. Although antioxidants modestly decreased the levels of intracellular reactive oxygen species, there were no differences in the expression of 53BP1 and pATM, two critical molecules related with DNA damage and repair, under various culture conditions. CGH analysis showed that the events of genetic aberrations were decreased only in the 253G1 iPS cells with the addition of homemade antioxidant cocktail. Long-term culture will be necessary to confirm whether low dose antioxidants improve the quality and genomic stability of iPS cells. Results Low dose antioxidants did not affect the growth and ''stemness'' of iPS cells. We successfully maintained the iPS cell lines for 2 months by regularly passage. The shape and growth of iPS cell colonies were not obviously changed by adding either proprietary antioxidant supplement from Sigma-Aldrich (AOS) or homemade antioxidant cocktail (AOH) at relative low concentrations in culture medium for 2 months of follow-up. Immunostaining showed that all of these iPS cell colonies clearly expressed Oct3/4, Nanog, SSEA-4, and ALP

Highly pathogenic avian influenza virus (HPAIV) subtype H5N1 is circulating in Egypt since 2006, ... more Highly pathogenic avian influenza virus (HPAIV) subtype H5N1 is circulating in Egypt since 2006, with escalating apprehension about its possibility to become more transmissible amongst humans. In this study, three serial outbreaks of HPAIV H5N1 in domestic Muscovy ducks in Sharkia Governorate, Egypt were investigated. Nervous signs with 62% mortality were observed in Muscovy ducklings. Gross examination revealed severely congested meningeal vessels, hemorrhages on the duodenum, pancreas, and coronary fat. Perivascular lymphocytic cuffing, gliosis and vacuolation of the neuropil were observed in the brain microscopically. Viral antigens were identified in the neurons and the glial cells of the cerebral cortex, submucosal Meissner's plexus neurons of the intestine and the hepatic Kupffer cells by immunohistochemistry. The HPAIV subtype H5N1 was isolated from different duck tissues in 66.7% of examined duck samples. Using RAPD-PCR fingerprinting, there were different patterns in th...

International Journal of Veterinary Science and Medicine, 2018

Highly pathogenic avian influenza (HPAI) H5N1 virus poses a major challenge to the poultry indust... more Highly pathogenic avian influenza (HPAI) H5N1 virus poses a major challenge to the poultry industry and human health in Egypt. Twenty one households and eight duck farms in Sharkia Province, Egypt were investigated for the presence of avian influenza virus (AIV) and/or duck hepatitis virus 1 (DHV-1). Mortality rates among the investigated farms and yards were, 18.9% (69/365) of native ducks, 60.9% (25/41) of Pekin ducks, 60.2% (6306/10473) of Muscovy ducks and 44.9% (1353/3015) of Mallard ducks. The RT-PCR revealed the circulation of HPAI-H5N1 virus (81/104) among the examined birds with a high percentage in Muscovy (83.7%) and Pekin (83.4%) ducks. Interestingly, co-infection of HPAI and DHV-1 viruses in three ducklings with age of 4-19 days was detected. Severe neurological signs with high mortality were observed in ducklings as early as 4 days of age. Influenza virus antigen was detected in the neurons and glial cells of the brain, hepatocytes, and the intestinal submucosal plexus. Although, genetic characterization of H5N1 isolates revealed HPAIV of clade 2.2.1.2, such increased mortalities and neurological signs regardless of the duck age might imply the natural selection of HPAI in ducks. Crucial monitoring of the disease situation in ducks is essential for the implementation of an effective prevention and control program.

Avian Pathology, 2014

The highly pathogenic avian influenza virus (HPAIV) subtype H5N1 threatens animal and human healt... more The highly pathogenic avian influenza virus (HPAIV) subtype H5N1 threatens animal and human health worldwide. Susceptibility of pigeons to HPAIV (H5N1) and their role in avian influenza virus transmission to domestic birds and humans remain questionable. In this study, an outbreak in domestic pigeons (1 to 18 months old) with 50% mortality was investigated. Pigeons exhibited nervous manifestations and greenish diarrhoea. Necropsy of the naturally infected pigeons revealed congestion of the internal organs, particularly the lungs and brain. The HPAIV subtype H5N1 designated A/Pigeon/Egypt/SHAH-5803/2011 was isolated from a 40-day-old pigeon. Sequencing of the haemagglutinin gene showed it to be closely related to viruses in group 2.2.1/C. Intravenous inoculation of the isolate in chickens induced 100% mortality within 2 days post inoculation and the intravenous pathogenicity index was 2.7. Virus pathogenicity and transmissibility was determined experimentally in 6-week-old domestic pigeons. Thirty per cent of pigeons inoculated oronasally with 10(6) median embryo infective dose showed congested beak, conjunctivitis, depression, and greenish diarrhoea. A mortality rate of 10% was recorded preceded by severe neurologic signs consisting of torticollis, incoordination, tremors, and wing paralysis. Pathological examination revealed a friable brain tissue and congested meningeal blood vessels. The lungs appeared oedematous and severely haemorrhagic. Subepicardial and petechial haemorrhages on the coronary fat were observed. Both infected and contact pigeons shed virus via the oropharynx and cloaca. To our knowledge, this is the first description and characterization of HPAIV in naturally infected pigeons in Egypt. Our findings reveal that pigeons can indeed be susceptible to H5N1 HPAIVs and could be a source of infection to other birds and humans.

Naunyn-Schmiedeberg's Archives of Pharmacology, 2020

The aim of this study was to investigate some biochemical indices of inflammation and oxidative a... more The aim of this study was to investigate some biochemical indices of inflammation and oxidative and nitrosative stresses in the gastrointestinal tract of mice with experimental chronic kidney disease (CKD) and treated with gum arabic (GA). Male CD1 mice (n = 28) were randomly distributed into four groups and treated for four consecutive weeks: group 1: Control: received the same diet without treatment until the end of the study; group 2: Adenine: switched to a powder diet containing adenine (0.2% w/w in feed); group 3: Gum acacia (GA): given normal feed and GA in drinking water at a concentration of 15% w/v; and group 4: Adenine + GA: given adenine in the feed as in the second group plus GA in the drinking water at concentration of 15% w/v. CKD was induced to mice by adenine feeding and concomitantly treated with the prebiotic dietary fiber gum acacia, GA (15% in drinking water). Duodenal mucosa from CKD mice had significantly higher concentrations of TNF-alfa, IL-6, and TGF-beta-1 and lipid peroxidation. Moreover, low concentrations of IL-10, some antioxidants (catalase, glutathione reductase, total antioxidant capacity, and superoxide dismutase), and nuclear factor erythroid 2-related factor 2 were found in the duodenum. The levels of nitrosative stress (nitrite, nitrate, and total nitrate) were significantly increased by CKD, as well as the concentrations of ammonia and urea creatinine in the cecal content. Concomitant GA treatment significantly mitigated these harmful effects. Taken together, GA reduces inflammation and duodenal oxidative and nitrosative stress in the gastrointestinal tract of mice with CKD.

Zagazig Veterinary Journal, 2015

Cancer is the most common cause for human death following cardiovascular disorders. The present s... more Cancer is the most common cause for human death following cardiovascular disorders. The present study was carried out to investigate the efficacy of curcumin and/or chlorambucil on modulating cancer induced by Ehrlich ascitic cells (EAC) in mice. One hundred and twenty five femal mice were equally divided into five groups. Group (1) was the normal control, groups (2-5) were injected intraperitoneally with EAC (1.4× 10 6 /0.2 ml). Group (2) was left without treatment (positive control). While groups (3-5) were treated orally with curcumin (20 mg/kg BW), chlorambucil (0.2mg/kg BW) and curcumin (20 mg/kg BW) plus chlorambucil (0.2mg/kg BW), respectively, after 48 hours from transplantation of Ehrlich cells. Blood samples and specimens from peritoneum, liver and kidney were collected from 15 mice in each group at the tenth day of the experiment. Survival analysis was carried out on the remaining animals. The obtained results revealed a significant decrease in body weight, ascetic fluids, total number of Ehrlich cells, number of live cells, serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine, blood urea nitrogen, hepatic malondialdehyde and hepatic P53 and Bax gene expression in all treated groups compared with EAC bearing mice. Moreover, the mean survival time, life span, serum levels of total proteins, albumin, albumin globulins ratio, hepatic activities of antioxidants (SOD and CAT) and hepatic Bcl-2 gene expression were increased in curcumin and/or chlorambucil treated mice compared with the non treated animals. The histopathological examination showed that the least degree of changes was in the 5 th group. In conclusion there is a synergistic good impact for using curamin as an adjuvant in combination with standard chemotherapy such as chlorambucil.

Environmental toxicology and pharmacology, Jan 3, 2018

The food additives sodium acid pyrophosphate (SAPP), sodium acetate (SA), and citric acid (CA) we... more The food additives sodium acid pyrophosphate (SAPP), sodium acetate (SA), and citric acid (CA) were evaluated for their hemato-immunotoxic effects. Forty adult Sprague-Dawley rats were distributed into four groups and were orally administered water, SAPP (12.6 mg/kg), CA (180 mg/kg), or SA (13.5 mg /kg) daily for 90 days. Erythrogram and leukogram profiles were evaluated. The levels of lysozyme, nitric oxide, immunoglobulin, and phagocytic activity were measured. Histologic and immunohistochemical evaluations of splenic tissues were performed. Changes in the mRNA expression levels of peroxisome proliferator-activated receptor α and γ (PPAR-α and PPAR-γ), and tumor necrosis factor α (TNF-α) genes were assessed. A significant leukopenic condition was observed with SAPP, while CA induced marked leukocytosis, and SA showed a lymphocytosis condition. Both the innate and humoral parameters were significantly depressed. Various pathological lesions were observed, including diffuse hyperpla...

Journal of biochemical and molecular toxicology, Jan 11, 2018

This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluori... more This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluoride (NaF) on estrogenic activity and thyroid function of adolescent female rats. The results indicated that the individual exposure to TCS evoked a significant decline in T3 and T4 but the levels of estradiol, FSH, and LH were significantly elevated beside marked up regulation of calbindin-D9k and estrogen α mRNA expression. On the other hand, the single exposure to NaF causes insignificant changes in thyroid hormones, but evoked a trend toward an increase in both estradiol and LH levels. No significant differences in the TSH level were recorded among the experimental groups. The joint exposure to TCS and NaF induced a significant improvement in thyroid and reproductive hormone levels. Overall, these findings revealed that exposure to TCS resulted in significant endocrine and reproductive alterations in immature female rats, while TCS + NaF coexposure resulted in lessening most effects.

Iranian journal of kidney diseases, 2015

Olive leaves are traditionally used in the Mediterranean basin in many medical conditions for its... more Olive leaves are traditionally used in the Mediterranean basin in many medical conditions for its potent antioxidant activity. Cyclosporine A, a well-known immunosuppressant, can induce nephrotoxicity through oxidative stress. This study investigated the effect of olive leaf extract (OLE) on cyclosporine-induced nephrotoxicity in rats. Thirty Wistar rats (180 g to 200 g) were classified into 5 groups, each containing 6 rats. The first group received normal saline and served as control. The second group was treated with cyclosporine, 25 mg/kg for 21 days for nephrotoxicity induction. Groups 3 to 5 were treated with cyclosporine, 25 mg/kg in addition to different doses of OLE (40 mg/kg, 80 mg/kg, and 120 mg/kg), respectively, for 21 days. After 21 days, the rats' body weights were recorded and the rats were sacrificed. Blood samples were collected and the animals were necropsied. Both kidneys were removed, one for histopathological and one for antioxidant activity evaluations. Cyc...

Toxicological & Environmental Chemistry, 2015

The protective effect of Spirulina platensis (SP) powder against aluminium-induced nephrotoxicity... more The protective effect of Spirulina platensis (SP) powder against aluminium-induced nephrotoxicity and DNA damage in rats was studied. Male rats receiving daily 40 mg/kg b.wt. aluminium chloride (AlCl3) orally had increased serum levels of urea and creatinine, up regulated kidney injury molecule-1 and tissue inhibitor of metalloproteinase-1 genes, down regulated catalase and glutathione peroxidase genes, and increased all parameters of kidney DNA damage using comet assay. Treatment with SP alleviated all AlCl3-induced effects of toxicity, especially when the animals were pre-treated.

Biochemical and Biophysical Research Communications, 2014

Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation inj... more Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation injury in hematopoietic stem cells with 5 Gy γ-ray exposures. We explored the effect and related mechanisms of nicaraven for protecting radiation injury induced by sequential exposures to a relatively lower dose γ-ray. C57BL/6 mice were given nicaraven or placebo within 30 minutes before exposure to 50 mGy γ-ray daily for 30 days in sequences (cumulative dose of 1.5 Gy). Mice were victimized 24 hours after the last radiation exposure, and the number, function and oxidative stress of hematopoietic stem cells were quantitatively estimated. We also compared the gene expression in these purified stem cells from mice received nicaraven and placebo treatment. Nicaraven increased the number of c-kit + stem/progenitor cells in bone marrow and peripheral blood, with a recovery rate around 60~90% of age-matched non-irradiated healthy mice. The potency of colony forming from hematopoietic stem/progenitor cells as indicator of function was completely protected with nicaraven treatment. Furthermore, nicaraven treatment changed the expression of many genes associated to DNA repair, inflammatory response, and immunomodulation in c-kit + stem/progenitor cells. Nicaraven effectively protected against damages of hematopoietic stem/progenitor cells induced by sequential exposures to a relatively low dose radiation, via complex mechanisms.

Environmental science and pollution research international, Jan 26, 2018

Our study designed to study the potential of potassium dichromate (KCrO) oral exposure to induce ... more Our study designed to study the potential of potassium dichromate (KCrO) oral exposure to induce damage in male rat brain and to compare the possible protective role of vitamin C (VC) either pre and/or concurrent supply against (KCrO) induced changes. Thirty male rats were divided into five groups. First control group received distilled water (C), second received 120 mg/kg b.wt (VC), third received 25 mg/kg b.wt KCrO(Cr), fourth group received VC together with KCrOby the same former doses (VC + Cr), and the fifth group received the same oral doses of VC 2 weeks prior to and along with KCrOfor 6 weeks (VC + Cr pro/co treated). The obtained results revealed that KCrOinduced a significant decrease in cholinergic activity, glutathione reductase GR activity, reduced glutathione content GSH and ATP levels. Furthermore, KCrOinduced a significant increase in oxidative DNA damage indicated by 8-hydroxy 2'-deoxyguanosine (8OH2'dG) and formation of apoptotic DNA ladders, significant in...

BioData Mining

BackgroundPreterm deliveries have many negative health implications on both mother and child. Ide... more BackgroundPreterm deliveries have many negative health implications on both mother and child. Identifying the population level factors that increase the risk of preterm deliveries is an important step in the direction of mitigating the impact and reducing the frequency of occurrence of preterm deliveries. The purpose of this work is to identify preterm delivery risk factors and their progression throughout the pregnancy from a large collection of Electronic Health Records (EHR).ResultsThe study cohort includes about 60,000 deliveries in the USA with the complete medical history from EHR for diagnoses, medications and procedures. We propose a temporal analysis of risk factors by estimating and comparing risk ratios and variable importance at different time points prior to the delivery event. We selected the following time points before delivery: 0, 12 and 24 week(s) of gestation. We did so by conducting a retrospective cohort study of patient history for a selected set of mothers who...

Japanese Journal of Veterinary Research, 2016

Currently, there are limited toxicological data available for fipronil exposure effect on Japanes... more Currently, there are limited toxicological data available for fipronil exposure effect on Japanese quail. The aim of the current study was to assess the toxicological effects on tissue histopathology and clinical biochemistry in a 15-day gavage study of fipronil as well as studying the physiological recovery of a 60-day depuration period after exposure in the Japanese quail. Relative liver and kidney weights in fipronil and recovery groups were non significantly changed than control. Histological changes in the liver and kidney were observed at fipronil group and recovery groups which showed restoration of the histoarchitecture particularly in an off dose 45 and 60 days groups. These changes were accompanied by clinical changes in the serum enzyme markers such as alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate transaminase (AST) and lactate dehydrogenase (LDH). Clinical biochemistry markers for kidney were not altered in all groups, except creatinine level which sh...

Archives of Disease in Childhood, 2021

Environmental Science and Pollution Research, 2020

This study was performed to assess the ability of the food genotoxicant 5-hydroxymethylfurfural (... more This study was performed to assess the ability of the food genotoxicant 5-hydroxymethylfurfural (5-HMF) to induce DNA damage and oxidative injuries in the liver of mice as a possible mechanism of toxic action and to evaluate the role of extra virgin olive oil (EVOO) in inhibiting these injuries. For this purpose, 80 mice were assigned into four equal groups of 20 mice each. Group 1 was kept as control and group 2 was given 5-HMF (250 mg/kg bw) by intraperitoneal (IP) injection 3 times weekly for 4 weeks. Group 3 received EVOO (300 μl/kg bw) orally daily for 4 weeks. Group 4 was co-treated with both 5-HMF (250 mg/kg bw) with IP injection and EVOO (300 μl/kg bw) orally 3 times weekly for 4 weeks. IP injection of 5-HMF resulted in a significant decrease in albumin, globulin, and total protein contents and significant increases in alanine transaminase, aspartate transaminase, and alkaline phosphatase activities. Administration of EVOO alone or with 5-HMF reduced the 5-HMF-induced alterations and restored the liver function biomarkers, antioxidant defense system, and histoarchitecture of the liver to normal values. EVOO also inhibited the genotoxic and apoptotic effects of 5-HMF suggesting that EVOO could provide liver protection through its powerful antioxidant and confirm its good nutriceutical and pharmacological properties.

Zagazig Veterinary Journal, 2015

Zagazig Veterinary Journal, 2019

Exosomal microRNAs (EXO-miRNAs) attract major importance as prospective diagnostic biomarkers for... more Exosomal microRNAs (EXO-miRNAs) attract major importance as prospective diagnostic biomarkers for metabolic syndrome (MS). The intent of our trials was to characterize the serum exosomes, exosomal miRNA and their target genes to identify those that were altered in relation to the different conditions of metabolic syndrome (Type II diabetes mellitus, high-fat-induced obesity and its related disorders) with different forms of treatment to evaluate their use as diagnostic and treatment biomarkers. Eighty male adult albino rats were used in this experiment, 10 served as normal control group and other 70 divided into 7 different groups; the first received high fat high fructose (HFHF) diet for 4 weeks, second received HFHF diet and confirmed to be diabetic and the other five groups are diabetics and treated for another 4 weeks with Okra extract, Gum Arabic, Dapagliflozin, combination of Dapagliflozin& okra Extract and combination of Dapagliflozin& Gum Arabic, respectively.. Our results explained that, the expression levels of miR-122, fatty acid synthase-1 (FAS-1) and Sterol response elementary binding protein-1 (SREBP-1) were significantly higher in the hepatic tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while carnitine palmitoyltransferase-1(CPT-1) was significantly decreased in the same groups. Also the expression levels of miR-31 was significantly higher in adipose tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while Leptin gene (Ob), Phosphoinositide-3-kinase, class 2, alpha-polypeptide (PIK3C2A) and Peroxisome proliferator-activated receptor-γ (PPAR-γ) were significantly decreased in the same groups.It was concluded that exosomal miRNA signatures are parallel with pathological consequences of metabolic syndrome sufferers as well as numerous miRNAs display potential biomarkers for non-invasive diagnosis of the metabolic changes.

Egyptian Journal of Histology, 2017

Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI... more Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI) proved to be less effective in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex disorder. Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced nephrotoxicity as a model of AKI. Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin injection. Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells were detected in the female kidney tissue by Y chromosome PCR technique. Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.

Scientific Reports, 2014

Effects of antioxidants on the quality and genomic stability of induced pluripotent stem (iPS) ce... more Effects of antioxidants on the quality and genomic stability of induced pluripotent stem (iPS) cells were investigated with two human iPS cell lines (201B7 and 253G1). Cells used in this study were expanded from a single colony of each cell line with the addition of proprietary antioxidant supplement or homemade antioxidant cocktail in medium, and maintained in parallel for 2 months. The cells grew well in all culture conditions and kept ''stemness''. Although antioxidants modestly decreased the levels of intracellular reactive oxygen species, there were no differences in the expression of 53BP1 and pATM, two critical molecules related with DNA damage and repair, under various culture conditions. CGH analysis showed that the events of genetic aberrations were decreased only in the 253G1 iPS cells with the addition of homemade antioxidant cocktail. Long-term culture will be necessary to confirm whether low dose antioxidants improve the quality and genomic stability of iPS cells. Results Low dose antioxidants did not affect the growth and ''stemness'' of iPS cells. We successfully maintained the iPS cell lines for 2 months by regularly passage. The shape and growth of iPS cell colonies were not obviously changed by adding either proprietary antioxidant supplement from Sigma-Aldrich (AOS) or homemade antioxidant cocktail (AOH) at relative low concentrations in culture medium for 2 months of follow-up. Immunostaining showed that all of these iPS cell colonies clearly expressed Oct3/4, Nanog, SSEA-4, and ALP

Highly pathogenic avian influenza virus (HPAIV) subtype H5N1 is circulating in Egypt since 2006, ... more Highly pathogenic avian influenza virus (HPAIV) subtype H5N1 is circulating in Egypt since 2006, with escalating apprehension about its possibility to become more transmissible amongst humans. In this study, three serial outbreaks of HPAIV H5N1 in domestic Muscovy ducks in Sharkia Governorate, Egypt were investigated. Nervous signs with 62% mortality were observed in Muscovy ducklings. Gross examination revealed severely congested meningeal vessels, hemorrhages on the duodenum, pancreas, and coronary fat. Perivascular lymphocytic cuffing, gliosis and vacuolation of the neuropil were observed in the brain microscopically. Viral antigens were identified in the neurons and the glial cells of the cerebral cortex, submucosal Meissner's plexus neurons of the intestine and the hepatic Kupffer cells by immunohistochemistry. The HPAIV subtype H5N1 was isolated from different duck tissues in 66.7% of examined duck samples. Using RAPD-PCR fingerprinting, there were different patterns in th...

International Journal of Veterinary Science and Medicine, 2018

Highly pathogenic avian influenza (HPAI) H5N1 virus poses a major challenge to the poultry indust... more Highly pathogenic avian influenza (HPAI) H5N1 virus poses a major challenge to the poultry industry and human health in Egypt. Twenty one households and eight duck farms in Sharkia Province, Egypt were investigated for the presence of avian influenza virus (AIV) and/or duck hepatitis virus 1 (DHV-1). Mortality rates among the investigated farms and yards were, 18.9% (69/365) of native ducks, 60.9% (25/41) of Pekin ducks, 60.2% (6306/10473) of Muscovy ducks and 44.9% (1353/3015) of Mallard ducks. The RT-PCR revealed the circulation of HPAI-H5N1 virus (81/104) among the examined birds with a high percentage in Muscovy (83.7%) and Pekin (83.4%) ducks. Interestingly, co-infection of HPAI and DHV-1 viruses in three ducklings with age of 4-19 days was detected. Severe neurological signs with high mortality were observed in ducklings as early as 4 days of age. Influenza virus antigen was detected in the neurons and glial cells of the brain, hepatocytes, and the intestinal submucosal plexus. Although, genetic characterization of H5N1 isolates revealed HPAIV of clade 2.2.1.2, such increased mortalities and neurological signs regardless of the duck age might imply the natural selection of HPAI in ducks. Crucial monitoring of the disease situation in ducks is essential for the implementation of an effective prevention and control program.

Avian Pathology, 2014

The highly pathogenic avian influenza virus (HPAIV) subtype H5N1 threatens animal and human healt... more The highly pathogenic avian influenza virus (HPAIV) subtype H5N1 threatens animal and human health worldwide. Susceptibility of pigeons to HPAIV (H5N1) and their role in avian influenza virus transmission to domestic birds and humans remain questionable. In this study, an outbreak in domestic pigeons (1 to 18 months old) with 50% mortality was investigated. Pigeons exhibited nervous manifestations and greenish diarrhoea. Necropsy of the naturally infected pigeons revealed congestion of the internal organs, particularly the lungs and brain. The HPAIV subtype H5N1 designated A/Pigeon/Egypt/SHAH-5803/2011 was isolated from a 40-day-old pigeon. Sequencing of the haemagglutinin gene showed it to be closely related to viruses in group 2.2.1/C. Intravenous inoculation of the isolate in chickens induced 100% mortality within 2 days post inoculation and the intravenous pathogenicity index was 2.7. Virus pathogenicity and transmissibility was determined experimentally in 6-week-old domestic pigeons. Thirty per cent of pigeons inoculated oronasally with 10(6) median embryo infective dose showed congested beak, conjunctivitis, depression, and greenish diarrhoea. A mortality rate of 10% was recorded preceded by severe neurologic signs consisting of torticollis, incoordination, tremors, and wing paralysis. Pathological examination revealed a friable brain tissue and congested meningeal blood vessels. The lungs appeared oedematous and severely haemorrhagic. Subepicardial and petechial haemorrhages on the coronary fat were observed. Both infected and contact pigeons shed virus via the oropharynx and cloaca. To our knowledge, this is the first description and characterization of HPAIV in naturally infected pigeons in Egypt. Our findings reveal that pigeons can indeed be susceptible to H5N1 HPAIVs and could be a source of infection to other birds and humans.