Huynh Vu - Academia.edu (original) (raw)
Papers by Huynh Vu
2017 International Conference on Recent Advances in Signal Processing, Telecommunications & Computing (SigTelCom), 2017
The recent application of Convolutional Neural Networks (CNNs) to Human Pose Estimation (HPE) fro... more The recent application of Convolutional Neural Networks (CNNs) to Human Pose Estimation (HPE) from static images have improved estimation accuracy compared to traditional HPE approaches. In particular, a recent novel HPE approach combines a traditional graphical model with CNNs to result in state-of-the-art HPE accuracy, improving the estimation accuracy compared to using either approach alone. However, the accuracy of the CNN used in the hybrid model has not yet been explored, and this paper evaluates the use of CNNs in the hybrid model through investigating different network configurations and fine-tuning the network using pre-trained weights obtained from a large labeled dataset. The proposed CNN configurations not only improve the accuracy of the existing network by up to 2% but also uses fewer parameters, resulting in a higher HPE accuracy and simpler network structure.
Nucleic acids symposium series, 1990
The optimal system for the rapid, efficient, convenient, and economical synthesis and purificatio... more The optimal system for the rapid, efficient, convenient, and economical synthesis and purification of synthetic oligonucleotides has been advancing. By recognizing the very rapid reaction kinetics and taking advantage of an efficient, low volume delivery system, cycle times have decreased to about 5.5 minutes, without compromising synthesis performance. A new set of base protecting groups for cyanoethylphosphoramidite nucleoside monomers have been developed, which decreases the post-synthesis time requirements. A particular form of polystyrene has also been developed as a solid support for automated oligonucleotide synthesis. Typical sequencing or PCR primers (20mers) now require less than 2 hours for synthesis and 2 hours for cleavage and deprotection.
Nucleosides and Nucleotides, 1993
Preparation of G-rich TFOs containing cholesterol at the end presents challenging problems due to... more Preparation of G-rich TFOs containing cholesterol at the end presents challenging problems due to low yield in synthesis and loss in purification due to aggregation. These compounds also have low solubility which can result in variability in their uptake properties and cellular efficacy. To overcome these problems we have designed and synthesized cholesteryl -CPG (6) and -TentaGel (7) supports and phosphoramidite (8) containing a triglycyl linker.
Tetrahedron Letters, 1990
![Research paper thumbnail of Corrigendum to “The use of 6-carboxynaphthofluorescein phosphoramidite in the automated synthesis of quencher-dye oligonucleotide probes (QDOPs)” [Tetrahedron Lett. 50 (2009) 737]](https://attachments.academia-assets.com/93509629/thumbnails/1.jpg)
](Tetrahedron Letters, 2009
Corrigendum to ''The use of 6-carboxynaphthofluorescein phosphoramidite in the automated synthesi... more Corrigendum to ''The use of 6-carboxynaphthofluorescein phosphoramidite in the automated synthesis of quencher-dye oligonucleotide probes (QDOPs)" [Tetrahedron Lett. 50 (2009) 737]
Tetrahedron Letters, 2009
Real-time detection and quantitation of specific amplicons have been achieved using quencher and ... more Real-time detection and quantitation of specific amplicons have been achieved using quencher and dyelabeled oligonucleotides such as molecular beacons. The molecular beacon molecule has a fluorescent reporter dye at the 5 0-end and a quencher at the 3 0-end. When the closed molecular beacon is excited by irradiation, the reporter fluorescence is greatly reduced by quenching through the process of fluorescence resonance energy transfer. When the molecular beacon hybridizes to the target, the stem loop opens making the fluorophore and quencher spatially distinct, thus increasing the reporter dye fluorescence intensity. Labeling of dyes to 5 0-end of oligonucleotides has been done typically using manual methods, it is possible to do manual coupling at the milligram scale. Described here is the development of a scalable process for oligonucleotide labeling, which is robust, and has been achieved for 6-carboxynaphthofluorescein by connecting it to a corresponding phosphoramidite [
Nucleic Acids Research, 1993
An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides ... more An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides which have been connected by a flexible polymeric linker chain. As a prototype, binding of this class of oligonucleotide to duplex DNA has been studied using a segment of the HSV-1 D-glycoprotein promoter, which possesses a pair of 12bp target sites for stable triple helix formation, separated by a duplex spacer region which is one helical tum long. Band shift and footprinting analysis show that such hybrids can bind to both 12bp elements simultaneously, if flexible linkers are Included which are longer than 20-25 rotatable bonds. Molecular modeling confirms that a flexible polymeric linker as short as 22 rotatable bonds is enough to link the two distant segments of triple helix, providing that the linker element travels a path which Is external to the helix grooves and parallel to the long helix axis.
Bioconjugate Chemistry, 1994
![Research paper thumbnail of Use of Phthaloyl Protecting Group for the Automated Synthesis of 3'-[(Hydroxypropyl)amino] and 3'-[(Hydroxypropyltriglycyl] Oligonucleotide Conjugates](https://attachments.academia-assets.com/93509633/thumbnails/1.jpg)
](Bioconjugate Chemistry, 1995
The chemical stability of oligonucleotides (ODNs) containing 3'-propanolamine was investigated. I... more The chemical stability of oligonucleotides (ODNs) containing 3'-propanolamine was investigated. Invariably, all the ODNs synthesized from Fmoc-protected 3-aminopropane-1,2-diol-CPG support gave a mixture of three compounds at the end of automated synthesis as analyzed by denaturing PAGE and HPLC. On the basis of analytical procedures, these compounds were identified to be 3'-[N-acetyl-N-(hydroxypropyl)amino], 3'-[(hydroxypropyl)amino], and 3'-hydroxyl ODNs. The instability of the amino protecting group under the synthesis conditions was responsible for this observed heterogeneity. In order to evaluate the stability, a comparative study on the chemical stability of the ODN containing amino-protecting groups such as [(9-fluorenylmethyl)oxylcarbonyl (Fmoc), trifluoroacetyl (TFA), and phthaloyl was undertaken. The results indicate that the phthaloyl group provided the best stability for the synthesis of 3' amine-modified ODNs, and the protecting group is cleaved and deprotected in concentrated ammonium hydroxide:40% aqueous methylamine, 1: 1, for 5-10 min, at 56 O C. The 3'-[(hydroxypropyl)triglycyll ODN conjugates were also synthesized from Fmoc-and phthaloyl-protected (hydroxypropy1)triglycine-CPG supports.
2017 International Conference on Recent Advances in Signal Processing, Telecommunications & Computing (SigTelCom), 2017
The recent application of Convolutional Neural Networks (CNNs) to Human Pose Estimation (HPE) fro... more The recent application of Convolutional Neural Networks (CNNs) to Human Pose Estimation (HPE) from static images have improved estimation accuracy compared to traditional HPE approaches. In particular, a recent novel HPE approach combines a traditional graphical model with CNNs to result in state-of-the-art HPE accuracy, improving the estimation accuracy compared to using either approach alone. However, the accuracy of the CNN used in the hybrid model has not yet been explored, and this paper evaluates the use of CNNs in the hybrid model through investigating different network configurations and fine-tuning the network using pre-trained weights obtained from a large labeled dataset. The proposed CNN configurations not only improve the accuracy of the existing network by up to 2% but also uses fewer parameters, resulting in a higher HPE accuracy and simpler network structure.
Nucleic acids symposium series, 1990
The optimal system for the rapid, efficient, convenient, and economical synthesis and purificatio... more The optimal system for the rapid, efficient, convenient, and economical synthesis and purification of synthetic oligonucleotides has been advancing. By recognizing the very rapid reaction kinetics and taking advantage of an efficient, low volume delivery system, cycle times have decreased to about 5.5 minutes, without compromising synthesis performance. A new set of base protecting groups for cyanoethylphosphoramidite nucleoside monomers have been developed, which decreases the post-synthesis time requirements. A particular form of polystyrene has also been developed as a solid support for automated oligonucleotide synthesis. Typical sequencing or PCR primers (20mers) now require less than 2 hours for synthesis and 2 hours for cleavage and deprotection.
Nucleosides and Nucleotides, 1993
Preparation of G-rich TFOs containing cholesterol at the end presents challenging problems due to... more Preparation of G-rich TFOs containing cholesterol at the end presents challenging problems due to low yield in synthesis and loss in purification due to aggregation. These compounds also have low solubility which can result in variability in their uptake properties and cellular efficacy. To overcome these problems we have designed and synthesized cholesteryl -CPG (6) and -TentaGel (7) supports and phosphoramidite (8) containing a triglycyl linker.
Tetrahedron Letters, 1990
Tetrahedron Letters, 2009
Corrigendum to ''The use of 6-carboxynaphthofluorescein phosphoramidite in the automated synthesi... more Corrigendum to ''The use of 6-carboxynaphthofluorescein phosphoramidite in the automated synthesis of quencher-dye oligonucleotide probes (QDOPs)" [Tetrahedron Lett. 50 (2009) 737]
Tetrahedron Letters, 2009
Real-time detection and quantitation of specific amplicons have been achieved using quencher and ... more Real-time detection and quantitation of specific amplicons have been achieved using quencher and dyelabeled oligonucleotides such as molecular beacons. The molecular beacon molecule has a fluorescent reporter dye at the 5 0-end and a quencher at the 3 0-end. When the closed molecular beacon is excited by irradiation, the reporter fluorescence is greatly reduced by quenching through the process of fluorescence resonance energy transfer. When the molecular beacon hybridizes to the target, the stem loop opens making the fluorophore and quencher spatially distinct, thus increasing the reporter dye fluorescence intensity. Labeling of dyes to 5 0-end of oligonucleotides has been done typically using manual methods, it is possible to do manual coupling at the milligram scale. Described here is the development of a scalable process for oligonucleotide labeling, which is robust, and has been achieved for 6-carboxynaphthofluorescein by connecting it to a corresponding phosphoramidite [
Nucleic Acids Research, 1993
An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides ... more An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides which have been connected by a flexible polymeric linker chain. As a prototype, binding of this class of oligonucleotide to duplex DNA has been studied using a segment of the HSV-1 D-glycoprotein promoter, which possesses a pair of 12bp target sites for stable triple helix formation, separated by a duplex spacer region which is one helical tum long. Band shift and footprinting analysis show that such hybrids can bind to both 12bp elements simultaneously, if flexible linkers are Included which are longer than 20-25 rotatable bonds. Molecular modeling confirms that a flexible polymeric linker as short as 22 rotatable bonds is enough to link the two distant segments of triple helix, providing that the linker element travels a path which Is external to the helix grooves and parallel to the long helix axis.
Bioconjugate Chemistry, 1994
Bioconjugate Chemistry, 1995
The chemical stability of oligonucleotides (ODNs) containing 3'-propanolamine was investigated. I... more The chemical stability of oligonucleotides (ODNs) containing 3'-propanolamine was investigated. Invariably, all the ODNs synthesized from Fmoc-protected 3-aminopropane-1,2-diol-CPG support gave a mixture of three compounds at the end of automated synthesis as analyzed by denaturing PAGE and HPLC. On the basis of analytical procedures, these compounds were identified to be 3'-[N-acetyl-N-(hydroxypropyl)amino], 3'-[(hydroxypropyl)amino], and 3'-hydroxyl ODNs. The instability of the amino protecting group under the synthesis conditions was responsible for this observed heterogeneity. In order to evaluate the stability, a comparative study on the chemical stability of the ODN containing amino-protecting groups such as [(9-fluorenylmethyl)oxylcarbonyl (Fmoc), trifluoroacetyl (TFA), and phthaloyl was undertaken. The results indicate that the phthaloyl group provided the best stability for the synthesis of 3' amine-modified ODNs, and the protecting group is cleaved and deprotected in concentrated ammonium hydroxide:40% aqueous methylamine, 1: 1, for 5-10 min, at 56 O C. The 3'-[(hydroxypropyl)triglycyll ODN conjugates were also synthesized from Fmoc-and phthaloyl-protected (hydroxypropy1)triglycine-CPG supports.