Imran ullah - Academia.edu (original) (raw)

Papers by Imran ullah

Advances in Entomology, 2021

Cabbage white butterfly (Pieris brassicae) is one of the important insect pests of cabbage crop w... more Cabbage white butterfly (Pieris brassicae) is one of the important insect pests of cabbage crop which causes remarkable quantitative or qualitative crop losses. The research was conducted at Hazara Agricultural research station, Abbottabad (Pakistan) to study the effect of different new chemical insecticides, botanical oil and neem seed oil (Azadirachta indica A. Juss.) on the population density of P. brassicae. The study showed that neem oil had a significant effect on population of P. brassicae in comparison to control treatment. So, neem oil alone or in combination with insecticides can be used for control of P. brassicae in vegetable crops for a safer food supply.

GJMS Vol 18, No.2, Apr-Jun 2020, 2020

Background: Escherichia coliresistance to ceftriaxone in UTIs is an emerging health problem.Our o... more Background: Escherichia coliresistance to ceftriaxone in UTIs is an emerging health problem.Our objectives were to determine prevalence, distribution and determinants of E. coliresistance to ceftriaxone in adult indoor UTI population of District Peshawar, Pakistan. Materials & Methods:This cross-sectional study was conducted in Department of Medicine, Khyber Teaching Hospital, Peshawar, Pakistan from 1st January 2017 to 30th June 2017. 380 UTIs cases were selected from population at riskconsecutively.Sex and age groups were demographic, while presence of E. coli resistance to ceftriaxone was research variable. All variables were nominal.Prevalenceand distribution were analyzed by count, percentage and confidence intervals for proportion for population. Hypotheses for distribution were substantiated by chi-square goodness-of-fit and of association by chi-square test of association. Results: Out of 380 patients with UTI, 136 (35.80%) were men,244(64.20%) women, 262 (68.95%) in age gro...

Animal Cells and Systems, 2020

the differentiation of porcine bone marrow mesenchymal stem cells into premature hepatocyte-like ... more the differentiation of porcine bone marrow mesenchymal stem cells into premature hepatocyte-like cells in an indirect coculture system with primary hepatocytes,

Supplemental Methods (Synthesis Methods) (PDF) Morphological changes in Leishmania promastigotes ... more Supplemental Methods (Synthesis Methods) (PDF) Morphological changes in Leishmania promastigotes treated with MMV676477 and analogs, effect of paclitaxel on porcine microtubule assembly in vitro, relative polymerization activity of MMV676477 in presence or absence of detergent, effect of miltefosine on purified tubulin assembly in vitro, relative polymerization activity of MMV676477 in the presence or absence of detergent, fluorescent images of purified tubulin treated with paclitaxel and MMV676477, full fluorescence and Coomassie blue gels from Figure 6, competition-sensitive fluorescent binding of MMV676477 analogs to tubulin and other proteins, screening of the MMV Pathogen Box, and cytotoxicity data (CC 50 values) for selected "hit" compounds. (PDF)

Journal of Animal Reproduction and Biotechnology, 2017

Numerical Methods for Partial Differential Equations, 2017

The hydromagnetic mixed convection flow of Casson fluid due to moving wedge embedded in a porous ... more The hydromagnetic mixed convection flow of Casson fluid due to moving wedge embedded in a porous medium in the presence of chemical reaction and viscous dissipation is investigated. The Joule heating due to a magnetic field and porous medium heating is also considered. Similarity transformations are utilized to convert nonlinear partial differential equations into nonlinear ordinary differential equations. The resulting equations are solved numerically via Keller-box method. The numerical results are achieved for limiting cases and are revealed in close agreement with those of the results available in the literature. It is noticed that unsteadiness parameter thinning the velocity boundary layer while opposite to this was found in the thermal and concentration boundary layers. The dimensionless temperature is observed to be enhanced with increment in Eckert number.

Malaysian Journal of Fundamental and Applied Sciences, 2017

The problem of heat and mass transfer free convection flow of Casson fluid over a porous nonlinea... more The problem of heat and mass transfer free convection flow of Casson fluid over a porous nonlinear stretching sheet in the presence of chemical reaction is investigated. Moreover the effect of magnetic field is also considered. The governing partial differential equations are transformed into ordinary differential equations by making use of suitable transformations and then solved numerically via Keller-box method. The results for skin friction are compared with previous results of the existing literature. The results are also reflected in good agreement. It is noted that concentration of Casson fluid reduces rapidly by increasing Schmidt number and chemical parameter. Also, thermal Grashof number and mass Grashof number enhance the momentum boundary layer thickness, whereas increment in chemical reaction parameter reduces the heat transfer rate. Moreover, both the fluid velocity and wall shear stress are observed to be decreased with increment in suction/blowing parameter.

Reproductive Sciences, 2018

Porcine mesenchymal stem cells (MSCs) are similar to human MSCs, hence considered a valuable mode... more Porcine mesenchymal stem cells (MSCs) are similar to human MSCs, hence considered a valuable model for assessing potential for cell therapy. Porcine adipose-derived MSCs (AD-MSCs) and endometrial stromal MSCs (EMSCs) displayed fibroblast-like morphology and were positive for MSC markers CD73, CD90, and CD105 and negative for hematopoietic markers CD34 and CD45. The EMSCs had similar or slightly higher growth rate compared to AD-MSCs, and similar percentage of cells of both EMSCs and AD-MSCs were at G0/G1 and G2/M phases; however, EMSCs had significantly (P < .05) higher percentage of cells at S phase of cell cycle than AD-MSCs. Transdifferentiation ability to cardiomyocyte-like cells was confirmed in differentiated cells by the expression of lineage-specific marker genes such as DES, ACTA2, cTnT, and ACTC1 by real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, cardiomyocyte-specific protein markers cTnT and ACTC1 were expressed in completely differentiated cells. Endodermal differentiation capacity of EMSCs to pancreatic b cell-like cells was evident with the changes in morphology and the expression of b-cell-specific marker genes such as PDX1, GLUT2, SST, NKX6.1, PAX4, and NGN3 as analyzed by RT-qPCR. The differentiated cells secreted insulin and C-peptide upon glucose challenge and also they expressed insulin, PDX1, PAX4, NGN3, and GLUT2 at protein level as assessed by immunostaining confirming the successful differentiation to b cell-like cells. Porcine EMSCs possess all the characteristics of MSCs and are suitable model for studying molecular mechanisms of cellular differentiation.

Journal of Embryo Transfer, 2018

Journal of Embryo Transfer, 2017

Autophagy is an intracellular degradation and recycling system. Oocyte maturation is dynamic proc... more Autophagy is an intracellular degradation and recycling system. Oocyte maturation is dynamic process, in which various proteins should be synthesized and degraded. In our previous study, we reported the loci of autophagosome and dynamics of autophagic activity in porcine oocytes during in vitro maturation. In this study, we verified loci of autophagosome in porcine follicular cumulus-oocyte complex by detection of microtubule-associated protein 1A/1B-light chain 3 (LC3) which is the reliable marker of autophagosome. Porcine ovary including various sizes of follicles was fixed within 1 hour after collection from slaughterhouse. After fixation, immunohistochemistry was conducted on sliced ovary tissue containing various sizes of follicles by using LC3 antibody. As a result, LC3 signal was clearly detected in both cumulus and oocytes of various sizes of follicles. We also found ring shaped signal which represent autophagosome near oocyte membrane. Most of the signals in oocytes were localized nearby cellular membrane while evenly dispersed in cumulus cells. Therefore, this result suggests that autophagy occurs in porcine COCs (cumulus-oocyte complexes) at follicular stage.

Nanoscale research letters, 2016

In the present work, the effects of chemical reaction on hydromagnetic natural convection flow of... more In the present work, the effects of chemical reaction on hydromagnetic natural convection flow of Casson nanofluid induced due to nonlinearly stretching sheet immersed in a porous medium under the influence of thermal radiation and convective boundary condition are performed numerically. Moreover, the effects of velocity slip at stretching sheet wall are also examined in this study. The highly nonlinear-coupled governing equations are converted to nonlinear ordinary differential equations via similarity transformations. The transformed governing equations are then solved numerically using the Keller box method and graphical results for velocity, temperature, and nanoparticle concentration as well as wall shear stress, heat, and mass transfer rate are achieved through MATLAB software. Numerical results for the wall shear stress and heat transfer rate are presented in tabular form and compared with previously published work. Comparison reveals that the results are in good agreement. F...

International journal of medical sciences, 2016

The purpose of the present study was to investigate the in vitro cardiomyogenic differentiation p... more The purpose of the present study was to investigate the in vitro cardiomyogenic differentiation potential of human dental follicle-derived stem cells (DFCs) under the influence of suberoylanilide hydroxamic acid (SAHA), a member of the histone deacetylase inhibitor family, and analyze the in vivo homing capacity of induced cardiomyocytes (iCMs) when transplanted systemically. DFCs from extracted wisdom teeth showed mesenchymal stem cell (MSC) characteristics such as plate adherent growing, expression of MSC markers (CD44, CD90, and CD105), and mesenchymal lineage-specific differentiation potential. Adding SAHA to the culture medium induced the successful in vitro differentiation of DFCs into cardiomyocytes. These iCMs expressed cardiomyogenic markers, including alpha-smooth muscle actin (α-SMA), cardiac muscle troponin T (TNNT2), Desmin, and cardiac muscle alpha actin (ACTC1), at both the mRNA and protein level. For the assessment of homing capacity, PKH26 labeled iCMs were intraper...

Journal of Energy Technologies and Policy, 2015

Porcine mesenchymal stem cells (MSCs) are of great interest due to their potential to differentia... more Porcine mesenchymal stem cells (MSCs) are of great interest due to their potential to differentiate into multilineages as a good candidate for regenerative medicine (Rho et al., 2009). Porcine uteri were obtained from slaughter house (n=5) and endometrial stromal cells were isolated and plated 500 cells/cm3 in ADMEM containing 10% FBS to obtain purified single suspensions of stromal cells. Isolated stromal cells were analyzed for mesenchymal markers, pluripotent genes, cardiogenic differentiation, and finally homing of in vivo injected cells. The isolated cells were positive for mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b, and CD144, negative for epithelial marker CD9, hematopoietic markers CD34 and CD45 analyzed by flow cytometry and Semi-Quantitative PCR. Pluripotent markers OCT4, SOX2, and NANOG were positively expressed by western blotting and semi-Quantitative PCR, proving they were stem cell in origin rather than fibroblast (Li H Y et al., 2010). Further, the...

This investigation aimed to examine the intrinsic antimicrobial susceptibility and resistance of ... more This investigation aimed to examine the intrinsic antimicrobial susceptibility and resistance of Streptomyces species, isolated from the sediments of selected thermal springs located in northern Pakistan. Isolated species were subjected to morphological, physiological; biochemical and microscopic analyses. The isolates were examined for the consumption of various carbon sources, deprivation of complex compounds, susceptibility and resistance to antimicrobials and inhibitory compounds. The results indicated that all isolates demonstrated complete resistances to penicillin and ampicillin and weak resistance to Cotrimoxazole, Cephazolin, Deoxycline and Lincomycine. These isolates were moderately susceptible to Erythromycin, Cefaparazone, Cefapime, Amoxicillin and Cephradine while completely susceptible to Tetracycline, Vancomycin, Amikacin Gentamicin and Imipenem. In addition, Streptomyces isolates were more susceptible to Vancomycin and Gentamicin as compared to other antimicrobials a...

International Journal of Molecular Sciences, 2015

Endometrial stromal cells (EMSCs) obtained from porcine uterus (n = 6) were positive for mesenchy... more Endometrial stromal cells (EMSCs) obtained from porcine uterus (n = 6) were positive for mesenchymal stem cell markers (CD29, CD44 and CD90), and negative for epithelial marker CD9 and hematopoietic markers CD34, CD45 analyzed by flow cytometry. Further the cells were positive for expression of mesenchymal markers, CD105, CD140b, and CD144 by PCR. Pluripotent markers OCT4, SOX2, and NANOG were positively expressed in EMSCs analyzed by Western blotting and PCR. Further, differentiation into adipocytes and osteocytes was confirmed by cytochemical staining and lineage specific gene expression by quantitative realtime-PCR. Adipocyte (FABP, LPL, AP2) and osteocyte specific genes (ON, BG, RUNX2) in differentiated EMSCs showed significant (p < 0.05) increase in expression compared to undifferentiated control cells. Neurogenic

Bioscience Reports, 2015

Stem cells are cells specialized cell, capable of renewing themselves through cell division and c... more Stem cells are cells specialized cell, capable of renewing themselves through cell division and can differentiate into multi-lineage cells. These cells are categorized as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult stem cells. Mesenchymal stem cells (MSCs) are adult stem cells which can be isolated from human and animal sources. Human MSCs (hMSCs) are the non-haematopoietic, multipotent stem cells with the capacity to differentiate into mesodermal lineage such as osteocytes, adipocytes and chondrocytes as well ectodermal (neurocytes) and endodermal lineages (hepatocytes). MSCs express cell surface markers like cluster of differentiation (CD)29, CD44, CD73, CD90, CD105 and lack the expression of CD14, CD34, CD45 and HLA (human leucocyte antigen)-DR. hMSCs for the first time were reported in the bone marrow and till now they have been isolated from various tissues, including adipose tissue, amniotic fluid, endometrium, dental tissues, umbilical cord a...

Asian Pacific Journal of Tropical Biomedicine, 2014

under teaching and learning program (UoG/Budget code: 6417).

Journal of Solution Chemistry, 2014

The development of a meaningful dissolution procedure for drug products with limited water solubi... more The development of a meaningful dissolution procedure for drug products with limited water solubility has been a challenge to both the pharmaceutical industry and the agencies that regulate them. On the other hand, poorly soluble drugs present a big problem in pharmaceutical formulations. Therefore, various methods to improve the dissolution of poorly soluble drugs have been reported and employing surfactants for this purpose is the leading approach. In spite of wide applications of Mefenamic acid, the enhancement of its solubility in water using surfactant systems has not been explored. Therefore, we have investigated the interaction between this drug and anionic (SDS, SDBS, DTAB, CTAB, TTAB) surfactants as well as non-ionic (Tween 20, Tween 40, Tween 60, Tween 80, Brij 30, Brij 35,Brij 56 and Brij 58) surfactants. The results show that, irrespective of the surfactant type, the solubility of Mefenamic acid increases with increase in concentration of a surfactant, leading to the conclusion that there is an association between the drug and the surfactants. The molar solubilization ratio, micelle-water partition coefficient (K M), binding constant (K) and the Gibbs energy of solubilization (DG o s) of the drug in the micelles were determined to yield a model to correlate the solubility with the structure of the surfactant used. An outcome of the results is that the order of solubility of this drug in non-ionic and ionic surfactants is Tween 80 [ Tween 60 [ Tween 40 [ Tween 20 and, in the case of the Brij series, is Brij56 [ Brij58 [ Brij35 [ Brij30, while in cationic and anionic surfactants the order is CTAB [ TTAB [ DTAB and SDBS [ SDS.

Journal of Freshwater Ecology, 1994

ABSTRACT This study evaluated several habitat features relative to the natural distribution of Eu... more ABSTRACT This study evaluated several habitat features relative to the natural distribution of Eurasian watermilfoil (Myriophyllum spicatum L.) in Lake Wingra, Wisconsin, using data collected in 1970, when the biomass of this macrophyte was near its historic peak in the lake. Water depth, biomass of other species, and the extinction coefficient of light, in that order, were significant factors for predicting milfoil biomass. Because of multiple correlations between factors, these parameters were probably also acting as surrogate representatives for other factors. A linear regression model for predicting milfoil biomass was weak, but optimal ranges of water depth, substrate organic matter, and substrate texture for milfoil growth were identified.