Irada Huseynova - Academia.edu (original) (raw)
Papers by Irada Huseynova
Land plants shorten their vegetation period during water deficiency. Under water stress, wheat pl... more Land plants shorten their vegetation period during water deficiency. Under water stress, wheat plants undergo several
changes at the morphophysiological level. We tried to elucidate the role of the morphophysiological parameters and the
photosynthetic machinery in response to drought followed by rewatering. Therefore, some morphophysiological traits
of two bread wheat genotypes (drought-tolerant Gobustan, drought-sensitive Tale 38) were studied. The H2O2 content
increased under drought in both genotypes but recovered in the Gobustan genotype after rewatering. The isozymes of
peroxidase manifested dynamic changes under drought. The electron transport rate and the maximum photochemical
quantum efficiency of PSII showed similar responses to drought with subsequent rewatering in both genotypes. However,
the amount of the photosynthetic pigments changed drastically resulting in structural changes of thylakoid membranes.
In Gobustan, the thylakoid membrane structure almost completely recovered after rewatering. Thus, the drought-tolerant
genotype shows a more dynamic response of photosynthetic machinery and antioxidant capacity.
Photosynthesis Research, 2016
Water deficit is a key factor influencing the yield and quality of crops. In the present study, t... more Water deficit is a key factor influencing the
yield and quality of crops. In the present study, the photosynthetic
responses by means of chlorophyll fluorescence
of chloroplasts, thylakoid membrane proteins, and antioxidant
components were analyzed in wheat (Triticum durum
Desf.) plants differing in their tolerance to drought. Two
durum winter wheat varieties, Barakatli 95 (drought tolerant)
and Garagylchyg 2 (drought sensitive) were grown
under field well-watered and drought conditions. It was
found that contents of the PS I core (CPI) with Mr of 123
kD and apoprotein P700 with Mr of 63 kD were relatively
higher in Barakatli 95 variety under drought stress compared
with the control plants. Synthesis of a- and b-subunits
of CF1 ATP-synthase complex with Mr of 55 and
53.5 kD also slightly increased in the tolerant Barakatli 95
and decreased in the drought sensitive variety Garagylchyg
2. A decrease in the intensity of 30 kD band and a significant
increase were found in the content of the 25–16 kD
region in Garagylchyg 2 variety. The synthesis of 60 kD
and content of low molecular mass polypeptides (21.5 and
12 kD) were increased in the tolerant genotype Barakatli
95. The intensity of peaks at 687, 695, and 742 nm considerably
increases in the fluorescence spectra (77 K) of
chloroplasts isolated from the sensitive variety Garagylchyg
2, and there is a stimulation of the ratio of fluorescence
band intensity F687/F740. At the same time, higher level of glycine betaine was found in the drought
tolerant variety compared with the control one throughout
the different periods of growth
Photosynthesis Research, 2015
The dynamics of the activity of catalase, ascorbate peroxidase, guaiacol peroxidase, and benzidin... more The dynamics of the activity of catalase,
ascorbate peroxidase, guaiacol peroxidase, and benzidine
peroxidase, as well as the level of hydrogen peroxide in the
vegetative organs of durum wheat (Triticum durum Desf.)
cultivars was studied under long-term soil drought conditions.
It was established that hydrogen peroxide generation
occurred at early stages of stress in the tolerant variety
Barakatli-95, whereas in the susceptible variety Garagylchyg-
2 its significant amounts were accumulated only
at later stages. Garagylchyg-2 shows a larger reduction of
photochemical activity of PS II in both genotypes at all
stages of ontogenesis under drought stress than Barakatli-
95. The highest activity of catalase which plays a leading
role in the neutralization of hydrogen peroxide was observed
in the leaves and roots of the drought-tolerant variety
Barakatli-95. Despite the fact that the protection
system also includes peroxidases, the activity of these enzymes
even after synthesis of their new portions is substantially
lower compared with catalase. Native PAGE
electrophoresis revealed the presence of one isoform of
CAT, seven isoforms of APX, three isoforms of GPO, and
three isoforms of BPO in the leaves, and also three isoforms
of CAT, four isoforms of APX, two isoforms of
GPO, and six isoforms of BPO in the roots of wheat. One
isoform of CAT was found in the roots when water supply
was normal and three isoforms were observed under
drought conditions. Stress associated with long-term soil
drought in the roots of wheat has led to an increase in the
heterogeneity due to the formation of two new sedentary
forms of catalase: CAT2 and CAT3.
PHOTOSYNTHETICA, 2018
Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to eval... more Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to evaluate the incidence of main Tobamoviruses. According to results of serological and molecular tests, Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Pepper mild mottle virus (PMMoV) were detected as single and mixed infections (TMV + PMMoV; ToMV + PMMoV) in various tomato samples. It was found that Tobamovirus infection caused an increase in the content of malondialdehyde, alterations in the activities of peroxidase enzymes and quantitative and qualitative changes in their molecular isoforms. A comparison of thylakoid membrane polypeptides from virus-infected leaves indicated a decrease in the content of the thylakoid membrane polypeptides with molecular masses of 123, 55, 47, 33, 28-24, 17, and 15 kD. PSII efficiency and the content of chlorophylls (a and b) were significantly lower in the virus-infected leaves.
An International Journal of Applied Plant Pathology, 2016
During surveys conducted in September 2015, different red grapevine (Vitis vinifera L.) varieties... more During surveys conducted in September 2015, different red grapevine (Vitis vinifera L.) varieties with leaf reddening, drying of grapes, and leaf rolling symptoms reminiscent phytoplasma infections were observed in the main grape growing areas of Azerbaijan, such as Ganja, Samukh, Gabala regions and the Absheron peninsula. The disease incidence and severity ranged from 3 to 16% in different vineyards. Total nucleic acids were extracted from fresh leaf midribs and petioles using a CTAB method. Extracts were submitted to 16S-rDNA nested PCR with the universal primers for phytoplasmas R16mF2/R16mR1 and
Functional Plant Biology, 2020
A characteristic feature of C 4 plants is the differentiation of the photosynthetic leaf tissues ... more A characteristic feature of C 4 plants is the differentiation of the photosynthetic leaf tissues into two distinct cell types: mesophyll (M) and bundle sheath (BS) cells. We have investigated several biochemical parameters, including pigment composition, polypeptide patterns, fluorescence at 77K, the activity of photosystems and ultrastructure of mesophyll and bundle sheath chloroplasts of maize (Zea mays L.) plants. It is shown that the BS chloroplasts have~2-fold higher chlorophyll a/b ratio than M chloroplasts, 6.15 and 3.12 respectively. The PSI apoprotein (68 kDa) was more abundant in BS than in M thylakoids. Polypeptides belonging to PSII core antenna, are in similar amounts in both types of membranes, but the 45kDa band is more intensive in M thylakoids. Polypeptides in the region of 28-24 kDa of the light-harvesting complex of PSII (LHCII) are also present in both types of chloroplasts, though their amounts are reduced in BS thylakoids. The chlorophyll fluorescence emission spectra in M cells showed the presence of three bands at 686, 695 and 735 nm characteristics of LHCII, PSII core and PSI complexes, respectively. However, in the fluorescence spectrum of agranal plastids, there are almost traces of the band at 695 nm, which belongs to the PSII core complex. The research results revealed that the photochemical activity of PSII in BS chloroplasts is~5 times less than in the chloroplasts of M cells. The highest PSI activity was found in maize BS chloroplasts.
The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H 2 O 2 scavenging in plants. The... more The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H 2 O 2 scavenging in plants. The effect of diurnal variations in hydrogen peroxide (H 2 O 2) content, the intensity of lipid peroxidation (malondialdehyde, MDA), photosynthesis, antioxidants and antioxidative enzyme activities involved in AsA-GSH metabolism has been studied comparatively in leaves of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes exposed to soil drought. Drought stress caused an increase in the content of H 2 O 2 , MDA, alterations in the activities of AsA-GSH cycle enzymes and quantitative changes in AsA and GSH content during the day. PSII efficiency was significantly lower in the control and drought exposed leaves at the highest temperature in the afternoon. The ascorbate peroxidase activity was found to increase and ascorbic acid amount decreased with increasing temperature during the day. Further, the glutathione amount and glutathione reductase activity increased at the expense of the regeneration of the oxidised form of glutathione. Our results revealed that wheat can tolerate drought stress by enhancing the antioxidant enzyme activities and alteration of the concentration of ascorbate and glutathione.
The objective of work was to identify effective yellow and brown leaf rust resistance genes for t... more The objective of work was to identify effective yellow and brown leaf rust resistance genes for the improvement of wheat quality in Azerbaijan. 61 bread wheat (Triticum aestivum L.) genotipes were taken from Research Institute of Crop Husbandry (Baku, Azerbaijan). PCR amplification of the SSR marker located on the chromosome 7D Xgwm295 linked to the Yr18 gene showed a fragment of 250 bp only in 40 genotypes. SSR marker XGWM582 in the chromosome 1B, associated with the gene Yr9 amplified a band of 150 bp in 93% of genotypes. The STS markers Lr34/csLV34a and Lr34/csLV34b were proved as effective markers for the identification and selection of Lr 34 gene. The csLV34a allele (229-bp) was linked in repulsion with Lr34 presented in 21 genotypes, whereas the csLV34b allele (150-bp) was linked in coupling with Lr34 in only 9 genotypes. 2 genotypes contain both alleles linked to the Lr34. ABSTRACT KEYWORDS : yellow rust, Yr 18, Yr9, brown leaf rust, Lr 34, Triticum aestivum L.
Springer Nature Singapore, 2019
Global climate change, which is rising steeply in the world today, has caused further increases i... more Global climate change, which is rising steeply in the world today, has
caused further increases in the influence of abiotic stress factors. Abiotic stresses such as drought, high salinity, and high temperatures are common detrimental environmental circumstances that extremely influence growth, cultivation, and productivity worldwide. Traditionally, the varietal selection is based on morphological feature; hence, polygenic characters were very difficult to analyze, and thus such
constraints can be overcome by using molecular marker-assisted selection (MAS). As markers are currently available for relatively few traits, MAS must be integrated with the ongoing conventional breeding to maximize its impact. Molecular markers are useful tools to determine the variation in the DNA sequence and eventually the
regions of DNA or the genes controlling the qualitative and/or quantitative traits of agronomic significance. The aim of the presented research was to detect the genes and gene loci responsible for the tolerance to salt stress, drought, and high temperatures in wheat using various molecular markers. Wheat genotypes with contrasting stress tolerance, architectonics, productivity, and other physiological traits kept in the gene fund of the Research Institute of Crop Husbandry were used
as research objects. Plants were cultivated in the Absheron experimental base under natural conditions (Baku, Azerbaijan). Salt tolerance potential of wheat genotypes was examined genetically based on PCR analysis using various molecular markers associated with salt tolerance. According to the results of the molecular analysis performed with RAPD (OPZ 09) markers linked with salt tolerance, expected 590 bp DNA fragments were amplified in 39% of bread wheat and 27% of durum wheat genotypes. At the same time, salt tolerance gene Nax1 was examined with gwm312 primer in durum wheat genotypes. An expected 200 bp amplicon indicating the existence of this gene in the Barakatli 95, Garabag, Shiraslan 23, Sharg, and Gyrmyzy bugda genotypes was successfully amplified. This amplicon was also synthesized in Barakatli 95, Garabag, Shiraslan 23, and Gyrmyzy bugda genotypes using wmc170 primer linked to salt tolerance locus in durum wheat genotypes. The study of physiological senescence of the flag leaf playing the pivotal role in the uptake of solar energy and stipulating plant productivity in wheat is very important for providing high productivity under stress. Therefore, using RAPD OPH13 marker, the existence of a gene locus linked to the physiological life-span of flag leaf was examined in wheat genotypes under drought stress. Based on the analysis of electrophoretic profiles of PCR results, expected 450 bp fragments were synthesized in 30 wheat genotypes. This result confirms the existence of a gene locus providing the physiological youth of flag leaf, which is considered as the drought tolerance indicator in these genotypes. The existence of the studied locus was not confirmed in 19% of the genotypes. Tolerance to heat stress is a complex phenomenon and controlled by multiple genes imparting a number of physiological and biochemical changes. To differentiate the heat-tolerant and heat-susceptible genotypes of wheat, a heat shock protein (HSP16.9) was taken as a target gene, and domestic genotypes were examined using allele-specific PCR primers. The obtained results can be used in wheat breeding programs for developing wheat varieties tolerant to stresses.
Virology Journal, 2020
Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through nat... more Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through natural spread of
Cryphonectria hypovirus 1 (CHV1), a mycovirus able to induce hypovirulence to the host. In recent years
C. parasitica was reported infecting Azerbaijani population of chestnut, but the presence of CHV1 still needs to be
confirmed. Aim of this work was to investigate fifty-five C. parasitica isolates collected in Azerbaijan to describe
the associated viruses. Our work found i) the first negative-sense ssRNA virus known to infect C. parasitica
naturally for which we propose the name Cryphonectria parasitica sclerotimonavirus 1 (CpSV1) and ii) an RNA
sequence showing peculiar features suggesting a viral nature for which we propose the name Cryphonectria
parasitica ambivirus 1 (CpaV1). The discovery of CpaV1 expands our knowledge of the RNA virosphere suggesting
the existence of a new lineage that cannot presently be reliably associated to the monophyletic Riboviria.
Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, 2017
Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing diffe... more Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing different symptoms that ranges from yellowing to death of infected plants. The aim of the present work was to analyze the changes in the activity of metabolic and antioxidant enzymes, as well as in the content of total soluble sugars, tocopherols, and total phenolic compounds in field-grown pepper plants (Capsicum annuum L.) under the influence of phytoplasma infection. The activities of benzidine peroxidase (BPO) and guaiacol peroxidase (GPO) were observed to increase in infected pepper leaves in comparison with the healthy control. According to the native polyacrylamide gel electrophoresis (PAGE), the levels of studied antioxidant enzyme isoforms were enhanced under pathogenesis. Activities of aspartate aminotransferase (AsAT) and alanine aminotransferase (ALAT) were also increased in the infected plants compared with healthy plants. The amount of tocopherols, soluble sugars, and total phenols was significantly higher in leaves due to the phytoplasma infection. In conclusion, all of the observed alterations could be considered to be the response and the adaptation ability of pepper plants against pathogen infection.
Journal of Photochemistry &Photobiology, C: Photochemistry Reviews, 2018
Over the last two decades many reports have been published on diverse types of biohybrid electrod... more Over the last two decades many reports have been published on diverse types of biohybrid electrodes utilizing components of the photosynthetic apparatus. Currently, the development of such devices does not extend beyond laboratory research. In the future, these electrodes could be used in biosensors, solar cells, and as a new technique to investigate photosynthetic pigment-protein complexes. Efficiency of light-to-current conversion is particularly important for solar cell applications. Selection of a suitable substrate for special pigment-protein complexes is a significant challenge for building an inexpensive and efficient device. Various combinations of pigment-protein complexes and substrates, as well as different measurement conditions make it difficult to directly compare performance of various solar cells. However, it has been shown, that one of the possible substrate materials, namely nanostructured TiO 2 , is the most preferred material for the immobilization of pigment-protein complexes in terms of both cost and efficiency. The photocurrent values reaching several mA, were reported for TiO 2-based biohybrid electrodes. However, the efficiency of TiO 2-based biohybrid is still far from its potential maximum value due to fundamental challenges related to designing an optimum interface between TiO 2 nanostructure and pigment-protein complexes containing electron transferring cofactors. To date, counterproductive back reactions, also referred to as charge recombina-tion, still dominate and lower internal quantum efficiency of these systems.
Net Journals of Agricultural Sciences, 2017
Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing diffe... more Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing different symptoms that ranges from yellowing to death of infected plants. The aim of the present work was to analyze the changes in the activity of metabolic and antioxidant enzymes, as well as in the content of total soluble sugars, tocopherols, and total phenolic compounds in field-grown pepper plants (Capsicum annuum L.) under the influence of phytoplasma infection. The activities of benzidine peroxidase (BPO) and guaiacol peroxidase (GPO) were observed to increase in infected pepper leaves in comparison with the healthy control. According to the native polyacrylamide gel electrophoresis (PAGE), the levels of studied antioxidant enzyme isoforms were enhanced under pathogenesis. Activities of aspartate aminotransferase (AsAT) and alanine aminotransferase (ALAT) were also increased in the infected plants compared with healthy plants. The amount of tocopherols, soluble sugars, and total phenols was significantly higher in leaves due to the phytoplasma infection. In conclusion, all of the observed alterations could be considered to be the response and the adaptation ability of pepper plants against pathogen infection.
Physiological and Molecular Plant Pathology, 2019
Transactions of the Institute of Molecular Biology & Biotechnologies, 2017
The study of plant adaptation to drought stress as well as the assessment of plant restoration ab... more The study of plant adaptation to drought stress as well as the assessment of plant restoration ability after water deficiency is one of the actual problems of the modern science. Gobustan and Tale 38 bread wheat genotypes exposed to long-term soil drought were rewatered. Leaf samples were taken after 3 and 7-day rehydration and dynamics of H 2 O 2 accumulation was studied histochemically, relative water content (RWC), amounts of hydrogen peroxide (H 2 O 2), ascorbic acid (AsA) and reduced glutathione (GSH) were measured and the recovery process was assessed in watered and drought variants, comparatively. Water loss in leaves of the tolerant genotype was found to be less compared with the sensitive genotype. The amount of reduced glutathione increased in both genotypes under drought and after rehydration this amount decreased in the tolerant genotype, while staying at the high level in the sensitive genotype. The amount of ascorbic acid did not change significantly in the Gobustan variety, whereas it changed substantially in the Tale-38 variety. The obtained data confirm that antioxidant defense system functions quiet efficiently under drought. These genotypes differently responding to water deficiency protect their ability of partly restoration after rehydration.
Transactions of the Institute of Molecular Biology & Biotechnologies, 2017
TRAP9 marker linked to the QTL for cell membrane stability was used for the assessment of drought... more TRAP9 marker linked to the QTL for cell membrane stability was used for the assessment of drought tolerance in wheat genotypes. 290 bp fragments diagnostic for this marker were synthesized in the all studied 17 durum wheat (Triticum durum Desf.) genotypes. Positive results were obtained for 92% of the studied 52 bread wheat (Triticum aestivum L.) genotypes. It is suggested that QTL for cell membrane stability is present on the 5th chromosome of the genome A of these genotypes. At the same time only four bread wheat lines are exceptions.
Proceedings of ANAS (Biological and Medical Sciences), 2017
A method of using agaroponics for studying the resistance and adaptability of wheat plants to str... more A method of using agaroponics for studying the resistance and adaptability of wheat plants to stress effects is proposed. The possibility of modeling the increasing salt stress effect is shown.
Drought is a major limiting factor affecting wheat production in the world. We aimed to study the... more Drought is a major limiting factor affecting wheat production in the world. We aimed to study the effect of soil water deficit on dry matter remobilization (DMR), grain yield (GY) and yield components of durum and bread wheat genotypes. Drought stress accelerated DMR. Lowest remobilization of dry matter into grains was detected in the tallest, late heading genotypes, which were also characterized by low harvest index (HI). Drought stress showed less affect on plant height (PH), peduncle length (PL), spike length (SL), spike width (SW), spikelet number per spike (SNS) but strongly affected the biological yield (BY), spike mass (SM), grain number per spike (GNS) and grain mass per spike (GMS), thousand kernels mass (TKM). GY positively and significantly correlated with spikes m-2 (SN), BY and HI under drought stress condition. We consider that wheat characteristics DMR, SN, BY, HI are good selection criteria under drought stress.
European Journal of Plant Pathology, 2018
Bois noir (BN) is an important grapevine yellows endemic to the Euro-Mediterranean basin caused b... more Bois noir (BN) is an important grapevine yellows
endemic to the Euro-Mediterranean basin caused by
‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), a non
culturable plant pathogenic Mollicute. Bois noir symptoms
could be associated with ‘Ca. P. solani’ in two
Azerbaijanian vineyards where disease incidence and severity
were recorded for five local Vitis vinifera cultivars.
In order to gain insight into the epidemiology of Bois noir
in Azerbaijan, ‘Ca. P. solani’ isolates infecting plants were
characterized by multi-locus sequence analysis and their
secY and stamp gene sequences compared to that of the
strains detected in other plants and in local Cixiidae
planthoppers. Genotypes were determined for two nonribosomal
house-keeping genes, namely tuf and secY, as
well as two variable markers namely Stamp and mleP1
genes, that respectively encode the antigenic membrane
protein AMP and a 2-Hydroxycarboxylate transporter. The
Azerbaijanian BN phytoplasma isolates corresponded to
three tufB and secY genotypes. A finer differentiation of
Azerbaijanian ‘Ca. P. solani’ isolates was obtained with
mleP1 as five different mleP1 genetic variants were found.
Finally, Stamp gene allowed differentiating four new genotypes
in grapevine among the 10 new Stamp genotypes
detected in various plants in Azerbaijan. The preliminary
survey for infected insects conducted in northern Azerbaijan,
led to the identification of Hyalesthes obsoletus and
Reptalus noahi as potential vectors for two ‘Ca. P. solani’
new genotypes phylogenetically distant from the known
genetic clusters. Altogether these results indicate an important
genetic diversity of BN phytoplasmas in Azerbaijan
that certainly result from spread through local insect
vectors.
Plant Physiology and Biochemistry Journal, 2014
Water is a key factor influencing the yield and quality of crops. One of the parameters of plant ... more Water is a key factor influencing the yield and quality of crops. One of the parameters of plant biological
tolerance to constantly changing environmental conditions is the change of activities and numerous molecular
forms of antioxidant enzymes. Two durum (Triticum durum Desf.) wheat varieties contrasting for
drought tolerance, such as Barakatli-95 (drought tolerant) and Garagylchyg-2 (drought sensitive) were
grown over a wide area in the field. Experiments were carried out to study the effect of soil drought on
changes in activities and subcellular localization of superoxide dismutase isoforms. The levels of malondialdehyde,
glycine betaine and total proteins were also analyzed. The level of the enzyme activity appeared
to depend on thewheat varieties, duration of drought and stages of leaf development.Native polyacrylamide
gel electrophoresis (PAGE) revealed the presence of 9 isoenzymes of superoxide dismutase in wheat leaves
during drought.Mn-SODwas foundin the mitochondrial fractions, Fe-SODinthe chloroplast fraction andCu/
Zn-SODis localized in all subcellular fractions.Wheat leaves contain three different isoforms ofSOD(Mn-, Fe-
, Cu/Zn-SOD). Three isoforms of Mn-SOD, one isoform of Fe-SOD and five of Cu/Zn-SOD were observed in
wheat leaves using 3 mM KCN and 5 mM H2O2 as selective inhibitors. The expression of Mn-SOD was
preferentially enhanced by drought stress. It seems that Mn-SOD isoforms more than SOD ones play a major
role in the scavenging of superoxide radicals. The observed data showed that status of antioxidant enzymes
such as SOD could provide a meaningful tool for depicting drought tolerance of wheat genotype.
Land plants shorten their vegetation period during water deficiency. Under water stress, wheat pl... more Land plants shorten their vegetation period during water deficiency. Under water stress, wheat plants undergo several
changes at the morphophysiological level. We tried to elucidate the role of the morphophysiological parameters and the
photosynthetic machinery in response to drought followed by rewatering. Therefore, some morphophysiological traits
of two bread wheat genotypes (drought-tolerant Gobustan, drought-sensitive Tale 38) were studied. The H2O2 content
increased under drought in both genotypes but recovered in the Gobustan genotype after rewatering. The isozymes of
peroxidase manifested dynamic changes under drought. The electron transport rate and the maximum photochemical
quantum efficiency of PSII showed similar responses to drought with subsequent rewatering in both genotypes. However,
the amount of the photosynthetic pigments changed drastically resulting in structural changes of thylakoid membranes.
In Gobustan, the thylakoid membrane structure almost completely recovered after rewatering. Thus, the drought-tolerant
genotype shows a more dynamic response of photosynthetic machinery and antioxidant capacity.
Photosynthesis Research, 2016
Water deficit is a key factor influencing the yield and quality of crops. In the present study, t... more Water deficit is a key factor influencing the
yield and quality of crops. In the present study, the photosynthetic
responses by means of chlorophyll fluorescence
of chloroplasts, thylakoid membrane proteins, and antioxidant
components were analyzed in wheat (Triticum durum
Desf.) plants differing in their tolerance to drought. Two
durum winter wheat varieties, Barakatli 95 (drought tolerant)
and Garagylchyg 2 (drought sensitive) were grown
under field well-watered and drought conditions. It was
found that contents of the PS I core (CPI) with Mr of 123
kD and apoprotein P700 with Mr of 63 kD were relatively
higher in Barakatli 95 variety under drought stress compared
with the control plants. Synthesis of a- and b-subunits
of CF1 ATP-synthase complex with Mr of 55 and
53.5 kD also slightly increased in the tolerant Barakatli 95
and decreased in the drought sensitive variety Garagylchyg
2. A decrease in the intensity of 30 kD band and a significant
increase were found in the content of the 25–16 kD
region in Garagylchyg 2 variety. The synthesis of 60 kD
and content of low molecular mass polypeptides (21.5 and
12 kD) were increased in the tolerant genotype Barakatli
95. The intensity of peaks at 687, 695, and 742 nm considerably
increases in the fluorescence spectra (77 K) of
chloroplasts isolated from the sensitive variety Garagylchyg
2, and there is a stimulation of the ratio of fluorescence
band intensity F687/F740. At the same time, higher level of glycine betaine was found in the drought
tolerant variety compared with the control one throughout
the different periods of growth
Photosynthesis Research, 2015
The dynamics of the activity of catalase, ascorbate peroxidase, guaiacol peroxidase, and benzidin... more The dynamics of the activity of catalase,
ascorbate peroxidase, guaiacol peroxidase, and benzidine
peroxidase, as well as the level of hydrogen peroxide in the
vegetative organs of durum wheat (Triticum durum Desf.)
cultivars was studied under long-term soil drought conditions.
It was established that hydrogen peroxide generation
occurred at early stages of stress in the tolerant variety
Barakatli-95, whereas in the susceptible variety Garagylchyg-
2 its significant amounts were accumulated only
at later stages. Garagylchyg-2 shows a larger reduction of
photochemical activity of PS II in both genotypes at all
stages of ontogenesis under drought stress than Barakatli-
95. The highest activity of catalase which plays a leading
role in the neutralization of hydrogen peroxide was observed
in the leaves and roots of the drought-tolerant variety
Barakatli-95. Despite the fact that the protection
system also includes peroxidases, the activity of these enzymes
even after synthesis of their new portions is substantially
lower compared with catalase. Native PAGE
electrophoresis revealed the presence of one isoform of
CAT, seven isoforms of APX, three isoforms of GPO, and
three isoforms of BPO in the leaves, and also three isoforms
of CAT, four isoforms of APX, two isoforms of
GPO, and six isoforms of BPO in the roots of wheat. One
isoform of CAT was found in the roots when water supply
was normal and three isoforms were observed under
drought conditions. Stress associated with long-term soil
drought in the roots of wheat has led to an increase in the
heterogeneity due to the formation of two new sedentary
forms of catalase: CAT2 and CAT3.
PHOTOSYNTHETICA, 2018
Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to eval... more Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to evaluate the incidence of main Tobamoviruses. According to results of serological and molecular tests, Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Pepper mild mottle virus (PMMoV) were detected as single and mixed infections (TMV + PMMoV; ToMV + PMMoV) in various tomato samples. It was found that Tobamovirus infection caused an increase in the content of malondialdehyde, alterations in the activities of peroxidase enzymes and quantitative and qualitative changes in their molecular isoforms. A comparison of thylakoid membrane polypeptides from virus-infected leaves indicated a decrease in the content of the thylakoid membrane polypeptides with molecular masses of 123, 55, 47, 33, 28-24, 17, and 15 kD. PSII efficiency and the content of chlorophylls (a and b) were significantly lower in the virus-infected leaves.
An International Journal of Applied Plant Pathology, 2016
During surveys conducted in September 2015, different red grapevine (Vitis vinifera L.) varieties... more During surveys conducted in September 2015, different red grapevine (Vitis vinifera L.) varieties with leaf reddening, drying of grapes, and leaf rolling symptoms reminiscent phytoplasma infections were observed in the main grape growing areas of Azerbaijan, such as Ganja, Samukh, Gabala regions and the Absheron peninsula. The disease incidence and severity ranged from 3 to 16% in different vineyards. Total nucleic acids were extracted from fresh leaf midribs and petioles using a CTAB method. Extracts were submitted to 16S-rDNA nested PCR with the universal primers for phytoplasmas R16mF2/R16mR1 and
Functional Plant Biology, 2020
A characteristic feature of C 4 plants is the differentiation of the photosynthetic leaf tissues ... more A characteristic feature of C 4 plants is the differentiation of the photosynthetic leaf tissues into two distinct cell types: mesophyll (M) and bundle sheath (BS) cells. We have investigated several biochemical parameters, including pigment composition, polypeptide patterns, fluorescence at 77K, the activity of photosystems and ultrastructure of mesophyll and bundle sheath chloroplasts of maize (Zea mays L.) plants. It is shown that the BS chloroplasts have~2-fold higher chlorophyll a/b ratio than M chloroplasts, 6.15 and 3.12 respectively. The PSI apoprotein (68 kDa) was more abundant in BS than in M thylakoids. Polypeptides belonging to PSII core antenna, are in similar amounts in both types of membranes, but the 45kDa band is more intensive in M thylakoids. Polypeptides in the region of 28-24 kDa of the light-harvesting complex of PSII (LHCII) are also present in both types of chloroplasts, though their amounts are reduced in BS thylakoids. The chlorophyll fluorescence emission spectra in M cells showed the presence of three bands at 686, 695 and 735 nm characteristics of LHCII, PSII core and PSI complexes, respectively. However, in the fluorescence spectrum of agranal plastids, there are almost traces of the band at 695 nm, which belongs to the PSII core complex. The research results revealed that the photochemical activity of PSII in BS chloroplasts is~5 times less than in the chloroplasts of M cells. The highest PSI activity was found in maize BS chloroplasts.
The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H 2 O 2 scavenging in plants. The... more The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H 2 O 2 scavenging in plants. The effect of diurnal variations in hydrogen peroxide (H 2 O 2) content, the intensity of lipid peroxidation (malondialdehyde, MDA), photosynthesis, antioxidants and antioxidative enzyme activities involved in AsA-GSH metabolism has been studied comparatively in leaves of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes exposed to soil drought. Drought stress caused an increase in the content of H 2 O 2 , MDA, alterations in the activities of AsA-GSH cycle enzymes and quantitative changes in AsA and GSH content during the day. PSII efficiency was significantly lower in the control and drought exposed leaves at the highest temperature in the afternoon. The ascorbate peroxidase activity was found to increase and ascorbic acid amount decreased with increasing temperature during the day. Further, the glutathione amount and glutathione reductase activity increased at the expense of the regeneration of the oxidised form of glutathione. Our results revealed that wheat can tolerate drought stress by enhancing the antioxidant enzyme activities and alteration of the concentration of ascorbate and glutathione.
The objective of work was to identify effective yellow and brown leaf rust resistance genes for t... more The objective of work was to identify effective yellow and brown leaf rust resistance genes for the improvement of wheat quality in Azerbaijan. 61 bread wheat (Triticum aestivum L.) genotipes were taken from Research Institute of Crop Husbandry (Baku, Azerbaijan). PCR amplification of the SSR marker located on the chromosome 7D Xgwm295 linked to the Yr18 gene showed a fragment of 250 bp only in 40 genotypes. SSR marker XGWM582 in the chromosome 1B, associated with the gene Yr9 amplified a band of 150 bp in 93% of genotypes. The STS markers Lr34/csLV34a and Lr34/csLV34b were proved as effective markers for the identification and selection of Lr 34 gene. The csLV34a allele (229-bp) was linked in repulsion with Lr34 presented in 21 genotypes, whereas the csLV34b allele (150-bp) was linked in coupling with Lr34 in only 9 genotypes. 2 genotypes contain both alleles linked to the Lr34. ABSTRACT KEYWORDS : yellow rust, Yr 18, Yr9, brown leaf rust, Lr 34, Triticum aestivum L.
Springer Nature Singapore, 2019
Global climate change, which is rising steeply in the world today, has caused further increases i... more Global climate change, which is rising steeply in the world today, has
caused further increases in the influence of abiotic stress factors. Abiotic stresses such as drought, high salinity, and high temperatures are common detrimental environmental circumstances that extremely influence growth, cultivation, and productivity worldwide. Traditionally, the varietal selection is based on morphological feature; hence, polygenic characters were very difficult to analyze, and thus such
constraints can be overcome by using molecular marker-assisted selection (MAS). As markers are currently available for relatively few traits, MAS must be integrated with the ongoing conventional breeding to maximize its impact. Molecular markers are useful tools to determine the variation in the DNA sequence and eventually the
regions of DNA or the genes controlling the qualitative and/or quantitative traits of agronomic significance. The aim of the presented research was to detect the genes and gene loci responsible for the tolerance to salt stress, drought, and high temperatures in wheat using various molecular markers. Wheat genotypes with contrasting stress tolerance, architectonics, productivity, and other physiological traits kept in the gene fund of the Research Institute of Crop Husbandry were used
as research objects. Plants were cultivated in the Absheron experimental base under natural conditions (Baku, Azerbaijan). Salt tolerance potential of wheat genotypes was examined genetically based on PCR analysis using various molecular markers associated with salt tolerance. According to the results of the molecular analysis performed with RAPD (OPZ 09) markers linked with salt tolerance, expected 590 bp DNA fragments were amplified in 39% of bread wheat and 27% of durum wheat genotypes. At the same time, salt tolerance gene Nax1 was examined with gwm312 primer in durum wheat genotypes. An expected 200 bp amplicon indicating the existence of this gene in the Barakatli 95, Garabag, Shiraslan 23, Sharg, and Gyrmyzy bugda genotypes was successfully amplified. This amplicon was also synthesized in Barakatli 95, Garabag, Shiraslan 23, and Gyrmyzy bugda genotypes using wmc170 primer linked to salt tolerance locus in durum wheat genotypes. The study of physiological senescence of the flag leaf playing the pivotal role in the uptake of solar energy and stipulating plant productivity in wheat is very important for providing high productivity under stress. Therefore, using RAPD OPH13 marker, the existence of a gene locus linked to the physiological life-span of flag leaf was examined in wheat genotypes under drought stress. Based on the analysis of electrophoretic profiles of PCR results, expected 450 bp fragments were synthesized in 30 wheat genotypes. This result confirms the existence of a gene locus providing the physiological youth of flag leaf, which is considered as the drought tolerance indicator in these genotypes. The existence of the studied locus was not confirmed in 19% of the genotypes. Tolerance to heat stress is a complex phenomenon and controlled by multiple genes imparting a number of physiological and biochemical changes. To differentiate the heat-tolerant and heat-susceptible genotypes of wheat, a heat shock protein (HSP16.9) was taken as a target gene, and domestic genotypes were examined using allele-specific PCR primers. The obtained results can be used in wheat breeding programs for developing wheat varieties tolerant to stresses.
Virology Journal, 2020
Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through nat... more Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through natural spread of
Cryphonectria hypovirus 1 (CHV1), a mycovirus able to induce hypovirulence to the host. In recent years
C. parasitica was reported infecting Azerbaijani population of chestnut, but the presence of CHV1 still needs to be
confirmed. Aim of this work was to investigate fifty-five C. parasitica isolates collected in Azerbaijan to describe
the associated viruses. Our work found i) the first negative-sense ssRNA virus known to infect C. parasitica
naturally for which we propose the name Cryphonectria parasitica sclerotimonavirus 1 (CpSV1) and ii) an RNA
sequence showing peculiar features suggesting a viral nature for which we propose the name Cryphonectria
parasitica ambivirus 1 (CpaV1). The discovery of CpaV1 expands our knowledge of the RNA virosphere suggesting
the existence of a new lineage that cannot presently be reliably associated to the monophyletic Riboviria.
Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, 2017
Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing diffe... more Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing different symptoms that ranges from yellowing to death of infected plants. The aim of the present work was to analyze the changes in the activity of metabolic and antioxidant enzymes, as well as in the content of total soluble sugars, tocopherols, and total phenolic compounds in field-grown pepper plants (Capsicum annuum L.) under the influence of phytoplasma infection. The activities of benzidine peroxidase (BPO) and guaiacol peroxidase (GPO) were observed to increase in infected pepper leaves in comparison with the healthy control. According to the native polyacrylamide gel electrophoresis (PAGE), the levels of studied antioxidant enzyme isoforms were enhanced under pathogenesis. Activities of aspartate aminotransferase (AsAT) and alanine aminotransferase (ALAT) were also increased in the infected plants compared with healthy plants. The amount of tocopherols, soluble sugars, and total phenols was significantly higher in leaves due to the phytoplasma infection. In conclusion, all of the observed alterations could be considered to be the response and the adaptation ability of pepper plants against pathogen infection.
Journal of Photochemistry &Photobiology, C: Photochemistry Reviews, 2018
Over the last two decades many reports have been published on diverse types of biohybrid electrod... more Over the last two decades many reports have been published on diverse types of biohybrid electrodes utilizing components of the photosynthetic apparatus. Currently, the development of such devices does not extend beyond laboratory research. In the future, these electrodes could be used in biosensors, solar cells, and as a new technique to investigate photosynthetic pigment-protein complexes. Efficiency of light-to-current conversion is particularly important for solar cell applications. Selection of a suitable substrate for special pigment-protein complexes is a significant challenge for building an inexpensive and efficient device. Various combinations of pigment-protein complexes and substrates, as well as different measurement conditions make it difficult to directly compare performance of various solar cells. However, it has been shown, that one of the possible substrate materials, namely nanostructured TiO 2 , is the most preferred material for the immobilization of pigment-protein complexes in terms of both cost and efficiency. The photocurrent values reaching several mA, were reported for TiO 2-based biohybrid electrodes. However, the efficiency of TiO 2-based biohybrid is still far from its potential maximum value due to fundamental challenges related to designing an optimum interface between TiO 2 nanostructure and pigment-protein complexes containing electron transferring cofactors. To date, counterproductive back reactions, also referred to as charge recombina-tion, still dominate and lower internal quantum efficiency of these systems.
Net Journals of Agricultural Sciences, 2017
Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing diffe... more Phytoplasmas are pathogens of important annual crops as well as perennial cultures, causing different symptoms that ranges from yellowing to death of infected plants. The aim of the present work was to analyze the changes in the activity of metabolic and antioxidant enzymes, as well as in the content of total soluble sugars, tocopherols, and total phenolic compounds in field-grown pepper plants (Capsicum annuum L.) under the influence of phytoplasma infection. The activities of benzidine peroxidase (BPO) and guaiacol peroxidase (GPO) were observed to increase in infected pepper leaves in comparison with the healthy control. According to the native polyacrylamide gel electrophoresis (PAGE), the levels of studied antioxidant enzyme isoforms were enhanced under pathogenesis. Activities of aspartate aminotransferase (AsAT) and alanine aminotransferase (ALAT) were also increased in the infected plants compared with healthy plants. The amount of tocopherols, soluble sugars, and total phenols was significantly higher in leaves due to the phytoplasma infection. In conclusion, all of the observed alterations could be considered to be the response and the adaptation ability of pepper plants against pathogen infection.
Physiological and Molecular Plant Pathology, 2019
Transactions of the Institute of Molecular Biology & Biotechnologies, 2017
The study of plant adaptation to drought stress as well as the assessment of plant restoration ab... more The study of plant adaptation to drought stress as well as the assessment of plant restoration ability after water deficiency is one of the actual problems of the modern science. Gobustan and Tale 38 bread wheat genotypes exposed to long-term soil drought were rewatered. Leaf samples were taken after 3 and 7-day rehydration and dynamics of H 2 O 2 accumulation was studied histochemically, relative water content (RWC), amounts of hydrogen peroxide (H 2 O 2), ascorbic acid (AsA) and reduced glutathione (GSH) were measured and the recovery process was assessed in watered and drought variants, comparatively. Water loss in leaves of the tolerant genotype was found to be less compared with the sensitive genotype. The amount of reduced glutathione increased in both genotypes under drought and after rehydration this amount decreased in the tolerant genotype, while staying at the high level in the sensitive genotype. The amount of ascorbic acid did not change significantly in the Gobustan variety, whereas it changed substantially in the Tale-38 variety. The obtained data confirm that antioxidant defense system functions quiet efficiently under drought. These genotypes differently responding to water deficiency protect their ability of partly restoration after rehydration.
Transactions of the Institute of Molecular Biology & Biotechnologies, 2017
TRAP9 marker linked to the QTL for cell membrane stability was used for the assessment of drought... more TRAP9 marker linked to the QTL for cell membrane stability was used for the assessment of drought tolerance in wheat genotypes. 290 bp fragments diagnostic for this marker were synthesized in the all studied 17 durum wheat (Triticum durum Desf.) genotypes. Positive results were obtained for 92% of the studied 52 bread wheat (Triticum aestivum L.) genotypes. It is suggested that QTL for cell membrane stability is present on the 5th chromosome of the genome A of these genotypes. At the same time only four bread wheat lines are exceptions.
Proceedings of ANAS (Biological and Medical Sciences), 2017
A method of using agaroponics for studying the resistance and adaptability of wheat plants to str... more A method of using agaroponics for studying the resistance and adaptability of wheat plants to stress effects is proposed. The possibility of modeling the increasing salt stress effect is shown.
Drought is a major limiting factor affecting wheat production in the world. We aimed to study the... more Drought is a major limiting factor affecting wheat production in the world. We aimed to study the effect of soil water deficit on dry matter remobilization (DMR), grain yield (GY) and yield components of durum and bread wheat genotypes. Drought stress accelerated DMR. Lowest remobilization of dry matter into grains was detected in the tallest, late heading genotypes, which were also characterized by low harvest index (HI). Drought stress showed less affect on plant height (PH), peduncle length (PL), spike length (SL), spike width (SW), spikelet number per spike (SNS) but strongly affected the biological yield (BY), spike mass (SM), grain number per spike (GNS) and grain mass per spike (GMS), thousand kernels mass (TKM). GY positively and significantly correlated with spikes m-2 (SN), BY and HI under drought stress condition. We consider that wheat characteristics DMR, SN, BY, HI are good selection criteria under drought stress.
European Journal of Plant Pathology, 2018
Bois noir (BN) is an important grapevine yellows endemic to the Euro-Mediterranean basin caused b... more Bois noir (BN) is an important grapevine yellows
endemic to the Euro-Mediterranean basin caused by
‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), a non
culturable plant pathogenic Mollicute. Bois noir symptoms
could be associated with ‘Ca. P. solani’ in two
Azerbaijanian vineyards where disease incidence and severity
were recorded for five local Vitis vinifera cultivars.
In order to gain insight into the epidemiology of Bois noir
in Azerbaijan, ‘Ca. P. solani’ isolates infecting plants were
characterized by multi-locus sequence analysis and their
secY and stamp gene sequences compared to that of the
strains detected in other plants and in local Cixiidae
planthoppers. Genotypes were determined for two nonribosomal
house-keeping genes, namely tuf and secY, as
well as two variable markers namely Stamp and mleP1
genes, that respectively encode the antigenic membrane
protein AMP and a 2-Hydroxycarboxylate transporter. The
Azerbaijanian BN phytoplasma isolates corresponded to
three tufB and secY genotypes. A finer differentiation of
Azerbaijanian ‘Ca. P. solani’ isolates was obtained with
mleP1 as five different mleP1 genetic variants were found.
Finally, Stamp gene allowed differentiating four new genotypes
in grapevine among the 10 new Stamp genotypes
detected in various plants in Azerbaijan. The preliminary
survey for infected insects conducted in northern Azerbaijan,
led to the identification of Hyalesthes obsoletus and
Reptalus noahi as potential vectors for two ‘Ca. P. solani’
new genotypes phylogenetically distant from the known
genetic clusters. Altogether these results indicate an important
genetic diversity of BN phytoplasmas in Azerbaijan
that certainly result from spread through local insect
vectors.
Plant Physiology and Biochemistry Journal, 2014
Water is a key factor influencing the yield and quality of crops. One of the parameters of plant ... more Water is a key factor influencing the yield and quality of crops. One of the parameters of plant biological
tolerance to constantly changing environmental conditions is the change of activities and numerous molecular
forms of antioxidant enzymes. Two durum (Triticum durum Desf.) wheat varieties contrasting for
drought tolerance, such as Barakatli-95 (drought tolerant) and Garagylchyg-2 (drought sensitive) were
grown over a wide area in the field. Experiments were carried out to study the effect of soil drought on
changes in activities and subcellular localization of superoxide dismutase isoforms. The levels of malondialdehyde,
glycine betaine and total proteins were also analyzed. The level of the enzyme activity appeared
to depend on thewheat varieties, duration of drought and stages of leaf development.Native polyacrylamide
gel electrophoresis (PAGE) revealed the presence of 9 isoenzymes of superoxide dismutase in wheat leaves
during drought.Mn-SODwas foundin the mitochondrial fractions, Fe-SODinthe chloroplast fraction andCu/
Zn-SODis localized in all subcellular fractions.Wheat leaves contain three different isoforms ofSOD(Mn-, Fe-
, Cu/Zn-SOD). Three isoforms of Mn-SOD, one isoform of Fe-SOD and five of Cu/Zn-SOD were observed in
wheat leaves using 3 mM KCN and 5 mM H2O2 as selective inhibitors. The expression of Mn-SOD was
preferentially enhanced by drought stress. It seems that Mn-SOD isoforms more than SOD ones play a major
role in the scavenging of superoxide radicals. The observed data showed that status of antioxidant enzymes
such as SOD could provide a meaningful tool for depicting drought tolerance of wheat genotype.
Drought tolerance is considered to be a quantitative trait manifesting complex phenotypic and gen... more Drought tolerance is considered to be a quantitative trait manifesting complex phenotypic
and genetic control (McWilliam 1989; Saleh et al. 2014). Because of global
environmental changes and increase in the world population, maintaining plant productivity
under drought conditions is of great importance (Takeda and Matsuoka
2008). Interest in research of physiological and biochemical processes improving
plant tolerance against adverse environmental factors has been increasing recently
(Bray et al. 2000; Wang et al. 2003). From this point of view the study of stress
effects on physiological and biochemical processes occurring in higher plants is
considered to be actual.
The main factor limiting wheat grain productivity is rust diseases. The study of the genetic basi... more The main factor limiting wheat grain productivity is rust diseases. The study of the genetic basis of plant resistance and the search of new and effective genes controlling resistance to disease, the selection of donors for breeding programs and the creation of new and improved wheat genotypes are always actual tasks aimed at enhancing the genetic diversity of crops. Brown (Puccinia recondita Rob.et Desm f. Tritici Eriks) and yellow (Puccinia striiformis f. Sp. Tritici) wheat rusts are the most common and dangerous diseases of wheat, which cause serious economic damage, reducing the yield and quality of grain. The use of genetically resistant varieties is the most effective, economically and environmentally reliable method of controlling diseases and reducing or eliminating the use of fungicides and minimizing crop losses from rust. *
Water Stress and Crop Plants: A Sustainable Approach, First Edition. Edited by Parvaiz Ahmad., 2016
Photosynthesis: Research for Food, Fuel and Future—15th International Conference on Photosynthesis
Present research is aimed to identify a Dreb1 gene in different wheat Triticum L. genotypes using... more Present research is aimed to identify a Dreb1 gene in different wheat Triticum L. genotypes using functional markers. A total of 12 wheat genotypes: 3 tetraploid (Triticum durum L., AABB, 2n = 4x = 28) and 9 hexaploid cultivars (Triticum aestivum L., AABBDD, 2n = 6x = 42) including tolerant, semi-tolerant and non-tolerant to drought were used.