Isabelle Noiseux - Academia.edu (original) (raw)

Papers by Isabelle Noiseux

Journal of international management studies, Mar 1, 2023

Pathogen detection and analysis is critical for medicine, food safety, agriculture, public health... more Pathogen detection and analysis is critical for medicine, food safety, agriculture, public health and biosecurity. Many current microbial detection approaches are based on century-old culturing methods which, while reliable, are slow, provide relatively little information about the ...

Journal of International Management Studies

A multi-center study has been set up to accurately characterize the optical properties of diffusi... more A multi-center study has been set up to accurately characterize the optical properties of diffusive liquid phantoms based on Intralipid and India ink at near-infrared (NIR) wavelengths. Nine research laboratories from six countries adopting different measurement techniques, instrumental setups , and data analysis methods determined at their best the optical properties and relative uncertainties of diffusive dilutions prepared with common samples of the two compounds. By exploiting a suitable statistical model, comprehensive reference values at three NIR wavelengths for the intrinsic absorption coefficient of India ink and the intrinsic reduced scattering coefficient of Intralipid-20% were determined with an uncertainty of about 2% or better, depending on the wavelength considered, and 1%, respectively. Even if in this study we focused on particular batches of India ink and Intralipid, the reference values determined here represent a solid and useful starting point for preparing diffusive liquid phantoms with accurately defined optical properties. Furthermore, due to the ready availability, low cost, long-term stability and batch-to-batch reproducibility of these compounds, they provide a unique fundamental tool for the calibration and performance assessment of diffuse optical spectroscopy instrumentation intended to be used in laboratory or clinical environment. Finally, the

Online Questionnaire: Inflammatory Bowel Disease Patient Perceptions of Diagnostic and Monitoring... more Online Questionnaire: Inflammatory Bowel Disease Patient Perceptions of Diagnostic and Monitoring Tests and Procedures. This online questionnaire was designed to better understand the concerns raised by the tests/procedures among IBD patients, the transfer of information from the physician to patients and the patientsâ understanding of these tests/procedures, as well as the rate of prescriptions, the reasons patients refuse to undergo such tests/procedures and the sociodemographic profile of the participants (Additional file 1- Online Questionnaire). (DOC 179 kb)

SPIE Proceedings, 2005

This paper presents a theoretical model of the effect of the geometry of illumination and collect... more This paper presents a theoretical model of the effect of the geometry of illumination and collection in fluorescent media, which exhibit self-absorption at sufficiently high concentrations. In order to derive a relation between the incident excitation intensity and the fluorescence ...

The effect of a magnetic field on the steady-state and time-resolved optical emission of a custom... more The effect of a magnetic field on the steady-state and time-resolved optical emission of a custom fullerene-linked photosensitizer (PS) in liposome cell phantoms was studied at various oxygen concentrations (0.19-190 microM). Zeeman splitting of the triplet state and hyperfine coupling, which control intersystem crossing between singlet and triplet states, are altered in the presence of low magnetic fields (B < 320 mT), perturbing the luminescence intensity and lifetime as compared to the triplet state at B = 0. Measurements of the luminescence intensity and lifetime were performed using a time-domain apparatus integrated with a magnet. We propose that by probing magnet-affected optical emissions, one can monitor the state of oxygenation throughout the course of photodynamic therapy. Since the magnetic field effect (MFE) operates primarily by affecting the radical ion pairs related to type I photodynamic action, the enhancement or suppression of the MFE can be used as a measure of the dynamic equilibrium between the type I and II photodynamic pathways. The unique photo-initiated charge-transfer properties of the PS used in this study allow it to serve as both cytotoxic agent and oxygen probe that can provide in situ dosimetric information at close to real time.

Biomedical Optics and 3-D Imaging, 2012

L. Spinelli,1 M. Botwicz,2 N. Zolek,2 M. Kacprzak,2 D. Milej,2 A. Liebert,2 U. Weigel,3 T. Durdur... more L. Spinelli,1 M. Botwicz,2 N. Zolek,2 M. Kacprzak,2 D. Milej,2 A. Liebert,2 U. Weigel,3 T. Durduran,3 F. Foschum,4 A. Kienle,4 F. Baribeau,5 S. Leclair,5 J.-P. Bouchard,5 I. Noiseux,5 P. Gallant,5 O. Mermut,5 A. Pifferi,1,6 A. Torricelli,6 R. Cubeddu,1,6 H.-C. Ho,7,8 M. Mazurenka,8 H. Wabnitz,8 K. Klauenberg,8 O. Bodnar,8 C. Elster,8 M. Bénazech-Lavoué,9 Y. Bérubé-Lauzière,9 F. Lesage,10 P. Di Ninni,11 F. Martelli,11 and G. Zaccanti11 1Consiglio Nazionale delle Ricerche–Istituto di Fotonica e Nanotecnologie, Milano, Italy 2IBIB, ...

Journal of Biomedical Optics, 2008

The time-resolved fluorescence of photosensitizers ͑PSs͒ of varying hydrophobicities, di-and tetr... more The time-resolved fluorescence of photosensitizers ͑PSs͒ of varying hydrophobicities, di-and tetrasulfonated Al phthalocyanines ͑Al-2 and Al-4͒, and Photochlor® ͑HPPH͒, was investigated in liposomes used as cell-mimetic models. Using frequency-and timedomain apparatus, the fluorescence lifetime, fluo , was compared for PSs free in aqueous solution and in a liposome-associated state at varied temperatures ͑25 to 78°C͒ and oxygen concentrations ͑0-190 M͒. The analysis of fluo revealed different decay behaviors for the free-solution and liposome-confined PSs, most significantly for the lipophilic HPPH. Hydrophilic PS drugs ͑Al-4, Al-2͒ were less affected by the liposomal confinement, depending on the relative hydrophilicity of the compound and the consequent localization in lipsomes. Changes in the emission decay due to confinement were detected as differences in the lifetime between the bulk solution and the liposome-localized PS in response to heating and deoxygenation. Specifically, hydrophilic Al-4 produced an identical lifetime trend as a function of temperature both in solu and in a liposome-confined state. Hydrophobic HPPH exhibited a fundamental transformation in its fluorescence decay kinetics, transitioning from a multiexponential ͑in free solution͒ to single-exponential ͑in liposome͒ decay. Deoxygenation resulted in a ubiquitous fluo increase for all PSs in free solution, while the opposite, a fluo decrease, occurred in all liposomal PSs.

Journal of Biomedical Optics, 2008

Preferential tumor localization and the aggregation state of photosensitizers ͑PSs͒ can depend on... more Preferential tumor localization and the aggregation state of photosensitizers ͑PSs͒ can depend on the hydrophilic/hydrophobic nature of the molecule and affect their phototoxicity. In this study, three PSs of different hydrophilicity are introduced in liposomes to understand the structure-photochemistry relationship of PSs in this cellular model system. Absorbance and fluorescence spectra of amphiphilic aluminum ͑III͒ phthalocyanine disulfonate chloride adjacent isomer ͑Al-2͒, hydrophilic aluminum ͑III͒ phthalocyanine chloride tetrasulfonic acid ͑Al-4͒, and lipophilic 2-͑1-hexyloxyethyl͒-2-devinyl pyropheophorbide ͑HPPH͒ are compared in a liposomal confined state with free PS in bulk solution. For fluorescence measurements, a broad range of concentrations of both bulk and liposomal confined PSs are examined to track the transition from monomers to dimers or higher order aggregates. Epifluorescence microscopy, absorbance, and fluorescence measurements all confirm different localization of the PSs in liposomes, depending on their hydrophilicity. In turn, the localization affects the aggregation of molecules inside the liposome cell model. Data obtained with such cellular models could be useful in optimizing the photochemical properties of photosensitizing drugs based on their structure-dependent interactions with cellular media and subcellular organelles.

Validation of imaging technologies is becoming increasingly important as imaging begins to take a... more Validation of imaging technologies is becoming increasingly important as imaging begins to take a role as a biomarker. Activities such as drug development, guided intervention, patient stratification, and therapy response assessment rely on very high measurements of sensitivity and specificity at the earliest possible time point. Imaging is capable of providing those measurements, but without validation of the results, clinical acceptance will be delayed. It must be pointed out, however, that validation is different from verification, and ...

BMC Gastroenterology, 2019

Background: Inflammatory Bowel Disease (IBD) with its high incidence and prevalence rates in Cana... more Background: Inflammatory Bowel Disease (IBD) with its high incidence and prevalence rates in Canada generates a heavy burden of tests and procedures. The purpose of this study is to gain a better understanding of the transfer of information from physician to patient, as well as the patient understanding and perceptions about the tests and procedures that are ordered to them in the context of IBD diagnosis and monitoring. Methods: An online questionnaire was completed by 210 IBD patients in Canada. Information on the five mostoften used tests or procedures in IBD diagnosis/monitoring was collected. These include: general blood test, colonoscopy, colon biopsy, medical imaging and stool testing. Results: The general blood test is both the most ordered and most refused tool. It is also the one with which patients are the least comfortable, the one that generates the least concern and the one about which physicians provide the least information. The stool test is the test/procedure with which patients are the most comfortable. Procedures raise more concerns among patients and physicians provide more information about why they are needed, their impact and the risks they present. Very little information is provided to patients about the risks of having false positives or negative tests. Conclusions: This study provides an initial understanding of patient perceptions, the transfer of information from a physician to a patient and a patient's understanding of the tests and procedures that will be required to treat IBD throughout what is a lifelong disease. The present study takes a first step in better understanding the acceptance of the test or procedure by IBD patients, which is essential for them to adhere to the monitoring process.

L'invention concerne un procede et un appareil de detection optique de contaminants residuels... more L'invention concerne un procede et un appareil de detection optique de contaminants residuels presents sur certains articles (par exemple des instruments et des equipements medicaux) apres leur lavage ou leur rincage dans une unite de lavage. Le systeme de detection de contaminants selon l'invention detecte le rayonnement lumineux emis par les contaminants en presence de lumiere ambiante pour donner une indication de la contamination des articles.

This project uses function-based detection via a fundamental understanding of the genetic markers... more This project uses function-based detection via a fundamental understanding of the genetic markers of AR to distinguish harmful organisms from innocuous ones. This approach circumvents complex analyses to unravel the taxonomic details of 1399 pathogen species, enormously simplifying detection requirements. Laval Hospital's fast permeabilization strategy enables AR revelation in <1hr. Packaging the AR protocols in liquid-processing cartridges and coupling these

The development of multimodal molecular probes and photosensitizing agents for use in photodynami... more The development of multimodal molecular probes and photosensitizing agents for use in photodynamic therapy (PDT) is vital for optimizing and monitoring cytotoxic responses. We propose a combinatorial approach utilizing photosensitizing molecules that are both paramagnetic and ...

Abstract Spin states of Type 1 photosensitizer radicals are perturbed using weak magnetic fields ... more Abstract Spin states of Type 1 photosensitizer radicals are perturbed using weak magnetic fields (< 200mT) to affect their luminescence, measured using time-domain photon counting. Magneto-photosensitization effects on photodynamic pathways in liposome cell phantoms ...

Progress in Biomedical Optics and Imaging, 2011

Optical tissue phantoms are very important tools for the development of biomedical imaging applic... more Optical tissue phantoms are very important tools for the development of biomedical imaging applications. Optical phantoms are often used as ground truth against which instruments results can be compared. It is therefore important that the optical properties of reference ...

Progress in Biomedical Optics and Imaging, Feb 1, 2010

We fabricated permanent solid polyurethane-based phantoms in which fluorophores were homogeneousl... more We fabricated permanent solid polyurethane-based phantoms in which fluorophores were homogeneously incorporated. For this study, fluorophores of three different families were used: Cyanines, Alexa Fluor and Quantum Dots. The goal of this study was to evaluate the impact of casting the ...

Journal of international management studies, Mar 1, 2023

Pathogen detection and analysis is critical for medicine, food safety, agriculture, public health... more Pathogen detection and analysis is critical for medicine, food safety, agriculture, public health and biosecurity. Many current microbial detection approaches are based on century-old culturing methods which, while reliable, are slow, provide relatively little information about the ...

Journal of International Management Studies

A multi-center study has been set up to accurately characterize the optical properties of diffusi... more A multi-center study has been set up to accurately characterize the optical properties of diffusive liquid phantoms based on Intralipid and India ink at near-infrared (NIR) wavelengths. Nine research laboratories from six countries adopting different measurement techniques, instrumental setups , and data analysis methods determined at their best the optical properties and relative uncertainties of diffusive dilutions prepared with common samples of the two compounds. By exploiting a suitable statistical model, comprehensive reference values at three NIR wavelengths for the intrinsic absorption coefficient of India ink and the intrinsic reduced scattering coefficient of Intralipid-20% were determined with an uncertainty of about 2% or better, depending on the wavelength considered, and 1%, respectively. Even if in this study we focused on particular batches of India ink and Intralipid, the reference values determined here represent a solid and useful starting point for preparing diffusive liquid phantoms with accurately defined optical properties. Furthermore, due to the ready availability, low cost, long-term stability and batch-to-batch reproducibility of these compounds, they provide a unique fundamental tool for the calibration and performance assessment of diffuse optical spectroscopy instrumentation intended to be used in laboratory or clinical environment. Finally, the

Online Questionnaire: Inflammatory Bowel Disease Patient Perceptions of Diagnostic and Monitoring... more Online Questionnaire: Inflammatory Bowel Disease Patient Perceptions of Diagnostic and Monitoring Tests and Procedures. This online questionnaire was designed to better understand the concerns raised by the tests/procedures among IBD patients, the transfer of information from the physician to patients and the patientsâ understanding of these tests/procedures, as well as the rate of prescriptions, the reasons patients refuse to undergo such tests/procedures and the sociodemographic profile of the participants (Additional file 1- Online Questionnaire). (DOC 179 kb)

SPIE Proceedings, 2005

This paper presents a theoretical model of the effect of the geometry of illumination and collect... more This paper presents a theoretical model of the effect of the geometry of illumination and collection in fluorescent media, which exhibit self-absorption at sufficiently high concentrations. In order to derive a relation between the incident excitation intensity and the fluorescence ...

The effect of a magnetic field on the steady-state and time-resolved optical emission of a custom... more The effect of a magnetic field on the steady-state and time-resolved optical emission of a custom fullerene-linked photosensitizer (PS) in liposome cell phantoms was studied at various oxygen concentrations (0.19-190 microM). Zeeman splitting of the triplet state and hyperfine coupling, which control intersystem crossing between singlet and triplet states, are altered in the presence of low magnetic fields (B < 320 mT), perturbing the luminescence intensity and lifetime as compared to the triplet state at B = 0. Measurements of the luminescence intensity and lifetime were performed using a time-domain apparatus integrated with a magnet. We propose that by probing magnet-affected optical emissions, one can monitor the state of oxygenation throughout the course of photodynamic therapy. Since the magnetic field effect (MFE) operates primarily by affecting the radical ion pairs related to type I photodynamic action, the enhancement or suppression of the MFE can be used as a measure of the dynamic equilibrium between the type I and II photodynamic pathways. The unique photo-initiated charge-transfer properties of the PS used in this study allow it to serve as both cytotoxic agent and oxygen probe that can provide in situ dosimetric information at close to real time.

Biomedical Optics and 3-D Imaging, 2012

L. Spinelli,1 M. Botwicz,2 N. Zolek,2 M. Kacprzak,2 D. Milej,2 A. Liebert,2 U. Weigel,3 T. Durdur... more L. Spinelli,1 M. Botwicz,2 N. Zolek,2 M. Kacprzak,2 D. Milej,2 A. Liebert,2 U. Weigel,3 T. Durduran,3 F. Foschum,4 A. Kienle,4 F. Baribeau,5 S. Leclair,5 J.-P. Bouchard,5 I. Noiseux,5 P. Gallant,5 O. Mermut,5 A. Pifferi,1,6 A. Torricelli,6 R. Cubeddu,1,6 H.-C. Ho,7,8 M. Mazurenka,8 H. Wabnitz,8 K. Klauenberg,8 O. Bodnar,8 C. Elster,8 M. Bénazech-Lavoué,9 Y. Bérubé-Lauzière,9 F. Lesage,10 P. Di Ninni,11 F. Martelli,11 and G. Zaccanti11 1Consiglio Nazionale delle Ricerche–Istituto di Fotonica e Nanotecnologie, Milano, Italy 2IBIB, ...

Journal of Biomedical Optics, 2008

The time-resolved fluorescence of photosensitizers ͑PSs͒ of varying hydrophobicities, di-and tetr... more The time-resolved fluorescence of photosensitizers ͑PSs͒ of varying hydrophobicities, di-and tetrasulfonated Al phthalocyanines ͑Al-2 and Al-4͒, and Photochlor® ͑HPPH͒, was investigated in liposomes used as cell-mimetic models. Using frequency-and timedomain apparatus, the fluorescence lifetime, fluo , was compared for PSs free in aqueous solution and in a liposome-associated state at varied temperatures ͑25 to 78°C͒ and oxygen concentrations ͑0-190 M͒. The analysis of fluo revealed different decay behaviors for the free-solution and liposome-confined PSs, most significantly for the lipophilic HPPH. Hydrophilic PS drugs ͑Al-4, Al-2͒ were less affected by the liposomal confinement, depending on the relative hydrophilicity of the compound and the consequent localization in lipsomes. Changes in the emission decay due to confinement were detected as differences in the lifetime between the bulk solution and the liposome-localized PS in response to heating and deoxygenation. Specifically, hydrophilic Al-4 produced an identical lifetime trend as a function of temperature both in solu and in a liposome-confined state. Hydrophobic HPPH exhibited a fundamental transformation in its fluorescence decay kinetics, transitioning from a multiexponential ͑in free solution͒ to single-exponential ͑in liposome͒ decay. Deoxygenation resulted in a ubiquitous fluo increase for all PSs in free solution, while the opposite, a fluo decrease, occurred in all liposomal PSs.

Journal of Biomedical Optics, 2008

Preferential tumor localization and the aggregation state of photosensitizers ͑PSs͒ can depend on... more Preferential tumor localization and the aggregation state of photosensitizers ͑PSs͒ can depend on the hydrophilic/hydrophobic nature of the molecule and affect their phototoxicity. In this study, three PSs of different hydrophilicity are introduced in liposomes to understand the structure-photochemistry relationship of PSs in this cellular model system. Absorbance and fluorescence spectra of amphiphilic aluminum ͑III͒ phthalocyanine disulfonate chloride adjacent isomer ͑Al-2͒, hydrophilic aluminum ͑III͒ phthalocyanine chloride tetrasulfonic acid ͑Al-4͒, and lipophilic 2-͑1-hexyloxyethyl͒-2-devinyl pyropheophorbide ͑HPPH͒ are compared in a liposomal confined state with free PS in bulk solution. For fluorescence measurements, a broad range of concentrations of both bulk and liposomal confined PSs are examined to track the transition from monomers to dimers or higher order aggregates. Epifluorescence microscopy, absorbance, and fluorescence measurements all confirm different localization of the PSs in liposomes, depending on their hydrophilicity. In turn, the localization affects the aggregation of molecules inside the liposome cell model. Data obtained with such cellular models could be useful in optimizing the photochemical properties of photosensitizing drugs based on their structure-dependent interactions with cellular media and subcellular organelles.

Validation of imaging technologies is becoming increasingly important as imaging begins to take a... more Validation of imaging technologies is becoming increasingly important as imaging begins to take a role as a biomarker. Activities such as drug development, guided intervention, patient stratification, and therapy response assessment rely on very high measurements of sensitivity and specificity at the earliest possible time point. Imaging is capable of providing those measurements, but without validation of the results, clinical acceptance will be delayed. It must be pointed out, however, that validation is different from verification, and ...

BMC Gastroenterology, 2019

Background: Inflammatory Bowel Disease (IBD) with its high incidence and prevalence rates in Cana... more Background: Inflammatory Bowel Disease (IBD) with its high incidence and prevalence rates in Canada generates a heavy burden of tests and procedures. The purpose of this study is to gain a better understanding of the transfer of information from physician to patient, as well as the patient understanding and perceptions about the tests and procedures that are ordered to them in the context of IBD diagnosis and monitoring. Methods: An online questionnaire was completed by 210 IBD patients in Canada. Information on the five mostoften used tests or procedures in IBD diagnosis/monitoring was collected. These include: general blood test, colonoscopy, colon biopsy, medical imaging and stool testing. Results: The general blood test is both the most ordered and most refused tool. It is also the one with which patients are the least comfortable, the one that generates the least concern and the one about which physicians provide the least information. The stool test is the test/procedure with which patients are the most comfortable. Procedures raise more concerns among patients and physicians provide more information about why they are needed, their impact and the risks they present. Very little information is provided to patients about the risks of having false positives or negative tests. Conclusions: This study provides an initial understanding of patient perceptions, the transfer of information from a physician to a patient and a patient's understanding of the tests and procedures that will be required to treat IBD throughout what is a lifelong disease. The present study takes a first step in better understanding the acceptance of the test or procedure by IBD patients, which is essential for them to adhere to the monitoring process.

L'invention concerne un procede et un appareil de detection optique de contaminants residuels... more L'invention concerne un procede et un appareil de detection optique de contaminants residuels presents sur certains articles (par exemple des instruments et des equipements medicaux) apres leur lavage ou leur rincage dans une unite de lavage. Le systeme de detection de contaminants selon l'invention detecte le rayonnement lumineux emis par les contaminants en presence de lumiere ambiante pour donner une indication de la contamination des articles.

This project uses function-based detection via a fundamental understanding of the genetic markers... more This project uses function-based detection via a fundamental understanding of the genetic markers of AR to distinguish harmful organisms from innocuous ones. This approach circumvents complex analyses to unravel the taxonomic details of 1399 pathogen species, enormously simplifying detection requirements. Laval Hospital's fast permeabilization strategy enables AR revelation in <1hr. Packaging the AR protocols in liquid-processing cartridges and coupling these

The development of multimodal molecular probes and photosensitizing agents for use in photodynami... more The development of multimodal molecular probes and photosensitizing agents for use in photodynamic therapy (PDT) is vital for optimizing and monitoring cytotoxic responses. We propose a combinatorial approach utilizing photosensitizing molecules that are both paramagnetic and ...

Abstract Spin states of Type 1 photosensitizer radicals are perturbed using weak magnetic fields ... more Abstract Spin states of Type 1 photosensitizer radicals are perturbed using weak magnetic fields (< 200mT) to affect their luminescence, measured using time-domain photon counting. Magneto-photosensitization effects on photodynamic pathways in liposome cell phantoms ...

Progress in Biomedical Optics and Imaging, 2011

Optical tissue phantoms are very important tools for the development of biomedical imaging applic... more Optical tissue phantoms are very important tools for the development of biomedical imaging applications. Optical phantoms are often used as ground truth against which instruments results can be compared. It is therefore important that the optical properties of reference ...

Progress in Biomedical Optics and Imaging, Feb 1, 2010

We fabricated permanent solid polyurethane-based phantoms in which fluorophores were homogeneousl... more We fabricated permanent solid polyurethane-based phantoms in which fluorophores were homogeneously incorporated. For this study, fluorophores of three different families were used: Cyanines, Alexa Fluor and Quantum Dots. The goal of this study was to evaluate the impact of casting the ...