Ivy Ramis - Academia.edu (original) (raw)

Papers by Ivy Ramis

Research paper thumbnail of Evaluation of the Speed-Oligo® Mycobacteria assay for the identification of nontuberculous mycobacteria

Journal of medical microbiology, Jan 16, 2015

Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly commo... more Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo® Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo® Mycobacteria for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates). Speed-Oligo® Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo® Mycobacteria and the original identification were re-assessed by the Speed-Oligo® Mycobacteria and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein encoding genes. We found 93.5% (58/62) of concordance for the identification of NTM as compared to the original id...

Research paper thumbnail of Antimicrobial susceptibility of rapidly growing mycobacteria using the rapid colorimetric method

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Jan 29, 2015

Drug susceptibility testing (DST) of rapidly growing mycobacteria (RGM) are recommended for guidi... more Drug susceptibility testing (DST) of rapidly growing mycobacteria (RGM) are recommended for guiding the antimicrobial therapy. We have evaluated the use of resazurin in Mueller-Hinton medium (MHR) for MIC determination of RGM and compared the results with those obtained with the reference standard broth microdilution in Mueller-Hinton (MH) and with the resazurin microtiter assay (REMA) in 7H9 broth. The MIC of eight drugs: amikacin (AMI), cefoxitin (FOX), ciprofloxacin (CIP), clarithromycin (CLA), doxycycline (DOX), linezolid (LZD), moxifloxacin (MXF) and trimethoprim-sulfamethoxazole (TMP-SMX) were evaluated against 76 RGM (18 species) using three methods (MH, MHR, and REMA) in a 96-well plate format incubated at 37 °C over 3-5 days. Results obtained in the MH plates were interpreted by the appearance of turbidity at the bottom of the well before adding the resazurin. MHR and 7H9-REMA plates were read by visual observation for a change in color from blue to pink. The majority of re...

Research paper thumbnail of Molecular basis of the pathogenicity in Helicobacter pylori clinical isolates

J Clin Microbiol, 2010

... Silveira Fernandes1, Raul Mendoza-Sassi1, Obirajara Rodrigues1, Carlos Renan Varela Juliano1,... more ... Silveira Fernandes1, Raul Mendoza-Sassi1, Obirajara Rodrigues1, Carlos Renan Varela Juliano1, Carlos James Scaini1, Pedro Eduardo Almeida da Silva1* 1. Laboratório de Biologia Molecular, Universidade Federal do Rio Grande, Rua ...

Research paper thumbnail of Avaliação Da Atividade Antimicobacteriana De Extratos Vegetais e Moléculas De Síntese

Vittalle Revista De Ciencias Da Saude, Jan 11, 2011

A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de ... more A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de casos. Considerando o aumento no número de casos por cepas multidrogarresistente (MDR-TB) e o reduzido arsenal terapêutico, faz-se necessária à busca de novas alternativas farmacológicas para o tratamento de TB. Os extratos vegetais tem sido uma fonte importante para o desenvolvimento de substâncias com atividade antimicrobiana, principalmente, em países ricos em biodiversidade, como é o caso do Brasil. Outra estrategia é a síntese de novas moléculas que podem ser desenhadas a partir de estruturas já conhecidas ou buscando atingir novos alvos. O grupo de pesquisa do Laboratório de Micobactérias/Biologia Molecular da FURG tem avaliado a atividade antibacteriana de extratos vegetais e moléculas sintéticas buscando novas alternativas terapêuticas para TB.

Research paper thumbnail of Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis

The Journal of Infection in Developing Countries, 2015

Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carc... more Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases. Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR. It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B. No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found. We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.

Research paper thumbnail of Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Diagnostic Microbiology and Infectious Disease, 2015

CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing n... more CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing numbers of repeat sequences (EPIYAs), which can result in a modulation of the inflammatory response. This study investigated whether the presence of CagA EPIYA variations in strains of H. pylori that are positive for this region contributes to differing degrees of disease severity in the gastric mucosa. In this study, 157 H. pylori-positive patients were included, and of those, 40.8% (64/157) were infected with cagA-positive strains, which were assayed for the presence of CagA EPIYA-ABC, EPIYA-ABCC, and EPIYA-ABCCC. Peptic ulcers were significantly more prevalent in patients infected with strains containing CagA EPIYA-ABCC/ABCCC than in those with CagA EPIYA ABC strains (P=0.044). This suggests that the number of repetitions of EPIYA-C influences the development of gastroduodenal lesions, highlighting the importance and usefulness of evaluating the cagA gene sequence when making therapeutic intervention decisions in patients infected with H. pylori.

Research paper thumbnail of Gene polymorphism of IL-6, IL-8 and IL-10 and the risk of gastric pathologies in patients infected with Helicobacter pylori

Journal of Microbiology, Immunology and Infection, 2015

Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-... more Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to identify the polymorphisms in the IL-6, IL-8, and IL-10 genes and their associations with H. pylori infection and gastric pathologies. Gastric biopsy samples of 151 patients infected with H. pylori and 76 uninfected individuals were used. Helicobacter pylori infection was diagnosed by histological examination and the detection of the ureA and glmM genes. The polymorphisms in the IL-6 (at position -174), IL-8 (at position -251), and IL-10 (at position -819) were detected by polymerase chain reaction-restriction fragment length polymorphism. Among the genetic polymorphisms studied, we observed that only the presence of the A allele at position -251 of the IL-8 gene was significantly associated with H. pylori infection. In addition, patient carriers of the A/A genotype at position -251 of the IL-8 gene and carriers of the T allele at position -819 of the IL-10 gene had an increased risk of peptic ulcer disease in the presence of H. pylori infection. We did not find a correlation between polymorphisms in the IL-6, IL-8, and IL-10 genes and a higher risk of gastric carcinoma. We demonstrated that polymorphisms in the IL-8 gene was significantly associated with H. pylori infection. Furthermore, polymorphisms in the IL-8 and IL-10 genes were associated with an enhanced risk of peptic ulcer disease in H. pylori-positive patients.

Research paper thumbnail of Authors reply: cagE as a biomarker of the pathogenicity of Helicobacter pylori

Revista da Sociedade Brasileira de Medicina Tropical

Revista da Sociedade Brasileira de Medicina Tropical http://dx.

Research paper thumbnail of Evaluation of the Speed-Oligo® Mycobacteria assay for the identification of nontuberculous mycobacteria

Journal of medical microbiology, Jan 16, 2015

Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly commo... more Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo® Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo® Mycobacteria for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates). Speed-Oligo® Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo® Mycobacteria and the original identification were re-assessed by the Speed-Oligo® Mycobacteria and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein encoding genes. We found 93.5% (58/62) of concordance for the identification of NTM as compared to the original id...

Research paper thumbnail of Improving tuberculosis control through the partnership between university and the health system

Revista da Sociedade Brasileira de Medicina Tropical, 2012

Qualificar o controle da tuberculose mediante a parceria entre a ABSTRACT Introduction: Tuberculo... more Qualificar o controle da tuberculose mediante a parceria entre a ABSTRACT Introduction: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. Methods: Direct microscopy of sputum and culture in Ogawa-Kudoh were performed on 1,636 samples from 787 patients. DST of positive cultures was performed by resazurin microtiter assay and genotyping by mycobacterial interspersed repetitive units-variable number tandem repeat. Results: A total 91 patients with TB were identified. The culture increased case detection by 32% compared with the microscopy; acquired resistance was 3.3% and the genotyping showed high genetic diversity. Conclusions: Ogawa-Kudoh contributed significantly to the increase in case detection and is suitable for implementation in poor-resource locations. The acquired resistance rate was lower than that reported in a recent Brazilian survey. The high genetic diversity is possibly related to the high TB prevalence in the population, as well as to early detection and suitable treatment of patients. The interaction between research and health care is important for reorienting the practice, transferring technology, and improving TB control.

Research paper thumbnail of Molecular Basis of Pathogenicity in Helicobacter pylori Clinical Isolates

Journal of Clinical Microbiology, 2010

This study identified pathogenicity genes in 40 Helicobacter pylori clinical isolates. The cagA, ... more This study identified pathogenicity genes in 40 Helicobacter pylori clinical isolates. The cagA, vacA, and iceA genes were detected in 65%, 97.5%, and 97.5% of the isolates, respectively. The cagA, iceA1, and vacAs1a/m1 genes were related to erosive gastritis, whereas the vacAs2/m2 and iceA2 genes were associated with enanthematous gastritis.

Research paper thumbnail of Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients

Brazilian Journal of Microbiology, 2012

Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted ... more Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma.

Research paper thumbnail of Análise Comparativa Entre Alvos Moleculares Para Detecçãodo Helicobacter Pylori

Revista de Epidemiologia e Controle de Infecção, 2016

Research paper thumbnail of Evaluation of the Speed-Oligo® Mycobacteria assay for the identification of nontuberculous mycobacteria

Journal of medical microbiology, Jan 16, 2015

Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly commo... more Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo® Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo® Mycobacteria for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates). Speed-Oligo® Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo® Mycobacteria and the original identification were re-assessed by the Speed-Oligo® Mycobacteria and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein encoding genes. We found 93.5% (58/62) of concordance for the identification of NTM as compared to the original id...

Research paper thumbnail of Antimicrobial susceptibility of rapidly growing mycobacteria using the rapid colorimetric method

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Jan 29, 2015

Drug susceptibility testing (DST) of rapidly growing mycobacteria (RGM) are recommended for guidi... more Drug susceptibility testing (DST) of rapidly growing mycobacteria (RGM) are recommended for guiding the antimicrobial therapy. We have evaluated the use of resazurin in Mueller-Hinton medium (MHR) for MIC determination of RGM and compared the results with those obtained with the reference standard broth microdilution in Mueller-Hinton (MH) and with the resazurin microtiter assay (REMA) in 7H9 broth. The MIC of eight drugs: amikacin (AMI), cefoxitin (FOX), ciprofloxacin (CIP), clarithromycin (CLA), doxycycline (DOX), linezolid (LZD), moxifloxacin (MXF) and trimethoprim-sulfamethoxazole (TMP-SMX) were evaluated against 76 RGM (18 species) using three methods (MH, MHR, and REMA) in a 96-well plate format incubated at 37 °C over 3-5 days. Results obtained in the MH plates were interpreted by the appearance of turbidity at the bottom of the well before adding the resazurin. MHR and 7H9-REMA plates were read by visual observation for a change in color from blue to pink. The majority of re...

Research paper thumbnail of Molecular basis of the pathogenicity in Helicobacter pylori clinical isolates

J Clin Microbiol, 2010

... Silveira Fernandes1, Raul Mendoza-Sassi1, Obirajara Rodrigues1, Carlos Renan Varela Juliano1,... more ... Silveira Fernandes1, Raul Mendoza-Sassi1, Obirajara Rodrigues1, Carlos Renan Varela Juliano1, Carlos James Scaini1, Pedro Eduardo Almeida da Silva1* 1. Laboratório de Biologia Molecular, Universidade Federal do Rio Grande, Rua ...

Research paper thumbnail of Avaliação Da Atividade Antimicobacteriana De Extratos Vegetais e Moléculas De Síntese

Vittalle Revista De Ciencias Da Saude, Jan 11, 2011

A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de ... more A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de casos. Considerando o aumento no número de casos por cepas multidrogarresistente (MDR-TB) e o reduzido arsenal terapêutico, faz-se necessária à busca de novas alternativas farmacológicas para o tratamento de TB. Os extratos vegetais tem sido uma fonte importante para o desenvolvimento de substâncias com atividade antimicrobiana, principalmente, em países ricos em biodiversidade, como é o caso do Brasil. Outra estrategia é a síntese de novas moléculas que podem ser desenhadas a partir de estruturas já conhecidas ou buscando atingir novos alvos. O grupo de pesquisa do Laboratório de Micobactérias/Biologia Molecular da FURG tem avaliado a atividade antibacteriana de extratos vegetais e moléculas sintéticas buscando novas alternativas terapêuticas para TB.

Research paper thumbnail of Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis

The Journal of Infection in Developing Countries, 2015

Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carc... more Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases. Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR. It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B. No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found. We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.

Research paper thumbnail of Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Diagnostic Microbiology and Infectious Disease, 2015

CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing n... more CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing numbers of repeat sequences (EPIYAs), which can result in a modulation of the inflammatory response. This study investigated whether the presence of CagA EPIYA variations in strains of H. pylori that are positive for this region contributes to differing degrees of disease severity in the gastric mucosa. In this study, 157 H. pylori-positive patients were included, and of those, 40.8% (64/157) were infected with cagA-positive strains, which were assayed for the presence of CagA EPIYA-ABC, EPIYA-ABCC, and EPIYA-ABCCC. Peptic ulcers were significantly more prevalent in patients infected with strains containing CagA EPIYA-ABCC/ABCCC than in those with CagA EPIYA ABC strains (P=0.044). This suggests that the number of repetitions of EPIYA-C influences the development of gastroduodenal lesions, highlighting the importance and usefulness of evaluating the cagA gene sequence when making therapeutic intervention decisions in patients infected with H. pylori.

Research paper thumbnail of Gene polymorphism of IL-6, IL-8 and IL-10 and the risk of gastric pathologies in patients infected with Helicobacter pylori

Journal of Microbiology, Immunology and Infection, 2015

Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-... more Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to identify the polymorphisms in the IL-6, IL-8, and IL-10 genes and their associations with H. pylori infection and gastric pathologies. Gastric biopsy samples of 151 patients infected with H. pylori and 76 uninfected individuals were used. Helicobacter pylori infection was diagnosed by histological examination and the detection of the ureA and glmM genes. The polymorphisms in the IL-6 (at position -174), IL-8 (at position -251), and IL-10 (at position -819) were detected by polymerase chain reaction-restriction fragment length polymorphism. Among the genetic polymorphisms studied, we observed that only the presence of the A allele at position -251 of the IL-8 gene was significantly associated with H. pylori infection. In addition, patient carriers of the A/A genotype at position -251 of the IL-8 gene and carriers of the T allele at position -819 of the IL-10 gene had an increased risk of peptic ulcer disease in the presence of H. pylori infection. We did not find a correlation between polymorphisms in the IL-6, IL-8, and IL-10 genes and a higher risk of gastric carcinoma. We demonstrated that polymorphisms in the IL-8 gene was significantly associated with H. pylori infection. Furthermore, polymorphisms in the IL-8 and IL-10 genes were associated with an enhanced risk of peptic ulcer disease in H. pylori-positive patients.

Research paper thumbnail of Authors reply: cagE as a biomarker of the pathogenicity of Helicobacter pylori

Revista da Sociedade Brasileira de Medicina Tropical

Revista da Sociedade Brasileira de Medicina Tropical http://dx.

Research paper thumbnail of Evaluation of the Speed-Oligo® Mycobacteria assay for the identification of nontuberculous mycobacteria

Journal of medical microbiology, Jan 16, 2015

Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly commo... more Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo® Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo® Mycobacteria for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates). Speed-Oligo® Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo® Mycobacteria and the original identification were re-assessed by the Speed-Oligo® Mycobacteria and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein encoding genes. We found 93.5% (58/62) of concordance for the identification of NTM as compared to the original id...

Research paper thumbnail of Improving tuberculosis control through the partnership between university and the health system

Revista da Sociedade Brasileira de Medicina Tropical, 2012

Qualificar o controle da tuberculose mediante a parceria entre a ABSTRACT Introduction: Tuberculo... more Qualificar o controle da tuberculose mediante a parceria entre a ABSTRACT Introduction: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. Methods: Direct microscopy of sputum and culture in Ogawa-Kudoh were performed on 1,636 samples from 787 patients. DST of positive cultures was performed by resazurin microtiter assay and genotyping by mycobacterial interspersed repetitive units-variable number tandem repeat. Results: A total 91 patients with TB were identified. The culture increased case detection by 32% compared with the microscopy; acquired resistance was 3.3% and the genotyping showed high genetic diversity. Conclusions: Ogawa-Kudoh contributed significantly to the increase in case detection and is suitable for implementation in poor-resource locations. The acquired resistance rate was lower than that reported in a recent Brazilian survey. The high genetic diversity is possibly related to the high TB prevalence in the population, as well as to early detection and suitable treatment of patients. The interaction between research and health care is important for reorienting the practice, transferring technology, and improving TB control.

Research paper thumbnail of Molecular Basis of Pathogenicity in Helicobacter pylori Clinical Isolates

Journal of Clinical Microbiology, 2010

This study identified pathogenicity genes in 40 Helicobacter pylori clinical isolates. The cagA, ... more This study identified pathogenicity genes in 40 Helicobacter pylori clinical isolates. The cagA, vacA, and iceA genes were detected in 65%, 97.5%, and 97.5% of the isolates, respectively. The cagA, iceA1, and vacAs1a/m1 genes were related to erosive gastritis, whereas the vacAs2/m2 and iceA2 genes were associated with enanthematous gastritis.

Research paper thumbnail of Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients

Brazilian Journal of Microbiology, 2012

Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted ... more Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma.

Research paper thumbnail of Análise Comparativa Entre Alvos Moleculares Para Detecçãodo Helicobacter Pylori

Revista de Epidemiologia e Controle de Infecção, 2016