Julieth Zapata Ortiz - Academia.edu (original) (raw)
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Instituto Interamericano de Cooperación para la Agricult
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Thesis Chapters by Julieth Zapata Ortiz
Despite the effort to promote planting of arazá (Eugenia stipitata) and Cocona (Solanum sessilifl... more Despite the effort to promote planting of arazá (Eugenia stipitata) and Cocona
(Solanum sessiliflorum), promising fruit of the Colombian Amazon, their culture is
relegated to profitable options, a situation that negatively affects their conservation.
In response to this problem, was studied the genetic variability of cultivated
resources of cocona and arazá, from ISSR molecular markers. 70 arazá (Eugenia
stipitata) accessions and 12 cocona (Solanum sessiliflorum) accessions were
analyzed, samples were collected in Caquetá and Guaviare and assessed using
Inter Simple Sequence Repeat (ISSR) markers to understand their genetic
variability. Genomic DNA was extracted from young leaves according to the
modified CTAB method, following the procedure described by Doyle and Doyle
(1987). Nine ISSR primers yielded 151 bands in arazá, and 17 ISSR primers
yielded 194 bands in cocona, showing that ISSR marker was efficient to analyze
the genetic diversity of these cultivars. The Jaccard similarity coefficient and the
cluster analysis with UPGMA method show that there is a wide genetic variability
betweenE. stipitata and S. sessiliflorum cultivars, possibly because to its early
domestication and the allogamous character of both species. Correspondence
between groupings and morphological patterns features was not observed. The
Principal Coordinate Analysis (PCoA) results indicated that in both species the
geographic precedence influence the differentiation of analyzed materials. The
study of the genetic variability of the arazá and cocona constitutes a necessary
step that can be exploited in the programs of improvement of this marginalized
species.
Despite the effort to promote planting of arazá (Eugenia stipitata) and Cocona (Solanum sessilifl... more Despite the effort to promote planting of arazá (Eugenia stipitata) and Cocona
(Solanum sessiliflorum), promising fruit of the Colombian Amazon, their culture is
relegated to profitable options, a situation that negatively affects their conservation.
In response to this problem, was studied the genetic variability of cultivated
resources of cocona and arazá, from ISSR molecular markers. 70 arazá (Eugenia
stipitata) accessions and 12 cocona (Solanum sessiliflorum) accessions were
analyzed, samples were collected in Caquetá and Guaviare and assessed using
Inter Simple Sequence Repeat (ISSR) markers to understand their genetic
variability. Genomic DNA was extracted from young leaves according to the
modified CTAB method, following the procedure described by Doyle and Doyle
(1987). Nine ISSR primers yielded 151 bands in arazá, and 17 ISSR primers
yielded 194 bands in cocona, showing that ISSR marker was efficient to analyze
the genetic diversity of these cultivars. The Jaccard similarity coefficient and the
cluster analysis with UPGMA method show that there is a wide genetic variability
betweenE. stipitata and S. sessiliflorum cultivars, possibly because to its early
domestication and the allogamous character of both species. Correspondence
between groupings and morphological patterns features was not observed. The
Principal Coordinate Analysis (PCoA) results indicated that in both species the
geographic precedence influence the differentiation of analyzed materials. The
study of the genetic variability of the arazá and cocona constitutes a necessary
step that can be exploited in the programs of improvement of this marginalized
species.