James Stangoulis - Academia.edu (original) (raw)

Papers by James Stangoulis

Journal of Plant Interactions, 2016

Horticulture, Environment, and Biotechnology, 2016

Journal of the Science of Food and Agriculture, 2007

... Diploid (2n = 2x = 24) and tetraploid (2n = 4x = 48) cultivated potatoes can readily be cross... more ... Diploid (2n = 2x = 24) and tetraploid (2n = 4x = 48) cultivated potatoes can readily be crossed with commercial potato (Tuberosum; 2n = 4x = 48) and represent a valuable gene pool for broadening its genetic base and enhancing ... Sirina 18.5b 14.3ab 28.5 18.7ab 13.7b 30.4 ...

Crop Science, May 1, 2011

Physiologia Plantarum, Jun 19, 2012

Scientific Reports, 2016

More than two billion people are micronutrient deficient. Polished grains of popular rice varieti... more More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g(-1) iron (Fe) and 16 μg g(-1 )zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g(-1 )Fe and 28 μg g(-1 )Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g(-1) in glasshouse settings; in contrast, under field conditions, 4 μg g(-1) was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g(-1 )Fe and 45.7 μg g(-1 )Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.

Boron (B) is an essential trace element for higher plants yet it becomes toxic when present at hi... more Boron (B) is an essential trace element for higher plants yet it becomes toxic when present at high concentrations. The site of B toxicity in plant roots is located in the root tip, and B tolerance is associated with the concentration of reducing sugars (RS) in this region. In the study reported here, we aimed to detect and map loci associated with this trait using a barley doubled-haploid population derived from a cross between Clipper (B sensitive) and Sahara 3771 (B tolerant). The population was screened at high B supply using solution culture, with the RS concentration measured in the root tip. The RS concentration varied widely in the parents and DH lines, ranging from 14 to 141 mg g-1. Using a linkage map of 420 molecular markers, quantitative trait loci (QTL) were detected on chromosomes 2H, 3H, 5H and 6H. Of these, the QTL on 2H had the largest effect, explaining 16% of the phenotypic variance, with the (high RS) allele contributed from Sahara 3771. QTLs detected here were c...

Journal of Food Composition and Analysis, 2015

Boron in Plant and Animal Nutrition, 2002

Horticulture, Environment, and Biotechnology, 2011

Future forecasts for climate change predict the global mean surface air temperature rise by 1 -4 ... more Future forecasts for climate change predict the global mean surface air temperature rise by 1 -4 and double current atmospheric CO2 level before the end of 21 century. Increased atmospheric temperature and CO2 concentration are particularly important concerns for agricultural, horticultural and native plant production. In this study, effects of long-term exposure to elevated temperature and carbon dioxide (CO2) on the growth and physiological responses of 3 cultivars of Chinese radish (Raphanus sativus L.) and 3 cultivars of Chinese cabbage (Brassica campestris L.) were examined. In result, the radishes exposed to elevated CO2 for 90 days after sowing (DAS) resulted in little or no change in the root dry weights and the rate of photosynthesis compared with those grown in ambient levels of CO2. In contrast, long-term exposure to elevated CO2 in cabbage had variable effects on the leaf dry weight. As a result of acclimating to the elevated temperature, the radish 'Chunha' had a higher rate of photosynthesis, stomatal conductance and internal CO2 concentration than in the control condition. Furthermore, the long-term exposure to a combination condition of elevated temperature and CO2 increased root dry weights of the radishes 'Cheongdae' and 'Chunha' more than elevated temperature alone. The combination of elevated CO2 and temperature stimulated the growth of roots more than that of shoots in the radish 'Chunha', and thus may have led a higher rate of nutrient uptake than other radish cultivars. In contrast, when the cabbage 'Chun-gwang' was exposed to a combination of elevated temperature and CO2 for 90 DAS, the leaf dry weight decreased about 3-fold more than that only exposed to elevated CO2 with drastic decreases in stomatal conductance, internal CO2 and photosynthesis rate. When the cabbage 'Samjin' was exposed to either elevated temperature alone or both elevated temperature and CO2 for 80 DAS, the decrease in the leaf dry weight was less than that of the other cabbage cultivars. Results indicated that the radish 'Chunha' and the cabbage 'Samjin' tolerated either elevated temperature alone or combination condition of elevated temperature and CO2 more than other cultivars.

PloS one, 2011

Rice is the primary source of food for billions of people in developing countries, yet the common... more Rice is the primary source of food for billions of people in developing countries, yet the commonly consumed polished grain contains insufficient levels of the key micronutrients iron (Fe), zinc (Zn) and Vitamin A to meet daily dietary requirements. Experts estimate that a rice-based diet should contain 14.5 µg g(-1) Fe in endosperm, the main constituent of polished grain, but breeding programs have failed to achieve even half of that value. Transgenic efforts to increase the Fe concentration of rice endosperm include expression of ferritin genes, nicotianamine synthase genes (NAS) or ferritin in conjunction with NAS genes, with results ranging from two-fold increases via single-gene approaches to six-fold increases via multi-gene approaches, yet no approach has reported 14.5 µg g(-1) Fe in endosperm. Three populations of rice were generated to constitutively overexpress OsNAS1, OsNAS2 or OsNAS3, respectively. Nicotianamine, Fe and Zn concentrations were significantly increased in u...

Postharvest Biology and Technology, 2014

Capsicum (Capsicum annuum L. cv. Aries) is a non-climacteric bell-pepper fruit, exhibiting limite... more Capsicum (Capsicum annuum L. cv. Aries) is a non-climacteric bell-pepper fruit, exhibiting limited ethylene and respiration levels during ripening. In contrast to climacteric fruit, such as tomato which is largely dependent upon ethylene to ripen, the regulation of non-climacteric ripening is still inadequately understood. A metabolomics approach was used to identify differentially abundant compounds between ripening stages with the aim of elucidating metabolic pathways involved in the regulation of nonclimacteric ripening. Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) was initially employed to screen potential metabolite differences among three ripening stages (Green, Breaker Red 1 and Light Red). Targeted analyses using liquid chromatography-mass spectrometry (LC-MS) or enzymatic assays were subsequently employed to characterise selected metabolites in more ripening stages. Starch, sugars and their derivatives were significantly modified during ripening which may affect the abundance of some glycolysis intermediates and consequently other metabolic pathways involving amino acids, colour and pungency precursors, and tricarboxylic acid (TCA) cycle intermediates. Furthermore, metabolites closely related to ethylene production such as cysteine and methionine gradually increased between the ripening stages, whereas putrescine significantly decreased during ripening, suggesting that some parts of the ethylene pathway may still be functional in this non-climacteric fruit. Thus, this study which utilised both profiling and targeted metabolomics, has identified a wide range of metabolites which are involved in various biochemical pathways and highlights the overall metabolic shifts during non-climacteric capsicum ripening.

PLoS ONE, 2011

Background: Rice is the primary source of food for billions of people in developing countries, ye... more Background: Rice is the primary source of food for billions of people in developing countries, yet the commonly consumed polished grain contains insufficient levels of the key micronutrients iron (Fe), zinc (Zn) and Vitamin A to meet daily dietary requirements. Experts estimate that a rice-based diet should contain 14.5 mg g 21 Fe in endosperm, the main constituent of polished grain, but breeding programs have failed to achieve even half of that value. Transgenic efforts to increase the Fe concentration of rice endosperm include expression of ferritin genes, nicotianamine synthase genes (NAS) or ferritin in conjunction with NAS genes, with results ranging from two-fold increases via single-gene approaches to six-fold increases via multi-gene approaches, yet no approach has reported 14.5 mg g 21 Fe in endosperm.

Physiologia Plantarum, 2013

Journal of Plant Nutrition and Soil Science, 2014

Zinc (Zn) deficiency is a well-documented problem in plants, causing decreased yields and nutriti... more Zinc (Zn) deficiency is a well-documented problem in plants, causing decreased yields and nutritional quality. When facing a shortage in Zn supply, plants acclimatize by enhancing the Zn acquisition. In this review, we highlight recent progress in understanding of plant resistance to Zn deficiency and discuss the future challenges to fully unravel its molecular basis of regulation. Emphasis is given on the physiological and molecular basis of Zn acquisition, the long-distance transport of Zn and the genotypic variations in Zn use efficiency of plants. Prospects of Zn biofortification strategies as well as further efforts for crop improvement to overcome Zn deficiency are also addressed.

Journal of Cereal Science, 2012

Free-Air Carbon dioxide Enrichment (FACE) Grain quality Nutrient and anti-nutrient factors Bread ... more Free-Air Carbon dioxide Enrichment (FACE) Grain quality Nutrient and anti-nutrient factors Bread quality a b s t r a c t We investigated wheat (Triticum aestivum) grain quality under Free Air CO 2 Enrichment (FACE) of 550 AE 10% CO 2 mmol mol À1 . In each of two full growing seasons (2008 and 2009), two times of sowing were compared, with late sowing designed to mimic high temperature during grain filling. Grain samples were subjected to a range of physical, nutritional and rheological quality assessments. Elevated CO 2 increased thousand grain weight (8%) and grain diameter (5%). Flour protein concentration was reduced by 11% at e[CO 2 ], with the highest reduction being observed at the late time of sowing in 2009, (15%). Most of the grain mineral concentrations decreased under e[CO 2 ] -Ca (11%), Mg (7%), P (11%) and S (7%), Fe (10%), Zn (17%), Na (19%), while total uptake of these nutrients per unit ground area increased. Rheological properties of the flour were altered by e[CO 2 ] and bread volume reduced by 7%. Phytate concentration in grains tended to decrease (17%) at e[CO 2 ] while grain fructan concentration remained unchanged. The data suggest that rising atmospheric [CO 2 ] will reduce the nutritional and rheological quality of wheat grain, but at high temperature, e[CO 2 ] effects may be moderated. Reduced phytate concentrations at e[CO 2 ] may improve bioavailability of Fe and Zn in wheat grain.

Functional Plant Biology, 2013

Capsicum (Capsicum annuum L.) is categorised as a non-climacteric fruit that exhibits limited eth... more Capsicum (Capsicum annuum L.) is categorised as a non-climacteric fruit that exhibits limited ethylene production during ripening and the molecular mechanisms associated with this process are poorly understood. A proteomic approach was used to identify the differentially expressed proteins during various ripening stages (Green (G), Breaker Red 1 (BR1) and Light Red (LR)) and the genes associated with their synthesis. From 2D gel electrophoresis (2DGE), seven protein spots were identified as selectively present either in G or BR1 and are involved in carbon metabolism, colour and fruit development, protein synthesis and chaperones or biosynthesis of amino acids and polyamines. One candidate of interest, 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) is known to be involved in ethylene biosynthesis and was only present in BR1 and is related to the tomato ACO isoform 4 (LeACO4) and hence named CaACO4. CaACO4 RNA expression as well as total ACO protein expression in multiple stages of ripening (G, Breaker (B), BR1, Breaker Red 2 (BR2), LR and Deep Red (DR)) corresponded to the 2DGE protein spot abundance in breaker stages. Our findings highlight the involvement of the ethylene pathway in non-climacteric fruit ripening.