Jaryl Kate Dinamling - Academia.edu (original) (raw)
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Objective: It has been reported that the aqueous extracts of Psidium guajava and Piper betle leav... more Objective: It has been reported that the aqueous extracts of Psidium guajava and Piper betle leaves showed anti-plaque activities during the early stages of dental plaque formation. The aim of the study was to elucidate if such anti-plaque activities involve any ultra-structural changes to the morphology of three early dental plaque bacteria, Streptococcus sanguinis, Streptococcus mitis and Actinomyces sp. Methodology: Pure cultures of the bacteria were suspended in BHI medium and treated with the test herbal extracts at the sub-lethal concentrations. The growth mixtures were incubated at 37°C. At the logarithmic growth phase (t 1 ), aliquots of 1 ml of the growth mixtures were fixed and used in the preparation of specimens for SEM studies. Ultra-structural alterations to the morphology of the treated cells noted were compared to those of the cells cultured under untreated conditions. Results: Following exposure of the bacteria to the two test herbal extracts, profound ultra-structural changes to their morphology were observed. The observed structural or morphological alterations could attribute to the bacteria being less active in performing normal physiological metabolic functions and thus rendering them less efficient to multiply. The changes noted included (i) reduced sizes of the bacteria, and (ii) majority cells at the non-dividing state as compared to those cultured under controlled conditions. Conclusions: This study has shown anti-plaque effects of aqueous extract of both P. betle and P. guajava. Fathilah AR, Yusoff M, Rahim ZHA. The Effect of Psidium guajava and Piper betle extracts on the Morphology of Dental Plaque Bacteria. Pak J Med Sci 2009;25(6):928-933.
Pakistan Journal of Biological Sciences, 2009
Journal of Oral Science, 2006
The adhesion of early settlers of dental plaque to the tooth surface has a role in the initiation... more The adhesion of early settlers of dental plaque to the tooth surface has a role in the initiation of the development of dental plaque. The hydrophobic surface properties of the bacteria cell wall are indirectly responsible for the adhesion of the bacteria cell to the acquired pellicle on the tooth surfaces. In this study, the effect of aqueous extract of two plants (Psidium guajava and Piper betle) on the cell-surface hydro-phobicity of early settlers of dental plaque was determined in vitro. Hexadecane, a hydrocarbon was used to represent the hydrophobic surface of the teeth in the oral cavity. It was found that treatment of the early plaque settlers with 1 mg/ml extract of Psidium guajava reduced the cell-surface hydrophobicity of Strep. sanguinis, Strep. mitis and Actinomyces sp. by 54.1%, 49.9% and 40.6%, respectively. Treatment of these bacteria with the same concentration of Piper betle however, showed a comparatively lesser effect (< 10%). It was also observed that the anti-adhesive effect of the two extracts on the binding of the early plaque settlers to hexadecane is concentration dependent.
Objective: It has been reported that the aqueous extracts of Psidium guajava and Piper betle leav... more Objective: It has been reported that the aqueous extracts of Psidium guajava and Piper betle leaves showed anti-plaque activities during the early stages of dental plaque formation. The aim of the study was to elucidate if such anti-plaque activities involve any ultra-structural changes to the morphology of three early dental plaque bacteria, Streptococcus sanguinis, Streptococcus mitis and Actinomyces sp. Methodology: Pure cultures of the bacteria were suspended in BHI medium and treated with the test herbal extracts at the sub-lethal concentrations. The growth mixtures were incubated at 37°C. At the logarithmic growth phase (t 1 ), aliquots of 1 ml of the growth mixtures were fixed and used in the preparation of specimens for SEM studies. Ultra-structural alterations to the morphology of the treated cells noted were compared to those of the cells cultured under untreated conditions. Results: Following exposure of the bacteria to the two test herbal extracts, profound ultra-structural changes to their morphology were observed. The observed structural or morphological alterations could attribute to the bacteria being less active in performing normal physiological metabolic functions and thus rendering them less efficient to multiply. The changes noted included (i) reduced sizes of the bacteria, and (ii) majority cells at the non-dividing state as compared to those cultured under controlled conditions. Conclusions: This study has shown anti-plaque effects of aqueous extract of both P. betle and P. guajava. Fathilah AR, Yusoff M, Rahim ZHA. The Effect of Psidium guajava and Piper betle extracts on the Morphology of Dental Plaque Bacteria. Pak J Med Sci 2009;25(6):928-933.
Pakistan Journal of Biological Sciences, 2009
Journal of Oral Science, 2006
The adhesion of early settlers of dental plaque to the tooth surface has a role in the initiation... more The adhesion of early settlers of dental plaque to the tooth surface has a role in the initiation of the development of dental plaque. The hydrophobic surface properties of the bacteria cell wall are indirectly responsible for the adhesion of the bacteria cell to the acquired pellicle on the tooth surfaces. In this study, the effect of aqueous extract of two plants (Psidium guajava and Piper betle) on the cell-surface hydro-phobicity of early settlers of dental plaque was determined in vitro. Hexadecane, a hydrocarbon was used to represent the hydrophobic surface of the teeth in the oral cavity. It was found that treatment of the early plaque settlers with 1 mg/ml extract of Psidium guajava reduced the cell-surface hydrophobicity of Strep. sanguinis, Strep. mitis and Actinomyces sp. by 54.1%, 49.9% and 40.6%, respectively. Treatment of these bacteria with the same concentration of Piper betle however, showed a comparatively lesser effect (< 10%). It was also observed that the anti-adhesive effect of the two extracts on the binding of the early plaque settlers to hexadecane is concentration dependent.