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Papers by Javier Obando-Ulloa

Crop Science, 1994

Plant breeders have an interest in the identification of genomic regions associated with the expr... more Plant breeders have an interest in the identification of genomic regions associated with the expression of quantitative traits because they recognize that such information could be used to improve realized heritability and reduce time per cycle of selection. The development of molecular marker technologies may provide tools to accomplish these goals. Maize (Zea mays L.) breeders develop new inbred parents of hybrids through toperossed and per se evaluation of numerous quantitative traits in segregating progeny from planned breeding crosses. This study was conducted to determine if regions of the genome associated with variability of agronomicaily important traits could be identified by a small, but typical, sample of toperossed and F~:4 progeny from the cross B73×MOIT. We identified genomic regions in 24 agronomic traits, using interval mapping and simultaneous estimation of multiple quantitative trait locus models. The estimated numbers of quantitative trait loci (QTL) identified per trait were about three five. Many of the identified QTL were the same for correlated traits. Interestingly, yield QTL were not in the same regions previously reported for B73×MO17. This comparison contains a number of potential confounding factors: source of parental inbreds, type of progeny, different genotype × environment interaction effects, and different small samples of progeny from the cross. Consideration of these factors and the power of the tests to identify QTL suggests that the sampling of progeny is the most likely explanation for the differences.

Around the world, the agroindustrial sector is highly dependent on the fossil fuels for its produ... more Around the world, the agroindustrial sector is highly dependent on the fossil fuels for its production process. Therefore, there is an urgent need to find new alternatives of energy resources in order to replace this dependence and make the process more efficient, support the production process of small and medium-sized farmers with clean technologies and achieve the mitigation of the greenhouse gases to contribute to diminish the effect of the climate change from a local initiative. The aim of this research was to evaluate the implementation of a thermal hybrid forced system (LP gas solar hybrid system) in two agricultural production units for the milk pasteurization to process fresh cheese for the local market. The system was implemented in two milk factories in the Northern Huetar Region of Costa Rica. This area is characterized by its high cloudiness and high volume precipitation, aspects that have limited the use of this alternative technology through the years. The system repl...

Postharvest Biology and Technology, 2009

This paper characterizes the changes in aroma volatiles associated with fruit senescence based on... more This paper characterizes the changes in aroma volatiles associated with fruit senescence based on the climacteric or non-climacteric behavior found in a collection of near-isogenic lines (NILs) of melon (Cucumis melo L.). Data were analyzed by univariate and multivariate methodologies. In general, senescence led to a decrease in complexity of the aroma profile, but total area counts increased compared with harvest levels. The quantitative trait loci (QTLs) that induced climacteric behavior in the NILs strongly affected the aroma profile during senescence. In senescent climacteric fruit, the relative contributions of acetate and non-acetate esters to the overall aroma profile increased in relation to harvest levels, particularly propyl acetate, isobutyl acetate, methyl 2-methylbutanoate, ethyl butanoate, hexyl acetate and benzyl acetate. In the senescent non-climacteric NILs, 15-17 volatiles of the profile were undetectable, particularly some aldehydes, while other aldehydes were reduced, esters did not change from harvest to senescence and sulfur-derived compounds increased. Methyl propanoate decreased with senescence irrespective of the climacteric behavior. The climacteric senescent fruit of the NILs could be discriminated from the non-climacteric fruit by 11 compounds of the profile, mainly benzyl acetate, hexanal and pentanal among others (ethyl acetate, propan-2-yl acetate, nonanal, 2-ethylhexan-1-ol, methyldisulfanylmethane, acetone and 2-ethylfuran). The grouping of senescent climacteric NILs was mainly attributable to high relative values of ethyl acetate, methyl butyrate and propyl acetate. Finally, the physiological behavior of NILs also allowed to classify methyl propanoate, ethyl 2-methylpropanoate, pentanal, acetone and 2-ethylfuran as ethylene-independent aroma compounds while isobutyl acetate was classified as ethylene-dependent.

... melo L.) were produced in two seasons (2005 and 2006) containing introgressions of different ... more ... melo L.) were produced in two seasons (2005 and 2006) containing introgressions of different extent from the Korean accession 'Shongwan Charmi' PI161375 (SC ... It is assumed that within the normal time frame of storage, these structural issues do not change upon ripening. ...

Postharvest Biology and Technology, 2009

In two consecutive seasons the firmness of 13-15 near-isogenic lines (NILs) of melons (Cucumis me... more In two consecutive seasons the firmness of 13-15 near-isogenic lines (NILs) of melons (Cucumis melo L.) was followed during storage at 21 • C. Firmness was measured using non-destructive compression of whole melon fruit to a predefined compression distance of 2 mm. The same individuals (about 6 per near-isogenic line) were repeatedly measured over time. Integral statistical analysis of all individuals using non-linear mixed effects regression analysis revealed that the rate constant of the exponential firmness decrease was the same for all NILs irrespective of their differences in introgression in linkage III or in the other two linkage groups. The only difference observed was found in the (asymptotic) end value of softening. That would imply that the process of softening is very similar, although over a different range for each melon. Melons from some NILs were firmer and showed a higher end value of softening than those of other NILs. The percentage variance accounted for (R 2 adj ) was 94% (523 observations) for the 2005 season and 85% (829 observations) for the 2006 season. A small variation in asymptotic end value together with a low end value as to ascertain edibility could be a good indication of the usefulness of certain NILs for commercial application.

Postharvest Biology and Technology, 2008

A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an ... more A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an exotic non-climacteric accession of Cucumis melo 'Shongwan Charmi' [SC (PI 161375), Conomon Group)] into the non-climacteric Spanish Inodorus type of melon cultivar 'Piel de Sapo' ...

Journal of The Science of Food and Agriculture, 2009

BACKGROUND: This paper characterizes the quality traits at harvest and the changes associated wit... more BACKGROUND: This paper characterizes the quality traits at harvest and the changes associated with fruit senescence based on fruit physiological behaviour (climacteric or non-climacteric) found in a collection of near-isogenic lines (NILs) of melon (Cucumis melo L.). Data from both stages of postharvest development were analyzed by univariate and multivariate statistical analysis.RESULTS: The principal components and random forest analyses of the fruit quality traits allowed the best classification of the NILs by time (harvest, senescence), or by climacteric behaviour at harvest, but not at the senescent stage. The overall quality profile of the non-climacteric senescent melons was, in general, very different from that of the climacteric ones, and was in accord with a longer storage life. Most of the taste quality traits (individual sugars or sucrose equivalents, titratable acidity and the citric, oxalacetic, glutamic and succinic acids) and the traits related to skin, flesh and juice colour parameters (chroma, hue angle) helped to distinguish the climacteric NILs from the non-climacteric ones independently of the time considered.CONCLUSIONS: The time had a stronger effect on quality than the physiological behaviour. The discrimination by climacteric or non-climacteric behaviour was usually better at harvest than at the senescent stage irrespective of the methodology used. Principal component analysis was the best multivariate method to discriminate by time and physiological behaviour followed by random forest and linear discriminant analysis. Copyright © 2009 Society of Chemical Industry

Scientia Horticulturae, 2009

Postharvest Biology and Technology, 2008

A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an ... more A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an exotic non-climacteric accession of Cucumis melo 'Shongwan Charmi' [SC (PI 161375), Conomon Group)] into the non-climacteric Spanish Inodorus type of melon cultivar 'Piel de Sapo' ...

Food Chemistry, 2010

... The set of 25 melon near-isogenic lines (NILs) developed by introgressing Korean accession &a... more ... The set of 25 melon near-isogenic lines (NILs) developed by introgressing Korean accession 'Shongwan Charmi' PI 161375 (SC; Group Conomon) genomic regions into 'Piel de sapo' (PS) [C. melo var. inodorus H. Jacq. (Group Inodorus)] genome (Eduardo, Arús, & Monforte ...

Crop Science, 1994

Plant breeders have an interest in the identification of genomic regions associated with the expr... more Plant breeders have an interest in the identification of genomic regions associated with the expression of quantitative traits because they recognize that such information could be used to improve realized heritability and reduce time per cycle of selection. The development of molecular marker technologies may provide tools to accomplish these goals. Maize (Zea mays L.) breeders develop new inbred parents of hybrids through toperossed and per se evaluation of numerous quantitative traits in segregating progeny from planned breeding crosses. This study was conducted to determine if regions of the genome associated with variability of agronomicaily important traits could be identified by a small, but typical, sample of toperossed and F~:4 progeny from the cross B73×MOIT. We identified genomic regions in 24 agronomic traits, using interval mapping and simultaneous estimation of multiple quantitative trait locus models. The estimated numbers of quantitative trait loci (QTL) identified per trait were about three five. Many of the identified QTL were the same for correlated traits. Interestingly, yield QTL were not in the same regions previously reported for B73×MO17. This comparison contains a number of potential confounding factors: source of parental inbreds, type of progeny, different genotype × environment interaction effects, and different small samples of progeny from the cross. Consideration of these factors and the power of the tests to identify QTL suggests that the sampling of progeny is the most likely explanation for the differences.

Around the world, the agroindustrial sector is highly dependent on the fossil fuels for its produ... more Around the world, the agroindustrial sector is highly dependent on the fossil fuels for its production process. Therefore, there is an urgent need to find new alternatives of energy resources in order to replace this dependence and make the process more efficient, support the production process of small and medium-sized farmers with clean technologies and achieve the mitigation of the greenhouse gases to contribute to diminish the effect of the climate change from a local initiative. The aim of this research was to evaluate the implementation of a thermal hybrid forced system (LP gas solar hybrid system) in two agricultural production units for the milk pasteurization to process fresh cheese for the local market. The system was implemented in two milk factories in the Northern Huetar Region of Costa Rica. This area is characterized by its high cloudiness and high volume precipitation, aspects that have limited the use of this alternative technology through the years. The system repl...

Postharvest Biology and Technology, 2009

This paper characterizes the changes in aroma volatiles associated with fruit senescence based on... more This paper characterizes the changes in aroma volatiles associated with fruit senescence based on the climacteric or non-climacteric behavior found in a collection of near-isogenic lines (NILs) of melon (Cucumis melo L.). Data were analyzed by univariate and multivariate methodologies. In general, senescence led to a decrease in complexity of the aroma profile, but total area counts increased compared with harvest levels. The quantitative trait loci (QTLs) that induced climacteric behavior in the NILs strongly affected the aroma profile during senescence. In senescent climacteric fruit, the relative contributions of acetate and non-acetate esters to the overall aroma profile increased in relation to harvest levels, particularly propyl acetate, isobutyl acetate, methyl 2-methylbutanoate, ethyl butanoate, hexyl acetate and benzyl acetate. In the senescent non-climacteric NILs, 15-17 volatiles of the profile were undetectable, particularly some aldehydes, while other aldehydes were reduced, esters did not change from harvest to senescence and sulfur-derived compounds increased. Methyl propanoate decreased with senescence irrespective of the climacteric behavior. The climacteric senescent fruit of the NILs could be discriminated from the non-climacteric fruit by 11 compounds of the profile, mainly benzyl acetate, hexanal and pentanal among others (ethyl acetate, propan-2-yl acetate, nonanal, 2-ethylhexan-1-ol, methyldisulfanylmethane, acetone and 2-ethylfuran). The grouping of senescent climacteric NILs was mainly attributable to high relative values of ethyl acetate, methyl butyrate and propyl acetate. Finally, the physiological behavior of NILs also allowed to classify methyl propanoate, ethyl 2-methylpropanoate, pentanal, acetone and 2-ethylfuran as ethylene-independent aroma compounds while isobutyl acetate was classified as ethylene-dependent.

... melo L.) were produced in two seasons (2005 and 2006) containing introgressions of different ... more ... melo L.) were produced in two seasons (2005 and 2006) containing introgressions of different extent from the Korean accession 'Shongwan Charmi' PI161375 (SC ... It is assumed that within the normal time frame of storage, these structural issues do not change upon ripening. ...

Postharvest Biology and Technology, 2009

In two consecutive seasons the firmness of 13-15 near-isogenic lines (NILs) of melons (Cucumis me... more In two consecutive seasons the firmness of 13-15 near-isogenic lines (NILs) of melons (Cucumis melo L.) was followed during storage at 21 • C. Firmness was measured using non-destructive compression of whole melon fruit to a predefined compression distance of 2 mm. The same individuals (about 6 per near-isogenic line) were repeatedly measured over time. Integral statistical analysis of all individuals using non-linear mixed effects regression analysis revealed that the rate constant of the exponential firmness decrease was the same for all NILs irrespective of their differences in introgression in linkage III or in the other two linkage groups. The only difference observed was found in the (asymptotic) end value of softening. That would imply that the process of softening is very similar, although over a different range for each melon. Melons from some NILs were firmer and showed a higher end value of softening than those of other NILs. The percentage variance accounted for (R 2 adj ) was 94% (523 observations) for the 2005 season and 85% (829 observations) for the 2006 season. A small variation in asymptotic end value together with a low end value as to ascertain edibility could be a good indication of the usefulness of certain NILs for commercial application.

Postharvest Biology and Technology, 2008

A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an ... more A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an exotic non-climacteric accession of Cucumis melo 'Shongwan Charmi' [SC (PI 161375), Conomon Group)] into the non-climacteric Spanish Inodorus type of melon cultivar 'Piel de Sapo' ...

Journal of The Science of Food and Agriculture, 2009

BACKGROUND: This paper characterizes the quality traits at harvest and the changes associated wit... more BACKGROUND: This paper characterizes the quality traits at harvest and the changes associated with fruit senescence based on fruit physiological behaviour (climacteric or non-climacteric) found in a collection of near-isogenic lines (NILs) of melon (Cucumis melo L.). Data from both stages of postharvest development were analyzed by univariate and multivariate statistical analysis.RESULTS: The principal components and random forest analyses of the fruit quality traits allowed the best classification of the NILs by time (harvest, senescence), or by climacteric behaviour at harvest, but not at the senescent stage. The overall quality profile of the non-climacteric senescent melons was, in general, very different from that of the climacteric ones, and was in accord with a longer storage life. Most of the taste quality traits (individual sugars or sucrose equivalents, titratable acidity and the citric, oxalacetic, glutamic and succinic acids) and the traits related to skin, flesh and juice colour parameters (chroma, hue angle) helped to distinguish the climacteric NILs from the non-climacteric ones independently of the time considered.CONCLUSIONS: The time had a stronger effect on quality than the physiological behaviour. The discrimination by climacteric or non-climacteric behaviour was usually better at harvest than at the senescent stage irrespective of the methodology used. Principal component analysis was the best multivariate method to discriminate by time and physiological behaviour followed by random forest and linear discriminant analysis. Copyright © 2009 Society of Chemical Industry

Scientia Horticulturae, 2009

Postharvest Biology and Technology, 2008

A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an ... more A near-isogenic line (NIL) SC3-5 and a further nine NILs of melon contained introgressions of an exotic non-climacteric accession of Cucumis melo 'Shongwan Charmi' [SC (PI 161375), Conomon Group)] into the non-climacteric Spanish Inodorus type of melon cultivar 'Piel de Sapo' ...

Food Chemistry, 2010

... The set of 25 melon near-isogenic lines (NILs) developed by introgressing Korean accession &a... more ... The set of 25 melon near-isogenic lines (NILs) developed by introgressing Korean accession 'Shongwan Charmi' PI 161375 (SC; Group Conomon) genomic regions into 'Piel de sapo' (PS) [C. melo var. inodorus H. Jacq. (Group Inodorus)] genome (Eduardo, Arús, & Monforte ...