Jaya Kumari - Academia.edu (original) (raw)
Uploads
Papers by Jaya Kumari
Aquaculture, 2017
The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manua... more The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applications. The main objective of this study was to develop a convenient, simple immersion delivery model for unfertilized Atlantic salmon (Salmo salar L.) eggs using liposomes as a system for the delivery of selected molecules. We used a lipophilic marker, Rhodamine B isothiocyanate (RBITC) dye, to follow liposomal uptake, marker release and marker distribution within incubated egg. We investigated the influence of the incubation time, liposome surface charges and liposome sizes on the uptake into the eggs. After incubation with liposomes, the exposed eggs were fertilized and embryonic development was monitored until complete yolk sac absorption. The result showed that RBITC, was successfully delivered to the yolk of the incubated eggs. Moreover, during embryonic development, liposomal RBITC remained in the yolk sac until the yolk was completely absorbed. The findings demonstrated a novel approach for the delivery of exogenous molecules to unfertilized Atlantic salmon eggs, opening an avenue for large-scale aquaculture therapeutic applications.
Indian journal of experimental biology, 2007
A series of experiments were performed to determine the impact of polyherbal immunomodulatory for... more A series of experiments were performed to determine the impact of polyherbal immunomodulatory formulation 'ImmuPlus' (AquaImmu) on growth, immunity and disease resistance of rohu (Labeo rohita), one of the Indian major carp at different stages of growth. Rohu larvae were fed on plankton, ImmuPlus-mixed compound feed, and plankton plus ImmuPlus-mixed compound feed (ImmuPlus added at three dose levels of 0.25, 0.50, and 0.75 g/kg feed) from 4th day of hatching to 14th day. ImmuPlus-mixed diets enhanced growth of larvae, survival and disease resistance against Aeromonas hydrophila challenge, compared to only plankton-fed group. In two other experiments, advanced rohu larvae and fingerlings were fed with ImmuPlus-mixed compound feed (at 0, 0.5, 1.0 and 2.0 g/kg) for 15, 30 and 45 days. At the end of 45 days for advanced larvae and 30 days for fingerlings, the fish fed with ImmuPlus at 1.0 g/kg level showed significantly higher growth and disease resistance against A. hydrophila ...
Aquaculture, 2017
The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manua... more The delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applications. The main objective of this study was to develop a convenient, simple immersion delivery model for unfertilized Atlantic salmon (Salmo salar L.) eggs using liposomes as a system for the delivery of selected molecules. We used a lipophilic marker, Rhodamine B isothiocyanate (RBITC) dye, to follow liposomal uptake, marker release and marker distribution within incubated egg. We investigated the influence of the incubation time, liposome surface charges and liposome sizes on the uptake into the eggs. After incubation with liposomes, the exposed eggs were fertilized and embryonic development was monitored until complete yolk sac absorption. The result showed that RBITC, was successfully delivered to the yolk of the incubated eggs. Moreover, during embryonic development, liposomal RBITC remained in the yolk sac until the yolk was completely absorbed. The findings demonstrated a novel approach for the delivery of exogenous molecules to unfertilized Atlantic salmon eggs, opening an avenue for large-scale aquaculture therapeutic applications.
Indian journal of experimental biology, 2007
A series of experiments were performed to determine the impact of polyherbal immunomodulatory for... more A series of experiments were performed to determine the impact of polyherbal immunomodulatory formulation 'ImmuPlus' (AquaImmu) on growth, immunity and disease resistance of rohu (Labeo rohita), one of the Indian major carp at different stages of growth. Rohu larvae were fed on plankton, ImmuPlus-mixed compound feed, and plankton plus ImmuPlus-mixed compound feed (ImmuPlus added at three dose levels of 0.25, 0.50, and 0.75 g/kg feed) from 4th day of hatching to 14th day. ImmuPlus-mixed diets enhanced growth of larvae, survival and disease resistance against Aeromonas hydrophila challenge, compared to only plankton-fed group. In two other experiments, advanced rohu larvae and fingerlings were fed with ImmuPlus-mixed compound feed (at 0, 0.5, 1.0 and 2.0 g/kg) for 15, 30 and 45 days. At the end of 45 days for advanced larvae and 30 days for fingerlings, the fish fed with ImmuPlus at 1.0 g/kg level showed significantly higher growth and disease resistance against A. hydrophila ...