Jayati Mallick - Academia.edu (original) (raw)

Papers by Jayati Mallick

C67. THORACIC ONCOLOGY CASE REPORTS I, 2020

Human Pathology: Case Reports, Sep 1, 2020

Background Extranodal natural killer (NK)/T-cell lymphoma is a subtype of lymphoma that is derive... more Background Extranodal natural killer (NK)/T-cell lymphoma is a subtype of lymphoma that is derived from NK cells. It is considered as an aggressive form of non-Hodgkin's lymphoma because of frequent relapses and resistance to treatment. Relapsed NK/T-cell lymphoma often follows a fulminant course that is refractory to conventional chemotherapy treatment. Methods Several patients with extranodal NK/T-cell lymphoma showed long-term survival in spite of frequent relapses. Thus, the medical records of patients diagnosed with extranodal NK/T-cell lymphoma from 1995 to 2007 were reviewed and assessed. Results Of the 140 cases reviewed, 6 were selected (4.29%). Each of these patients had a minimum of 3 relapses or disease progression during the follow-up period, and their median overall survival was 66 months (range, 42-89 months). They were grouped according to the atypical clinical behavior observed: (1) repeated relapses or progression (≥3 times) during follow-up; and (2) long-term survival of more than 40 months, as the longest overall survival median was previously considered at approximately 40 months. The clinicopathological and laboratory characteristics of these patients were similar to those of other extranodal NK/T-cell lymphoma patients. However, 5 of the studied cases involved relatively lower expression of the proliferation-related antigen Ki-67 (＜40-50%), indicating less proliferative activity. Clinically, they showed delayed relapse for at least 20 months after the initial complete remission. Conclusion Our observations suggest that the clinical behavior of some extranodal NK/T-cell lymphoma patients differs from the typical clinical course.

Frontiers in Oncology

Primary bone lymphoma (PBL) is a rare extranodal presentation within lymphomas and primary bone m... more Primary bone lymphoma (PBL) is a rare extranodal presentation within lymphomas and primary bone malignancies. Pathologic fracture (PF) is a common complication of metastatic bone disease but is, rarely, the presentation of a primary bone tumor. We report a case of an 83-year-old man with a history of untreated prostate cancer, presenting with atraumatic fracture of his left femur after months of intermittent pains and weight loss. Radiographic workup revealed a lytic lesion suspicious for PF secondary to metastatic prostate cancer; however, initial core biopsy results were inconclusive for malignancy. A complete blood count with differential and complete metabolic panel was within normal limits. During surgical fixation and nailing of the femur, a reaming biopsy was performed as a repeat measure and revealed diffuse large B-cell lymphoma. Staging with positron emission tomography and computed tomography found no evidence of lymphatic or visceral involvement and chemotherapy was prom...

eJHaem

Diffuse large B-cell lymphoma (DLBCL) arising in the spleen, also known as primary splenic DLBCL ... more Diffuse large B-cell lymphoma (DLBCL) arising in the spleen, also known as primary splenic DLBCL (PS-DLBCL), is a rare form of malignant lymphoma. It is defined as a lymphoma confined to the spleen or involving splenic hilar lymph nodes. Here we report a case of PS-DLBCL with CD30. The patient was a 62-year-old who presented with 2 weeks of left flank pain, chills, and abdominal distension. Computed tomography identified an 8-cm splenic mass with central necrosis interpreted as an abscess. A drain was placed, yielding purulent necrotic material; cytologically, only neutrophils were identified. However, purulent drainage continued for 28 days without resolution, prompting splenectomy. Pathological dissection revealed a multinodular mass with central necrosis. Microscopic examination revealed extensive karyorrhexis, abundant ghosts of large cells, and scattered large cells with pleomorphic, multilobated, and vesicular nuclei with moderately abundant cytoplasm. Immunohistochemical staining revealed large,

International Journal of Surgical Pathology

Peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS) is a relatively rare mature T-cel... more Peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS) is a relatively rare mature T-cell lymphoma that cannot be categorized under any of the well-defined category. This type of aggressive lymphoma mostly involves the lymph nodes, though any other organ can be affected. Leukemic presentation is extremely rare. No case report of isolated leukemic presentation was found after detail literature search. Herein we present a case of PTCL, NOS with leukemic presentation.

International Journal of Surgical Pathology, Oct 19, 2022

eJHaem

A 74-year-old female was referred for evaluation of axillary lymphadenopathy found on routine scr... more A 74-year-old female was referred for evaluation of axillary lymphadenopathy found on routine screening mammography. Suspicion for lymphoma involvement was raised. The consequent lymph node biopsy showed synchronous involvement by small lymphocytic F I G U R E 1 Core needle biopsy shows lymphoid tissue heavily infiltrated by large, pleomorphic cells with prominent nucleoli in a background of small monomorphous lymphocytes with irregular nuclear borders concerning for lymphoma (top left, H&E). The carcinoma component is positive for CK903 (top center), where lymphoma component is positive for CD23 (top right), CD5 (bottom left), and CD20 (bottom center) and negative for CyclinD1 (bottom right). Interestingly that carcinoma cells show CyclinD1 positivity (bottom right) This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Pediatric Hematology and Oncology

Human Pathology: Case Reports, 2021

Thymoma can cause several autoimmune conditions in a number of patients. Nephrotic syndrome is co... more Thymoma can cause several autoimmune conditions in a number of patients. Nephrotic syndrome is considered to be a rare systemic manifestation from thymic lesions. The most common renal pathology associated with thymoma is minimal change disease (MCD). Herein, we reported a case of 58-year-old man with type B3 thymoma, who developed nephrotic syndrome after the tumor recurrence. The renal biopsy showed the morphologic features of membranous nephropathy (MN). Both immunofluorescent study and serum level for phospholipase A2 receptor (PLA2R) antibody were negative, suggesting a secondary type. Literature review suggests that there is a correlation between thymoma subtype and renal pathology, i.e. lymphocyte predominant thymoma (type B1, B2) is associated with MCD, while type B3 thymoma is associated with MN.

American Journal of Clinical Pathology, 2018

The Journal of Pathology: Clinical Research, 2016

The limited clinical success of anti-HGF/MET drugs can be attributed to the lack of predictive bi... more The limited clinical success of anti-HGF/MET drugs can be attributed to the lack of predictive biomarkers that adequately select patients for treatment. We demonstrate here that quantitative digital imaging of formalin fixed paraffin embedded tissues stained by immunohistochemistry can be used to measure signals from weakly staining antibodies and provides new opportunities to develop assays for detection of MET receptor activity. To establish a biomarker panel of MET activation, we employed seven antibodies measuring protein expression in the HGF/MET pathway in 20 cases and up to 80 cores from 18 human cancer types. The antibodies bind to epitopes in the extra (EC)-and intracellular (IC) domains of MET (MET4 EC , SP44_MET IC , D1C2_MET IC), to MET-pY1234/pY1235, a marker of MET kinase activation, as well as to HGF, pSFK or pMAPK. Expression of HGF was determined in tumour cells (T_HGF) as well as in stroma surrounding cancer (St_HGF). Remarkably, MET4 EC correlated more strongly with pMET (r 5 0.47) than SP44_MET IC (r 5 0.21) or D1C2_MET IC (r 5 0.08) across 18 cancer types. In addition, correlation coefficients of pMET and T_HGF (r 5 0.38) and pMET and pSFK (r 5 0.56) were high. Prediction models of MET activation reveal cancer-type specific differences in performance of MET4 EC , SP44_MET IC and anti-HGF antibodies. Thus, we conclude that assays to predict the response to HGF/MET inhibitors require a cancer-type specific antibody selection and should be developed in those cancer types in which they are employed clinically.

Urologic oncology, Jan 8, 2015

Preclinical urothelial carcinoma models suggest activity of dasatinib, an oral SRC-family kinase ... more Preclinical urothelial carcinoma models suggest activity of dasatinib, an oral SRC-family kinase (SFK) inhibitor. We sought to determine the feasibility and biologic activity of neoadjuvant dasatinib (Neo-D) in patients with muscle-invasive urothelial carcinoma of the bladder (miUCB) preceding radical cystectomy (RC). A prospective multisite phase II trial was conducted. Key eligibility criteria included: resectable miUCB (T2-T4a, N0, M0), and Eastern Cooperative Oncology Group performance status 0 to 1. Patients received oral Neo-D 100mg once daily for 28±7 days followed by RC 8 to 24 hours after the last dose. The primary end point was feasibility, defined as≥60% of patients with miUCB completing therapy without treatment-related dose-limiting toxicity (DLT). Pre- and posttreatment tumor immunohistochemistry of phosphorylated SFK (pSFK), Ki-67, and cleaved caspase (Cas)-3 results were analyzed by paired t test. The study completed full accrual with enrollment of 25 patients of who...

Endocrine Abstracts, 2015

Journal of the Indian Medical Association, 2011

Pseudothrombocytopenia is an ex vivo thrombocytopenia, counted by an automated cell counter, but ... more Pseudothrombocytopenia is an ex vivo thrombocytopenia, counted by an automated cell counter, but not configured by microscopic examination of a well prepared blood smear. Incidences of pseudothrombocytopenia reported in different studies range from 0.09-0.21%, which could account for 15-30% of all cases of thrombocytopenla. Failure to identify pseudothrombocytopenia has led to a lot of clinical problems like unnecessary platelet transfusion, glucocorticoid therapy and so on. Haematological cell counters count platelets as particles with volume between 2 and 20fl. Thus platelet clumps are counted as leucocytes while fragmented RBCs as platelets. This study found pseudothrombocytopenia due to causes like EDTA blood stored at less than 32 degrees C for more than 2 hours (35%), excess EDTA in sample (10.7%), hyperlipidaemia, auto-immune disease, pregnancy, etc. No apparent cause could be found in 52% of cases. Hence it is emphasised that direct microscopic examination of a well stained ...

Journal of Cytology, 2012

Hemangioendothelioma is a rare vascular tumor of intermediate malignancy. Cytologically, it can s... more Hemangioendothelioma is a rare vascular tumor of intermediate malignancy. Cytologically, it can simulate a non-vascular malignant tumor. We report two cases of this tumor, which were misdiagnosed at cytology. In the first case, a 27-year-old man presented with an anterior abdominal wall tumor. Fine needle aspiration cytology (FNAC) of the tumor showed polygonal cells with vacuolated cytoplasm in clusters having moderate nuclear atypia in a background of necrosis. A diagnosis of metastatic carcinoma was made. The histological examination showed features of epithelioid hemangioendothelioma. In the second case, a 13-year-old female child presented with unilateral enlargement of the right tonsil. At ultrasound-guided FNAC, a diagnosis of, 'small round cell tumor, could be consistent with alveolar rhabdomyosarcoma,' was made. The histological examination showed features of papillary intralymphatic angioendothelioma (Dabska's tumor). We conclude that epithelioid hemangioendothelioma should be considered in the differential diagnosis of metastatic carcinoma and small round cell tumor even at unusual sites.

Anales de Medicina Interna, 2006

Leukemia Research Reports

C67. THORACIC ONCOLOGY CASE REPORTS I, 2020

Human Pathology: Case Reports, Sep 1, 2020

Background Extranodal natural killer (NK)/T-cell lymphoma is a subtype of lymphoma that is derive... more Background Extranodal natural killer (NK)/T-cell lymphoma is a subtype of lymphoma that is derived from NK cells. It is considered as an aggressive form of non-Hodgkin's lymphoma because of frequent relapses and resistance to treatment. Relapsed NK/T-cell lymphoma often follows a fulminant course that is refractory to conventional chemotherapy treatment. Methods Several patients with extranodal NK/T-cell lymphoma showed long-term survival in spite of frequent relapses. Thus, the medical records of patients diagnosed with extranodal NK/T-cell lymphoma from 1995 to 2007 were reviewed and assessed. Results Of the 140 cases reviewed, 6 were selected (4.29%). Each of these patients had a minimum of 3 relapses or disease progression during the follow-up period, and their median overall survival was 66 months (range, 42-89 months). They were grouped according to the atypical clinical behavior observed: (1) repeated relapses or progression (≥3 times) during follow-up; and (2) long-term survival of more than 40 months, as the longest overall survival median was previously considered at approximately 40 months. The clinicopathological and laboratory characteristics of these patients were similar to those of other extranodal NK/T-cell lymphoma patients. However, 5 of the studied cases involved relatively lower expression of the proliferation-related antigen Ki-67 (＜40-50%), indicating less proliferative activity. Clinically, they showed delayed relapse for at least 20 months after the initial complete remission. Conclusion Our observations suggest that the clinical behavior of some extranodal NK/T-cell lymphoma patients differs from the typical clinical course.

Frontiers in Oncology

Primary bone lymphoma (PBL) is a rare extranodal presentation within lymphomas and primary bone m... more Primary bone lymphoma (PBL) is a rare extranodal presentation within lymphomas and primary bone malignancies. Pathologic fracture (PF) is a common complication of metastatic bone disease but is, rarely, the presentation of a primary bone tumor. We report a case of an 83-year-old man with a history of untreated prostate cancer, presenting with atraumatic fracture of his left femur after months of intermittent pains and weight loss. Radiographic workup revealed a lytic lesion suspicious for PF secondary to metastatic prostate cancer; however, initial core biopsy results were inconclusive for malignancy. A complete blood count with differential and complete metabolic panel was within normal limits. During surgical fixation and nailing of the femur, a reaming biopsy was performed as a repeat measure and revealed diffuse large B-cell lymphoma. Staging with positron emission tomography and computed tomography found no evidence of lymphatic or visceral involvement and chemotherapy was prom...

eJHaem

Diffuse large B-cell lymphoma (DLBCL) arising in the spleen, also known as primary splenic DLBCL ... more Diffuse large B-cell lymphoma (DLBCL) arising in the spleen, also known as primary splenic DLBCL (PS-DLBCL), is a rare form of malignant lymphoma. It is defined as a lymphoma confined to the spleen or involving splenic hilar lymph nodes. Here we report a case of PS-DLBCL with CD30. The patient was a 62-year-old who presented with 2 weeks of left flank pain, chills, and abdominal distension. Computed tomography identified an 8-cm splenic mass with central necrosis interpreted as an abscess. A drain was placed, yielding purulent necrotic material; cytologically, only neutrophils were identified. However, purulent drainage continued for 28 days without resolution, prompting splenectomy. Pathological dissection revealed a multinodular mass with central necrosis. Microscopic examination revealed extensive karyorrhexis, abundant ghosts of large cells, and scattered large cells with pleomorphic, multilobated, and vesicular nuclei with moderately abundant cytoplasm. Immunohistochemical staining revealed large,

International Journal of Surgical Pathology

Peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS) is a relatively rare mature T-cel... more Peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS) is a relatively rare mature T-cell lymphoma that cannot be categorized under any of the well-defined category. This type of aggressive lymphoma mostly involves the lymph nodes, though any other organ can be affected. Leukemic presentation is extremely rare. No case report of isolated leukemic presentation was found after detail literature search. Herein we present a case of PTCL, NOS with leukemic presentation.

International Journal of Surgical Pathology, Oct 19, 2022

eJHaem

A 74-year-old female was referred for evaluation of axillary lymphadenopathy found on routine scr... more A 74-year-old female was referred for evaluation of axillary lymphadenopathy found on routine screening mammography. Suspicion for lymphoma involvement was raised. The consequent lymph node biopsy showed synchronous involvement by small lymphocytic F I G U R E 1 Core needle biopsy shows lymphoid tissue heavily infiltrated by large, pleomorphic cells with prominent nucleoli in a background of small monomorphous lymphocytes with irregular nuclear borders concerning for lymphoma (top left, H&E). The carcinoma component is positive for CK903 (top center), where lymphoma component is positive for CD23 (top right), CD5 (bottom left), and CD20 (bottom center) and negative for CyclinD1 (bottom right). Interestingly that carcinoma cells show CyclinD1 positivity (bottom right) This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Pediatric Hematology and Oncology

Human Pathology: Case Reports, 2021

Thymoma can cause several autoimmune conditions in a number of patients. Nephrotic syndrome is co... more Thymoma can cause several autoimmune conditions in a number of patients. Nephrotic syndrome is considered to be a rare systemic manifestation from thymic lesions. The most common renal pathology associated with thymoma is minimal change disease (MCD). Herein, we reported a case of 58-year-old man with type B3 thymoma, who developed nephrotic syndrome after the tumor recurrence. The renal biopsy showed the morphologic features of membranous nephropathy (MN). Both immunofluorescent study and serum level for phospholipase A2 receptor (PLA2R) antibody were negative, suggesting a secondary type. Literature review suggests that there is a correlation between thymoma subtype and renal pathology, i.e. lymphocyte predominant thymoma (type B1, B2) is associated with MCD, while type B3 thymoma is associated with MN.

American Journal of Clinical Pathology, 2018

The Journal of Pathology: Clinical Research, 2016

The limited clinical success of anti-HGF/MET drugs can be attributed to the lack of predictive bi... more The limited clinical success of anti-HGF/MET drugs can be attributed to the lack of predictive biomarkers that adequately select patients for treatment. We demonstrate here that quantitative digital imaging of formalin fixed paraffin embedded tissues stained by immunohistochemistry can be used to measure signals from weakly staining antibodies and provides new opportunities to develop assays for detection of MET receptor activity. To establish a biomarker panel of MET activation, we employed seven antibodies measuring protein expression in the HGF/MET pathway in 20 cases and up to 80 cores from 18 human cancer types. The antibodies bind to epitopes in the extra (EC)-and intracellular (IC) domains of MET (MET4 EC , SP44_MET IC , D1C2_MET IC), to MET-pY1234/pY1235, a marker of MET kinase activation, as well as to HGF, pSFK or pMAPK. Expression of HGF was determined in tumour cells (T_HGF) as well as in stroma surrounding cancer (St_HGF). Remarkably, MET4 EC correlated more strongly with pMET (r 5 0.47) than SP44_MET IC (r 5 0.21) or D1C2_MET IC (r 5 0.08) across 18 cancer types. In addition, correlation coefficients of pMET and T_HGF (r 5 0.38) and pMET and pSFK (r 5 0.56) were high. Prediction models of MET activation reveal cancer-type specific differences in performance of MET4 EC , SP44_MET IC and anti-HGF antibodies. Thus, we conclude that assays to predict the response to HGF/MET inhibitors require a cancer-type specific antibody selection and should be developed in those cancer types in which they are employed clinically.

Urologic oncology, Jan 8, 2015

Preclinical urothelial carcinoma models suggest activity of dasatinib, an oral SRC-family kinase ... more Preclinical urothelial carcinoma models suggest activity of dasatinib, an oral SRC-family kinase (SFK) inhibitor. We sought to determine the feasibility and biologic activity of neoadjuvant dasatinib (Neo-D) in patients with muscle-invasive urothelial carcinoma of the bladder (miUCB) preceding radical cystectomy (RC). A prospective multisite phase II trial was conducted. Key eligibility criteria included: resectable miUCB (T2-T4a, N0, M0), and Eastern Cooperative Oncology Group performance status 0 to 1. Patients received oral Neo-D 100mg once daily for 28±7 days followed by RC 8 to 24 hours after the last dose. The primary end point was feasibility, defined as≥60% of patients with miUCB completing therapy without treatment-related dose-limiting toxicity (DLT). Pre- and posttreatment tumor immunohistochemistry of phosphorylated SFK (pSFK), Ki-67, and cleaved caspase (Cas)-3 results were analyzed by paired t test. The study completed full accrual with enrollment of 25 patients of who...

Endocrine Abstracts, 2015

Journal of the Indian Medical Association, 2011

Pseudothrombocytopenia is an ex vivo thrombocytopenia, counted by an automated cell counter, but ... more Pseudothrombocytopenia is an ex vivo thrombocytopenia, counted by an automated cell counter, but not configured by microscopic examination of a well prepared blood smear. Incidences of pseudothrombocytopenia reported in different studies range from 0.09-0.21%, which could account for 15-30% of all cases of thrombocytopenla. Failure to identify pseudothrombocytopenia has led to a lot of clinical problems like unnecessary platelet transfusion, glucocorticoid therapy and so on. Haematological cell counters count platelets as particles with volume between 2 and 20fl. Thus platelet clumps are counted as leucocytes while fragmented RBCs as platelets. This study found pseudothrombocytopenia due to causes like EDTA blood stored at less than 32 degrees C for more than 2 hours (35%), excess EDTA in sample (10.7%), hyperlipidaemia, auto-immune disease, pregnancy, etc. No apparent cause could be found in 52% of cases. Hence it is emphasised that direct microscopic examination of a well stained ...

Journal of Cytology, 2012

Hemangioendothelioma is a rare vascular tumor of intermediate malignancy. Cytologically, it can s... more Hemangioendothelioma is a rare vascular tumor of intermediate malignancy. Cytologically, it can simulate a non-vascular malignant tumor. We report two cases of this tumor, which were misdiagnosed at cytology. In the first case, a 27-year-old man presented with an anterior abdominal wall tumor. Fine needle aspiration cytology (FNAC) of the tumor showed polygonal cells with vacuolated cytoplasm in clusters having moderate nuclear atypia in a background of necrosis. A diagnosis of metastatic carcinoma was made. The histological examination showed features of epithelioid hemangioendothelioma. In the second case, a 13-year-old female child presented with unilateral enlargement of the right tonsil. At ultrasound-guided FNAC, a diagnosis of, 'small round cell tumor, could be consistent with alveolar rhabdomyosarcoma,' was made. The histological examination showed features of papillary intralymphatic angioendothelioma (Dabska's tumor). We conclude that epithelioid hemangioendothelioma should be considered in the differential diagnosis of metastatic carcinoma and small round cell tumor even at unusual sites.

Anales de Medicina Interna, 2006

Leukemia Research Reports