Jhonatas Barbosa - Academia.edu (original) (raw)

Papers by Jhonatas Barbosa

Nanotechnology in the Life Sciences, 2020

The Amazon is an ecological system that comprises a considerable part of South America and that p... more The Amazon is an ecological system that comprises a considerable part of South America and that presents a great biodiversity of plants and fruits. Most of them have bioactive compounds, such as polyphenols and carotenoids, that are associated with the reduced risk of development of various chronic and degenerative diseases. To obtain such substances, the supercritical fluid extraction stands out among other extraction methods due to environmental concerns, since this technology uses green solvents such as carbon dioxide (CO2), water (H2O), and ethanol (EtOH). In this way, the objective of this work was to present the main solvents used in pressurized solvent extraction, as well as the main bioactive compounds obtained in such processes, and their consequent biological applications.

Porous Polymer Science and Applications, 2022

Porous Polymer Science and Applications, 2022

Avaliação de Impactos e de Sustentabilidade das Atividades Agroambientais, 2019

Nanobiotechnology in Neurodegenerative Diseases, 2019

Metallic nanoparticles biosynthesis studies such as gold, silver, selenium, iron, metal oxides, a... more Metallic nanoparticles biosynthesis studies such as gold, silver, selenium, iron, metal oxides, and others, by microorganisms, especially fungi, have received great interest from the international scientific community because it is an ecological alternative. This chapter aims to evaluate recent advances in biosynthesis of fungal nanoparticles, with emphasis on physicochemical properties, bioactivity, and applications. Recent advances in biotechnology have provided interesting tools for the controlled synthesis of nanoparticles, using fungi as biotechnological factories. Due to the great diversity of polysaccharide enzymes and polysaccharide structures synthesized by fungi, it makes this kingdom very promising for the nanoparticles biosynthesis. The synthesized nanoparticles present different physicochemical properties, as they depend on biochemical parameters related to the biosynthesis process, besides the conditions used during the process, such as temperature, pH and substrate concentration. Metal nanoparticles associated with polysaccharides and proteins; depict bioactivity related to treatment of diseases such as neurodegenerative diseases. These nanoparticles are ecologically friendly and hence can be applied in medicine.

A Preservação do Meio Ambiente e o Desenvolvimento Sustentável, 2019

Carboxymethylcellulose (CMC) is obtained by the carboxymethylation of cellulose, which modifies i... more Carboxymethylcellulose (CMC) is obtained by the carboxymethylation of cellulose, which modifies its structure and results in excellent ability to form biodegradable low-toxicity films. In addition, CMC has strong hydrophilicity and a stable internal network structure. Such properties have been widely used to produce composite films with improved performance. Lipid compounds are often used to form moisture barrier films/coatings. For example, lipid compounds coupled with a fruit and vegetable coating are able to reduce desiccation-induced weight loss during storage by 40%–75%. The confinement and interactions between polymers and essential oils (EOs) greatly affect the dynamics and static of biocomposite polymers. EOs cause interactions that can “attract” or “repel” the polymeric matrix, and this ends up creating peaks with different properties depending on the concentration of EO added. The antimicrobial properties of EOs derived from many plants have been widely recognized for cent...

Recent Advances in Microbial Degradation, 2021

Aflatoxins are low-molecular-weight lipophilic compounds widely distributed in nature. Long-term ... more Aflatoxins are low-molecular-weight lipophilic compounds widely distributed in nature. Long-term aflatoxin exposure can lead to several consequences, including carcinogenic effect and it can affect all body systems, causing immune system dysfunction and impaired growth in humans. The literature describes 20 types of aflatoxins. Diseases caused by the consumption of aflatoxin are known as aflatoxicosis. Seeds that suffer stress, such as prolonged drought, short irrigation time, damage caused by insects, and use of seeds in areas that do not favor their cultivation, have high rates of contamination by fungi that produce aflatoxins, such as Fusarium and Aspergillus. Post-harvest contamination occurs worldwide where there are environmental conditions for the proliferation of aflatoxicogenic fungi, such as inadequate storage, improper drying of grains, and plant species not adapted to the climate. An alternative that has been growing in the recent years is the use of microorganisms for d...

The Journal of Supercritical Fluids, 2022

Contamination and degradation of food products by microorganisms are a major problem for the food... more Contamination and degradation of food products by microorganisms are a major problem for the food industry, with serious damage to economic circulation and public health worldwide. Therefore, the objective of this chapter is to clarify how the contamination of microorganisms occurs, the main damage to food, production of toxic substances, economic losses, and new food preservation technologies, with a focus on developing a sustainable and safe food industry. Most foods contain structural ingredients, such as a high concentration of carbohydrates, perfect conditions for an outbreak of microbial infection. The microorganisms that grow in food alter their chemical composition, appearance, taste, texture, and characteristics such as color, shape, and aroma. In addition, some fungi produce mycotoxins that are highly toxic. Finally, contaminated food undergoes degradation and putrefaction, causing irreparable losses to producers. Thus, we address new technologies that may be the key to in...

Biochemistry and Health Benefits of Fatty Acids, 2018

Inovação em Ciência e Tecnologia de Alimentos 2, 2019

Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atrib... more Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

Trends in Food Science & Technology, 2021

Background The global crisis caused by the outbreak of severe acute respiratory syndrome caused b... more Background The global crisis caused by the outbreak of severe acute respiratory syndrome caused by the SARS-CoV-2 virus, better known as COVID-19, brought the need to improve the population's immunity. The foods rich in polysaccharides with immunomodulation properties are among the most highly rated to be used as immune response modulators. Thus, the use of polysaccharides obtained from food offers an innovative strategy to prevent serious side effects of viral infections. Scope and approach This review revisits the current studies on the pathophysiology of SARS-CoV-2, its characteristics, target cell interactions, and the possibility of using polysaccharides from functional foods as activators of the immune response. Several natural foods are explored for the possibility of being used to obtain polysaccharides with immunomodulatory potential. And finally, we address expectations for the use of polysaccharides in the development of potential therapies and vaccines. Key findings and conclusions The negative consequences of the SARS-CoV-2 pandemic across the world are unprecedented, thousands of lives lost, increasing inequalities, and incalculable economic losses. On the other hand, great scientific advances have been made regarding the understanding of the disease and forms of treatment. Polysaccharides, due to their characteristics, have the potential to be used as potential drugs with the ability to modulate the immune response. In addition, they can be used safely, as they have no toxic effects, are biocompatible and biodegradable. Finally, these biopolymers can still be used in the development of new therapies and vaccines.

Resources, Conservation and Recycling, 2021

Abstract Demand for food production has been promoting an increase in the generation of agro-indu... more Abstract Demand for food production has been promoting an increase in the generation of agro-industrial wastes. Although in the past these wastes were mainly seen as a big issue, in the current scenario they are seen as the key strategy for the development of sustainable industrial processes. In this context, several new environmentally friendly technologies such as supercritical fluid extraction (SFE), microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) have been studied and applied to obtain products with high added value in order to supply the chemical, pharmaceutical and food industries. Similarly, the energy generation from biomass and production of new biomaterials such as bionanofilms, bioaerogels, hydrogels and nanocomposites have been intensely studied in order to cross the barriers of the laboratory scale. Therefore, this review aims to update the scientific community, industries, governments and society on the sustainable applications, latest advances and trends in the valorization of agro-industrial wastes for new product development, highlighting the new possibilities for the progress of sustainable production.

Green Sustainable Process for Chemical and Environmental Engineering and Science, 2021

Abstract The production of biosurfactant has undergone significant changes, especially when assoc... more Abstract The production of biosurfactant has undergone significant changes, especially when associated with alternatives for more sustainable and ecologically correct production. In this context, the use of bioreactors has undergone several changes over the past few years. Three different methods, based on the types of fermentation bioreactor, were implemented and evaluated for the optimization of the production capacity of biosurfactants. During the production of biosurfactants, several parameters directly influence quality and yield, including the type of bioreactor and process parameters such as temperature, agitation, type of microbial strain, and others. Each type of bioreactor provides advantages and disadvantages, and the application of microbial strains implies a series of new challenges for the efficient production of biosurfactant. Finally, the future of the biosurfactant industry depends on advances in the biotechnology of new, more productive microbial strains and the development of processes with more efficient and economical bioreactors.

Carbohydrate Polymers, 2020

Food Chemistry, 2020

Pleurotus ostreatus is an edible mushroom with pharmacological potential, due to its metabolites,... more Pleurotus ostreatus is an edible mushroom with pharmacological potential, due to its metabolites, mainly polysaccharides. On here, the development of a new methodology for the recovery of extract rich in antioxidant polysaccharide was reported. The extracts were characterized, evaluated for antioxidant activity in vitro and in cell models and cytotoxicity. The best defined extraction condition was 25 MPa, 433.15 K, and 20% H2O, with 30.69% of the total yield and 0.921 mg of CHO3. The anomeric bonds, identified in the FTIR and NMR spectrum, indicate that the extracts are a mixture of heteropolysaccharides, β-glucans, α-glucans, and oligosaccharides. The best extraction condition has 80.83% of antioxidant activity, without cytotoxic effect in vitro. In addition to antioxidant activity in cell model, increasing protection against oxidative damage induced by H2O2. Finally, H2O + CO2-SFE technology can be used to obtain extracts rich in antioxidant polysaccharides with pharmacological and food potential.

Carbohydrate Polymers, 2019

The biodiversity of mushrooms Pleurotus spp. is impressive due to its complexity and diversity re... more The biodiversity of mushrooms Pleurotus spp. is impressive due to its complexity and diversity related to the composition of chemical structures such as polysaccharides, glycoproteins and secondary metabolites such as alkaloids, flavonoids and betalains. Recent studies of polysaccharides and their structural elucidation have helped to direct research and development of technologies related to pharmacological action, production of bioactive foods and application of new, more sophisticated extraction tools. The diversity of bioactivities related to these biopolymers, their mechanisms and routes of action are constant focus of researches. The elucidation of bioactivities has helped to formulate new vaccines and targeted drugs. In this context, in terms of polysaccharides and the diversity of mushrooms Pleurotus spp., this review seeks to revisit the genus, making an updated approach on the recent discoveries of polysaccharides, new extraction techniques and bioactivities, emphasising on their mechanisms and routes in order to update the reader on the recent technologies related to these polymers.

Carbohydrate Polymers, 2019

The aim of this study was to evaluate and characterize the interaction between fish gelatin (FG) ... more The aim of this study was to evaluate and characterize the interaction between fish gelatin (FG) and Gum Arabic(GA) and its effects in obtaining optimal atomization conditions. The optimal conditions (D = 0.866) founded in this paper were: Gum Arabic concentration of 33.4% and inlet air temperature of 130 °C. These conditions afforded 6.62 g/h yield, 0.27 aw and 247 g of Gel Strength, that are considered as suitable characteristics for food grade gelatin. The complex formed (FG-GA) was successfully obtained, as demonstrated by the results of amino acid profile, SDS-PAGE, FTIR spectroscopy, zeta potential and morphology. It was also verified that the formation of FG-GA promotes positive changes, such as higher atomization yield, adequate Gel Strength, low hygroscopicity and high solubility. The technological properties of FG-GA shown high potential to be applied in the food industry as well in other industrial fields like chemical and pharmaceutical areas.

Nanotechnology in the Life Sciences, 2020

The Amazon is an ecological system that comprises a considerable part of South America and that p... more The Amazon is an ecological system that comprises a considerable part of South America and that presents a great biodiversity of plants and fruits. Most of them have bioactive compounds, such as polyphenols and carotenoids, that are associated with the reduced risk of development of various chronic and degenerative diseases. To obtain such substances, the supercritical fluid extraction stands out among other extraction methods due to environmental concerns, since this technology uses green solvents such as carbon dioxide (CO2), water (H2O), and ethanol (EtOH). In this way, the objective of this work was to present the main solvents used in pressurized solvent extraction, as well as the main bioactive compounds obtained in such processes, and their consequent biological applications.

Porous Polymer Science and Applications, 2022

Porous Polymer Science and Applications, 2022

Avaliação de Impactos e de Sustentabilidade das Atividades Agroambientais, 2019

Nanobiotechnology in Neurodegenerative Diseases, 2019

Metallic nanoparticles biosynthesis studies such as gold, silver, selenium, iron, metal oxides, a... more Metallic nanoparticles biosynthesis studies such as gold, silver, selenium, iron, metal oxides, and others, by microorganisms, especially fungi, have received great interest from the international scientific community because it is an ecological alternative. This chapter aims to evaluate recent advances in biosynthesis of fungal nanoparticles, with emphasis on physicochemical properties, bioactivity, and applications. Recent advances in biotechnology have provided interesting tools for the controlled synthesis of nanoparticles, using fungi as biotechnological factories. Due to the great diversity of polysaccharide enzymes and polysaccharide structures synthesized by fungi, it makes this kingdom very promising for the nanoparticles biosynthesis. The synthesized nanoparticles present different physicochemical properties, as they depend on biochemical parameters related to the biosynthesis process, besides the conditions used during the process, such as temperature, pH and substrate concentration. Metal nanoparticles associated with polysaccharides and proteins; depict bioactivity related to treatment of diseases such as neurodegenerative diseases. These nanoparticles are ecologically friendly and hence can be applied in medicine.

A Preservação do Meio Ambiente e o Desenvolvimento Sustentável, 2019

Carboxymethylcellulose (CMC) is obtained by the carboxymethylation of cellulose, which modifies i... more Carboxymethylcellulose (CMC) is obtained by the carboxymethylation of cellulose, which modifies its structure and results in excellent ability to form biodegradable low-toxicity films. In addition, CMC has strong hydrophilicity and a stable internal network structure. Such properties have been widely used to produce composite films with improved performance. Lipid compounds are often used to form moisture barrier films/coatings. For example, lipid compounds coupled with a fruit and vegetable coating are able to reduce desiccation-induced weight loss during storage by 40%–75%. The confinement and interactions between polymers and essential oils (EOs) greatly affect the dynamics and static of biocomposite polymers. EOs cause interactions that can “attract” or “repel” the polymeric matrix, and this ends up creating peaks with different properties depending on the concentration of EO added. The antimicrobial properties of EOs derived from many plants have been widely recognized for cent...

Recent Advances in Microbial Degradation, 2021

Aflatoxins are low-molecular-weight lipophilic compounds widely distributed in nature. Long-term ... more Aflatoxins are low-molecular-weight lipophilic compounds widely distributed in nature. Long-term aflatoxin exposure can lead to several consequences, including carcinogenic effect and it can affect all body systems, causing immune system dysfunction and impaired growth in humans. The literature describes 20 types of aflatoxins. Diseases caused by the consumption of aflatoxin are known as aflatoxicosis. Seeds that suffer stress, such as prolonged drought, short irrigation time, damage caused by insects, and use of seeds in areas that do not favor their cultivation, have high rates of contamination by fungi that produce aflatoxins, such as Fusarium and Aspergillus. Post-harvest contamination occurs worldwide where there are environmental conditions for the proliferation of aflatoxicogenic fungi, such as inadequate storage, improper drying of grains, and plant species not adapted to the climate. An alternative that has been growing in the recent years is the use of microorganisms for d...

The Journal of Supercritical Fluids, 2022

Contamination and degradation of food products by microorganisms are a major problem for the food... more Contamination and degradation of food products by microorganisms are a major problem for the food industry, with serious damage to economic circulation and public health worldwide. Therefore, the objective of this chapter is to clarify how the contamination of microorganisms occurs, the main damage to food, production of toxic substances, economic losses, and new food preservation technologies, with a focus on developing a sustainable and safe food industry. Most foods contain structural ingredients, such as a high concentration of carbohydrates, perfect conditions for an outbreak of microbial infection. The microorganisms that grow in food alter their chemical composition, appearance, taste, texture, and characteristics such as color, shape, and aroma. In addition, some fungi produce mycotoxins that are highly toxic. Finally, contaminated food undergoes degradation and putrefaction, causing irreparable losses to producers. Thus, we address new technologies that may be the key to in...

Biochemistry and Health Benefits of Fatty Acids, 2018

Inovação em Ciência e Tecnologia de Alimentos 2, 2019

Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atrib... more Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

Trends in Food Science & Technology, 2021

Background The global crisis caused by the outbreak of severe acute respiratory syndrome caused b... more Background The global crisis caused by the outbreak of severe acute respiratory syndrome caused by the SARS-CoV-2 virus, better known as COVID-19, brought the need to improve the population's immunity. The foods rich in polysaccharides with immunomodulation properties are among the most highly rated to be used as immune response modulators. Thus, the use of polysaccharides obtained from food offers an innovative strategy to prevent serious side effects of viral infections. Scope and approach This review revisits the current studies on the pathophysiology of SARS-CoV-2, its characteristics, target cell interactions, and the possibility of using polysaccharides from functional foods as activators of the immune response. Several natural foods are explored for the possibility of being used to obtain polysaccharides with immunomodulatory potential. And finally, we address expectations for the use of polysaccharides in the development of potential therapies and vaccines. Key findings and conclusions The negative consequences of the SARS-CoV-2 pandemic across the world are unprecedented, thousands of lives lost, increasing inequalities, and incalculable economic losses. On the other hand, great scientific advances have been made regarding the understanding of the disease and forms of treatment. Polysaccharides, due to their characteristics, have the potential to be used as potential drugs with the ability to modulate the immune response. In addition, they can be used safely, as they have no toxic effects, are biocompatible and biodegradable. Finally, these biopolymers can still be used in the development of new therapies and vaccines.

Resources, Conservation and Recycling, 2021

Abstract Demand for food production has been promoting an increase in the generation of agro-indu... more Abstract Demand for food production has been promoting an increase in the generation of agro-industrial wastes. Although in the past these wastes were mainly seen as a big issue, in the current scenario they are seen as the key strategy for the development of sustainable industrial processes. In this context, several new environmentally friendly technologies such as supercritical fluid extraction (SFE), microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) have been studied and applied to obtain products with high added value in order to supply the chemical, pharmaceutical and food industries. Similarly, the energy generation from biomass and production of new biomaterials such as bionanofilms, bioaerogels, hydrogels and nanocomposites have been intensely studied in order to cross the barriers of the laboratory scale. Therefore, this review aims to update the scientific community, industries, governments and society on the sustainable applications, latest advances and trends in the valorization of agro-industrial wastes for new product development, highlighting the new possibilities for the progress of sustainable production.

Green Sustainable Process for Chemical and Environmental Engineering and Science, 2021

Abstract The production of biosurfactant has undergone significant changes, especially when assoc... more Abstract The production of biosurfactant has undergone significant changes, especially when associated with alternatives for more sustainable and ecologically correct production. In this context, the use of bioreactors has undergone several changes over the past few years. Three different methods, based on the types of fermentation bioreactor, were implemented and evaluated for the optimization of the production capacity of biosurfactants. During the production of biosurfactants, several parameters directly influence quality and yield, including the type of bioreactor and process parameters such as temperature, agitation, type of microbial strain, and others. Each type of bioreactor provides advantages and disadvantages, and the application of microbial strains implies a series of new challenges for the efficient production of biosurfactant. Finally, the future of the biosurfactant industry depends on advances in the biotechnology of new, more productive microbial strains and the development of processes with more efficient and economical bioreactors.

Carbohydrate Polymers, 2020

Food Chemistry, 2020

Pleurotus ostreatus is an edible mushroom with pharmacological potential, due to its metabolites,... more Pleurotus ostreatus is an edible mushroom with pharmacological potential, due to its metabolites, mainly polysaccharides. On here, the development of a new methodology for the recovery of extract rich in antioxidant polysaccharide was reported. The extracts were characterized, evaluated for antioxidant activity in vitro and in cell models and cytotoxicity. The best defined extraction condition was 25 MPa, 433.15 K, and 20% H2O, with 30.69% of the total yield and 0.921 mg of CHO3. The anomeric bonds, identified in the FTIR and NMR spectrum, indicate that the extracts are a mixture of heteropolysaccharides, β-glucans, α-glucans, and oligosaccharides. The best extraction condition has 80.83% of antioxidant activity, without cytotoxic effect in vitro. In addition to antioxidant activity in cell model, increasing protection against oxidative damage induced by H2O2. Finally, H2O + CO2-SFE technology can be used to obtain extracts rich in antioxidant polysaccharides with pharmacological and food potential.

Carbohydrate Polymers, 2019

The biodiversity of mushrooms Pleurotus spp. is impressive due to its complexity and diversity re... more The biodiversity of mushrooms Pleurotus spp. is impressive due to its complexity and diversity related to the composition of chemical structures such as polysaccharides, glycoproteins and secondary metabolites such as alkaloids, flavonoids and betalains. Recent studies of polysaccharides and their structural elucidation have helped to direct research and development of technologies related to pharmacological action, production of bioactive foods and application of new, more sophisticated extraction tools. The diversity of bioactivities related to these biopolymers, their mechanisms and routes of action are constant focus of researches. The elucidation of bioactivities has helped to formulate new vaccines and targeted drugs. In this context, in terms of polysaccharides and the diversity of mushrooms Pleurotus spp., this review seeks to revisit the genus, making an updated approach on the recent discoveries of polysaccharides, new extraction techniques and bioactivities, emphasising on their mechanisms and routes in order to update the reader on the recent technologies related to these polymers.

Carbohydrate Polymers, 2019

The aim of this study was to evaluate and characterize the interaction between fish gelatin (FG) ... more The aim of this study was to evaluate and characterize the interaction between fish gelatin (FG) and Gum Arabic(GA) and its effects in obtaining optimal atomization conditions. The optimal conditions (D = 0.866) founded in this paper were: Gum Arabic concentration of 33.4% and inlet air temperature of 130 °C. These conditions afforded 6.62 g/h yield, 0.27 aw and 247 g of Gel Strength, that are considered as suitable characteristics for food grade gelatin. The complex formed (FG-GA) was successfully obtained, as demonstrated by the results of amino acid profile, SDS-PAGE, FTIR spectroscopy, zeta potential and morphology. It was also verified that the formation of FG-GA promotes positive changes, such as higher atomization yield, adequate Gel Strength, low hygroscopicity and high solubility. The technological properties of FG-GA shown high potential to be applied in the food industry as well in other industrial fields like chemical and pharmaceutical areas.