Jorge Filho - Academia.edu (original) (raw)

Papers by Jorge Filho

CEP

... CHENNI et al., 2007; KWON; APOSTOLIDIS; SHETTY, 2008; KWON; VATTEN; SHETTY, 2006; MANCINI-FIL... more ... CHENNI et al., 2007; KWON; APOSTOLIDIS; SHETTY, 2008; KWON; VATTEN; SHETTY, 2006; MANCINI-FILHO, 2006; SHAHIDI; ALASALVAR; LIYANA-PATHIRANA, 2007). ... CHEN, D.; MILACIC, V.; CHEN, MS; WAN, S. B.; LAM, WH; HUO, C.; LANDIS-PIWOWAR, K. R.; CUI, QC ...

Grasas y Aceites, 2008

RESUMEN Fraccionamiento e interesterificacion del aceite de palma (Elaeis guineensis) cultivado e... more RESUMEN Fraccionamiento e interesterificacion del aceite de palma (Elaeis guineensis) cultivado en la amazonia peruana. En el presente trabajo se realizó el estudio de las características físicas y químicas del fruto de la palma aceitera procedente de la cuenca del Manití. (Región Loreto-Perú). Del mismo modo se realizó el fraccionamiento e interesterificación de las mezclas de aceite de palma y estearina en las proporciones. Sobre el aceite crudo y los productos se determinaron las propiedades físico-químicos y análisis de ácidos grasos mediante la cromatografía gaseosa. El aceite de palma presenta una concentración de ácido grasos saturados de 51,17% y cuando fraccionado a 25 °C, este se incrementa en la estearina a 54,31%. Los mejores productos para la industria de alimentos son las mezclas interesterificadas de estearina tanto sola como con sus mezclas con aceite de palma, dado que presentan puntos de fusión próximos a 37 °C.

Revista do Instituto de Medicina Tropical de São Paulo, 2012

In 1970, searching for the interspecies transmission of influenza viruses led to the first study ... more In 1970, searching for the interspecies transmission of influenza viruses led to the first study on influenza viruses in domestic animals. Birds and mammals, including human beings, are their natural hosts; however, other animals may also play a role in the virus epidemiology. The objective was to investigate the incidence of influenza viruses in adult dogs raised in rural (9, 19.56%) and urban (37, 80.43%) areas in the state of São Paulo, Brazil. Dog serum samples were examined for antibodies to influenza viruses by the hemagglutination inhibition (HI) test using the corresponding antigens from the circulating viruses in Brazil. Dogs from rural areas presented antibodies to influenza A H3N2, and influenza A H7N7 and H3N8. In rural areas, dog sera displayed mean titers as 94.37, 227.88, 168.14, 189.62 HIU/25 µL for subtypes H1N1, H3N2, H7N7, H3N8, respectively. About 84% and 92% of dogs from urban areas exhibited antibodies to human influenza A H1N1 and H3N2, respectively, with stat...

$Research paper thumbnail of Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux$

Brazilian Journal of Pharmaceutical Sciences, 2012

LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural ant... more LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural antioxidants have been studied as promising candidates. In the present study, the LDL oxidation inhibition activity of bioactive compounds from Halimeda incrassata seaweed. associated to antioxidant capacity, was evaluated in vitro. Experimental work was conducted with lyophilized aqueous extract and phenolic-rich fractions of the seaweed and their effect on LDL oxidation was evaluated using heparin-precipitated LDL (hep-LDL) with exposure to Cu2+ ions and AAPH as the free radical generator. H. incrassata had a protective effect for hep-LDL in both systems and the presence of phenolic compounds contributed to the activity where phenolic-rich fractions showed significant capacity for inhibition of oxidation mediated by Cu2+ ions. The observed effect could be related to the antioxidant potential of polar fractions evidenced by reducing activity and DPPH• radical scavenging. The results obtain...

Food Science and Technology

All solvents were analytical grade, used within their expiration dates and purchased from Merck (... more All solvents were analytical grade, used within their expiration dates and purchased from Merck (Darmstadt, Germany). The hexane and isopropanol used in this study were high performance liquid chromatography (HPLC) grade. Furthermore, the boron trifluoride-methanol solution 14% (BF 3 14%), fatty acid methyl ester (FAME) mix (C4:

Journal of Food and Nutrition Research

There is a dearth of efficient strategies to support postpartum among women in order to protect t... more There is a dearth of efficient strategies to support postpartum among women in order to protect them from chronic diseases in later life. Flaxseed oil (FO) is a food derived oil that has functional ingredients as alphalinolenic acid (ALA). The objective of this study was to investigate the influence of FO during postpartum period in body parameters and lipid profile in rats. After the delivery of their pups, rats were randomly divided into two groups: control-diet with soybean oil as fat source-or FO-diet with FO as a fat source. After 51 days offering experimental diets, each group was evaluated on body composition, intra-abdominal fat, serum lipid profile and polyunsaturated fatty acids. The diet based on FO recorded high serum levels of ALA (P<0.0001) and eicosapentaenoic fatty acids (P<0.05). The diet also recorded a decrease in gamma-linolenic (P<0.05), dihomo-gamma-linolenic (P<0.05) and arachidonic fatty acids (P<0.0001). These aforementioned results lead to the activation of metabolic and physiologic pathways that provided higher lean mass (P<0.05), lower results on total cholesterol (P<0.05) and low density cholesterol (P<0.05). Hence, consumption of FO during postpartum can promote lean mass and healthy body composition, better lipid profile and contribute to chronic disease prevention.

International Journal of Food Sciences and Nutrition

Nutrire

Background: Characterization of food lipids has triggered the development of applications for the... more Background: Characterization of food lipids has triggered the development of applications for the food and health industries. Thus, the lipid profiles of an increasing number of fruits and their seeds have been characterized and numerous bioactive components have been isolated. The bitter gourd seed oil has aroused great interest because it contains relevant amounts of conjugated fatty acid α-eleostearic acid (C18:3 9c11t13t), a positional and geometric isomer of α-linolenic acid. The aim of this paper was to evaluate the phytochemical composition and quality parameters of the seed oil of bitter gourd grown in Brazil. Methods: Bitter gourd was purchased from CEAGESP (Brazilian company of fresh food storages and warehouses). Seeds were lyophilized, and their oil was extracted using the Soxhlet and Folch extraction methods. The profiles of fatty acids and phytosterols were analyzed by gas chromatography, and the acidity and peroxide values were evaluated by methods of the American Oil Chemists' Society (AOCS). Results: The Soxhlet extraction (40 % w/w) resulted in a higher yield of bitter gourd seed oil than the Folch extraction (16 % w/w). For both methods, α-eleostearic acid (56 and 58 %) was the major lipid in bitter gourd seed oil, followed by stearic acid (C18:0; 32 and 27 %). The oil displayed high content of phytosterols (886 mg/100 g), mainly β-sitosterol, and low acidity and peroxide values. Conclusions: Bitter gourd seed oil from Brazil is an oil of good quality and its high contents of α-eleostearic acid and phytosterols with potential health-beneficial properties make it an attractive plant byproduct.

PloS one, 2017

Type 2 Diabetes mellitus (T2DM) is an evident growing disease that affects different cultures thr... more Type 2 Diabetes mellitus (T2DM) is an evident growing disease that affects different cultures throughout the world. T2DM occurs under the influence of three main factors: the genetic background, environmental and behavioral components. Obesity is strongly associated to the development of T2DM in the occident, while in the orient most of the diabetic patients are considered lean. Genetics may be a key factor in the development of T2DM in societies where obesity is not a recurrent public health problem. Herein, two different models of rats were used to understand their differences and reliability as experimental models to study the pathophysiology of T2DM, in two different approaches: the genetic (GK rats) and the environmental (HFD-induced obese rats) influences. GK rats were resistant to weight gain even though food/energy consumption (relative to body weight) was higher in this group. HFD, on the other hand, induced obesity in Wistar rats. White adipose tissue (WAT) expansion in th...

The Journal of nutritional biochemistry, Jan 11, 2017

Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to improve insulin sensitivity... more Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to improve insulin sensitivity and glucose homeostasis in animal models of insulin resistance, but the involved mechanisms still remain unresolved. In this study, we evaluated the effects of fish oil (FO), a source of n-3 PUFAs, on obesity, insulin resistance and muscle mitochondrial function in mice fed a high-fat diet (HFD). C57Bl/6 male mice, 8 weeks old, were divided into four groups: control diet (C), high-fat diet (H), C+FO (CFO) and H+FO (HFO). FO was administered by oral gavage (2 g/kg b.w.), three times a week, starting 4 weeks before diet administration until the end of the experimental protocol. HFD-induced obesity and insulin resistance associated with impaired skeletal muscle mitochondrial function, as indicated by decreased oxygen consumption, tricarboxylic acid cycle intermediate (TCAi) contents (citrate, α-ketoglutarate, malate and oxaloacetate), oxidative phosphorylation protein content and mitochond...

Scientific Reports

Obesogenic diets increase body weight and cause insulin resistance (IR), however, the association... more Obesogenic diets increase body weight and cause insulin resistance (IR), however, the association of these changes with the main macronutrient in the diet remains to be elucidated. Male C57BL/6 mice were fed with: control (CD), CD and sweetened condensed milk (HS), high-fat (HF), and HF and condensed milk (HSHF). After 2 months, increased body weight, glucose intolerance, adipocyte size and cholesterol levels were observed. As compared with CD, HS ingested the same amount of calories whereas HF and HSHF ingested less. HS had increased plasma AST activity and liver type I collagen. HF caused mild liver steatosis and hepatocellular damage. HF and HSHF increased LDL-cholesterol, hepatocyte and adipocyte hypertrophy, TNF-α by macrophages and decreased lipogenesis and adiponectin in adipose tissue (AT). HSHF exacerbated these effects, increasing IR, lipolysis, mRNA expression of F4/80 and leptin in AT, Tlr-4 in soleus muscle and IL-6, IL-1β, VCAM-1, and ICAM-1 protein in AT. The three obesogenic diets induced obesity and metabolic dysfunction. HS was more proinflammatory than the HF and induced hepatic fibrosis. The HF was more detrimental in terms of insulin sensitivity, and it caused liver steatosis. The combination HSHF exacerbated the effects of each separately on insulin resistance and AT inflammatory state. Obesity is an independent high risk factor for metabolic diseases such as type 2-diabetes and non-alcoholic fatty liver disease. A high intake of fat or sugar induces obesity and associated co-morbidities, such as insulin resistance, hyperglycaemia and dyslipidaemia in humans and experimental animals 1, 2. Mice fed high-energy food are used as an experimental model to investigate the mechanisms associated with dysfunction in metabolism 3-5. Several investigators have combined macronutrients fat and sugar (fructose) to induce the main features of metabolic disorders observed in humans 6, 7. The different compositions of energy-dense foods can increase weight and lead to insulin resistance with varying intensities. Maioli et al. 6 reported that in C57BL/6 mice, compared with other obesogenic diets that have been reported to induce obesity or metabolic disorders, a diet rich in sucrose and lipids induces a more prominent body weight gain and increase in fasting blood glucose levels. The authors reported a reduced frequency of regulatory T cells as well as decreased levels of anti-inflammatory cytokines

$Research paper thumbnail of Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers$

Brazilian Journal of Pharmaceutical Sciences

Peptic ulcers are an important pathology, and the search for safer and more effective treatment m... more Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE 50 =41.91 µg/mL) by DPPH in comparison with Trolox ® and 11.33 mmol Trolox ® equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids. Uniterms: Gastric ulcer/study/rats. Gastric ulcer/treatment. Kalanchoe pinnata/chromatographic profile. Kalanchoe pinnata/gastroprotective activity. Quercetrin. Quercetin 3-O-α-L-arabinopyranosyl-(1→2)α-L-rhamnopyranoside.

Journal of Food Science and Technology

The conditions for the solid-liquid extraction of the antioxidant polyphenol compounds from yello... more The conditions for the solid-liquid extraction of the antioxidant polyphenol compounds from yellow passion fruit seeds were optimized by response surface methodology with the following variables as the extraction parameters: extraction time (12.8-147.2 min), ethanol concentration (13-97%), and temperature (16.4-83.6 °C). The polyphenol content and antioxidant capacity, which were assessed by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, oxygen radical absorbance capacity, β-carotene bleaching assay, and ferric reducing antioxidant power assay, were considered dependent variables. The association of the dependent variables was effective for explaining the effect of the independent variables within a determination coefficient (R2) range of 0.88-0.96. A moderate-to-strong correlation for the polyphenol content and antioxidant capacity by the investigated methods was established, and optimized conditions were employed to maximize this response. Extraction was carried out at 80 °C using 70% ethanol concentration for 30 min, which was the most efficient condition to obtain an extract with high concentrations of polyphenolic compounds (3.12 g gallic acid equivalent/100 g seed dry basis) and a strong antioxidant capacity. The stilbene piceatannol was the major compound identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (3.68 g/100 g seed dry basis). These results reinforce that agro-industrial waste demonstrates potential as a source of bioactive compounds, with implications in human health as well as in food and chemical industries.

Lipids, Apr 21, 2017

In women, bone mass undergoes changes during pregnancy and the postpartum period, which has a ris... more In women, bone mass undergoes changes during pregnancy and the postpartum period, which has a risk for subsequent development of osteoporosis. Thus, the present study aims to evaluate the effects of flaxseed flour in femur quality during post-weaning of dam rats. After weaning, the rats were divided into control (C, n = 7) and experimental (F, n = 7) groups treated with a diet containing 25 g of flaxseed flour in the lactating period and 15 g in the maintenance period. After 51 days post-partum, serum hormone, fatty acids composition, bone compartments, computed tomography, and biomechanical analyses were determined. Food intake, length, body mass, hormone analysis, and total bone compartments showed similar results. For biomechanical and computed tomography analysis and fatty acids composition, the F group showed higher maximum force (+12%, p < 0.05), breaking strength (+25%, p < 0.05), rigidity (+17%,…

Experimental Physiology, 2016

Oleic and linoleic acids control fibroblast proliferation and myogenic differentiation in vitro, ... more Oleic and linoleic acids control fibroblast proliferation and myogenic differentiation in vitro, however, there was no study in skeletal muscle in vivo. The aim of this study was then to evaluate the effects of either oleic or linoleic acid on muscle content of fibrous tissue (collagen deposition) and contractile function recovery in rat gastrocnemius muscle after being severely injured by laceration. Rats were supplemented with either oleic or linoleic acid during four weeks after laceration (0.44 g per kg body weight per day). Muscle injury led to an increase in oleic/stearic acid and palmitoleic/palmitic acid ratios, suggesting an increase in Δ(9) desaturase activity. Increased fibrous tissue deposition and reduced isotonic and tetanic specific forces and resistance to fatigue were observed in the injured muscle. Supplementation with linoleic acid raised the content of eicosadienoic (20:2, n-6) and arachidonic (20:4, n-6) acids, reduced muscle mass and fiber cross-sectional areas (CSA), increased fibrous tissue deposition and further reduced isotonic and tetanic specific forces and resistance to fatigue induced by laceration. Supplementation with oleic acid raised the content of docosahexaenoic acid (DHA, 22:6, n-3), abolished the increase in fibrous tissue area and the decrease in isotonic and tetanic specific forces and resistance to fatigue induced by muscle injury. We concluded that supplementation with linoleic acid impairs muscle regeneration and increases fibrous tissue deposition, resulting in impaired contractile function recovery. Oleic acid supplementation reduced fibrous tissue deposition and improved contractile function recovery, attenuating the tissue damage caused by muscle injury. This article is protected by copyright. All rights reserved.

Hig Aliment, Aug 1, 2006

... para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 446600. Autor: Araújo,Fa... more ... para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 446600. Autor: Araújo,Fabiana Amaral; Mancini Filho, Jorge. Título: Compostos bioativos do café e seus benefícios à saúde / Bioactive compounds of the coffee and its benefits to the health. Fonte: Hig. ...

Revista Do Instituto Adolfo Lutz, 2005

CEP